Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,513,424 A→T 49.4% V213V (GTT→GTA)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,513,4240AT49.4% 20.6 / inf 358V213V (GTT→GTA) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base A (92/89);  new base T (88/89);  total (180/178)
Fisher's exact test for biased strand distribution p-value = 9.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

GGACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAA  >  CP009273/3513275‑3513573
                                                                                                                                                     |                                                                                                                                                     
ggACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCa                                                                                                                                                       >  1:4695320/1‑150 (MQ=255)
 gACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTCCGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCaa                                                                                                                                                      >  2:4013699/1‑150 (MQ=255)
  aCTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTAc                                                                                                                                                     >  1:4923554/1‑150 (MQ=255)
   cTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACg                                                                                                                                                    >  2:287383/1‑150 (MQ=255)
   cTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACg                                                                                                                                                    <  1:2788626/150‑1 (MQ=255)
    tGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGc                                                                                                                                                   >  2:2628218/1‑150 (MQ=255)
     ggCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGcc                                                                                                                                                  >  2:2100560/1‑150 (MQ=255)
          taataTCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAg                                                                                                                                             >  1:2671590/1‑150 (MQ=255)
            atatCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACCCCACCAGCg                                                                                                                                           >  2:4463098/1‑150 (MQ=255)
             tatCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCgt                                                                                                                                          >  1:3505827/1‑150 (MQ=255)
             tatCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCgt                                                                                                                                          <  1:4342688/150‑1 (MQ=255)
               tCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCgtgt                                                                                                                                        >  2:5140302/1‑150 (MQ=255)
                 cAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTg                                                                                                                                      <  2:1974861/150‑1 (MQ=255)
                 cAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTg                                                                                                                                      <  2:2792005/150‑1 (MQ=255)
                  aGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTgc                                                                                                                                     >  1:1298324/1‑150 (MQ=255)
                    ctgctgTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTgcgc                                                                                                                                   >  1:4810412/1‑150 (MQ=255)
                      gctgTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCAt                                                                                                                                 <  1:4028874/150‑1 (MQ=255)
                              tGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGc                                                                                                                         >  1:2483115/1‑150 (MQ=255)
                              tGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGc                                                                                                                         >  1:1513787/1‑150 (MQ=255)
                               gTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCg                                                                                                                        >  1:1524106/1‑150 (MQ=255)
                                ttCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGt                                                                                                                       >  1:3452601/1‑150 (MQ=255)
                                 tCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTc                                                                                                                      <  2:4985836/150‑1 (MQ=255)
                                       gcgGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTc                                                                                                                <  2:22508/150‑1 (MQ=255)
                                        cgGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCa                                                                                                               >  1:2385013/1‑150 (MQ=255)
                                         ggAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCAc                                                                                                              >  2:1338960/1‑150 (MQ=255)
                                         ggAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCAc                                                                                                              >  1:3003642/1‑150 (MQ=255)
                                             aGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCt                                                                                                          <  1:987964/150‑1 (MQ=255)
                                             aGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCt                                                                                                          <  2:489097/150‑1 (MQ=255)
                                              gCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTa                                                                                                         <  2:369374/150‑1 (MQ=255)
                                               cTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTaa                                                                                                        >  2:1860847/1‑150 (MQ=255)
                                               cTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTaa                                                                                                        <  1:1919392/150‑1 (MQ=255)
                                                    gATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCAc                                                                                                   <  2:576650/150‑1 (MQ=255)
                                                      tCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGc                                                                                                 >  1:3823913/1‑150 (MQ=255)
                                                          gCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAAc                                                                                             >  1:1795373/1‑150 (MQ=255)
                                                          gCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAAc                                                                                             <  2:756318/150‑1 (MQ=255)
                                                           cAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACt                                                                                            >  1:4068805/1‑150 (MQ=255)
                                                            aaGCGTCCGTTGATGCGCCCAATGTTAAATCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtt                                                                                           >  2:1092436/1‑150 (MQ=255)
                                                            aaGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtt                                                                                           >  1:3722383/1‑150 (MQ=255)
                                                            aaGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtt                                                                                           <  1:871100/150‑1 (MQ=255)
                                                             aGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACttt                                                                                          >  2:3238665/1‑150 (MQ=255)
                                                                gTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtttttt                                                                                       >  2:4239850/1‑150 (MQ=255)
                                                                gTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtttttt                                                                                       >  2:3951477/1‑150 (MQ=255)
                                                                    gTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCAtt                                                                                   <  1:2611802/150‑1 (MQ=255)
                                                                     ttGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTa                                                                                  >  1:960106/1‑150 (MQ=255)
                                                                      tGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTaa                                                                                 <  2:1838397/150‑1 (MQ=255)
                                                                      tGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTaa                                                                                 >  2:1604360/1‑150 (MQ=255)
                                                                         tGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATgg                                                                              <  2:2442028/150‑1 (MQ=255)
                                                                          gcgcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGt                                                                             <  1:752832/150‑1 (MQ=255)
                                                                          gcgcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGt                                                                             >  1:1057780/1‑150 (MQ=255)
                                                                          gcgcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGt                                                                             <  1:1667659/150‑1 (MQ=255)
                                                                            gcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGa                                                                           >  1:138257/1‑150 (MQ=255)
                                                                            gcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGa                                                                           >  1:114605/1‑150 (MQ=255)
                                                                                  tGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAatat                                                                     >  2:1729008/1‑150 (MQ=255)
                                                                                  tGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCAAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATATCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAatat                                                                     >  2:4290305/1‑150 (MQ=255)
                                                                                    ttAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgc                                                                   >  2:969868/1‑150 (MQ=255)
                                                                                    ttAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgc                                                                   <  1:2306211/150‑1 (MQ=255)
                                                                                     tAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgcg                                                                  >  2:3570576/1‑150 (MQ=255)
                                                                                       aaaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGcc                                                                <  2:4151307/150‑1 (MQ=255)
                                                                                        aaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCg                                                               <  1:2117020/150‑1 (MQ=255)
                                                                                         aCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGa                                                              >  2:4839039/1‑150 (MQ=255)
                                                                                               aTGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTc                                                        >  2:529759/1‑150 (MQ=255)
                                                                                               aTGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTc                                                        <  2:1496706/150‑1 (MQ=255)
                                                                                                    ttGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCt                                                   <  1:645425/150‑1 (MQ=255)
                                                                                                     tGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTg                                                  >  1:3598357/1‑150 (MQ=255)
                                                                                                     tGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTg                                                  >  2:4075750/1‑150 (MQ=255)
                                                                                                     tGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTg                                                  <  1:4717235/150‑1 (MQ=255)
                                                                                                     tGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTg                                                  >  2:3072695/1‑150 (MQ=255)
                                                                                                      gTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGc                                                 >  1:593291/1‑150 (MQ=255)
                                                                                                        cGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTATTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGccc                                               <  2:2768482/150‑1 (MQ=255)
                                                                                                        cGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGccc                                               <  1:3216658/150‑1 (MQ=255)
                                                                                                          ccGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGa                                             <  2:3790110/150‑1 (MQ=255)
                                                                                                          ccGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGa                                             <  1:79490/150‑1 (MQ=255)
                                                                                                           cGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAc                                            >  2:1896582/1‑150 (MQ=255)
                                                                                                           cGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAc                                            >  1:3364322/1‑150 (MQ=255)
                                                                                                           cGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCAGTAATGGTGACCATATGCGCCGACAGCTCAACCTGCCCGAc                                            >  1:1858592/1‑150 (MQ=255)
                                                                                                            gTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAcc                                           <  2:1669977/150‑1 (MQ=255)
                                                                                                            gTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAcc                                           >  1:2895834/1‑150 (MQ=255)
                                                                                                               gCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGc                                        <  1:3282992/150‑1 (MQ=255)
                                                                                                               gCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGc                                        >  2:2917548/1‑150 (MQ=255)
                                                                                                                cTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCa                                       <  2:2483115/150‑1 (MQ=255)
                                                                                                                 tACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAg                                      >  2:2383269/1‑150 (MQ=255)
                                                                                                                  aCGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGa                                     >  1:1645362/1‑150 (MQ=255)
                                                                                                                  aCGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGa                                     <  1:19994/150‑1 (MQ=255)
                                                                                                                    ggAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATc                                   >  1:3101198/1‑150 (MQ=255)
                                                                                                                    ggAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATc                                   <  2:2558149/150‑1 (MQ=255)
                                                                                                                         ggTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAttt                              <  1:2252925/150‑1 (MQ=255)
                                                                                                                         ggTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAttt                              <  2:2032498/150‑1 (MQ=255)
                                                                                                                          gTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTg                             >  2:4364912/1‑150 (MQ=255)
                                                                                                                             gCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTgcgc                          <  2:4571714/150‑1 (MQ=255)
                                                                                                                              cTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCt                         <  2:4133330/150‑1 (MQ=255)
                                                                                                                               tACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTa                        <  2:1520923/150‑1 (MQ=255)
                                                                                                                                aCCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTAc                       >  1:219036/1‑150 (MQ=255)
                                                                                                                                   aaGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCa                    <  1:4165953/150‑1 (MQ=255)
                                                                                                                                   aaGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCa                    <  1:1604360/150‑1 (MQ=255)
                                                                                                                                      cGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTg                 <  2:1524106/150‑1 (MQ=255)
                                                                                                                                       gtggtgACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGt                >  2:1151438/1‑150 (MQ=255)
                                                                                                                                            gACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAg           >  1:1085008/1‑150 (MQ=255)
                                                                                                                                             aCTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAgc          >  1:1755406/1‑150 (MQ=255)
                                                                                                                                                  cccTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCt     <  2:832782/150‑1 (MQ=255)
                                                                                                                                                     aaCGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTaa  <  2:3446794/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAA  >  CP009273/3513275‑3513573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A