Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,085,600 G→T 12.0% Q43K (CAA→AAA)  frvB ← putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,085,6000GT12.0% 1402.7 / 115.2 409Q43K (CAA→AAA) frvBputative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (179/180);  new base T (18/32);  total (197/212)
Fisher's exact test for biased strand distribution p-value = 7.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

CGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGG  >  CP009273/4085451‑4085749
                                                                                                                                                     |                                                                                                                                                     
 gCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGa                                                                                                                                                      >  2:3720501/1‑150 (MQ=255)
   cTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGt                                                                                                                                                    >  1:1740795/1‑150 (MQ=255)
     gggAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGttt                                                                                                                                                  <  2:1333597/150‑1 (MQ=255)
     gggAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGttt                                                                                                                                                  <  2:2218319/150‑1 (MQ=255)
      ggAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTc                                                                                                                                                 <  2:2673095/150‑1 (MQ=255)
       gagaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTcc                                                                                                                                                <  1:1805584/150‑1 (MQ=255)
       gagaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTcc                                                                                                                                                >  2:4491118/1‑150 (MQ=255)
         gaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCAc                                                                                                                                              <  2:3589909/150‑1 (MQ=255)
         gaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCAc                                                                                                                                              <  1:3261749/150‑1 (MQ=255)
          aTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACt                                                                                                                                             <  2:1825011/150‑1 (MQ=255)
           tGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACtt                                                                                                                                            <  1:4514019/150‑1 (MQ=255)
           tGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACtt                                                                                                                                            >  2:4726032/1‑150 (MQ=255)
             gCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACtttt                                                                                                                                          <  1:4202003/150‑1 (MQ=255)
             gCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACtttt                                                                                                                                          <  1:659850/150‑1 (MQ=255)
              cAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTa                                                                                                                                         <  1:38017/150‑1 (MQ=255)
              cAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTa                                                                                                                                         >  2:435049/1‑150 (MQ=255)
               aaTCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTAt                                                                                                                                        <  2:3455309/150‑1 (MQ=255)
               aaTCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTAt                                                                                                                                        >  1:1424504/1‑150 (MQ=255)
               aaTCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTAt                                                                                                                                        <  2:2996425/150‑1 (MQ=255)
                 tctcATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATgg                                                                                                                                      >  2:512298/1‑150 (MQ=255)
                     aTAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATg                                                                                                                                  >  1:2298275/1‑150 (MQ=255)
                      tAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGa                                                                                                                                 <  1:3579233/150‑1 (MQ=255)
                       aaaCTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGAc                                                                                                                                >  2:2804352/1‑150 (MQ=255)
                       aaaCTTTCTTCCCGGCAAACCGCGCGCGATCATCACAGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTTGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGAc                                                                                                                                <  1:3113805/150‑1 (MQ=255)
                          cTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCga                                                                                                                             >  1:4319517/1‑150 (MQ=255)
                           tttCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCgag                                                                                                                            >  2:4604145/1‑150 (MQ=255)
                             tcttcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCgagag                                                                                                                          >  2:3641140/1‑150 (MQ=255)
                                tcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAAc                                                                                                                       <  2:2024295/150‑1 (MQ=255)
                                  ccGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACgg                                                                                                                     >  2:1086671/1‑150 (MQ=255)
                                  ccGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACgg                                                                                                                     >  1:870290/1‑150 (MQ=255)
                                   cGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACggg                                                                                                                    >  1:1953210/1‑150 (MQ=255)
                                        aaCCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTc                                                                                                               <  1:2544477/150‑1 (MQ=255)
                                          ccGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTg                                                                                                             <  2:544346/150‑1 (MQ=255)
                                              gcgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTcc                                                                                                         <  1:391075/150‑1 (MQ=255)
                                               cgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCa                                                                                                        >  2:1506055/1‑150 (MQ=255)
                                                gcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAg                                                                                                       <  2:3976859/150‑1 (MQ=255)
                                                gcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAg                                                                                                       >  2:687517/1‑150 (MQ=255)
                                                gcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAg                                                                                                       <  2:1590244/150‑1 (MQ=255)
                                                 cgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAgg                                                                                                      <  1:1016805/150‑1 (MQ=255)
                                                 cgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAgg                                                                                                      <  1:3401809/150‑1 (MQ=255)
                                                   atcatcACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGc                                                                                                    >  2:1798519/1‑150 (MQ=255)
                                                    tcatcaCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCt                                                                                                   >  1:2033920/1‑150 (MQ=255)
                                                     catcaCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCtt                                                                                                  <  2:2627514/150‑1 (MQ=255)
                                                     catcaCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCtt                                                                                                  <  2:1960136/150‑1 (MQ=255)
                                                         aCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGc                                                                                              >  2:352099/1‑150 (MQ=255)
                                                           cGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGcca                                                                                            >  1:477792/1‑150 (MQ=255)
                                                           cGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGcca                                                                                            >  1:983806/1‑150 (MQ=255)
                                                              tCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGccacca                                                                                         >  2:2839680/1‑150 (MQ=255)
                                                                aGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATg                                                                                       <  2:2157864/150‑1 (MQ=255)
                                                                  gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTa                                                                                     <  1:3735703/150‑1 (MQ=255)
                                                                   ccACGCCCGGTAGCGAGACTGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAg                                                                                    >  2:3377833/1‑150 (MQ=255)
                                                                   ccACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAg                                                                                    >  1:2568251/1‑150 (MQ=255)
                                                                    cACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAgg                                                                                   >  2:3911866/1‑150 (MQ=255)
                                                                      cGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGgtg                                                                                 <  2:4198610/150‑1 (MQ=255)
                                                                          cGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAg                                                                             <  2:3195336/150‑1 (MQ=255)
                                                                           ggTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGc                                                                            >  2:3300380/1‑150 (MQ=255)
                                                                            gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCg                                                                           >  1:3205291/1‑150 (MQ=255)
                                                                                 gagaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATccc                                                                      <  2:2660089/150‑1 (MQ=255)
                                                                                        aCGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                               <  1:3072982/150‑1 (MQ=255)
                                                                                            aaTCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGt                                                           <  2:3877213/150‑1 (MQ=255)
                                                                                              tCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCGGTTg                                                         <  1:118081/150‑1 (MQ=255)
                                                                                               cGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTgg                                                        <  1:3149768/150‑1 (MQ=255)
                                                                                                ggCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                       >  1:1116986/1‑150 (MQ=255)
                                                                                                ggCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                       <  1:72110/150‑1 (MQ=255)
                                                                                                    gCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATc                                                   >  1:275243/1‑150 (MQ=255)
                                                                                                     cGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATcg                                                  <  1:4145581/150‑1 (MQ=255)
                                                                                                        cAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGa                                               >  1:1617959/1‑150 (MQ=255)
                                                                                                         aaTCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGAc                                              >  2:2365754/1‑150 (MQ=255)
                                                                                                          aTCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                             >  1:1234253/1‑150 (MQ=255)
                                                                                                          aTCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                             <  1:3657801/150‑1 (MQ=255)
                                                                                                            ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAAt                                           >  2:1908639/1‑150 (MQ=255)
                                                                                                             tctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATa                                          <  2:774773/150‑1 (MQ=255)
                                                                                                             tctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATa                                          <  2:3141693/150‑1 (MQ=255)
                                                                                                             tctTCGCTGGATAAGCGGTTTTCAAAGCCACTGGACCCTTGAGTTTCCACTTTTATTGTATGACCGAGAGTACGGGCTTTCTGTTACAGGGCTTCCGCCACCATGTAGGTGTGAGGGATCCAGGCGGGGCGGTTGGTGATCGCGACAATa                                          >  1:1705365/1‑150 (MQ=255)
                                                                                                              ctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATAc                                         <  1:2958697/150‑1 (MQ=255)
                                                                                                              ctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATAc                                         <  1:4050627/150‑1 (MQ=255)
                                                                                                                tCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGt                                       >  2:4123299/1‑150 (MQ=255)
                                                                                                                  gCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTaa                                     >  2:2697211/1‑150 (MQ=255)
                                                                                                                    tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAgg                                   >  1:1346877/1‑150 (MQ=255)
                                                                                                                    tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAgg                                   <  2:1450808/150‑1 (MQ=255)
                                                                                                                     ggATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGa                                  >  2:1246975/1‑150 (MQ=255)
                                                                                                                         aaGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGAc                              <  2:4673946/150‑1 (MQ=255)
                                                                                                                         aaGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGAc                              <  2:171258/150‑1 (MQ=255)
                                                                                                                          aGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACt                             >  1:3866351/1‑150 (MQ=255)
                                                                                                                          aGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACt                             <  1:1263724/150‑1 (MQ=255)
                                                                                                                                  tCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGcc                     <  1:550882/150‑1 (MQ=255)
                                                                                                                                   ccaCGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCAGGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCa                    <  1:3429725/148‑1 (MQ=255)
                                                                                                                                    aaCGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCAc                   >  1:1600145/1‑150 (MQ=255)
                                                                                                                                     aCGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACt                  <  2:2501580/150‑1 (MQ=255)
                                                                                                                                        ccACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTcc               <  1:1464845/150‑1 (MQ=255)
                                                                                                                                         cACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCt              <  2:1236409/150‑1 (MQ=255)
                                                                                                                                            tGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTAt           <  1:2127444/150‑1 (MQ=255)
                                                                                                                                              gACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGa         >  2:1429368/1‑150 (MQ=255)
                                                                                                                                                 ccTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt      <  1:1530128/150‑1 (MQ=255)
                                                                                                                                                 ccTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt      <  1:4646920/150‑1 (MQ=255)
                                                                                                                                                 acTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGTTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt      <  1:1297765/149‑1 (MQ=255)
                                                                                                                                                  cTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTg     <  2:3761536/150‑1 (MQ=255)
                                                                                                                                                  cTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTg     >  1:1124224/1‑150 (MQ=255)
                                                                                                                                                   ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGa    <  1:4084420/150‑1 (MQ=255)
                                                                                                                                                    tGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAg   <  1:2812904/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGG  >  CP009273/4085451‑4085749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A