Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 396,883 A→G 66.4% Q14Q (CAA→CAG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273396,8830AG66.4% 307.4 / 231.2 226Q14Q (CAA→CAG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base A (46/30);  new base G (90/60);  total (136/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

ATTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396736‑397031
                                                                                                                                                   |                                                                                                                                                    
atTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATAAATACATGAAAAATAACATTGCTGAGTGGTTATTTAAGCTTGCCAaaaa                                                                                                                                                    >  2:1937733/1‑150 (MQ=255)
     cgcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAaagaag                                                                                                                                               >  2:2527995/1‑150 (MQ=255)
        aaaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaagaag                                                                                                                                            >  2:1802985/1‑150 (MQ=255)
         aaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAagaagaaga                                                                                                                                           <  1:3569987/150‑1 (MQ=255)
            tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                        <  1:3294929/150‑1 (MQ=255)
            tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                        >  1:3744498/1‑150 (MQ=255)
            tATTTCCTTTGTAATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAAAATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTTTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                        <  2:3395275/150‑1 (MQ=255)
               ttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGaa                                                                                                                                     >  2:1123045/1‑150 (MQ=255)
                tcatttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGaaa                                                                                                                                    <  2:2672941/147‑1 (MQ=255)
                tCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGaaa                                                                                                                                    >  1:813051/1‑150 (MQ=255)
                  cTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGa                                                                                                                                  >  1:2224479/1‑150 (MQ=255)
                   tttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGaa                                                                                                                                 >  1:2859057/1‑150 (MQ=255)
                   tttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGaa                                                                                                                                 >  1:959427/1‑150 (MQ=255)
                   tttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGaa                                                                                                                                 >  1:3963996/1‑150 (MQ=255)
                    ttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAAc                                                                                                                                <  1:3696946/150‑1 (MQ=255)
                      ttaaTAAAAATAATACTTCCAGACACTATTAAGTTGTTAAACATAATGTTAACTTCTCCATACTTTTGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                              <  2:3635887/147‑1 (MQ=255)
                      gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                              >  1:136824/1‑150 (MQ=255)
                       tCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgt                                                                                                                             <  1:703370/150‑1 (MQ=255)
                       tCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgt                                                                                                                             <  2:3553234/150‑1 (MQ=255)
                          tAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACtgtgtg                                                                                                                          >  2:576257/1‑150 (MQ=255)
                             aaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                                                       >  2:3243497/1‑150 (MQ=255)
                             aaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                                                       >  1:2798270/1‑150 (MQ=255)
                              aataatACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCa                                                                                                                      >  2:2879827/1‑150 (MQ=255)
                               ataataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAg                                                                                                                     >  1:2534791/1‑150 (MQ=255)
                               ataataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAg                                                                                                                     >  1:3494617/1‑150 (MQ=255)
                                taataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                                                    <  1:1558176/150‑1 (MQ=255)
                                taataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                                                    >  1:4434401/1‑150 (MQ=255)
                                  ataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTa                                                                                                                  <  1:399799/150‑1 (MQ=255)
                                    aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                                <  2:2621215/150‑1 (MQ=255)
                                    aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                                >  2:4421920/1‑150 (MQ=255)
                                        ccAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCt                                                                                                            <  1:3660229/150‑1 (MQ=255)
                                         cAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCtt                                                                                                           >  1:3792695/1‑150 (MQ=255)
                                          aGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                          >  1:1970099/1‑150 (MQ=255)
                                             cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                       <  1:1711731/150‑1 (MQ=255)
                                               cTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                     >  2:4097076/1‑150 (MQ=255)
                                                tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                    >  1:4425531/1‑150 (MQ=255)
                                                 aTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAtt                                                                                                   >  1:1167741/1‑150 (MQ=255)
                                                    aaGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATc                                                                                                <  2:3092687/150‑1 (MQ=255)
                                                     aGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCg                                                                                               <  2:3436060/150‑1 (MQ=255)
                                                      gTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGt                                                                                              <  2:606756/150‑1 (MQ=255)
                                                       ttGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTc                                                                                             <  1:3110196/150‑1 (MQ=255)
                                                       ttGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTc                                                                                             <  1:4496927/150‑1 (MQ=255)
                                                        tgtgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                                                            <  2:378183/150‑1 (MQ=255)
                                                            aaaCATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGc                                                                                        >  1:700040/1‑150 (MQ=255)
                                                              aCATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCaa                                                                                      <  2:1649948/150‑1 (MQ=255)
                                                              aCATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCaa                                                                                      >  2:3067923/1‑150 (MQ=255)
                                                               cATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAAt                                                                                     >  2:1187097/1‑150 (MQ=255)
                                                                  aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                                  >  1:3878596/1‑150 (MQ=255)
                                                                   aTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCtt                                                                                 <  2:3348575/150‑1 (MQ=255)
                                                                       tAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                             >  2:1786111/1‑150 (MQ=255)
                                                                           ttCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAatat                                                                         <  2:2250301/150‑1 (MQ=255)
                                                                            tctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                                                        <  2:3445017/150‑1 (MQ=255)
                                                                               ccATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgcg                                                                     >  1:1090849/1‑150 (MQ=255)
                                                                                cATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgcgc                                                                    >  1:3718653/1‑150 (MQ=255)
                                                                                 aTACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCa                                                                   >  2:3375997/1‑150 (MQ=255)
                                                                                   aCTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaa                                                                 >  2:797370/1‑150 (MQ=255)
                                                                                    cTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaaa                                                                >  2:446154/1‑150 (MQ=255)
                                                                                    cTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaaa                                                                >  2:4287498/1‑150 (MQ=255)
                                                                                     tttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAt                                                               <  1:3524941/150‑1 (MQ=255)
                                                                                        ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                            >  1:874490/1‑150 (MQ=255)
                                                                                         gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                           <  1:4327322/150‑1 (MQ=255)
                                                                                          aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCa                                                          >  1:2461180/1‑150 (MQ=255)
                                                                                           tAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCaa                                                         >  1:1746123/1‑150 (MQ=255)
                                                                                            aaGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAc                                                        <  2:3759598/150‑1 (MQ=255)
                                                                                              ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                      >  1:605832/1‑150 (MQ=255)
                                                                                              ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                      <  1:2456246/150‑1 (MQ=255)
                                                                                               gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGc                                                     <  1:3651722/150‑1 (MQ=255)
                                                                                               gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGc                                                     <  1:2504302/150‑1 (MQ=255)
                                                                                               gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGc                                                     >  1:2807666/1‑150 (MQ=255)
                                                                                                  aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                                  >  1:1036670/1‑150 (MQ=255)
                                                                                                   tACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGtt                                                 <  1:1520519/150‑1 (MQ=255)
                                                                                                   tACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGtt                                                 <  1:2402439/150‑1 (MQ=255)
                                                                                                    aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttg                                                <  2:2928737/150‑1 (MQ=255)
                                                                                                    aCAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttg                                                <  2:3963971/150‑1 (MQ=255)
                                                                                                     cAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga                                               >  2:3569056/1‑150 (MQ=255)
                                                                                                       gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAGGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt                                             >  2:2044316/1‑150 (MQ=255)
                                                                                                          aTGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                          >  1:2960127/1‑150 (MQ=255)
                                                                                                            gAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCa                                        >  2:2047143/1‑150 (MQ=255)
                                                                                                              aaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAgg                                      >  1:2600324/1‑150 (MQ=255)
                                                                                                              aaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAgg                                      <  1:3182498/150‑1 (MQ=255)
                                                                                                                aaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTa                                    >  1:1794627/1‑150 (MQ=255)
                                                                                                                      aTTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgggg                              <  2:650764/150‑1 (MQ=255)
                                                                                                                      aTTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgggg                              <  2:1920464/150‑1 (MQ=255)
                                                                                                                       ttGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggg                             >  1:2767239/1‑150 (MQ=255)
                                                                                                                        tGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgc                            <  2:1567445/150‑1 (MQ=255)
                                                                                                                        tGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgc                            <  1:2054884/150‑1 (MQ=255)
                                                                                                                           tGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACCGCGGTTGATTGATCAGGTAGAGGGGGCGCt                         >  2:1020345/1‑150 (MQ=255)
                                                                                                                            gAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTg                        >  1:3500958/1‑150 (MQ=255)
                                                                                                                             aGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGt                       >  2:3995231/1‑150 (MQ=255)
                                                                                                                               ttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAc                     >  1:3239748/1‑150 (MQ=255)
                                                                                                                                 gttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa                   >  1:3915022/1‑150 (MQ=255)
                                                                                                                                 gttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGa                   >  1:150095/1‑150 (MQ=255)
                                                                                                                                  ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                  <  2:3744498/150‑1 (MQ=255)
                                                                                                                                  ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                  >  2:2721782/1‑150 (MQ=255)
                                                                                                                                  ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                  >  2:2721673/1‑150 (MQ=255)
                                                                                                                                  ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                  >  1:232735/1‑150 (MQ=255)
                                                                                                                                       tAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa             >  1:4461015/1‑150 (MQ=255)
                                                                                                                                       tAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa             >  2:541057/1‑150 (MQ=255)
                                                                                                                                        aaGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg            <  1:1716499/150‑1 (MQ=255)
                                                                                                                                        aaGCGTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg            <  2:3163086/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396736‑397031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396741‑397031
                                                                                                                                              |                                                                                                                                                    
CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAG                                                                                                                                               >  SRR19973080.3286909/1‑150 (MQ=60)
    AAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGA                                                                                                                                           <  SRR19973080.3589739/150‑1 (MQ=60)
       TATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTC                                                                                                                                        <  SRR19973080.3312983/150‑1 (MQ=60)
       TATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTC                                                                                                                                        >  SRR19973080.3764956/1‑150 (MQ=60)
           TCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAA                                                                                                                                    >  SRR19973080.818079/1‑150 (MQ=60)
           TCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAA                                                                                                                                    >  SRR19973080.817857/1‑150 (MQ=60)
             CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                  >  SRR19973080.2236741/1‑150 (MQ=60)
             CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                  >  SRR19973080.4524305/1‑150 (MQ=60)
              TTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAA                                                                                                                                 >  SRR19973080.2874729/1‑150 (MQ=60)
              TTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAA                                                                                                                                 >  SRR19973080.965248/1‑150 (MQ=60)
              TTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAA                                                                                                                                 >  SRR19973080.2944666/1‑150 (MQ=60)
              TTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAA                                                                                                                                 >  SRR19973080.3985796/1‑150 (MQ=60)
              TTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAA                                                                                                                                 >  SRR19973080.3985821/1‑150 (MQ=60)
               TTGTCATAAAAATAATACTTCCAGAAACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAAC                                                                                                                                <  SRR19973080.3717258/150‑1 (MQ=60)
                TGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACT                                                                                                                               >  SRR19973080.248603/1‑150 (MQ=60)
                 GTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTG                                                                                                                              >  SRR19973080.137967/1‑150 (MQ=60)
                 GTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTG                                                                                                                              >  SRR19973080.2010574/1‑150 (MQ=60)
                  TCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGT                                                                                                                             <  SRR19973080.707471/150‑1 (MQ=60)
                   CATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTG                                                                                                                            >  SRR19973080.1087751/1‑150 (MQ=60)
                        AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                       >  SRR19973080.2813698/1‑150 (MQ=60)
                        AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                       >  SRR19973080.2813694/1‑150 (MQ=60)
                        AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGGTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                       >  SRR19973080.2811748/1‑150 (MQ=60)
                          ATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAG                                                                                                                     >  SRR19973080.2548729/1‑150 (MQ=60)
                          ATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAG                                                                                                                     >  SRR19973080.2548708/1‑150 (MQ=60)
                          ATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAG                                                                                                                     >  SRR19973080.3513892/1‑150 (MQ=60)
                           TAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGG                                                                                                                    <  SRR19973080.1567224/150‑1 (MQ=60)
                           TAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGG                                                                                                                    >  SRR19973080.4458483/1‑150 (MQ=60)
                           TAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGG                                                                                                                    >  SRR19973080.4458549/1‑150 (MQ=60)
                             ATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTA                                                                                                                  >  SRR19973080.3518615/1‑150 (MQ=60)
                             ATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTA                                                                                                                  <  SRR19973080.2678828/150‑1 (MQ=60)
                             ATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTA                                                                                                                  <  SRR19973080.402182/150‑1 (MQ=60)
                              TACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAG                                                                                                                 >  SRR19973080.3856633/1‑150 (MQ=60)
                                 TTCCAGACACTATGAATTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAG                                                                                                              <  SRR19973080.394648/150‑1 (MQ=60)
                                  TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                             <  SRR19973080.2879711/150‑1 (MQ=60)
                                   CCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCT                                                                                                            <  SRR19973080.3680413/150‑1 (MQ=60)
                                   CCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCT                                                                                                            <  SRR19973080.3678952/150‑1 (MQ=60)
                                    CAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTT                                                                                                           >  SRR19973080.3813345/1‑150 (MQ=60)
                                    CAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTT                                                                                                           >  SRR19973080.3813349/1‑150 (MQ=60)
                                     AGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTT                                                                                                          <  SRR19973080.2082600/150‑1 (MQ=60)
                                     AGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTT                                                                                                          >  SRR19973080.1981282/1‑150 (MQ=60)
                                      GACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTG                                                                                                         >  SRR19973080.3536672/1‑150 (MQ=60)
                                      GACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTG                                                                                                         >  SRR19973080.3536727/1‑150 (MQ=60)
                                        CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973080.1722384/150‑1 (MQ=60)
                                        CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973080.1721668/150‑1 (MQ=60)
                                         ACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAG                                                                                                      >  SRR19973080.1508849/1‑150 (MQ=60)
                                           TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                    >  SRR19973080.4449744/1‑150 (MQ=60)
                                           TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                    >  SRR19973080.4449585/1‑150 (MQ=60)
                                            ATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATT                                                                                                   >  SRR19973080.1174686/1‑150 (MQ=60)
                                            ATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATT                                                                                                   >  SRR19973080.230675/1‑150 (MQ=60)
                                                  TTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTC                                                                                             <  SRR19973080.3127281/150‑1 (MQ=60)
                                                  TTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTC                                                                                             <  SRR19973080.4522108/150‑1 (MQ=60)
                                                  TTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTC                                                                                             <  SRR19973080.4521222/150‑1 (MQ=60)
                                                       AAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGC                                                                                        >  SRR19973080.705738/1‑150 (MQ=60)
                                                       AAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGC                                                                                        >  SRR19973080.704135/1‑150 (MQ=60)
                                                             AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCT                                                                                  >  SRR19973080.3899960/1‑150 (MQ=60)
                                                              ATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTT                                                                                 >  SRR19973080.1714569/1‑150 (MQ=60)
                                                               TGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTC                                                                                >  SRR19973080.1733182/1‑150 (MQ=60)
                                                               TGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTC                                                                                <  SRR19973080.2254556/150‑1 (MQ=60)
                                                                          CCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCG                                                                     >  SRR19973080.1097149/1‑150 (MQ=60)
                                                                           CATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGC                                                                    >  SRR19973080.3738880/1‑150 (MQ=60)
                                                                           CATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGC                                                                    >  SRR19973080.3739840/1‑150 (MQ=60)
                                                                           CATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGC                                                                    >  SRR19973080.3739029/1‑150 (MQ=60)
                                                                            ATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCA                                                                   <  SRR19973080.1621109/150‑1 (MQ=60)
                                                                               CTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCATTGCAATGCTTCGCAATATGGCGCAAAA                                                                >  SRR19973080.324661/1‑150 (MQ=60)
                                                                                TTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAT                                                               <  SRR19973080.3544427/150‑1 (MQ=60)
                                                                                TTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAT                                                               <  SRR19973080.3544428/150‑1 (MQ=60)
                                                                                 TTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATG                                                              >  SRR19973080.1351664/1‑150 (MQ=60)
                                                                                 TTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATG                                                              <  SRR19973080.1813244/150‑1 (MQ=60)
                                                                                   GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                            >  SRR19973080.879833/1‑150 (MQ=60)
                                                                                   GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                            >  SRR19973080.3134051/1‑150 (MQ=60)
                                                                                    GATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACC                                                           <  SRR19973080.4350959/150‑1 (MQ=60)
                                                                                     ATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCA                                                          >  SRR19973080.2474806/1‑150 (MQ=60)
                                                                                     ATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCA                                                          >  SRR19973080.2522416/1‑150 (MQ=60)
                                                                                     ATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACAA                                                          >  SRR19973080.1429280/1‑150 (MQ=60)
                                                                                      TAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAA                                                         >  SRR19973080.1756208/1‑150 (MQ=60)
                                                                                      TAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAA                                                         >  SRR19973080.1756688/1‑150 (MQ=60)
                                                                                        AGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACA                                                       >  SRR19973080.1142042/1‑150 (MQ=60)
                                                                                         GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      >  SRR19973080.609580/1‑150 (MQ=60)
                                                                                         GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      >  SRR19973080.4281443/1‑150 (MQ=60)
                                                                                         GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      <  SRR19973080.2469849/150‑1 (MQ=60)
                                                                                         GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      <  SRR19973080.2471978/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     <  SRR19973080.3671863/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     <  SRR19973080.2518120/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     <  SRR19973080.3751501/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     <  SRR19973080.3671870/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     <  SRR19973080.3671937/150‑1 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     >  SRR19973080.2823137/1‑150 (MQ=60)
                                                                                          GAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGC                                                     >  SRR19973080.2823487/1‑150 (MQ=60)
                                                                                           AAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCG                                                    <  SRR19973080.4412203/150‑1 (MQ=60)
                                                                                           AAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCG                                                    >  SRR19973080.792885/1‑150 (MQ=60)
                                                                                           AAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCG                                                    >  SRR19973080.790962/1‑150 (MQ=60)
                                                                                             ATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGT                                                  >  SRR19973080.1042805/1‑150 (MQ=60)
                                                                                             ATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGT                                                  >  SRR19973080.2486490/1‑150 (MQ=60)
                                                                                              TACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTT                                                 <  SRR19973080.2415770/150‑1 (MQ=60)
                                                                                              TACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTT                                                 <  SRR19973080.1529236/150‑1 (MQ=60)
                                                                                                 AGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAT                                              >  SRR19973080.3712488/1‑150 (MQ=60)
                                                                                                 AGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAT                                              <  SRR19973080.4018183/150‑1 (MQ=60)
                                                                                                  GACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACAGTGTGCGCAGCTATAAGCTTTGGAGCTTATCGTCACTGCAATGCGTCGCAATCTGGCGCAAAATGACCAACAGCGGTTGATT                                             >  SRR19973080.4363049/1‑150 (MQ=60)
                                                                                                     ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGAT                                          <  SRR19973080.2356198/150‑1 (MQ=60)
                                                                                                     ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGAT                                          >  SRR19973080.2976166/1‑150 (MQ=60)
                                                                                                         AAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG                                      <  SRR19973080.3201411/150‑1 (MQ=60)
                                                                                                         AAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG                                      >  SRR19973080.2614463/1‑150 (MQ=60)
                                                                                                         AAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG                                      <  SRR19973080.3200063/150‑1 (MQ=60)
                                                                                                         AAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGG                                      <  SRR19973080.3016278/150‑1 (MQ=60)
                                                                                                          AAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGT                                     <  SRR19973080.3495790/150‑1 (MQ=60)
                                                                                                          AAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGT                                     <  SRR19973080.246295/150‑1 (MQ=60)
                                                                                                           AATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTA                                    >  SRR19973080.392154/1‑150 (MQ=60)
                                                                                                           AATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTA                                    >  SRR19973080.1804851/1‑150 (MQ=60)
                                                                                                               TCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG                                >  SRR19973080.2750071/1‑150 (MQ=60)
                                                                                                                  TTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG                             >  SRR19973080.2782506/1‑150 (MQ=60)
                                                                                                                   TGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGC                            <  SRR19973080.2066549/150‑1 (MQ=60)
                                                                                                                      TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         >  SRR19973080.4494079/1‑150 (MQ=60)
                                                                                                                      TGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         >  SRR19973080.906160/1‑150 (MQ=60)
                                                                                                                       GAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTG                        >  SRR19973080.3520266/1‑150 (MQ=60)
                                                                                                                        AGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGT                       >  SRR19973080.684845/1‑150 (MQ=60)
                                                                                                                          TTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAC                     >  SRR19973080.3257591/1‑150 (MQ=60)
                                                                                                                           TGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACG                    <  SRR19973080.1551881/150‑1 (MQ=60)
                                                                                                                            GTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGA                   >  SRR19973080.3936633/1‑150 (MQ=60)
                                                                                                                            GTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGA                   >  SRR19973080.151414/1‑150 (MQ=60)
                                                                                                                            GTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGA                   >  SRR19973080.151317/1‑150 (MQ=60)
                                                                                                                             TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAG                  >  SRR19973080.234373/1‑150 (MQ=60)
                                                                                                                                  TAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAA             >  SRR19973080.4485189/1‑150 (MQ=60)
                                                                                                                                   AAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAG            <  SRR19973080.1726447/150‑1 (MQ=60)
                                                                                                                                             AAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  <  SRR19973080.1808091/150‑1 (MQ=60)
                                                                                                                                              |                                                                                                                                                    
CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396741‑397031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑