Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 396,883 A→G 100% Q14Q (CAA→CAG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273396,8830AG95.4% 883.0 / 18.0 239Q14Q (CAA→CAG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base A (5/6);  new base G (115/113);  total (120/119)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC  >  CP009273/396738‑397029
                                                                                                                                                 |                                                                                                                                                  
ttGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaag                                                                                                                                                <  2:4054098/150‑1 (MQ=255)
    gcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaaga                                                                                                                                            <  1:3058565/150‑1 (MQ=255)
       aaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAagaagaaga                                                                                                                                         <  1:3116150/150‑1 (MQ=255)
       aaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAagaagaaga                                                                                                                                         <  1:2538731/150‑1 (MQ=255)
        aGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAagag                                                                                                                                        <  1:857751/150‑1 (MQ=255)
          tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                      <  2:473477/150‑1 (MQ=255)
          tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTc                                                                                                                                      <  1:3397293/150‑1 (MQ=255)
          tATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTc                                                                                                                                      >  1:1001525/1‑150 (MQ=255)
           aTTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGACGAAGAGTCg                                                                                                                                     >  2:1170252/1‑150 (MQ=255)
            tttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGa                                                                                                                                    >  1:1801817/1‑150 (MQ=255)
              tCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGaaa                                                                                                                                  <  1:286755/150‑1 (MQ=255)
               ccTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAg                                                                                                                                 <  2:3167801/150‑1 (MQ=255)
               ccTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAg                                                                                                                                 <  2:930700/150‑1 (MQ=255)
                cTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGa                                                                                                                                <  1:1252701/150‑1 (MQ=255)
                cTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGa                                                                                                                                <  1:1253068/150‑1 (MQ=255)
                   tGTCATAAAAATTATACTTCCAGACACTTTGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                             <  2:160536/150‑1 (MQ=255)
                   tGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                             >  2:2626210/1‑150 (MQ=255)
                   tGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                             >  1:986437/1‑150 (MQ=255)
                   tGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                             >  1:403556/1‑150 (MQ=255)
                    gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                            >  2:1404846/1‑150 (MQ=255)
                    gTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtg                                                                                                                            >  2:1804047/1‑150 (MQ=255)
                      cATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACtgtg                                                                                                                          >  1:3337321/1‑150 (MQ=255)
                          aaaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcg                                                                                                                      >  2:2075176/1‑150 (MQ=255)
                           aaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                                                     >  1:4291226/1‑150 (MQ=255)
                           aaaTAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTgcgc                                                                                                                     >  1:1963732/1‑150 (MQ=255)
                            aataatACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCa                                                                                                                    >  1:1531188/1‑150 (MQ=255)
                               aataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGt                                                                                                                 >  2:4277788/1‑150 (MQ=255)
                               aataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGt                                                                                                                 >  2:705493/1‑150 (MQ=255)
                                 taCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAg                                                                                                               <  1:4567264/150‑1 (MQ=255)
                                  aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                              >  2:3634594/1‑150 (MQ=255)
                                  aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                              >  2:388629/1‑150 (MQ=255)
                                  aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                              >  2:4437678/1‑150 (MQ=255)
                                   cTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGaa                                                                                                             >  2:3602342/1‑150 (MQ=255)
                                   cTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGaa                                                                                                             <  2:1086117/150‑1 (MQ=255)
                                   cTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGaa                                                                                                             <  2:3983561/150‑1 (MQ=255)
                                     tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  1:3477866/1‑150 (MQ=255)
                                     tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  1:4081515/1‑150 (MQ=255)
                                     tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  2:3429721/1‑150 (MQ=255)
                                     tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  1:3022946/1‑150 (MQ=255)
                                       cAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCtt                                                                                                         >  2:3851016/1‑150 (MQ=255)
                                       cAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCtt                                                                                                         <  2:2784702/150‑1 (MQ=255)
                                        aGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                        <  2:1070571/150‑1 (MQ=255)
                                        aGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCttt                                                                                                        >  2:1731204/1‑150 (MQ=255)
                                           cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                     >  1:2985902/1‑150 (MQ=255)
                                            acTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgag                                                                                                    >  1:2626745/1‑150 (MQ=255)
                                              tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                  <  2:3329145/150‑1 (MQ=255)
                                              tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                  >  1:4183073/1‑150 (MQ=255)
                                              tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                  >  1:398378/1‑150 (MQ=255)
                                                tGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTa                                                                                                <  1:4361041/150‑1 (MQ=255)
                                                  aaGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATc                                                                                              >  1:1867230/1‑150 (MQ=255)
                                                     ttGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTc                                                                                           >  2:3106941/1‑150 (MQ=255)
                                                      tgtgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                                                          >  2:1114638/1‑150 (MQ=255)
                                                        tgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACt                                                                                        >  1:824693/1‑150 (MQ=255)
                                                               tAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGc                                                                                 <  2:3933453/150‑1 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                                <  1:737991/150‑1 (MQ=255)
                                                                aaTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCt                                                                                >  1:2393636/1‑150 (MQ=255)
                                                                   gTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCg                                                                             <  1:226484/150‑1 (MQ=255)
                                                                    ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGc                                                                            >  1:217324/1‑150 (MQ=255)
                                                                     tAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                           <  2:4147940/150‑1 (MQ=255)
                                                                     tAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                           >  1:4457507/1‑150 (MQ=255)
                                                                       aCTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAat                                                                         <  1:3806505/150‑1 (MQ=255)
                                                                         ttCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAatat                                                                       <  2:636615/150‑1 (MQ=255)
                                                                            tcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                                                    >  1:2680393/1‑150 (MQ=255)
                                                                            tcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                                                    >  2:3298611/1‑150 (MQ=255)
                                                                                  cTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaaa                                                              <  2:1337580/150‑1 (MQ=255)
                                                                                   tttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAt                                                             >  1:2760909/1‑150 (MQ=255)
                                                                                   tttGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAt                                                             <  1:2113478/150‑1 (MQ=255)
                                                                                      ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                          <  1:581062/150‑1 (MQ=255)
                                                                                      ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                          <  1:4242366/150‑1 (MQ=255)
                                                                                      ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                          >  1:2544590/1‑150 (MQ=255)
                                                                                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                         >  2:4281987/1‑150 (MQ=255)
                                                                                       gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                         >  1:1409498/1‑150 (MQ=255)
                                                                                        aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCACAGAAAGAAGAAGAGTCGAAATAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCa                                                        <  2:1696684/150‑1 (MQ=255)
                                                                                          aaGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAc                                                      >  1:1822553/1‑150 (MQ=255)
                                                                                             gAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGc                                                   <  2:4146183/150‑1 (MQ=255)
                                                                                              aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                                  >  2:4081462/1‑150 (MQ=255)
                                                                                                   cAGACATGAAAAAACTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGAGTGCGCAGGTAGAAGCTTTGGAGATTATCGACACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga                                             >  1:4239177/1‑150 (MQ=255)
                                                                                                     gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt                                           <  2:3535267/150‑1 (MQ=255)
                                                                                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          >  2:2910462/1‑150 (MQ=255)
                                                                                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          <  2:4088804/150‑1 (MQ=255)
                                                                                                      aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          >  2:1321915/1‑150 (MQ=255)
                                                                                                        aTGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                        <  1:3378284/150‑1 (MQ=255)
                                                                                                        aTGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTtgattgat                                        <  2:225644/150‑1 (MQ=255)
                                                                                                         tGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATc                                       >  1:2853822/1‑150 (MQ=255)
                                                                                                         tGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATc                                       <  2:2407282/150‑1 (MQ=255)
                                                                                                                  tcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                              >  1:2365227/1‑150 (MQ=255)
                                                                                                                   cATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggg                             <  1:712593/150‑1 (MQ=255)
                                                                                                                     ttGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggg                           <  1:2961715/150‑1 (MQ=255)
                                                                                                                     ttGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggg                           <  1:3469754/150‑1 (MQ=255)
                                                                                                                        cTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgcgc                        >  2:821855/1‑150 (MQ=255)
                                                                                                                          gAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTg                      <  2:86798/150‑1 (MQ=255)
                                                                                                                                ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                >  2:39158/1‑150 (MQ=255)
                                                                                                                                  aTTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGt              <  2:4231437/150‑1 (MQ=255)
                                                                                                                                     tAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa           <  2:1481912/150‑1 (MQ=255)
                                                                                                                                      aaGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg          >  1:1925150/1‑150 (MQ=255)
                                                                                                                                         cTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGccc       <  2:2030773/150‑1 (MQ=255)
                                                                                                                                          ttGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg      >  1:685535/1‑150 (MQ=255)
                                                                                                                                          ttGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCg      <  1:2823292/150‑1 (MQ=255)
                                                                                                                                              ccAGAATGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCTAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGc  <  2:3681852/150‑1 (MQ=255)
                                                                                                                                              ccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGc  <  2:370926/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC  >  CP009273/396738‑397029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCC  >  CP009273/396740‑397030
                                                                                                                                               |                                                                                                                                                   
GCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAA                                                                                                                                               <  SRR19973073.254110/150‑1 (MQ=60)
  GCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGA                                                                                                                                             <  SRR19973073.3064726/150‑1 (MQ=60)
   CAAAGTATTTCCTTTGTCATAAAAATAATANTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAA                                                                                                                                            >  SRR19973073.4285593/1‑150 (MQ=60)
     AAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGA                                                                                                                                          <  SRR19973073.2543971/150‑1 (MQ=60)
     AAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGA                                                                                                                                          <  SRR19973073.3122412/150‑1 (MQ=60)
      AGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAG                                                                                                                                         <  SRR19973073.859526/150‑1 (MQ=60)
       GTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAATTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGT                                                                                                                                        <  SRR19973073.2853265/150‑1 (MQ=60)
        TATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTC                                                                                                                                       <  SRR19973073.3404087/150‑1 (MQ=60)
        TATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTC                                                                                                                                       <  SRR19973073.3404106/150‑1 (MQ=60)
        TATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTC                                                                                                                                       >  SRR19973073.1003620/1‑150 (MQ=60)
         ATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCG                                                                                                                                      >  SRR19973073.2988679/1‑150 (MQ=60)
          TTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGA                                                                                                                                     <  SRR19973073.3024385/150‑1 (MQ=60)
          TTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGA                                                                                                                                     >  SRR19973073.1805533/1‑150 (MQ=60)
          TTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGA                                                                                                                                     >  SRR19973073.1805542/1‑150 (MQ=60)
            TCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAA                                                                                                                                   <  SRR19973073.2522100/150‑1 (MQ=60)
            TCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAA                                                                                                                                   <  SRR19973073.287375/150‑1 (MQ=60)
              CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                 <  SRR19973073.1255690/150‑1 (MQ=60)
              CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                 <  SRR19973073.1255689/150‑1 (MQ=60)
              CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                 <  SRR19973073.1255321/150‑1 (MQ=60)
                 TGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACT                                                                                                                              >  SRR19973073.404344/1‑150 (MQ=60)
                 TGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACT                                                                                                                              >  SRR19973073.404348/1‑150 (MQ=60)
                 TGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACT                                                                                                                              >  SRR19973073.404361/1‑150 (MQ=60)
                 TGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACT                                                                                                                              >  SRR19973073.988492/1‑150 (MQ=60)
                   TCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGT                                                                                                                            <  SRR19973073.979097/150‑1 (MQ=60)
                   TCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGT                                                                                                                            >  SRR19973073.2650406/1‑150 (MQ=60)
                    CATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTG                                                                                                                           >  SRR19973073.3344068/1‑150 (MQ=60)
                        AAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCG                                                                                                                       <  SRR19973073.3192481/150‑1 (MQ=60)
                         AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                      >  SRR19973073.4299764/1‑150 (MQ=60)
                         AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                      >  SRR19973073.725914/1‑150 (MQ=60)
                         AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                      >  SRR19973073.3044529/1‑150 (MQ=60)
                         AAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGC                                                                                                                      >  SRR19973073.1967783/1‑150 (MQ=60)
                          AATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCA                                                                                                                     >  SRR19973073.1534237/1‑150 (MQ=60)
                          AATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCA                                                                                                                     >  SRR19973073.1534331/1‑150 (MQ=60)
                           ATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAG                                                                                                                    >  SRR19973073.275010/1‑150 (MQ=60)
                           ATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAG                                                                                                                    >  SRR19973073.423112/1‑150 (MQ=60)
                              ATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTA                                                                                                                 >  SRR19973073.1941581/1‑150 (MQ=60)
                               TACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAG                                                                                                                <  SRR19973073.4576403/150‑1 (MQ=60)
                                  TTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAG                                                                                                             <  SRR19973073.4368643/150‑1 (MQ=60)
                                   TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                            >  SRR19973073.3484785/1‑150 (MQ=60)
                                   TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                            >  SRR19973073.4089689/1‑150 (MQ=60)
                                   TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                            >  SRR19973073.3029065/1‑150 (MQ=60)
                                   TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                            >  SRR19973073.3484649/1‑150 (MQ=60)
                                        ACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGG                                                                                                       <  SRR19973073.4609193/150‑1 (MQ=60)
                                        ACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGG                                                                                                       >  SRR19973073.3763235/1‑150 (MQ=60)
                                         CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                      >  SRR19973073.2991974/1‑150 (MQ=60)
                                          ACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAG                                                                                                     >  SRR19973073.2632151/1‑150 (MQ=60)
                                          ACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAG                                                                                                     >  SRR19973073.2997575/1‑150 (MQ=60)
                                          ACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAG                                                                                                     >  SRR19973073.2632144/1‑150 (MQ=60)
                                            TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                   >  SRR19973073.399171/1‑150 (MQ=60)
                                            TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                   >  SRR19973073.4191390/1‑150 (MQ=60)
                                              TGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTA                                                                                                 <  SRR19973073.4369778/150‑1 (MQ=60)
                                                AAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATC                                                                                               <  SRR19973073.74192/150‑1 (MQ=60)
                                                AAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATC                                                                                               >  SRR19973073.1871059/1‑150 (MQ=60)
                                                AAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATC                                                                                               <  SRR19973073.2113793/150‑1 (MQ=60)
                                                 AGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCG                                                                                              <  SRR19973073.1913456/150‑1 (MQ=60)
                                                    TGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCA                                                                                           >  SRR19973073.2333290/1‑150 (MQ=60)
                                                      TGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACT                                                                                         >  SRR19973073.826506/1‑150 (MQ=60)
                                                      TGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACT                                                                                         >  SRR19973073.826379/1‑150 (MQ=60)
                                                         AACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCA                                                                                      <  SRR19973073.2860239/150‑1 (MQ=60)
                                                             TAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGC                                                                                  >  SRR19973073.1676926/1‑150 (MQ=60)
                                                             TAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGC                                                                                  >  SRR19973073.3305038/1‑150 (MQ=60)
                                                              AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCT                                                                                 <  SRR19973073.739495/150‑1 (MQ=60)
                                                              AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCT                                                                                 >  SRR19973073.2398549/1‑150 (MQ=60)
                                                              AATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCT                                                                                 <  SRR19973073.739501/150‑1 (MQ=60)
                                                                 GTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCG                                                                              <  SRR19973073.227005/150‑1 (MQ=60)
                                                                  TTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGC                                                                             >  SRR19973073.217833/1‑150 (MQ=60)
                                                                   TAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCA                                                                            >  SRR19973073.4466462/1‑150 (MQ=60)
                                                                    AACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAA                                                                           >  SRR19973073.596984/1‑150 (MQ=60)
                                                                    AACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAA                                                                           >  SRR19973073.2914435/1‑150 (MQ=60)
                                                                     ACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAAT                                                                          <  SRR19973073.3814099/150‑1 (MQ=60)
                                                                       TTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATAT                                                                        >  SRR19973073.1297352/1‑150 (MQ=60)
                                                                          TCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGC                                                                     >  SRR19973073.2685872/1‑150 (MQ=60)
                                                                                 TTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAT                                                              <  SRR19973073.2117914/150‑1 (MQ=60)
                                                                                 TTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAT                                                              >  SRR19973073.2766563/1‑150 (MQ=60)
                                                                                 TTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAT                                                              <  SRR19973073.2117903/150‑1 (MQ=60)
                                                                                    GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                           <  SRR19973073.4250764/150‑1 (MQ=60)
                                                                                    GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                           <  SRR19973073.582306/150‑1 (MQ=60)
                                                                                    GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                           >  SRR19973073.2549629/1‑150 (MQ=60)
                                                                                    GGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAC                                                           >  SRR19973073.2549845/1‑150 (MQ=60)
                                                                                     GATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACC                                                          >  SRR19973073.1412475/1‑150 (MQ=60)
                                                                                      ATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCA                                                         <  SRR19973073.446136/150‑1 (MQ=60)
                                                                                       TAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAA                                                        >  SRR19973073.4533700/1‑150 (MQ=60)
                                                                                        AAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAC                                                       <  SRR19973073.3553586/150‑1 (MQ=60)
                                                                                        AAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAC                                                       >  SRR19973073.1826320/1‑150 (MQ=60)
                                                                                              ATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGT                                                 >  SRR19973073.4118453/1‑150 (MQ=60)
                                                                                               TACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTT                                                <  SRR19973073.3468372/150‑1 (MQ=60)
                                                                                                 CAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGA                                              >  SRR19973073.4247545/1‑150 (MQ=60)
                                                                                                 CAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGA                                              >  SRR19973073.4247815/1‑150 (MQ=60)
                                                                                                 CAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGA                                              <  SRR19973073.2285097/150‑1 (MQ=60)
                                                                                                 CAGACATGAAAAAACTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGAGTGCGCAGGTAGAAGCTTTGGAGATTATCGACACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGA                                              >  SRR19973073.4247568/1‑150 (MQ=60)
                                                                                                     CATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGA                                          >  SRR19973073.1542470/1‑150 (MQ=60)
                                                                                                     CATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGA                                          >  SRR19973073.1401718/1‑150 (MQ=60)
                                                                                                      ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGAT                                         <  SRR19973073.3384991/150‑1 (MQ=60)
                                                                                                      ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGAT                                         <  SRR19973073.3385085/150‑1 (MQ=60)
                                                                                                      ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGAT                                         <  SRR19973073.3403870/150‑1 (MQ=60)
                                                                                                      ATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAGTGAT                                         >  SRR19973073.597568/1‑150 (MQ=60)
                                                                                                       TGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATC                                        <  SRR19973073.2002678/150‑1 (MQ=60)
                                                                                                       TGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATC                                        >  SRR19973073.2859669/1‑150 (MQ=60)
                                                                                                        GAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCA                                       <  SRR19973073.4044734/150‑1 (MQ=60)
                                                                                                               CTCATTGCTGAGTTGTTATTTATGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                <  SRR19973073.1161535/150‑1 (MQ=60)
                                                                                                               CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                <  SRR19973073.4277436/150‑1 (MQ=60)
                                                                                                                TCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG                               >  SRR19973073.2370064/1‑150 (MQ=60)
                                                                                                                 CATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG                              <  SRR19973073.714063/150‑1 (MQ=60)
                                                                                                                  ATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGG                             >  SRR19973073.3979088/1‑150 (MQ=60)
                                                                                                                   TTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG                            <  SRR19973073.2967748/150‑1 (MQ=60)
                                                                                                                   TTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG                            <  SRR19973073.3476650/150‑1 (MQ=60)
                                                                                                                       TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                        >  SRR19973073.4476795/1‑150 (MQ=60)
                                                                                                                             GTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGA                  <  SRR19973073.2961598/150‑1 (MQ=60)
                                                                                                                                ATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGT               <  SRR19973073.920068/150‑1 (MQ=60)
                                                                                                                                ATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGT               <  SRR19973073.2220224/150‑1 (MQ=60)
                                                                                                                                 TTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA              <  SRR19973073.3091349/150‑1 (MQ=60)
                                                                                                                                 TTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA              <  SRR19973073.1838067/150‑1 (MQ=60)
                                                                                                                                   TAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAA            <  SRR19973073.2378155/150‑1 (MQ=60)
                                                                                                                                    AAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAG           >  SRR19973073.1929064/1‑150 (MQ=60)
                                                                                                                                    AAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAG           >  SRR19973073.713775/1‑150 (MQ=60)
                                                                                                                                    AAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAG           >  SRR19973073.1929049/1‑150 (MQ=60)
                                                                                                                                        TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG       <  SRR19973073.2829022/150‑1 (MQ=60)
                                                                                                                                        TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG       <  SRR19973073.2829019/150‑1 (MQ=60)
                                                                                                                                        TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG       >  SRR19973073.2569271/1‑150 (MQ=60)
                                                                                                                                        TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG       <  SRR19973073.2829083/150‑1 (MQ=60)
                                                                                                                                        TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG       >  SRR19973073.686961/1‑150 (MQ=60)
                                                                                                                                             CAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCC  >  SRR19973073.786529/1‑150 (MQ=60)
                                                                                                                                               |                                                                                                                                                   
GCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCC  >  CP009273/396740‑397030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑