Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,346 G→T 92.0% intergenic (‑55/+148) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,3460GT92.0% 605.6 / 41.5 209intergenic (‑55/+148)sixA/fadJphosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (10/7);  new base T (92/100);  total (102/107)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT  >  CP009273/2450199‑2450494
                                                                                                                                                   |                                                                                                                                                    
  aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaag                                                                                                                                                  <  2:3317971/150‑1 (MQ=255)
    tCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaagaa                                                                                                                                                >  2:254014/1‑150 (MQ=255)
    tCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaagaa                                                                                                                                                >  1:2762605/1‑150 (MQ=255)
      gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                              <  1:3345968/150‑1 (MQ=255)
      gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                              >  2:3345020/1‑150 (MQ=255)
      gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                              >  2:1070543/1‑150 (MQ=255)
        cacaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACg                                                                                                                                            <  2:2110931/150‑1 (MQ=255)
          caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCACGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGt                                                                                                                                          >  2:4120682/1‑150 (MQ=255)
           aaCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTg                                                                                                                                         <  2:951919/150‑1 (MQ=255)
                      gTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtgt                                                                                                                              <  1:338324/150‑1 (MQ=255)
                      gTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtgt                                                                                                                              <  2:17788/150‑1 (MQ=255)
                       tCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtgtg                                                                                                                             <  1:3739714/150‑1 (MQ=255)
                        cAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                                            >  2:3311201/1‑150 (MQ=255)
                          gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                                          <  1:2276822/150‑1 (MQ=255)
                            ggACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTa                                                                                                                        <  1:3999746/150‑1 (MQ=255)
                               agAACGGAATCACTGGCTGCATCGAGGGCTGCGTCTCCGTGACGCATGATAAAAACTTGCATATTGCACCTCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGAATTGTGCATTAtac                                                                                                                     <  2:125035/149‑3 (MQ=255)
                                gAACGGAATCACTGGCGGCCTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                                    <  2:1957109/150‑1 (MQ=255)
                                 aaCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAt                                                                                                                   <  1:1223675/150‑1 (MQ=255)
                                      aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                              <  2:2418133/150‑1 (MQ=255)
                                      aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                              <  2:3956262/150‑1 (MQ=255)
                                         cACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGaa                                                                                                           >  2:3238665/1‑150 (MQ=255)
                                          aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                                          <  1:2650313/150‑1 (MQ=255)
                                            tGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTTTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGa                                                                                                        <  2:3142566/150‑1 (MQ=255)
                                            tGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGa                                                                                                        <  2:3652494/150‑1 (MQ=255)
                                             ggcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                                       <  1:1518295/150‑1 (MQ=255)
                                                ggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACg                                                                                                    >  2:3491071/1‑150 (MQ=255)
                                                  cATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCt                                                                                                  >  2:1355171/1‑150 (MQ=255)
                                                    tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                                >  1:593189/1‑150 (MQ=255)
                                                    tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                                >  1:593199/1‑150 (MQ=255)
                                                     cGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                                       <  2:3827220/142‑1 (MQ=255)
                                                     cGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                                       >  1:3827220/1‑142 (MQ=255)
                                                      gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                                              >  2:1238662/1‑150 (MQ=255)
                                                       aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                             <  1:4089404/150‑1 (MQ=255)
                                                        gggCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttttt                                                                                            <  1:2341498/150‑1 (MQ=255)
                                                         ggCTGCGTCGCCGTGACGCATGATCACATCTTGCATAGTGCTCCGCTTGTGTTACCCAGATGCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGGGGCCGGCATGGTGCCTTATCCATTCACCGAATGAACCGCTGtttttt                                                                                           >  1:1122354/1‑150 (MQ=255)
                                                          gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                                          <  2:1650259/150‑1 (MQ=255)
                                                            tGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAcc                                                                                        <  1:1248560/150‑1 (MQ=255)
                                                                  gCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATg                                                                                  <  1:2736416/150‑1 (MQ=255)
                                                                   ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                                 >  1:3078866/1‑150 (MQ=255)
                                                                    cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                                <  2:3960738/150‑1 (MQ=255)
                                                                      tGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCCTTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGt                                                                              >  2:2485950/1‑150 (MQ=255)
                                                                         cGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                                           >  1:3425889/1‑150 (MQ=255)
                                                                            aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                                        <  2:2551541/150‑1 (MQ=255)
                                                                            aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                                        <  2:3262920/150‑1 (MQ=255)
                                                                             tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                                       <  1:3044985/150‑1 (MQ=255)
                                                                             tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                                       <  1:4120695/150‑1 (MQ=255)
                                                                             tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                                       <  1:4120670/150‑1 (MQ=255)
                                                                               aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                     >  1:793568/1‑150 (MQ=255)
                                                                               aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                     >  2:168335/1‑150 (MQ=255)
                                                                               aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                     >  2:167416/1‑150 (MQ=255)
                                                                               aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                     <  1:227524/150‑1 (MQ=255)
                                                                               aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                     >  2:2165114/1‑150 (MQ=255)
                                                                                    aaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAata                                                                <  2:955683/150‑1 (MQ=255)
                                                                                     aCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAatat                                                               >  2:2463028/1‑150 (MQ=255)
                                                                                      catGCATATTGCACCGCTTTTTTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                              <  2:4152402/148‑1 (MQ=255)
                                                                                      cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                              <  1:1150324/150‑1 (MQ=255)
                                                                                      cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                              <  2:4314261/150‑1 (MQ=255)
                                                                                        tGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCAGTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                                            >  2:3435614/1‑150 (MQ=255)
                                                                                            tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                                        <  1:1878849/150‑1 (MQ=255)
                                                                                            tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCTTAAGTATAGTCAATATGCGGTTa                                                        >  2:2504066/1‑150 (MQ=255)
                                                                                             atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                                       <  1:53246/150‑1 (MQ=255)
                                                                                               tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTATACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt                                                     >  1:1419746/1‑150 (MQ=255)
                                                                                                   ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                                 <  1:2948310/150‑1 (MQ=255)
                                                                                                    cGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGTCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGa                                                >  2:1728259/1‑150 (MQ=255)
                                                                                                    cGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGa                                                >  1:3577660/1‑150 (MQ=255)
                                                                                                     gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                               >  1:765893/1‑150 (MQ=255)
                                                                                                      cTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAcc                                              >  2:4083303/1‑150 (MQ=255)
                                                                                                          tGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAaca                                          <  2:2357941/150‑1 (MQ=255)
                                                                                                          tGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAaca                                          <  2:3463654/150‑1 (MQ=255)
                                                                                                           gTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAacac                                         >  2:4021576/1‑150 (MQ=255)
                                                                                                             tAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTc                                       >  1:1708186/1‑150 (MQ=255)
                                                                                                              aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                                      <  2:2078657/150‑1 (MQ=255)
                                                                                                              aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                                      >  1:4089173/1‑150 (MQ=255)
                                                                                                              aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                                      >  1:2103486/1‑150 (MQ=255)
                                                                                                               aCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                                     >  2:2925514/1‑150 (MQ=255)
                                                                                                               aCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                                     <  1:3834205/150‑1 (MQ=255)
                                                                                                                 cAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGcc                                   >  1:1350915/1‑150 (MQ=255)
                                                                                                                 cAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGcc                                   >  2:3473563/1‑150 (MQ=255)
                                                                                                                  aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                                  >  1:986241/1‑150 (MQ=255)
                                                                                                                   gATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAt                                 >  2:875820/1‑150 (MQ=255)
                                                                                                                       cGCCCGATTTCTTTCATTTGAAACCAGATACAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAg                             <  2:3946316/150‑1 (MQ=255)
                                                                                                                           cGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCGGGCATTCTGCCTTATCCATTCACCGAATGAAACGCTGTTTTGTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg                         >  1:3692544/1‑150 (MQ=255)
                                                                                                                           cGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg                         >  1:4427610/1‑150 (MQ=255)
                                                                                                                             ttttCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa                       >  1:2377259/1‑150 (MQ=255)
                                                                                                                             ttttCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa                       >  1:948242/1‑150 (MQ=255)
                                                                                                                               ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt                     <  2:3744651/150‑1 (MQ=255)
                                                                                                                               ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt                     <  1:4532833/150‑1 (MQ=255)
                                                                                                                               ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt                     >  1:266252/1‑150 (MQ=255)
                                                                                                                                  tttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCat                  <  1:4264579/150‑1 (MQ=255)
                                                                                                                                   ttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata                 <  2:2808085/150‑1 (MQ=255)
                                                                                                                                      atttGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAg              >  2:3330998/4‑150 (MQ=255)
                                                                                                                                       tCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGc             >  1:2787497/1‑150 (MQ=255)
                                                                                                                                        ttGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCt            >  2:2331498/2‑150 (MQ=255)
                                                                                                                                           aaaCCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTg         <  1:3491071/150‑1 (MQ=255)
                                                                                                                                            aaCCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGa        >  1:2622816/1‑150 (MQ=255)
                                                                                                                                            aaCCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGa        >  2:653535/1‑150 (MQ=255)
                                                                                                                                              ccAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTTAcc      <  1:4283026/150‑1 (MQ=255)
                                                                                                                                               cAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAGTCATATAGCTTTGACct     >  2:2755982/1‑150 (MQ=255)
                                                                                                                                                  agaaagaaAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttct  >  2:2714937/1‑150 (MQ=255)
                                                                                                                                                  agaaagaaAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttct  >  2:3943696/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT  >  CP009273/2450199‑2450494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450197‑2450495
                                                                                                                                                     |                                                                                                                                                     
CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAT                                                                                                                                                       <  SRR19973076.2948172/150‑1 (MQ=60)
CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAT                                                                                                                                                       <  SRR19973076.2948177/150‑1 (MQ=60)
  AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAA                                                                                                                                                     >  SRR19973076.2711519/1‑150 (MQ=60)
     TTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGA                                                                                                                                                  <  SRR19973076.2279090/150‑1 (MQ=60)
      TCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAA                                                                                                                                                 >  SRR19973076.2766616/1‑150 (MQ=60)
      TCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAA                                                                                                                                                 >  SRR19973076.2766604/1‑150 (MQ=60)
        GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               <  SRR19973076.3350912/150‑1 (MQ=60)
        GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               <  SRR19973076.3350830/150‑1 (MQ=60)
             AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                                          <  SRR19973076.228532/150‑1 (MQ=60)
             AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                                          <  SRR19973076.226876/150‑1 (MQ=60)
                 ATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCG                                                                                                                                      >  SRR19973076.847190/1‑150 (MQ=60)
                        GTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGT                                                                                                                               <  SRR19973076.338846/150‑1 (MQ=60)
                        GTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGT                                                                                                                               <  SRR19973076.2635350/150‑1 (MQ=60)
                         TCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTG                                                                                                                              <  SRR19973076.3745190/150‑1 (MQ=60)
                          CAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                             >  SRR19973076.4012398/1‑150 (MQ=60)
                           AGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCC                                                                                                                            <  SRR19973076.3717853/150‑1 (MQ=60)
                            GAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCT                                                                                                                           <  SRR19973076.2280084/150‑1 (MQ=60)
                              GGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTA                                                                                                                         <  SRR19973076.4005645/150‑1 (MQ=60)
                                   AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                                    >  SRR19973076.2812162/1‑150 (MQ=60)
                                   AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                                    <  SRR19973076.1225487/150‑1 (MQ=60)
                                        AATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACC                                                                                                               >  SRR19973076.1411727/1‑150 (MQ=60)
                                            ACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAT                                                                                                           <  SRR19973076.4204583/150‑1 (MQ=60)
                                            ACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAT                                                                                                           <  SRR19973076.2654169/150‑1 (MQ=60)
                                             CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                                          <  SRR19973076.3429238/150‑1 (MQ=60)
                                               GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                                        <  SRR19973076.1520528/150‑1 (MQ=60)
                                                      TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                                 >  SRR19973076.594105/1‑150 (MQ=60)
                                                      TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                                 >  SRR19973076.594111/1‑150 (MQ=60)
                                                      TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                                 >  SRR19973076.594115/1‑150 (MQ=60)
                                                       CGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAgatcgga                                                                                                >  SRR19973076.3832841/1‑143 (MQ=60)
                                                         AGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTT                                                                                              <  SRR19973076.4095440/150‑1 (MQ=60)
                                                          GGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTT                                                                                             <  SRR19973076.2344866/150‑1 (MQ=60)
                                                          GGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTT                                                                                             <  SRR19973076.2344889/150‑1 (MQ=60)
                                                           GGCTGCGTCGCCGTGACGCATGATCACATCTTGCATAGTGCTCCGCTTGTGTTACCCAGATGCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGGGGCCGGCATGGTGCCTTATCCATTCACCGAATGAACCGCTGTTTTTT                                                                                            >  SRR19973076.1124010/1‑150 (MQ=60)
                                                           GGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTT                                                                                            >  SRR19973076.1124063/1‑150 (MQ=60)
                                                              TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                                         <  SRR19973076.1250409/150‑1 (MQ=60)
                                                              TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                                         <  SRR19973076.1250293/150‑1 (MQ=60)
                                                              TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                                         >  SRR19973076.902799/1‑150 (MQ=60)
                                                                CGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTC                                                                                       <  SRR19973076.1648266/150‑1 (MQ=60)
                                                                    GCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATG                                                                                   <  SRR19973076.2740395/150‑1 (MQ=60)
                                                                     CCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGG                                                                                  >  SRR19973076.3083335/1‑150 (MQ=60)
                                                                     CCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGG                                                                                  >  SRR19973076.3083293/1‑150 (MQ=60)
                                                                      CGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGC                                                                                 <  SRR19973076.1157660/150‑1 (MQ=60)
                                                                         GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                              <  SRR19973076.3307055/150‑1 (MQ=60)
                                                                         GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                              <  SRR19973076.3307859/150‑1 (MQ=60)
                                                                           CGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAG                                                                            >  SRR19973076.3430883/1‑150 (MQ=60)
                                                                           CGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAG                                                                            >  SRR19973076.3430857/1‑150 (MQ=60)
                                                                               TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973076.4126755/150‑1 (MQ=60)
                                                                               TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973076.4126780/150‑1 (MQ=60)
                                                                               TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973076.3047191/150‑1 (MQ=60)
                                                                               TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973076.3049416/150‑1 (MQ=60)
                                                                               CGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973076.4126767/150‑1 (MQ=60)
                                                                                 ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                                      <  SRR19973076.1832031/150‑1 (MQ=60)
                                                                                 ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                                      <  SRR19973076.227914/150‑1 (MQ=60)
                                                                                 ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                                      >  SRR19973076.794658/1‑150 (MQ=60)
                                                                                 ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                                      >  SRR19973076.794737/1‑150 (MQ=60)
                                                                                   AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                                    <  SRR19973076.471602/150‑1 (MQ=60)
                                                                                   AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                                    >  SRR19973076.3895012/1‑150 (MQ=60)
                                                                                        CTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATG                                                               <  SRR19973076.1152023/150‑1 (MQ=60)
                                                                                            CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                                           >  SRR19973076.1476900/1‑150 (MQ=60)
                                                                                              TATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTA                                                         <  SRR19973076.1881552/150‑1 (MQ=60)
                                                                                               ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                                        <  SRR19973076.53362/150‑1 (MQ=60)
                                                                                               ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                                        <  SRR19973076.53341/150‑1 (MQ=60)
                                                                                                 TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                                      >  SRR19973076.3688202/1‑150 (MQ=60)
                                                                                                 TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTATACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                                      >  SRR19973076.1421832/1‑150 (MQ=60)
                                                                                                     CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTG                                                  <  SRR19973076.2952595/150‑1 (MQ=60)
                                                                                                      CGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGA                                                 >  SRR19973076.3582857/1‑150 (MQ=60)
                                                                                                      CGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGA                                                 >  SRR19973076.1887744/1‑150 (MQ=60)
                                                                                                      CGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGA                                                 >  SRR19973076.3773844/1‑150 (MQ=60)
                                                                                                       GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                                >  SRR19973076.767017/1‑150 (MQ=60)
                                                                                                         TTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCA                                              >  SRR19973076.2663540/1‑150 (MQ=60)
                                                                                                               TAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTC                                        >  SRR19973076.1710679/1‑150 (MQ=60)
                                                                                                                AACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                                       >  SRR19973076.2106541/1‑150 (MQ=60)
                                                                                                                AACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                                       >  SRR19973076.4095251/1‑150 (MQ=60)
                                                                                                                AACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                                       >  SRR19973076.4095209/1‑150 (MQ=60)
                                                                                                                 ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                                      <  SRR19973076.3839823/150‑1 (MQ=60)
                                                                                                                 ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                                      <  SRR19973076.3839846/150‑1 (MQ=60)
                                                                                                                   CAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCC                                    >  SRR19973076.1352906/1‑150 (MQ=60)
                                                                                                                    AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                                   >  SRR19973076.987707/1‑150 (MQ=60)
                                                                                                                    AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                                   >  SRR19973076.986424/1‑150 (MQ=60)
                                                                                                                            CCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGC                           <  SRR19973076.1370032/150‑1 (MQ=60)
                                                                                                                            CCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGC                           <  SRR19973076.4106983/150‑1 (MQ=60)
                                                                                                                             CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCGGGCATTCTGCCTTATCCATTCACCGAATGAAACGCTGTTTTGTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973076.3697943/1‑150 (MQ=60)
                                                                                                                             CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973076.2197159/1‑150 (MQ=60)
                                                                                                                             CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973076.4434197/1‑150 (MQ=60)
                                                                                                                             CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGAGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973076.4434218/1‑150 (MQ=60)
                                                                                                                               TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA                        >  SRR19973076.949640/1‑150 (MQ=60)
                                                                                                                               TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA                        >  SRR19973076.2380808/1‑150 (MQ=60)
                                                                                                                               TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA                        >  SRR19973076.2380783/1‑150 (MQ=60)
                                                                                                                               TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA                        >  SRR19973076.2380679/1‑150 (MQ=60)
                                                                                                                                 TTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATT                      <  SRR19973076.4539557/150‑1 (MQ=60)
                                                                                                                                 TTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATT                      >  SRR19973076.266692/1‑150 (MQ=60)
                                                                                                                                  TCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTC                     <  SRR19973076.1294142/150‑1 (MQ=60)
                                                                                                                                   CTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA                    >  SRR19973076.695205/1‑150 (MQ=60)
                                                                                                                                    TTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT                   <  SRR19973076.4270891/150‑1 (MQ=60)
                                                                                                                                    TTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT                   <  SRR19973076.4270882/150‑1 (MQ=60)
                                                                                                                                    TTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT                   <  SRR19973076.4270863/150‑1 (MQ=60)
                                                                                                                                      TCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT                 >  SRR19973076.3546101/1‑150 (MQ=60)
                                                                                                                                       CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA                <  SRR19973076.576468/150‑1 (MQ=60)
                                                                                                                                         TCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC              >  SRR19973076.2791544/1‑150 (MQ=60)
                                                                                                                                         TCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC              >  SRR19973076.2791085/1‑150 (MQ=60)
                                                                                                                                         TCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC              >  SRR19973076.2791545/1‑150 (MQ=60)
                                                                                                                                             AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          <  SRR19973076.3496136/150‑1 (MQ=60)
                                                                                                                                             AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          <  SRR19973076.3642869/150‑1 (MQ=60)
                                                                                                                                             AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          >  SRR19973076.3984204/1‑150 (MQ=60)
                                                                                                                                              AACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGA         >  SRR19973076.2626636/1‑150 (MQ=60)
                                                                                                                                                CCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTTACC       <  SRR19973076.4289353/150‑1 (MQ=60)
                                                                                                                                                CCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACC       <  SRR19973076.4289363/150‑1 (MQ=60)
                                                                                                                                                    ATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT   <  SRR19973076.2427563/150‑1 (MQ=60)
                                                                                                                                                    ATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT   >  SRR19973076.68106/1‑150 (MQ=60)
                                                                                                                                                    ATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT   <  SRR19973076.2427561/150‑1 (MQ=60)
                                                                                                                                                    ATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCT   >  SRR19973076.1901758/1‑150 (MQ=60)
                                                                                                                                                     TAAAGAAATCGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  <  SRR19973076.3032161/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450197‑2450495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑