Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,767,963 C→A 15.4% P399T (CCT→ACT)  ydiN → MFS transporter superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,767,9630CA15.4% 771.5 / 83.0 238P399T (CCT→ACT) ydiNMFS transporter superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (96/105);  new base A (12/25);  total (108/130)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATC  >  CP009273/1767814‑1768110
                                                                                                                                                     |                                                                                                                                                   
   tGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTg                                                                                                                                                  <  2:707809/150‑1 (MQ=255)
   tGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTg                                                                                                                                                  >  1:2322975/1‑150 (MQ=255)
   tGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTg                                                                                                                                                  <  1:1591749/150‑1 (MQ=255)
    ggCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGa                                                                                                                                                 >  1:275836/1‑150 (MQ=255)
     gCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGaa                                                                                                                                                <  2:622372/150‑1 (MQ=255)
        aaCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAat                                                                                                                                             <  2:2213348/150‑1 (MQ=255)
          cTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAatga                                                                                                                                           <  1:4493441/150‑1 (MQ=255)
                   tCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGtt                                                                                                                                  <  1:3500750/150‑1 (MQ=255)
                    ccACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGttt                                                                                                                                 <  1:2939521/150‑1 (MQ=255)
                       cTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGc                                                                                                                              <  2:2828761/150‑1 (MQ=255)
                       cTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGc                                                                                                                              <  2:2182625/150‑1 (MQ=255)
                        tGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCg                                                                                                                             <  2:1813859/150‑1 (MQ=255)
                         gATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGa                                                                                                                            >  1:1271512/1‑150 (MQ=255)
                           ttACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGc                                                                                                                          <  1:1382963/150‑1 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAAATATTGTTTTTATCCGCAATAACCGCGTTTTCATTATACAAGAAAATGATGTGCGGTTTTGCGAGCGTAAAtttt                                                                                                                 >  1:326951/1‑150 (MQ=255)
                                     tCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTg                                                                                                                >  2:157540/1‑150 (MQ=255)
                                       tGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                                                                                                              >  2:3285322/1‑150 (MQ=255)
                                          cGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCAccc                                                                                                           <  1:667356/150‑1 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                          >  1:4227031/1‑150 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                          >  1:979080/1‑150 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                          <  1:2053594/150‑1 (MQ=255)
                                            aaCATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCgg                                                                                                         >  2:1593407/1‑150 (MQ=255)
                                               aTCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTa                                                                                                      <  2:1400728/150‑1 (MQ=255)
                                               aTCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTa                                                                                                      >  1:1422571/1‑150 (MQ=255)
                                               aTCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTa                                                                                                      <  2:1888457/150‑1 (MQ=255)
                                               aTCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTa                                                                                                      <  1:2878603/150‑1 (MQ=255)
                                                tCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaa                                                                                                     >  1:4590924/1‑150 (MQ=255)
                                                 cGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaaa                                                                                                    <  2:2883931/150‑1 (MQ=255)
                                                 cGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaaa                                                                                                    >  2:3427372/1‑150 (MQ=255)
                                                  ggCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAac                                                                                                   >  2:4606265/1‑150 (MQ=255)
                                                  ggCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAac                                                                                                   >  1:3784424/1‑150 (MQ=255)
                                                      tGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACaa                                                                                               >  2:1150160/1‑150 (MQ=255)
                                                       gCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAt                                                                                              <  1:4494163/150‑1 (MQ=255)
                                                       gCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAt                                                                                              >  2:607392/1‑150 (MQ=255)
                                                        cAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAtt                                                                                             <  1:2345268/150‑1 (MQ=255)
                                                            atatCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGc                                                                                         <  2:4530669/150‑1 (MQ=255)
                                                              atCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCAt                                                                                       >  1:474789/1‑150 (MQ=255)
                                                                cATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATag                                                                                     >  1:3581825/1‑150 (MQ=255)
                                                                  ttGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATagag                                                                                   >  1:2435428/1‑150 (MQ=255)
                                                                     ttCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGtt                                                                                <  1:1259850/150‑1 (MQ=255)
                                                                     ttCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGtt                                                                                <  2:4105756/150‑1 (MQ=255)
                                                                      tctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTa                                                                               <  1:1724303/150‑1 (MQ=255)
                                                                       ctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTaa                                                                              >  2:4216950/1‑150 (MQ=255)
                                                                       ctcGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTaa                                                                              <  1:2837626/150‑1 (MQ=255)
                                                                         cGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAg                                                                            <  1:2205806/150‑1 (MQ=255)
                                                                         cGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAg                                                                            <  1:4026286/150‑1 (MQ=255)
                                                                          gATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAgg                                                                           <  1:680739/150‑1 (MQ=255)
                                                                           aTTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGa                                                                          >  2:3693579/1‑150 (MQ=255)
                                                                            ttttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAg                                                                         <  2:259738/150‑1 (MQ=255)
                                                                             tttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGt                                                                        <  2:2676349/150‑1 (MQ=255)
                                                                              ttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGtt                                                                       <  2:4352793/150‑1 (MQ=255)
                                                                                 cTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAAt                                                                    >  1:3999163/1‑150 (MQ=255)
                                                                                   ttCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTa                                                                  >  1:2704582/1‑150 (MQ=255)
                                                                                        gctgctGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGAt                                                             >  1:1058867/1‑150 (MQ=255)
                                                                                           gctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGtt                                                          <  2:2121371/150‑1 (MQ=255)
                                                                                           gctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGtt                                                          <  1:2798488/150‑1 (MQ=255)
                                                                                            ctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTa                                                         <  1:3238893/150‑1 (MQ=255)
                                                                                             tgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAc                                                        >  2:2786888/1‑150 (MQ=255)
                                                                                             tgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAc                                                        <  1:222934/150‑1 (MQ=255)
                                                                                             tgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAc                                                        >  1:862575/1‑150 (MQ=255)
                                                                                             tgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTAc                                                        <  1:558280/150‑1 (MQ=255)
                                                                                                  cTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCaa                                                   <  1:2635421/150‑1 (MQ=255)
                                                                                                  cTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCaa                                                   <  1:1917410/150‑1 (MQ=255)
                                                                                                      ttACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATa                                               <  1:269643/150‑1 (MQ=255)
                                                                                                       tACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATAc                                              >  1:4179956/1‑150 (MQ=255)
                                                                                                          cGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGaa                                           >  1:1115031/1‑150 (MQ=255)
                                                                                                          cGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGaa                                           <  2:2859052/150‑1 (MQ=255)
                                                                                                           gCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAat                                          <  2:3189397/150‑1 (MQ=255)
                                                                                                           gCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAat                                          >  1:2148375/1‑150 (MQ=255)
                                                                                                           gCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAat                                          >  2:4409362/1‑150 (MQ=255)
                                                                                                            cAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAatt                                         >  1:4237230/1‑150 (MQ=255)
                                                                                                            cAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAatt                                         <  1:488220/150‑1 (MQ=255)
                                                                                                             aaTTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattg                                        >  2:2460766/1‑150 (MQ=255)
                                                                                                              attattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattga                                       >  1:1601215/1‑150 (MQ=255)
                                                                                                              attattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattga                                       <  2:3716999/150‑1 (MQ=255)
                                                                                                              attattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattga                                       <  1:3712691/150‑1 (MQ=255)
                                                                                                              attattGTTTTTATCCGATATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGATTGGCGAGCGTAAATTTTGCACCCGTTTAAACACAAATAAGCATAGAGGTTAAAGGAGTTAAATATGGATGTTACCGCAAAATACGAattga                                       >  1:3334873/1‑150 (MQ=255)
                                                                                                               ttattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgat                                      >  1:4080018/1‑150 (MQ=255)
                                                                                                                  ttGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTgg                                   <  2:2606071/150‑1 (MQ=255)
                                                                                                                       tttATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGa                              >  1:436509/1‑150 (MQ=255)
                                                                                                                         tATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATg                            >  2:1608124/1‑150 (MQ=255)
                                                                                                                          aTCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATgg                           >  1:3117775/1‑150 (MQ=255)
                                                                                                                             cGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcct                        <  2:2825214/150‑1 (MQ=255)
                                                                                                                              gCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGccta                       >  1:169395/1‑150 (MQ=255)
                                                                                                                                tATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatc                     >  1:1493296/1‑150 (MQ=255)
                                                                                                                                 aTTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcc                    >  2:815525/1‑150 (MQ=255)
                                                                                                                                 aTTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcc                    >  2:3404026/1‑150 (MQ=255)
                                                                                                                                  ttACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcct                   <  2:299214/150‑1 (MQ=255)
                                                                                                                                   tACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatccta                  >  2:2153128/1‑150 (MQ=255)
                                                                                                                                    aCCGCGTTTTCAGTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcctct                 >  2:735538/1‑148 (MQ=255)
                                                                                                                                      cgcgTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCtatcctatcc               >  2:2887307/1‑150 (MQ=255)
                                                                                                                                       gcgTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCg              >  2:854368/1‑150 (MQ=255)
                                                                                                                                          ttttCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCca           <  1:799784/150‑1 (MQ=255)
                                                                                                                                            ttCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCcaca         >  1:1169883/1‑150 (MQ=255)
                                                                                                                                               attattCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGtt      >  2:4315596/1‑150 (MQ=255)
                                                                                                                                                 tattCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGGTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATAGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTa    >  2:556030/1‑150 (MQ=255)
                                                                                                                                                  attCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTAt   <  2:474789/150‑1 (MQ=255)
                                                                                                                                                  attCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTAt   <  2:2787054/150‑1 (MQ=255)
                                                                                                                                                  attCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTAt   >  2:2759348/1‑150 (MQ=255)
                                                                                                                                                   ttCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATc  <  1:586784/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATC  >  CP009273/1767814‑1768110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A