Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,576,325 C→A 100% E171D (GAG→GAT)  nudK ← GDP‑mannose pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,576,3250CA96.3% 808.4 / 18.4 246E171D (GAG→GAT) nudKGDP‑mannose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base A (123/114);  total (129/117)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCC  >  CP009273/2576178‑2576471
                                                                                                                                                   |                                                                                                                                                  
gTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAtatc                                                                                                                                                  >  2:3894667/1‑150 (MQ=255)
gTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAtatc                                                                                                                                                  >  2:1062884/1‑150 (MQ=255)
  aTCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGc                                                                                                                                                >  1:3716018/1‑150 (MQ=255)
     ttCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCgg                                                                                                                                             >  1:320178/1‑150 (MQ=255)
     ttCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCgg                                                                                                                                             <  1:1202049/150‑1 (MQ=255)
     ttCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCgg                                                                                                                                             >  1:658715/1‑150 (MQ=255)
       cGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGtt                                                                                                                                           <  1:2821295/150‑1 (MQ=255)
         ttGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGtttt                                                                                                                                         <  1:1798932/150‑1 (MQ=255)
           gcgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGa                                                                                                                                       <  2:1571644/150‑1 (MQ=255)
            cgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGat                                                                                                                                      <  1:1633161/150‑1 (MQ=255)
            cgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGat                                                                                                                                      <  1:3449158/150‑1 (MQ=255)
            cgcAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTGTTGat                                                                                                                                      >  1:151152/1‑150 (MQ=255)
              cAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatca                                                                                                                                    <  1:1270139/150‑1 (MQ=255)
                 gTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAtct                                                                                                                                 <  2:4469260/150‑1 (MQ=255)
                   aGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTcc                                                                                                                               >  1:3485412/1‑150 (MQ=255)
                    gcgcGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCa                                                                                                                              <  1:801549/150‑1 (MQ=255)
                     cgcgGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAg                                                                                                                             >  1:3258371/1‑150 (MQ=255)
                     cgcgGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAg                                                                                                                             >  1:3080054/1‑150 (MQ=255)
                     cgcgGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAg                                                                                                                             <  1:2606153/150‑1 (MQ=255)
                          cTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCt                                                                                                                        <  2:3069237/150‑1 (MQ=255)
                              aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCt                                                                                                                    >  1:2320679/1‑150 (MQ=255)
                              aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCt                                                                                                                    <  1:4111188/150‑1 (MQ=255)
                              aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCt                                                                                                                    <  1:1982918/150‑1 (MQ=255)
                              aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCt                                                                                                                    <  2:4316098/150‑1 (MQ=255)
                                 ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGaa                                                                                                                 >  1:3646264/1‑150 (MQ=255)
                                 ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGaa                                                                                                                 >  2:4049422/1‑150 (MQ=255)
                                 ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTATTGATCATCTCCAGCGCCTGGCTGaa                                                                                                                 <  2:1104911/150‑1 (MQ=255)
                                  gATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAAc                                                                                                                >  1:979037/1‑150 (MQ=255)
                                       gTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAg                                                                                                           >  2:2720844/1‑150 (MQ=255)
                                        tCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGc                                                                                                          <  1:3991454/150‑1 (MQ=255)
                                           gATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCg                                                                                                       <  2:2737200/150‑1 (MQ=255)
                                           gATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCg                                                                                                       <  1:1403681/150‑1 (MQ=255)
                                            aTTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGa                                                                                                      >  2:734444/1‑150 (MQ=255)
                                             ttGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAg                                                                                                     <  2:4116689/150‑1 (MQ=255)
                                             ttGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAg                                                                                                     <  2:3455168/150‑1 (MQ=255)
                                              tGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGc                                                                                                    <  1:4532673/150‑1 (MQ=255)
                                               gAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCa                                                                                                   >  1:3589935/1‑150 (MQ=255)
                                                aGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAc                                                                                                  >  2:2356520/1‑150 (MQ=255)
                                                  ccAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACtt                                                                                                >  2:2025922/1‑150 (MQ=255)
                                                   cAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTc                                                                                               >  1:4601724/1‑150 (MQ=255)
                                                      taataaACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAat                                                                                            <  1:3859223/150‑1 (MQ=255)
                                                           aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctt                                                                                       >  1:2158483/1‑150 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      >  2:210020/1‑150 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      >  2:1541278/1‑150 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      >  2:2958913/1‑150 (MQ=255)
                                                             caacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttca                                                                                     >  1:1299658/1‑150 (MQ=255)
                                                               acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatc                                                                                   >  2:68478/1‑150 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                                 <  2:3591711/150‑1 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                                 >  2:2521137/1‑150 (MQ=255)
                                                                        tattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACAcc                                                                          >  2:2923443/1‑150 (MQ=255)
                                                                          ttGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGc                                                                        >  1:56410/1‑150 (MQ=255)
                                                                           tGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGcc                                                                       >  2:1885500/1‑150 (MQ=255)
                                                                              tATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCAcc                                                                    <  1:375660/150‑1 (MQ=255)
                                                                               acttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCCCGAGCACTTCAATATCTTCATCTTCGACTCCGCCACCg                                                                   <  2:4616388/148‑1 (MQ=255)
                                                                                 ttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGc                                                                 >  1:4227946/1‑150 (MQ=255)
                                                                                 ttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGc                                                                 >  2:4287405/1‑150 (MQ=255)
                                                                                   ttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGt                                                               <  2:1306040/150‑1 (MQ=255)
                                                                                    tCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGtt                                                              <  1:3617210/150‑1 (MQ=255)
                                                                                     cAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTa                                                             >  1:1300353/1‑150 (MQ=255)
                                                                                     cAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTa                                                             >  1:115097/1‑150 (MQ=255)
                                                                                     cAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTa                                                             <  2:2025611/150‑1 (MQ=255)
                                                                                       gTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgc                                                           <  1:2697503/150‑1 (MQ=255)
                                                                                          cATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgcgcg                                                        >  1:631560/1‑150 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCTGTTTTGAACATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCATCATCTTCGACAACGCCACCGGCGTTATCGCGCt                                                      >  1:3604050/1‑150 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCt                                                      <  2:1779533/150‑1 (MQ=255)
                                                                                            ttAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTCCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCt                                                      <  1:2784924/150‑1 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTg                                                     >  2:1413991/1‑150 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCGCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTg                                                     >  2:1413942/1‑150 (MQ=255)
                                                                                              aaaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGa                                                    <  1:2407427/150‑1 (MQ=255)
                                                                                               aaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTTGCGCGCTGAt                                                   <  1:2422386/150‑1 (MQ=255)
                                                                                               aaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAt                                                   <  2:958540/150‑1 (MQ=255)
                                                                                                 tgtgACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTg                                                 >  2:2242324/1‑150 (MQ=255)
                                                                                                  gtgACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGt                                                >  1:1007251/1‑150 (MQ=255)
                                                                                                  gtgACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGt                                                <  1:1331090/150‑1 (MQ=255)
                                                                                                   tgACGTTTGCAAATAGTTAAGCAATAACACCGACTTACCGTCACGTATATCGCCGGTTATGATCATCTCCAGCGCCTGTCTGAACGGCAGCACGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTc                                               >  2:3144503/1‑150 (MQ=255)
                                                                                                     aCGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCAc                                             <  2:3415500/150‑1 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCg                                     >  2:2291489/1‑150 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCg                                     >  2:668904/1‑150 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCg                                     >  1:1890426/1‑150 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAACTCCAGCTCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGGTAGCGCGCTGATTGTCACTGTATTCg                                     >  2:335873/1‑150 (MQ=255)
                                                                                                              aaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCgg                                    <  1:1852377/150‑1 (MQ=255)
                                                                                                                tAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCg                                  <  2:3188549/150‑1 (MQ=255)
                                                                                                                 aGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGa                                 <  2:2093210/150‑1 (MQ=255)
                                                                                                                     aaGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaaa                             >  1:1085431/1‑150 (MQ=255)
                                                                                                                      aGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaaaa                            >  2:401744/1‑150 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGt                        <  1:4111491/150‑1 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGt                        >  1:2175768/1‑150 (MQ=255)
                                                                                                                            aaCACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTgg                      >  2:2597672/1‑150 (MQ=255)
                                                                                                                             acacCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCCCCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGa                     >  2:3506945/1‑150 (MQ=255)
                                                                                                                             acacCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGa                     <  1:2250877/150‑1 (MQ=255)
                                                                                                                               acCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGAtt                   <  2:282995/150‑1 (MQ=255)
                                                                                                                                ccGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTa                  <  2:1282647/150‑1 (MQ=255)
                                                                                                                                 cGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAg                 >  1:2628358/1‑150 (MQ=255)
                                                                                                                                 cGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAg                 >  2:3723094/1‑150 (MQ=255)
                                                                                                                                   tCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCt               <  1:499700/150‑1 (MQ=255)
                                                                                                                                           tCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTcaca       <  2:443501/150‑1 (MQ=255)
                                                                                                                                             aCGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACAcc     <  1:1864141/150‑1 (MQ=255)
                                                                                                                                              cGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCg    >  2:478432/1‑150 (MQ=255)
                                                                                                                                              cGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCg    >  2:2791509/1‑150 (MQ=255)
                                                                                                                                               gTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGc   >  2:583552/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCC  >  CP009273/2576178‑2576471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCT  >  CP009273/2576178‑2576472
                                                                                                                                                   |                                                                                                                                                   
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTC                                                                                                                                                   <  SRR19973073.91039/150‑1 (MQ=60)
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTC                                                                                                                                                   <  SRR19973073.91026/150‑1 (MQ=60)
  ATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGC                                                                                                                                                 >  SRR19973073.3723480/1‑150 (MQ=60)
     TTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGG                                                                                                                                              >  SRR19973073.660099/1‑150 (MQ=60)
     TTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGG                                                                                                                                              >  SRR19973073.320837/1‑150 (MQ=60)
     TTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGG                                                                                                                                              <  SRR19973073.1204543/150‑1 (MQ=60)
     TTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGG                                                                                                                                              >  SRR19973073.319481/1‑150 (MQ=60)
     TTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGG                                                                                                                                              >  SRR19973073.3971992/1‑150 (MQ=60)
       CGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTT                                                                                                                                            <  SRR19973073.2826846/150‑1 (MQ=60)
       CGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTT                                                                                                                                            <  SRR19973073.2827082/150‑1 (MQ=60)
         TTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTT                                                                                                                                          <  SRR19973073.1802647/150‑1 (MQ=60)
          TGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTG                                                                                                                                         <  SRR19973073.1420899/150‑1 (MQ=60)
          TGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTG                                                                                                                                         >  SRR19973073.1334650/1‑150 (MQ=60)
            CGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGAT                                                                                                                                       <  SRR19973073.3456030/150‑1 (MQ=60)
            CGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGAT                                                                                                                                       >  SRR19973073.3852894/1‑150 (MQ=60)
            CGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGAT                                                                                                                                       <  SRR19973073.1636538/150‑1 (MQ=60)
            CGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGAT                                                                                                                                       >  SRR19973073.2656284/1‑150 (MQ=60)
            CGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTGTTGAT                                                                                                                                       >  SRR19973073.151514/1‑150 (MQ=60)
              CAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCA                                                                                                                                     <  SRR19973073.1272799/150‑1 (MQ=60)
              CAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAAGAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCA                                                                                                                                     <  SRR19973073.3597998/150‑1 (MQ=60)
                   AGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCC                                                                                                                                >  SRR19973073.3492352/1‑150 (MQ=60)
                   AGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTAC                                                                                                                                >  SRR19973073.2340888/1‑150 (MQ=60)
                    GCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCA                                                                                                                               <  SRR19973073.803172/150‑1 (MQ=60)
                     CGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAG                                                                                                                              >  SRR19973073.3264965/1‑150 (MQ=60)
                     CGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAG                                                                                                                              >  SRR19973073.3086237/1‑150 (MQ=60)
                     CGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAG                                                                                                                              <  SRR19973073.2611522/150‑1 (MQ=60)
                       CGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCG                                                                                                                            >  SRR19973073.2423082/1‑150 (MQ=60)
                         GCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCC                                                                                                                          >  SRR19973073.2184653/1‑150 (MQ=60)
                         GCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCC                                                                                                                          >  SRR19973073.2185137/1‑150 (MQ=60)
                          CTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCT                                                                                                                         >  SRR19973073.4160844/1‑150 (MQ=60)
                            CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                       >  SRR19973073.4109358/1‑150 (MQ=60)
                            CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                       <  SRR19973073.4554420/150‑1 (MQ=60)
                            CAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGG                                                                                                                       >  SRR19973073.1595618/1‑150 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     >  SRR19973073.2325402/1‑150 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.2872728/150‑1 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.1910732/150‑1 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.1987026/150‑1 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.1987082/150‑1 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.4119472/150‑1 (MQ=60)
                              ATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCT                                                                                                                     <  SRR19973073.4119417/150‑1 (MQ=60)
                                 GGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAA                                                                                                                  >  SRR19973073.3653580/1‑150 (MQ=60)
                                  GATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAAC                                                                                                                 >  SRR19973073.981079/1‑150 (MQ=60)
                                     TTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGC                                                                                                              <  SRR19973073.1845882/150‑1 (MQ=60)
                                      TGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCA                                                                                                             <  SRR19973073.1818357/150‑1 (MQ=60)
                                        TCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGC                                                                                                           <  SRR19973073.3999410/150‑1 (MQ=60)
                                          GGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTC                                                                                                         <  SRR19973073.635155/150‑1 (MQ=60)
                                          GGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTC                                                                                                         <  SRR19973073.635269/150‑1 (MQ=60)
                                           GATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCG                                                                                                        >  SRR19973073.3736877/1‑150 (MQ=60)
                                           GATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCG                                                                                                        <  SRR19973073.1406645/150‑1 (MQ=60)
                                              TGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGC                                                                                                     <  SRR19973073.4541760/150‑1 (MQ=60)
                                               GAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCA                                                                                                    >  SRR19973073.3597134/1‑150 (MQ=60)
                                                   CAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTC                                                                                                >  SRR19973073.1740056/1‑150 (MQ=60)
                                                   CAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTC                                                                                                >  SRR19973073.4610915/1‑150 (MQ=60)
                                                     ATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAA                                                                                              <  SRR19973073.535144/150‑1 (MQ=60)
                                                      TAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAAT                                                                                             <  SRR19973073.3866868/150‑1 (MQ=60)
                                                        ATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATAT                                                                                           >  SRR19973073.275254/1‑150 (MQ=60)
                                                          AAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCT                                                                                         >  SRR19973073.1021007/1‑150 (MQ=60)
                                                           AACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTT                                                                                        >  SRR19973073.2162987/1‑150 (MQ=60)
                                                           AACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTT                                                                                        >  SRR19973073.3213396/1‑150 (MQ=60)
                                                            ACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTC                                                                                       >  SRR19973073.528568/1‑150 (MQ=60)
                                                            ACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTC                                                                                       >  SRR19973073.1241439/1‑150 (MQ=60)
                                                            ACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTC                                                                                       >  SRR19973073.1241458/1‑150 (MQ=60)
                                                             CAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCA                                                                                      >  SRR19973073.1302401/1‑150 (MQ=60)
                                                             CAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCA                                                                                      >  SRR19973073.1302422/1‑150 (MQ=60)
                                                             CAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTAGAGCACTTCAATATCTTCA                                                                                      >  SRR19973073.1302415/1‑150 (MQ=60)
                                                             CAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCA                                                                                      >  SRR19973073.368802/1‑150 (MQ=60)
                                                                     TATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGAC                                                                              >  SRR19973073.619112/1‑150 (MQ=60)
                                                                     TATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGAC                                                                              >  SRR19973073.669987/1‑150 (MQ=60)
                                                                        TATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACC                                                                           <  SRR19973073.4586748/150‑1 (MQ=60)
                                                                        TATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACC                                                                           <  SRR19973073.1118121/150‑1 (MQ=60)
                                                                          TTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGC                                                                         >  SRR19973073.56570/1‑150 (MQ=60)
                                                                              TATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACC                                                                     <  SRR19973073.376397/150‑1 (MQ=60)
                                                                               ATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCG                                                                    >  SRR19973073.1553937/1‑150 (MQ=60)
                                                                                 TTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGC                                                                  >  SRR19973073.4236326/1‑150 (MQ=60)
                                                                                    TCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTT                                                               >  SRR19973073.1886768/1‑150 (MQ=60)
                                                                                    TCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTT                                                               <  SRR19973073.3624477/150‑1 (MQ=60)
                                                                                     CAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTA                                                              >  SRR19973073.1303096/1‑150 (MQ=60)
                                                                                     CAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTA                                                              >  SRR19973073.115380/1‑150 (MQ=60)
                                                                                     CAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTA                                                              <  SRR19973073.2588610/150‑1 (MQ=60)
                                                                                     CAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTA                                                              <  SRR19973073.2799124/150‑1 (MQ=60)
                                                                                       GTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGC                                                            <  SRR19973073.2702938/150‑1 (MQ=60)
                                                                                       GTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGC                                                            <  SRR19973073.2703014/150‑1 (MQ=60)
                                                                                       GTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGC                                                            <  SRR19973073.3645958/150‑1 (MQ=60)
                                                                                       GTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAACTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGC                                                            >  SRR19973073.346476/1‑150 (MQ=60)
                                                                                          CATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCG                                                         >  SRR19973073.632910/1‑150 (MQ=60)
                                                                                            TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCTGTTTTGAACATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCATCATCTTCGACAACGCCACCGGCGTTATCGCGCT                                                       >  SRR19973073.3611281/1‑150 (MQ=60)
                                                                                            TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                       >  SRR19973073.3611306/1‑150 (MQ=60)
                                                                                            TTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTCCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCT                                                       <  SRR19973073.2790626/150‑1 (MQ=60)
                                                                                             TAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTG                                                      <  SRR19973073.2478896/150‑1 (MQ=60)
                                                                                             TAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTG                                                      >  SRR19973073.3402659/1‑150 (MQ=60)
                                                                                              AAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGA                                                     <  SRR19973073.2412382/150‑1 (MQ=60)
                                                                                               AATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTTGCGCGCTGAT                                                    <  SRR19973073.2427384/150‑1 (MQ=60)
                                                                                                ATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATT                                                   >  SRR19973073.369936/1‑150 (MQ=60)
                                                                                                  GTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGT                                                 >  SRR19973073.1009357/1‑150 (MQ=60)
                                                                                                  GTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGT                                                 <  SRR19973073.1333905/150‑1 (MQ=60)
                                                                                                  GTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGT                                                 <  SRR19973073.1333916/150‑1 (MQ=60)
                                                                                                     ACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCAC                                              <  SRR19973073.1348970/150‑1 (MQ=60)
                                                                                                      CGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACT                                             >  SRR19973073.494647/1‑150 (MQ=60)
                                                                                                      CGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACT                                             <  SRR19973073.4508450/150‑1 (MQ=60)
                                                                                                             AAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCG                                      >  SRR19973073.1894287/1‑150 (MQ=60)
                                                                                                              AATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGG                                     <  SRR19973073.1856181/150‑1 (MQ=60)
                                                                                                              AATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGG                                     <  SRR19973073.1856188/150‑1 (MQ=60)
                                                                                                                  GTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGAT                                 <  SRR19973073.3180942/150‑1 (MQ=60)
                                                                                                                  GTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGAT                                 <  SRR19973073.3616485/150‑1 (MQ=60)
                                                                                                                   TTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATA                                <  SRR19973073.2033479/150‑1 (MQ=60)
                                                                                                                    TAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAA                               >  SRR19973073.3572937/1‑150 (MQ=60)
                                                                                                                     AAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAA                              >  SRR19973073.1087747/1‑150 (MQ=60)
                                                                                                                     AAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAA                              >  SRR19973073.1087669/1‑150 (MQ=60)
                                                                                                                        CAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAA                           >  SRR19973073.234368/1‑150 (MQ=60)
                                                                                                                          ATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGT                         <  SRR19973073.4119721/150‑1 (MQ=60)
                                                                                                                          ATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGT                         >  SRR19973073.2180300/1‑150 (MQ=60)
                                                                                                                          ATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGT                         >  SRR19973073.2180311/1‑150 (MQ=60)
                                                                                                                           TAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTG                        >  SRR19973073.261948/1‑150 (MQ=60)
                                                                                                                             ACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGA                      <  SRR19973073.2255504/150‑1 (MQ=60)
                                                                                                                              CACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGAT                     <  SRR19973073.4063405/150‑1 (MQ=60)
                                                                                                                                 CGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAG                  >  SRR19973073.2633759/1‑150 (MQ=60)
                                                                                                                                   TCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCT                <  SRR19973073.500771/150‑1 (MQ=60)
                                                                                                                                    CTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTC               <  SRR19973073.1288870/150‑1 (MQ=60)
                                                                                                                                    CTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTC               <  SRR19973073.1169460/150‑1 (MQ=60)
                                                                                                                                        CCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGTGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTC           <  SRR19973073.4186488/150‑1 (MQ=60)
                                                                                                                                          GTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTTGCTGAACTTCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCAC         <  SRR19973073.2284954/150‑1 (MQ=60)
                                                                                                                                          GTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCAC         <  SRR19973073.329418/150‑1 (MQ=60)
                                                                                                                                             ACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACC      <  SRR19973073.3823884/150‑1 (MQ=60)
                                                                                                                                             ACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACC      <  SRR19973073.1867962/150‑1 (MQ=60)
                                                                                                                                             ACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACC      <  SRR19973073.1868500/150‑1 (MQ=60)
                                                                                                                                                 ATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCT  <  SRR19973073.292314/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCT  >  CP009273/2576178‑2576472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑