Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,007,547 C→A 41.5% R280S (CGC→AGC)  fliF → flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,007,5470CA41.5% 253.9 / 283.8 238R280S (CGC→AGC) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base C (67/72);  new base A (53/46);  total (120/118)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 245 total aligned reads displayed.

CTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCG  >  CP009273/2007398‑2007696
                                                                                                                                                     |                                                                                                                                                     
cTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATc                                                                                                                                                       <  2:3919551/150‑1 (MQ=255)
cTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATc                                                                                                                                                       >  1:1374597/1‑150 (MQ=255)
 tCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCg                                                                                                                                                      >  2:1880220/1‑150 (MQ=255)
     ttGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCt                                                                                                                                                  <  1:1671876/150‑1 (MQ=255)
       gAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTaa                                                                                                                                                >  2:2797195/1‑150 (MQ=255)
        aaaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAAc                                                                                                                                               >  2:2109186/1‑150 (MQ=255)
        aaaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAAc                                                                                                                                               <  1:2150178/150‑1 (MQ=255)
         aaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACg                                                                                                                                              <  2:376624/150‑1 (MQ=255)
         aaTATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACg                                                                                                                                              >  2:3223355/1‑150 (MQ=255)
          atatGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACgg                                                                                                                                             >  2:8396/1‑150 (MQ=255)
            atGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtg                                                                                                                                           >  2:2690787/1‑150 (MQ=255)
                cAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGtgatga                                                                                                                                       >  1:200383/1‑150 (MQ=255)
                   cGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAAtc                                                                                                                                    >  1:2674129/1‑150 (MQ=255)
                    gATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAAtct                                                                                                                                   <  1:3626739/150‑1 (MQ=255)
                    gATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAAtct                                                                                                                                   <  1:3250631/150‑1 (MQ=255)
                    gATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAAtct                                                                                                                                   <  1:3971808/150‑1 (MQ=255)
                      tGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCa                                                                                                                                 >  1:2399843/1‑150 (MQ=255)
                       gTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCAt                                                                                                                                <  1:1665329/150‑1 (MQ=255)
                         cGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgc                                                                                                                              >  1:856642/1‑150 (MQ=255)
                          gAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcg                                                                                                                             >  2:1054809/1‑150 (MQ=255)
                          gAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcg                                                                                                                             <  1:3549565/150‑1 (MQ=255)
                          gAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATgcg                                                                                                                             >  2:3649441/1‑150 (MQ=255)
                          gAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATgcg                                                                                                                             <  1:1258023/150‑1 (MQ=255)
                          gAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATgcg                                                                                                                             >  2:1425171/1‑150 (MQ=255)
                           aaGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATgcgg                                                                                                                            <  2:4116796/150‑1 (MQ=255)
                                cGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCtt                                                                                                                       >  1:495275/1‑150 (MQ=255)
                                 gTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTc                                                                                                                      <  2:923570/150‑1 (MQ=255)
                                 gTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTc                                                                                                                      <  1:388145/150‑1 (MQ=255)
                                   aTTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGt                                                                                                                    >  2:3014452/1‑150 (MQ=255)
                                     tCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTc                                                                                                                  >  1:2589420/1‑150 (MQ=255)
                                     tCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTc                                                                                                                  >  2:3174298/1‑150 (MQ=255)
                                      cAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCa                                                                                                                 >  1:3434600/1‑150 (MQ=255)
                                       aGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCAc                                                                                                                <  2:850295/150‑1 (MQ=255)
                                       aGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCAc                                                                                                                <  1:4138536/150‑1 (MQ=255)
                                            cgTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAg                                                                                                           <  2:3264371/150‑1 (MQ=255)
                                             gTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGc                                                                                                          >  1:2274414/1‑150 (MQ=255)
                                                   aaGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATg                                                                                                    <  2:3343548/150‑1 (MQ=255)
                                                    aGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATga                                                                                                   >  2:284202/1‑150 (MQ=255)
                                                    aGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATga                                                                                                   <  2:1587161/150‑1 (MQ=255)
                                                     gCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATgag                                                                                                  <  2:2010449/150‑1 (MQ=255)
                                                     gCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATgag                                                                                                  <  1:2370587/150‑1 (MQ=255)
                                                     gCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATgag                                                                                                  <  2:2010400/150‑1 (MQ=255)
                                                       gATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGagag                                                                                                <  1:821818/150‑1 (MQ=255)
                                                         tCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcg                                                                                              <  2:70406/150‑1 (MQ=255)
                                                         tCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcg                                                                                              <  1:1135335/150‑1 (MQ=255)
                                                         tCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAgagcg                                                                                              >  2:3766082/1‑150 (MQ=255)
                                                                gCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAg                                                                                       <  1:546038/150‑1 (MQ=255)
                                                                  cTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCg                                                                                     >  1:1225417/1‑150 (MQ=255)
                                                                       gttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTcc                                                                                >  2:2156608/1‑150 (MQ=255)
                                                                       gttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTcc                                                                                >  2:782009/1‑150 (MQ=255)
                                                                        ttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCg                                                                               <  2:2599496/150‑1 (MQ=255)
                                                                        ttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCg                                                                               <  2:2175978/150‑1 (MQ=255)
                                                                          ggTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGt                                                                             <  1:3634501/150‑1 (MQ=255)
                                                                           gTAACGGTAATATTCCCGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGtt                                                                            <  2:3139410/150‑1 (MQ=255)
                                                                           gTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGtt                                                                            <  2:3555483/150‑1 (MQ=255)
                                                                            tAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTa                                                                           <  1:103874/150‑1 (MQ=255)
                                                                             aaCGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCACAGCGGTTCCGGTTAt                                                                          >  2:1092972/1‑150 (MQ=255)
                                                                             aaCGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTAt                                                                          >  1:2747319/1‑150 (MQ=255)
                                                                               cGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATcc                                                                        >  2:3069132/1‑150 (MQ=255)
                                                                                    atatTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGggcg                                                                   >  1:397818/1‑150 (MQ=255)
                                                                                      atTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGggcggc                                                                 >  2:2392580/1‑150 (MQ=255)
                                                                                       ttCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGgcggcg                                                                <  1:2110099/150‑1 (MQ=255)
                                                                                              ccAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCggg                                                         >  2:3378914/1‑150 (MQ=255)
                                                                                                aGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCggggg                                                       >  2:2268741/1‑150 (MQ=255)
                                                                                                 ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGc                                                      <  2:1708375/150‑1 (MQ=255)
                                                                                                 ggTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGc                                                      <  1:378656/150‑1 (MQ=255)
                                                                                                     aCGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGGTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTg                                                  <  2:3869677/150‑1 (MQ=255)
                                                                                                       gtcgcAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACATTATCGCCCTAACGGTGATGACTCTCATGCGGCGCTTCGTTCCCGCCAGCTTTATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTc                                                <  2:1653063/148‑1 (MQ=255)
                                                                                                            aGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATc                                           >  2:936881/1‑150 (MQ=255)
                                                                                                             gCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCa                                          <  1:2512509/150‑1 (MQ=255)
                                                                                                             gCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCGTCGGTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCa                                          <  2:2409742/150‑1 (MQ=255)
                                                                                                               tGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAAc                                        >  2:399415/1‑150 (MQ=255)
                                                                                                                ggACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAAcc                                       >  1:3286369/1‑150 (MQ=255)
                                                                                                                 gACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCg                                      <  2:1015188/150‑1 (MQ=255)
                                                                                                                  aCTTCGCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCgg                                     <  1:3732684/150‑1 (MQ=255)
                                                                                                                     tCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCAc                                  <  1:303172/150‑1 (MQ=255)
                                                                                                                       gCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCt                                >  1:925782/1‑150 (MQ=255)
                                                                                                                       gCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCt                                >  1:711110/1‑150 (MQ=255)
                                                                                                                       gCCAGTAAAGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCt                                <  1:3564535/150‑1 (MQ=255)
                                                                                                                           gTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGa                            <  1:1901253/150‑1 (MQ=255)
                                                                                                                              aaGAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATa                         <  2:3534364/150‑1 (MQ=255)
                                                                                                                                gAACAAACGGAAGAACAGTATCGCCATAACGTTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGATCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAc                       <  1:69208/150‑1 (MQ=255)
                                                                                                                                gAACAAACGGAAGAACAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAc                       >  1:2719727/1‑150 (MQ=255)
                                                                                                                                  aCAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAcgc                     >  1:1388735/1‑150 (MQ=255)
                                                                                                                                  aCAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAcgc                     >  1:538066/1‑150 (MQ=255)
                                                                                                                                   cAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAAcgcg                    <  1:1271025/150‑1 (MQ=255)
                                                                                                                                      aCGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCa                 <  2:765027/150‑1 (MQ=255)
                                                                                                                                         gaagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATc              >  2:2321855/1‑150 (MQ=255)
                                                                                                                                         gaagaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATc              <  2:4038667/150‑1 (MQ=255)
                                                                                                                                           agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  2:3147725/1‑150 (MQ=255)
                                                                                                                                           agaaCAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  2:1473000/1‑150 (MQ=255)
                                                                                                                                           agaaCAGTATAGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAg            >  2:696033/1‑150 (MQ=255)
                                                                                                                                               cAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCCATCAGCACg        >  1:4048589/1‑150 (MQ=255)
                                                                                                                                               cAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACg        >  1:2028524/1‑150 (MQ=255)
                                                                                                                                               cAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACg        >  2:3623099/1‑150 (MQ=255)
                                                                                                                                                aGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGc       >  2:2838345/1‑150 (MQ=255)
                                                                                                                                                  tataGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCt     >  2:1534880/1‑150 (MQ=255)
                                                                                                                                                  tATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAATCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCt     >  1:2976808/1‑150 (MQ=255)
                                                                                                                                                     cGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCg  >  1:2847930/1‑150 (MQ=255)
                                                                                                                                                     cGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCg  >  1:423744/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCGAATAACGCGCCAATCAGCACGCCTCCG  >  CP009273/2007398‑2007696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A