Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,452,454 G→T 100% P62Q (CCG→CAG)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,452,4540GT99.0% 638.0 / ‑3.4 195P62Q (CCG→CAG) fadJfused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (67/125);  minor base C (2/0);  total (70/125)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  CP009273/2452309‑2452598
                                                                                                                                                 |                                                                                                                                                
gcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGt                                                                                                                                                <  2:1348065/148‑1 (MQ=255)
gcCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGt                                                                                                                                                <  2:1593032/148‑1 (MQ=255)
 ccATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGtt                                                                                                                                               <  1:2170614/148‑1 (MQ=255)
  cATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGttt                                                                                                                                              <  2:781273/148‑1 (MQ=255)
   attaaTAGACGCGATCACAAGTATGGGCTAAGCTTGAATCTCCGCCATCATCTGTTGGCCATGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGATTTGCAGTTGCAGATCTTGTTGATTTCTGCGCCAGCAATGATGTTGTCCTGTTTa                                                                                                                                             <  2:2756537/145‑1 (MQ=255)
   aTGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTa                                                                                                                                             <  1:2000317/148‑1 (MQ=255)
   aTGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTa                                                                                                                                             <  2:2709758/148‑1 (MQ=255)
   aTGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTa                                                                                                                                             <  1:1874825/148‑1 (MQ=255)
     gAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGc                                                                                                                                           >  2:3173631/1‑148 (MQ=255)
       aTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCgg                                                                                                                                         <  2:1901287/148‑1 (MQ=255)
       aTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCgg                                                                                                                                         <  1:2190556/148‑1 (MQ=255)
           ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAc                                                                                                                                     >  2:1865410/1‑148 (MQ=255)
           ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAc                                                                                                                                     >  2:89639/1‑148 (MQ=255)
           ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCCGTTTAGCGGAGAc                                                                                                                                     >  2:1936097/1‑148 (MQ=255)
             gcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACaa                                                                                                                                   <  1:3137401/148‑1 (MQ=255)
             gcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACaa                                                                                                                                   <  1:3098966/148‑1 (MQ=255)
              cgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACaaa                                                                                                                                  >  2:2693613/1‑148 (MQ=255)
                     cTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACg                                                                                                                           >  1:1331265/1‑148 (MQ=255)
                      tGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGc                                                                                                                          <  2:602309/148‑1 (MQ=255)
                       gAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGcc                                                                                                                         >  1:3469263/1‑148 (MQ=255)
                          tGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCg                                                                                                                      <  2:909995/148‑1 (MQ=255)
                          tGGGCAAAGCATGAAACTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTATGCAGTTGCCGATCAAGTTGATGTCTGCTCCAGCAATTAAGTTGTCATGTTTAGCGGAGACAAACAACACTCCACg                                                                                                                      >  1:1386902/1‑148 (MQ=255)
                            ggCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCa                                                                                                                    <  2:2727672/148‑1 (MQ=255)
                              cAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTGTAGCGGAGACACACACCACGCCTCGCAAc                                                                                                                  >  2:2573122/1‑148 (MQ=255)
                                 aGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAActct                                                                                                               >  2:3017525/1‑148 (MQ=255)
                                 aGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAActct                                                                                                               <  1:298508/148‑1 (MQ=255)
                                  gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCtt                                                                                                              <  1:3356183/148‑1 (MQ=255)
                                  gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCATCGCAACTCtt                                                                                                              <  1:2155391/148‑1 (MQ=255)
                                   cATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                             >  1:1033446/1‑148 (MQ=255)
                                    aTGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTg                                                                                                            <  1:2429236/148‑1 (MQ=255)
                                     tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGt                                                                                                           <  1:527061/148‑1 (MQ=255)
                                     tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGt                                                                                                           <  1:1592657/148‑1 (MQ=255)
                                         tctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTc                                                                                                       <  1:2082675/148‑1 (MQ=255)
                                         tctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTc                                                                                                       <  1:2727146/148‑1 (MQ=255)
                                         tctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTc                                                                                                       <  2:707147/148‑1 (MQ=255)
                                         tctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTc                                                                                                       <  1:3005868/148‑1 (MQ=255)
                                                cATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGt                                                                                                >  2:196072/1‑148 (MQ=255)
                                                 aTCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGtt                                                                                               >  2:2497393/1‑148 (MQ=255)
                                                  tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              <  1:3473921/148‑1 (MQ=255)
                                                  tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              <  1:254430/148‑1 (MQ=255)
                                                   cAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGc                                                                                             <  1:1935056/148‑1 (MQ=255)
                                                    aaCTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCt                                                                                            >  2:1329523/1‑148 (MQ=255)
                                                       tGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaa                                                                                         <  1:1945417/148‑1 (MQ=255)
                                                         ttGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                       >  2:468540/1‑148 (MQ=255)
                                                          tGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaataa                                                                                      >  2:2920851/1‑148 (MQ=255)
                                                          tGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaataa                                                                                      >  1:933429/1‑148 (MQ=255)
                                                           ggCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTaataat                                                                                     <  1:1711731/148‑1 (MQ=255)
                                                           ggCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTaataat                                                                                     <  1:1711684/148‑1 (MQ=255)
                                                            gCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATg                                                                                    >  2:627228/1‑148 (MQ=255)
                                                            gCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCATCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATg                                                                                    <  1:3216281/148‑1 (MQ=255)
                                                                    gcgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAc                                                                            <  1:1257158/148‑1 (MQ=255)
                                                                     cgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAcc                                                                           <  2:814043/148‑1 (MQ=255)
                                                                       ccAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTg                                                                         <  2:3427848/148‑1 (MQ=255)
                                                                        cAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGc                                                                        >  2:2954844/1‑148 (MQ=255)
                                                                         agagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCg                                                                       <  1:1063423/148‑1 (MQ=255)
                                                                         agagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCg                                                                       <  2:1636790/148‑1 (MQ=255)
                                                                         agagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCg                                                                       <  2:1538781/148‑1 (MQ=255)
                                                                         agagCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCg                                                                       <  2:1538724/148‑1 (MQ=255)
                                                                            gCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAgg                                                                    <  1:2573692/148‑1 (MQ=255)
                                                                                  gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACt                                                              >  1:3271201/1‑148 (MQ=255)
                                                                                  gCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACt                                                              >  1:862439/1‑148 (MQ=255)
                                                                                   cttcttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTc                                                             <  1:1753165/148‑1 (MQ=255)
                                                                                        tGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCt                                                        <  1:2903982/148‑1 (MQ=255)
                                                                                        tGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCt                                                        <  1:2902971/148‑1 (MQ=255)
                                                                                         gcgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCtt                                                       <  2:2187426/148‑1 (MQ=255)
                                                                                          cgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCCAACTCCGCCTTc                                                      >  1:2230409/1‑148 (MQ=255)
                                                                                          cgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTc                                                      <  2:1762826/148‑1 (MQ=255)
                                                                                              gTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAggg                                                  <  2:2160658/148‑1 (MQ=255)
                                                                                               ttttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGt                                                 >  2:1411022/1‑148 (MQ=255)
                                                                                               ttttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGt                                                 >  2:1210542/1‑148 (MQ=255)
                                                                                                tttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTa                                                <  1:3312459/148‑1 (MQ=255)
                                                                                                  tGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTAtt                                              <  1:178593/148‑1 (MQ=255)
                                                                                                    cAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCa                                            <  2:3064153/148‑1 (MQ=255)
                                                                                                      gTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtt                                          <  2:1153716/148‑1 (MQ=255)
                                                                                                      gTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGACCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAtt                                          >  2:572357/1‑148 (MQ=255)
                                                                                                       ttGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACACACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAttt                                         <  2:1483949/148‑1 (MQ=255)
                                                                                                         gCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtc                                       <  1:2800441/148‑1 (MQ=255)
                                                                                                          ccGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtct                                      <  2:316631/148‑1 (MQ=255)
                                                                                                               cATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAccc                                 >  2:3176828/1‑148 (MQ=255)
                                                                                                                 tGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGTGTATTCATTTTCTCACCCgg                               <  2:933429/148‑1 (MQ=255)
                                                                                                                 tGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCgg                               >  2:122508/1‑148 (MQ=255)
                                                                                                                     gATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACg                           <  2:395615/148‑1 (MQ=255)
                                                                                                                      aTGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGt                          <  1:370950/148‑1 (MQ=255)
                                                                                                                      aTGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGt                          <  1:2937838/148‑1 (MQ=255)
                                                                                                                       tGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTc                         >  2:2548348/1‑148 (MQ=255)
                                                                                                                           tGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATg                     <  1:89667/148‑1 (MQ=255)
                                                                                                                           tGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATg                     <  1:1865410/148‑1 (MQ=255)
                                                                                                                            gcgcCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATgg                    <  1:2808221/148‑1 (MQ=255)
                                                                                                                              gcCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTg                  >  1:1946829/1‑148 (MQ=255)
                                                                                                                               ccAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGa                 <  2:670050/148‑1 (MQ=255)
                                                                                                                                 aGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATa               >  2:3172536/1‑148 (MQ=255)
                                                                                                                                 aGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATa               <  2:1127389/148‑1 (MQ=255)
                                                                                                                                   cAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAAc             >  2:139884/1‑148 (MQ=255)
                                                                                                                                       gAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGTTTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTTATAACGGCa         <  1:79/148‑1 (MQ=255)
                                                                                                                                        aaGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCaa        <  1:3173602/148‑1 (MQ=255)
                                                                                                                                        aaGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCaa        <  2:204802/148‑1 (MQ=255)
                                                                                                                                          gTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtg      >  2:2354664/1‑148 (MQ=255)
                                                                                                                                           ttGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgt     >  2:327068/1‑148 (MQ=255)
                                                                                                                                           ttGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgt     >  1:174079/1‑148 (MQ=255)
                                                                                                                                             gTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgttg   <  1:1434248/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  CP009273/2452309‑2452598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452305‑2452602
                                                                                                                                                     |                                                                                                                                                    
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTGGGGTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      <  SRR19973065.2354946/150‑1 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      <  SRR19973065.579892/150‑1 (MQ=60)
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCT                                                                                                                                                      <  SRR19973065.2354708/150‑1 (MQ=60)
 AGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTG                                                                                                                                                     <  SRR19973065.921510/150‑1 (MQ=60)
  GCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGT                                                                                                                                                    <  SRR19973065.934970/150‑1 (MQ=60)
     CCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCATCTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTA                                                                                                                                                 <  SRR19973065.2184518/150‑1 (MQ=60)
     CCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTA                                                                                                                                                 <  SRR19973065.2184546/150‑1 (MQ=60)
       ATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGC                                                                                                                                               <  SRR19973065.1887116/150‑1 (MQ=60)
       ATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGC                                                                                                                                               <  SRR19973065.1887169/150‑1 (MQ=60)
       ATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGC                                                                                                                                               <  SRR19973065.2013267/150‑1 (MQ=60)
         GAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGG                                                                                                                                             >  SRR19973065.1429648/1‑150 (MQ=60)
           ATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAG                                                                                                                                           <  SRR19973065.2204603/150‑1 (MQ=60)
           ATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAG                                                                                                                                           <  SRR19973065.3521507/150‑1 (MQ=60)
            TAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGA                                                                                                                                          <  SRR19973065.2168164/150‑1 (MQ=60)
            TAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGA                                                                                                                                          >  SRR19973065.3278142/1‑150 (MQ=60)
                 GCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAAC                                                                                                                                     <  SRR19973065.3156480/150‑1 (MQ=60)
                 GCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAAC                                                                                                                                     <  SRR19973065.3117884/150‑1 (MQ=60)
                      AACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCAC                                                                                                                                <  SRR19973065.2494135/150‑1 (MQ=60)
                       ACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACG                                                                                                                               >  SRR19973065.1340071/1‑150 (MQ=60)
                       ACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACG                                                                                                                               >  SRR19973065.2606508/1‑150 (MQ=60)
                         CTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCC                                                                                                                             >  SRR19973065.3490384/1‑150 (MQ=60)
                            AATGGGCAAAGCATGAAACTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTATGCAGTTGCCGATCAAGTTGATGTCTGCTCCAGCAATTAAGTTGTCATGTTTAGCGGAGACAAACAacactccacg                                                                                                                          >  SRR19973065.1396014/1‑140 (MQ=60)
                                    AAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTT                                                                                                                  >  SRR19973065.3144825/1‑150 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 <  SRR19973065.300437/150‑1 (MQ=60)
                                     AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                 >  SRR19973065.1040288/1‑150 (MQ=60)
                                      GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                                <  SRR19973065.3295937/150‑1 (MQ=60)
                                      GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                                <  SRR19973065.3376701/150‑1 (MQ=60)
                                      GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCATCGCAACTCTTTG                                                                                                                <  SRR19973065.2169254/150‑1 (MQ=60)
                                        ATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTT                                                                                                              <  SRR19973065.2573272/150‑1 (MQ=60)
                                        ATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTT                                                                                                              <  SRR19973065.2444568/150‑1 (MQ=60)
                                        ATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTT                                                                                                              >  SRR19973065.3334608/1‑150 (MQ=60)
                                         TGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTT                                                                                                             <  SRR19973065.1603296/150‑1 (MQ=60)
                                         TGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTT                                                                                                             <  SRR19973065.530580/150‑1 (MQ=60)
                                         TGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTT                                                                                                             <  SRR19973065.530520/150‑1 (MQ=60)
                                             TCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCAC                                                                                                         <  SRR19973065.3023604/150‑1 (MQ=60)
                                             TCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCAC                                                                                                         <  SRR19973065.2096023/150‑1 (MQ=60)
                                             TCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCAC                                                                                                         <  SRR19973065.2744110/150‑1 (MQ=60)
                                             TCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCAC                                                                                                         <  SRR19973065.3024280/150‑1 (MQ=60)
                                                 CGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAG                                                                                                     >  SRR19973065.1355287/1‑150 (MQ=60)
                                                      TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                                <  SRR19973065.3495057/150‑1 (MQ=60)
                                                      TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                                <  SRR19973065.3495055/150‑1 (MQ=60)
                                                      TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                                <  SRR19973065.256207/150‑1 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973065.1948200/150‑1 (MQ=60)
                                                       CAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973065.1947722/150‑1 (MQ=60)
                                                       CAACTGTGGGCCCGGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTT                                                                                               <  SRR19973065.1947684/150‑1 (MQ=60)
                                                         ACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAA                                                                                             >  SRR19973065.3185374/1‑150 (MQ=60)
                                                          CTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAAT                                                                                            >  SRR19973065.1550631/1‑150 (MQ=60)
                                                           TGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATA                                                                                           <  SRR19973065.1958775/150‑1 (MQ=60)
                                                           TGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATA                                                                                           <  SRR19973065.1958135/150‑1 (MQ=60)
                                                            GTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCACGCAACTCTTTGTTTTAACGGAGTTGGTTAATAA                                                                                          >  SRR19973065.939591/1‑150 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973065.1723121/150‑1 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973065.1723074/150‑1 (MQ=60)
                                                               GGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGG                                                                                       <  SRR19973065.1723085/150‑1 (MQ=60)
                                                                GCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCATCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGC                                                                                      <  SRR19973065.3235623/150‑1 (MQ=60)
                                                                        GCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCT                                                                              <  SRR19973065.1265438/150‑1 (MQ=60)
                                                                             AGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAG                                                                         <  SRR19973065.1070473/150‑1 (MQ=60)
                                                                              GAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGG                                                                        <  SRR19973065.395629/150‑1 (MQ=60)
                                                                                GCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCA                                                                      <  SRR19973065.2589751/150‑1 (MQ=60)
                                                                                    CCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCCAACT                                                                  >  SRR19973065.2898026/1‑150 (MQ=60)
                                                                                    CCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACT                                                                  >  SRR19973065.3291050/1‑150 (MQ=60)
                                                                                    CCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACT                                                                  >  SRR19973065.867991/1‑150 (MQ=60)
                                                                                       CTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCG                                                               <  SRR19973065.1764703/150‑1 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCCAACTCCGCCTTC                                                          >  SRR19973065.2244606/1‑150 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          <  SRR19973065.2921688/150‑1 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          <  SRR19973065.2920692/150‑1 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          >  SRR19973065.2244274/1‑150 (MQ=60)
                                                                                            TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                          <  SRR19973065.2920674/150‑1 (MQ=60)
                                                                                                    TTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATT                                                  <  SRR19973065.3332649/150‑1 (MQ=60)
                                                                                                      TGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCA                                                <  SRR19973065.906057/150‑1 (MQ=60)
                                                                                                      TGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCA                                                <  SRR19973065.180002/150‑1 (MQ=60)
                                                                                                           TTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTC                                           >  SRR19973065.281010/1‑150 (MQ=60)
                                                                                                           TTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTC                                           <  SRR19973065.2041702/150‑1 (MQ=60)
                                                                                                             GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                         <  SRR19973065.2817785/150‑1 (MQ=60)
                                                                                                             GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                         <  SRR19973065.2818847/150‑1 (MQ=60)
                                                                                                              CCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCA                                        >  SRR19973065.1141637/1‑150 (MQ=60)
                                                                                                              CCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCA                                        <  SRR19973065.1730999/150‑1 (MQ=60)
                                                                                                                      GTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAC                                <  SRR19973065.3032722/150‑1 (MQ=60)
                                                                                                                      GTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAC                                <  SRR19973065.1275824/150‑1 (MQ=60)
                                                                                                                         GATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTC                             <  SRR19973065.1680763/150‑1 (MQ=60)
                                                                                                                          ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                            <  SRR19973065.2955656/150‑1 (MQ=60)
                                                                                                                          ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                            <  SRR19973065.373547/150‑1 (MQ=60)
                                                                                                                            GTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGAT                          <  SRR19973065.1829273/150‑1 (MQ=60)
                                                                                                                            GTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGAT                          <  SRR19973065.298927/150‑1 (MQ=60)
                                                                                                                               TGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGT                       <  SRR19973065.90524/150‑1 (MQ=60)
                                                                                                                               TGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGT                       <  SRR19973065.90496/150‑1 (MQ=60)
                                                                                                                               TGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGT                       <  SRR19973065.2347166/150‑1 (MQ=60)
                                                                                                                               TGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGT                       <  SRR19973065.1877687/150‑1 (MQ=60)
                                                                                                                                GCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG                      >  SRR19973065.1959552/1‑150 (MQ=60)
                                                                                                                                GCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG                      <  SRR19973065.2825593/150‑1 (MQ=60)
                                                                                                                                     AGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAAC                 <  SRR19973065.2938602/150‑1 (MQ=60)
                                                                                                                                     AGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAAC                 <  SRR19973065.831559/150‑1 (MQ=60)
                                                                                                                                           GAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGTTTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTTATAACGGCAAT           <  SRR19973065.80/150‑1 (MQ=60)
                                                                                                                                            AAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATG          <  SRR19973065.3192797/150‑1 (MQ=60)
                                                                                                                                            AAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATG          <  SRR19973065.3192826/150‑1 (MQ=60)
                                                                                                                                             AGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGT         >  SRR19973065.175466/1‑150 (MQ=60)
                                                                                                                                             AGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGT         >  SRR19973065.176681/1‑150 (MQ=60)
                                                                                                                                                 GTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTC     <  SRR19973065.1443565/150‑1 (MQ=60)
                                                                                                                                                 GTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTC     <  SRR19973065.198865/150‑1 (MQ=60)
                                                                                                                                                  TCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACNTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCC    <  SRR19973065.1793355/150‑1 (MQ=60)
                                                                                                                                                   CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA   <  SRR19973065.2710337/150‑1 (MQ=60)
                                                                                                                                                    CTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  SRR19973065.2997862/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG  >  CP009273/2452305‑2452602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑