Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,513,424 A→T 5.4% V213V (GTT→GTA)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,513,4240AT 5.4% 906.6 / 31.1 261V213V (GTT→GTA) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base A (131/115);  new base T (10/4);  total (142/119)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 265 total aligned reads displayed.

GGACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAA  >  CP009273/3513275‑3513573
                                                                                                                                                     |                                                                                                                                                     
   cTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACg                                                                                                                                                    <  2:3724475/150‑1 (MQ=255)
    tGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGc                                                                                                                                                   >  1:712254/1‑150 (MQ=255)
       cGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGccac                                                                                                                                                >  2:1924746/1‑150 (MQ=255)
                ccAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGtt                                                                                                                                       <  2:1421679/150‑1 (MQ=255)
                 cAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTg                                                                                                                                      >  2:1555713/1‑150 (MQ=255)
                  aGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTgc                                                                                                                                     >  2:4441758/1‑150 (MQ=255)
                      gctgTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCAt                                                                                                                                 >  2:1605231/1‑150 (MQ=255)
                                ttCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGt                                                                                                                       >  2:3846635/1‑150 (MQ=255)
                                 tCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTc                                                                                                                      >  1:929224/1‑150 (MQ=255)
                                  cGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTcc                                                                                                                     >  2:911437/1‑150 (MQ=255)
                                  cGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTcc                                                                                                                     <  1:4332132/150‑1 (MQ=255)
                                  cGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTcc                                                                                                                     <  1:441637/150‑1 (MQ=255)
                                     gcgcGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCAtt                                                                                                                  <  2:120866/150‑1 (MQ=255)
                                        cgGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCa                                                                                                               >  1:3125937/1‑150 (MQ=255)
                                         ggAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCAc                                                                                                              >  1:3991454/1‑150 (MQ=255)
                                         ggAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCAc                                                                                                              >  1:3519490/1‑150 (MQ=255)
                                          gAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACg                                                                                                             >  2:917777/1‑150 (MQ=255)
                                           aaaGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGc                                                                                                            >  1:2442981/1‑150 (MQ=255)
                                           aaaGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGc                                                                                                            >  2:3314460/1‑150 (MQ=255)
                                           aaaGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGc                                                                                                            >  1:2228692/1‑150 (MQ=255)
                                             aGCTCAAGATCCAGCAAGCGTCCGTTGTTGCGCCCAATGTTAAAACCGACTTGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCt                                                                                                          <  1:3870799/150‑1 (MQ=255)
                                             aGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCt                                                                                                          <  2:327448/150‑1 (MQ=255)
                                             aGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCt                                                                                                          <  1:3614170/150‑1 (MQ=255)
                                              gCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTa                                                                                                         <  1:522850/150‑1 (MQ=255)
                                                tCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAAc                                                                                                       <  2:2313000/150‑1 (MQ=255)
                                                  aaGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTc                                                                                                     >  2:1469342/1‑150 (MQ=255)
                                                    gATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCAc                                                                                                   >  1:1693528/1‑150 (MQ=255)
                                                    gATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCAc                                                                                                   <  1:3929127/150‑1 (MQ=255)
                                                    gATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCAc                                                                                                   <  2:2110874/150‑1 (MQ=255)
                                                     aTCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACg                                                                                                  <  1:283337/150‑1 (MQ=255)
                                                     aTCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACg                                                                                                  <  2:4128831/150‑1 (MQ=255)
                                                      tCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGc                                                                                                 >  1:1002398/1‑150 (MQ=255)
                                                      tCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGc                                                                                                 >  1:1068243/1‑150 (MQ=255)
                                                        aaGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGATGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACATGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATATCACGCCTAACTCACGCaa                                                                                               >  2:2882528/2‑150 (MQ=255)
                                                            aaGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtt                                                                                           <  1:3282145/150‑1 (MQ=255)
                                                             aGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACttt                                                                                          >  1:2415415/1‑150 (MQ=255)
                                                               cGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTCACTCACGCAAACttttt                                                                                        >  1:3618740/1‑150 (MQ=255)
                                                               cGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACttttt                                                                                        >  2:1971052/1‑150 (MQ=255)
                                                               cGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACttttt                                                                                        >  2:4422173/1‑150 (MQ=255)
                                                                gTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtttttt                                                                                       >  2:944803/1‑150 (MQ=255)
                                                                gTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACtttttt                                                                                       >  1:996454/1‑150 (MQ=255)
                                                                 tCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTc                                                                                      <  2:3181991/150‑1 (MQ=255)
                                                                   cGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCAt                                                                                    >  1:1562014/1‑150 (MQ=255)
                                                                    gTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCAtt                                                                                   <  2:3125937/150‑1 (MQ=255)
                                                                     ttGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTa                                                                                  <  2:3477384/150‑1 (MQ=255)
                                                                      tGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTaa                                                                                 >  1:1042456/1‑150 (MQ=255)
                                                                      tGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTaa                                                                                 >  1:626119/1‑150 (MQ=255)
                                                                       gATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAAt                                                                                <  2:1741007/150‑1 (MQ=255)
                                                                        aTGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATg                                                                               <  1:220499/150‑1 (MQ=255)
                                                                            gcCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGa                                                                           >  1:4117665/1‑150 (MQ=255)
                                                                             cccAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGAc                                                                          >  2:1220303/1‑150 (MQ=255)
                                                                             cccAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGAc                                                                          <  2:929224/150‑1 (MQ=255)
                                                                             cccAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGAc                                                                          <  1:4470432/150‑1 (MQ=255)
                                                                             cccAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGAc                                                                          <  1:4140433/150‑1 (MQ=255)
                                                                              ccAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACa                                                                         >  1:1781834/1‑150 (MQ=255)
                                                                                aaTGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAat                                                                       <  2:2880920/150‑1 (MQ=255)
                                                                                     tAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgcg                                                                  <  1:866594/150‑1 (MQ=255)
                                                                                      aaaaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgcgc                                                                 >  2:1914685/1‑150 (MQ=255)
                                                                                      aaaaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATgcgc                                                                 <  1:2259679/150‑1 (MQ=255)
                                                                                       aaaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGcc                                                                >  2:4245500/1‑150 (MQ=255)
                                                                                        aaCCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCg                                                               >  1:4453681/1‑150 (MQ=255)
                                                                                          ccGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGAc                                                             <  2:2362240/150‑1 (MQ=255)
                                                                                            gACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAg                                                           <  2:3159428/150‑1 (MQ=255)
                                                                                              cATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCt                                                         >  1:3197792/1‑150 (MQ=255)
                                                                                              cATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCt                                                         >  1:2558065/1‑150 (MQ=255)
                                                                                              cATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCt                                                         >  1:3197884/1‑150 (MQ=255)
                                                                                               aTGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTc                                                        >  2:1694482/1‑150 (MQ=255)
                                                                                                    ttGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCt                                                   <  1:1943022/150‑1 (MQ=255)
                                                                                                     tGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTg                                                  <  2:1826002/150‑1 (MQ=255)
                                                                                                        cGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGccc                                               <  1:755406/150‑1 (MQ=255)
                                                                                                          ccGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGa                                             <  1:1755712/150‑1 (MQ=255)
                                                                                                           cGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAc                                            <  1:1838133/150‑1 (MQ=255)
                                                                                                           cGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAc                                            >  2:2208078/1‑150 (MQ=255)
                                                                                                            gTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCTACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGAcc                                           <  2:3974729/150‑1 (MQ=255)
                                                                                                             tCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCg                                          <  2:3534966/150‑1 (MQ=255)
                                                                                                              cGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCgg                                         >  2:1667312/1‑150 (MQ=255)
                                                                                                                 tACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAg                                      >  1:2313547/1‑150 (MQ=255)
                                                                                                                     gagaGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATcc                                  <  2:3684031/150‑1 (MQ=255)
                                                                                                                     gagaGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATcc                                  <  2:3037384/150‑1 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCCGATCCAtt                               >  1:2788206/1‑150 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAtt                               <  2:2418083/150‑1 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAtt                               >  2:2726294/1‑150 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAtt                               <  2:1828583/150‑1 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAtt                               >  2:1519546/1‑150 (MQ=255)
                                                                                                                        agGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTGTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCAtt                               >  1:1157895/1‑150 (MQ=255)
                                                                                                                          gTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTg                             >  1:665202/1‑150 (MQ=255)
                                                                                                                                   aaGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCa                    >  2:2255870/1‑150 (MQ=255)
                                                                                                                                   aaGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCa                    <  1:127902/150‑1 (MQ=255)
                                                                                                                                    aGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCAc                   >  1:3114294/1‑150 (MQ=255)
                                                                                                                                     gCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACt                  <  2:2588911/150‑1 (MQ=255)
                                                                                                                                     gCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACt                  <  2:4157610/150‑1 (MQ=255)
                                                                                                                                       gtggtgACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGt                >  1:346251/1‑150 (MQ=255)
                                                                                                                                        tggtgACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGtt               <  1:2058414/150‑1 (MQ=255)
                                                                                                                                        tggtgACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGtt               <  2:1987422/150‑1 (MQ=255)
                                                                                                                                        tggtgACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGtt               >  1:3640962/1‑150 (MQ=255)
                                                                                                                                               ttGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAgcgc        >  1:482632/1‑150 (MQ=255)
                                                                                                                                                  cccAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCt     >  2:2686906/1‑150 (MQ=255)
                                                                                                                                                  cccAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCt     >  2:2686289/1‑150 (MQ=255)
                                                                                                                                                    cAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTa   <  2:195042/150‑1 (MQ=255)
                                                                                                                                                     aaCGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTaa  <  2:1397775/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACTGGCGATAATATCCAGCTGCTGTTTTTGTTCGAGCGCGGAAAGCTCAAGATCCAGCAAGCGTCCGTTGATGCGCCCAATGTTAAAACCGACATGCGTTGTCGCCGTCGCTACGGAGAGGTCGCTACCAAGCGTGGTGACTTGCCCAACGCCACCAGCGTGTTGCGCATCGGCCAGCGTCCATTTCACGCCTAACTCACGCAAACTTTTTTCATTAATGGTGACAATATGCGCCGACAGCTCAACCTGCCCGACCGGCAGATCCATTTGCGCTACCCACTGTTCAAGCGCGCTTAA  >  CP009273/3513275‑3513573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A