Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,576,325 C→A 93.2% E171D (GAG→GAT)  nudK ← GDP‑mannose pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,576,3250CA93.2% 817.6 / 47.1 273E171D (GAG→GAT) nudKGDP‑mannose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (14/5);  new base A (136/117);  total (150/123)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCG  >  CP009273/2576178‑2576469
                                                                                                                                                   |                                                                                                                                                
gTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAtatc                                                                                                                                                <  2:754959/150‑1 (MQ=255)
   tCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGcc                                                                                                                                             <  1:3071351/150‑1 (MQ=255)
   tCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGcc                                                                                                                                             <  1:3146725/150‑1 (MQ=255)
       cGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGtt                                                                                                                                         >  2:2108012/1‑150 (MQ=255)
       cGTTGCGCAGGTAGCGCGGCTCAATCGGATTGTTCGGATTGAGCCAATAATAAACACCGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTTCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATTTCGCCGGtt                                                                                                                                         <  2:4259211/150‑1 (MQ=255)
          tGCGCAGGTAGCGCGGCTAAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTg                                                                                                                                      >  2:716591/1‑150 (MQ=255)
           gcgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGa                                                                                                                                     <  2:1782457/150‑1 (MQ=255)
            cgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATACACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGat                                                                                                                                    <  2:4044717/150‑1 (MQ=255)
             gcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatc                                                                                                                                   <  2:3278474/150‑1 (MQ=255)
               aGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatcat                                                                                                                                 <  2:2747370/150‑1 (MQ=255)
               aGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatcat                                                                                                                                 >  2:3820973/1‑150 (MQ=255)
               aGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatcat                                                                                                                                 >  2:3819894/1‑150 (MQ=255)
                ggTCGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatcatc                                                                                                                                >  2:4522981/1‑150 (MQ=255)
                 gTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAtct                                                                                                                               <  2:2555080/150‑1 (MQ=255)
                        ggCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAgcgc                                                                                                                        >  1:1263537/1‑150 (MQ=255)
                        ggCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAgcgc                                                                                                                        >  1:2877902/1‑150 (MQ=255)
                         gCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGcc                                                                                                                       <  2:2391335/150‑1 (MQ=255)
                           tCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTg                                                                                                                     <  2:1584919/150‑1 (MQ=255)
                              aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCt                                                                                                                  >  1:262051/1‑150 (MQ=255)
                                 ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGaa                                                                                                               >  1:1176880/1‑150 (MQ=255)
                                      tGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCa                                                                                                          <  2:4319827/150‑1 (MQ=255)
                                      tGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCa                                                                                                          <  1:3252393/150‑1 (MQ=255)
                                      tGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCa                                                                                                          <  2:1144757/150‑1 (MQ=255)
                                       gTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAg                                                                                                         <  2:3025731/150‑1 (MQ=255)
                                         cGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCt                                                                                                       >  2:516668/1‑150 (MQ=255)
                                          ggATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCTGCTc                                                                                                      <  1:4514392/150‑1 (MQ=255)
                                          ggATTGAGCCAATAATAAACAACGAAATATAATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTc                                                                                                      >  2:2878165/1‑150 (MQ=255)
                                              tGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGc                                                                                                  >  1:2465953/1‑150 (MQ=255)
                                               gAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCa                                                                                                 >  2:1308815/1‑150 (MQ=255)
                                               gAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCa                                                                                                 <  1:4449875/150‑1 (MQ=255)
                                                aGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAc                                                                                                >  2:2949517/1‑150 (MQ=255)
                                                aGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAc                                                                                                >  1:4538104/1‑150 (MQ=255)
                                                   cAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTc                                                                                             >  2:3566186/1‑150 (MQ=255)
                                                           aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctt                                                                                     <  2:1110298/150‑1 (MQ=255)
                                                           aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctt                                                                                     <  1:528278/150‑1 (MQ=255)
                                                           aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctt                                                                                     <  1:4137227/150‑1 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                    >  2:332121/1‑150 (MQ=255)
                                                               acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatc                                                                                 >  2:2228821/1‑150 (MQ=255)
                                                               acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatc                                                                                 >  2:1933521/1‑150 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                               <  2:3519913/150‑1 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                               >  2:3387133/1‑150 (MQ=255)
                                                                  aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACtt                                                                                              >  1:730409/1‑134 (MQ=255)
                                                                  aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatcttc                                                                              <  2:2257457/150‑1 (MQ=255)
                                                                  aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctgcatcttc                                                                              >  2:4229702/1‑150 (MQ=255)
                                                                     tattatTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGac                                                                           <  1:4269930/150‑1 (MQ=255)
                                                                      attattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACAGTCACGTATATCGCCGGTTTTGATAATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTGCAATATCTTCATCTTCGact                                                                          >  2:4407725/1‑149 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                       >  1:4189714/1‑150 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                       >  1:856936/1‑150 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                       <  1:4053939/150‑1 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                       >  1:2136873/1‑150 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGAAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                       <  1:3673232/150‑1 (MQ=255)
                                                                           tGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGcc                                                                     <  1:403002/150‑1 (MQ=255)
                                                                           tGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAAAACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGATCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATATTCGACACCGcc                                                                     >  2:1740270/1‑150 (MQ=255)
                                                                             ttATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAc                                                                   >  2:4318785/1‑150 (MQ=255)
                                                                             ttATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAc                                                                   <  1:2227704/150‑1 (MQ=255)
                                                                              tATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAcc                                                                  >  2:1520574/1‑150 (MQ=255)
                                                                               aTTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCg                                                                 >  2:3236130/1‑150 (MQ=255)
                                                                               aTTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCg                                                                 >  2:3236809/1‑150 (MQ=255)
                                                                                tttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCgg                                                                >  1:2168526/1‑150 (MQ=255)
                                                                                 ttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGc                                                               >  2:2237666/1‑150 (MQ=255)
                                                                                         ccATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgcgc                                                       >  1:1968742/1‑150 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCt                                                    >  1:4374534/1‑150 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCt                                                    <  1:2467281/150‑1 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCt                                                    >  1:4374840/1‑150 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTg                                                   <  2:4088903/150‑1 (MQ=255)
                                                                                              aaaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGa                                                  <  1:2997579/150‑1 (MQ=255)
                                                                                               aaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAt                                                 >  2:2342350/1‑150 (MQ=255)
                                                                                               aaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAt                                                 >  1:3284579/1‑150 (MQ=255)
                                                                                                aTGTGACGTTTGCAAATAGTTAAGCAATAACGCCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAtt                                                >  1:3744850/1‑150 (MQ=255)
                                                                                                      cGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACt                                          >  1:3862195/1‑150 (MQ=255)
                                                                                                      cGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACt                                          >  1:1603090/1‑150 (MQ=255)
                                                                                                       gTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCAGGTGTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTg                                         <  2:119172/150‑1 (MQ=255)
                                                                                                        tttGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGt                                        <  1:1751357/150‑1 (MQ=255)
                                                                                                         ttGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTa                                       <  1:1035240/150‑1 (MQ=255)
                                                                                                         ttGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCAAGCGCCTGGCAGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTAGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTa                                       <  1:2932189/150‑1 (MQ=255)
                                                                                                            cAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTc                                    >  2:391825/1‑150 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCg                                   >  1:2020504/1‑150 (MQ=255)
                                                                                                              actAGTTAAGCAATAACAGCGGCGTACCGTCACTTATATCGCCGGTTTTGATCATCTCCAGCGCCGGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCgg                                  <  2:3674562/148‑1 (MQ=255)
                                                                                                                tAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCg                                >  1:2995651/1‑150 (MQ=255)
                                                                                                                tAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCg                                >  1:3361486/1‑150 (MQ=255)
                                                                                                                   ttAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATa                             <  2:933329/150‑1 (MQ=255)
                                                                                                                        cAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaaaaaa                        >  1:3543455/1‑150 (MQ=255)
                                                                                                                        cAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaaaaaa                        >  1:3542625/1‑150 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGt                      <  2:3803852/150‑1 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGt                      <  2:1544258/150‑1 (MQ=255)
                                                                                                                             acacCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGa                   >  1:1517522/1‑150 (MQ=255)
                                                                                                                             acacCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGa                   >  1:1520506/1‑150 (MQ=255)
                                                                                                                                  gTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGc              >  2:2019570/1‑150 (MQ=255)
                                                                                                                                   tCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCt             <  1:3819859/150‑1 (MQ=255)
                                                                                                                                   tCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCt             <  1:3820859/150‑1 (MQ=255)
                                                                                                                                      tACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAg          >  1:4113966/1‑150 (MQ=255)
                                                                                                                                      tACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAg          >  1:1528056/1‑150 (MQ=255)
                                                                                                                                       aCCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGt         >  2:4424826/1‑150 (MQ=255)
                                                                                                                                        ccGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTc        <  2:2299969/150‑1 (MQ=255)
                                                                                                                                         cGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTca       >  2:313306/1‑150 (MQ=255)
                                                                                                                                          gTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTcac      >  1:2296573/1‑150 (MQ=255)
                                                                                                                                           tCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTcaca     >  2:4133503/1‑150 (MQ=255)
                                                                                                                                            cACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCacac    <  2:4371564/150‑1 (MQ=255)
                                                                                                                                             aCGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACAcc   <  1:2237666/150‑1 (MQ=255)
                                                                                                                                              cGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCg  <  2:856995/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCG  >  CP009273/2576178‑2576469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGG  >  CP009273/2576180‑2576474
                                                                                                                                                 |                                                                                                                                                     
ATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGC                                                                                                                                                   >  SRR19973076.214115/1‑150 (MQ=60)
 TCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCC                                                                                                                                                  <  SRR19973076.3075810/150‑1 (MQ=60)
 TCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCC                                                                                                                                                  <  SRR19973076.3075813/150‑1 (MQ=60)
 TCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCC                                                                                                                                                  <  SRR19973076.3151123/150‑1 (MQ=60)
 TCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCC                                                                                                                                                  <  SRR19973076.3151290/150‑1 (MQ=60)
      GTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTT                                                                                                                                             >  SRR19973076.3411160/1‑150 (MQ=60)
             AGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAT                                                                                                                                      <  SRR19973076.3040031/150‑1 (MQ=60)
                   CGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAG                                                                                                                                <  SRR19973076.3345365/150‑1 (MQ=60)
                      GGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGC                                                                                                                             >  SRR19973076.2882072/1‑150 (MQ=60)
                      GGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGC                                                                                                                             >  SRR19973076.2231636/1‑150 (MQ=60)
                      GGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGC                                                                                                                             >  SRR19973076.1265411/1‑150 (MQ=60)
                      GGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGC                                                                                                                             >  SRR19973076.2884233/1‑150 (MQ=60)
                          CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                         <  SRR19973076.3954259/150‑1 (MQ=60)
                          CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                         <  SRR19973076.3954291/150‑1 (MQ=60)
                          CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                         <  SRR19973076.2924302/150‑1 (MQ=60)
                            ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                       >  SRR19973076.262487/1‑150 (MQ=60)
                              CGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGA                                                                                                                     >  SRR19973076.2070217/1‑150 (MQ=60)
                               GGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAA                                                                                                                    >  SRR19973076.1178625/1‑150 (MQ=60)
                               GGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAA                                                                                                                    >  SRR19973076.1178870/1‑150 (MQ=60)
                                    TGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCTGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCA                                                                                                               <  SRR19973076.3257220/150‑1 (MQ=60)
                                    TGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCA                                                                                                               <  SRR19973076.2653117/150‑1 (MQ=60)
                                    TGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCA                                                                                                               <  SRR19973076.3257122/150‑1 (MQ=60)
                                        GGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCTGCTC                                                                                                           <  SRR19973076.4521089/150‑1 (MQ=60)
                                        GGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTC                                                                                                           <  SRR19973076.3168898/150‑1 (MQ=60)
                                            TGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGC                                                                                                       >  SRR19973076.65199/1‑150 (MQ=60)
                                            TGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGC                                                                                                       >  SRR19973076.1615495/1‑150 (MQ=60)
                                            TGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGC                                                                                                       >  SRR19973076.2469534/1‑150 (MQ=60)
                                            TGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGC                                                                                                       >  SRR19973076.2470186/1‑150 (MQ=60)
                                             GAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTCAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCA                                                                                                      <  SRR19973076.4456376/150‑1 (MQ=60)
                                             GAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCA                                                                                                      <  SRR19973076.4456503/150‑1 (MQ=60)
                                             GAGACAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATACTTTAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCA                                                                                                      <  SRR19973076.4456382/150‑1 (MQ=60)
                                              AGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAC                                                                                                     >  SRR19973076.3782721/1‑150 (MQ=60)
                                              AGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAC                                                                                                     >  SRR19973076.2266264/1‑150 (MQ=60)
                                              AGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAC                                                                                                     >  SRR19973076.4544836/1‑150 (MQ=60)
                                              AGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAC                                                                                                     >  SRR19973076.2266265/1‑150 (MQ=60)
                                                  AATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCA                                                                                                 >  SRR19973076.2841832/1‑150 (MQ=60)
                                                         AACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTT                                                                                          <  SRR19973076.4143253/150‑1 (MQ=60)
                                                         AACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTT                                                                                          <  SRR19973076.4143342/150‑1 (MQ=60)
                                                         AACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTT                                                                                          <  SRR19973076.529117/150‑1 (MQ=60)
                                                            AACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAT                                                                                       <  SRR19973076.2140836/150‑1 (MQ=60)
                                                            AACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAT                                                                                       <  SRR19973076.3036606/150‑1 (MQ=60)
                                                            AACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAT                                                                                       <  SRR19973076.1501351/150‑1 (MQ=60)
                                                            AACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAT                                                                                       <  SRR19973076.752077/150‑1 (MQ=60)
                                                            AACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAT                                                                                       <  SRR19973076.752082/150‑1 (MQ=60)
                                                             ACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATC                                                                                      <  SRR19973076.2436677/150‑1 (MQ=60)
                                                             ACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATC                                                                                      <  SRR19973076.2436860/150‑1 (MQ=60)
                                                              CGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCT                                                                                     >  SRR19973076.1698288/1‑150 (MQ=60)
                                                                AAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTagatcggaagagcaca                                                                                   >  SRR19973076.731497/1‑134 (MQ=60)
                                                                   TATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGAC                                                                                <  SRR19973076.4276238/150‑1 (MQ=60)
                                                                    ATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACA                                                                               >  SRR19973076.1139321/1‑150 (MQ=60)
                                                                    ATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACATCAATATCTTCATCTTCGACA                                                                               >  SRR19973076.1577487/1‑150 (MQ=60)
                                                                      TATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACC                                                                             <  SRR19973076.4338465/150‑1 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.858256/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.858255/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.858197/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.4195899/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.4195827/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            <  SRR19973076.4171538/150‑1 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            <  SRR19973076.4059931/150‑1 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            <  SRR19973076.3678405/150‑1 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            >  SRR19973076.2139973/1‑150 (MQ=60)
                                                                       ATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGAAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCG                                                                            <  SRR19973076.3678596/150‑1 (MQ=60)
                                                                         TGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCC                                                                          <  SRR19973076.2594178/150‑1 (MQ=60)
                                                                         TGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCC                                                                          <  SRR19973076.40768/150‑1 (MQ=60)
                                                                         TGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCC                                                                          <  SRR19973076.40764/150‑1 (MQ=60)
                                                                         TGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCC                                                                          <  SRR19973076.403649/150‑1 (MQ=60)
                                                                           TTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAC                                                                        <  SRR19973076.2230908/150‑1 (MQ=60)
                                                                           TTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAC                                                                        <  SRR19973076.2229790/150‑1 (MQ=60)
                                                                             ATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCG                                                                      >  SRR19973076.4542272/1‑150 (MQ=60)
                                                                              TTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGG                                                                     >  SRR19973076.2171657/1‑150 (MQ=60)
                                                                                       CCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGC                                                            >  SRR19973076.1971604/1‑150 (MQ=60)
                                                                                       CCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGC                                                            >  SRR19973076.3761368/1‑150 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         <  SRR19973076.2470811/150‑1 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         <  SRR19973076.2470864/150‑1 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         >  SRR19973076.4381295/1‑150 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         >  SRR19973076.4381259/1‑150 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         >  SRR19973076.4380989/1‑150 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         <  SRR19973076.4201193/150‑1 (MQ=60)
                                                                                          TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                         <  SRR19973076.4201190/150‑1 (MQ=60)
                                                                                           TAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTG                                                        >  SRR19973076.4508082/1‑150 (MQ=60)
                                                                                           TAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTG                                                        >  SRR19973076.4508049/1‑150 (MQ=60)
                                                                                            AAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGA                                                       <  SRR19973076.3001939/150‑1 (MQ=60)
                                                                                             AATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAT                                                      <  SRR19973076.4546844/150‑1 (MQ=60)
                                                                                             AATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAT                                                      >  SRR19973076.3289349/1‑150 (MQ=60)
                                                                                              ATGTGACGTTTGCAAATAGTTAAGCAATAACGCCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATT                                                     >  SRR19973076.3750332/1‑150 (MQ=60)
                                                                                              ATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATT                                                     <  SRR19973076.1346944/150‑1 (MQ=60)
                                                                                              ATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATT                                                     >  SRR19973076.3749544/1‑150 (MQ=60)
                                                                                              ATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATT                                                     >  SRR19973076.3750085/1‑150 (MQ=60)
                                                                                                    CGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACT                                               >  SRR19973076.1605430/1‑150 (MQ=60)
                                                                                                    CGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACT                                               >  SRR19973076.1605433/1‑150 (MQ=60)
                                                                                                    CGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACT                                               >  SRR19973076.3867888/1‑150 (MQ=60)
                                                                                                      TTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGT                                             <  SRR19973076.1753893/150‑1 (MQ=60)
                                                                                                       TTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCCTCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTA                                            <  SRR19973076.2936661/150‑1 (MQ=60)
                                                                                                       TTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTA                                            <  SRR19973076.1036778/150‑1 (MQ=60)
                                                                                                       TTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCAAGCGCCTGGCAGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTAGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTA                                            <  SRR19973076.2936445/150‑1 (MQ=60)
                                                                                                           AAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCG                                        >  SRR19973076.2023485/1‑150 (MQ=60)
                                                                                                           AAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCG                                        >  SRR19973076.2023462/1‑150 (MQ=60)
                                                                                                            AATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGG                                       >  SRR19973076.3244024/1‑150 (MQ=60)
                                                                                                              TAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCG                                     >  SRR19973076.3366362/1‑150 (MQ=60)
                                                                                                              TAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCG                                     >  SRR19973076.4348229/1‑150 (MQ=60)
                                                                                                              TAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCG                                     >  SRR19973076.3366366/1‑150 (MQ=60)
                                                                                                              TAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCG                                     >  SRR19973076.3000009/1‑150 (MQ=60)
                                                                                                              TAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCG                                     >  SRR19973076.3000029/1‑150 (MQ=60)
                                                                                                                      CAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAA                             >  SRR19973076.3547771/1‑150 (MQ=60)
                                                                                                                      CAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAA                             >  SRR19973076.3548045/1‑150 (MQ=60)
                                                                                                                      CAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAA                             >  SRR19973076.3548603/1‑150 (MQ=60)
                                                                                                                      CAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAA                             >  SRR19973076.3548599/1‑150 (MQ=60)
                                                                                                                        ATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGT                           <  SRR19973076.2953805/150‑1 (MQ=60)
                                                                                                                          AACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGG                         <  SRR19973076.646453/150‑1 (MQ=60)
                                                                                                                           ACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGA                        >  SRR19973076.1519828/1‑150 (MQ=60)
                                                                                                                           ACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGA                        >  SRR19973076.1519754/1‑150 (MQ=60)
                                                                                                                           ACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGA                        >  SRR19973076.1522739/1‑150 (MQ=60)
                                                                                                                           ACACCGTCTTACCGTCACGTATCTCGCCGGGTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGA                        >  SRR19973076.1519815/1‑150 (MQ=60)
                                                                                                                                 TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                  <  SRR19973076.3825473/150‑1 (MQ=60)
                                                                                                                                 TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                  <  SRR19973076.3826588/150‑1 (MQ=60)
                                                                                                                                 TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                  <  SRR19973076.3826474/150‑1 (MQ=60)
                                                                                                                                 TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                  <  SRR19973076.3825540/150‑1 (MQ=60)
                                                                                                                                 TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                  <  SRR19973076.3825508/150‑1 (MQ=60)
                                                                                                                                  CTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTC                 >  SRR19973076.3297201/1‑150 (MQ=60)
                                                                                                                                  CTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTC                 <  SRR19973076.4529693/150‑1 (MQ=60)
                                                                                                                                   TTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCA                >  SRR19973076.4047434/1‑150 (MQ=60)
                                                                                                                                   TTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCA                >  SRR19973076.4047427/1‑150 (MQ=60)
                                                                                                                                    TACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAG               >  SRR19973076.4120044/1‑150 (MQ=60)
                                                                                                                                    TACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAG               >  SRR19973076.1864171/1‑150 (MQ=60)
                                                                                                                                    TACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAG               >  SRR19973076.1530294/1‑150 (MQ=60)
                                                                                                                                    TACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAG               >  SRR19973076.1530291/1‑150 (MQ=60)
                                                                                                                                    TACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAG               >  SRR19973076.1898675/1‑150 (MQ=60)
                                                                                                                                        GTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCAC           >  SRR19973076.2299862/1‑150 (MQ=60)
                                                                                                                                         TCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACA          >  SRR19973076.1233290/1‑150 (MQ=60)
                                                                                                                                         TCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACA          <  SRR19973076.2000223/150‑1 (MQ=60)
                                                                                                                                         TCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACA          >  SRR19973076.2220721/1‑150 (MQ=60)
                                                                                                                                           ACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACC        <  SRR19973076.2240881/150‑1 (MQ=60)
                                                                                                                                               ATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCT    >  SRR19973076.607726/1‑150 (MQ=60)
                                                                                                                                                 ATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGG  <  SRR19973076.3801553/150‑1 (MQ=60)
                                                                                                                                                 ATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGG  >  SRR19973076.265741/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
ATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGG  >  CP009273/2576180‑2576474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑