Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,346 G→T 65.8% intergenic (‑55/+148) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,3460GT65.8% 229.6 / 261.5 225intergenic (‑55/+148)sixA/fadJphosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (31/46);  new base T (87/61);  total (118/107)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 242 total aligned reads displayed.

GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450198‑2450495
                                                                                                                                                    |                                                                                                                                                     
gagaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAgaga                                                                                                                                                      <  2:4763373/150‑1 (MQ=255)
 agaTTCGTCACAACCATTAGTGGTCGGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataa                                                                                                                                                     >  2:1269425/1‑150 (MQ=255)
 agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataa                                                                                                                                                     >  1:2157044/1‑150 (MQ=255)
  gaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaa                                                                                                                                                    <  2:1164096/150‑1 (MQ=255)
   aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaag                                                                                                                                                   <  2:2535191/150‑1 (MQ=255)
       gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                               >  1:1181572/1‑150 (MQ=255)
       gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                               >  1:5100263/1‑150 (MQ=255)
        tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAAc                                                                                                                                              <  2:3727744/150‑1 (MQ=255)
            aaCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTg                                                                                                                                          >  1:3600927/1‑150 (MQ=255)
                aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCg                                                                                                                                      >  1:3403705/1‑150 (MQ=255)
                  tAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGc                                                                                                                                    <  1:1723201/150‑1 (MQ=255)
                   aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCa                                                                                                                                   >  1:3531909/1‑150 (MQ=255)
                      ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtg                                                                                                                                <  2:4418161/150‑1 (MQ=255)
                      ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtg                                                                                                                                <  2:4482322/150‑1 (MQ=255)
                      ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtg                                                                                                                                <  1:1378367/150‑1 (MQ=255)
                         cAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                                             <  1:1440688/150‑1 (MQ=255)
                          agagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGcc                                                                                                                            <  1:1806660/150‑1 (MQ=255)
                               aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                                       <  2:2750150/150‑1 (MQ=255)
                                cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGAAGCATGATAAAAACTTGCATATTTCACCGCTTTTGTTAACCAGCTTCGCCCGTTTTCTTTAATTTGAAACCAGAGAATGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                                      <  1:3059449/150‑1 (MQ=255)
                                 gAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                                     >  2:3752061/1‑150 (MQ=255)
                                    cGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTc                                                                                                                  >  1:2154895/1‑150 (MQ=255)
                                    cGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGTCGGCATTGTGCCTTATCCATTc                                                                                                                  <  2:2700230/150‑1 (MQ=255)
                                       aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                               >  2:666381/1‑150 (MQ=255)
                                        aTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCg                                                                                                              >  2:3654678/1‑150 (MQ=255)
                                          cACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGaa                                                                                                            >  2:4337355/1‑150 (MQ=255)
                                            cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                          <  1:3973102/150‑1 (MQ=255)
                                            cTGGCGGCATCGAGCGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                          <  1:4810434/150‑1 (MQ=255)
                                                cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                                      <  1:3589527/150‑1 (MQ=255)
                                                cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                                      <  2:4604967/150‑1 (MQ=255)
                                                   cATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCt                                                                                                   >  1:4870638/1‑150 (MQ=255)
                                                     tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                                 <  2:2128245/150‑1 (MQ=255)
                                                      cGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtt                                                                                                >  2:772993/1‑150 (MQ=255)
                                                         gggCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttttt                                                                                             <  2:4949009/150‑1 (MQ=255)
                                                            cTGCGTCGCCGTGACGCATGACAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                                          <  1:452723/150‑1 (MQ=255)
                                                             tGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAcc                                                                                         >  2:3639070/1‑150 (MQ=255)
                                                             tGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAcc                                                                                         >  2:865171/1‑150 (MQ=255)
                                                                gTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCa                                                                                      >  1:5170023/1‑150 (MQ=255)
                                                                 tCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCaa                                                                                     >  1:3426157/1‑150 (MQ=255)
                                                                  cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAt                                                                                    <  2:4220411/150‑1 (MQ=255)
                                                                  cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAt                                                                                    <  2:1133658/150‑1 (MQ=255)
                                                                  cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAt                                                                                    <  1:2751165/150‑1 (MQ=255)
                                                                     cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                                 >  2:380665/1‑150 (MQ=255)
                                                                     cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                                 >  2:2766305/1‑150 (MQ=255)
                                                                      gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                                <  1:4683345/150‑1 (MQ=255)
                                                                        gACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTa                                                                              >  2:79820/1‑150 (MQ=255)
                                                                          cGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                                            >  2:3119405/1‑150 (MQ=255)
                                                                          cGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTAATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                                            <  1:277878/150‑1 (MQ=255)
                                                                             aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                                         >  2:1429776/1‑150 (MQ=255)
                                                                              tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                                        >  2:3521397/1‑150 (MQ=255)
                                                                                aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                      <  2:1705817/150‑1 (MQ=255)
                                                                                aTAAAAACGTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                                      <  2:420603/150‑1 (MQ=255)
                                                                                 tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTc                                                                     >  2:1083862/1‑150 (MQ=255)
                                                                                    aaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAat                                                                  <  1:1644467/150‑1 (MQ=255)
                                                                                    aaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAat                                                                  >  1:4040817/1‑150 (MQ=255)
                                                                                           cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                                           >  1:4189279/1‑150 (MQ=255)
                                                                                            atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                                          >  2:2668975/1‑150 (MQ=255)
                                                                                              atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                                        <  1:919135/150‑1 (MQ=255)
                                                                                                 gCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtt                                                     <  1:719071/150‑1 (MQ=255)
                                                                                                    ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                                  >  1:3274504/1‑150 (MQ=255)
                                                                                                     cGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGa                                                 >  2:670427/1‑150 (MQ=255)
                                                                                                      gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                                >  2:1653630/1‑150 (MQ=255)
                                                                                                      gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                                >  1:835068/1‑150 (MQ=255)
                                                                                                       cTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAcc                                               >  1:3815139/1‑150 (MQ=255)
                                                                                                            gTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAacac                                          >  2:719182/1‑150 (MQ=255)
                                                                                                             ttAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGGGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACt                                         <  1:494431/150‑1 (MQ=255)
                                                                                                              tAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTc                                        >  2:590249/1‑150 (MQ=255)
                                                                                                                   aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                                   <  1:2418084/150‑1 (MQ=255)
                                                                                                                     aTTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAtt                                 >  2:3407491/1‑150 (MQ=255)
                                                                                                                     aTTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATTCGTTTACATTTTGACCAACACTCCGCCAtt                                 <  1:4437556/150‑1 (MQ=255)
                                                                                                                      gtCGCCCGGTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGGGCCTGATCCAGTCACCGACTGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTc                                <  2:4836170/149‑1 (MQ=255)
                                                                                                                       tCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCa                               >  2:3347323/1‑150 (MQ=255)
                                                                                                                            cGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg                          >  1:5048920/1‑150 (MQ=255)
                                                                                                                            cGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg                          >  1:3912084/1‑150 (MQ=255)
                                                                                                                             gTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg                         <  1:1269425/150‑1 (MQ=255)
                                                                                                                             gTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg                         >  2:3153214/1‑150 (MQ=255)
                                                                                                                             gTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg                         >  1:4555635/1‑150 (MQ=255)
                                                                                                                             gTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg                         >  2:2335378/1‑150 (MQ=255)
                                                                                                                              ttttCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa                        >  2:4153403/1‑150 (MQ=255)
                                                                                                                               tttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt                       >  2:5171490/1‑150 (MQ=255)
                                                                                                                               tttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt                       <  2:1315393/150‑1 (MQ=255)
                                                                                                                                 tctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTc                     >  1:1128790/1‑150 (MQ=255)
                                                                                                                                  ctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa                    >  2:2844060/1‑150 (MQ=255)
                                                                                                                                  ctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa                    >  2:1222984/1‑150 (MQ=255)
                                                                                                                                    ttcATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata                  >  2:1680351/1‑150 (MQ=255)
                                                                                                                                     tcATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTCACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat                 >  2:906883/1‑150 (MQ=255)
                                                                                                                                     tcATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat                 <  1:330424/150‑1 (MQ=255)
                                                                                                                                       atttGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAg               <  2:2157044/147‑1 (MQ=255)
                                                                                                                                           gAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCttt           <  2:2552676/150‑1 (MQ=255)
                                                                                                                                           gAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCttt           >  1:2294475/1‑150 (MQ=255)
                                                                                                                                           gAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCttt           >  1:1689705/1‑150 (MQ=255)
                                                                                                                                           gAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCttt           <  1:2999678/150‑1 (MQ=255)
                                                                                                                                           gAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCttt           <  1:388867/150‑1 (MQ=255)
                                                                                                                                            aaaCCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTg          >  1:1455821/1‑150 (MQ=255)
                                                                                                                                            aaaCCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTg          <  1:4820240/150‑1 (MQ=255)
                                                                                                                                              aCCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAc        <  1:666381/150‑1 (MQ=255)
                                                                                                                                               ccAGATAAAGAAAAAGGTGGCCGGCATTGTGCATTATCAATTCACCGAATGAAACGCTGTTATTTACATCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAcc       >  2:1847989/1‑150 (MQ=255)
                                                                                                                                                 agatAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCTCCATTCAGCGCGGATTCATATAGCTTTGACctt     <  2:2941227/150‑1 (MQ=255)
                                                                                                                                                 agatAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACctt     <  2:327661/150‑1 (MQ=255)
                                                                                                                                                 agatAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACctt     >  1:2885517/1‑150 (MQ=255)
                                                                                                                                                    gaaagaaaACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACcttctt  >  1:2530796/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450198‑2450495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450199‑2450495
                                                                                                                                                   |                                                                                                                                                     
AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAA                                                                                                                                                     >  SRR19973083.2167866/1‑150 (MQ=60)
      GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               >  SRR19973083.5122016/1‑150 (MQ=60)
      GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               >  SRR19973083.1187430/1‑150 (MQ=60)
      GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               >  SRR19973083.5122027/1‑150 (MQ=60)
      GTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAA                                                                                                                                               <  SRR19973083.1832079/150‑1 (MQ=60)
       TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                              <  SRR19973083.3368951/150‑1 (MQ=60)
        CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACG                                                                                                                                             <  SRR19973083.2273108/150‑1 (MQ=60)
           AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                                          >  SRR19973083.3617391/1‑150 (MQ=60)
           AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                                          >  SRR19973083.3617417/1‑150 (MQ=60)
           AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTG                                                                                                                                          <  SRR19973083.3040536/150‑1 (MQ=60)
              CATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCC                                                                                                                                       >  SRR19973083.3811284/1‑150 (MQ=60)
               ATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCG                                                                                                                                      >  SRR19973083.3419433/1‑150 (MQ=60)
                 TAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGC                                                                                                                                    <  SRR19973083.1731664/150‑1 (MQ=60)
                 TAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGC                                                                                                                                    <  SRR19973083.1731547/150‑1 (MQ=60)
                  AGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCA                                                                                                                                   >  SRR19973083.3548119/1‑150 (MQ=60)
                  AGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCA                                                                                                                                   >  SRR19973083.937096/1‑150 (MQ=60)
                   GTGGTCAGTGGGCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCTTATTGCACCGCTTTTGTTAACCAGCTTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCAT                                                                                                                                  <  SRR19973083.2387080/150‑1 (MQ=60)
                   GTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCAT                                                                                                                                  <  SRR19973083.4977880/150‑1 (MQ=60)
                    TGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATT                                                                                                                                 <  SRR19973083.3449450/150‑1 (MQ=60)
                     GGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTG                                                                                                                                <  SRR19973083.1385219/150‑1 (MQ=60)
                      GTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGT                                                                                                                               <  SRR19973083.3366098/150‑1 (MQ=60)
                       TCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTG                                                                                                                              >  SRR19973083.4497575/1‑150 (MQ=60)
                        CAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                             <  SRR19973083.1447931/150‑1 (MQ=60)
                         AGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCC                                                                                                                            <  SRR19973083.1815592/150‑1 (MQ=60)
                         AGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCC                                                                                                                            <  SRR19973083.1815729/150‑1 (MQ=60)
                            GGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTA                                                                                                                         <  SRR19973083.4045074/150‑1 (MQ=60)
                            GGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTA                                                                                                                         <  SRR19973083.1438248/150‑1 (MQ=60)
                             GACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTAT                                                                                                                        <  SRR19973083.2960309/150‑1 (MQ=60)
                               CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGAAGCATGATAAAAACTTGCATATTTCACCGCTTTTGTTAACCAGCTTCGCCCGTTTTCTTTAATTTGAAACCAGAGAATGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                                      <  SRR19973083.3074112/150‑1 (MQ=60)
                                   CGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTC                                                                                                                  >  SRR19973083.2165710/1‑150 (MQ=60)
                                    GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                                 <  SRR19973083.1356524/150‑1 (MQ=60)
                                     GAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAC                                                                                                                <  SRR19973083.5125677/150‑1 (MQ=60)
                                        TCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGA                                                                                                             <  SRR19973083.4490312/150‑1 (MQ=60)
                                           CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGACTG                                                                                                          >  SRR19973083.3530378/1‑150 (MQ=60)
                                           CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                                          <  SRR19973083.3990253/150‑1 (MQ=60)
                                           CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                                          <  SRR19973083.3990790/150‑1 (MQ=60)
                                           CTGGCGGCATCGAGCGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                                          <  SRR19973083.4831038/150‑1 (MQ=60)
                                            TGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGA                                                                                                         <  SRR19973083.4968520/150‑1 (MQ=60)
                                             GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                                        <  SRR19973083.4856875/150‑1 (MQ=60)
                                             GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                                        <  SRR19973083.4857241/150‑1 (MQ=60)
                                               CGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAC                                                                                                      <  SRR19973083.3605976/150‑1 (MQ=60)
                                                GGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACG                                                                                                     >  SRR19973083.1321729/1‑150 (MQ=60)
                                                GGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACG                                                                                                     >  SRR19973083.4570803/1‑150 (MQ=60)
                                                  CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                                   >  SRR19973083.4891564/1‑150 (MQ=60)
                                                  CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                                   >  SRR19973083.4891553/1‑150 (MQ=60)
                                                   ATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGACTGAAACGCTG                                                                                                  >  SRR19973083.3850607/1‑150 (MQ=60)
                                                      GAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTT                                                                                               >  SRR19973083.1217528/1‑150 (MQ=60)
                                                           CTGCGTCGCCGTGACGCATGACAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAC                                                                                          <  SRR19973083.454788/150‑1 (MQ=60)
                                                            TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                                         <  SRR19973083.282065/150‑1 (MQ=60)
                                                            TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                                         <  SRR19973083.886467/150‑1 (MQ=60)
                                                               GTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCA                                                                                      >  SRR19973083.5191948/1‑150 (MQ=60)
                                                                TCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAA                                                                                     >  SRR19973083.826143/1‑150 (MQ=60)
                                                                TCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAA                                                                                     >  SRR19973083.3441929/1‑150 (MQ=60)
                                                                 CGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAT                                                                                    <  SRR19973083.2764858/150‑1 (MQ=60)
                                                                 CGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAT                                                                                    <  SRR19973083.2764865/150‑1 (MQ=60)
                                                                     GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                                <  SRR19973083.4703453/150‑1 (MQ=60)
                                                                         CGCATGATAAAAACTTGCATATTGCACCGCTTGTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAG                                                                            <  SRR19973083.3300286/150‑1 (MQ=60)
                                                                         CGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTAATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAG                                                                            <  SRR19973083.279342/150‑1 (MQ=60)
                                                                             TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973083.1454918/150‑1 (MQ=60)
                                                                             TGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATA                                                                        <  SRR19973083.1454871/150‑1 (MQ=60)
                                                                               ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                                      >  SRR19973083.975152/1‑150 (MQ=60)
                                                                                TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCCATGGCGTAAGTATAGTC                                                                     >  SRR19973083.777721/1‑150 (MQ=60)
                                                                                   AAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAAT                                                                  >  SRR19973083.4058780/1‑150 (MQ=60)
                                                                                   AAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAAT                                                                  <  SRR19973083.1652657/150‑1 (MQ=60)
                                                                                          CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                                           >  SRR19973083.4207715/1‑150 (MQ=60)
                                                                                             ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                                        <  SRR19973083.923563/150‑1 (MQ=60)
                                                                                                GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                                     <  SRR19973083.4206997/150‑1 (MQ=60)
                                                                                                GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                                     <  SRR19973083.2467703/150‑1 (MQ=60)
                                                                                                GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                                     <  SRR19973083.722632/150‑1 (MQ=60)
                                                                                                 CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTT                                                    <  SRR19973083.3202155/150‑1 (MQ=60)
                                                                                                   CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTG                                                  >  SRR19973083.3289837/1‑150 (MQ=60)
                                                                                                   CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTG                                                  >  SRR19973083.3289935/1‑150 (MQ=60)
                                                                                                    CGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGA                                                 >  SRR19973083.3918047/1‑150 (MQ=60)
                                                                                                     GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                                >  SRR19973083.839026/1‑150 (MQ=60)
                                                                                                      CTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACC                                               >  SRR19973083.3832291/1‑150 (MQ=60)
                                                                                                         TTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAAC                                            <  SRR19973083.2228223/150‑1 (MQ=60)
                                                                                                            TTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                                         <  SRR19973083.4040063/150‑1 (MQ=60)
                                                                                                            TTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGGGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                                         <  SRR19973083.496811/150‑1 (MQ=60)
                                                                                                               ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                                      >  SRR19973083.4667432/1‑150 (MQ=60)
                                                                                                                CCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGC                                     >  SRR19973083.1655956/1‑150 (MQ=60)
                                                                                                                  AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                                   <  SRR19973083.2430500/150‑1 (MQ=60)
                                                                                                                    ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                                 >  SRR19973083.516079/1‑150 (MQ=60)
                                                                                                                    ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                                 <  SRR19973083.2774636/150‑1 (MQ=60)
                                                                                                                    ATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATTCGTTTACATTTTGACCAACACTCCGCCATT                                 <  SRR19973083.4456857/150‑1 (MQ=60)
                                                                                                                    ATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                                 <  SRR19973083.4456826/150‑1 (MQ=60)
                                                                                                                     TTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTC                                >  SRR19973083.3424150/1‑150 (MQ=60)
                                                                                                                         CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG                            <  SRR19973083.2556448/150‑1 (MQ=60)
                                                                                                                         CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG                            >  SRR19973083.4672830/1‑150 (MQ=60)
                                                                                                                           CGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973083.5070544/1‑150 (MQ=60)
                                                                                                                           CGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG                          >  SRR19973083.3929503/1‑150 (MQ=60)
                                                                                                                           CGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCCCCATTCAGCGCG                          >  SRR19973083.3929497/1‑150 (MQ=60)
                                                                                                                            GTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGG                         >  SRR19973083.4575374/1‑150 (MQ=60)
                                                                                                                            GTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGG                         <  SRR19973083.1275595/150‑1 (MQ=60)
                                                                                                                             TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA                        >  SRR19973083.3639911/1‑150 (MQ=60)
                                                                                                                              TTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAT                       <  SRR19973083.3592135/150‑1 (MQ=60)
                                                                                                                               TTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATT                      <  SRR19973083.1156365/150‑1 (MQ=60)
                                                                                                                                TCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTC                     >  SRR19973083.1134450/1‑150 (MQ=60)
                                                                                                                                   TTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATA                  >  SRR19973083.626979/1‑150 (MQ=60)
                                                                                                                                    TCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT                 >  SRR19973083.2166691/1‑150 (MQ=60)
                                                                                                                                    TCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT                 <  SRR19973083.332049/150‑1 (MQ=60)
                                                                                                                                     CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA                >  SRR19973083.1867868/1‑150 (MQ=60)
                                                                                                                                         TGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTT            <  SRR19973083.4760534/150‑1 (MQ=60)
                                                                                                                                          GAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTT           >  SRR19973083.1698048/1‑150 (MQ=60)
                                                                                                                                          GAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTT           >  SRR19973083.1698256/1‑150 (MQ=60)
                                                                                                                                          GAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTT           >  SRR19973083.2306229/1‑150 (MQ=60)
                                                                                                                                          GAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTT           <  SRR19973083.390657/150‑1 (MQ=60)
                                                                                                                                          GAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTT           <  SRR19973083.3014179/150‑1 (MQ=60)
                                                                                                                                           AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          <  SRR19973083.4840908/150‑1 (MQ=60)
                                                                                                                                           AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          >  SRR19973083.1463154/1‑150 (MQ=60)
                                                                                                                                           AAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTG          <  SRR19973083.4840772/150‑1 (MQ=60)
                                                                                                                                             ACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAC        <  SRR19973083.669832/150‑1 (MQ=60)
                                                                                                                                             ACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAC        <  SRR19973083.669721/150‑1 (MQ=60)
                                                                                                                                               CAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCT      <  SRR19973083.1599433/150‑1 (MQ=60)
                                                                                                                                                AGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTT     >  SRR19973083.2899459/1‑150 (MQ=60)
                                                                                                                                                AGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTT     >  SRR19973083.2899677/1‑150 (MQ=60)
                                                                                                                                                   TAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  <  SRR19973083.869217/150‑1 (MQ=60)
                                                                                                                                                   TAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  SRR19973083.3091752/1‑150 (MQ=60)
                                                                                                                                                   GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  SRR19973083.2543641/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTT  >  CP009273/2450199‑2450495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑