Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,442,770 A→C 40.5% D253E (GAT→GAG)  yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,442,7700AC40.5% 67.1 / inf 220D253E (GAT→GAG) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (56/75);  new base C (46/43);  total (102/118)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 223 total aligned reads displayed.

GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  >  CP009273/2442621‑2442918
                                                                                                                                                     |                                                                                                                                                    
  cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATc                                                                                                                                                    >  2:3046015/1‑150 (MQ=255)
     aGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCCAGGAGAATCGATTGGCTGCATACTGCTGGTATCAac                                                                                                                                                 >  1:1073953/1‑150 (MQ=255)
       ttGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAacac                                                                                                                                               <  1:1020434/150‑1 (MQ=255)
        tGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAAcacg                                                                                                                                              <  1:4144818/150‑1 (MQ=255)
           ccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgcac                                                                                                                                           <  1:1442793/150‑1 (MQ=255)
           ccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAAGGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgcac                                                                                                                                           <  1:261827/150‑1 (MQ=255)
            cTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgcacg                                                                                                                                          >  2:4052417/1‑150 (MQ=255)
                ggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                                                                                                      <  2:3346078/150‑1 (MQ=255)
                  agTTGAGCTCGTTGCGCCAGTTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCATCACGCACGACCata                                                                                                                                    <  1:572666/150‑1 (MQ=255)
                  agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCata                                                                                                                                    <  2:2209654/150‑1 (MQ=255)
                   gTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCatat                                                                                                                                   <  2:1777251/150‑1 (MQ=255)
                     tGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCg                                                                                                                                 >  2:1293768/1‑150 (MQ=255)
                      gAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAgt                                                                                                                                >  1:609121/1‑150 (MQ=255)
                       aGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAgtg                                                                                                                               <  2:1406563/150‑1 (MQ=255)
                       aGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAgtg                                                                                                                               <  1:4161274/150‑1 (MQ=255)
                       aGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgtg                                                                                                                               <  2:976770/150‑1 (MQ=255)
                         cgcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTa                                                                                                                             <  1:3236759/150‑1 (MQ=255)
                           cgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACACACGACCATATAGTGTAAc                                                                                                                           >  2:3489861/1‑150 (MQ=255)
                            gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACt                                                                                                                          >  2:4151825/1‑150 (MQ=255)
                               gcgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGtt                                                                                                                       <  2:879914/150‑1 (MQ=255)
                                cgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGttt                                                                                                                      <  1:3920771/150‑1 (MQ=255)
                                 gcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTg                                                                                                                     <  1:2748133/150‑1 (MQ=255)
                                  ccAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGt                                                                                                                    >  1:3111021/1‑150 (MQ=255)
                                    aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTcc                                                                                                                  >  1:1606426/1‑150 (MQ=255)
                                    aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTcc                                                                                                                  <  2:3002284/150‑1 (MQ=255)
                                          gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTtata                                                                                                            <  2:1180064/150‑1 (MQ=255)
                                            gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAgg                                                                                                          >  2:4111538/1‑150 (MQ=255)
                                             tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAggg                                                                                                         <  2:2105087/150‑1 (MQ=255)
                                                 ggCAGGGGCGAATGACCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTc                                                                                                     <  2:1805768/150‑1 (MQ=255)
                                                  gCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGGGATAGGGTGTCg                                                                                                    >  2:3684362/1‑150 (MQ=255)
                                                   cAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGa                                                                                                   <  1:3965947/150‑1 (MQ=255)
                                                    aGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGAt                                                                                                  <  2:382764/150‑1 (MQ=255)
                                                    aGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTACGTTATAGGGTGTCGAt                                                                                                  >  1:1308961/1‑150 (MQ=255)
                                                     ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                                                                                                 <  1:1188691/150‑1 (MQ=255)
                                                     ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGAtt                                                                                                 <  1:3386435/150‑1 (MQ=255)
                                                      gggCTAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGAttt                                                                                                >  1:4059906/1‑150 (MQ=255)
                                                          gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCg                                                                                            >  1:1525830/1‑150 (MQ=255)
                                                          gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCg                                                                                            >  1:3622172/1‑150 (MQ=255)
                                                           aaTGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGt                                                                                           >  1:2685665/1‑150 (MQ=255)
                                                             tGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGa                                                                                         >  1:2252053/1‑150 (MQ=255)
                                                               ccccTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGAcc                                                                                       <  1:2187534/150‑1 (MQ=255)
                                                                cccTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg                                                                                      >  2:484505/1‑150 (MQ=255)
                                                                  ctgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCgag                                                                                    <  1:611765/150‑1 (MQ=255)
                                                                    gcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc                                                                                  <  2:330555/150‑1 (MQ=255)
                                                                    gcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc                                                                                  <  1:2477765/150‑1 (MQ=255)
                                                                      ctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACtt                                                                                <  1:1515963/150‑1 (MQ=255)
                                                                      ctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACtt                                                                                >  1:1863901/1‑150 (MQ=255)
                                                                       tgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTc                                                                               <  2:652648/150‑1 (MQ=255)
                                                                        ggTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTcc                                                                              >  2:3290621/1‑150 (MQ=255)
                                                                         gtgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCg                                                                             <  1:218107/150‑1 (MQ=255)
                                                                         gtgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCg                                                                             >  2:739689/1‑150 (MQ=255)
                                                                          tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGc                                                                            >  2:2212259/1‑150 (MQ=255)
                                                                           gtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGcc                                                                           >  1:62269/1‑150 (MQ=255)
                                                                             aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                         >  1:3296442/1‑150 (MQ=255)
                                                                             aTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCtt                                                                         >  1:2672663/1‑150 (MQ=255)
                                                                                 aaGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTca                                                                     >  1:3090950/1‑150 (MQ=255)
                                                                                 aaGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTca                                                                     <  2:1842736/150‑1 (MQ=255)
                                                                                        aCAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCg                                                              <  2:195295/150‑1 (MQ=255)
                                                                                         cAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGa                                                             >  2:4050723/1‑150 (MQ=255)
                                                                                          aaCTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAc                                                            >  1:3255966/1‑150 (MQ=255)
                                                                                           aCTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAcg                                                           >  2:2689306/1‑150 (MQ=255)
                                                                                           aCTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATGGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAcg                                                           <  2:1060445/150‑1 (MQ=255)
                                                                                               aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                       <  1:2871582/150‑1 (MQ=255)
                                                                                               aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                       <  1:2871557/150‑1 (MQ=255)
                                                                                               aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                       <  1:301345/150‑1 (MQ=255)
                                                                                               aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                       <  1:3828527/150‑1 (MQ=255)
                                                                                               aTTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGt                                                       >  2:3966535/1‑150 (MQ=255)
                                                                                                        gTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGGTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTCAAAGTTat                                              >  2:2922862/1‑150 (MQ=255)
                                                                                                        gTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTat                                              >  2:2919916/1‑150 (MQ=255)
                                                                                                            tGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTatcatc                                          <  1:1786625/150‑1 (MQ=255)
                                                                                                             ggAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGNCACATCCCGACGCGGTAAAAGTTATCATCt                                         <  1:3855786/150‑1 (MQ=255)
                                                                                                             ggAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGNCACATCCCGACGCGGTAAAAGTTATCATCt                                         <  1:3855897/150‑1 (MQ=255)
                                                                                                               aaCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtgt                                       >  2:2019442/1‑150 (MQ=255)
                                                                                                                aCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCtgtg                                      >  2:2336474/1‑150 (MQ=255)
                                                                                                                 ccGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGc                                     >  1:3730969/1‑150 (MQ=255)
                                                                                                                  cGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCa                                    >  1:1996653/1‑150 (MQ=255)
                                                                                                                  cGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCa                                    >  1:1996644/1‑150 (MQ=255)
                                                                                                                  cGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCa                                    >  1:1938848/1‑150 (MQ=255)
                                                                                                                      aaGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAAccc                                >  1:3543415/1‑150 (MQ=255)
                                                                                                                       aGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCa                               >  1:2394103/1‑150 (MQ=255)
                                                                                                                       aGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCa                               <  2:1525938/150‑1 (MQ=255)
                                                                                                                       aGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGAAACCCa                               >  2:3919242/1‑150 (MQ=255)
                                                                                                                       aGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCa                               >  2:81740/1‑150 (MQ=255)
                                                                                                                                gATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTa                      >  2:1056063/1‑150 (MQ=255)
                                                                                                                                  ttGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAac                    <  2:593004/150‑1 (MQ=255)
                                                                                                                                    ggCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAacca                  >  1:3124739/1‑150 (MQ=255)
                                                                                                                                     gCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTACAAGTTATCATCTGTGCAACCCAGCGGCAGTAaccac                 >  2:1428130/1‑150 (MQ=255)
                                                                                                                                      cTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAaccacc                >  1:3048100/1‑150 (MQ=255)
                                                                                                                                       tGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt               >  2:93227/1‑150 (MQ=255)
                                                                                                                                       tGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCt               <  1:3298836/150‑1 (MQ=255)
                                                                                                                                        gCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTg              <  1:2565131/150‑1 (MQ=255)
                                                                                                                                        gCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTg              <  1:341138/150‑1 (MQ=255)
                                                                                                                                            aCTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttc          >  2:2508981/1‑150 (MQ=255)
                                                                                                                                                ctgGTCTCAACACGCACGACCATATAGTGTACCTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcattc      >  2:3251377/1‑150 (MQ=255)
                                                                                                                                                ctgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcattc      <  1:1223149/150‑1 (MQ=255)
                                                                                                                                                 tgGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTttcattca     <  2:2995362/150‑1 (MQ=255)
                                                                                                                                                  ggTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTtcattcat    <  2:2626274/150‑1 (MQ=255)
                                                                                                                                                  ggTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTtcattcat    <  2:2118384/150‑1 (MQ=255)
                                                                                                                                                  ggTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTtcattcat    <  2:118137/150‑1 (MQ=255)
                                                                                                                                                    tATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCt  <  1:90394/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  >  CP009273/2442621‑2442918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  >  CP009273/2442621‑2442918
                                                                                                                                                     |                                                                                                                                                    
GCCGGTGTTGCCCTGAGGTGTTGTGCGCGTTGCGCCAGGTACGGGTAATTGGAGGGGCGTATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTA                                                                                                                                                      <  SRR19973074.4165911/150‑1 (MQ=60)
 CCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCT                                                                                                                                                     >  SRR19973074.499700/1‑150 (MQ=60)
 CCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCT                                                                                                                                                     <  SRR19973074.2132692/150‑1 (MQ=60)
  CGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTC                                                                                                                                                    <  SRR19973074.4050543/150‑1 (MQ=60)
    GAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAA                                                                                                                                                  <  SRR19973074.623411/150‑1 (MQ=60)
     AGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCCAGGAGAATCGATTGGCTGCATACTGCTGGTATCAAC                                                                                                                                                 >  SRR19973074.1075959/1‑150 (MQ=60)
       TTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACAC                                                                                                                                               <  SRR19973074.1022325/150‑1 (MQ=60)
        TGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACG                                                                                                                                              <  SRR19973074.4152456/150‑1 (MQ=60)
        TGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACG                                                                                                                                              <  SRR19973074.4152413/150‑1 (MQ=60)
           CCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCAC                                                                                                                                           <  SRR19973074.1445415/150‑1 (MQ=60)
           CCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCAC                                                                                                                                           <  SRR19973074.1445488/150‑1 (MQ=60)
           CCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAAGGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCAC                                                                                                                                           <  SRR19973074.262343/150‑1 (MQ=60)
           CCTGAGGAGTTGAGCGCGGTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCAC                                                                                                                                           <  SRR19973074.262371/150‑1 (MQ=60)
            CTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACG                                                                                                                                          >  SRR19973074.233899/1‑150 (MQ=60)
             TGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGA                                                                                                                                         <  SRR19973074.3139519/150‑1 (MQ=60)
                  AGTTGAGCTCGTTGCGCCAGTTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCATCACGCACGACCATA                                                                                                                                    <  SRR19973074.573784/150‑1 (MQ=60)
                  AGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATA                                                                                                                                    >  SRR19973074.1479565/1‑150 (MQ=60)
                   GTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATAT                                                                                                                                   <  SRR19973074.2457389/150‑1 (MQ=60)
                   GTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATAT                                                                                                                                   <  SRR19973074.2457423/150‑1 (MQ=60)
                   GTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATAT                                                                                                                                   <  SRR19973074.13739/150‑1 (MQ=60)
                    TTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTACAATCCATTGGCTGCAAACACCTCGTCTCAACACGCACGACCATATA                                                                                                                                  <  SRR19973074.3849957/150‑1 (MQ=60)
                      GAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGT                                                                                                                                >  SRR19973074.610354/1‑150 (MQ=60)
                      GAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGT                                                                                                                                >  SRR19973074.610277/1‑150 (MQ=60)
                      GAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACCGCTGGTATCAACACGCACGACCATATCGT                                                                                                                                >  SRR19973074.165582/1‑150 (MQ=60)
                       AGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTG                                                                                                                               <  SRR19973074.4168929/150‑1 (MQ=60)
                         CGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTA                                                                                                                             <  SRR19973074.3242909/150‑1 (MQ=60)
                         CGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTA                                                                                                                             <  SRR19973074.3242749/150‑1 (MQ=60)
                          GCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGTCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAA                                                                                                                            <  SRR19973074.1874942/150‑1 (MQ=60)
                                CGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTT                                                                                                                      <  SRR19973074.3927981/150‑1 (MQ=60)
                                 GCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTACCTGTTTG                                                                                                                     >  SRR19973074.363342/1‑150 (MQ=60)
                                 GCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTG                                                                                                                     <  SRR19973074.2753195/150‑1 (MQ=60)
                                 GCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTG                                                                                                                     <  SRR19973074.2753036/150‑1 (MQ=60)
                                  CCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGT                                                                                                                    >  SRR19973074.3116730/1‑150 (MQ=60)
                                    AGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCC                                                                                                                  <  SRR19973074.3311743/150‑1 (MQ=60)
                                    AGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCC                                                                                                                  >  SRR19973074.1609485/1‑150 (MQ=60)
                                            GTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGG                                                                                                          >  SRR19973074.595978/1‑150 (MQ=60)
                                             TAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGG                                                                                                         <  SRR19973074.3755105/150‑1 (MQ=60)
                                             TAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGG                                                                                                         <  SRR19973074.3755151/150‑1 (MQ=60)
                                                TGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGT                                                                                                      <  SRR19973074.2783232/150‑1 (MQ=60)
                                                   CAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGA                                                                                                   <  SRR19973074.3973259/150‑1 (MQ=60)
                                                    AGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTACGTTATAGGGTGTCGAT                                                                                                  >  SRR19973074.1311427/1‑150 (MQ=60)
                                                     GGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATT                                                                                                 <  SRR19973074.1190959/150‑1 (MQ=60)
                                                     GGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATT                                                                                                 <  SRR19973074.3392703/150‑1 (MQ=60)
                                                      GGGCTAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTT                                                                                                >  SRR19973074.4067415/1‑150 (MQ=60)
                                                          GAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCG                                                                                            >  SRR19973074.3628865/1‑150 (MQ=60)
                                                          GAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCG                                                                                            >  SRR19973074.1528712/1‑150 (MQ=60)
                                                          GAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCG                                                                                            >  SRR19973074.2546561/1‑150 (MQ=60)
                                                           AATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGT                                                                                           >  SRR19973074.2690655/1‑150 (MQ=60)
                                                             TGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGA                                                                                         >  SRR19973074.2256285/1‑150 (MQ=60)
                                                               CCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACC                                                                                       <  SRR19973074.3108891/150‑1 (MQ=60)
                                                               CCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACC                                                                                       <  SRR19973074.2191639/150‑1 (MQ=60)
                                                                  CTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAG                                                                                    <  SRR19973074.612926/150‑1 (MQ=60)
                                                                    GCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAC                                                                                  <  SRR19973074.2482362/150‑1 (MQ=60)
                                                                      CTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTT                                                                                <  SRR19973074.1518825/150‑1 (MQ=60)
                                                                      CTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTT                                                                                <  SRR19973074.1518830/150‑1 (MQ=60)
                                                                      CTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTT                                                                                >  SRR19973074.1867463/1‑150 (MQ=60)
                                                                        GGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCC                                                                              >  SRR19973074.3391427/1‑150 (MQ=60)
                                                                         GTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCG                                                                             <  SRR19973074.218555/150‑1 (MQ=60)
                                                                         GTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCG                                                                             <  SRR19973074.218560/150‑1 (MQ=60)
                                                                         GTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCG                                                                             <  SRR19973074.3958805/150‑1 (MQ=60)
                                                                           GTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCC                                                                           >  SRR19973074.62430/1‑150 (MQ=60)
                                                                             ATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTT                                                                         >  SRR19973074.2677821/1‑150 (MQ=60)
                                                                             ATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTT                                                                         >  SRR19973074.2677638/1‑150 (MQ=60)
                                                                             ATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTT                                                                         >  SRR19973074.3302558/1‑150 (MQ=60)
                                                                              TCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTG                                                                        <  SRR19973074.438606/150‑1 (MQ=60)
                                                                              TCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTG                                                                        <  SRR19973074.438519/150‑1 (MQ=60)
                                                                                 GAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCA                                                                     >  SRR19973074.3959393/1‑150 (MQ=60)
                                                                                 AAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCA                                                                     >  SRR19973074.3096628/1‑150 (MQ=60)
                                                                                    GGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACAT                                                                  >  SRR19973074.3627051/1‑150 (MQ=60)
                                                                                     GCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATC                                                                 >  SRR19973074.208256/1‑150 (MQ=60)
                                                                                          AACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGAC                                                            >  SRR19973074.3261992/1‑150 (MQ=60)
                                                                                              CATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCACATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGG                                                        <  SRR19973074.1630576/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.2192821/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.2876885/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.2876888/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.2876910/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.301910/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.301908/150‑1 (MQ=60)
                                                                                               ATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGT                                                       <  SRR19973074.3835508/150‑1 (MQ=60)
                                                                                                    GTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAG                                                  <  SRR19973074.3432955/150‑1 (MQ=60)
                                                                                                        GTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTAT                                              >  SRR19973074.2600163/1‑150 (MQ=60)
                                                                                                            TGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATC                                          <  SRR19973074.1790010/150‑1 (MQ=60)
                                                                                                            TGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATC                                          <  SRR19973074.1290949/150‑1 (MQ=60)
                                                                                                             GGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGNCACATCCCGACGCGGTAAAAGTTATCATCT                                         <  SRR19973074.3862931/150‑1 (MQ=60)
                                                                                                             GGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGNCACATCCCGACGCGGTAAAAGTTATCATCT                                         <  SRR19973074.3862821/150‑1 (MQ=60)
                                                                                                             GGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGNCACATCCCGACGCGGTAAAAGTTATCATCT                                         <  SRR19973074.3862820/150‑1 (MQ=60)
                                                                                                              GAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTG                                        >  SRR19973074.3914959/1‑150 (MQ=60)
                                                                                                                 CCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGC                                     >  SRR19973074.3737845/1‑150 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    >  SRR19973074.2000430/1‑150 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    >  SRR19973074.2000440/1‑150 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    >  SRR19973074.2000436/1‑150 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    >  SRR19973074.1942553/1‑150 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    <  SRR19973074.1795809/150‑1 (MQ=60)
                                                                                                                  CGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCA                                    >  SRR19973074.1582538/1‑150 (MQ=60)
                                                                                                                      AAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCC                                >  SRR19973074.3549943/1‑150 (MQ=60)
                                                                                                                       AGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCA                               >  SRR19973074.2398544/1‑150 (MQ=60)
                                                                                                                       AGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCA                               <  SRR19973074.3199648/150‑1 (MQ=60)
                                                                                                                       AGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCA                               >  SRR19973074.671793/1‑150 (MQ=60)
                                                                                                                             ATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCA                         <  SRR19973074.1175913/150‑1 (MQ=60)
                                                                                                                                    GGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCA                  >  SRR19973074.3130337/1‑150 (MQ=60)
                                                                                                                                    GGCTGCATACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCA                  >  SRR19973074.3130465/1‑150 (MQ=60)
                                                                                                                                      CTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACC                >  SRR19973074.3053753/1‑150 (MQ=60)
                                                                                                                                      CTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACC                >  SRR19973074.3053730/1‑150 (MQ=60)
                                                                                                                                      CTGCATACTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACC                >  SRR19973074.3053158/1‑150 (MQ=60)
                                                                                                                                       TGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCT               <  SRR19973074.3304956/150‑1 (MQ=60)
                                                                                                                                       TGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCT               <  SRR19973074.3304916/150‑1 (MQ=60)
                                                                                                                                        GCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTG              <  SRR19973074.341754/150‑1 (MQ=60)
                                                                                                                                        GCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTG              <  SRR19973074.1223793/150‑1 (MQ=60)
                                                                                                                                        GCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTG              <  SRR19973074.2569919/150‑1 (MQ=60)
                                                                                                                                            ACTGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTC          <  SRR19973074.3277769/150‑1 (MQ=60)
                                                                                                                                             CTGCTGGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCA         <  SRR19973074.291201/150‑1 (MQ=60)
                                                                                                                                              TGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCAT        <  SRR19973074.1070255/150‑1 (MQ=60)
                                                                                                                                              TGCTGGTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCAT        >  SRR19973074.3400950/1‑150 (MQ=60)
                                                                                                                                                CTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTC      <  SRR19973074.1225491/150‑1 (MQ=60)
                                                                                                                                                 TGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCA     <  SRR19973074.981548/150‑1 (MQ=60)
                                                                                                                                                 TGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCA     <  SRR19973074.981554/150‑1 (MQ=60)
                                                                                                                                                  GGTCTCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCAT    >  SRR19973074.1992933/1‑150 (MQ=60)
                                                                                                                                                   GTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATC   <  SRR19973074.3139970/150‑1 (MQ=60)
                                                                                                                                                   GTCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATC   <  SRR19973074.956670/150‑1 (MQ=60)
                                                                                                                                                    TCTCAACACGCACGACCATATAGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  <  SRR19973074.84598/150‑1 (MQ=60)
                                                                                                                                                    TATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  <  SRR19973074.2681137/150‑1 (MQ=60)
                                                                                                                                                    TATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  <  SRR19973074.212102/150‑1 (MQ=60)
                                                                                                                                                    TATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  <  SRR19973074.90617/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCT  >  CP009273/2442621‑2442918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑