Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,452,454 G→T 100% P62Q (CCG→CAG)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,452,4540GT100.0% 691.0 / NA 206P62Q (CCG→CAG) fadJfused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (91/115);  total (91/115)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

CCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  CP009273/2452310‑2452598
                                                                                                                                                |                                                                                                                                                
ccATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGACCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGtt                                                                                                                                               <  1:2488356/148‑1 (MQ=255)
 cATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGttt                                                                                                                                              <  2:653807/148‑1 (MQ=255)
     aaTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCg                                                                                                                                          >  1:3729968/1‑148 (MQ=255)
       tAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGga                                                                                                                                        >  2:158163/1‑148 (MQ=255)
        aGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGgag                                                                                                                                       <  1:850483/148‑1 (MQ=255)
          ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAc                                                                                                                                     >  1:1736471/1‑148 (MQ=255)
          ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAc                                                                                                                                     >  1:1737527/1‑148 (MQ=255)
             cgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACaaa                                                                                                                                  <  1:2825588/148‑1 (MQ=255)
                 aaCCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAAcacc                                                                                                                              <  2:1291230/148‑1 (MQ=255)
                     tGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGc                                                                                                                          <  2:1979899/148‑1 (MQ=255)
                      gAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGcc                                                                                                                         >  1:3517083/1‑148 (MQ=255)
                           ggCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCa                                                                                                                    >  2:3018339/1‑148 (MQ=255)
                              aaaGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAAct                                                                                                                 <  1:447370/148‑1 (MQ=255)
                               aaGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAActc                                                                                                                <  2:1072630/148‑1 (MQ=255)
                               aaGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAActc                                                                                                                >  1:1508120/1‑148 (MQ=255)
                                 gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCtt                                                                                                              >  1:1786825/1‑148 (MQ=255)
                                 gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCtt                                                                                                              <  1:2335214/148‑1 (MQ=255)
                                 gCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCtt                                                                                                              <  1:3545396/148‑1 (MQ=255)
                                  cATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                             <  1:665042/148‑1 (MQ=255)
                                  cATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                             <  1:3799415/148‑1 (MQ=255)
                                  cATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCttt                                                                                                             >  1:2875870/1‑148 (MQ=255)
                                   aTGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTg                                                                                                            <  2:1863115/148‑1 (MQ=255)
                                    tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGt                                                                                                           <  1:2042103/148‑1 (MQ=255)
                                    tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGt                                                                                                           <  2:1996804/148‑1 (MQ=255)
                                    tGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGt                                                                                                           <  2:1946732/148‑1 (MQ=255)
                                       aTCTCCGCCATCAACAGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGAACATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCACGCAACTCATTGtttt                                                                                                        >  2:1612143/1‑148 (MQ=255)
                                         ctcCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCa                                                                                                      <  2:3361873/148‑1 (MQ=255)
                                           ccGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTATCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACg                                                                                                    >  1:3268728/1‑148 (MQ=255)
                                           ccGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACg                                                                                                    >  2:2985505/1‑148 (MQ=255)
                                            cGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACgg                                                                                                   <  1:798253/148‑1 (MQ=255)
                                            cGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACgg                                                                                                   <  1:2187492/148‑1 (MQ=255)
                                             gCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGa                                                                                                  <  1:2297368/148‑1 (MQ=255)
                                                 tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              <  2:3266360/148‑1 (MQ=255)
                                                 tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              <  1:2878088/148‑1 (MQ=255)
                                                 tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              >  2:2438345/1‑148 (MQ=255)
                                                 tCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTg                                                                                              <  1:2669391/148‑1 (MQ=255)
                                                  cAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGc                                                                                             >  1:3213649/1‑148 (MQ=255)
                                                      tGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaa                                                                                         <  2:257158/148‑1 (MQ=255)
                                                       gTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaat                                                                                        >  2:3762569/1‑148 (MQ=255)
                                                        ttGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                       <  1:187769/148‑1 (MQ=255)
                                                        ttGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                       <  2:2222071/148‑1 (MQ=255)
                                                        ttGGCCCTGCCGAGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                       <  1:777904/148‑1 (MQ=255)
                                                        ttGGCCCTGCCGAGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaata                                                                                       <  1:776711/148‑1 (MQ=255)
                                                         tGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTtaataa                                                                                      >  2:2732197/1‑148 (MQ=255)
                                                            cccTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATgg                                                                                   <  2:1687527/148‑1 (MQ=255)
                                                            cccTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATgg                                                                                   <  2:1487085/148‑1 (MQ=255)
                                                                 ccGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGtcgcgc                                                                              >  2:3505714/1‑148 (MQ=255)
                                                                 ccGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGgcgcgc                                                                              >  2:3505741/1‑148 (MQ=255)
                                                                  cgcgCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCa                                                                             <  2:1435543/148‑1 (MQ=255)
                                                                   gcgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAc                                                                            <  2:1215907/148‑1 (MQ=255)
                                                                   gcgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAc                                                                            <  1:505204/148‑1 (MQ=255)
                                                                    cgcCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCAcc                                                                           <  1:159741/148‑1 (MQ=255)
                                                                       cAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGc                                                                        >  1:2989251/1‑148 (MQ=255)
                                                                          agCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTAGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAg                                                                     >  2:2745671/1‑148 (MQ=255)
                                                                           gCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAgg                                                                    <  1:3181194/148‑1 (MQ=255)
                                                                              tCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCaa                                                                 <  1:3322291/148‑1 (MQ=255)
                                                                               ccGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCaaa                                                                <  1:2263680/148‑1 (MQ=255)
                                                                               ccGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCaaa                                                                >  2:2528209/1‑148 (MQ=255)
                                                                                  cttcttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTc                                                             <  1:2225481/148‑1 (MQ=255)
                                                                                  cttcttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTc                                                             >  2:3125339/1‑148 (MQ=255)
                                                                                   ttcttGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTcc                                                            >  1:3508265/1‑148 (MQ=255)
                                                                                         cgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTc                                                      <  1:1543034/148‑1 (MQ=255)
                                                                                         cgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTc                                                      <  1:2885886/148‑1 (MQ=255)
                                                                                         cgcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTc                                                      <  1:1619709/148‑1 (MQ=255)
                                                                                          gcCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCa                                                     <  2:1394980/148‑1 (MQ=255)
                                                                                            cGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAgg                                                   >  2:850794/1‑148 (MQ=255)
                                                                                              ttttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGt                                                 <  1:3714206/148‑1 (MQ=255)
                                                                                              ttttGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGt                                                 <  1:1414795/148‑1 (MQ=255)
                                                                                                  gCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTc                                             <  1:2735293/148‑1 (MQ=255)
                                                                                                         ccGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTtct                                      <  2:3291966/148‑1 (MQ=255)
                                                                                                           gATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCa                                    <  2:1736471/148‑1 (MQ=255)
                                                                                                             tCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAcc                                  >  2:2835277/1‑148 (MQ=255)
                                                                                                              cATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCAccc                                 >  2:2203714/1‑148 (MQ=255)
                                                                                                                tGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCgg                               >  1:494686/1‑148 (MQ=255)
                                                                                                                tGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAAGGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCgg                               <  2:759944/148‑1 (MQ=255)
                                                                                                                 gTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGt                              <  1:3079001/148‑1 (MQ=255)
                                                                                                                   tGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAc                            >  2:880369/1‑148 (MQ=255)
                                                                                                                    gATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACg                           <  2:3778261/148‑1 (MQ=255)
                                                                                                                    gATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACg                           <  2:3778489/148‑1 (MQ=255)
                                                                                                                     aTGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGt                          >  1:3633511/1‑148 (MQ=255)
                                                                                                                        tCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGa                       >  2:1750126/1‑148 (MQ=255)
                                                                                                                        tCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGa                       >  2:3639293/1‑148 (MQ=255)
                                                                                                                        tCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGa                       <  1:1033653/148‑1 (MQ=255)
                                                                                                                         cTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGAt                      >  2:264842/1‑148 (MQ=255)
                                                                                                                           gcgcCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATgg                    <  1:1782969/148‑1 (MQ=255)
                                                                                                                            cgcCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGt                   <  1:3780502/148‑1 (MQ=255)
                                                                                                                              ccAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGa                 <  1:413276/148‑1 (MQ=255)
                                                                                                                                aGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATa               <  1:1278883/148‑1 (MQ=255)
                                                                                                                                aGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATa               <  2:2941384/148‑1 (MQ=255)
                                                                                                                                 gCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATaa              <  1:2371073/148‑1 (MQ=255)
                                                                                                                                      gAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCa         <  1:3326381/148‑1 (MQ=255)
                                                                                                                                      gAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCa         <  1:3326362/148‑1 (MQ=255)
                                                                                                                                       aaGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCaa        >  2:2267191/1‑148 (MQ=255)
                                                                                                                                         gTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATACTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtg      <  1:3018339/148‑1 (MQ=255)
                                                                                                                                         gTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtg      <  2:3272281/148‑1 (MQ=255)
                                                                                                                                         gTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtg      <  1:3125339/148‑1 (MQ=255)
                                                                                                                                           tGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgtt    >  1:626374/1‑148 (MQ=255)
                                                                                                                                           tGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgtt    >  1:2220114/1‑148 (MQ=255)
                                                                                                                                             tCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTCTTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgttgt  >  2:1372813/1‑148 (MQ=255)
                                                                                                                                             tCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAtgttgt  >  2:8838/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGT  >  CP009273/2452310‑2452598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  >  CP009273/2452307‑2452601
                                                                                                                                                   |                                                                                                                                                   
GCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGT                                                                                                                                                   <  SRR19973069.2711442/150‑1 (MQ=60)
   CCATGAATAGCCGCGATAACCTGAATTGGCAAAGCATGAATCTCCGCCATCAACTGTTGGACCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTA                                                                                                                                                <  SRR19973069.2505007/150‑1 (MQ=60)
      TGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCG                                                                                                                                             >  SRR19973069.3754294/1‑150 (MQ=60)
      TGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCG                                                                                                                                             >  SRR19973069.1679923/1‑150 (MQ=60)
           AGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAC                                                                                                                                        <  SRR19973069.2162360/150‑1 (MQ=60)
           AGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAC                                                                                                                                        >  SRR19973069.1749718/1‑150 (MQ=60)
           AGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAC                                                                                                                                        >  SRR19973069.1748647/1‑150 (MQ=60)
           AGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGAC                                                                                                                                        <  SRR19973069.856448/150‑1 (MQ=60)
                CGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACA                                                                                                                                   <  SRR19973069.2844273/150‑1 (MQ=60)
                CGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACA                                                                                                                                   <  SRR19973069.2336868/150‑1 (MQ=60)
                  ATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACC                                                                                                                                 >  SRR19973069.3811598/1‑150 (MQ=60)
                    AACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCAC                                                                                                                               >  SRR19973069.3838431/1‑150 (MQ=60)
                       CTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCC                                                                                                                            >  SRR19973069.3539777/1‑150 (MQ=60)
                         GAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTC                                                                                                                          <  SRR19973069.180084/150‑1 (MQ=60)
                               ccacacccagacaccccgccatccactgggGGCCCTGCCGCGCCAGAGCTGCCGCTTCTTGCGCCGTTTTGCAGNTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACT                                                                                                                    <  SRR19973069.1948710/120‑1 (MQ=60)
                                CAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTC                                                                                                                   >  SRR19973069.1518555/1‑150 (MQ=60)
                                 AAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCCGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCT                                                                                                                  >  SRR19973069.3532718/1‑150 (MQ=60)
                                 AAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGACGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCT                                                                                                                  >  SRR19973069.3654132/1‑150 (MQ=60)
                                 AAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCT                                                                                                                  <  SRR19973069.450641/150‑1 (MQ=60)
                                  AAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTT                                                                                                                 >  SRR19973069.1799410/1‑150 (MQ=60)
                                  AAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTT                                                                                                                 >  SRR19973069.1800199/1‑150 (MQ=60)
                                   AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                <  SRR19973069.1547212/150‑1 (MQ=60)
                                   AGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTT                                                                                                                >  SRR19973069.2894739/1‑150 (MQ=60)
                                    GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                               <  SRR19973069.2351103/150‑1 (MQ=60)
                                    GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                               <  SRR19973069.3567553/150‑1 (MQ=60)
                                    GCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTG                                                                                                               <  SRR19973069.3568422/150‑1 (MQ=60)
                                     CATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGT                                                                                                              <  SRR19973069.669563/150‑1 (MQ=60)
                                     CATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGT                                                                                                              <  SRR19973069.3824028/150‑1 (MQ=60)
                                       TGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTT                                                                                                            <  SRR19973069.2056283/150‑1 (MQ=60)
                                          ATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCA                                                                                                         >  SRR19973069.43748/1‑150 (MQ=60)
                                            CTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTATCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACG                                                                                                       >  SRR19973069.3290137/1‑150 (MQ=60)
                                            CTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACG                                                                                                       <  SRR19973069.794693/150‑1 (MQ=60)
                                            CTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACG                                                                                                       >  SRR19973069.654810/1‑150 (MQ=60)
                                               CGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAG                                                                                                    <  SRR19973069.2202387/150‑1 (MQ=60)
                                               CGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAG                                                                                                    <  SRR19973069.741719/150‑1 (MQ=60)
                                               CGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAG                                                                                                    <  SRR19973069.804319/150‑1 (MQ=60)
                                               CGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAG                                                                                                    <  SRR19973069.803911/150‑1 (MQ=60)
                                                GCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGT                                                                                                   <  SRR19973069.373353/150‑1 (MQ=60)
                                                GCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGT                                                                                                   <  SRR19973069.782534/150‑1 (MQ=60)
                                                GCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGT                                                                                                   <  SRR19973069.2312974/150‑1 (MQ=60)
                                                 CCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTT                                                                                                  >  SRR19973069.3164005/1‑150 (MQ=60)
                                                   ATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGC                                                                                                >  SRR19973069.3234614/1‑150 (MQ=60)
                                                   ATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGC                                                                                                >  SRR19973069.228222/1‑150 (MQ=60)
                                                    TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                               <  SRR19973069.2687291/150‑1 (MQ=60)
                                                    TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                               <  SRR19973069.2896951/150‑1 (MQ=60)
                                                    TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                               <  SRR19973069.2896968/150‑1 (MQ=60)
                                                    TCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCT                                                                                               <  SRR19973069.2896980/150‑1 (MQ=60)
                                                      AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                             >  SRR19973069.3293625/1‑150 (MQ=60)
                                                      AACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTA                                                                                             >  SRR19973069.3293707/1‑150 (MQ=60)
                                                           TTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAAT                                                                                        <  SRR19973069.189314/150‑1 (MQ=60)
                                                           TTGGCCCTGCCGAGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAAT                                                                                        <  SRR19973069.783417/150‑1 (MQ=60)
                                                           TTGGCCCTGCCGAGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAAT                                                                                        <  SRR19973069.782217/150‑1 (MQ=60)
                                                                      GCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCT                                                                             <  SRR19973069.508817/150‑1 (MQ=60)
                                                                       CGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTG                                                                            <  SRR19973069.161136/150‑1 (MQ=60)
                                                                        GCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGC                                                                           >  SRR19973069.3009100/1‑150 (MQ=60)
                                                                              GCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCA                                                                     <  SRR19973069.3001938/150‑1 (MQ=60)
                                                                              GCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCA                                                                     <  SRR19973069.3201881/150‑1 (MQ=60)
                                                                               CTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAA                                                                    >  SRR19973069.2217630/1‑150 (MQ=60)
                                                                                 TCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAAC                                                                  <  SRR19973069.3344074/150‑1 (MQ=60)
                                                                                  CCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACT                                                                 <  SRR19973069.2279012/150‑1 (MQ=60)
                                                                                    GCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCC                                                               >  SRR19973069.3530836/1‑150 (MQ=60)
                                                                                     CTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCG                                                              <  SRR19973069.2240555/150‑1 (MQ=60)
                                                                                     CTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCG                                                              <  SRR19973069.2240523/150‑1 (MQ=60)
                                                                                     CTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCG                                                              >  SRR19973069.3747328/1‑150 (MQ=60)
                                                                                      TTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGC                                                             >  SRR19973069.3769158/1‑150 (MQ=60)
                                                                                        CTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCT                                                           >  SRR19973069.2793066/1‑150 (MQ=60)
                                                                                          TGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTC                                                         >  SRR19973069.456592/1‑150 (MQ=60)
                                                                                            CGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAG                                                       <  SRR19973069.1553605/150‑1 (MQ=60)
                                                                                            CGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAG                                                       >  SRR19973069.637603/1‑150 (MQ=60)
                                                                                            CGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAG                                                       <  SRR19973069.2904882/150‑1 (MQ=60)
                                                                                            CGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAG                                                       <  SRR19973069.1630631/150‑1 (MQ=60)
                                                                                            CGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAG                                                       <  SRR19973069.1630674/150‑1 (MQ=60)
                                                                                             GCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGG                                                      <  SRR19973069.2480094/150‑1 (MQ=60)
                                                                                                 TTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTAT                                                  <  SRR19973069.1424655/150‑1 (MQ=60)
                                                                                                 TTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTAT                                                  <  SRR19973069.3738462/150‑1 (MQ=60)
                                                                                                    TGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCA                                               <  SRR19973069.968825/150‑1 (MQ=60)
                                                                                                     GCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCAT                                              <  SRR19973069.2753595/150‑1 (MQ=60)
                                                                                                       AGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTT                                            >  SRR19973069.2299899/1‑150 (MQ=60)
                                                                                                        GTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATCATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTT                                           <  SRR19973069.3207433/150‑1 (MQ=60)
                                                                                                          TGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCT                                         >  SRR19973069.3271111/1‑150 (MQ=60)
                                                                                                           GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                        >  SRR19973069.3088837/1‑150 (MQ=60)
                                                                                                           GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                        >  SRR19973069.2893128/1‑150 (MQ=60)
                                                                                                           GCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTC                                        >  SRR19973069.2240441/1‑150 (MQ=60)
                                                                                                              GATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACC                                     >  SRR19973069.613737/1‑150 (MQ=60)
                                                                                                               ATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCC                                    >  SRR19973069.3519551/1‑150 (MQ=60)
                                                                                                               ATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCC                                    >  SRR19973069.1342846/1‑150 (MQ=60)
                                                                                                                 CATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGG                                  >  SRR19973069.498258/1‑150 (MQ=60)
                                                                                                                 CATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACACCGCCTTCAGGGTATTCATTTTCTCACCCGG                                  >  SRR19973069.498239/1‑150 (MQ=60)
                                                                                                                    GTTGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAC                               <  SRR19973069.3099319/150‑1 (MQ=60)
                                                                                                                      TGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTCTTCATTTTCTCACCCGGTACGT                             >  SRR19973069.1749696/1‑150 (MQ=60)
                                                                                                                      TGATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGT                             >  SRR19973069.3657267/1‑150 (MQ=60)
                                                                                                                        ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                           >  SRR19973069.1199599/1‑150 (MQ=60)
                                                                                                                        ATGTCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCG                           >  SRR19973069.2725016/1‑150 (MQ=60)
                                                                                                                           TCTGCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATG                        <  SRR19973069.1040820/150‑1 (MQ=60)
                                                                                                                              GCGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTG                     <  SRR19973069.1795526/150‑1 (MQ=60)
                                                                                                                               CGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGA                    <  SRR19973069.3805045/150‑1 (MQ=60)
                                                                                                                               CGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGA                    <  SRR19973069.1904203/150‑1 (MQ=60)
                                                                                                                               CGCCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGA                    <  SRR19973069.3135789/150‑1 (MQ=60)
                                                                                                                                 CCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGNTA                  <  SRR19973069.2385123/150‑1 (MQ=60)
                                                                                                                                 CCAGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATA                  <  SRR19973069.416303/150‑1 (MQ=60)
                                                                                                                                   AGCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAAC                <  SRR19973069.1287457/150‑1 (MQ=60)
                                                                                                                                    GCAATGAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACG               <  SRR19973069.2387160/150‑1 (MQ=60)
                                                                                                                                         GAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAT          <  SRR19973069.3348162/150‑1 (MQ=60)
                                                                                                                                         GAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAT          <  SRR19973069.3348149/150‑1 (MQ=60)
                                                                                                                                         GAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAT          <  SRR19973069.3348181/150‑1 (MQ=60)
                                                                                                                                         GAAGTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAAT          <  SRR19973069.3348165/150‑1 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATACTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       <  SRR19973069.3038363/150‑1 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       >  SRR19973069.630531/1‑150 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       <  SRR19973069.3145853/150‑1 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       <  SRR19973069.3038348/150‑1 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       >  SRR19973069.2235132/1‑150 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       <  SRR19973069.1595014/150‑1 (MQ=60)
                                                                                                                                            GTTGTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTT       <  SRR19973069.142983/150‑1 (MQ=60)
                                                                                                                                               GTCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTC    >  SRR19973069.1089655/1‑150 (MQ=60)
                                                                                                                                                TCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCC   <  SRR19973069.2525777/150‑1 (MQ=60)
                                                                                                                                                TCCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCC   <  SRR19973069.1792650/150‑1 (MQ=60)
                                                                                                                                                 CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  <  SRR19973069.3468663/150‑1 (MQ=60)
                                                                                                                                                 CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  <  SRR19973069.3468524/150‑1 (MQ=60)
                                                                                                                                                 CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  <  SRR19973069.3468462/150‑1 (MQ=60)
                                                                                                                                                 CCTGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  <  SRR19973069.315716/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
GCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAAACACCACGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCA  >  CP009273/2452307‑2452601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑