Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,959,915 C→A 100% P178P (CCG→CCT)  flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,959,9150CA98.3% 600.2 / 1.0 190P178P (CCG→CCT) flhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (104/82);  total (106/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 190 total aligned reads displayed.

GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCC  >  CP009273/1959770‑1960055
                                                                                                                                                 |                                                                                                                                            
gAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAgg                                                                                                                                            <  1:3567010/148‑1 (MQ=255)
gAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAgg                                                                                                                                            <  1:1672341/148‑1 (MQ=255)
 aaTATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGga                                                                                                                                           >  1:3517502/1‑148 (MQ=255)
    atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGAc                                                                                                                                        >  1:477543/1‑148 (MQ=255)
    atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGAc                                                                                                                                        >  2:1582236/1‑148 (MQ=255)
    atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCAATGGCGGTAATCGGAGAc                                                                                                                                        >  2:3665042/1‑148 (MQ=255)
        tGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCgg                                                                                                                                    >  2:3028663/1‑148 (MQ=255)
            gTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCAt                                                                                                                                <  1:1504709/148‑1 (MQ=255)
            gTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCAt                                                                                                                                <  1:1292451/148‑1 (MQ=255)
              gACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCa                                                                                                                              <  2:3219705/148‑1 (MQ=255)
              gACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCa                                                                                                                              <  2:3219036/148‑1 (MQ=255)
               aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAGTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCaa                                                                                                                             >  1:785239/1‑148 (MQ=255)
                cATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAg                                                                                                                            >  2:693664/1‑148 (MQ=255)
                  tACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgcg                                                                                                                          >  2:3172061/1‑148 (MQ=255)
                        gCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGcatcat                                                                                                                    <  1:3106911/148‑1 (MQ=255)
                        gCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGcatcat                                                                                                                    >  1:1768462/1‑148 (MQ=255)
                         cTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCatcatc                                                                                                                   >  2:1931010/1‑148 (MQ=255)
                               cAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGc                                                                                                             <  1:1459134/148‑1 (MQ=255)
                                 ggTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCa                                                                                                           >  2:2716375/1‑148 (MQ=255)
                                 ggTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCa                                                                                                           >  1:3001591/1‑148 (MQ=255)
                                    ggCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTg                                                                                                        >  2:2641137/1‑148 (MQ=255)
                                      cTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAt                                                                                                      >  2:2589518/1‑148 (MQ=255)
                                        taaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGc                                                                                                    <  1:3769396/147‑1 (MQ=255)
                                        gAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGc                                                                                                    <  1:3065276/148‑1 (MQ=255)
                                        gAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGc                                                                                                    <  1:3066267/148‑1 (MQ=255)
                                        gAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAAGc                                                                                                    >  1:2826646/1‑148 (MQ=255)
                                         aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGcc                                                                                                   <  2:3732105/148‑1 (MQ=255)
                                         aaTATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGcc                                                                                                   >  1:2546417/1‑148 (MQ=255)
                                                 gAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                           >  1:3809600/1‑148 (MQ=255)
                                                 gAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                           >  1:1921832/1‑148 (MQ=255)
                                                 gAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaa                                                                                           >  1:686412/1‑148 (MQ=255)
                                                  aaaaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaaa                                                                                          <  2:852464/148‑1 (MQ=255)
                                                   aaaaGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaaaa                                                                                         >  1:1142819/1‑148 (MQ=255)
                                                      aGACGTCAAATCCCACCATTGGAATGACACCACGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                      <  1:1310294/148‑1 (MQ=255)
                                                      aGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                      >  2:2722711/1‑148 (MQ=255)
                                                      aGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAccc                                                                                      >  2:1803734/1‑148 (MQ=255)
                                                           tCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCa                                                                                 >  1:933443/1‑148 (MQ=255)
                                                             aaaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACg                                                                               <  1:1922645/148‑1 (MQ=255)
                                                             aaaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACg                                                                               <  2:1045224/148‑1 (MQ=255)
                                                              aaTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGc                                                                              >  2:332797/1‑148 (MQ=255)
                                                                 cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGc                                                                           <  1:854776/148‑1 (MQ=255)
                                                                 cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGc                                                                           >  2:3077726/1‑148 (MQ=255)
                                                                 cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGc                                                                           >  2:2999128/1‑148 (MQ=255)
                                                                 cccACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGc                                                                           >  2:452833/1‑148 (MQ=255)
                                                                   caccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCa                                                                         >  1:2557807/1‑148 (MQ=255)
                                                                     ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAc                                                                       >  1:1570839/1‑148 (MQ=255)
                                                                         tGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAgg                                                                   <  2:39385/148‑1 (MQ=255)
                                                                         tGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAgg                                                                   <  1:1833748/148‑1 (MQ=255)
                                                                         tGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAgg                                                                   <  1:2911776/148‑1 (MQ=255)
                                                                          ggAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGa                                                                  <  2:1506569/148‑1 (MQ=255)
                                                                          ggAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGa                                                                  <  1:2387011/148‑1 (MQ=255)
                                                                           gAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAt                                                                 >  2:791220/1‑148 (MQ=255)
                                                                             aTGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATgg                                                               <  1:2037164/148‑1 (MQ=255)
                                                                              tGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                              <  1:1433329/148‑1 (MQ=255)
                                                                              tGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                              >  2:3077136/1‑148 (MQ=255)
                                                                              tGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                              <  1:3015900/148‑1 (MQ=255)
                                                                              tGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGt                                                              <  1:3743168/148‑1 (MQ=255)
                                                                               gACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGtt                                                             <  2:1670143/148‑1 (MQ=255)
                                                                                acacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGttt                                                            >  2:55946/1‑148 (MQ=255)
                                                                                acacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGttt                                                            >  2:54873/1‑148 (MQ=255)
                                                                                acacCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGttt                                                            <  1:3256919/148‑1 (MQ=255)
                                                                                   ccAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCa                                                         >  2:1822948/1‑148 (MQ=255)
                                                                                       gCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAt                                                     <  2:1239362/148‑1 (MQ=255)
                                                                                           caccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCt                                                 <  2:299839/148‑1 (MQ=255)
                                                                                              cagcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                              >  2:854538/1‑148 (MQ=255)
                                                                                                   gTGCGCATAGCCCTACCACATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCACCCCGGATGGTTTTCAAAATTGCTTTAAGCaa                                         <  2:1360793/148‑1 (MQ=255)
                                                                                                   gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                         <  2:714304/148‑1 (MQ=255)
                                                                                                   gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATCGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                         <  2:221448/148‑1 (MQ=255)
                                                                                                             cccTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCa                               >  2:118438/1‑148 (MQ=255)
                                                                                                               cTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGt                             <  2:3292263/148‑1 (MQ=255)
                                                                                                                tACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTc                            >  2:2892595/1‑148 (MQ=255)
                                                                                                                tACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTc                            <  2:1174367/148‑1 (MQ=255)
                                                                                                                 aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCt                           <  2:2134900/148‑1 (MQ=255)
                                                                                                                  ccAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                          >  1:2481983/1‑148 (MQ=255)
                                                                                                                  ccAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                          >  1:1272553/1‑148 (MQ=255)
                                                                                                                  ccAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                          >  2:1061098/1‑148 (MQ=255)
                                                                                                                     aaTCCATCGCATTACCCCTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCCGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGc                       <  1:2967813/148‑1 (MQ=255)
                                                                                                                      aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                      >  1:1213607/1‑148 (MQ=255)
                                                                                                                      aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                      >  1:1213647/1‑148 (MQ=255)
                                                                                                                      aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                      >  1:2759561/1‑148 (MQ=255)
                                                                                                                      aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                      >  2:3165449/1‑148 (MQ=255)
                                                                                                                      aTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGcc                      >  1:73836/1‑148 (MQ=255)
                                                                                                                          aTCGCATTACCCCTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCGACTCCGCGCCAGTCTGAGCCgaga                  <  1:2183127/148‑1 (MQ=255)
                                                                                                                           tCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGaa                 >  2:2453736/1‑148 (MQ=255)
                                                                                                                           tCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGaa                 >  1:519506/1‑148 (MQ=255)
                                                                                                                           tCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGaa                 >  2:347101/1‑148 (MQ=255)
                                                                                                                            cGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                >  1:3535879/1‑148 (MQ=255)
                                                                                                                            cGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAc                >  1:2648434/1‑148 (MQ=255)
                                                                                                                              cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCCGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt              <  2:651572/148‑1 (MQ=255)
                                                                                                                              cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt              >  1:3373527/1‑148 (MQ=255)
                                                                                                                              cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt              >  2:154932/1‑148 (MQ=255)
                                                                                                                              cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt              >  2:2138802/1‑148 (MQ=255)
                                                                                                                              cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt              >  1:2659338/1‑148 (MQ=255)
                                                                                                                                 tACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGACCATCCg           >  2:2013112/1‑148 (MQ=255)
                                                                                                                                 tACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg           >  1:982812/1‑148 (MQ=255)
                                                                                                                                 tACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg           >  1:2469492/1‑148 (MQ=255)
                                                                                                                                    ccATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGttt        <  2:75801/148‑1 (MQ=255)
                                                                                                                                      aTGGCGTTAATAGGAGACTCGCCCAACAAGCGCATCATCTGCGGCCAGTGATGCCAGACAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTaa      <  1:3197530/148‑1 (MQ=255)
                                                                                                                                      aTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTaa      <  2:95112/148‑1 (MQ=255)
                                                                                                                                        ggCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATg    <  2:595464/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCC  >  CP009273/1959770‑1960055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGC  >  CP009273/1959766‑1960062
                                                                                                                                                     |                                                                                                                                                   
TCACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATA                                                                                                                                                     <  SRR19973069.1541056/150‑1 (MQ=60)
   CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGA                                                                                                                                                  >  SRR19973069.3540199/1‑150 (MQ=60)
    GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                 <  SRR19973069.3590236/150‑1 (MQ=60)
    GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                 <  SRR19973069.1683970/150‑1 (MQ=60)
      ATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGAC                                                                                                                                               >  SRR19973069.481008/1‑150 (MQ=60)
                GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                     <  SRR19973069.3217453/150‑1 (MQ=60)
                GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                     <  SRR19973069.1301085/150‑1 (MQ=60)
                GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                     <  SRR19973069.1301115/150‑1 (MQ=60)
                GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                     <  SRR19973069.1515134/150‑1 (MQ=60)
                 TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAGTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                    >  SRR19973069.790807/1‑150 (MQ=60)
                  GACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAG                                                                                                                                   <  SRR19973069.3223098/150‑1 (MQ=60)
                          CAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCAT                                                                                                                           >  SRR19973069.1781112/1‑150 (MQ=60)
                          CAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCAT                                                                                                                           >  SRR19973069.1780925/1‑150 (MQ=60)
                          CAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCAT                                                                                                                           >  SRR19973069.786999/1‑150 (MQ=60)
                           AGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATC                                                                                                                          >  SRR19973069.1935472/1‑150 (MQ=60)
                            GCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCT                                                                                                                         <  SRR19973069.3127350/150‑1 (MQ=60)
                               TTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCG                                                                                                                      <  SRR19973069.3841374/150‑1 (MQ=60)
                                   CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                  <  SRR19973069.1469313/150‑1 (MQ=60)
                                   CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                  >  SRR19973069.3021510/1‑150 (MQ=60)
                                   CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                  >  SRR19973069.3020119/1‑150 (MQ=60)
                                    AGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAG                                                                                                                 <  SRR19973069.3496625/150‑1 (MQ=60)
                                          CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGC                                                                                                           >  SRR19973069.2845341/1‑150 (MQ=60)
                                          CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAAGC                                                                                                           >  SRR19973069.2845334/1‑150 (MQ=60)
                                           TGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCC                                                                                                          >  SRR19973069.2563251/1‑150 (MQ=60)
                                            TAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCA                                                                                                         <  SRR19973069.3793885/150‑1 (MQ=60)
                                            GAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCA                                                                                                         <  SRR19973069.3085543/150‑1 (MQ=60)
                                            GAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCA                                                                                                         <  SRR19973069.3086536/150‑1 (MQ=60)
                                              ATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGA                                                                                                       >  SRR19973069.726066/1‑150 (MQ=60)
                                                   TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                  >  SRR19973069.1935049/1‑150 (MQ=60)
                                                   TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                  >  SRR19973069.3834260/1‑150 (MQ=60)
                                                   TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                  >  SRR19973069.691203/1‑150 (MQ=60)
                                                     GAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAA                                                                                                >  SRR19973069.1150798/1‑150 (MQ=60)
                                                        AAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCC                                                                                             >  SRR19973069.2979284/1‑150 (MQ=60)
                                                          AGACGTCAAATCCCACCATTGGAATGACACCACGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCG                                                                                           <  SRR19973069.1319252/150‑1 (MQ=60)
                                                             CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                        >  SRR19973069.939772/1‑150 (MQ=60)
                                                             CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                        >  SRR19973069.1268654/1‑150 (MQ=60)
                                                                 AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                    <  SRR19973069.1935863/150‑1 (MQ=60)
                                                                  AATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTG                                                                                   >  SRR19973069.2418462/1‑150 (MQ=60)
                                                                  AATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTG                                                                                   >  SRR19973069.96082/1‑150 (MQ=60)
                                                                   ATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGC                                                                                  >  SRR19973069.901767/1‑150 (MQ=60)
                                                                    TCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATAATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCC                                                                                 <  SRR19973069.1491166/150‑1 (MQ=60)
                                                                     CCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATTAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCA                                                                                <  SRR19973069.2399745/150‑1 (MQ=60)
                                                                     CCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCA                                                                                <  SRR19973069.860767/150‑1 (MQ=60)
                                                                     CCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCA                                                                                >  SRR19973069.2574696/1‑150 (MQ=60)
                                                                     CCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCA                                                                                <  SRR19973069.1365363/150‑1 (MQ=60)
                                                                      CCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAA                                                                               >  SRR19973069.2581520/1‑150 (MQ=60)
                                                                       CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                              >  SRR19973069.1581529/1‑150 (MQ=60)
                                                                       CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                              >  SRR19973069.822046/1‑150 (MQ=60)
                                                                             TGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAT                                                                        <  SRR19973069.3119906/150‑1 (MQ=60)
                                                                             TGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAT                                                                        <  SRR19973069.2931037/150‑1 (MQ=60)
                                                                             TGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAT                                                                        <  SRR19973069.3747255/150‑1 (MQ=60)
                                                                             TGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAT                                                                        <  SRR19973069.1846590/150‑1 (MQ=60)
                                                                              GGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATG                                                                       <  SRR19973069.2403191/150‑1 (MQ=60)
                                                                                 ATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTT                                                                    <  SRR19973069.2051315/150‑1 (MQ=60)
                                                                                  TGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTT                                                                   <  SRR19973069.3767559/150‑1 (MQ=60)
                                                                                  TGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTT                                                                   <  SRR19973069.3767673/150‑1 (MQ=60)
                                                                                  TGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTT                                                                   <  SRR19973069.3035908/150‑1 (MQ=60)
                                                                                  TGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTT                                                                   <  SRR19973069.1443331/150‑1 (MQ=60)
                                                                                   GACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCTCATTACGCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAACAGGATGGTTTT                                                                  <  SRR19973069.2174979/150‑1 (MQ=60)
                                                                                   GACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTT                                                                  <  SRR19973069.1318767/150‑1 (MQ=60)
                                                                                    ACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTC                                                                 <  SRR19973069.3278252/150‑1 (MQ=60)
                                                                                               CACCAGCAGTGCGCATAGCCATACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTT                                                      <  SRR19973069.1592968/150‑1 (MQ=60)
                                                                                                    GCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCA                                                 <  SRR19973069.1531028/150‑1 (MQ=60)
                                                                                                      AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                               >  SRR19973069.3045341/1‑150 (MQ=60)
                                                                                                       GTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACT                                              <  SRR19973069.10023/150‑1 (MQ=60)
                                                                                                       GTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACT                                              <  SRR19973069.1958593/150‑1 (MQ=60)
                                                                                                                    TACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTG                                 >  SRR19973069.1281103/1‑150 (MQ=60)
                                                                                                                    TACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTG                                 >  SRR19973069.2498520/1‑150 (MQ=60)
                                                                                                                    TACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTG                                 >  SRR19973069.2498601/1‑150 (MQ=60)
                                                                                                                     ACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGA                                >  SRR19973069.1447508/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.2778861/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.1221930/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.74663/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.1221934/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.1221970/1‑150 (MQ=60)
                                                                                                                        AAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCC                             >  SRR19973069.2777979/1‑150 (MQ=60)
                                                                                                                         AATCCATCGCATTACCCCTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCCGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCG                            <  SRR19973069.2987510/150‑1 (MQ=60)
                                                                                                                         AATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCG                            <  SRR19973069.1898618/150‑1 (MQ=60)
                                                                                                                             CATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAA                        >  SRR19973069.493203/1‑150 (MQ=60)
                                                                                                                             CATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAA                        >  SRR19973069.523196/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCCTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCGACTCCGCGCCAGTCTGAGCCGAGAAC                       <  SRR19973069.2198001/150‑1 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       >  SRR19973069.3558804/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       >  SRR19973069.3559096/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       >  SRR19973069.3558264/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       >  SRR19973069.2666174/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       >  SRR19973069.2666188/1‑150 (MQ=60)
                                                                                                                              ATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAAC                       <  SRR19973069.2760956/150‑1 (MQ=60)
                                                                                                                                CGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAT                     >  SRR19973069.3395585/1‑150 (MQ=60)
                                                                                                                                CGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAT                     >  SRR19973069.2677163/1‑150 (MQ=60)
                                                                                                                                 GCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATC                    >  SRR19973069.2529953/1‑150 (MQ=60)
                                                                                                                                   ATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCG                  >  SRR19973069.989453/1‑150 (MQ=60)
                                                                                                                                   ATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCG                  >  SRR19973069.2486063/1‑150 (MQ=60)
                                                                                                                                      ACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGACCATCCGTTT               >  SRR19973069.3393169/1‑150 (MQ=60)
                                                                                                                                      ACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTT               <  SRR19973069.589312/150‑1 (MQ=60)
                                                                                                                                          ATGGCGTTAATAGGAGACTCGCCCAACAAGCGCATCATCTGCGGCCAGTGATGCCAGACAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATG           <  SRR19973069.3218311/150‑1 (MQ=60)
                                                                                                                                          ATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATG           >  SRR19973069.3022415/1‑150 (MQ=60)
                                                                                                                                                  AATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG   >  SRR19973069.2687360/1‑150 (MQ=60)
                                                                                                                                                  AATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG   >  SRR19973069.1099037/1‑150 (MQ=60)
                                                                                                                                                  AATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG   >  SRR19973069.1098987/1‑150 (MQ=60)
                                                                                                                                                   ATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGC  >  SRR19973069.526983/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TCACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAGC  >  CP009273/1959766‑1960062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑