Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,767,963 C→A 21.5% P399T (CCT→ACT)  ydiN → MFS transporter superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,767,9630CA21.5% 682.7 / 141.1 260P399T (CCT→ACT) ydiNMFS transporter superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (108/96);  new base A (21/35);  total (129/131)
Fisher's exact test for biased strand distribution p-value = 4.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATCGC  >  CP009273/1767814‑1768112
                                                                                                                                                     |                                                                                                                                                     
gACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTc                                                                                                                                                       <  2:5017114/150‑1 (MQ=255)
gACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTc                                                                                                                                                       >  1:4159883/1‑150 (MQ=255)
 aCTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTcc                                                                                                                                                      >  2:2437377/1‑150 (MQ=255)
     gCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGaa                                                                                                                                                  >  2:423965/1‑150 (MQ=255)
       tAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGaaaa                                                                                                                                                <  1:4830161/150‑1 (MQ=255)
        aaCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAat                                                                                                                                               <  1:5031339/150‑1 (MQ=255)
         aCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAatg                                                                                                                                              <  2:4355888/150‑1 (MQ=255)
            ttGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAatgatg                                                                                                                                           <  2:2342381/150‑1 (MQ=255)
            ttGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAatgatg                                                                                                                                           <  2:2342513/150‑1 (MQ=255)
             tGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGAtgt                                                                                                                                          >  2:4863854/1‑150 (MQ=255)
                 aTTCCACTGATTACCGGTTATCTGTCGAACATCGTCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAAGTATTGTTTTTATCCGCTATTACCGCGTTTTAATTATTCCTGAAAATGATGAGCgg                                                                                                                                      >  2:3109163/1‑150 (MQ=255)
                 aTTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCgg                                                                                                                                      >  2:3110205/1‑150 (MQ=255)
                    ccACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGttt                                                                                                                                   <  2:2526934/150‑1 (MQ=255)
                    ccACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGttt                                                                                                                                   >  1:3325640/1‑150 (MQ=255)
                    ccACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGttt                                                                                                                                   <  1:4204813/150‑1 (MQ=255)
                      aCTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTgg                                                                                                                                 >  2:3176464/1‑150 (MQ=255)
                      aCTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTgg                                                                                                                                 <  1:358212/150‑1 (MQ=255)
                       cTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGc                                                                                                                                >  1:906121/1‑150 (MQ=255)
                         gATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGa                                                                                                                              <  1:10980/150‑1 (MQ=255)
                         gATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATGGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGa                                                                                                                              <  1:710722/150‑1 (MQ=255)
                          aTTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAg                                                                                                                             >  2:4433002/1‑150 (MQ=255)
                           ttACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGc                                                                                                                            >  2:1951925/1‑150 (MQ=255)
                                ggTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTaaa                                                                                                                       >  2:4754089/1‑150 (MQ=255)
                                ggTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTaaa                                                                                                                       <  1:4817268/150‑1 (MQ=255)
                                 gTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAAt                                                                                                                      <  1:367009/150‑1 (MQ=255)
                                  ttATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTACTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtt                                                                                                                     <  2:1233792/150‑1 (MQ=255)
                                   tATCGGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAAttt                                                                                                                    <  2:4631880/150‑1 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtttt                                                                                                                   >  1:2709797/1‑150 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtttt                                                                                                                   <  2:3902953/150‑1 (MQ=255)
                                        gTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAc                                                                                                               >  1:4777625/1‑150 (MQ=255)
                                          cGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAccc                                                                                                             <  1:565599/150‑1 (MQ=255)
                                          cGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAccc                                                                                                             <  1:565258/150‑1 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                            >  2:4820237/1‑150 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                            >  2:2053541/1‑150 (MQ=255)
                                            aaCATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCgg                                                                                                           <  2:1391362/150‑1 (MQ=255)
                                                 cGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaaa                                                                                                      >  2:4133684/1‑150 (MQ=255)
                                                     cTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAAcaca                                                                                                  >  2:3201755/1‑150 (MQ=255)
                                                     cTGAAATATATAATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTAACGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGAGAGCGTAAATTTATCACCCGGTTAAAcaca                                                                                                  >  2:3611463/1‑150 (MQ=255)
                                                        cAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAtt                                                                                               <  2:4398454/150‑1 (MQ=255)
                                                        cAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAtt                                                                                               >  1:4881261/1‑150 (MQ=255)
                                                          atatatCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTaa                                                                                             <  1:3962707/150‑1 (MQ=255)
                                                            atatCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGc                                                                                           <  1:4989262/150‑1 (MQ=255)
                                                             tatCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCa                                                                                          <  2:3272677/150‑1 (MQ=255)
                                                               tCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATa                                                                                        <  2:2874215/150‑1 (MQ=255)
                                                               tCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATa                                                                                        >  1:2018020/1‑150 (MQ=255)
                                                                cATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATag                                                                                       <  2:1546535/150‑1 (MQ=255)
                                                                 aTTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGAGGTTTGGCGAGCGTAAATTATGCACCAGGTTAAACACAATTAAGCATaga                                                                                      >  1:2330611/1‑150 (MQ=255)
                                                                       ctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTCAGCATAGAGGTTaa                                                                                >  2:3986505/1‑150 (MQ=255)
                                                                       ctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTaa                                                                                <  1:5119306/150‑1 (MQ=255)
                                                                       ctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTaa                                                                                >  1:1913071/1‑150 (MQ=255)
                                                                         cGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCCATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAATAAGCATCGAGGTTACAg                                                                              >  1:3205634/1‑150 (MQ=255)
                                                                         cGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAg                                                                              <  1:3171444/150‑1 (MQ=255)
                                                                          gATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGATTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAgg                                                                             <  2:1286224/150‑1 (MQ=255)
                                                                            ttttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAg                                                                           <  1:2928872/150‑1 (MQ=255)
                                                                             tttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGt                                                                          <  1:769633/150‑1 (MQ=255)
                                                                             tttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGt                                                                          >  2:333987/1‑150 (MQ=255)
                                                                              ttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGtt                                                                         <  2:1990487/150‑1 (MQ=255)
                                                                                aCTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATGACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTaa                                                                       <  2:1940878/150‑1 (MQ=255)
                                                                                 cTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAAt                                                                      >  1:1419267/1‑150 (MQ=255)
                                                                                   ttCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTa                                                                    >  2:4391625/1‑150 (MQ=255)
                                                                                      gcgcTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATgg                                                                 <  1:1780360/150‑1 (MQ=255)
                                                                                         ctgctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATg                                                              <  1:2215980/150‑1 (MQ=255)
                                                                                           gctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGtt                                                            <  2:2186813/150‑1 (MQ=255)
                                                                                            ctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTa                                                           >  2:2166038/1‑150 (MQ=255)
                                                                                            ctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTa                                                           >  1:528792/1‑150 (MQ=255)
                                                                                             tgGCCCTGAGTACCGCAATTATTGTTTTGATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAc                                                          <  1:3447751/150‑1 (MQ=255)
                                                                                              ggCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAcc                                                         >  1:3187090/1‑150 (MQ=255)
                                                                                                cccTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGc                                                       >  2:5128615/1‑150 (MQ=255)
                                                                                                 ccTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCa                                                      <  2:5094463/150‑1 (MQ=255)
                                                                                                 ccTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCa                                                      <  1:4058022/150‑1 (MQ=255)
                                                                                                 ccTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCa                                                      >  1:1843141/1‑150 (MQ=255)
                                                                                                  cTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCaa                                                     <  2:730457/150‑1 (MQ=255)
                                                                                                     aTTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAAt                                                  >  2:2282416/1‑150 (MQ=255)
                                                                                                     aTTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAAt                                                  <  2:1461815/150‑1 (MQ=255)
                                                                                                         ccGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGa                                              <  2:3710418/150‑1 (MQ=255)
                                                                                                         ccGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGa                                              <  2:3796403/150‑1 (MQ=255)
                                                                                                           gCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAat                                            <  1:1013670/150‑1 (MQ=255)
                                                                                                            cAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAatt                                           >  2:2688342/1‑150 (MQ=255)
                                                                                                            cAATTATTGTTTTTATCCGCTAGTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAatt                                           <  2:4516581/150‑1 (MQ=255)
                                                                                                             aaTTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattg                                          <  1:4464596/150‑1 (MQ=255)
                                                                                                                tattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgatt                                       <  1:4323835/150‑1 (MQ=255)
                                                                                                                 attGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgattg                                      >  2:2490955/1‑150 (MQ=255)
                                                                                                                    gTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGt                                   <  1:1623141/150‑1 (MQ=255)
                                                                                                                     tttttATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGtt                                  >  2:4290017/1‑150 (MQ=255)
                                                                                                                      ttttATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTg                                 >  2:3016345/1‑150 (MQ=255)
                                                                                                                      ttttATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTg                                 <  1:2864191/150‑1 (MQ=255)
                                                                                                                        ttATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGAt                               >  2:1786065/1‑150 (MQ=255)
                                                                                                                          aTCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATgg                             >  2:4840029/1‑150 (MQ=255)
                                                                                                                           tCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGc                            >  2:2807610/1‑150 (MQ=255)
                                                                                                                           tCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGc                            >  1:4487256/1‑150 (MQ=255)
                                                                                                                            ccGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcc                           >  2:2519387/1‑150 (MQ=255)
                                                                                                                              gCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGccta                         >  2:855149/1‑150 (MQ=255)
                                                                                                                                   tACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatccta                    <  1:1572630/150‑1 (MQ=255)
                                                                                                                                    aCCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcctat                   <  2:2077180/150‑1 (MQ=255)
                                                                                                                                      cgcgTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCtatcctatcc                 <  2:3743343/150‑1 (MQ=255)
                                                                                                                                      cgcgTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCtatcctatcc                 <  1:423965/150‑1 (MQ=255)
                                                                                                                                       gcgTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCg                <  2:3495348/150‑1 (MQ=255)
                                                                                                                                       gcgTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCg                <  1:3811898/150‑1 (MQ=255)
                                                                                                                                        cgTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGc               >  1:2567402/1‑150 (MQ=255)
                                                                                                                                         gTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGcc              >  1:154694/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATCGC  >  CP009273/1767814‑1768112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A