Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F181 I0 R1	1262	323.1	10297870	97.5%	10040423	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,144,787	C→A	5.8%	intergenic (‑12/‑142)	argE ← / → argC	acetylornithine deacetylase/N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,144,787	0	C	A	5.8%	1263.3 / 31.5	321	intergenic (‑12/‑142)	argE/argC	acetylornithine deacetylase/N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base C (155/147); new base A (5/14); total (160/161)
Fisher's exact test for biased strand distribution p-value = 5.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

CAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAA  >  CP009273/4144638‑4144936

cAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACAcc                                                                                                                                                       <  1:1135623/150‑1 (MQ=255)

 aactCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACct                                                                                                                                                      <  2:1737662/147‑1 (MQ=255)

 aaaTCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACct                                                                                                                                                      <  1:4587671/150‑1 (MQ=255)

 aaaTCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGACTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACct                                                                                                                                                      <  1:1301090/150‑1 (MQ=255)

  aaTCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACctc                                                                                                                                                     <  1:1815466/150‑1 (MQ=255)

   aTCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACctct                                                                                                                                                    <  1:1722392/150‑1 (MQ=255)

       ttAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTc                                                                                                                                                <  1:4432928/150‑1 (MQ=255)

        tAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCa                                                                                                                                               <  2:790173/150‑1 (MQ=255)

         aaaCCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCAt                                                                                                                                              >  2:39769/1‑150 (MQ=255)

         aaaCCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCAt                                                                                                                                              <  1:4924259/150‑1 (MQ=255)

         aaaCCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCAt                                                                                                                                              <  1:3886255/150‑1 (MQ=255)

           aCCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGa                                                                                                                                            >  1:3693516/1‑150 (MQ=255)

             cAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATa                                                                                                                                          >  1:4831462/1‑150 (MQ=255)

              aGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAg                                                                                                                                         >  1:5062910/1‑150 (MQ=255)

              aGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAg                                                                                                                                         >  2:1068178/1‑150 (MQ=255)

               gTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGACTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGt                                                                                                                                        >  1:2062248/1‑150 (MQ=255)

                tCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGACTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTa                                                                                                                                       >  2:2367855/1‑150 (MQ=255)

                   gCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCa                                                                                                                                    >  2:2137085/1‑150 (MQ=255)

                   gCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCa                                                                                                                                    <  2:4851302/150‑1 (MQ=255)

                   gCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCa                                                                                                                                    >  2:1843683/1‑150 (MQ=255)

                   gCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCa                                                                                                                                    <  2:3326626/150‑1 (MQ=255)

                     cagcagAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAat                                                                                                                                  <  1:2765227/150‑1 (MQ=255)

                     cagcagAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAat                                                                                                                                  <  1:167537/150‑1 (MQ=255)

                       gcagAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAatat                                                                                                                                <  1:3959027/150‑1 (MQ=255)

                         agagTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTc                                                                                                                              <  1:613908/150‑1 (MQ=255)

                           agTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCAt                                                                                                                            >  2:1144239/1‑150 (MQ=255)

                           agTGATTAAATCTGCATGGCTTTGATCGAGGGCCTCTTCCGGGGCGCTGATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCTTTGTTGACACACCTCTGGTCATGATAGTATCAATATTCAt                                                                                                                            <  2:2040742/150‑1 (MQ=255)

                             tGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGc                                                                                                                          <  2:3303961/150‑1 (MQ=255)

                               aTTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAg                                                                                                                        <  1:1226654/150‑1 (MQ=255)

                                ttAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGt                                                                                                                       <  2:2656541/150‑1 (MQ=255)

                                 tAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTa                                                                                                                      >  1:1527397/1‑150 (MQ=255)

                                 tAAATCTGCAGTGCTTTGATCGAGTGCCTCGTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAACTCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTa                                                                                                                      <  2:5099634/150‑1 (MQ=255)

                                  aaaTCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTAt                                                                                                                     <  2:3183992/150‑1 (MQ=255)

                                  aaaTCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTAt                                                                                                                     <  2:148448/150‑1 (MQ=255)

                                   aaTCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTAtt                                                                                                                    >  1:108583/1‑150 (MQ=255)

                                    aTCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTAttt                                                                                                                   <  2:3684441/150‑1 (MQ=255)

                                     tCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTa                                                                                                                  <  1:4628630/150‑1 (MQ=255)

                                     tCTGCATTGCTTTGATCGAGTGACTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTa                                                                                                                  >  1:4750772/1‑150 (MQ=255)

                                          aTTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAt                                                                                                             <  1:3243606/150‑1 (MQ=255)

                                           ttGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATa                                                                                                            <  2:495358/150‑1 (MQ=255)

                                             gCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaa                                                                                                          >  2:2794315/1‑150 (MQ=255)

                                             gCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaa                                                                                                          >  2:3224921/1‑150 (MQ=255)

                                              cTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaaa                                                                                                         >  1:249356/1‑150 (MQ=255)

                                               tttGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaaaa                                                                                                        >  1:802771/1‑150 (MQ=255)

                                               ttggATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAACTGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaaaa                                                                                                        <  2:4445467/147‑1 (MQ=255)

                                                ttGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAATATTTATGAATAAAAAt                                                                                                       <  2:4315023/150‑1 (MQ=255)

                                                ttGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAATATTTATGAATAAAAAt                                                                                                       <  2:1753429/150‑1 (MQ=255)

                                                   aTCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATaca                                                                                                    >  2:2521801/1‑150 (MQ=255)

                                                    tCGAGTGACTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATacac                                                                                                   >  1:1431392/1‑150 (MQ=255)

                                                      gAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTa                                                                                                 >  1:897661/1‑150 (MQ=255)

                                                       aGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTaa                                                                                                >  1:670423/1‑150 (MQ=255)

                                                         tGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACg                                                                                              <  2:1330690/150‑1 (MQ=255)

                                                           ccTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGtt                                                                                            <  2:4917110/150‑1 (MQ=255)

                                                             tctTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGa                                                                                          >  1:4100880/1‑150 (MQ=255)

                                                               ttCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGc                                                                                        >  2:2288222/1‑150 (MQ=255)

                                                                tCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCg                                                                                       >  2:1708992/1‑150 (MQ=255)

                                                                  cGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACAAACGTTGAGCGta                                                                                     >  2:1306061/1‑150 (MQ=255)

                                                                       cgcTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATaaa                                                                                <  2:3712513/150‑1 (MQ=255)

                                                                           tATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAAccc                                                                            >  1:1008957/1‑150 (MQ=255)

                                                                            aTTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACcca                                                                           >  2:4896734/1‑150 (MQ=255)

                                                                            aTTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACcca                                                                           >  1:3487731/1‑150 (MQ=255)

                                                                             ttGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACccac                                                                          >  1:3262112/1‑150 (MQ=255)

                                                                               gAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACccacca                                                                        <  2:15102/150‑1 (MQ=255)

                                                                                  ggTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGcc                                                                     >  1:533033/1‑150 (MQ=255)

                                                                                   gtgtGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCg                                                                    <  1:4230554/150‑1 (MQ=255)

                                                                                     gtgGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTa                                                                  >  1:2116795/1‑150 (MQ=255)

                                                                                       ggCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAg                                                                <  2:3643399/150‑1 (MQ=255)

                                                                                        gCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAgg                                                               <  1:785259/150‑1 (MQ=255)

                                                                                              agagCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATg                                                         <  1:3071182/150‑1 (MQ=255)

                                                                                                atcgcgGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGtt                                                       >  1:3402277/3‑150 (MQ=255)

                                                                                                agCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGtt                                                       <  2:3015403/150‑1 (MQ=255)

                                                                                                     ggTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACg                                                  >  1:2172013/1‑150 (MQ=255)

                                                                                                          aTCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTaa                                             >  1:3084732/1‑150 (MQ=255)

                                                                                                           tctcGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAAc                                            >  1:2913745/1‑150 (MQ=255)

                                                                                                            ctcGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAAcc                                           <  2:2731108/150‑1 (MQ=255)

                                                                                                            ctcGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAAcc                                           >  1:3313991/1‑150 (MQ=255)

                                                                                                               gATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTgg                                        >  2:4085380/1‑150 (MQ=255)

                                                                                                                aTAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGc                                       >  1:1972750/1‑150 (MQ=255)

                                                                                                                 tAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCa                                      >  1:933488/1‑150 (MQ=255)

                                                                                                                  aaaTGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGATGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCaa                                     >  1:1463582/1‑150 (MQ=255)

                                                                                                                   aaTGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAAc                                    >  1:1806894/1‑150 (MQ=255)

                                                                                                                         ggTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACa                              <  1:287577/150‑1 (MQ=255)

                                                                                                                             aTTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATaag                          >  2:1903338/1‑150 (MQ=255)

                                                                                                                                 gTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAgg                      >  1:2708712/1‑150 (MQ=255)

                                                                                                                                  tttttCATTGTTGACACACATCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGt                     <  1:4333328/150‑1 (MQ=255)

                                                                                                                                   ttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTg                    >  1:4454704/1‑150 (MQ=255)

                                                                                                                                    tttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTTAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGa                   <  1:1241493/150‑1 (MQ=255)

                                                                                                                                      tCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAAt                 >  1:4899927/1‑150 (MQ=255)

                                                                                                                                          tgttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGcc             >  2:1242984/1‑150 (MQ=255)

                                                                                                                                           gttgACACAACTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGTTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGccc            >  2:2623698/1‑150 (MQ=255)

                                                                                                                                            ttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGcccc           >  2:4987703/1‑150 (MQ=255)

                                                                                                                                              gACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGa         <  2:3904852/150‑1 (MQ=255)

                                                                                                                                               acacacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGAt        <  2:3368872/150‑1 (MQ=255)

                                                                                                                                               acacacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCAGAt        <  2:4750772/150‑1 (MQ=255)

                                                                                                                                                cacacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTATAACCTGGCAACCAGACATAAGAAGGTGAAAAGCCCCGATg       >  1:375194/1‑150 (MQ=255)

                                                                                                                                                 acacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGt      >  1:3812985/1‑150 (MQ=255)

                                                                                                                                                  cacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGtt     <  2:645050/150‑1 (MQ=255)

                                                                                                                                                   acCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTg    <  2:4213325/150‑1 (MQ=255)

                                                                                                                                                   acCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTg    >  1:2304290/1‑150 (MQ=255)

                                                                                                                                                     ctctGGTCATGATAGTATCAATATTCATGCAATATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGaa  <  2:3817675/150‑1 (MQ=255)

CAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCAACCAGACATAAGAAGGTGAATAGCCCCGATGTTGAA  >  CP009273/4144638‑4144936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A