Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,452,454 G→T 69.0% P62Q (CCG→CAG)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,452,4540GT69.0% 303.3 / 246.5 232P62Q (CCG→CAG) fadJfused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (35/37);  new base T (84/76);  total (119/113)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

CGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452308‑2452602
                                                                                                                                                  |                                                                                                                                                    
cgcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGtt                                                                                                                                                   <  1:4901621/150‑1 (MQ=255)
cgcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGtt                                                                                                                                                   >  1:2518658/1‑150 (MQ=255)
 gcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGttt                                                                                                                                                  >  1:4721921/1‑150 (MQ=255)
 gcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGttt                                                                                                                                                  <  1:836423/150‑1 (MQ=255)
    aTGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGc                                                                                                                                               >  2:4362149/1‑150 (MQ=255)
     tGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCg                                                                                                                                              >  2:4546654/1‑150 (MQ=255)
     tGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCg                                                                                                                                              >  2:4755282/1‑150 (MQ=255)
      gAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGACGTTGTCCTGTTTAGCgg                                                                                                                                             >  2:1879999/1‑150 (MQ=255)
          aGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAc                                                                                                                                         <  2:4137225/150‑1 (MQ=255)
          aGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGAc                                                                                                                                         <  2:3843487/150‑1 (MQ=255)
           gCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACa                                                                                                                                        >  2:1133872/1‑150 (MQ=255)
           gCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACa                                                                                                                                        >  1:3673606/1‑150 (MQ=255)
              gcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAac                                                                                                                                     <  1:3755166/150‑1 (MQ=255)
                    aCCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACg                                                                                                                               >  2:1800150/1‑150 (MQ=255)
                        gAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTc                                                                                                                           <  2:5074699/150‑1 (MQ=255)
                          aTGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGc                                                                                                                         >  2:4035839/1‑150 (MQ=255)
                           tGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCa                                                                                                                        <  2:2057774/150‑1 (MQ=255)
                              gCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAAct                                                                                                                     >  2:427541/1‑150 (MQ=255)
                              gCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGTAGACAAACACCACGCCACGCAAct                                                                                                                     >  1:2596819/1‑150 (MQ=255)
                                  aGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                                 >  1:2736959/1‑150 (MQ=255)
                                  aGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                                 <  1:753881/150‑1 (MQ=255)
                                   gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTg                                                                                                                <  2:651254/150‑1 (MQ=255)
                                      tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGttt                                                                                                             >  2:4521415/1‑150 (MQ=255)
                                      tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGttt                                                                                                             >  2:3856225/1‑150 (MQ=255)
                                          tctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCAc                                                                                                         >  2:2178707/1‑150 (MQ=255)
                                           ctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACg                                                                                                        >  2:2441753/1‑150 (MQ=255)
                                           ctcCGCCATCAACTGTTGGCCCAGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACg                                                                                                        <  2:7005/150‑1 (MQ=255)
                                            tcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACgg                                                                                                       >  1:1533619/1‑150 (MQ=255)
                                               gCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGt                                                                                                    <  2:280976/150‑1 (MQ=255)
                                               gCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTGTGTTTTCACGGAGt                                                                                                    >  2:3647227/1‑150 (MQ=255)
                                                 cATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                                  >  2:416534/1‑150 (MQ=255)
                                                    cAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCtt                                                                                               <  1:4521335/150‑1 (MQ=255)
                                                    cAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCtt                                                                                               <  1:4181935/150‑1 (MQ=255)
                                                     aaCTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTta                                                                                              <  1:4320887/150‑1 (MQ=255)
                                                     aaCTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTta                                                                                              <  1:4361991/150‑1 (MQ=255)
                                                        tGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                           >  1:667515/1‑150 (MQ=255)
                                                        tGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                           <  2:3454509/150‑1 (MQ=255)
                                                          ttGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTaataat                                                                                         <  2:2605346/150‑1 (MQ=255)
                                                           tGGACCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGCAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATg                                                                                        >  2:1441250/1‑150 (MQ=255)
                                                            ggCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATgg                                                                                       <  1:442894/150‑1 (MQ=255)
                                                            ggCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATgg                                                                                       <  1:1604721/150‑1 (MQ=255)
                                                               ccTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgc                                                                                    >  2:1829953/1‑150 (MQ=255)
                                                               ccTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgc                                                                                    >  1:1336350/1‑150 (MQ=255)
                                                               ccTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgc                                                                                    >  1:940460/1‑150 (MQ=255)
                                                                cTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgcg                                                                                   <  2:3552610/150‑1 (MQ=255)
                                                                cTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgcg                                                                                   <  2:3552762/150‑1 (MQ=255)
                                                                     gcgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCt                                                                              <  1:2917992/150‑1 (MQ=255)
                                                                       gcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCAGGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCTCCTGc                                                                            <  2:1758930/150‑1 (MQ=255)
                                                                        ccAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCg                                                                           >  2:3385576/1‑150 (MQ=255)
                                                                         cAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCACCTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGa                                                                          >  2:4433113/1‑150 (MQ=255)
                                                                          agagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAg                                                                         <  2:1456105/150‑1 (MQ=255)
                                                                           gagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAgg                                                                        >  1:5151316/1‑150 (MQ=255)
                                                                            agCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGc                                                                       <  2:2912290/150‑1 (MQ=255)
                                                                             gCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCa                                                                      >  2:3854290/1‑150 (MQ=255)
                                                                              cTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCaa                                                                     >  2:2594427/1‑150 (MQ=255)
                                                                                tCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAc                                                                   <  1:3393069/150‑1 (MQ=255)
                                                                                   gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                                <  1:3620430/150‑1 (MQ=255)
                                                                                   gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                                <  1:711982/150‑1 (MQ=255)
                                                                                   gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                                <  2:4629214/150‑1 (MQ=255)
                                                                                   gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                                <  2:1344755/150‑1 (MQ=255)
                                                                                   gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                                >  2:22671/1‑150 (MQ=255)
                                                                                     ttcttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGc                                                              >  1:1750548/1‑150 (MQ=255)
                                                                                       cttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCt                                                            >  1:4076095/1‑150 (MQ=255)
                                                                                        ttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCtt                                                           >  2:3337379/1‑150 (MQ=255)
                                                                                        ttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCtt                                                           >  2:3158616/1‑150 (MQ=255)
                                                                                        ttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCtt                                                           <  2:282703/150‑1 (MQ=255)
                                                                                        ttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGAGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCtt                                                           <  1:1859144/150‑1 (MQ=255)
                                                                                         tGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTc                                                          <  1:1250075/150‑1 (MQ=255)
                                                                                          gcgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCa                                                         <  1:4289486/150‑1 (MQ=255)
                                                                                                 tttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTAtt                                                  >  1:3885410/1‑150 (MQ=255)
                                                                                                 tttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGAGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCGTCAGGGTAtt                                                  >  2:4257895/1‑150 (MQ=255)
                                                                                                     cAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtt                                              <  2:381035/150‑1 (MQ=255)
                                                                                                       gTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtttt                                            >  1:4659625/1‑150 (MQ=255)
                                                                                                       gTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtttt                                            >  1:3166012/1‑150 (MQ=255)
                                                                                                        ttGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtc                                           <  1:4755282/150‑1 (MQ=255)
                                                                                                        ttGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtc                                           <  1:4546642/150‑1 (MQ=255)
                                                                                                        ttGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtc                                           >  2:4051894/1‑150 (MQ=255)
                                                                                                         tGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtct                                          <  1:3592866/150‑1 (MQ=255)
                                                                                                          gCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtctc                                         <  1:37936/150‑1 (MQ=255)
                                                                                                          gCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGACAAACTCCGCCTTCAGGGTATTCATTTtctc                                         <  1:4852302/150‑1 (MQ=255)
                                                                                                            cGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAc                                       <  1:5095836/150‑1 (MQ=255)
                                                                                                             gATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAcc                                      >  2:2736910/1‑150 (MQ=255)
                                                                                                               tCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCg                                    >  1:3773588/1‑150 (MQ=255)
                                                                                                                  tGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTa                                 <  1:1881951/150‑1 (MQ=255)
                                                                                                                     tGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACg                               >  1:2496802/1‑149 (MQ=255)
                                                                                                                       aTGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATACTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCg                            <  1:1238164/150‑1 (MQ=255)
                                                                                                                        tGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGa                           >  1:2970631/1‑150 (MQ=255)
                                                                                                                          tCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATg                         <  2:622058/150‑1 (MQ=255)
                                                                                                                                   gCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACg                >  2:4385341/1‑150 (MQ=255)
                                                                                                                                    cAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACgg               >  1:2192033/1‑150 (MQ=255)
                                                                                                                                    cAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACgg               >  1:2117845/1‑150 (MQ=255)
                                                                                                                                     aaTGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTCTTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGc              >  1:1975227/1‑150 (MQ=255)
                                                                                                                                     aaTGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGc              >  2:4932980/1‑150 (MQ=255)
                                                                                                                                       tGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCACACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCaa            >  2:2193523/1‑150 (MQ=255)
                                                                                                                                          aGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgt         >  2:2526563/1‑150 (MQ=255)
                                                                                                                                          aGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgt         <  1:3503078/150‑1 (MQ=255)
                                                                                                                                           gTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgtt        >  1:2413492/1‑150 (MQ=255)
                                                                                                                                               tCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTcc    >  1:1133096/1‑150 (MQ=255)
                                                                                                                                                ccGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCa   <  2:1993569/150‑1 (MQ=255)
                                                                                                                                                ccGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCa   <  2:1230375/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452308‑2452602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452305‑2452602
                                                                                                                                                     |                                                                                                                                                    
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      <  SRR19973083.1587425/150‑1 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      <  SRR19973083.1587418/150‑1 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      >  SRR19973083.1362483/1‑150 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      >  SRR19973083.1362512/1‑150 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      >  SRR19973083.1362534/1‑150 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      >  SRR19973083.3774062/1‑150 (MQ=60)
   CGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTT                                                                                                                                                   <  SRR19973083.4922689/150‑1 (MQ=60)
   CGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTT                                                                                                                                                   >  SRR19973083.2531460/1‑150 (MQ=60)
    GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTT                                                                                                                                                  >  SRR19973083.4742112/1‑150 (MQ=60)
    GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTT                                                                                                                                                  <  SRR19973083.840381/150‑1 (MQ=60)
     CCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGCTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTA                                                                                                                                                 <  SRR19973083.4497821/150‑1 (MQ=60)
       ATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGATTTGCAGTTGCCGAACAAGTTGATGTCTTAGCCAGCAAAGAAGATGTCCGGTTTATC                                                                                                                                               >  SRR19973083.2745510/1‑150 (MQ=60)
              GCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACA                                                                                                                                        >  SRR19973083.3690382/1‑150 (MQ=60)
              GCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACA                                                                                                                                        <  SRR19973083.788778/150‑1 (MQ=60)
                 GCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAAC                                                                                                                                     <  SRR19973083.4904159/150‑1 (MQ=60)
                 GCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAAC                                                                                                                                     <  SRR19973083.3772179/150‑1 (MQ=60)
                 GCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAAC                                                                                                                                     <  SRR19973083.3772290/150‑1 (MQ=60)
                     TAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCA                                                                                                                                 >  SRR19973083.1880641/1‑150 (MQ=60)
                       ACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACG                                                                                                                               <  SRR19973083.1895107/150‑1 (MQ=60)
                           GAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTC                                                                                                                           <  SRR19973083.825644/150‑1 (MQ=60)
                            AATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGACAGAGCTTCCGCGTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCACAGAAGTTGTCCGGTTTATCGGAGACAAACACCACGCCACG                                                                                                                          >  SRR19973083.3236313/1‑150 (MQ=60)
                                 GCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGTAGACAAACACCACGCCACGCAACT                                                                                                                     >  SRR19973083.2609893/1‑150 (MQ=60)
                                 GCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACT                                                                                                                     >  SRR19973083.2609716/1‑150 (MQ=60)
                                   AAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCT                                                                                                                   <  SRR19973083.3805108/150‑1 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGTGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 <  SRR19973083.4864846/150‑1 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAGGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 >  SRR19973083.4388165/1‑150 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 >  SRR19973083.2750616/1‑150 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 <  SRR19973083.757573/150‑1 (MQ=60)
                                      GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                                >  SRR19973083.1174574/1‑150 (MQ=60)
                                        ATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTT                                                                                                              >  SRR19973083.1465560/1‑150 (MQ=60)
                                              CTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACG                                                                                                        <  SRR19973083.1475749/150‑1 (MQ=60)
                                               TCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGG                                                                                                       >  SRR19973083.2199326/1‑150 (MQ=60)
                                               TCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGG                                                                                                       >  SRR19973083.1541338/1‑150 (MQ=60)
                                                CCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGA                                                                                                      <  SRR19973083.5122428/150‑1 (MQ=60)
                                                CCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGA                                                                                                      >  SRR19973083.2954418/1‑150 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973083.4540947/150‑1 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               >  SRR19973083.3766357/1‑150 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973083.4200350/150‑1 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973083.4541027/150‑1 (MQ=60)
                                                        AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                              <  SRR19973083.4339576/150‑1 (MQ=60)
                                                        AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                              <  SRR19973083.4339645/150‑1 (MQ=60)
                                                        AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                              <  SRR19973083.4339727/150‑1 (MQ=60)
                                                        AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                              <  SRR19973083.4380919/150‑1 (MQ=60)
                                                         ACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTGTGTTTTCACGGAGTTGCTTAA                                                                                             >  SRR19973083.4287109/1‑150 (MQ=60)
                                                           TGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATA                                                                                           >  SRR19973083.5092308/1‑150 (MQ=60)
                                                           TGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATA                                                                                           >  SRR19973083.670863/1‑150 (MQ=60)
                                                            GTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAA                                                                                          >  SRR19973083.4728088/1‑150 (MQ=60)
                                                            GTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAA                                                                                          >  SRR19973083.4727975/1‑150 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973083.1612754/150‑1 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973083.444870/150‑1 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973083.444890/150‑1 (MQ=60)
                                                                  CCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGC                                                                                    >  SRR19973083.1342873/1‑150 (MQ=60)
                                                                  CCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGC                                                                                    >  SRR19973083.945052/1‑150 (MQ=60)
                                                                   CTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCG                                                                                   <  SRR19973083.273913/150‑1 (MQ=60)
                                                                     GCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCA                                                                                 <  SRR19973083.3897381/150‑1 (MQ=60)
                                                                        GCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCT                                                                              <  SRR19973083.3500112/150‑1 (MQ=60)
                                                                        GCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCT                                                                              <  SRR19973083.2932268/150‑1 (MQ=60)
                                                                        GCGCCAGAGCTTCCGCTTCTTGCGCCGTTGTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCT                                                                              <  SRR19973083.2521586/150‑1 (MQ=60)
                                                                          GCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCATTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGATGCTTAATAATGGCGCGCACCTGC                                                                            >  SRR19973083.1784807/1‑150 (MQ=60)
                                                                              GAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGG                                                                        >  SRR19973083.5173192/1‑150 (MQ=60)
                                                                              GAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGG                                                                        <  SRR19973083.886417/150‑1 (MQ=60)
                                                                                   TCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAC                                                                   >  SRR19973083.4745671/1‑150 (MQ=60)
                                                                                   TCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAC                                                                   <  SRR19973083.3408773/150‑1 (MQ=60)
                                                                                      GCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCC                                                                <  SRR19973083.3636906/150‑1 (MQ=60)
                                                                                      GCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCC                                                                <  SRR19973083.715520/150‑1 (MQ=60)
                                                                                      GCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCC                                                                <  SRR19973083.3637198/150‑1 (MQ=60)
                                                                                      GCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCC                                                                <  SRR19973083.3637001/150‑1 (MQ=60)
                                                                                       CTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCG                                                               >  SRR19973083.412556/1‑150 (MQ=60)
                                                                                        TTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGC                                                              >  SRR19973083.1759131/1‑150 (MQ=60)
                                                                                        TTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGC                                                              >  SRR19973083.2012675/1‑150 (MQ=60)
                                                                                          CTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCT                                                            >  SRR19973083.4087175/1‑150 (MQ=60)
                                                                                          CTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCT                                                            >  SRR19973083.4094175/1‑150 (MQ=60)
                                                                                           TTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTT                                                           <  SRR19973083.4889214/150‑1 (MQ=60)
                                                                                           TTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTT                                                           <  SRR19973083.3753646/150‑1 (MQ=60)
                                                                                           TTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGAGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTT                                                           <  SRR19973083.1868535/150‑1 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          >  SRR19973083.4332226/1‑150 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          <  SRR19973083.1256178/150‑1 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          >  SRR19973083.1727431/1‑150 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          >  SRR19973083.1265291/1‑150 (MQ=60)
                                                                                             GCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCA                                                         <  SRR19973083.4308164/150‑1 (MQ=60)
                                                                                                CCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTATCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAACTGCGAGGCAAACTCCGCCTTCAGGG                                                      >  SRR19973083.3594119/1‑150 (MQ=60)
                                                                                                  GTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTA                                                    <  SRR19973083.2438558/150‑1 (MQ=60)
                                                                                                    TTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATT                                                  >  SRR19973083.3902746/1‑150 (MQ=60)
                                                                                                          GTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTT                                            >  SRR19973083.3181105/1‑150 (MQ=60)
                                                                                                          GTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTT                                            >  SRR19973083.4679680/1‑150 (MQ=60)
                                                                                                          GTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGATATTCATTTT                                            >  SRR19973083.561744/1‑150 (MQ=60)
                                                                                                           TTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTC                                           <  SRR19973083.4775563/150‑1 (MQ=60)
                                                                                                           TTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTC                                           <  SRR19973083.4566361/150‑1 (MQ=60)
                                                                                                           TTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTC                                           <  SRR19973083.4566349/150‑1 (MQ=60)
                                                                                                            TGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCT                                          <  SRR19973083.3609324/150‑1 (MQ=60)
                                                                                                            TGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCT                                          <  SRR19973083.745425/150‑1 (MQ=60)
                                                                                                             GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                         <  SRR19973083.38234/150‑1 (MQ=60)
                                                                                                             GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGACAAACTCCGCCTTCAGGGTATTCATTTTCTC                                         <  SRR19973083.4873134/150‑1 (MQ=60)
                                                                                                               CGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAC                                       <  SRR19973083.1560483/150‑1 (MQ=60)
                                                                                                               CGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAC                                       <  SRR19973083.5117591/150‑1 (MQ=60)
                                                                                                                  TCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCG                                    >  SRR19973083.3790636/1‑150 (MQ=60)
                                                                                                                   CATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGG                                   <  SRR19973083.674246/150‑1 (MQ=60)
                                                                                                                     TGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTA                                 <  SRR19973083.1891490/150‑1 (MQ=60)
                                                                                                                      GTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAC                                <  SRR19973083.2753369/150‑1 (MQ=60)
                                                                                                                        TGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGA                              >  SRR19973083.2509534/1‑150 (MQ=60)
                                                                                                                         GATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTC                             <  SRR19973083.2454274/150‑1 (MQ=60)
                                                                                                                         GATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTC                             <  SRR19973083.3101268/150‑1 (MQ=60)
                                                                                                                          ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATACTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                            <  SRR19973083.1244222/150‑1 (MQ=60)
                                                                                                                          ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                            <  SRR19973083.1244258/150‑1 (MQ=60)
                                                                                                                           TGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGA                           >  SRR19973083.2985060/1‑150 (MQ=60)
                                                                                                                           TGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGA                           >  SRR19973083.360859/1‑150 (MQ=60)
                                                                                                                           TGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTGCAGGGTAGTCATTTTCTCACCCGGTCCGTCGA                           >  SRR19973083.2988898/1‑150 (MQ=60)
                                                                                                                                GCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTCTTCATTTTCTCACCCGGTACGTCGATGGTG                      >  SRR19973083.393822/1‑150 (MQ=60)
                                                                                                                                    CAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGAAGGGGATAA                  >  SRR19973083.2403390/1‑150 (MQ=60)
                                                                                                                                       CAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGG               >  SRR19973083.2128538/1‑150 (MQ=60)
                                                                                                                                       CAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGG               >  SRR19973083.2203022/1‑150 (MQ=60)
                                                                                                                                        AATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTCTTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGC              >  SRR19973083.1985320/1‑150 (MQ=60)
                                                                                                                                             AGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGT         <  SRR19973083.3519135/150‑1 (MQ=60)
                                                                                                                                             AGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGT         >  SRR19973083.1768009/1‑150 (MQ=60)
                                                                                                                                              GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT        >  SRR19973083.2425890/1‑150 (MQ=60)
                                                                                                                                                 GTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTC     >  SRR19973083.551016/1‑150 (MQ=60)
                                                                                                                                                  TCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCC    >  SRR19973083.1138772/1‑150 (MQ=60)
                                                                                                                                                   CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA   >  SRR19973083.3175602/1‑150 (MQ=60)
                                                                                                                                                    CGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  SRR19973083.2125084/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452305‑2452602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑