Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,085,600 G→T 26.9% Q43K (CAA→AAA)  frvB ← putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,085,6000GT26.9% 561.6 / 202.3 266Q43K (CAA→AAA) frvBputative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (91/103);  new base T (36/36);  total (127/139)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGG  >  CP009273/4085451‑4085749
                                                                                                                                                     |                                                                                                                                                     
cGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTACTCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTg                                                                                                                                                       >  1:801767/1‑150 (MQ=255)
cGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTg                                                                                                                                                       >  2:684897/1‑150 (MQ=255)
    tGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGtt                                                                                                                                                   <  1:2432631/150‑1 (MQ=255)
        agaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCa                                                                                                                                               >  1:3821218/1‑150 (MQ=255)
        agaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCa                                                                                                                                               >  1:4247400/1‑150 (MQ=255)
         gaTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCAc                                                                                                                                              <  2:1968068/150‑1 (MQ=255)
          aTGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACt                                                                                                                                             <  1:3449522/150‑1 (MQ=255)
              cAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTa                                                                                                                                         <  1:1600323/150‑1 (MQ=255)
               aaTCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTAt                                                                                                                                        >  1:131110/1‑150 (MQ=255)
                    cATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTAt                                                                                                                                   >  2:3975236/1‑150 (MQ=255)
                    cATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTAAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTAt                                                                                                                                   >  2:154015/1‑150 (MQ=255)
                     aTAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATg                                                                                                                                  >  1:3637026/1‑150 (MQ=255)
                     aTAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATg                                                                                                                                  <  2:1507514/150‑1 (MQ=255)
                     aTAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATg                                                                                                                                  <  2:1514905/150‑1 (MQ=255)
                     aTAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATg                                                                                                                                  <  2:3803750/150‑1 (MQ=255)
                      tAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGa                                                                                                                                 <  1:815608/150‑1 (MQ=255)
                        aaCTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGAcc                                                                                                                               <  2:3927770/150‑1 (MQ=255)
                        aaCTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGAcc                                                                                                                               >  2:3127651/1‑150 (MQ=255)
                         aCTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCg                                                                                                                              <  2:26268/150‑1 (MQ=255)
                          cTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCga                                                                                                                             >  1:907989/1‑150 (MQ=255)
                             tcttcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCgagag                                                                                                                          >  2:3828336/1‑150 (MQ=255)
                              cttcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCgagaga                                                                                                                         >  2:4953227/1‑150 (MQ=255)
                              cttcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCgagaga                                                                                                                         >  1:327382/1‑150 (MQ=255)
                              aatcCCGGCAAACCGCGCGCGATCATCACCGCTCAGTCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCCACGCCACTGGACCCTAGAGTTACCACTTTTATGGTATGACCgagaga                                                                                                                         >  2:4860355/3‑150 (MQ=255)
                                tcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAAc                                                                                                                       <  1:988062/150‑1 (MQ=255)
                                tcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAAc                                                                                                                       <  1:4908050/150‑1 (MQ=255)
                                tcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAAc                                                                                                                       <  2:289816/150‑1 (MQ=255)
                                tcCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAAc                                                                                                                       >  2:4002783/1‑150 (MQ=255)
                                 cccGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACg                                                                                                                      >  1:3119969/1‑150 (MQ=255)
                                 cccGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACg                                                                                                                      <  1:3322500/150‑1 (MQ=255)
                                  ccGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACgg                                                                                                                     <  1:2267656/150‑1 (MQ=255)
                                       aaaCCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCttt                                                                                                                >  1:2549116/1‑150 (MQ=255)
                                         aCCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCt                                                                                                              <  2:4643298/150‑1 (MQ=255)
                                           cgcgcgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGt                                                                                                            <  1:1477543/150‑1 (MQ=255)
                                             cgcgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTc                                                                                                          <  1:2856138/150‑1 (MQ=255)
                                              gcgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTcc                                                                                                         <  2:1337931/150‑1 (MQ=255)
                                               cgcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCa                                                                                                        <  1:124481/150‑1 (MQ=255)
                                                gcgATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAg                                                                                                       <  2:2200181/150‑1 (MQ=255)
                                                  gATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAggg                                                                                                     >  1:4737770/1‑150 (MQ=255)
                                                     catcaCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCtt                                                                                                  <  1:4489631/150‑1 (MQ=255)
                                                       tcaCCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTcc                                                                                                <  1:2541012/150‑1 (MQ=255)
                                                            gCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGccac                                                                                           <  1:1030339/150‑1 (MQ=255)
                                                             cTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGccacc                                                                                          <  1:4845753/150‑1 (MQ=255)
                                                                    cACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAgg                                                                                   >  1:526153/1‑150 (MQ=255)
                                                                     aCGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGgt                                                                                  >  2:3005779/1‑150 (MQ=255)
                                                                     aCGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGgt                                                                                  >  2:2449098/1‑150 (MQ=255)
                                                                        cccGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGtgtg                                                                               <  2:2922702/150‑1 (MQ=255)
                                                                        cccGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGtgtg                                                                               <  1:2276119/150‑1 (MQ=255)
                                                                            gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCg                                                                           <  1:2706922/150‑1 (MQ=255)
                                                                              aGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGAt                                                                         >  2:1127226/1‑150 (MQ=255)
                                                                                 gagaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATccc                                                                      >  2:4814862/1‑150 (MQ=255)
                                                                                 gagaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATccc                                                                      >  2:4815189/1‑150 (MQ=255)
                                                                                  agaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCcg                                                                     <  2:2701095/150‑1 (MQ=255)
                                                                                  agaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCcg                                                                     >  2:750495/1‑150 (MQ=255)
                                                                                  agaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCcg                                                                     >  1:2165624/1‑150 (MQ=255)
                                                                                  agaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTATTATGGTATGACCGAGAGAACGGGCTTTCTGTTCAAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCcg                                                                     >  2:2058809/1‑150 (MQ=255)
                                                                                   gaATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCcgg                                                                    >  1:4619731/1‑150 (MQ=255)
                                                                                       gACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCggg                                                                >  1:2017269/1‑150 (MQ=255)
                                                                                         cGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGc                                                              <  1:4397030/150‑1 (MQ=255)
                                                                                           tAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTATGTGTGAGCGATCCCGGCGGGGCAg                                                            >  1:4010709/1‑150 (MQ=255)
                                                                                            aaTCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGt                                                           <  2:502798/150‑1 (MQ=255)
                                                                                             aTCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCCGtt                                                          >  2:1399482/1‑150 (MQ=255)
                                                                                             aTCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGtt                                                          >  2:3514128/1‑150 (MQ=255)
                                                                                                   aGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGAt                                                    >  2:2040233/1‑150 (MQ=255)
                                                                                                   aGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGAt                                                    >  2:3200460/1‑150 (MQ=255)
                                                                                                         aaTCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCCGTTGGTGATCGCGAc                                              >  2:4390460/1‑150 (MQ=255)
                                                                                                            ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAAt                                           >  1:3839603/1‑150 (MQ=255)
                                                                                                              ctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATAc                                         <  2:224950/150‑1 (MQ=255)
                                                                                                                tCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGt                                       <  2:103140/150‑1 (MQ=255)
                                                                                                                   cTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAg                                    <  1:2708222/150‑1 (MQ=255)
                                                                                                                   cTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAg                                    <  1:1862196/150‑1 (MQ=255)
                                                                                                                    tGGATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAgg                                   <  1:2956635/150‑1 (MQ=255)
                                                                                                                     ggATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCTCTTTTATGGTATGACCGAGAGAACGGGCGTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGTTCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGa                                  <  2:4413751/150‑1 (MQ=255)
                                                                                                                      gATAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGAt                                 <  1:4998202/150‑1 (MQ=255)
                                                                                                                       aTAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATg                                <  2:4238287/150‑1 (MQ=255)
                                                                                                                        tAAGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGa                               >  2:1347824/1‑150 (MQ=255)
                                                                                                                        tAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGa                               <  2:2949350/150‑1 (MQ=255)
                                                                                                                        tAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGa                               >  1:1086596/1‑150 (MQ=255)
                                                                                                                         aaGCGGTTTTCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGAc                              <  2:3583007/150‑1 (MQ=255)
                                                                                                                         aaGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGAc                              <  1:2196754/150‑1 (MQ=255)
                                                                                                                            cGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTcc                           <  1:3530597/150‑1 (MQ=255)
                                                                                                                             ggTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCa                          >  2:1716583/1‑150 (MQ=255)
                                                                                                                               ttttCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATc                        >  2:2679412/1‑150 (MQ=255)
                                                                                                                                tttCAACGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTACAGGGCTTCCGCCACCAAGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCg                       >  1:938155/1‑150 (MQ=255)
                                                                                                                                  tCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGcc                     <  2:802453/150‑1 (MQ=255)
                                                                                                                                   cAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCa                    >  1:591212/1‑150 (MQ=255)
                                                                                                                                     aCGCCACTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACt                  >  2:2329021/1‑150 (MQ=255)
                                                                                                                                     aCGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACt                  <  2:922138/150‑1 (MQ=255)
                                                                                                                                      cGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACtt                 <  2:1621234/150‑1 (MQ=255)
                                                                                                                                      cGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACtt                 >  1:374520/1‑150 (MQ=255)
                                                                                                                                       gCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTc                <  1:408832/150‑1 (MQ=255)
                                                                                                                                       gCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGGTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTc                <  2:1162176/150‑1 (MQ=255)
                                                                                                                                        ccACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTcc               >  2:1193900/1‑150 (MQ=255)
                                                                                                                                          aCTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCtt             >  2:255862/1‑150 (MQ=255)
                                                                                                                                          aCTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCtt             <  2:5172657/150‑1 (MQ=255)
                                                                                                                                           cTGGACCCTTTAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTa            <  2:1189543/150‑1 (MQ=255)
                                                                                                                                           cTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTa            >  1:3174064/1‑150 (MQ=255)
                                                                                                                                             ggACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATg          >  1:753424/1‑150 (MQ=255)
                                                                                                                                             ggACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATg          >  1:2334023/1‑150 (MQ=255)
                                                                                                                                                     gAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAgg  <  2:4704347/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCTGGGAGATGGCAATCTCATAAACTTTCTTCCCGGCAAACCGCGCGCGATCATCACCGCTCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGG  >  CP009273/4085451‑4085749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A