Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,442,770 A→C 15.0% D253E (GAT→GAG)  yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,442,7700AC15.0% 623.2 / 119.3 266D253E (GAT→GAG) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (114/112);  new base C (23/17);  total (137/129)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTG  >  CP009273/2442621‑2442919
                                                                                                                                                     |                                                                                                                                                     
  cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATc                                                                                                                                                     <  2:3611222/150‑1 (MQ=255)
  cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATc                                                                                                                                                     <  2:234183/150‑1 (MQ=255)
   ggAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCa                                                                                                                                                    >  1:56830/1‑150 (MQ=255)
     aGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGGACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAac                                                                                                                                                  <  1:4476386/150‑1 (MQ=255)
       ttGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAacac                                                                                                                                                <  1:1280587/150‑1 (MQ=255)
       ttGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAacac                                                                                                                                                <  2:4461419/150‑1 (MQ=255)
                  agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                                                                                                     >  1:1059958/1‑150 (MQ=255)
                   gTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCatat                                                                                                                                    >  1:1370914/1‑150 (MQ=255)
                       aGCGCGTTGCGCCTGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgtg                                                                                                                                <  2:4290958/150‑1 (MQ=255)
                                   cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTc                                                                                                                    <  2:405165/150‑1 (MQ=255)
                                   cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTc                                                                                                                    <  2:2693731/150‑1 (MQ=255)
                                   cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTc                                                                                                                    <  1:4396263/150‑1 (MQ=255)
                                   cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTc                                                                                                                    <  1:4541256/150‑1 (MQ=255)
                                    aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTcc                                                                                                                   <  1:2205971/150‑1 (MQ=255)
                                    aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTcc                                                                                                                   >  1:4092992/1‑150 (MQ=255)
                                    aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTca                                                                                                                   >  2:3608003/1‑149 (MQ=255)
                                     ggTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCg                                                                                                                  >  1:2984686/1‑150 (MQ=255)
                                     ggTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCg                                                                                                                  >  1:4370833/1‑150 (MQ=255)
                                       tACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTTTAACTGGGTGTCCGtg                                                                                                                >  2:1145559/1‑149 (MQ=255)
                                         cGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtat                                                                                                              <  1:1484485/150‑1 (MQ=255)
                                         cGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtat                                                                                                              <  1:2299871/150‑1 (MQ=255)
                                         cGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtat                                                                                                              <  1:1484228/150‑1 (MQ=255)
                                          gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                                                                                                             >  1:2064772/1‑150 (MQ=255)
                                                 ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTc                                                                                                      >  2:4093477/1‑150 (MQ=255)
                                                 ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTAGAGGGTGTc                                                                                                      >  2:4135450/1‑150 (MQ=255)
                                                    aGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAt                                                                                                   >  1:3667427/1‑150 (MQ=255)
                                                     ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                                                                                                  <  2:1012848/150‑1 (MQ=255)
                                                      gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAttt                                                                                                 <  2:1420308/150‑1 (MQ=255)
                                                      gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAttt                                                                                                 <  1:1177739/150‑1 (MQ=255)
                                                         cGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGc                                                                                              <  1:502900/150‑1 (MQ=255)
                                                          gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTTGCg                                                                                             >  2:2997707/1‑150 (MQ=255)
                                                          gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCg                                                                                             >  2:4321223/1‑150 (MQ=255)
                                                          gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCg                                                                                             >  2:2497635/1‑150 (MQ=255)
                                                            aTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTg                                                                                           >  1:2157234/1‑150 (MQ=255)
                                                                cccTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg                                                                                       >  1:2705698/1‑150 (MQ=255)
                                                                 cctgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCga                                                                                      <  1:315589/150‑1 (MQ=255)
                                                                   tgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCgaga                                                                                    <  1:2868830/150‑1 (MQ=255)
                                                                   tgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCgaga                                                                                    >  1:3967030/1‑150 (MQ=255)
                                                                    gcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc                                                                                   <  1:4347277/150‑1 (MQ=255)
                                                                      ctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACtt                                                                                 >  2:1029871/1‑150 (MQ=255)
                                                                             aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                          >  1:2520018/1‑150 (MQ=255)
                                                                             aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                          >  1:3129780/1‑150 (MQ=255)
                                                                             aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                          <  2:1345591/150‑1 (MQ=255)
                                                                              tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTg                                                                         <  2:764480/150‑1 (MQ=255)
                                                                              tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTg                                                                         <  2:3814447/150‑1 (MQ=255)
                                                                               cTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGt                                                                        <  1:4144416/150‑1 (MQ=255)
                                                                                    ggCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACAt                                                                   <  1:195347/150‑1 (MQ=255)
                                                                                      cTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATcc                                                                 >  2:2641122/1‑150 (MQ=255)
                                                                                       tACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATccc                                                                <  1:1707324/150‑1 (MQ=255)
                                                                                        aCAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCg                                                               <  1:656991/150‑1 (MQ=255)
                                                                                        aCAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCg                                                               <  1:2936175/150‑1 (MQ=255)
                                                                                         cAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGa                                                              >  1:4401687/1‑150 (MQ=255)
                                                                                             tCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAcgcg                                                          <  2:3632585/150‑1 (MQ=255)
                                                                                                ttCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTTTAGGGTGTCGATTTGGCGTGACCGTGACTTCCGCCTTGTCACAGCGCGACggggga                                                       >  2:2798956/1‑148 (MQ=255)
                                                                                                   aGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTaaaa                                                    >  2:1073502/1‑150 (MQ=255)
                                                                                                     tATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGt                                                  >  1:695521/1‑150 (MQ=255)
                                                                                                       tGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTa                                                >  2:1584494/1‑150 (MQ=255)
                                                                                                        gtccTGGAACCGGCAAGTAGAATCGCTTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTat                                               <  2:3994273/147‑1 (MQ=255)
                                                                                                        gTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTat                                               <  1:3183286/150‑1 (MQ=255)
                                                                                                          aCTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatca                                             >  2:775675/1‑150 (MQ=255)
                                                                                                          aCTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatca                                             <  2:314902/150‑1 (MQ=255)
                                                                                                          aCTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCAGATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatca                                             <  2:3150624/150‑1 (MQ=255)
                                                                                                            tGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatcatc                                           >  2:1173376/1‑150 (MQ=255)
                                                                                                            tGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatcatc                                           <  2:2157234/150‑1 (MQ=255)
                                                                                                            tGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatcatc                                           >  2:623679/1‑150 (MQ=255)
                                                                                                             ggAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCt                                          <  1:4352550/150‑1 (MQ=255)
                                                                                                             ggAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCt                                          >  2:3345115/1‑150 (MQ=255)
                                                                                                                aCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTTTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtgtg                                       <  2:1753039/150‑1 (MQ=255)
                                                                                                                 ccGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGc                                      >  1:1249270/1‑150 (MQ=255)
                                                                                                                  cGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCa                                     >  2:4487094/1‑150 (MQ=255)
                                                                                                                      aaGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAAccc                                 >  1:4042092/1‑150 (MQ=255)
                                                                                                                          aGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCg                             <  2:375332/150‑1 (MQ=255)
                                                                                                                             aTCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCa                          >  1:168334/1‑150 (MQ=255)
                                                                                                                             aTCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCa                          <  1:1510010/150‑1 (MQ=255)
                                                                                                                             aTCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCAGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCa                          >  2:3257413/1‑150 (MQ=255)
                                                                                                                               cGATTGGCTGCATACTGCTGGTATCAACACGCACGACCAGATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGt                        <  2:2559097/150‑1 (MQ=255)
                                                                                                                                 aTTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTaa                      <  1:4211641/150‑1 (MQ=255)
                                                                                                                                 aTTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTaa                      <  2:1062679/150‑1 (MQ=255)
                                                                                                                                   tGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacc                    <  2:369763/150‑1 (MQ=255)
                                                                                                                                   tGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacc                    <  1:3978892/150‑1 (MQ=255)
                                                                                                                                   tGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGGTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacc                    >  1:177360/1‑150 (MQ=255)
                                                                                                                                     gCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAaccac                  >  2:1931390/1‑150 (MQ=255)
                                                                                                                                       tGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt                <  1:516036/150‑1 (MQ=255)
                                                                                                                                       tGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt                >  1:1879879/1‑150 (MQ=255)
                                                                                                                                         cATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGt              >  2:1670842/1‑150 (MQ=255)
                                                                                                                                         cATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGt              >  1:1530430/1‑150 (MQ=255)
                                                                                                                                           tACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGttt            >  2:1682560/1‑150 (MQ=255)
                                                                                                                                           tACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGttt            <  2:1135202/150‑1 (MQ=255)
                                                                                                                                            aCTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttc           >  1:2353476/1‑150 (MQ=255)
                                                                                                                                             ctgctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttca          >  2:3619639/1‑150 (MQ=255)
                                                                                                                                              tgctgGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcat         >  2:931170/1‑150 (MQ=255)
                                                                                                                                              tgctgGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcat         >  2:3044135/1‑150 (MQ=255)
                                                                                                                                              tgctgGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcat         >  1:2838501/1‑150 (MQ=255)
                                                                                                                                               gctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcatt        <  2:4089933/150‑1 (MQ=255)
                                                                                                                                                ctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcattc       <  2:3573373/150‑1 (MQ=255)
                                                                                                                                                ctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcattc       <  2:3572518/150‑1 (MQ=255)
                                                                                                                                                   gTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATc    >  2:670272/1‑150 (MQ=255)
                                                                                                                                                   gTATCAAAACGCACGACCATATAGTGTAACTCTTTGTCCGTTATAGGCTGTAGATTTGGCGTGACCGATACTTCCGACTAGTCACATCACGACGAGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAAACACCTGTATCATTCCTc    >  2:835967/1‑150 (MQ=255)
                                                                                                                                                    tATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCt   >  1:1139622/1‑150 (MQ=255)
                                                                                                                                                     aTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTg  >  1:881413/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTG  >  CP009273/2442621‑2442919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A