Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,959,915 C→A 100% P178P (CCG→CCT)  flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,959,9150CA99.2% 833.7 / ‑1.6 252P178P (CCG→CCT) flhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (149/99);  total (152/100)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGT  >  CP009273/1959769‑1960059
                                                                                                                                                  |                                                                                                                                                
cGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAgg                                                                                                                                                <  2:1903225/149‑1 (MQ=255)
  aaTATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGgag                                                                                                                                              >  1:2451033/1‑149 (MQ=255)
  aaTATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATAGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGgag                                                                                                                                              >  1:2312823/1‑149 (MQ=255)
    tatCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGAc                                                                                                                                            <  2:729782/149‑1 (MQ=255)
     atCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTACTAGGAGACt                                                                                                                                           >  2:1263782/1‑149 (MQ=255)
       ccTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCg                                                                                                                                         >  2:2785777/1‑149 (MQ=255)
        cTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCgg                                                                                                                                        >  2:1405686/1‑149 (MQ=255)
         tGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGc                                                                                                                                       >  2:902841/1‑149 (MQ=255)
          gCCGTGACATACGCAGCTTTTTCAGTTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCAATCGCATTACCCATGGCGGTAATAGGAGACTCGGcc                                                                                                                                      >  1:3653449/1‑149 (MQ=255)
             gTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATc                                                                                                                                   <  1:1502812/149‑1 (MQ=255)
               gACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCATATCCCACCATTGGAATGACTCCAAGCACCACCAGCTGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCaa                                                                                                                                 <  1:3962699/149‑1 (MQ=255)
                aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAg                                                                                                                                >  1:1152067/1‑149 (MQ=255)
                aCATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAg                                                                                                                                <  1:3440115/149‑1 (MQ=255)
                 cATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgc                                                                                                                               <  2:4635494/149‑1 (MQ=255)
                  aTACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgcg                                                                                                                              >  1:513678/1‑149 (MQ=255)
                   tACGCAGCTTTTTCAGGTGGCTGAATCTTTGGAAAAAGTCGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCTCATAGCCCTACCAAATCCATCGCTTTACACATGGCGGTAATAGGAGACTCGGCCATCAAgcgc                                                                                                                             <  1:2127056/149‑1 (MQ=255)
                   tACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAgcgc                                                                                                                             <  1:3299776/149‑1 (MQ=255)
                      gCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGcatc                                                                                                                          <  2:1997521/149‑1 (MQ=255)
                        aGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGcatcat                                                                                                                        >  2:3556363/1‑149 (MQ=255)
                           tttttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTg                                                                                                                     >  2:2685195/1‑149 (MQ=255)
                              ttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCgg                                                                                                                  <  2:4445182/149‑1 (MQ=255)
                              ttCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCgg                                                                                                                  <  2:4251913/149‑1 (MQ=255)
                                cAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGcc                                                                                                                <  1:1663739/149‑1 (MQ=255)
                                 aGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCa                                                                                                               >  1:2733209/1‑149 (MQ=255)
                                  ggTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAg                                                                                                              >  2:4694740/1‑149 (MQ=255)
                                   gTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGt                                                                                                             <  1:1000740/149‑1 (MQ=255)
                                   gTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGt                                                                                                             >  2:3207601/1‑149 (MQ=255)
                                     ggCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGa                                                                                                           <  1:2051298/149‑1 (MQ=255)
                                      gCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAt                                                                                                          >  2:1772032/1‑149 (MQ=255)
                                       cTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATg                                                                                                         >  2:2902182/1‑149 (MQ=255)
                                        tGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGc                                                                                                        >  1:1381834/1‑149 (MQ=255)
                                           atatTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCag                                                                                                     >  1:169730/1‑149 (MQ=255)
                                            tatTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCaga                                                                                                    >  2:2177095/1‑149 (MQ=255)
                                            tatTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCaga                                                                                                    >  2:2875020/1‑149 (MQ=255)
                                              tttGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAgaga                                                                                                  >  1:3850011/1‑149 (MQ=255)
                                                  gAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATAGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGaaaaa                                                                                              >  2:3648487/1‑149 (MQ=255)
                                                       aGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAcccc                                                                                         <  2:2770263/149‑1 (MQ=255)
                                                        gACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCg                                                                                        <  1:249510/149‑1 (MQ=255)
                                                           gTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCa                                                                                     >  1:1588701/1‑149 (MQ=255)
                                                            tCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAc                                                                                    >  1:3837900/1‑149 (MQ=255)
                                                             cAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACg                                                                                   >  2:3958663/1‑149 (MQ=255)
                                                             cAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACg                                                                                   >  2:3259494/1‑149 (MQ=255)
                                                               aaTCACACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCt                                                                                 <  1:3904398/149‑1 (MQ=255)
                                                                aTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTg                                                                                >  2:507571/1‑149 (MQ=255)
                                                                aTCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTg                                                                                >  2:381754/1‑149 (MQ=255)
                                                                  cccACCATGGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGcc                                                                              <  2:2561475/149‑1 (MQ=255)
                                                                   ccaccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCa                                                                             >  2:2126489/1‑149 (MQ=255)
                                                                    caccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCaa                                                                            >  2:4568335/1‑149 (MQ=255)
                                                                     accaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAc                                                                           >  1:3155867/1‑149 (MQ=255)
                                                                     accaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAc                                                                           >  1:3650637/1‑149 (MQ=255)
                                                                      ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAcc                                                                          <  1:774395/149‑1 (MQ=255)
                                                                      ccaTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAcc                                                                          >  2:3834219/1‑149 (MQ=255)
                                                                        aTTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAg                                                                        >  1:3550355/1‑149 (MQ=255)
                                                                           ggAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGAt                                                                     <  1:431694/149‑1 (MQ=255)
                                                                            gAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATg                                                                    <  2:956211/149‑1 (MQ=255)
                                                                                gACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGttt                                                                <  2:171227/149‑1 (MQ=255)
                                                                                   acCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCa                                                             >  2:1496472/1‑149 (MQ=255)
                                                                                   acCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCa                                                             >  2:3847030/1‑149 (MQ=255)
                                                                                      aaGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCaaaa                                                          >  1:4661426/1‑149 (MQ=255)
                                                                                       aGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTACTAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAt                                                         >  2:4341717/1‑149 (MQ=255)
                                                                                       aGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAt                                                         <  1:3091565/149‑1 (MQ=255)
                                                                                        gCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAtt                                                        >  2:105967/1‑149 (MQ=255)
                                                                                        gCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAAtt                                                        >  2:2293202/1‑149 (MQ=255)
                                                                                           ccaccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCt                                                     >  2:1386737/1‑149 (MQ=255)
                                                                                            caccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCtt                                                    <  1:4229104/149‑1 (MQ=255)
                                                                                             accaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCttt                                                   <  2:2486555/149‑1 (MQ=255)
                                                                                              ccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                                  >  2:1493912/1‑149 (MQ=255)
                                                                                              ccaGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTa                                                  <  1:2635677/149‑1 (MQ=255)
                                                                                               cagcagTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCCGTGATGCCCGAGAAAAAACCCCGTCACGCTGCCACCCAGGATGGTTTTCAAAATTGCTTTaa                                                 >  2:1920476/1‑149 (MQ=255)
                                                                                                   agTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                             <  1:2062263/149‑1 (MQ=255)
                                                                                                   agTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCaa                                             <  1:6319/149‑1 (MQ=255)
                                                                                                    gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAc                                            >  1:2211359/1‑149 (MQ=255)
                                                                                                    gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAc                                            <  2:4542084/149‑1 (MQ=255)
                                                                                                    gTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAc                                            <  1:4726776/149‑1 (MQ=255)
                                                                                                      gcgcATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTc                                          <  1:4689760/149‑1 (MQ=255)
                                                                                                        gcATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCcg                                        >  2:4160908/1‑149 (MQ=255)
                                                                                                            aGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGcc                                    >  1:551216/1‑149 (MQ=255)
                                                                                                              cccTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAg                                  <  1:647069/149‑1 (MQ=255)
                                                                                                              cccTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAg                                  <  1:3038495/149‑1 (MQ=255)
                                                                                                                 tACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCt                               <  2:2431663/149‑1 (MQ=255)
                                                                                                                  aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                              <  2:4367774/149‑1 (MQ=255)
                                                                                                                  aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                              >  1:901987/1‑149 (MQ=255)
                                                                                                                  aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                              >  2:3669759/1‑149 (MQ=255)
                                                                                                                  aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                              >  1:1294243/1‑149 (MQ=255)
                                                                                                                  aCCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTg                              >  2:1279892/1‑149 (MQ=255)
                                                                                                                     aaaTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGc                           >  1:1041476/1‑149 (MQ=255)
                                                                                                                     aaaTCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGc                           <  2:3146715/149‑1 (MQ=255)
                                                                                                                              gCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt                  >  2:2499561/1‑149 (MQ=255)
                                                                                                                              gCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACAt                  >  2:1166779/1‑149 (MQ=255)
                                                                                                                               cATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATc                 >  1:1956624/1‑149 (MQ=255)
                                                                                                                                 ttACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCg               <  1:955471/149‑1 (MQ=255)
                                                                                                                                  tACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGt              <  1:4750723/149‑1 (MQ=255)
                                                                                                                                  tACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGt              <  1:1480580/149‑1 (MQ=255)
                                                                                                                                   aCCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGtt             <  1:3015619/149‑1 (MQ=255)
                                                                                                                                   aCCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGtt             >  1:1072680/1‑149 (MQ=255)
                                                                                                                                      cATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTaa          >  2:3531016/1‑149 (MQ=255)
                                                                                                                                       aTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAAt         >  1:2698638/1‑149 (MQ=255)
                                                                                                                                       aTGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAAt         >  2:861573/1‑149 (MQ=255)
                                                                                                                                         ggCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGc       >  2:1811681/1‑149 (MQ=255)
                                                                                                                                            ggTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCg    >  2:4181579/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGT  >  CP009273/1959769‑1960059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG  >  CP009273/1959767‑1960061
                                                                                                                                                    |                                                                                                                                                  
CACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAG                                                                                                                                                   <  SRR19973071.2436027/150‑1 (MQ=60)
   GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                >  SRR19973071.2459938/1‑150 (MQ=60)
   GAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATAGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAG                                                                                                                                                >  SRR19973071.2321260/1‑150 (MQ=60)
      TATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACT                                                                                                                                             <  SRR19973071.906100/150‑1 (MQ=60)
         CCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGG                                                                                                                                          <  SRR19973071.3894633/150‑1 (MQ=60)
           TGCCGTGACATACGCAGCTTTTTCAGTTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCAATCGCATTACCCATGGCGGTAATAGGAGACTCGGCC                                                                                                                                        >  SRR19973071.3666304/1‑150 (MQ=60)
           TGCCGTGACATACGCAGC‑TTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCA                                                                                                                                       <  SRR19973071.1302636/150‑1 (MQ=60)
               GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                    <  SRR19973071.1508253/150‑1 (MQ=60)
               GTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCA                                                                                                                                    <  SRR19973071.1079459/150‑1 (MQ=60)
                TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                   >  SRR19973071.767282/1‑150 (MQ=60)
                TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                   >  SRR19973071.3165716/1‑150 (MQ=60)
                TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                   >  SRR19973071.3045591/1‑150 (MQ=60)
                TGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAA                                                                                                                                   >  SRR19973071.3165724/1‑150 (MQ=60)
                 GACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCATATCCCACCATTGGAATGACTCCAAGCACCACCAGCTGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAG                                                                                                                                  <  SRR19973071.3976601/150‑1 (MQ=60)
                 GACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAG                                                                                                                                  >  SRR19973071.1156075/1‑150 (MQ=60)
                  ACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGC                                                                                                                                 <  SRR19973071.3452261/150‑1 (MQ=60)
                   CATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCG                                                                                                                                >  SRR19973071.515597/1‑150 (MQ=60)
                    ATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGC                                                                                                                               <  SRR19973071.4290728/150‑1 (MQ=60)
                    ATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGC                                                                                                                               >  SRR19973071.2120451/1‑150 (MQ=60)
                     TACGCAGCTTTTTCAGGTGGCTGAATCTTTGGAAAAAGTCGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCTCATAGCCCTACCAAATCCATCGCTTTACACATGGCGGTAATAGGAGACTCGGCCATCAAGCGCA                                                                                                                              <  SRR19973071.2134914/150‑1 (MQ=60)
                     TACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCA                                                                                                                              <  SRR19973071.3311472/150‑1 (MQ=60)
                           GCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCT                                                                                                                        >  SRR19973071.2697996/1‑150 (MQ=60)
                            CTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTG                                                                                                                       >  SRR19973071.1516605/1‑150 (MQ=60)
                                  CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                 >  SRR19973071.2742916/1‑150 (MQ=60)
                                  CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                 <  SRR19973071.1669833/150‑1 (MQ=60)
                                  CAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCA                                                                                                                 >  SRR19973071.402315/1‑150 (MQ=60)
                                   AGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAG                                                                                                                >  SRR19973071.4567950/1‑150 (MQ=60)
                                     GTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTG                                                                                                              >  SRR19973071.486761/1‑150 (MQ=60)
                                     GTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTG                                                                                                              <  SRR19973071.1004377/150‑1 (MQ=60)
                                      TGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGA                                                                                                             >  SRR19973071.972282/1‑150 (MQ=60)
                                       GGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAT                                                                                                            <  SRR19973071.2058827/150‑1 (MQ=60)
                                       GGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAT                                                                                                            <  SRR19973071.2482014/150‑1 (MQ=60)
                                         CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGC                                                                                                          >  SRR19973071.1069914/1‑150 (MQ=60)
                                         CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGC                                                                                                          >  SRR19973071.1386926/1‑150 (MQ=60)
                                         CTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGC                                                                                                          >  SRR19973071.2495602/1‑150 (MQ=60)
                                          TGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACGCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCC                                                                                                         <  SRR19973071.564048/150‑1 (MQ=60)
                                            AATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAG                                                                                                       >  SRR19973071.170456/1‑150 (MQ=60)
                                               ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                    >  SRR19973071.3863650/1‑150 (MQ=60)
                                               ATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGA                                                                                                    >  SRR19973071.3863635/1‑150 (MQ=60)
                                                  TGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAA                                                                                                 >  SRR19973071.2526236/1‑150 (MQ=60)
                                                   GGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAA                                                                                                <  SRR19973071.3407304/150‑1 (MQ=60)
                                                   GGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAA                                                                                                <  SRR19973071.3407307/150‑1 (MQ=60)
                                                    GAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAA                                                                                               >  SRR19973071.48230/1‑150 (MQ=60)
                                                    GAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAA                                                                                               >  SRR19973071.2931803/1‑150 (MQ=60)
                                                        AAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCC                                                                                           <  SRR19973071.1046816/150‑1 (MQ=60)
                                                        AAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCC                                                                                           <  SRR19973071.1409054/150‑1 (MQ=60)
                                                         AGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCG                                                                                          >  SRR19973071.3672361/1‑150 (MQ=60)
                                                          GACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGT                                                                                         <  SRR19973071.250455/150‑1 (MQ=60)
                                                            CGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCA                                                                                       >  SRR19973071.1594316/1‑150 (MQ=60)
                                                             GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                      >  SRR19973071.3851502/1‑150 (MQ=60)
                                                             GTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCAC                                                                                      >  SRR19973071.1788930/1‑150 (MQ=60)
                                                              TCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACG                                                                                     <  SRR19973071.2376856/150‑1 (MQ=60)
                                                                AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                   <  SRR19973071.869479/150‑1 (MQ=60)
                                                                AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                   >  SRR19973071.2057540/1‑150 (MQ=60)
                                                                AAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCT                                                                                   >  SRR19973071.2057547/1‑150 (MQ=60)
                                                                 AATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTG                                                                                  <  SRR19973071.1497482/150‑1 (MQ=60)
                                                                 AATCACACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTG                                                                                  <  SRR19973071.3918177/150‑1 (MQ=60)
                                                                  ATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGC                                                                                 >  SRR19973071.4514065/1‑150 (MQ=60)
                                                                      CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                             >  SRR19973071.3166903/1‑150 (MQ=60)
                                                                      CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                             >  SRR19973071.3663482/1‑150 (MQ=60)
                                                                      CACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAAC                                                                             >  SRR19973071.3663680/1‑150 (MQ=60)
                                                                        CCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCA                                                                           <  SRR19973071.777004/150‑1 (MQ=60)
                                                                         CATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAG                                                                          >  SRR19973071.3562744/1‑150 (MQ=60)
                                                                         CATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAG                                                                          >  SRR19973071.2287487/1‑150 (MQ=60)
                                                                           TTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGA                                                                        <  SRR19973071.2701956/150‑1 (MQ=60)
                                                                             GGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATG                                                                      <  SRR19973071.1173323/150‑1 (MQ=60)
                                                                             GGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATG                                                                      <  SRR19973071.4690754/150‑1 (MQ=60)
                                                                             GGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATG                                                                      <  SRR19973071.433189/150‑1 (MQ=60)
                                                                                ATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTT                                                                   <  SRR19973071.1606206/150‑1 (MQ=60)
                                                                                       CAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAA                                                            >  SRR19973071.4678026/1‑150 (MQ=60)
                                                                                         AGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATT                                                          <  SRR19973071.3102485/150‑1 (MQ=60)
                                                                                         AGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATT                                                          <  SRR19973071.3102402/150‑1 (MQ=60)
                                                                                              CACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTT                                                     <  SRR19973071.4244139/150‑1 (MQ=60)
                                                                                               ACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTA                                                    <  SRR19973071.1408715/150‑1 (MQ=60)
                                                                                                CCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAA                                                   <  SRR19973071.2645169/150‑1 (MQ=60)
                                                                                                    CAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAA                                               <  SRR19973071.1676453/150‑1 (MQ=60)
                                                                                                    CAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAA                                               <  SRR19973071.1676512/150‑1 (MQ=60)
                                                                                                     AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                              >  SRR19973071.2219535/1‑150 (MQ=60)
                                                                                                     AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                              <  SRR19973071.2069793/150‑1 (MQ=60)
                                                                                                     AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                              <  SRR19973071.2069846/150‑1 (MQ=60)
                                                                                                     AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAAC                                              <  SRR19973071.6371/150‑1 (MQ=60)
                                                                                                     AGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAAACCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATAGCTTTAAGCAAC                                              >  SRR19973071.3222638/1‑150 (MQ=60)
                                                                                                      GTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACT                                             <  SRR19973071.4743521/150‑1 (MQ=60)
                                                                                                       TGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTC                                            <  SRR19973071.1560772/150‑1 (MQ=60)
                                                                                                        GCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCC                                           <  SRR19973071.4706422/150‑1 (MQ=60)
                                                                                                        GCGCATAGCCCTACCAAAGCCATCGCATTACCCATGGCGTTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGAGGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGTTGGTTTTCAAAATTGCTTTAAGCAACTCC                                           <  SRR19973071.4706600/150‑1 (MQ=60)
                                                                                                             TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                      >  SRR19973071.3528493/1‑150 (MQ=60)
                                                                                                             TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                      >  SRR19973071.553287/1‑150 (MQ=60)
                                                                                                             TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                      >  SRR19973071.553288/1‑150 (MQ=60)
                                                                                                             TAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                      >  SRR19973071.3102183/1‑150 (MQ=60)
                                                                                                             TAGCCCTACCAAATCCATCGCATTACCCAGGGCGGTAATTGGAGACTCGGCAATAAAGCGCATCATCTGGGGCCAGTGATGCCTGATAAAAAACCCCGTAACGCTGCCAACCTGGTTGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCC                                      <  SRR19973071.2916626/150‑1 (MQ=60)
                                                                                                               GCCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAG                                    >  SRR19973071.2146900/1‑150 (MQ=60)
                                                                                                                CCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGT                                   <  SRR19973071.3049302/150‑1 (MQ=60)
                                                                                                                CCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGT                                   <  SRR19973071.649077/150‑1 (MQ=60)
                                                                                                                CCCTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGT                                   <  SRR19973071.649440/150‑1 (MQ=60)
                                                                                                                  CTACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCT                                 <  SRR19973071.383000/150‑1 (MQ=60)
                                                                                                                   TACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTG                                >  SRR19973071.905256/1‑150 (MQ=60)
                                                                                                                   TACCAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTG                                >  SRR19973071.1298961/1‑150 (MQ=60)
                                                                                                                    ACAAAATCCATCTCTTTACCCATGGCTTTAATAGTAGACTCGGCCATCAAGCGCATCTTGTGCGGCCTTTGATGCAAGAGAAAAAACCCCTTCTCGCTGCCAACCAGGTTGTTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGA                               <  SRR19973071.3616897/150‑1 (MQ=60)
                                                                                                                      CAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGC                             >  SRR19973071.1045227/1‑150 (MQ=60)
                                                                                                                      CAAATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGC                             <  SRR19973071.3922708/150‑1 (MQ=60)
                                                                                                                        AATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCG                           >  SRR19973071.1174719/1‑150 (MQ=60)
                                                                                                                         ATCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGA                          >  SRR19973071.1006718/1‑150 (MQ=60)
                                                                                                                          TCCATCGCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAG                         >  SRR19973071.2132152/1‑150 (MQ=60)
                                                                                                                                GCATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATC                   >  SRR19973071.1963733/1‑150 (MQ=60)
                                                                                                                                GCATTACCCAGGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATC                   <  SRR19973071.4224415/150‑1 (MQ=60)
                                                                                                                                 CATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCC                  >  SRR19973071.4709263/1‑150 (MQ=60)
                                                                                                                                 CATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCC                  <  SRR19973071.3599772/150‑1 (MQ=60)
                                                                                                                                  ATTACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCG                 <  SRR19973071.2256051/150‑1 (MQ=60)
                                                                                                                                   TTACCCAGGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGT                <  SRR19973071.958943/150‑1 (MQ=60)
                                                                                                                                    TACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTT               <  SRR19973071.1485964/150‑1 (MQ=60)
                                                                                                                                    TACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTT               >  SRR19973071.1076500/1‑150 (MQ=60)
                                                                                                                                    TACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTT               <  SRR19973071.4767199/150‑1 (MQ=60)
                                                                                                                                    TACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTT               >  SRR19973071.54379/1‑150 (MQ=60)
                                                                                                                                    TACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTT               <  SRR19973071.4767530/150‑1 (MQ=60)
                                                                                                                                     ACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTT              >  SRR19973071.717727/1‑150 (MQ=60)
                                                                                                                                     ACCCATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTT              <  SRR19973071.3026376/150‑1 (MQ=60)
                                                                                                                                        CATGGCGGTAATAGGAGACTCGGCCATCAATCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCAGTTTAAT           >  SRR19973071.2708441/1‑150 (MQ=60)
                                                                                                                                        CATGGCGGTAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAAT           >  SRR19973071.2708270/1‑150 (MQ=60)
                                                                                                                                           GGCGGTAATAGGAGACTCGGCCACCAAGCGCATCATCTGCGGCCAGGGATGCCAGAGAAAAAACCCCGGCACGCTGCCAACCCGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCC        <  SRR19973071.942757/150‑1 (MQ=60)
                                                                                                                                                TAATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTA   <  SRR19973071.3887664/150‑1 (MQ=60)
                                                                                                                                                 AATAGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG  >  SRR19973071.888182/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
CACGAATATCCTGCCGTGACATACGCAGCTTTTTCAGGTGGCTGAATATTTGGAAAAAGACGTCAAATCCCACCATTGGAATGACACCAAGCACCACCAGCAGTGCGCATAGCCCTACCAAATCCATCGCATTACCCATGGCGGTAATCGGAGACTCGGCCATCAAGCGCATCATCTGCGGCCAGTGATGCCAGAGAAAAAACCCCGTCACGCTGCCAACCAGGATGGTTTTCAAAATTGCTTTAAGCAACTCCGCGCCAGTCTGAGCCGAGAACATCCGTTTAATGCCCGGTAG  >  CP009273/1959767‑1960061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑