Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,068,135 G→T 37.8% F140L (TTC→TTA)  yggD ← MtlR family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,068,1350GT37.8% 305.4 / 266.6 246F140L (TTC→TTA) yggDMtlR family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (76/77);  new base T (26/67);  total (102/144)
Fisher's exact test for biased strand distribution p-value = 8.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 249 total aligned reads displayed.

AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAA  >  CP009273/3067986‑3068284
                                                                                                                                                     |                                                                                                                                                     
  tctcTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAATGTGGTCGTTACTGACAGCGGCAGCGCCGTTTTTTCCAATCAGCAAAAAACCCCCTCAGAACACACCGAGaa                                                                                                                                                     >  1:819464/1‑150 (MQ=255)
  tctcTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATaa                                                                                                                                                     <  1:4451412/150‑1 (MQ=255)
    tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                                                                                                   <  1:1109560/150‑1 (MQ=255)
     ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATc                                                                                                                                                  >  2:1969282/1‑150 (MQ=255)
      ttCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCa                                                                                                                                                 <  2:2704881/150‑1 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                              <  2:2719634/150‑1 (MQ=255)
         gTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                                                                                                              <  2:3894115/150‑1 (MQ=255)
          ttCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTACGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGa                                                                                                                                             >  1:588246/1‑150 (MQ=255)
          ttCTGGTACTGTGCCTGTATCTTCACCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGa                                                                                                                                             <  1:1185241/150‑1 (MQ=255)
             tGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACt                                                                                                                                          <  2:2101761/150‑1 (MQ=255)
             tGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACt                                                                                                                                          >  2:3301150/1‑150 (MQ=255)
                 aCTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCAt                                                                                                                                      <  2:339510/150‑1 (MQ=255)
                    gtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGc                                                                                                                                   >  2:2575687/1‑150 (MQ=255)
                    gtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTACGCTAAAACGCGCCTCTTACCAGACGGAGAAATCAGCGAACATCATAGc                                                                                                                                   >  1:1849105/1‑150 (MQ=255)
                      gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCtt                                                                                                                                 <  2:3777669/150‑1 (MQ=255)
                      gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCtt                                                                                                                                 <  2:3777755/150‑1 (MQ=255)
                        cTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtttt                                                                                                                               <  2:1304077/150‑1 (MQ=255)
                          gTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTaa                                                                                                                             <  2:4723492/150‑1 (MQ=255)
                           tataTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAAc                                                                                                                            <  1:2408787/150‑1 (MQ=255)
                           tATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAc                                                                                                                            <  1:3862219/150‑1 (MQ=255)
                              cTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAAcgcg                                                                                                                         >  1:2323459/1‑150 (MQ=255)
                              cTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgcg                                                                                                                         >  1:722549/1‑150 (MQ=255)
                              cTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgcg                                                                                                                         <  2:1988577/150‑1 (MQ=255)
                                tCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTc                                                                                                                       >  2:2837775/1‑150 (MQ=255)
                                 cAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCa                                                                                                                      <  1:2169599/150‑1 (MQ=255)
                                     ccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATaga                                                                                                                  <  1:4661685/150‑1 (MQ=255)
                                       aTTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGc                                                                                                                >  1:759876/1‑150 (MQ=255)
                                       aTTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGc                                                                                                                <  1:2204616/150‑1 (MQ=255)
                                         tCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCAt                                                                                                              <  1:2533355/150‑1 (MQ=255)
                                           aCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGTCCATTACGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTAATCGCGTTTAACGCGTCATAGAGCAtag                                                                                                            >  1:510878/1‑148 (MQ=255)
                                           aCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGTCCATTACGCTAAAAAGCGCCTCTGTCCAGACGGAGAAAAAAGCGAACATCATCGCTTTTAACGCGTCATAGAGCATCg                                                                                                            >  1:2902998/1‑150 (MQ=255)
                                               ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                                                                                                        <  1:3344779/150‑1 (MQ=255)
                                               ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAATCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAAGCGTCATAGAGCATCGCAtt                                                                                                        >  1:1809036/1‑150 (MQ=255)
                                                 aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                      >  2:3030506/1‑150 (MQ=255)
                                                     cAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGt                                                                                                  >  2:3989185/1‑150 (MQ=255)
                                                       aTTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTa                                                                                                <  1:4494709/150‑1 (MQ=255)
                                                           tCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCt                                                                                            >  2:4457387/1‑150 (MQ=255)
                                                            cACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTa                                                                                           <  2:3009354/150‑1 (MQ=255)
                                                               ggCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTg                                                                                        <  2:1763904/150‑1 (MQ=255)
                                                               ggCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCCTTGCGGGTTATGCTATTg                                                                                        >  2:467132/1‑150 (MQ=255)
                                                                 cGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc                                                                                      <  1:3825500/150‑1 (MQ=255)
                                                                 cGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc                                                                                      <  1:1899664/150‑1 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                     <  1:4109166/150‑1 (MQ=255)
                                                                  gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                     <  1:1366885/150‑1 (MQ=255)
                                                                      aTTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGc                                                                                 >  2:3760885/1‑150 (MQ=255)
                                                                        tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                               <  1:4684643/150‑1 (MQ=255)
                                                                          cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTTACCCTTCCGCTAAAACCCGCCTCATACCAGACCGAGAAAACAGCGAACATCATCGCTTTTTAAGCGTCATATAGCATAGCATTGCGGGTTATGCTAATTACGTTGGTgtt                                                                             >  1:3616713/1‑148 (MQ=255)
                                                                          cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAAGCGCCTCTGACCAGACGGAGAAATCAGCGAAATTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGATAATTACGTTTCTGAt                                                                             >  1:1444609/1‑150 (MQ=255)
                                                                           tttAAGGTGGTAGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATa                                                                            >  1:2106953/1‑150 (MQ=255)
                                                                            ttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaa                                                                           <  1:3626742/150‑1 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                          >  2:2448300/1‑150 (MQ=255)
                                                                             tAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATaaa                                                                          <  2:4262774/150‑1 (MQ=255)
                                                                                  tggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATccc                                                                     <  2:518462/150‑1 (MQ=255)
                                                                                      cGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                                 <  2:2057208/150‑1 (MQ=255)
                                                                                      cGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAGTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                                 >  2:3562475/1‑150 (MQ=255)
                                                                                        ttACTGCCAGCGTCAGCGCCGTTTTTACCATTACGCTAAAACGCGCCTATGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTAATATATCAACGCATTGCGGGTTATTCTATTGACTTTGCTGATAAAATACCAGgtca                                                               >  2:4035475/1‑150 (MQ=255)
                                                                                         tACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcat                                                              <  1:883085/150‑1 (MQ=255)
                                                                                         tACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcat                                                              >  2:899132/1‑150 (MQ=255)
                                                                                         tACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcat                                                              <  2:2667562/150‑1 (MQ=255)
                                                                                           cTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgt                                                            <  1:1450514/150‑1 (MQ=255)
                                                                                           cTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgt                                                            <  1:3527761/150‑1 (MQ=255)
                                                                                              ccAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgtcat                                                         <  2:4479226/150‑1 (MQ=255)
                                                                                               cAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATc                                                        <  1:2566747/150‑1 (MQ=255)
                                                                                                aGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCg                                                       <  2:3821045/150‑1 (MQ=255)
                                                                                                  cGTCAGCGCCGTTTTGACCATACCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGc                                                     >  2:2254298/1‑150 (MQ=255)
                                                                                                   gTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCa                                                    >  1:2340082/1‑150 (MQ=255)
                                                                                                   gTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCa                                                    <  1:2222405/150‑1 (MQ=255)
                                                                                                   gTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCa                                                    <  1:1248208/150‑1 (MQ=255)
                                                                                                         gcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCg                                              <  1:651534/150‑1 (MQ=255)
                                                                                                         gcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCg                                              >  1:1330859/1‑150 (MQ=255)
                                                                                                          ccGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCgg                                             >  1:2667246/1‑150 (MQ=255)
                                                                                                           cGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCggg                                            >  2:3668056/1‑150 (MQ=255)
                                                                                                             ttttGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCg                                          <  1:995305/150‑1 (MQ=255)
                                                                                                                tGACCATTCCGCTAACACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGttt                                       <  1:2960102/150‑1 (MQ=255)
                                                                                                                tGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGttt                                       <  1:1992667/150‑1 (MQ=255)
                                                                                                                   ccATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCAt                                    <  2:759876/150‑1 (MQ=255)
                                                                                                                     aTTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATcc                                  <  1:1734395/150‑1 (MQ=255)
                                                                                                                      ttCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCt                                 >  2:1678583/1‑150 (MQ=255)
                                                                                                                       tCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTg                                >  2:2608075/1‑150 (MQ=255)
                                                                                                                         cGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgtt                              <  1:454210/150‑1 (MQ=255)
                                                                                                                           cTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcg                            >  2:4719906/1‑150 (MQ=255)
                                                                                                                             aaaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgtt                          >  2:1372914/1‑150 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                         <  2:928920/150‑1 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                         >  2:3786970/1‑150 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                         >  1:3193459/1‑150 (MQ=255)
                                                                                                                              aaaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTGTCATCCTgttcgttc                         >  1:2032351/1‑150 (MQ=255)
                                                                                                                               aaCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCa                        <  2:239396/150‑1 (MQ=255)
                                                                                                                                 cgcgCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAgg                      <  1:3468660/150‑1 (MQ=255)
                                                                                                                                   cgcCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTa                    >  1:1051025/1‑150 (MQ=255)
                                                                                                                                      ctctGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATg                 <  2:2814546/150‑1 (MQ=255)
                                                                                                                                      ctctGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATg                 <  2:2323459/150‑1 (MQ=255)
                                                                                                                                        ctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGcc               <  2:722549/150‑1 (MQ=255)
                                                                                                                                          gACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAg             <  2:45506/150‑1 (MQ=255)
                                                                                                                                           aCCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGa            >  2:347376/1‑150 (MQ=255)
                                                                                                                                            ccAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGaa           >  2:411493/1‑150 (MQ=255)
                                                                                                                                             cAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc          >  2:1541687/1‑150 (MQ=255)
                                                                                                                                             cAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc          <  2:1167629/150‑1 (MQ=255)
                                                                                                                                             cAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAAc          <  1:4293603/150‑1 (MQ=255)
                                                                                                                                               gACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTg        <  2:2612634/150‑1 (MQ=255)
                                                                                                                                                   gagaAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGaa    <  1:1024562/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAA  >  CP009273/3067986‑3068284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A