Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,767,963 C→A 41.9% P399T (CCT→ACT)  ydiN → MFS transporter superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,767,9630CA41.9% 148.2 / 200.0 160P399T (CCT→ACT) ydiNMFS transporter superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (54/39);  new base A (33/34);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 161 total aligned reads displayed.

GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATCG  >  CP009273/1767814‑1768111
                                                                                                                                                     |                                                                                                                                                    
     gCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGaa                                                                                                                                                 >  2:3863735/1‑150 (MQ=255)
     gCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGaa                                                                                                                                                 >  2:1455741/1‑150 (MQ=255)
     gCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGaa                                                                                                                                                 <  2:1014883/150‑1 (MQ=255)
       tAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTAATGaaaa                                                                                                                                               >  2:1434680/1‑150 (MQ=255)
        aaCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAat                                                                                                                                              <  1:3196717/150‑1 (MQ=255)
        aaCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAat                                                                                                                                              <  1:2696151/150‑1 (MQ=255)
        aaCTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAat                                                                                                                                              <  2:662759/150‑1 (MQ=255)
          cTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAatga                                                                                                                                            >  2:3380514/1‑150 (MQ=255)
             tGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGAtgt                                                                                                                                         <  2:611957/150‑1 (MQ=255)
               ttATTCCACTGATGACCGGTGATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGc                                                                                                                                       <  2:2230802/150‑1 (MQ=255)
                tATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCg                                                                                                                                      <  1:500104/150‑1 (MQ=255)
                 aTTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCgg                                                                                                                                     >  2:2221560/1‑150 (MQ=255)
                 aTTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCgg                                                                                                                                     >  1:1031825/1‑150 (MQ=255)
                 aTTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCgg                                                                                                                                     >  1:3433537/1‑150 (MQ=255)
                  ttCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGt                                                                                                                                    <  1:3730299/150‑1 (MQ=255)
                      aCTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTgg                                                                                                                                >  2:3688516/1‑150 (MQ=255)
                      aCTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTgg                                                                                                                                >  1:1920581/1‑150 (MQ=255)
                        tGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGAGATGCTGGCCCTGATTACCGCAATTATTGTTTTTATCAGATATTACCGCTTTTTCATTATTAATGAAAATGATGTGCGGTTTGGCg                                                                                                                              >  1:725005/1‑150 (MQ=255)
                          aTTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAg                                                                                                                            >  2:4507113/1‑150 (MQ=255)
                            tACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCg                                                                                                                          >  1:42796/1‑150 (MQ=255)
                            tACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCg                                                                                                                          >  1:42787/1‑150 (MQ=255)
                             aCCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGt                                                                                                                         >  1:235841/1‑150 (MQ=255)
                              ccGGTTATCTGTCGAACATCGGCCTGCAATCTATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTa                                                                                                                        <  2:431972/150‑1 (MQ=255)
                                ggTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTaaa                                                                                                                      >  1:4423512/1‑150 (MQ=255)
                                   gaTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAAttt                                                                                                                   <  2:4477075/149‑1 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtttt                                                                                                                  <  2:1285639/150‑1 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtttt                                                                                                                  <  1:774290/150‑1 (MQ=255)
                                    aTCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAAtttt                                                                                                                  <  2:3520763/150‑1 (MQ=255)
                                     tCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTg                                                                                                                 <  2:1350471/150‑1 (MQ=255)
                                       tGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                                                                                                               >  2:3066742/1‑150 (MQ=255)
                                       tGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCa                                                                                                               >  2:1088182/1‑150 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                           >  2:3803161/1‑150 (MQ=255)
                                           gAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCg                                                                                                           <  2:2954521/150‑1 (MQ=255)
                                             aCATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGt                                                                                                         <  1:3484715/150‑1 (MQ=255)
                                              cATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGtt                                                                                                        >  2:1934245/1‑150 (MQ=255)
                                                tCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTaa                                                                                                      <  1:2893988/150‑1 (MQ=255)
                                                  ggCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAac                                                                                                    <  1:2126781/150‑1 (MQ=255)
                                                  ggCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAac                                                                                                    <  1:2128232/150‑1 (MQ=255)
                                                  ggCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAac                                                                                                    <  1:4730177/150‑1 (MQ=255)
                                                       gCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAt                                                                                               <  2:1805981/150‑1 (MQ=255)
                                                       gCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAAt                                                                                               >  1:3625898/1‑150 (MQ=255)
                                                         aaTATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTa                                                                                             >  2:4346919/1‑150 (MQ=255)
                                                         aaTATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTa                                                                                             <  2:3572734/150‑1 (MQ=255)
                                                              atCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCAt                                                                                        <  1:2454673/150‑1 (MQ=255)
                                                              atCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCAt                                                                                        >  1:187849/1‑150 (MQ=255)
                                                              atCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCAt                                                                                        <  1:592958/150‑1 (MQ=255)
                                                              atCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCAt                                                                                        <  1:4046338/150‑1 (MQ=255)
                                                                cATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATag                                                                                      <  2:1721971/150‑1 (MQ=255)
                                                                 aTTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATaga                                                                                     <  1:933651/150‑1 (MQ=255)
                                                                   tGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAgg                                                                                   >  2:1633820/1‑150 (MQ=255)
                                                                    gTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGt                                                                                  <  1:1020041/150‑1 (MQ=255)
                                                                    gTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGt                                                                                  <  1:1271965/150‑1 (MQ=255)
                                                                     ttCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGtt                                                                                 <  1:1433973/150‑1 (MQ=255)
                                                                     ttCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGtt                                                                                 <  2:2121478/150‑1 (MQ=255)
                                                                     ttCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGtt                                                                                 <  2:4523276/150‑1 (MQ=255)
                                                                          gagtttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAgg                                                                            >  1:1525584/4‑150 (MQ=255)
                                                                          gATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAgg                                                                            >  1:3223455/1‑150 (MQ=255)
                                                                          gATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAgg                                                                            <  2:2818040/150‑1 (MQ=255)
                                                                           aTTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGa                                                                           <  2:2877815/150‑1 (MQ=255)
                                                                           aTTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGa                                                                           <  2:2644270/150‑1 (MQ=255)
                                                                           aTTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGa                                                                           >  2:2507556/1‑150 (MQ=255)
                                                                           aTTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGa                                                                           <  2:4089317/150‑1 (MQ=255)
                                                                             tttACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGt                                                                         >  1:3853239/1‑150 (MQ=255)
                                                                                  tttCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAAtt                                                                    <  1:1140984/150‑1 (MQ=255)
                                                                                   ttCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTa                                                                   <  1:3136411/150‑1 (MQ=255)
                                                                                   ttCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTa                                                                   <  1:3136407/150‑1 (MQ=255)
                                                                                      gcgcTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATgg                                                                <  1:2231059/150‑1 (MQ=255)
                                                                                          tgctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGt                                                            >  1:4280636/1‑150 (MQ=255)
                                                                                           gctgGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGtt                                                           <  1:1186854/150‑1 (MQ=255)
                                                                                               gCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCg                                                       >  1:3450672/1‑150 (MQ=255)
                                                                                                cccTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGc                                                      >  2:3143684/1‑150 (MQ=255)
                                                                                                  cTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCaa                                                    >  1:3777126/1‑150 (MQ=255)
                                                                                                       tACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATAc                                               <  2:42796/150‑1 (MQ=255)
                                                                                                        aCCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACg                                              <  1:3380514/150‑1 (MQ=255)
                                                                                                           gCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAat                                           <  1:2133292/150‑1 (MQ=255)
                                                                                                             aaTTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattg                                         <  1:2203593/150‑1 (MQ=255)
                                                                                                              attattGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattga                                        >  1:495197/1‑150 (MQ=255)
                                                                                                               ttattGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgat                                       >  2:1276514/1‑150 (MQ=255)
                                                                                                                tattGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgatt                                      >  2:3472496/1‑150 (MQ=255)
                                                                                                                 attGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgattg                                     >  2:3372227/1‑150 (MQ=255)
                                                                                                                 attGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAattgattg                                     >  2:3370977/1‑150 (MQ=255)
                                                                                                                  ttGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTgg                                    >  1:1134530/1‑150 (MQ=255)
                                                                                                                  ttGTTTTTATCCGCTATTACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTgg                                    >  1:1134622/1‑150 (MQ=255)
                                                                                                                    gTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGt                                  >  2:150164/1‑150 (MQ=255)
                                                                                                                             cGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcct                         >  1:1575745/1‑150 (MQ=255)
                                                                                                                             cGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcct                         >  1:2203043/1‑150 (MQ=255)
                                                                                                                               cTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctat                       <  2:4188716/150‑1 (MQ=255)
                                                                                                                                  ttACCGCGTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatcct                    >  1:2569353/1‑150 (MQ=255)
                                                                                                                                   tACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATTGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGcctatccta                   >  1:3237773/1‑150 (MQ=255)
                                                                                                                                     ccGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCctatcctatc                 >  1:886850/1‑150 (MQ=255)
                                                                                                                                      cgcgTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCtatcctatcc                >  2:1014167/1‑150 (MQ=255)
                                                                                                                                        cgTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGc              >  1:3990515/1‑150 (MQ=255)
                                                                                                                                         gTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGcc             >  2:3886238/1‑150 (MQ=255)
                                                                                                                                         gTTTTCATTATTACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGcc             >  2:4038913/1‑150 (MQ=255)
                                                                                                                                              cATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGt        >  1:95930/1‑150 (MQ=255)
                                                                                                                                              cATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGt        >  1:85393/1‑150 (MQ=255)
                                                                                                                                               attattCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGtt       >  2:3639240/1‑150 (MQ=255)
                                                                                                                                                 tattACTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTa     <  1:3417186/150‑1 (MQ=255)
                                                                                                                                                   ttCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATc   >  1:3435569/1‑150 (MQ=255)
                                                                                                                                                   ttCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATc   >  1:3964379/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GACTGGCTAACTTTGTTATTCCACTGATTACCGGTTATCTGTCGAACATCGGCCTGCAATATATCATTGTTCTCGATTTTACTTTCGCGCTGCTGGCCCTGATTACCGCAATTATTGTTTTTATCCGCTATTACCGCGTTTTCATTATTCCTGAAAATGATGTGCGGTTTGGCGAGCGTAAATTTTGCACCCGGTTAAACACAATTAAGCATAGAGGTTAAAGGAGTTAATTATGGATGTTACCGCAAAATACGAATTGATTGGGTTGATGGCCTATCCTATCCGCCACAGTTTATCG  >  CP009273/1767814‑1768111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A