Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,770 C→A 16.8% V623V (GTG→GTT)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,7700CA16.8% 695.9 / 78.4 235V623V (GTG→GTT) fadJfused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (95/100);  new base A (16/24);  total (111/124)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

CCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTG  >  CP009273/2450625‑2450917
                                                                                                                                                 |                                                                                                                                                   
 cgcgCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATcaca                                                                                                                                                  <  2:399183/148‑1 (MQ=255)
 cgcgCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATcaca                                                                                                                                                  <  2:283677/148‑1 (MQ=255)
     ccGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACAcc                                                                                                                                              >  2:1731732/1‑148 (MQ=255)
       gagagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGt                                                                                                                                            <  1:1518358/148‑1 (MQ=255)
         gagaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTc                                                                                                                                          <  1:2289905/148‑1 (MQ=255)
          agaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCa                                                                                                                                         >  2:3131574/1‑148 (MQ=255)
           gaATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAg                                                                                                                                        >  2:2183385/1‑148 (MQ=255)
             aTCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCa                                                                                                                                      <  1:2734332/148‑1 (MQ=255)
               cGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAAc                                                                                                                                    <  1:3474631/148‑1 (MQ=255)
               cGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAAc                                                                                                                                    >  1:1403176/1‑148 (MQ=255)
                  tataGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtg                                                                                                                                 <  1:1180776/148‑1 (MQ=255)
                  tataGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtg                                                                                                                                 <  1:1181412/148‑1 (MQ=255)
                          aaCGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtgcgg                                                                                                                         <  1:3193253/148‑1 (MQ=255)
                          aaCGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCtgcggtgcgg                                                                                                                         <  1:1955294/148‑1 (MQ=255)
                            cGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGgag                                                                                                                       >  1:1015406/1‑148 (MQ=255)
                            cGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGgag                                                                                                                       >  1:1091679/1‑148 (MQ=255)
                              gTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAt                                                                                                                     <  1:218248/148‑1 (MQ=255)
                               tCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAtt                                                                                                                    >  2:1606988/1‑148 (MQ=255)
                                   ccGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGcc                                                                                                                >  2:1942449/1‑148 (MQ=255)
                                       gaAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTg                                                                                                            >  1:2251864/1‑148 (MQ=255)
                                        aaaTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGc                                                                                                           >  1:455395/1‑148 (MQ=255)
                                         aaTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGcc                                                                                                          <  1:704168/148‑1 (MQ=255)
                                         aaTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGcc                                                                                                          <  1:679933/148‑1 (MQ=255)
                                           tGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCt                                                                                                        <  2:1345439/148‑1 (MQ=255)
                                           tGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCt                                                                                                        >  2:2096734/1‑148 (MQ=255)
                                                gAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgt                                                                                                   >  1:1690251/1‑148 (MQ=255)
                                                 aaaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgtg                                                                                                  >  1:1127496/1‑148 (MQ=255)
                                                 aaaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTtgtgtg                                                                                                  <  2:1745449/148‑1 (MQ=255)
                                                  aaaCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGc                                                                                                 <  2:1101418/148‑1 (MQ=255)
                                                    aCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCa                                                                                               <  1:50300/148‑1 (MQ=255)
                                                      cAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAAt                                                                                             <  1:1891673/148‑1 (MQ=255)
                                                        aTGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCa                                                                                           >  2:3073935/1‑148 (MQ=255)
                                                        aTGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCa                                                                                           >  1:884782/1‑148 (MQ=255)
                                                             aaaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCggg                                                                                      <  1:2931691/148‑1 (MQ=255)
                                                             aaaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCggg                                                                                      <  1:1158793/148‑1 (MQ=255)
                                                              aaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGt                                                                                     >  2:2029521/1‑148 (MQ=255)
                                                              aaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGt                                                                                     <  2:1175850/148‑1 (MQ=255)
                                                              aaTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGt                                                                                     >  1:1714497/1‑148 (MQ=255)
                                                                tACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTaa                                                                                   >  2:3376297/1‑148 (MQ=255)
                                                                tACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTaa                                                                                   <  1:3131574/148‑1 (MQ=255)
                                                                tACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTaa                                                                                   <  1:735258/148‑1 (MQ=255)
                                                                  ccGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAAt                                                                                 <  1:2823670/148‑1 (MQ=255)
                                                                  ccGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAAt                                                                                 >  2:1688951/1‑148 (MQ=255)
                                                                  ccGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAAt                                                                                 >  2:3543317/1‑148 (MQ=255)
                                                                    gcgcCAATATCCCCGTCACGCACTCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATgg                                                                               <  2:185173/148‑1 (MQ=255)
                                                                    gcgcCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATgg                                                                               <  2:809116/148‑1 (MQ=255)
                                                                       ccAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCgg                                                                            <  1:3014909/148‑1 (MQ=255)
                                                                           tatCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATc                                                                        >  2:2973984/1‑148 (MQ=255)
                                                                            atCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGCGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCg                                                                       <  1:1842218/148‑1 (MQ=255)
                                                                              ccccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAc                                                                     <  2:952616/148‑1 (MQ=255)
                                                                               cccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGAcc                                                                    >  1:3254064/1‑148 (MQ=255)
                                                                                ccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCt                                                                   >  2:3496884/1‑148 (MQ=255)
                                                                                 cGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTg                                                                  <  1:454359/148‑1 (MQ=255)
                                                                                 cGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTg                                                                  >  2:542162/1‑148 (MQ=255)
                                                                                   tCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGtt                                                                >  1:2462659/1‑148 (MQ=255)
                                                                                    cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttt                                                               <  1:3551870/148‑1 (MQ=255)
                                                                                    cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttt                                                               <  1:1793915/148‑1 (MQ=255)
                                                                                    cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTAATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGttt                                                               <  1:2103683/148‑1 (MQ=255)
                                                                                      cgcacgcTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGatttt                                                             <  2:147254/148‑1 (MQ=255)
                                                                                          cgcTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCt                                                         <  1:3463772/148‑1 (MQ=255)
                                                                                            cTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCttt                                                       <  2:1659164/148‑1 (MQ=255)
                                                                                             tACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCtttt                                                      <  1:2550653/148‑1 (MQ=255)
                                                                                                ggATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACg                                                   >  1:606523/1‑148 (MQ=255)
                                                                                                ggATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACg                                                   <  1:933263/148‑1 (MQ=255)
                                                                                                  aTAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc                                                 <  2:3135254/148‑1 (MQ=255)
                                                                                                  aTAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc                                                 <  1:622492/148‑1 (MQ=255)
                                                                                                     aCCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCtt                                              <  1:332451/148‑1 (MQ=255)
                                                                                                      ccTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCttt                                             >  2:198575/1‑148 (MQ=255)
                                                                                                      ccTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCttt                                             >  2:2242957/1‑148 (MQ=255)
                                                                                                         gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                          >  2:1194826/1‑148 (MQ=255)
                                                                                                           tcatcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGAc                                        <  1:2211037/148‑1 (MQ=255)
                                                                                                            catcaACACAACGTACTGCTTCATTCAGCATCAACATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGAcc                                       >  2:2703244/1‑148 (MQ=255)
                                                                                                             atcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGTAAAAGGGCGGTATCTCCATGTTTTTTGGTTTTACGCCAtttatcacaa                                      >  2:577041/1‑141 (MQ=255)
                                                                                                                 acacAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCAGCGGTGAGGAGATTCTCCCCTGACCTTGTGTTCCAATCAGCGGCTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATaaa                                  >  1:1056431/1‑148 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 <  1:1263547/148‑1 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 <  1:2053588/148‑1 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 <  1:779472/148‑1 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 <  2:2881940/148‑1 (MQ=255)
                                                                                                                  cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAg                                 <  1:2968131/148‑1 (MQ=255)
                                                                                                                   acaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGa                                         >  1:1796824/1‑139 (MQ=255)
                                                                                                                   acaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGa                                <  1:559180/148‑1 (MQ=255)
                                                                                                                     aaCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATa                              >  2:764635/1‑148 (MQ=255)
                                                                                                                     aaCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATa                              >  1:1361215/1‑148 (MQ=255)
                                                                                                                      aCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAg                             <  2:2253410/148‑1 (MQ=255)
                                                                                                                          aCTGCTTCATTCAGCATCAACATCCCACACCGTTCATCAAGCTGCGGTGCGGAGATTCTCCCCTGCACTTGTGTGCCAATCAGCGGGTAACTGGCGGGATCGACCTGTTTTTTGCTTTTACGCACTTTCTGACCATAAAGATAGAAAc                         <  2:264005/148‑1 (MQ=255)
                                                                                                                          aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAAc                         >  2:2502502/1‑148 (MQ=255)
                                                                                                                           cTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAAcc                        <  2:1562516/148‑1 (MQ=255)
                                                                                                                               ttcattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCgg                    >  1:1245483/1‑148 (MQ=255)
                                                                                                                                 cattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGcc                  >  1:3147779/1‑148 (MQ=255)
                                                                                                                                  attcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCa                 >  1:2422603/1‑148 (MQ=255)
                                                                                                                                    tcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCAtt               <  1:797071/148‑1 (MQ=255)
                                                                                                                                     caGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCAttt              >  2:48220/1‑148 (MQ=255)
                                                                                                                                        cATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCAtttttt           <  2:2957253/148‑1 (MQ=255)
                                                                                                                                         aTCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTc          <  2:2421928/148‑1 (MQ=255)
                                                                                                                                          tCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCt         >  1:2891448/1‑148 (MQ=255)
                                                                                                                                            aaCATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGc       <  2:2589199/148‑1 (MQ=255)
                                                                                                                                             aCATAACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGcc      <  2:2331252/148‑1 (MQ=255)
                                                                                                                                               aTCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGTGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCtt    <  2:884782/148‑1 (MQ=255)
                                                                                                                                                tCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCttt   <  2:1797981/148‑1 (MQ=255)
                                                                                                                                                 cacacaCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTg  >  2:3118098/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTG  >  CP009273/2450625‑2450917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A