Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,068,135 G→T 42.2% F140L (TTC→TTA)  yggD ← MtlR family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,068,1350GT42.2% 265.9 / inf 270F140L (TTC→TTA) yggDMtlR family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (81/75);  new base T (80/34);  total (161/109)
Fisher's exact test for biased strand distribution p-value = 2.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 275 total aligned reads displayed.

AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAA  >  CP009273/3067986‑3068282
                                                                                                                                                     |                                                                                                                                                   
aaTCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGgag                                                                                                                                                     >  2:2646763/1‑150 (MQ=255)
aaTCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGgag                                                                                                                                                     <  2:2213779/150‑1 (MQ=255)
   ctctTCGTTATGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATaaa                                                                                                                                                  >  2:1238089/1‑150 (MQ=255)
    tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                                                                                                 >  2:3015217/1‑150 (MQ=255)
    tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                                                                                                 >  1:1782482/1‑150 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATc                                                                                                                                                >  2:3502447/1‑150 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATc                                                                                                                                                >  2:2519954/1‑150 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATc                                                                                                                                                <  2:3072479/150‑1 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                            <  2:3217937/150‑1 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                            <  2:3750452/150‑1 (MQ=255)
            cTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAAc                                                                                                                                         >  2:131422/1‑150 (MQ=255)
            cTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAAc                                                                                                                                         >  1:1355543/1‑150 (MQ=255)
              ggTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACtt                                                                                                                                       >  2:898126/1‑150 (MQ=255)
                 aCTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCAt                                                                                                                                    >  1:3533595/1‑150 (MQ=255)
                  cTGTGACTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTTCTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCTGACGTAGAAATCAGCGAACTTCATc                                                                                                                                   <  2:3686932/150‑1 (MQ=255)
                   tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCg                                                                                                                                  >  2:1291317/1‑150 (MQ=255)
                   tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCg                                                                                                                                  >  1:2149843/1‑150 (MQ=255)
                        cTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtttt                                                                                                                             <  2:1937394/150‑1 (MQ=255)
                             tCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgc                                                                                                                        >  2:4030043/1‑150 (MQ=255)
                               ttCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGt                                                                                                                      <  2:3891825/150‑1 (MQ=255)
                                tCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTc                                                                                                                     >  1:2528436/1‑150 (MQ=255)
                                  aGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAt                                                                                                                   >  1:2437566/1‑150 (MQ=255)
                                   gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATa                                                                                                                  >  2:2374169/1‑150 (MQ=255)
                                   gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATa                                                                                                                  >  2:292444/1‑150 (MQ=255)
                                   gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATa                                                                                                                  <  1:2087292/150‑1 (MQ=255)
                                    cccATTCTCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATag                                                                                                                 <  2:638040/150‑1 (MQ=255)
                                    cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATag                                                                                                                 <  2:2345669/150‑1 (MQ=255)
                                     ccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAACGAACTTCATCGCTTTTAACGCGTCATaga                                                                                                                >  1:1154076/1‑150 (MQ=255)
                                      cATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATagag                                                                                                               <  2:3485768/150‑1 (MQ=255)
                                      cATTCACCGTTAATTCAATTTTCACGGCGTGAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATagag                                                                                                               >  2:1587160/1‑150 (MQ=255)
                                           aCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCg                                                                                                          <  2:2917033/150‑1 (MQ=255)
                                               ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                                                                                                      <  2:110360/150‑1 (MQ=255)
                                               ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                                                                                                      >  1:2892969/1‑150 (MQ=255)
                                               ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                                                                                                      <  1:888789/150‑1 (MQ=255)
                                                tAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTg                                                                                                     >  1:3351561/1‑150 (MQ=255)
                                                 aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                    >  1:95303/1‑150 (MQ=255)
                                                 aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                    <  1:471645/150‑1 (MQ=255)
                                                 aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAAAGAGCATCGCATTGc                                                                                                    >  1:1825570/1‑150 (MQ=255)
                                                   ttCAATTTTCACGGCGTTAATTCCATTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCG‑TTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCggg                                                                                                 <  1:1756329/150‑1 (MQ=255)
                                                     cAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGt                                                                                                <  2:1782599/150‑1 (MQ=255)
                                                      aaTTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGtt                                                                                               >  1:1667738/1‑150 (MQ=255)
                                                           tCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCt                                                                                          <  1:4065077/150‑1 (MQ=255)
                                                             aCGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTAt                                                                                        <  2:4078143/150‑1 (MQ=255)
                                                              cGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTAtt                                                                                       <  2:1946121/150‑1 (MQ=255)
                                                                 cGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc                                                                                    <  2:4167458/150‑1 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  1:3273881/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  2:1322228/1‑150 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                   >  2:1533075/1‑150 (MQ=255)
                                                                     aaTTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTg                                                                                >  1:1906674/1‑150 (MQ=255)
                                                                        tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                             >  2:3400063/1‑150 (MQ=255)
                                                                        tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                             >  1:480792/1‑150 (MQ=255)
                                                                          cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGAt                                                                           <  1:3719612/150‑1 (MQ=255)
                                                                          cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGAt                                                                           >  2:1393080/1‑150 (MQ=255)
                                                                          cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCATTTAACGCGACATAGAGCATCGCAATGCGGGTTATGCTATTGACGTTGCTGAt                                                                           >  1:4005200/1‑150 (MQ=255)
                                                                           tttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATa                                                                          <  1:3199940/150‑1 (MQ=255)
                                                                           tttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATa                                                                          <  1:2210302/150‑1 (MQ=255)
                                                                           tttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATa                                                                          <  2:1905740/150‑1 (MQ=255)
                                                                            ttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaa                                                                         <  2:1616078/150‑1 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCCGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAAGTTGCTGATaaa                                                                        >  2:3005073/1‑150 (MQ=255)
                                                                              aaGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaaa                                                                       >  2:1647404/1‑150 (MQ=255)
                                                                               tggtggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTGTAACGCGTCATAGAGCATCGCAGTGCGGGTTATGCGATTGACGTTGCTGATAAAAt                                                                      >  2:2676913/2‑150 (MQ=255)
                                                                                      cGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                               >  2:3186542/1‑150 (MQ=255)
                                                                                      cGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                               >  1:1204927/1‑150 (MQ=255)
                                                                                      cGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                               >  1:775589/1‑150 (MQ=255)
                                                                                       gTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAATCGCGCCTCTGACCAGACGGATAAATCAGCGACCTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGATATAGACGTTGCTGATAAAATCCCAGgtc                                                              >  2:557753/1‑150 (MQ=255)
                                                                                       gTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtc                                                              >  1:3105850/1‑150 (MQ=255)
                                                                                        ttACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtca                                                             >  2:3852922/1‑150 (MQ=255)
                                                                                             gCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgtca                                                        <  1:2751980/150‑1 (MQ=255)
                                                                                             gCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCGTTTAACGCGGCAGAGAGCATCGCATTGCGGGGTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgtca                                                        >  1:1951332/1‑150 (MQ=255)
                                                                                               cAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATc                                                      <  2:3769007/150‑1 (MQ=255)
                                                                                               cAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATc                                                      <  1:2867019/150‑1 (MQ=255)
                                                                                                    tCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTCTGCTAGTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaa                                                 >  2:2566909/1‑150 (MQ=255)
                                                                                                     cAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa                                                >  1:3060452/1‑150 (MQ=255)
                                                                                                      aGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCGTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                               >  1:140354/1‑150 (MQ=255)
                                                                                                       gcgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATc                                              <  1:693218/150‑1 (MQ=255)
                                                                                                         gcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCg                                            >  2:1343865/1‑150 (MQ=255)
                                                                                                            gTTTTGACCATTCCGCTAAAACGCGCCTCTNACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGc                                         >  1:3859596/1‑150 (MQ=255)
                                                                                                            gTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCGTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAACCGGGc                                         >  2:2862102/1‑150 (MQ=255)
                                                                                                               ttGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGtt                                      <  2:82533/150‑1 (MQ=255)
                                                                                                                   ccATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCAt                                  <  2:2786729/150‑1 (MQ=255)
                                                                                                                   ccATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCAt                                  <  1:19906/150‑1 (MQ=255)
                                                                                                                   ccATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCAt                                  >  2:976872/1‑150 (MQ=255)
                                                                                                                    cATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATc                                 >  1:1568865/1‑150 (MQ=255)
                                                                                                                      ttCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCt                               <  1:2195027/150‑1 (MQ=255)
                                                                                                                         cGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgtt                            >  2:1082555/1‑150 (MQ=255)
                                                                                                                         cGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgtt                            <  2:3688929/150‑1 (MQ=255)
                                                                                                                          gCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttc                           <  1:3482892/150‑1 (MQ=255)
                                                                                                                           cTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcg                          <  1:3957793/150‑1 (MQ=255)
                                                                                                                             aaaaCGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgtt                        <  1:1611542/150‑1 (MQ=255)
                                                                                                                             aaaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgtt                        >  1:144811/1‑150 (MQ=255)
                                                                                                                                        ctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGcc             >  1:2151986/1‑150 (MQ=255)
                                                                                                                                         tGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCa            <  2:1264979/150‑1 (MQ=255)
                                                                                                                                          gACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAg           >  1:3630695/1‑150 (MQ=255)
                                                                                                                                             cAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc        >  2:1998839/1‑150 (MQ=255)
                                                                                                                                             cAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc        >  2:3586609/1‑150 (MQ=255)
                                                                                                                                              aGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACt       >  1:2293734/1‑150 (MQ=255)
                                                                                                                                              aGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACt       >  1:3152404/1‑150 (MQ=255)
                                                                                                                                                aCGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTgg     <  2:3319862/150‑1 (MQ=255)
                                                                                                                                                 cGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATAGGAAAATCCGGGCGTTTAATCCTGTTAGTTCATGTAATGCCAGAACTggg    <  2:2852542/150‑1 (MQ=255)
                                                                                                                                                  ggAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGa   <  1:1842191/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAA  >  CP009273/3067986‑3068282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A