Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 396,883 A→G 100% Q14Q (CAA→CAG)  iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273396,8830AG99.0% 792.8 / ‑3.0 206Q14Q (CAA→CAG) iraPanti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (116/88);  total (116/90)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC  >  CP009273/396737‑397029
                                                                                                                                                  |                                                                                                                                                  
 ttGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATATTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaag                                                                                                                                                <  1:3985519/150‑1 (MQ=255)
 ttGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaag                                                                                                                                                <  2:754553/150‑1 (MQ=255)
    cgcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaag                                                                                                                                             <  2:3356372/150‑1 (MQ=255)
    cgcAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaag                                                                                                                                             >  1:1505787/1‑150 (MQ=255)
      cAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAaagaagaa                                                                                                                                           <  1:537754/150‑1 (MQ=255)
        aaGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAagaagaaga                                                                                                                                         <  1:3244316/150‑1 (MQ=255)
         aGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAagag                                                                                                                                        <  1:2361947/150‑1 (MQ=255)
          gTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGt                                                                                                                                       >  1:3177147/1‑150 (MQ=255)
             tttCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGa                                                                                                                                    <  2:3855378/150‑1 (MQ=255)
                ccTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAg                                                                                                                                 >  1:4508739/1‑150 (MQ=255)
                 cTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGa                                                                                                                                >  2:1087037/1‑150 (MQ=255)
                   ttGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAAc                                                                                                                              <  1:2749960/150‑1 (MQ=255)
                    tGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACt                                                                                                                             >  2:1945526/1‑150 (MQ=255)
                     gTCATAAAAATAATACTTCCAGACAATATGAAGATGTGAAACATCATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATAGCTGAGTTGTTATTTAATCTTGCCCAGAAAGAAGAAGATTCGAAAGAACtg                                                                                                                            >  1:942027/1‑150 (MQ=255)
                             aataatACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCa                                                                                                                    <  2:1563920/150‑1 (MQ=255)
                               taataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAgg                                                                                                                  <  1:1527693/150‑1 (MQ=255)
                                 ataCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTa                                                                                                                >  2:3046311/1‑150 (MQ=255)
                                  taCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAg                                                                                                               <  1:3321556/150‑1 (MQ=255)
                                  taCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAg                                                                                                               >  2:1579762/1‑150 (MQ=255)
                                   aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                              >  2:1169249/1‑150 (MQ=255)
                                   aCTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGa                                                                                                              <  1:1509491/150‑1 (MQ=255)
                                    cTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGaa                                                                                                             <  1:296589/150‑1 (MQ=255)
                                     ttCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGGACTGTGTGCGCAGGTAGAAg                                                                                                            >  1:2498862/1‑150 (MQ=255)
                                     ttCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAg                                                                                                            <  2:4349016/150‑1 (MQ=255)
                                      tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  1:4903057/1‑150 (MQ=255)
                                      tCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGc                                                                                                           >  2:461109/1‑150 (MQ=255)
                                       ccAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCt                                                                                                          <  2:3991308/150‑1 (MQ=255)
                                          gACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                                                       <  2:1706181/150‑1 (MQ=255)
                                          gACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTg                                                                                                       <  1:46869/150‑1 (MQ=255)
                                           acacTATGAAGTTGTGAAACATACTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAANAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                                                      <  1:2678133/150‑1 (MQ=255)
                                           acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                                                      <  1:3384763/150‑1 (MQ=255)
                                           acacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTgg                                                                                                      <  1:4582027/150‑1 (MQ=255)
                                            cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                     <  1:961572/150‑1 (MQ=255)
                                            cacTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGga                                                                                                     <  1:4972082/150‑1 (MQ=255)
                                             acTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgag                                                                                                    >  2:2098866/1‑150 (MQ=255)
                                              cTATGAAGTTGTGAAACATAATGTTAACGTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGgaga                                                                                                   <  1:4240597/150‑1 (MQ=255)
                                               tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                  <  1:2960715/150‑1 (MQ=255)
                                               tATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAt                                                                                                  >  2:4893591/1‑150 (MQ=255)
                                                 tGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTa                                                                                                <  1:2434878/150‑1 (MQ=255)
                                                   aaGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATc                                                                                              >  1:2159870/1‑150 (MQ=255)
                                                      ttGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTc                                                                                           <  2:5156535/150‑1 (MQ=255)
                                                       tgtgAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCa                                                                                          <  1:1196421/150‑1 (MQ=255)
                                                              cATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAAt                                                                                   <  2:3755987/150‑1 (MQ=255)
                                                                tAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGc                                                                                 <  2:1742283/150‑1 (MQ=255)
                                                                     ttAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGc                                                                            >  1:1636274/1‑150 (MQ=255)
                                                                      tAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCa                                                                           >  1:4963018/1‑150 (MQ=255)
                                                                          ttCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAatat                                                                       <  1:3629093/150‑1 (MQ=255)
                                                                           tctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                                                      >  2:1123561/1‑150 (MQ=255)
                                                                           tctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATg                                                                      <  2:2444512/150‑1 (MQ=255)
                                                                            ctcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATgg                                                                     >  1:678725/1‑150 (MQ=255)
                                                                             tcCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgc                                                                    <  2:230581/150‑1 (MQ=255)
                                                                               cATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGgcgc                                                                  >  1:1604922/1‑150 (MQ=255)
                                                                                aTACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCa                                                                 >  2:2939913/1‑150 (MQ=255)
                                                                                 tACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaa                                                                >  1:2247929/1‑150 (MQ=255)
                                                                                  aCTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaa                                                               >  1:1313172/1‑150 (MQ=255)
                                                                                   cTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCaaaa                                                              <  1:263247/150‑1 (MQ=255)
                                                                                       ggATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAc                                                          >  2:4313531/1‑150 (MQ=255)
                                                                                        gATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGAcc                                                         >  1:3242836/1‑150 (MQ=255)
                                                                                         aTAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCa                                                        <  1:525979/150‑1 (MQ=255)
                                                                                             ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                    <  1:3491203/150‑1 (MQ=255)
                                                                                             ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                    <  2:436583/150‑1 (MQ=255)
                                                                                             ggAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAACAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAg                                                    <  1:4529649/150‑1 (MQ=255)
                                                                                               aaaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                                  >  1:2717200/1‑150 (MQ=255)
                                                                                               aaaTACAGACATGAAAAATCTCAGTGCTGAGTTGTTATTTAAGCGTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCg                                                  <  2:3553543/150‑1 (MQ=255)
                                                                                                aaTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCgg                                                 >  2:3827386/1‑150 (MQ=255)
                                                                                                 aTACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGt                                                <  1:1879113/150‑1 (MQ=255)
                                                                                                    cAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttga                                             >  2:3709671/1‑150 (MQ=255)
                                                                                                     aGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgat                                            >  1:3926587/1‑150 (MQ=255)
                                                                                                      gACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgatt                                           <  2:2802908/150‑1 (MQ=255)
                                                                                                       aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          <  2:3395616/150‑1 (MQ=255)
                                                                                                       aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          >  1:1863208/1‑150 (MQ=255)
                                                                                                       aCATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattg                                          >  1:1004623/1‑150 (MQ=255)
                                                                                                        cATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGttgattga                                         <  1:4313531/150‑1 (MQ=255)
                                                                                                          tGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATc                                       >  2:634636/1‑150 (MQ=255)
                                                                                                            aaaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAg                                     >  1:3286236/1‑150 (MQ=255)
                                                                                                             aaaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAgg                                    <  2:1933658/150‑1 (MQ=255)
                                                                                                              aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGt                                   <  1:857330/150‑1 (MQ=255)
                                                                                                              aaaTCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGt                                   >  2:1481574/1‑150 (MQ=255)
                                                                                                               aaTCTCATTGCTGAGTCGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTa                                  >  2:3340033/1‑150 (MQ=255)
                                                                                                                  ctcATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagag                               <  1:1091892/150‑1 (MQ=255)
                                                                                                                        gCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGgcg                         >  2:2712807/1‑150 (MQ=255)
                                                                                                                          tGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                       >  2:2354648/1‑150 (MQ=255)
                                                                                                                          tGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                       <  1:3745701/150‑1 (MQ=255)
                                                                                                                          tGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                       <  1:4191639/150‑1 (MQ=255)
                                                                                                                          tGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                       <  1:632465/150‑1 (MQ=255)
                                                                                                                          tGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCt                       <  2:2078557/150‑1 (MQ=255)
                                                                                                                              ttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAc                   >  2:3672163/1‑150 (MQ=255)
                                                                                                                              ttgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAc                   >  1:416113/1‑150 (MQ=255)
                                                                                                                               tgttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACg                  >  1:4454766/1‑150 (MQ=255)
                                                                                                                                 ttATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAg                <  2:2502151/150‑1 (MQ=255)
                                                                                                                                    tttAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACGTTGTGCGCAGGTAGAAGCTGTGGAGATTATCGTCACTGCAATGCTTCGAAATCTGGCGCAAAATGACCAACAGCGGGTGATTGATCAGGGAGAGGGGGCGCTGTAAGAGGTa             >  2:3068483/1‑150 (MQ=255)
                                                                                                                                      tAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa           >  1:1333610/1‑150 (MQ=255)
                                                                                                                                      tAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTaaa           <  1:17331/150‑1 (MQ=255)
                                                                                                                                       aaGCTGGCCCAGAACGAAGAAGAGTCGAAAGAACTGTGGGCGCAGGTAGAAGCGTTGGAGATTAGCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAg          <  2:2626199/150‑1 (MQ=255)
                                                                                                                                          cTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGccc       >  1:932619/1‑150 (MQ=255)
                                                                                                                                            tGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGa     <  2:1016703/150‑1 (MQ=255)
                                                                                                                                              cccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATg   >  2:2146639/1‑150 (MQ=255)
                                                                                                                                              cccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATg   >  2:3528453/1‑150 (MQ=255)
                                                                                                                                               ccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGc  >  1:78213/1‑150 (MQ=255)
                                                                                                                                               ccAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGc  >  1:3358326/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC  >  CP009273/396737‑397029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396738‑397031
                                                                                                                                                 |                                                                                                                                                    
TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATATTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAG                                                                                                                                                  <  SRR19973063.3998292/150‑1 (MQ=60)
  GCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAA                                                                                                                                                <  SRR19973063.275387/150‑1 (MQ=60)
   CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAG                                                                                                                                               >  SRR19973063.4243809/1‑150 (MQ=60)
   CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAG                                                                                                                                               >  SRR19973063.1510396/1‑150 (MQ=60)
   CGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAG                                                                                                                                               >  SRR19973063.1510562/1‑150 (MQ=60)
     CAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAA                                                                                                                                             <  SRR19973063.539343/150‑1 (MQ=60)
      AAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGNCACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAG                                                                                                                                            >  SRR19973063.2684424/1‑150 (MQ=60)
      AAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAG                                                                                                                                            >  SRR19973063.1255862/1‑150 (MQ=60)
      AAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAG                                                                                                                                            >  SRR19973063.4091056/1‑150 (MQ=60)
       AAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGA                                                                                                                                           <  SRR19973063.3254734/150‑1 (MQ=60)
        AGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAG                                                                                                                                          <  SRR19973063.2369513/150‑1 (MQ=60)
        AGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAG                                                                                                                                          >  SRR19973063.2370113/1‑150 (MQ=60)
        AGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAG                                                                                                                                          >  SRR19973063.3731078/1‑150 (MQ=60)
         GTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGT                                                                                                                                         >  SRR19973063.3187368/1‑150 (MQ=60)
           ATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGATGAGTCG                                                                                                                                       >  SRR19973063.955423/1‑150 (MQ=60)
               CCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAG                                                                                                                                   >  SRR19973063.4523208/1‑150 (MQ=60)
                CTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGA                                                                                                                                  >  SRR19973063.984535/1‑150 (MQ=60)
                  TTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAAC                                                                                                                                <  SRR19973063.2758832/150‑1 (MQ=60)
                    GTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTG                                                                                                                              <  SRR19973063.4534573/150‑1 (MQ=60)
                    GTCATAAAAATAATACTTCCAGACAATATGAAGATGTGAAACATCATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATAGCTGAGTTGTTATTTAATCTTGCCCAGAAAGAAGAAGATTCGAAAGAACTG                                                                                                                              >  SRR19973063.944839/1‑150 (MQ=60)
                              TAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGG                                                                                                                    <  SRR19973063.1532376/150‑1 (MQ=60)
                                ATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTA                                                                                                                  <  SRR19973063.4983072/150‑1 (MQ=60)
                                 TACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAG                                                                                                                 <  SRR19973063.3332164/150‑1 (MQ=60)
                                  ACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGA                                                                                                                <  SRR19973063.1514118/150‑1 (MQ=60)
                                   CTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAA                                                                                                               <  SRR19973063.297463/150‑1 (MQ=60)
                                   CTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAA                                                                                                               >  SRR19973063.334975/1‑150 (MQ=60)
                                    TTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGGACTGTGTGCGCAGGTAGAAG                                                                                                              >  SRR19973063.2506834/1‑150 (MQ=60)
                                    TTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAG                                                                                                              >  SRR19973063.2506830/1‑150 (MQ=60)
                                     TCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGC                                                                                                             >  SRR19973063.4918888/1‑150 (MQ=60)
                                         GACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTG                                                                                                         <  SRR19973063.47036/150‑1 (MQ=60)
                                          ACACTATGAAGTTGTGAAACATACTGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAANAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGG                                                                                                        <  SRR19973063.2686745/150‑1 (MQ=60)
                                          ACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGG                                                                                                        <  SRR19973063.3395551/150‑1 (MQ=60)
                                          ACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGG                                                                                                        <  SRR19973063.4596693/150‑1 (MQ=60)
                                           CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973063.964466/150‑1 (MQ=60)
                                           CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973063.4988153/150‑1 (MQ=60)
                                           CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973063.1422382/150‑1 (MQ=60)
                                           CACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGCCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGA                                                                                                       <  SRR19973063.1653738/150‑1 (MQ=60)
                                            ACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAG                                                                                                      >  SRR19973063.1073631/1‑150 (MQ=60)
                                             CTATGAAGTTGTGAAACATAATGTTAACGTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGA                                                                                                     <  SRR19973063.4254113/150‑1 (MQ=60)
                                              TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                    <  SRR19973063.2970244/150‑1 (MQ=60)
                                              TATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGAT                                                                                                    <  SRR19973063.2970184/150‑1 (MQ=60)
                                               ATGAAGTTGTGAAACTTAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATT                                                                                                   <  SRR19973063.1637078/150‑1 (MQ=60)
                                               ATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATT                                                                                                   <  SRR19973063.4252077/150‑1 (MQ=60)
                                               ATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTAAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATT                                                                                                   >  SRR19973063.4999466/1‑150 (MQ=60)
                                                TGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTA                                                                                                  <  SRR19973063.2442659/150‑1 (MQ=60)
                                                  AAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATC                                                                                                >  SRR19973063.2166635/1‑150 (MQ=60)
                                                      TGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCA                                                                                            <  SRR19973063.1200012/150‑1 (MQ=60)
                                                             CATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAAT                                                                                     <  SRR19973063.325303/150‑1 (MQ=60)
                                                              ATAATGTTAACGTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATG                                                                                    <  SRR19973063.3185356/150‑1 (MQ=60)
                                                                    TTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGC                                                                              >  SRR19973063.2291709/1‑150 (MQ=60)
                                                                    TTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGC                                                                              >  SRR19973063.1641279/1‑150 (MQ=60)
                                                                     TAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCA                                                                             >  SRR19973063.4979062/1‑150 (MQ=60)
                                                                     TAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCA                                                                             >  SRR19973063.2226361/1‑150 (MQ=60)
                                                                     TAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCA                                                                             <  SRR19973063.3989324/150‑1 (MQ=60)
                                                                      AACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAA                                                                            >  SRR19973063.2014957/1‑150 (MQ=60)
                                                                         TTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATAT                                                                         <  SRR19973063.3640741/150‑1 (MQ=60)
                                                                           CTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAGGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGG                                                                       <  SRR19973063.1604787/150‑1 (MQ=60)
                                                                           CTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGG                                                                       <  SRR19973063.3933733/150‑1 (MQ=60)
                                                                           CTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGG                                                                       >  SRR19973063.680711/1‑150 (MQ=60)
                                                                              CATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGC                                                                    >  SRR19973063.1609837/1‑150 (MQ=60)
                                                                              CATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGC                                                                    >  SRR19973063.151475/1‑150 (MQ=60)
                                                                                TACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAA                                                                  >  SRR19973063.2255081/1‑150 (MQ=60)
                                                                                 ACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAA                                                                 >  SRR19973063.1317088/1‑150 (MQ=60)
                                                                                  CTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAA                                                                <  SRR19973063.264034/150‑1 (MQ=60)
                                                                                    TTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATG                                                              >  SRR19973063.1912905/1‑150 (MQ=60)
                                                                                     TGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGA                                                             >  SRR19973063.3447657/1‑150 (MQ=60)
                                                                                       GATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACC                                                           >  SRR19973063.3253253/1‑150 (MQ=60)
                                                                                        ATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCA                                                          <  SRR19973063.527518/150‑1 (MQ=60)
                                                                                           AGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACA                                                       >  SRR19973063.3213185/1‑150 (MQ=60)
                                                                                            GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      <  SRR19973063.3502361/150‑1 (MQ=60)
                                                                                            GGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAACAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAG                                                      <  SRR19973063.4544177/150‑1 (MQ=60)
                                                                                              AAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCG                                                    >  SRR19973063.2590278/1‑150 (MQ=60)
                                                                                              AAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCG                                                    >  SRR19973063.2725977/1‑150 (MQ=60)
                                                                                                ATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGT                                                  <  SRR19973063.1884986/150‑1 (MQ=60)
                                                                                                ATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGT                                                  <  SRR19973063.1884913/150‑1 (MQ=60)
                                                                                                 TACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTT                                                 >  SRR19973063.4568201/1‑150 (MQ=60)
                                                                                                    AGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAT                                              >  SRR19973063.3939119/1‑150 (MQ=60)
                                                                                                    AGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAT                                              >  SRR19973063.528428/1‑150 (MQ=60)
                                                                                                      ACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTG                                            >  SRR19973063.1007648/1‑150 (MQ=60)
                                                                                                      ACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTG                                            >  SRR19973063.1869031/1‑150 (MQ=60)
                                                                                                       CATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGA                                           <  SRR19973063.1741230/150‑1 (MQ=60)
                                                                                                       CATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGA                                           <  SRR19973063.4327234/150‑1 (MQ=60)
                                                                                                         TGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATC                                         >  SRR19973063.4661360/1‑150 (MQ=60)
                                                                                                           AAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAG                                       <  SRR19973063.2830120/150‑1 (MQ=60)
                                                                                                           AAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAG                                       >  SRR19973063.3296563/1‑150 (MQ=60)
                                                                                                           AAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAG                                       >  SRR19973063.3296772/1‑150 (MQ=60)
                                                                                                             AAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGT                                     <  SRR19973063.859799/150‑1 (MQ=60)
                                                                                                                TCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA                                  <  SRR19973063.443091/150‑1 (MQ=60)
                                                                                                                 CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                 >  SRR19973063.321291/1‑150 (MQ=60)
                                                                                                                 CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                 <  SRR19973063.1095209/150‑1 (MQ=60)
                                                                                                                 CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                 <  SRR19973063.632592/150‑1 (MQ=60)
                                                                                                                 CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                 >  SRR19973063.4791464/1‑150 (MQ=60)
                                                                                                                 CTCATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAG                                 <  SRR19973063.725723/150‑1 (MQ=60)
                                                                                                                   CATTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGG                               >  SRR19973063.859901/1‑150 (MQ=60)
                                                                                                                     TTGCTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG                             >  SRR19973063.4276303/1‑150 (MQ=60)
                                                                                                                        CTGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGC                          >  SRR19973063.4186049/1‑150 (MQ=60)
                                                                                                                         TTAGTTGTTATTTAATCTTGCCCAGAAAGAAGAAGACTCGAAACAACTTTGTGCGCATGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAAAGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         <  SRR19973063.634254/150‑1 (MQ=60)
                                                                                                                         TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         <  SRR19973063.634322/150‑1 (MQ=60)
                                                                                                                         TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         <  SRR19973063.634493/150‑1 (MQ=60)
                                                                                                                         TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         <  SRR19973063.3757670/150‑1 (MQ=60)
                                                                                                                         TGAGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCT                         <  SRR19973063.4205024/150‑1 (MQ=60)
                                                                                                                           AGTTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCCCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGT                       <  SRR19973063.3536406/150‑1 (MQ=60)
                                                                                                                             TTGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAC                     >  SRR19973063.417281/1‑150 (MQ=60)
                                                                                                                              TGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACG                    >  SRR19973063.4469066/1‑150 (MQ=60)
                                                                                                                              TGTTATTTAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACG                    >  SRR19973063.4469100/1‑150 (MQ=60)
                                                                                                                                     TAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAA             <  SRR19973063.17397/150‑1 (MQ=60)
                                                                                                                                     TAAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAA             >  SRR19973063.1337609/1‑150 (MQ=60)
                                                                                                                                      AAGCTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAG            >  SRR19973063.3320633/1‑150 (MQ=60)
                                                                                                                                         CTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC         >  SRR19973063.825789/1‑150 (MQ=60)
                                                                                                                                         CTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC         >  SRR19973063.935402/1‑150 (MQ=60)
                                                                                                                                         CTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC         >  SRR19973063.935500/1‑150 (MQ=60)
                                                                                                                                         CTTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC         >  SRR19973063.1318714/1‑150 (MQ=60)
                                                                                                                                          TTGCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCG        >  SRR19973063.4957827/1‑150 (MQ=60)
                                                                                                                                            GCCCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGAT      >  SRR19973063.115171/1‑150 (MQ=60)
                                                                                                                                              CCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC    >  SRR19973063.3369075/1‑150 (MQ=60)
                                                                                                                                              CCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC    >  SRR19973063.3369015/1‑150 (MQ=60)
                                                                                                                                              CCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC    <  SRR19973063.2709159/150‑1 (MQ=60)
                                                                                                                                              CCAGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGC    >  SRR19973063.78484/1‑150 (MQ=60)
                                                                                                                                                AGAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  SRR19973063.4565258/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
TTGCGCAAAGTATTTCCTTTGTCATAAAAATAATACTTCCAGACACTATGAAGTTGTGAAACATAATGTTAACTTCTCCATACTTTGGATAAGGAAATACAGACATGAAAAATCTCATTGCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCA  >  CP009273/396738‑397031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑