Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,257,209 C→A 5.4% E390* (GAA→TAA)  tdcC ← L‑threonine/L‑serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,257,2090CA 5.4% 1300.2 / 30.4 324E390* (GAA→TAA) tdcCL‑threonine/L‑serine transporter
Reads supporting (aligned to +/- strand):  ref base C (139/167);  new base A (4/14);  total (143/181)
Fisher's exact test for biased strand distribution p-value = 8.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

TACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTT  >  CP009273/3257060‑3257358
                                                                                                                                                     |                                                                                                                                                     
  cGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCaa                                                                                                                                                     >  2:774842/1‑150 (MQ=255)
   gATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAAt                                                                                                                                                    <  1:288777/150‑1 (MQ=255)
   gATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAAt                                                                                                                                                    <  2:5044785/150‑1 (MQ=255)
     tGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCa                                                                                                                                                  >  2:126437/1‑150 (MQ=255)
       ttCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAgg                                                                                                                                                <  1:1002134/150‑1 (MQ=255)
       ttCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGAGGCGATACTCGGTGCGCCCATGGCTTCAATCAgg                                                                                                                                                >  1:438068/1‑150 (MQ=255)
            gATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAg                                                                                                                                           <  1:2275652/150‑1 (MQ=255)
             aTGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCACGGCTTAAATCAGGTCAAgg                                                                                                                                          <  1:1279877/150‑1 (MQ=255)
              tGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGa                                                                                                                                         <  1:810582/150‑1 (MQ=255)
               ggTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGAt                                                                                                                                        <  1:887715/150‑1 (MQ=255)
               ggTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGAt                                                                                                                                        <  2:1685327/150‑1 (MQ=255)
                  cagcagACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGtt                                                                                                                                     >  2:1313889/1‑150 (MQ=255)
                   agcagACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTc                                                                                                                                    <  1:3216182/150‑1 (MQ=255)
                    gcagACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCg                                                                                                                                   <  1:190056/150‑1 (MQ=255)
                    gcagACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCg                                                                                                                                   <  1:119296/150‑1 (MQ=255)
                    gcagACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCg                                                                                                                                   <  1:2787535/150‑1 (MQ=255)
                         ccAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTg                                                                                                                              <  1:1420036/150‑1 (MQ=255)
                          cAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTgg                                                                                                                             >  1:4935741/1‑150 (MQ=255)
                           aaTCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGc                                                                                                                            >  2:4963513/1‑150 (MQ=255)
                              cACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTa                                                                                                                         >  1:2841469/1‑150 (MQ=255)
                               aCGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAg                                                                                                                        <  2:3981324/150‑1 (MQ=255)
                                  gTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGca                                                                                                                     >  1:2547638/1‑150 (MQ=255)
                                    aacaaacaCGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGcaac                                                                                                                   >  1:2025764/1‑150 (MQ=255)
                                     acaaacaCGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGcaaca                                                                                                                  <  1:1775627/150‑1 (MQ=255)
                                        aacaCGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAAcc                                                                                                               >  2:2019247/1‑150 (MQ=255)
                                         acacGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAAccc                                                                                                              <  1:16234/150‑1 (MQ=255)
                                          cacGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCa                                                                                                             >  2:4994881/1‑150 (MQ=255)
                                          cacGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCa                                                                                                             >  2:1035193/1‑150 (MQ=255)
                                           acGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAg                                                                                                            <  2:2452705/150‑1 (MQ=255)
                                             gTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGt                                                                                                          >  2:704865/1‑150 (MQ=255)
                                              ttATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTg                                                                                                         >  2:4682061/1‑150 (MQ=255)
                                              ttATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTg                                                                                                         <  1:237696/150‑1 (MQ=255)
                                              ttATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTAAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTg                                                                                                         <  1:2068764/150‑1 (MQ=255)
                                               tATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTgg                                                                                                        <  2:2581277/150‑1 (MQ=255)
                                               tATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTgg                                                                                                        >  1:1465457/1‑150 (MQ=255)
                                               tATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTgg                                                                                                        <  2:5125151/150‑1 (MQ=255)
                                                aTCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGa                                                                                                       <  1:3282127/150‑1 (MQ=255)
                                                     gacgacCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCa                                                                                                  >  2:3431468/1‑150 (MQ=255)
                                                      acgacCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCat                                                                                                 <  2:3894064/150‑1 (MQ=255)
                                                       cgacCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCatg                                                                                                >  2:1608241/1‑150 (MQ=255)
                                                       cgacCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCatg                                                                                                <  1:4496631/150‑1 (MQ=255)
                                                       cgacCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCatg                                                                                                <  1:951476/150‑1 (MQ=255)
                                                         acCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCatgat                                                                                              <  1:733990/150‑1 (MQ=255)
                                                             cGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAg                                                                                          <  2:2814280/150‑1 (MQ=255)
                                                              ggTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGa                                                                                         >  2:3818706/1‑150 (MQ=255)
                                                               gTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGAt                                                                                        >  2:2267571/1‑150 (MQ=255)
                                                               gTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGAt                                                                                        <  1:4829371/150‑1 (MQ=255)
                                                               gTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGAt                                                                                        >  2:1884861/1‑150 (MQ=255)
                                                                 aTTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCa                                                                                      <  1:157013/150‑1 (MQ=255)
                                                                 aTTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCa                                                                                      >  1:1952070/1‑150 (MQ=255)
                                                                  tttCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTAAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCAt                                                                                     <  2:3879740/150‑1 (MQ=255)
                                                                       ccAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGa                                                                                >  2:5014590/1‑150 (MQ=255)
                                                                       ccAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGa                                                                                >  2:1724581/1‑150 (MQ=255)
                                                                        cAGAGACGGCGCTTTACGGATGGCATACATCGGCAACATGCACAGCAGGGATTAGATAATCTGTGCGCCCATGGCTTCAATCATGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATTATGAAGATCATGCTGag                                                                               >  1:3142213/1‑149 (MQ=255)
                                                                         agagACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATa                                                                              >  1:3574014/1‑150 (MQ=255)
                                                                                gcgcTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGATTAAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCa                                                                       <  1:4771886/150‑1 (MQ=255)
                                                                                 cgcTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAg                                                                      >  1:1089074/1‑150 (MQ=255)
                                                                                  gcTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGt                                                                     >  2:2917702/1‑150 (MQ=255)
                                                                                  gcTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGt                                                                     >  2:2732465/1‑150 (MQ=255)
                                                                                  gcTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCAGGCTGATAGTGTTCAGt                                                                     >  1:3482288/1‑150 (MQ=255)
                                                                                   cTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGtt                                                                    >  1:2842693/1‑150 (MQ=255)
                                                                                    tttACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGttt                                                                   <  1:3776378/150‑1 (MQ=255)
                                                                                    tttACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGttt                                                                   <  1:141848/150‑1 (MQ=255)
                                                                                    tttACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGttt                                                                   <  1:2197306/150‑1 (MQ=255)
                                                                                    tttACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTAAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGttt                                                                   <  2:866862/150‑1 (MQ=255)
                                                                                    tttACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGAGAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGGTGGCGTAGGCAACAACCCAGGTGGAGCCCAGGAGGAAGATCATGCTGAGCGTGTTCAGttt                                                                   >  2:4469746/1‑150 (MQ=255)
                                                                                      tACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTAc                                                                 <  2:4185697/150‑1 (MQ=255)
                                                                                        cGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTAccc                                                               >  1:4602700/1‑150 (MQ=255)
                                                                                         ggATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCa                                                              >  2:2485138/1‑150 (MQ=255)
                                                                                          gATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAg                                                             >  1:4986627/1‑150 (MQ=255)
                                                                                                   cATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACttt                                                    <  2:3100334/150‑1 (MQ=255)
                                                                                                       ggCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGtt                                                >  1:3882527/1‑150 (MQ=255)
                                                                                                          aaCAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTg                                             >  2:462092/1‑150 (MQ=255)
                                                                                                            cAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTc                                           <  1:4792681/150‑1 (MQ=255)
                                                                                                             aGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCg                                          <  1:325430/150‑1 (MQ=255)
                                                                                                               gCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGcc                                        >  1:4233663/1‑150 (MQ=255)
                                                                                                                  cagcagGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCttt                                     >  1:3912355/1‑150 (MQ=255)
                                                                                                                    gcagGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTtat                                   >  2:1867959/1‑150 (MQ=255)
                                                                                                                            gCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAAc                           <  2:59539/150‑1 (MQ=255)
                                                                                                                              gATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACtt                         >  2:192550/1‑150 (MQ=255)
                                                                                                                                tAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCa                       <  2:3243246/150‑1 (MQ=255)
                                                                                                                                tAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCa                       <  1:2470584/150‑1 (MQ=255)
                                                                                                                                 aaTCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAg                      <  1:3971772/150‑1 (MQ=255)
                                                                                                                                  aTCGGTGCGCCCATGGATTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAgg                     <  1:3062182/150‑1 (MQ=255)
                                                                                                                                   tCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGa                    <  1:1035193/150‑1 (MQ=255)
                                                                                                                                    cGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACAGTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGAc                   >  1:33561/1‑150 (MQ=255)
                                                                                                                                    cGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACAGTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGAc                   >  1:33949/1‑150 (MQ=255)
                                                                                                                                     ggTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGAcc                  <  1:4004319/150‑1 (MQ=255)
                                                                                                                                     ggTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGAcc                  >  1:2594210/1‑150 (MQ=255)
                                                                                                                                      gTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCa                 >  2:4548750/1‑150 (MQ=255)
                                                                                                                                         cgcCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACCCTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGc              >  1:2117111/1‑150 (MQ=255)
                                                                                                                                         cgcCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGc              >  1:1524578/1‑150 (MQ=255)
                                                                                                                                           cccATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCa            <  2:488153/150‑1 (MQ=255)
                                                                                                                                           cccATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCa            >  2:1387794/1‑150 (MQ=255)
                                                                                                                                             cATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACACAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTAAGGACCAGGCCAtt          >  1:1687095/1‑150 (MQ=255)
                                                                                                                                             cATGGCTTAAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCAtt          >  2:3574472/1‑150 (MQ=255)
                                                                                                                                                 gCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGa      >  2:319194/1‑150 (MQ=255)
                                                                                                                                                    tCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCt   <  2:2515666/150‑1 (MQ=255)
                                                                                                                                                    tCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCt   <  1:706456/150‑1 (MQ=255)
                                                                                                                                                     cAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCtt  <  1:13568/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACGATGTTCAGGATGGTCAGCAGACCAATCACGGTAACAAACACGTTATCCAGACGACCACGGTATTTCGCCAGAGACGGCGCTTTACGGATGGCATACATCGGCAACAGGCACAGCAGGGATGCGATAATCGGTGCGCCCATGGCTTCAATCAGGTCAAGGATGTTCGGGTTGGCGTAGGCAACAACCCAGGTGGAGCCCATGATGAAGATCATGCTGATAGTGTTCAGTTTACCCAGCGACACTTTAGTTTTGTCGCCTTTATAACCAAACTTCAGGACCAGGCCATTCAGACCTT  >  CP009273/3257060‑3257358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A