Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,770 C→A 9.4% V623V (GTG→GTT)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,7700CA 9.4% 706.7 / 31.9 201V623V (GTG→GTT) fadJfused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (104/77);  new base A (7/12);  total (111/90)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 213 total aligned reads displayed.

CCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTG  >  CP009273/2450625‑2450917
                                                                                                                                                 |                                                                                                                                                   
ccGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATcac                                                                                                                                                   >  2:110252/1‑148 (MQ=255)
 cgcgCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATcaca                                                                                                                                                  <  2:120200/148‑1 (MQ=255)
        agagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATaacac                                                                                                                                                 <  1:625567/142‑1 (MQ=255)
        agagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATaacac                                                                                                                                                 >  2:625567/1‑142 (MQ=255)
        agagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGtt                                                                                                                                           <  1:639643/148‑1 (MQ=255)
         gagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTc                                                                                                                                          >  2:3240575/1‑148 (MQ=255)
         gagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTc                                                                                                                                          <  1:2209792/148‑1 (MQ=255)
              tCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCaa                                                                                                                                     >  1:280275/1‑148 (MQ=255)
               cGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAAc                                                                                                                                    <  2:1625872/148‑1 (MQ=255)
               cGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAAc                                                                                                                                    >  1:420519/1‑148 (MQ=255)
                       cGGAACTGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtg                                                                                                                            <  2:1823959/148‑1 (MQ=255)
                       cGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtg                                                                                                                            <  1:2913982/148‑1 (MQ=255)
                       cGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtg                                                                                                                            >  1:1181503/1‑148 (MQ=255)
                       cGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtg                                                                                                                            >  1:2574808/1‑148 (MQ=255)
                         gAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtgcg                                                                                                                          <  2:2088818/148‑1 (MQ=255)
                            cGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACCACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGgag                                                                                                                       <  2:961932/148‑1 (MQ=255)
                              gTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAt                                                                                                                     <  2:420519/148‑1 (MQ=255)
                                 cACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg                                                                                                                  <  1:2407222/148‑1 (MQ=255)
                                  aCCGAGAAAGCGCGGAAAACCACTGCCAACGACCGCGCCAACATCCCCGTCACGCACGCTACGGATAACCTGCTCATCACCACAACGTACTGCTTCATTCAGCATCCACATCACACCCCGTTCAGCAACCTGCGGTGCGGAGATTCGc                                                                                                                 <  2:2101433/148‑1 (MQ=255)
                                        aaaTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGc                                                                                                           >  1:999436/1‑148 (MQ=255)
                                         aaTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGcc                                                                                                          >  1:1926762/1‑148 (MQ=255)
                                          aTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGccc                                                                                                         >  1:2233185/1‑148 (MQ=255)
                                          aTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGccc                                                                                                         <  2:1723730/148‑1 (MQ=255)
                                             gCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtg                                                                                                      <  2:1926399/148‑1 (MQ=255)
                                             gCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtg                                                                                                      <  2:80277/148‑1 (MQ=255)
                                                gAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgt                                                                                                   >  1:705176/1‑148 (MQ=255)
                                                gAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgt                                                                                                   >  1:1574210/1‑148 (MQ=255)
                                                gAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgt                                                                                                   >  1:557520/1‑148 (MQ=255)
                                                 aaaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgtg                                                                                                  >  1:30365/1‑148 (MQ=255)
                                                  aaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGc                                                                                                 >  2:1071331/1‑148 (MQ=255)
                                                  aaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGc                                                                                                 >  2:2723388/1‑148 (MQ=255)
                                                  aaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGc                                                                                                 <  1:3103120/148‑1 (MQ=255)
                                                  aaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGc                                                                                                 <  2:261306/148‑1 (MQ=255)
                                                   aaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGcc                                                                                                >  2:3158418/1‑148 (MQ=255)
                                                    aCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCa                                                                                               >  1:1790365/1‑148 (MQ=255)
                                                     ccAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCaa                                                                                              >  1:1018608/1‑148 (MQ=255)
                                                     ccAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCaa                                                                                              <  1:1117962/148‑1 (MQ=255)
                                                       aaTGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgtg                                                                                                  <  1:1368116/142‑1 (MQ=255)
                                                        aTGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCa                                                                                           <  1:342214/148‑1 (MQ=255)
                                                          gCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGc                                                                                         >  2:1268940/1‑148 (MQ=255)
                                                            cAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCgg                                                                                       >  2:2656749/1‑148 (MQ=255)
                                                             aaaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCggg                                                                                      >  1:2175132/1‑148 (MQ=255)
                                                             aaaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCggg                                                                                      >  1:2174521/1‑148 (MQ=255)
                                                                 aCCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTaaa                                                                                  <  1:1289759/148‑1 (MQ=255)
                                                                   cgcgCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATg                                                                                >  1:32156/1‑148 (MQ=255)
                                                                     cgcCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGc                                                                              >  1:2005236/1‑148 (MQ=255)
                                                                        cAAGATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg                                                                           <  2:2453153/148‑1 (MQ=255)
                                                                         aaTATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGa                                                                          <  1:2990719/148‑1 (MQ=255)
                                                                          atatCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGAt                                                                         >  1:1024908/1‑148 (MQ=255)
                                                                             tCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGa                                                                      >  1:1618901/1‑148 (MQ=255)
                                                                              ccccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACCCACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAc                                                                     <  2:14148/148‑1 (MQ=255)
                                                                              ccccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAc                                                                     <  2:13327/148‑1 (MQ=255)
                                                                               cccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGa                                                                      <  1:1811165/146‑1 (MQ=255)
                                                                                   tCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACCCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGtt                                                                <  2:2835672/148‑1 (MQ=255)
                                                                                    cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttt                                                               >  1:2167794/1‑148 (MQ=255)
                                                                                    cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttt                                                               >  2:1713393/1‑148 (MQ=255)
                                                                                     acgcacgcTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGtttt                                                              >  2:1441949/1‑148 (MQ=255)
                                                                                        cacgcTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTg                                                           <  1:603994/148‑1 (MQ=255)
                                                                                            cTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCttt                                                       <  2:295177/148‑1 (MQ=255)
                                                                                            cTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCttt                                                       <  1:899297/148‑1 (MQ=255)
                                                                                             tACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCtttt                                                      <  2:2279532/148‑1 (MQ=255)
                                                                                                  aTAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc                                                 >  1:3042691/1‑148 (MQ=255)
                                                                                                  aTAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc                                                 <  1:670794/148‑1 (MQ=255)
                                                                                                  aTAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc                                                 >  1:1722868/1‑148 (MQ=255)
                                                                                                   tAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGccc                                                >  2:1258129/1‑148 (MQ=255)
                                                                                                      ccTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGTCCTGTTTTTTGCTTTTACGCCCttt                                             <  2:758741/148‑1 (MQ=255)
                                                                                                        tGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCt                                           >  1:1592665/1‑148 (MQ=255)
                                                                                                         gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                          >  2:3063438/1‑148 (MQ=255)
                                                                                                         gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                          >  1:909036/1‑148 (MQ=255)
                                                                                                         gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                          >  1:1188484/1‑148 (MQ=255)
                                                                                                         gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                          >  1:1613339/1‑148 (MQ=255)
                                                                                                             atcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCa                                      <  2:1894376/148‑1 (MQ=255)
                                                                                                             atcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCa                                      <  1:2635138/148‑1 (MQ=255)
                                                                                                              tcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCAt                                     >  2:1912678/1‑148 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 >  1:1075223/1‑148 (MQ=255)
                                                                                                                   acaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGa                                <  2:2781627/148‑1 (MQ=255)
                                                                                                                   acaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGa                                <  2:3013432/148‑1 (MQ=255)
                                                                                                                   acaACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGa                                <  1:75274/148‑1 (MQ=255)
                                                                                                                     aaCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATa                              >  1:1184331/1‑148 (MQ=255)
                                                                                                                      aCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAg                             <  2:904288/148‑1 (MQ=255)
                                                                                                                      aCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAg                             <  2:2189826/148‑1 (MQ=255)
                                                                                                                      aCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAg                             <  1:1441850/148‑1 (MQ=255)
                                                                                                                        gTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGaa                           <  2:572845/148‑1 (MQ=255)
                                                                                                                         tACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGaaa                          >  1:1057080/1‑148 (MQ=255)
                                                                                                                           cTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAAcc                        <  2:623439/148‑1 (MQ=255)
                                                                                                                           cTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAAcc                        <  2:888070/148‑1 (MQ=255)
                                                                                                                            tGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAAccc                       >  1:1962821/1‑148 (MQ=255)
                                                                                                                                 cattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGcc                  <  1:1394288/148‑1 (MQ=255)
                                                                                                                                  attcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCa                 <  2:452473/148‑1 (MQ=255)
                                                                                                                                    tcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCAtt               >  2:53142/1‑148 (MQ=255)
                                                                                                                                     caGCATCAACATCACACCCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTCCTGACCATAAAGATAGAAACCCCGGCCAttt              <  2:1901770/148‑1 (MQ=255)
                                                                                                                                          tCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCt         >  2:3198643/1‑148 (MQ=255)
                                                                                                                                          tCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCt         >  2:1057210/1‑148 (MQ=255)
                                                                                                                                           cAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTg        >  2:1998749/1‑148 (MQ=255)
                                                                                                                                            aaCATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGc       <  1:463573/148‑1 (MQ=255)
                                                                                                                                             aCATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGcc      >  2:439055/1‑148 (MQ=255)
                                                                                                                                              cATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCt     <  1:1713393/148‑1 (MQ=255)
                                                                                                                                               aTCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCtt    <  2:2280538/148‑1 (MQ=255)
                                                                                                                                                 cacacaCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTg  >  2:1569936/1‑148 (MQ=255)
                                                                                                                                                 cacacaCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTg  <  1:1894855/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTG  >  CP009273/2450625‑2450917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A