Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,068,135 G→T 11.2% F140L (TTC→TTA)  yggD ← MtlR family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,068,1350GT11.2% 915.5 / 60.5 268F140L (TTC→TTA) yggDMtlR family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (128/110);  new base T (16/14);  total (144/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 272 total aligned reads displayed.

AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAA  >  CP009273/3067986‑3068282
                                                                                                                                                     |                                                                                                                                                   
 aTCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGgaga                                                                                                                                                    <  1:4407820/150‑1 (MQ=255)
  tctcTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGaa                                                                                                                                                   <  1:3947521/150‑1 (MQ=255)
  tctcTTCGTTCTGGTACTGTGCCTGGATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGaa                                                                                                                                                   <  1:419947/150‑1 (MQ=255)
   ctctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGaaa                                                                                                                                                  >  1:2173805/1‑150 (MQ=255)
    tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                                                                                                 >  2:913044/1‑150 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATc                                                                                                                                                <  1:2810040/150‑1 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATc                                                                                                                                                >  1:2169540/1‑150 (MQ=255)
      ttCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCa                                                                                                                                               <  1:603479/150‑1 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                            >  1:3590650/1‑150 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                            <  1:3666963/150‑1 (MQ=255)
             tGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACt                                                                                                                                        <  2:2880/150‑1 (MQ=255)
               gTACTGTGCCTGTATCTTCAGCCCATTCACCGTAAATTCAATATTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTc                                                                                                                                      >  1:2740700/1‑150 (MQ=255)
                tACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCa                                                                                                                                     <  2:700158/150‑1 (MQ=255)
                 aCTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCAt                                                                                                                                    >  1:2377388/1‑150 (MQ=255)
                   tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCg                                                                                                                                  <  2:2256131/150‑1 (MQ=255)
                    gtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGc                                                                                                                                 >  1:1503046/1‑150 (MQ=255)
                      gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCtt                                                                                                                               <  2:3503917/150‑1 (MQ=255)
                        cTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtttt                                                                                                                             <  2:3849918/150‑1 (MQ=255)
                         tGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                                                                                                            >  1:3256320/1‑150 (MQ=255)
                         tGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                                                                                                            <  1:353286/150‑1 (MQ=255)
                           tATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAAGTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAAc                                                                                                                          <  2:3902611/150‑1 (MQ=255)
                             tCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgc                                                                                                                        <  2:169821/150‑1 (MQ=255)
                               ttCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGt                                                                                                                      >  1:1318475/1‑150 (MQ=255)
                                 cAGCCCATTAACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCa                                                                                                                    >  2:2133760/1‑150 (MQ=255)
                                  aGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAt                                                                                                                   >  2:2017743/1‑150 (MQ=255)
                                    cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATag                                                                                                                 >  1:1918035/1‑150 (MQ=255)
                                      cATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATagag                                                                                                               >  1:2309965/1‑150 (MQ=255)
                                      cATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATagag                                                                                                               >  1:2309483/1‑150 (MQ=255)
                                        ttCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCa                                                                                                             >  1:4039759/1‑150 (MQ=255)
                                          cACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATc                                                                                                           <  1:4033427/150‑1 (MQ=255)
                                           aCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCg                                                                                                          <  2:2485188/150‑1 (MQ=255)
                                             cGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCa                                                                                                        <  2:3246668/150‑1 (MQ=255)
                                                 aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                    >  1:1126736/1‑150 (MQ=255)
                                                  aTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCg                                                                                                   <  2:3819485/150‑1 (MQ=255)
                                                  aTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCg                                                                                                   <  1:1688341/150‑1 (MQ=255)
                                                    tCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCggg                                                                                                 >  1:3089134/1‑150 (MQ=255)
                                                       aTTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTa                                                                                              >  1:800538/1‑150 (MQ=255)
                                                         tttCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATg                                                                                            >  1:2151624/1‑150 (MQ=255)
                                                           tCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACAATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCt                                                                                          >  2:1400561/1‑150 (MQ=255)
                                                            cACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTa                                                                                         <  1:4333142/150‑1 (MQ=255)
                                                            cACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTa                                                                                         >  1:488572/1‑150 (MQ=255)
                                                              cGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTAtt                                                                                       >  2:3462389/1‑150 (MQ=255)
                                                               ggCGTTTATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTTAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTg                                                                                      <  2:170561/150‑1 (MQ=255)
                                                               ggCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTg                                                                                      >  1:3053376/1‑150 (MQ=255)
                                                                 cGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc                                                                                    >  2:3743359/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  2:1025486/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  1:3173980/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  2:2028662/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  2:4276455/1‑150 (MQ=255)
                                                                    tAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGtt                                                                                 <  2:3378035/150‑1 (MQ=255)
                                                                       ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCt                                                                              >  2:66303/1‑150 (MQ=255)
                                                                       ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCt                                                                              >  2:3894713/1‑150 (MQ=255)
                                                                       ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCt                                                                              <  2:3763072/150‑1 (MQ=255)
                                                                        tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                             >  2:4408776/1‑150 (MQ=255)
                                                                        tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                             <  2:3863485/150‑1 (MQ=255)
                                                                           tttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATa                                                                          <  2:3078127/150‑1 (MQ=255)
                                                                            ttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaa                                                                         >  1:2767465/1‑150 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                        <  2:258932/150‑1 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                        <  2:2302434/150‑1 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                        <  2:190322/150‑1 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                        >  2:503528/1‑150 (MQ=255)
                                                                               aGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAAt                                                                      <  2:1456135/150‑1 (MQ=255)
                                                                                ggtggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATc                                                                     <  2:3269057/150‑1 (MQ=255)
                                                                                ggtggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATc                                                                     <  2:2073526/150‑1 (MQ=255)
                                                                                  tggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATccc                                                                   <  2:941396/150‑1 (MQ=255)
                                                                                  tggtCGTGACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATccc                                                                   <  2:3667360/150‑1 (MQ=255)
                                                                                       gTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtc                                                              >  1:899832/1‑150 (MQ=255)
                                                                                       gTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtc                                                              >  1:2019234/1‑150 (MQ=255)
                                                                                       gTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtc                                                              >  2:2749331/1‑150 (MQ=255)
                                                                                          aCTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatg                                                           <  2:1111147/150‑1 (MQ=255)
                                                                                           cTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgt                                                          >  1:3630913/1‑150 (MQ=255)
                                                                                               cAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATc                                                      >  2:3578070/1‑150 (MQ=255)
                                                                                                 gCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCgg                                                    <  2:1775117/150‑1 (MQ=255)
                                                                                                  cGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGc                                                   >  2:268783/1‑150 (MQ=255)
                                                                                                  cGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGc                                                   <  2:3525695/150‑1 (MQ=255)
                                                                                                     cAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa                                                >  1:2558294/1‑150 (MQ=255)
                                                                                                     cAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa                                                <  2:2678020/150‑1 (MQ=255)
                                                                                                      aGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                               <  1:3491477/150‑1 (MQ=255)
                                                                                                      aGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                               <  1:3491543/150‑1 (MQ=255)
                                                                                                      aGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                               >  1:2248950/1‑150 (MQ=255)
                                                                                                        cgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc                                             >  2:1347947/1‑150 (MQ=255)
                                                                                                         gcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCg                                            >  1:3794756/1‑150 (MQ=255)
                                                                                                            gTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGc                                         <  2:4151190/150‑1 (MQ=255)
                                                                                                            gTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGc                                         >  1:688619/1‑150 (MQ=255)
                                                                                                            gTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGc                                         >  1:689036/1‑150 (MQ=255)
                                                                                                                tGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGttt                                     >  1:3013530/1‑150 (MQ=255)
                                                                                                                    cATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATc                                 >  2:2355877/1‑150 (MQ=255)
                                                                                                                            tAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgt                         >  2:2013508/1‑150 (MQ=255)
                                                                                                                            tAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgt                         >  2:700692/1‑150 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                       <  2:1890116/150‑1 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                       >  1:4173188/1‑150 (MQ=255)
                                                                                                                                 cgcgCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAgg                    >  1:2595180/1‑150 (MQ=255)
                                                                                                                                   cgcCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTa                  >  1:3436583/1‑150 (MQ=255)
                                                                                                                                         tGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTTTTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCATATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCa            <  1:3894666/150‑1 (MQ=255)
                                                                                                                                         tGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCa            <  1:2615821/150‑1 (MQ=255)
                                                                                                                                         tGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCa            <  1:3894713/150‑1 (MQ=255)
                                                                                                                                         tGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCa            >  1:1710214/1‑150 (MQ=255)
                                                                                                                                           aCCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGa          <  1:1000889/150‑1 (MQ=255)
                                                                                                                                              aGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACt       <  2:1901661/150‑1 (MQ=255)
                                                                                                                                                   gagaAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaa  >  1:3352827/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAA  >  CP009273/3067986‑3068282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A