Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,007,547 C→A 5.2% R280S (CGC→AGC)  fliF → flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,007,5470CA 5.2% 1034.9 / 24.0 268R280S (CGC→AGC) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base C (130/124);  new base A (10/4);  total (140/128)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

CTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCG  >  CP009273/2007398‑2007696
                                                                                                                                                     |                                                                                                                                                     
 tCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCg                                                                                                                                                      <  2:3595715/150‑1 (MQ=255)
   aGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGcc                                                                                                                                                    <  2:2920352/150‑1 (MQ=255)
      tGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTa                                                                                                                                                 <  2:2761497/150‑1 (MQ=255)
      tGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTa                                                                                                                                                 <  2:55583/150‑1 (MQ=255)
      tGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTa                                                                                                                                                 <  2:3017656/150‑1 (MQ=255)
      tGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTa                                                                                                                                                 <  1:1632107/150‑1 (MQ=255)
       gAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTCGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTaa                                                                                                                                                >  2:2314198/1‑150 (MQ=255)
        aaaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAAc                                                                                                                                               >  1:3228664/1‑150 (MQ=255)
         aaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACg                                                                                                                                              <  2:744715/150‑1 (MQ=255)
          atatGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTTTTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACgg                                                                                                                                             <  1:1062146/150‑1 (MQ=255)
          atatGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACgg                                                                                                                                             >  2:3095732/1‑150 (MQ=255)
          atatGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACgg                                                                                                                                             <  1:4494454/150‑1 (MQ=255)
             tGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtga                                                                                                                                          <  1:1823784/150‑1 (MQ=255)
             tGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtga                                                                                                                                          <  1:2996771/150‑1 (MQ=255)
              gCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtgat                                                                                                                                         <  1:1747652/150‑1 (MQ=255)
              gCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtgat                                                                                                                                         <  1:395262/150‑1 (MQ=255)
                   cGATGTGGTAGGGCGTATTCAGCGGCTTATTGAAGCGATCCTGTCGCATATTGTTGGTAACGGTAATATTCACTCCCAGGTTACGGCGCAGCTGGACTTCGCAATTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAAtc                                                                                                                                    <  2:2312709/150‑1 (MQ=255)
                      tGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCa                                                                                                                                 >  2:2418375/1‑150 (MQ=255)
                        tCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATg                                                                                                                               <  2:3509920/150‑1 (MQ=255)
                        tCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATg                                                                                                                               >  2:1273245/1‑150 (MQ=255)
                           aaGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcgg                                                                                                                            >  1:2226396/1‑150 (MQ=255)
                             ggCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcggcg                                                                                                                          >  1:1627647/1‑150 (MQ=255)
                             ggCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcggcg                                                                                                                          >  1:2877108/1‑150 (MQ=255)
                              gCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGgcgc                                                                                                                         >  2:764316/1‑150 (MQ=255)
                                cGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCtt                                                                                                                       <  2:3649304/150‑1 (MQ=255)
                                 gTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTc                                                                                                                      >  1:3393286/1‑150 (MQ=255)
                                     tCAGCGGCGTATTGAAGCGATCCTGTCGCCTTTTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTc                                                                                                                  <  1:4269296/150‑1 (MQ=255)
                                       aGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCAc                                                                                                                >  1:3368082/1‑150 (MQ=255)
                                               aTTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCtt                                                                                                        >  1:2444568/1‑150 (MQ=255)
                                                ttGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTa                                                                                                       <  2:3887925/150‑1 (MQ=255)
                                                  gAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAAt                                                                                                     <  1:1991702/150‑1 (MQ=255)
                                                    aGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATga                                                                                                   <  2:315965/150‑1 (MQ=255)
                                                    aGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATga                                                                                                   >  2:2301374/1‑150 (MQ=255)
                                                          ccTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcga                                                                                             >  1:1935367/1‑150 (MQ=255)
                                                            tGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcgagc                                                                                           >  1:1705812/1‑150 (MQ=255)
                                                            tGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcgagc                                                                                           >  2:3212174/1‑150 (MQ=255)
                                                             gTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCa                                                                                          <  2:1378820/150‑1 (MQ=255)
                                                                gCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAg                                                                                       >  2:811238/1‑150 (MQ=255)
                                                                gCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAg                                                                                       >  2:1938651/1‑150 (MQ=255)
                                                                  cTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCg                                                                                     <  2:1316734/150‑1 (MQ=255)
                                                                    aTTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGt                                                                                   >  2:1840559/1‑150 (MQ=255)
                                                                    aTTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGt                                                                                   >  2:1114486/1‑150 (MQ=255)
                                                                     ttgttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGtt                                                                                  >  1:2234955/1‑150 (MQ=255)
                                                                         tgGTAACGGTAATTTTCACGCCCAGGTTACGGCGCAGCTGTACTTCGCCATTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGATTCGTTCACGCCAGATTAATGAGAGCGAGCAAAGCGGTTCCgg                                                                              <  1:4332929/150‑1 (MQ=255)
                                                                          ggTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGt                                                                             >  1:2184544/1‑150 (MQ=255)
                                                                            tAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTa                                                                           <  1:514143/150‑1 (MQ=255)
                                                                             aaCGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTAt                                                                          >  2:4150894/1‑150 (MQ=255)
                                                                             aaCGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTAt                                                                          >  2:1622740/1‑150 (MQ=255)
                                                                                ggTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCg                                                                       >  2:4479181/1‑150 (MQ=255)
                                                                                ggTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCg                                                                       <  2:2031523/150‑1 (MQ=255)
                                                                                 gTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCgg                                                                      <  2:1610543/150‑1 (MQ=255)
                                                                                    atatTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGggcg                                                                   >  1:2911799/1‑150 (MQ=255)
                                                                                     tatTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGACAGCTTAATGAGAGAGAGCAAAGCGGTTACGGTTATCCGggcgg                                                                  >  1:948769/1‑150 (MQ=255)
                                                                                      atTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGggcggc                                                                 >  1:268780/1‑150 (MQ=255)
                                                                                      atTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGggcggc                                                                 >  2:4311467/1‑150 (MQ=255)
                                                                                       ttCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGgcggcg                                                                >  1:4382380/1‑150 (MQ=255)
                                                                                        tCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGt                                                               <  2:2660124/150‑1 (MQ=255)
                                                                                           cGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTAcc                                                            >  2:1110077/1‑150 (MQ=255)
                                                                                             cccAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCgg                                                          >  2:101300/1‑150 (MQ=255)
                                                                                             cccAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCgg                                                          <  2:3071096/150‑1 (MQ=255)
                                                                                                aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCggggg                                                       >  2:4035912/1‑150 (MQ=255)
                                                                                                 ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGc                                                      <  2:3942172/150‑1 (MQ=255)
                                                                                                    tACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGtt                                                   <  1:3362436/150‑1 (MQ=255)
                                                                                                    tACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGtt                                                   <  1:1739511/150‑1 (MQ=255)
                                                                                                       ggCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTc                                                <  2:3362395/150‑1 (MQ=255)
                                                                                                        gcgcAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCg                                               <  1:2445578/150‑1 (MQ=255)
                                                                                                         cgcAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGa                                              >  2:976409/1‑150 (MQ=255)
                                                                                                           cAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAAt                                            >  1:3979835/1‑150 (MQ=255)
                                                                                                           cAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAAt                                            <  1:4528483/150‑1 (MQ=255)
                                                                                                            aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATc                                           <  1:1308237/150‑1 (MQ=255)
                                                                                                            aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATc                                           >  2:688735/1‑150 (MQ=255)
                                                                                                            aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATc                                           <  1:3592149/150‑1 (MQ=255)
                                                                                                            aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATc                                           >  1:747867/1‑150 (MQ=255)
                                                                                                               tGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAAc                                        >  1:3438446/1‑150 (MQ=255)
                                                                                                                 gACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCg                                      <  2:145766/150‑1 (MQ=255)
                                                                                                                 gACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCg                                      >  2:2556283/1‑150 (MQ=255)
                                                                                                                     tCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCAc                                  <  1:2968022/150‑1 (MQ=255)
                                                                                                                         cAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGc                              >  1:715453/1‑150 (MQ=255)
                                                                                                                          aGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGGTGTCGAATCAACCGGCACCTGCg                             >  2:3576715/1‑150 (MQ=255)
                                                                                                                              aaGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATa                         <  2:399617/150‑1 (MQ=255)
                                                                                                                               aGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATaa                        <  2:1683870/150‑1 (MQ=255)
                                                                                                                               aGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATaa                        <  1:1418193/150‑1 (MQ=255)
                                                                                                                               aGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGTCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATaa                        >  1:3758363/1‑150 (MQ=255)
                                                                                                                                gAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAc                       <  1:2440433/150‑1 (MQ=255)
                                                                                                                                    aaaCGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACgcgc                   >  2:211838/1‑150 (MQ=255)
                                                                                                                                       cGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCaa                >  2:3458021/1‑150 (MQ=255)
                                                                                                                                         gaagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATc              >  2:347099/1‑150 (MQ=255)
                                                                                                                                          aagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCa             >  1:1157925/1‑150 (MQ=255)
                                                                                                                                          aagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCa             <  1:2808816/150‑1 (MQ=255)
                                                                                                                                          aagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCa             >  1:1260375/1‑150 (MQ=255)
                                                                                                                                           agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  2:3818846/1‑150 (MQ=255)
                                                                                                                                           agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  1:303744/1‑150 (MQ=255)
                                                                                                                                           agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  1:151457/1‑150 (MQ=255)
                                                                                                                                              aCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCAc         <  1:1114486/150‑1 (MQ=255)
                                                                                                                                               cAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACg        <  1:3851853/150‑1 (MQ=255)
                                                                                                                                                aGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGc       >  1:2393879/1‑150 (MQ=255)
                                                                                                                                                aGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGc       >  1:2843646/1‑150 (MQ=255)
                                                                                                                                                 gTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGcc      >  2:2171129/1‑150 (MQ=255)
                                                                                                                                                   aTCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTc    >  2:2877777/1‑150 (MQ=255)
                                                                                                                                                     cGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCg  <  2:2488735/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCG  >  CP009273/2007398‑2007696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A