Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,959,915 C→A 64.2% P178P (CCG→CCT)  flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,959,9150CA64.2% 163.2 / 310.9 246P178P (CCG→CCT) flhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (57/31);  new base A (95/63);  total (152/94)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 250 total aligned reads displayed.

TCACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959766‑1960064
                                                                                                                                                     |                                                                                                                                                     
tcaCGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATc                                                                                                                                                       <  2:463787/150‑1 (MQ=255)
tcaCGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAAGCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATc                                                                                                                                                       <  2:4337623/150‑1 (MQ=255)
  aCGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCgg                                                                                                                                                     >  2:606111/1‑150 (MQ=255)
   cGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGga                                                                                                                                                    <  1:3255190/150‑1 (MQ=255)
    gAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGgag                                                                                                                                                   >  1:1916479/1‑150 (MQ=255)
    gAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGgag                                                                                                                                                   >  1:60207/1‑150 (MQ=255)
      atatCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCCGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGAc                                                                                                                                                 >  2:3065879/1‑150 (MQ=255)
        atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTc                                                                                                                                               >  2:4116008/1‑150 (MQ=255)
         tCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCg                                                                                                                                              <  1:2051631/150‑1 (MQ=255)
         tCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCg                                                                                                                                              <  1:4300738/150‑1 (MQ=255)
          ccTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCgg                                                                                                                                             >  1:4521480/1‑150 (MQ=255)
          ccTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCgg                                                                                                                                             >  1:1956957/1‑150 (MQ=255)
          ccTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCgg                                                                                                                                             <  1:3773014/150‑1 (MQ=255)
             gCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCa                                                                                                                                          >  2:1352298/1‑150 (MQ=255)
              ccGTGACATACGCAGCTTTTTCAGGTGTCTGAATATTTTTAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATATCCCTACCAAATCCATCGCATTAACCATGGCGGTAATAGGAGACTCTTCCAt                                                                                                                                         <  2:2780497/150‑1 (MQ=255)
              ccGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCAt                                                                                                                                         >  1:3391094/1‑150 (MQ=255)
                gTGACCTACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCAGTACCCATGGCGGTAATAGGAGACTCGGCCATCa                                                                                                                                       <  2:3159391/150‑1 (MQ=255)
                 tGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCaa                                                                                                                                      >  1:546955/1‑150 (MQ=255)
                   aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAgc                                                                                                                                    >  2:4093058/1‑150 (MQ=255)
                   aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgc                                                                                                                                    >  2:3589526/1‑150 (MQ=255)
                   aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgc                                                                                                                                    >  1:1029906/1‑150 (MQ=255)
                     aTACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAgcgc                                                                                                                                  <  1:96670/150‑1 (MQ=255)
                     aTACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAgcgc                                                                                                                                  <  1:94298/150‑1 (MQ=255)
                        cGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGcatc                                                                                                                               >  2:2685031/1‑150 (MQ=255)
                            gCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCt                                                                                                                           <  1:1363947/150‑1 (MQ=255)
                             cTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTACTAGGAGACTCGGCCATCAAGCGCATCATCTg                                                                                                                          >  2:2346239/1‑150 (MQ=255)
                             cTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTg                                                                                                                          >  2:766907/1‑150 (MQ=255)
                             cTTTTTCACGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAATCACCAACAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCAAGGAGGTAATAGGAGACTCGGCCATCTAGCTCATCATCTg                                                                                                                          >  2:768139/1‑150 (MQ=255)
                              tttttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGc                                                                                                                         >  1:373926/1‑150 (MQ=255)
                              tttttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGc                                                                                                                         >  1:2233757/1‑150 (MQ=255)
                                tttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCgg                                                                                                                       >  1:816701/1‑150 (MQ=255)
                                  tCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGcc                                                                                                                     >  1:2487388/1‑150 (MQ=255)
                                   cAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCa                                                                                                                    >  2:3841825/1‑150 (MQ=255)
                                      gTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTg                                                                                                                 >  1:1056074/1‑150 (MQ=255)
                                        ggCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAt                                                                                                               <  2:3090952/150‑1 (MQ=255)
                                        ggCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAt                                                                                                               <  1:4031888/150‑1 (MQ=255)
                                           tGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGCTGcc                                                                                                            >  2:4354985/1‑150 (MQ=255)
                                           tGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGcc                                                                                                            >  2:2860620/1‑150 (MQ=255)
                                                atTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAgaga                                                                                                       <  1:4224567/150‑1 (MQ=255)
                                                 tttGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaa                                                                                                      >  1:1447619/1‑150 (MQ=255)
                                                   tGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                                    >  2:2025978/1‑150 (MQ=255)
                                                   tGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                                    >  1:1009859/1‑150 (MQ=255)
                                                       aaaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAcc                                                                                                >  1:4483074/1‑150 (MQ=255)
                                                        aaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                               >  2:3524155/1‑150 (MQ=255)
                                                        aaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                               >  2:4144405/1‑150 (MQ=255)
                                                        aaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                               >  2:972481/1‑150 (MQ=255)
                                                        aaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                               >  2:1282463/1‑150 (MQ=255)
                                                             cGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCa                                                                                          <  1:1266948/150‑1 (MQ=255)
                                                             cGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCa                                                                                          <  2:1919666/150‑1 (MQ=255)
                                                              gTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAc                                                                                         >  1:2765009/1‑150 (MQ=255)
                                                              gTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAc                                                                                         >  1:3167653/1‑150 (MQ=255)
                                                              gTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAc                                                                                         >  1:3166627/1‑150 (MQ=255)
                                                                cAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGc                                                                                       >  1:1891426/1‑150 (MQ=255)
                                                                cAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGc                                                                                       >  2:186826/1‑150 (MQ=255)
                                                                 aaaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCt                                                                                      >  1:592542/1‑150 (MQ=255)
                                                                        accaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAcc                                                                               >  1:1320980/1‑150 (MQ=255)
                                                                         ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCa                                                                              <  2:4222419/150‑1 (MQ=255)
                                                                          caTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAg                                                                             <  2:2277314/150‑1 (MQ=255)
                                                                           aTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAgg                                                                            >  1:2951963/1‑150 (MQ=255)
                                                                             tGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAt                                                                          >  2:3312326/1‑150 (MQ=255)
                                                                              ggAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATg                                                                         <  1:4139445/150‑1 (MQ=255)
                                                                               gAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATgg                                                                        >  2:3124988/1‑150 (MQ=255)
                                                                                aaTGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                                       >  2:4512863/1‑150 (MQ=255)
                                                                                    acacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTc                                                                   >  1:1709076/1‑150 (MQ=255)
                                                                                    acacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTc                                                                   <  2:4149582/150‑1 (MQ=255)
                                                                                        cAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCaaaa                                                               >  1:1584454/1‑150 (MQ=255)
                                                                                         aaGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAt                                                              <  2:3398598/150‑1 (MQ=255)
                                                                                          aGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAtt                                                             <  1:2407966/150‑1 (MQ=255)
                                                                                           gCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTg                                                            >  2:665107/1‑150 (MQ=255)
                                                                                              ccaccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCtt                                                         <  2:927814/150‑1 (MQ=255)
                                                                                               caccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCttt                                                        <  1:2854823/150‑1 (MQ=255)
                                                                                                accaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                                       >  2:2823583/1‑150 (MQ=255)
                                                                                                 ccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTaa                                                      <  1:3281019/150‑1 (MQ=255)
                                                                                                  cagcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAg                                                     <  2:1770125/150‑1 (MQ=255)
                                                                                                     cagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                                  >  2:101823/1‑150 (MQ=255)
                                                                                                     cagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                                  >  2:327043/1‑150 (MQ=255)
                                                                                                       gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACt                                                >  2:1461001/1‑150 (MQ=255)
                                                                                                         gcgcATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTcc                                              <  2:2599292/150‑1 (MQ=255)
                                                                                                              tAGCCCTACCAAATCCATCGCATTACCCATGGCGGTACTCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGcc                                         <  1:1099143/150‑1 (MQ=255)
                                                                                                                 cccTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGt                                      >  2:1524663/1‑150 (MQ=255)
                                                                                                                  ccTACCAAATCCATCTCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTc                                     <  1:4262906/150‑1 (MQ=255)
                                                                                                                   cTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTcg                                    >  1:3672275/1‑149 (MQ=255)
                                                                                                                   cTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCt                                    <  1:4010508/150‑1 (MQ=255)
                                                                                                                        aaaTCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                               >  1:3940097/1‑150 (MQ=255)
                                                                                                                        aaaTCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                               >  1:3940272/1‑150 (MQ=255)
                                                                                                                         aaTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCg                              <  1:2214495/150‑1 (MQ=255)
                                                                                                                          aTCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCga                             <  2:4264826/150‑1 (MQ=255)
                                                                                                                          aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCga                             <  1:3589526/150‑1 (MQ=255)
                                                                                                                          aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCga                             >  1:3509637/1‑150 (MQ=255)
                                                                                                                          aTCCATCGCATGACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCga                             <  1:433387/150‑1 (MQ=255)
                                                                                                                           tCCCTCGCATGACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCgag                            <  2:2934411/150‑1 (MQ=255)
                                                                                                                            ccATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCgaga                           <  1:3065879/150‑1 (MQ=255)
                                                                                                                              aTCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                         <  1:3364910/150‑1 (MQ=255)
                                                                                                                              aTCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                         <  1:67539/150‑1 (MQ=255)
                                                                                                                                   aTTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg                    >  2:2005521/1‑150 (MQ=255)
                                                                                                                                      aCCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGttt                 <  2:3253307/150‑1 (MQ=255)
                                                                                                                                              cGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCg         >  1:4199585/1‑150 (MQ=255)
                                                                                                                                                 tAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTa      >  1:3608901/1‑150 (MQ=255)
                                                                                                                                                  aaTAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAg     >  2:2124507/1‑150 (MQ=255)
                                                                                                                                                    tCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCg   <  2:3822089/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959766‑1960064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959768‑1960064
                                                                                                                                                   |                                                                                                                                                     
ACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGG                                                                                                                                                     >  SRR19973076.1268482/1‑150 (MQ=60)
ACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGG                                                                                                                                                     >  SRR19973076.1266911/1‑150 (MQ=60)
 CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGA                                                                                                                                                    >  SRR19973076.2603076/1‑150 (MQ=60)
 CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGA                                                                                                                                                    <  SRR19973076.3260000/150‑1 (MQ=60)
 CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGA                                                                                                                                                    <  SRR19973076.3259919/150‑1 (MQ=60)
  GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                   >  SRR19973076.1923056/1‑150 (MQ=60)
  GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                   >  SRR19973076.1919250/1‑150 (MQ=60)
  GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                   >  SRR19973076.1919485/1‑150 (MQ=60)
  GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                   >  SRR19973076.60345/1‑150 (MQ=60)
   AATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGA                                                                                                                                                  >  SRR19973076.213849/1‑150 (MQ=60)
       TCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCG                                                                                                                                              <  SRR19973076.2054624/150‑1 (MQ=60)
       TCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCG                                                                                                                                              <  SRR19973076.4307089/150‑1 (MQ=60)
        CCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGG                                                                                                                                             >  SRR19973076.1959799/1‑150 (MQ=60)
        CCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGG                                                                                                                                             >  SRR19973076.4528149/1‑150 (MQ=60)
        CCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGG                                                                                                                                             >  SRR19973076.4528187/1‑150 (MQ=60)
        CCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGG                                                                                                                                             <  SRR19973076.3778546/150‑1 (MQ=60)
          TGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCC                                                                                                                                           >  SRR19973076.3252407/1‑150 (MQ=60)
          TGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCC                                                                                                                                           >  SRR19973076.3252257/1‑150 (MQ=60)
            CCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCAT                                                                                                                                         >  SRR19973076.3396008/1‑150 (MQ=60)
             CGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATC                                                                                                                                        >  SRR19973076.2815483/1‑150 (MQ=60)
               TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                      >  SRR19973076.547816/1‑150 (MQ=60)
                 ACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGC                                                                                                                                    >  SRR19973076.1031437/1‑150 (MQ=60)
                   ATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGC                                                                                                                                  <  SRR19973076.94485/150‑1 (MQ=60)
                   ATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGC                                                                                                                                  <  SRR19973076.96861/150‑1 (MQ=60)
                   ATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGC                                                                                                                                  <  SRR19973076.995096/150‑1 (MQ=60)
                    TACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCA                                                                                                                                 >  SRR19973076.417581/1‑150 (MQ=60)
                       GCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCA                                                                                                                              <  SRR19973076.4465429/150‑1 (MQ=60)
                       GCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCA                                                                                                                              <  SRR19973076.930091/150‑1 (MQ=60)
                         AGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATC                                                                                                                            >  SRR19973076.945407/1‑150 (MQ=60)
                          GCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCT                                                                                                                           <  SRR19973076.1365955/150‑1 (MQ=60)
                            TTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGC                                                                                                                         >  SRR19973076.2236171/1‑150 (MQ=60)
                            TTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGC                                                                                                                         >  SRR19973076.2236968/1‑150 (MQ=60)
                            TTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGC                                                                                                                         <  SRR19973076.3645023/150‑1 (MQ=60)
                            TTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGC                                                                                                                         >  SRR19973076.374512/1‑150 (MQ=60)
                              TTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGG                                                                                                                       >  SRR19973076.817688/1‑150 (MQ=60)
                              TTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGG                                                                                                                       >  SRR19973076.817903/1‑150 (MQ=60)
                                TCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCC                                                                                                                     >  SRR19973076.2491004/1‑150 (MQ=60)
                                TCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCC                                                                                                                     >  SRR19973076.189674/1‑150 (MQ=60)
                                    GTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTG                                                                                                                 >  SRR19973076.1057640/1‑150 (MQ=60)
                                    GTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTG                                                                                                                 >  SRR19973076.1057636/1‑150 (MQ=60)
                                     TGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGA                                                                                                                <  SRR19973076.715641/150‑1 (MQ=60)
                                      GGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAT                                                                                                               <  SRR19973076.4037846/150‑1 (MQ=60)
                                        CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGC                                                                                                             <  SRR19973076.315214/150‑1 (MQ=60)
                                         TGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCC                                                                                                            >  SRR19973076.3959461/1‑150 (MQ=60)
                                           AATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAG                                                                                                          >  SRR19973076.4278338/1‑150 (MQ=60)
                                            ATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGA                                                                                                         <  SRR19973076.2059282/150‑1 (MQ=60)
                                              ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                       <  SRR19973076.4230737/150‑1 (MQ=60)
                                              ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                       <  SRR19973076.4230803/150‑1 (MQ=60)
                                              ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                       <  SRR19973076.4231602/150‑1 (MQ=60)
                                              ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                       >  SRR19973076.4299610/1‑150 (MQ=60)
                                               TTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCGAGCGCATCATCTGCGGCCAGTGATGCCAGAGAA                                                                                                      >  SRR19973076.1450046/1‑150 (MQ=60)
                                               TTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAA                                                                                                      <  SRR19973076.333043/150‑1 (MQ=60)
                                               TTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAA                                                                                                      >  SRR19973076.1449744/1‑150 (MQ=60)
                                                 TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                    >  SRR19973076.1011925/1‑150 (MQ=60)
                                                 TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                    >  SRR19973076.1011362/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.1347066/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                <  SRR19973076.1855875/150‑1 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.4488652/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.4489741/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.3899959/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.1347038/1‑150 (MQ=60)
                                                     AAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATATGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACC                                                                                                >  SRR19973076.3412883/1‑150 (MQ=60)
                                                           CGTCAACTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                          <  SRR19973076.3596821/150‑1 (MQ=60)
                                                           CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                          >  SRR19973076.3682686/1‑150 (MQ=60)
                                                           CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                          <  SRR19973076.1268887/150‑1 (MQ=60)
                                                           CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                          <  SRR19973076.1268826/150‑1 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.480860/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.2769025/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.480861/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         <  SRR19973076.3853396/150‑1 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         <  SRR19973076.1397458/150‑1 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.3171221/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.3172247/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.3172208/1‑150 (MQ=60)
                                                            GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                         >  SRR19973076.960367/1‑150 (MQ=60)
                                                              CAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGC                                                                                       >  SRR19973076.1894149/1‑150 (MQ=60)
                                                               AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                      >  SRR19973076.593458/1‑150 (MQ=60)
                                                               AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                      <  SRR19973076.2674737/150‑1 (MQ=60)
                                                               AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                      >  SRR19973076.593462/1‑150 (MQ=60)
                                                               AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                      >  SRR19973076.593483/1‑150 (MQ=60)
                                                                AATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTG                                                                                     >  SRR19973076.4308119/1‑150 (MQ=60)
                                                                    CCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAA                                                                                 <  SRR19973076.3335656/150‑1 (MQ=60)
                                                                     CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                                >  SRR19973076.2896144/1‑150 (MQ=60)
                                                                      ACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACC                                                                               >  SRR19973076.1322923/1‑150 (MQ=60)
                                                                         ATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGG                                                                            >  SRR19973076.2956258/1‑150 (MQ=60)
                                                                         ATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGG                                                                            >  SRR19973076.3984454/1‑150 (MQ=60)
                                                                         ATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCAACGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGG                                                                            >  SRR19973076.2957092/1‑150 (MQ=60)
                                                                            GGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATG                                                                         <  SRR19973076.4145564/150‑1 (MQ=60)
                                                                              AATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGT                                                                       <  SRR19973076.2410746/150‑1 (MQ=60)
                                                                                  ACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTC                                                                   >  SRR19973076.1711570/1‑150 (MQ=60)
                                                                                      CAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAA                                                               >  SRR19973076.1586788/1‑150 (MQ=60)
                                                                                      CAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAA                                                               >  SRR19973076.1586779/1‑150 (MQ=60)
                                                                                        AGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATT                                                             <  SRR19973076.104644/150‑1 (MQ=60)
                                                                                        AGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATT                                                             <  SRR19973076.2411455/150‑1 (MQ=60)
                                                                                        AGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATT                                                             <  SRR19973076.2411436/150‑1 (MQ=60)
                                                                                             CACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTT                                                        <  SRR19973076.2858957/150‑1 (MQ=60)
                                                                                               CCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAA                                                      <  SRR19973076.3285779/150‑1 (MQ=60)
                                                                                                 AGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGC                                                    <  SRR19973076.1534526/150‑1 (MQ=60)
                                                                                                  GCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCA                                                   <  SRR19973076.2270884/150‑1 (MQ=60)
                                                                                                    AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                                 <  SRR19973076.2866602/150‑1 (MQ=60)
                                                                                                    AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                                 <  SRR19973076.371241/150‑1 (MQ=60)
                                                                                                        CGCATAGCCCTACCAAATCCCTCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCG                                             <  SRR19973076.3141771/150‑1 (MQ=60)
                                                                                                            TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTACTCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                         <  SRR19973076.1100775/150‑1 (MQ=60)
                                                                                                            TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                         <  SRR19973076.1100684/150‑1 (MQ=60)
                                                                                                                CCTACCAAATCCATCTCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTC                                     <  SRR19973076.4269205/150‑1 (MQ=60)
                                                                                                                 CTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCT                                    <  SRR19973076.4016474/150‑1 (MQ=60)
                                                                                                                 CTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCT                                    >  SRR19973076.4046649/1‑150 (MQ=60)
                                                                                                                 CTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCT                                    <  SRR19973076.4016432/150‑1 (MQ=60)
                                                                                                                 CTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCG                                    >  SRR19973076.3677636/1‑150 (MQ=60)
                                                                                                                   ACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGA                                  <  SRR19973076.3421164/150‑1 (MQ=60)
                                                                                                                   ACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGA                                  <  SRR19973076.2028947/150‑1 (MQ=60)
                                                                                                                      AAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                               >  SRR19973076.3946085/1‑150 (MQ=60)
                                                                                                                      AAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                               >  SRR19973076.3945928/1‑150 (MQ=60)
                                                                                                                      AAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                               >  SRR19973076.3945910/1‑150 (MQ=60)
                                                                                                                       AATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCG                              <  SRR19973076.2217685/150‑1 (MQ=60)
                                                                                                                        ATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGA                             >  SRR19973076.3514726/1‑150 (MQ=60)
                                                                                                                        ATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGA                             >  SRR19973076.3514729/1‑150 (MQ=60)
                                                                                                                        ATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGA                             <  SRR19973076.3594741/150‑1 (MQ=60)
                                                                                                                        ATCCATCGCATGACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGA                             <  SRR19973076.434089/150‑1 (MQ=60)
                                                                                                                          CCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGA                           <  SRR19973076.3070330/150‑1 (MQ=60)
                                                                                                                           CATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAA                          >  SRR19973076.592818/1‑150 (MQ=60)
                                                                                                                            ATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                         <  SRR19973076.3369825/150‑1 (MQ=60)
                                                                                                                            ATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                         <  SRR19973076.3369791/150‑1 (MQ=60)
                                                                                                                            ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                         <  SRR19973076.67687/150‑1 (MQ=60)
                                                                                                                                       CATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAAT              <  SRR19973076.3777968/150‑1 (MQ=60)
                                                                                                                                            CGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCG         >  SRR19973076.4205786/1‑150 (MQ=60)
                                                                                                                                            CGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCG         >  SRR19973076.4205852/1‑150 (MQ=60)
                                                                                                                                              GTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGT       <  SRR19973076.4074204/150‑1 (MQ=60)
                                                                                                                                               TAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTA      >  SRR19973076.3614148/1‑150 (MQ=60)
                                                                                                                                               TAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTA      >  SRR19973076.3614044/1‑150 (MQ=60)
                                                                                                                                                AATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG     >  SRR19973076.186441/1‑150 (MQ=60)
                                                                                                                                                 ATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGC    <  SRR19973076.2272837/150‑1 (MQ=60)
                                                                                                                                                  TAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCG   <  SRR19973076.1743801/150‑1 (MQ=60)
                                                                                                                                                  TAGGAGACTCGGCAATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCATATTTGCTTTATGCTACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCG   <  SRR19973076.1743781/150‑1 (MQ=60)
                                                                                                                                                   CGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  SRR19973076.650660/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGCGG  >  CP009273/1959768‑1960064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑