Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 89.8% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA89.8% 734.7 / 67.9 271R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (13/15);  new base A (113/130);  total (126/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 276 total aligned reads displayed.

CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCC  >  CP009273/3110604‑3110900
                                                                                                                                                   |                                                                                                                                                     
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                     <  1:484416/150‑1 (MQ=255)
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                     <  1:922904/150‑1 (MQ=255)
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                     <  1:4436645/150‑1 (MQ=255)
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                     <  2:1167020/150‑1 (MQ=255)
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAAAGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                     >  1:3534505/1‑150 (MQ=255)
  ggCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaa                                                                                                                                                   <  2:2072879/150‑1 (MQ=255)
   gCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGgcaaagcaaa                                                                                                                                                  <  2:643261/150‑1 (MQ=255)
        cgcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCt                                                                                                                                             >  2:2645156/1‑150 (MQ=255)
        cgcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCt                                                                                                                                             >  1:1811680/1‑150 (MQ=255)
         gcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtg                                                                                                                                            <  2:3657589/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCtgt                                                                                                                                           <  2:3366694/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCtgt                                                                                                                                           <  1:307291/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                           <  2:4386530/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                           <  2:3853581/150‑1 (MQ=255)
           gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                          <  1:2875896/150‑1 (MQ=255)
           gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                          <  1:3069664/150‑1 (MQ=255)
                gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                     <  2:1852734/150‑1 (MQ=255)
                gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                     <  1:3119994/150‑1 (MQ=255)
                gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                     <  1:1070001/150‑1 (MQ=255)
                 cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAgtg                                                                                                                                    <  2:3148194/150‑1 (MQ=255)
                 cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtg                                                                                                                                    <  1:2533246/150‑1 (MQ=255)
                    ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                                 <  1:3171648/150‑1 (MQ=255)
                    ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                                 <  2:2942077/150‑1 (MQ=255)
                        ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTa                                                                                                                             >  2:1969587/1‑150 (MQ=255)
                         tATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAc                                                                                                                            >  2:4531989/1‑150 (MQ=255)
                          aTCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                           <  2:941390/150‑1 (MQ=255)
                            cgtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGa                                                                                                                         >  2:3037850/1‑150 (MQ=255)
                                  aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCa                                                                                                                   <  1:1184294/150‑1 (MQ=255)
                                   cAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAt                                                                                                                  <  1:2095858/150‑1 (MQ=255)
                                        aGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                             >  1:331979/1‑150 (MQ=255)
                                        aGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                             >  2:2280471/1‑150 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                           >  2:4013761/1‑150 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                           >  1:1360281/1‑150 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                           <  1:2648672/150‑1 (MQ=255)
                                             agcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGccc                                                                                                        <  1:22070/150‑1 (MQ=255)
                                              gcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCt                                                                                                       <  2:860641/150‑1 (MQ=255)
                                               caCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTc                                                                                                      <  1:3942649/150‑1 (MQ=255)
                                               caCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATATTTATTAAAGCCCCCGTTCCCGCAGTAACCGTTCGGGCACAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTTACCTc                                                                                                      >  1:1927620/1‑150 (MQ=255)
                                                  gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAcc                                                                                                   <  1:1437243/150‑1 (MQ=255)
                                                   ttCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCGGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCg                                                                                                  >  2:2763820/1‑150 (MQ=255)
                                                      tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                               >  2:2075101/1‑150 (MQ=255)
                                                      tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                               >  2:3770627/1‑150 (MQ=255)
                                                      tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAAAACCATCAGTTTGCCCTCACCGACt                                                                                               >  2:1486217/1‑150 (MQ=255)
                                                          tGAAGTTCTTCACGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGcc                                                                                           <  1:4020007/150‑1 (MQ=255)
                                                              gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAg                                                                                       <  1:1806696/150‑1 (MQ=255)
                                                              gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTAAACAGCCCATCTTTATTAAAGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCGCACCCACTTGACCCAg                                                                                       >  1:3795497/1‑150 (MQ=255)
                                                                 cttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGa                                                                                    <  1:4467793/150‑1 (MQ=255)
                                                                 cttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGa                                                                                    >  2:2874489/1‑150 (MQ=255)
                                                                   tcATGTCATCCGGGTCGCTGTTTAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAACGATAACCATCAGTTTGCCCTCACCGACATTCCCCAGCGaaa                                                                                  >  1:2750965/1‑150 (MQ=255)
                                                                    cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGaaac                                                                                 <  2:2346398/150‑2 (MQ=255)
                                                                        tCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAAc                                                                             <  2:161918/150‑1 (MQ=255)
                                                                           tCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGc                                                                          <  2:1460519/150‑1 (MQ=255)
                                                                               ggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGt                                                                      >  2:3569196/1‑150 (MQ=255)
                                                                               ggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGt                                                                      >  2:3380349/1‑150 (MQ=255)
                                                                               ggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGt                                                                      >  2:1283662/1‑150 (MQ=255)
                                                                                    cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAg                                                                 >  2:1563662/1‑150 (MQ=255)
                                                                                      gTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGt                                                               >  1:970987/1‑150 (MQ=255)
                                                                                       ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTg                                                              >  2:1616348/1‑150 (MQ=255)
                                                                                       ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTg                                                              >  1:4194604/1‑150 (MQ=255)
                                                                                        tGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTgg                                                             <  2:2892696/150‑1 (MQ=255)
                                                                                             gTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTa                                                        >  2:2644311/1‑150 (MQ=255)
                                                                                               acacTGCCCATCTTTATTCCCGCCCCCGTTCCCGCTGTAACCGTTGGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATACCCATCTGTTTGCCCTCCCCGACTTGCCCCCGCTAAAAAAACGGCAGGTTAAAGGTGGCGGTATc                                                      <  1:572971/150‑1 (MQ=255)
                                                                                                 acTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgc                                                    >  2:747100/1‑150 (MQ=255)
                                                                                                  cTGCCAAACTTTATAACCCCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTTTCGTGTGGGTTACCGATAACCATCATTTTGCCCACCCCGACTGGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                                   <  1:103993/150‑1 (MQ=255)
                                                                                                   tGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcgc                                                  >  1:4452071/1‑150 (MQ=255)
                                                                                                        aTCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAc                                             >  2:2036024/1‑150 (MQ=255)
                                                                                                            ttattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAACATGCTGTTGTAGTGTGGGTTACCGATAACCATCCGTTTGCCCTCACCGACTTGCCCCCGCGAAATAAACGGCAGGTGAAAGGTGGCGGTATCGCGCGGAACGttt                                         >  1:1986022/1‑150 (MQ=255)
                                                                                                            ttattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                         >  1:4262249/1‑150 (MQ=255)
                                                                                                             tattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                        <  2:2491665/150‑1 (MQ=255)
                                                                                                              attaACGCCCCCGTTCCCGCTGTAACCGTTCGGGCAAAGCAAAATGCTTTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTc                                       <  2:2364032/150‑1 (MQ=255)
                                                                                                              attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTc                                       <  2:103199/150‑1 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTACCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                     <  2:1747556/150‑1 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                     >  1:836428/1‑150 (MQ=255)
                                                                                                                 aaCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCgg                                    >  1:3450891/1‑150 (MQ=255)
                                                                                                                 aaCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCgg                                    >  1:3718256/1‑150 (MQ=255)
                                                                                                                    gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                                 >  2:1833589/1‑150 (MQ=255)
                                                                                                                        ccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaaca                             <  2:1360281/150‑1 (MQ=255)
                                                                                                                          gTTCCAGCTGTAACCTTTCGGGCAAAGAAAATTGCTGTTGTTGTGTGGGTTACCGATAACCATAATTTTGCCCTCACCGAATTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                           <  2:3446694/150‑1 (MQ=255)
                                                                                                                          gTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                           <  1:4071694/150‑1 (MQ=255)
                                                                                                                          gTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                           <  2:1111317/150‑1 (MQ=255)
                                                                                                                           ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                          >  2:468851/1‑150 (MQ=255)
                                                                                                                            tCCTGCTGTAACCGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCTGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                         <  1:3956623/150‑1 (MQ=255)
                                                                                                                             ccAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                        >  1:2328425/1‑150 (MQ=255)
                                                                                                                              cAGCTGTACCGGTTCGGGCAAAGAAAAATGCTGTTTTTGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                       <  1:1660760/150‑1 (MQ=255)
                                                                                                                              cAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                       <  2:966462/150‑1 (MQ=255)
                                                                                                                              cAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                       <  2:2396692/150‑1 (MQ=255)
                                                                                                                              cAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                       >  1:413799/1‑150 (MQ=255)
                                                                                                                               cgCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                      <  2:3978517/149‑1 (MQ=255)
                                                                                                                                    tAACCGTTCGGGCAAAGCAAATTGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCt                 <  2:4319430/150‑1 (MQ=255)
                                                                                                                                    tAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCt                 >  2:2580674/1‑150 (MQ=255)
                                                                                                                                    tAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCt                 >  2:695147/1‑150 (MQ=255)
                                                                                                                                      aCCGTTCGGGAAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTc               <  2:163992/150‑1 (MQ=255)
                                                                                                                                       ccGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTcc              <  2:4137813/150‑1 (MQ=255)
                                                                                                                                          ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTa           >  1:1284237/1‑150 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAACTGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt         <  1:4151842/150‑1 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt         >  2:3922032/1‑150 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt         <  2:161041/150‑1 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt         >  1:1061137/1‑150 (MQ=255)
                                                                                                                                                cAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAc     >  2:2174918/1‑150 (MQ=255)
                                                                                                                                                   cGCAAAGTGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTGGCCCCAGCGAAATCCACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGcc  <  1:3680355/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCC  >  CP009273/3110604‑3110900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCC  >  CP009273/3110603‑3110900
                                                                                                                                                    |                                                                                                                                                     
GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAG                                                                                                                                                      <  SRR19973080.3666813/150‑1 (MQ=60)
GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAG                                                                                                                                                      <  SRR19973080.3665081/150‑1 (MQ=60)
GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAG                                                                                                                                                      <  SRR19973080.2492865/150‑1 (MQ=60)
 CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                     <  SRR19973080.928558/150‑1 (MQ=60)
 CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                     <  SRR19973080.487497/150‑1 (MQ=60)
 CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                     <  SRR19973080.4460784/150‑1 (MQ=60)
 CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                     <  SRR19973080.4460737/150‑1 (MQ=60)
 CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAAAGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                     >  SRR19973080.3554057/1‑150 (MQ=60)
  TGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCA                                                                                                                                                    <  SRR19973080.3236094/150‑1 (MQ=60)
    GCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAA                                                                                                                                                  <  SRR19973080.337666/150‑1 (MQ=60)
         CGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCT                                                                                                                                             <  SRR19973080.1951151/150‑1 (MQ=60)
         CGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCT                                                                                                                                             >  SRR19973080.1821958/1‑150 (MQ=60)
           CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGT                                                                                                                                           <  SRR19973080.309253/150‑1 (MQ=60)
           CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGT                                                                                                                                           <  SRR19973080.309217/150‑1 (MQ=60)
           CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGT                                                                                                                                           <  SRR19973080.309237/150‑1 (MQ=60)
           CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGT                                                                                                                                           >  SRR19973080.3225672/1‑150 (MQ=60)
            GTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTT                                                                                                                                          <  SRR19973080.3087399/150‑1 (MQ=60)
            GTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTT                                                                                                                                          <  SRR19973080.3086383/150‑1 (MQ=60)
            GTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTT                                                                                                                                          <  SRR19973080.2891638/150‑1 (MQ=60)
                GGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAG                                                                                                                                      <  SRR19973080.2280399/150‑1 (MQ=60)
                 GCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGT                                                                                                                                     <  SRR19973080.3137147/150‑1 (MQ=60)
                 GCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGT                                                                                                                                     <  SRR19973080.1076235/150‑1 (MQ=60)
                  CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                    <  SRR19973080.271399/150‑1 (MQ=60)
                  CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                    <  SRR19973080.2547160/150‑1 (MQ=60)
                  CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                    <  SRR19973080.1922729/150‑1 (MQ=60)
                  CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                    <  SRR19973080.1920870/150‑1 (MQ=60)
                   TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGT                                                                                                                                   <  SRR19973080.1067314/150‑1 (MQ=60)
                   TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGT                                                                                                                                   >  SRR19973080.3547734/1‑150 (MQ=60)
                   TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGT                                                                                                                                   >  SRR19973080.2897877/1‑150 (MQ=60)
                     CCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGG                                                                                                                                 <  SRR19973080.3189139/150‑1 (MQ=60)
                     CCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGG                                                                                                                                 <  SRR19973080.3189115/150‑1 (MQ=60)
                        TTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTT                                                                                                                              <  SRR19973080.2780436/150‑1 (MQ=60)
                                  GACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACC                                                                                                                    <  SRR19973080.316760/150‑1 (MQ=60)
                                   ACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCA                                                                                                                   <  SRR19973080.1191384/150‑1 (MQ=60)
                                   ACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCA                                                                                                                   <  SRR19973080.1192365/150‑1 (MQ=60)
                                    CAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAT                                                                                                                  >  SRR19973080.4318707/1‑150 (MQ=60)
                                    CAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAT                                                                                                                  <  SRR19973080.2107691/150‑1 (MQ=60)
                                    CAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCGGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCAT                                                                                                                  >  SRR19973080.2032157/1‑150 (MQ=60)
                                         AGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTT                                                                                                             >  SRR19973080.333990/1‑150 (MQ=60)
                                           CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTG                                                                                                           >  SRR19973080.1368421/1‑150 (MQ=60)
                                           CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTG                                                                                                           <  SRR19973080.387909/150‑1 (MQ=60)
                                           CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTG                                                                                                           <  SRR19973080.2662958/150‑1 (MQ=60)
                                              AGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCC                                                                                                        <  SRR19973080.22321/150‑1 (MQ=60)
                                               GCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCT                                                                                                       <  SRR19973080.42866/150‑1 (MQ=60)
                                                CACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTC                                                                                                      <  SRR19973080.3964385/150‑1 (MQ=60)
                                                CACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATATTTATTAAAGCCCCCGTTCCCGCAGTAACCGTTCGGGCACAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTTACCTC                                                                                                      >  SRR19973080.1938505/1‑150 (MQ=60)
                                                   GTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACC                                                                                                   <  SRR19973080.1445633/150‑1 (MQ=60)
                                                    TTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCG                                                                                                  <  SRR19973080.2360668/150‑1 (MQ=60)
                                                      CTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTTAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTCGGGCAAATCAAAATTCTGTTGTATTGTGGGTTAACGATAACCATCAGTTTtcccacaaccac                                                                                                >  SRR19973080.85444/1‑138 (MQ=60)
                                                      CTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCACACCGAC                                                                                                >  SRR19973080.4017726/1‑150 (MQ=60)
                                                      CTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTAACGATAACCATCAGTTTTCCCTCACCGAC                                                                                                >  SRR19973080.4021113/1‑150 (MQ=60)
                                                      CTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCACTTTGCCCTCACCGAC                                                                                                >  SRR19973080.2324451/1‑150 (MQ=60)
                                                        CCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTT                                                                                              <  SRR19973080.4483638/150‑1 (MQ=60)
                                                        CCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTT                                                                                              >  SRR19973080.2900870/1‑150 (MQ=60)
                                                           TGAAGTTCTTCACGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCC                                                                                           <  SRR19973080.4042048/150‑1 (MQ=60)
                                                            GAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAAAGCCCCCCTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAAGCTGTTTTAGTGTGGGTTACCGATAAACAACAGTTTGCCCTCACCCACTTGCCC                                                                                          >  SRR19973080.1541226/1‑150 (MQ=60)
                                                             AAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTGTATTCCCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCC                                                                                         <  SRR19973080.2565455/150‑1 (MQ=60)
                                                               GTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAG                                                                                       <  SRR19973080.1816963/150‑1 (MQ=60)
                                                               GTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAG                                                                                       >  SRR19973080.1733058/1‑150 (MQ=60)
                                                               GTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTAAACAGCCCATCTTTATTAAAGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCGCACCCACTTGACCCAG                                                                                       >  SRR19973080.3816188/1‑150 (MQ=60)
                                                                  CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                    <  SRR19973080.4092351/150‑1 (MQ=60)
                                                                  CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                    <  SRR19973080.4491992/150‑1 (MQ=60)
                                                                  CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                    <  SRR19973080.4491990/150‑1 (MQ=60)
                                                                    TCATGTCATCCGGGTCGCTGTTTAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAACGATAACCATCAGTTTGCCCTCACCGACATTCCCCAGCGAAA                                                                                  >  SRR19973080.2766119/1‑150 (MQ=60)
                                                                    TCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAA                                                                                  >  SRR19973080.3721727/1‑150 (MQ=60)
                                                                     CATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAT                                                                                 >  SRR19973080.1821822/1‑150 (MQ=60)
                                                                          CATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACG                                                                            >  SRR19973080.1435804/1‑150 (MQ=60)
                                                                              CGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAG                                                                        <  SRR19973080.3478612/150‑1 (MQ=60)
                                                                              CGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAG                                                                        >  SRR19973080.4046291/1‑150 (MQ=60)
                                                                                 GTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTT                                                                     >  SRR19973080.3728674/1‑150 (MQ=60)
                                                                                   CGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGACATAAACGGCAGGTTAA                                                                   >  SRR19973080.2093392/1‑150 (MQ=60)
                                                                                    GCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAA                                                                  >  SRR19973080.419931/1‑150 (MQ=60)
                                                                                    GCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAA                                                                  >  SRR19973080.419511/1‑150 (MQ=60)
                                                                                    GCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAA                                                                  >  SRR19973080.611146/1‑150 (MQ=60)
                                                                                    GCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAA                                                                  >  SRR19973080.2465918/1‑150 (MQ=60)
                                                                                       GTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGT                                                               >  SRR19973080.976863/1‑150 (MQ=60)
                                                                                        TTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTG                                                              >  SRR19973080.4217385/1‑150 (MQ=60)
                                                                                         TGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGG                                                             >  SRR19973080.1652876/1‑150 (MQ=60)
                                                                                           AGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCG                                                           >  SRR19973080.2203169/1‑150 (MQ=60)
                                                                                             CGTACACTGCCAATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTGCGGGCAAAGAAAATTGCTGTTGTTGTGTGGGTTACCGATAACAATCTGTTTGCCCTCACCGAGTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGT                                                         <  SRR19973080.1309968/150‑1 (MQ=60)
                                                                                               TACACTCCCCATTTTTATTACCCCCCCCGTTCCAGCTGTAACCTTGCGGGAAAACAAAATTGCTGTTTTTGTGTGGTTTACCTATAACAATCTTTTGGCCCTCACCGACTGGCCCCAGCGAAAAACACGGCAGGTTAAAGGTGGCGGTAT                                                       <  SRR19973080.3845011/150‑1 (MQ=24)
                                                                                                ACACTGCCCATCTTTATTCCCGCCCCCGTTCCCGCTGTAACCGTTGGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATACCCATCTGTTTGCCCTCCCCGACTTGCCCCCGCTAAAAAAACGGCAGGTTAAAGGTGGCGGTATC                                                      <  SRR19973080.576552/150‑1 (MQ=60)
                                                                                                ACACTGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAACAAAAATGCTGTTGTCGTGTGGGTTACCTATAACCATCAGTTTGCCCACACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATC                                                      <  SRR19973080.576258/150‑1 (MQ=60)
                                                                                                   ctgccaaactttataacccCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTTTCGTGTGGGTTACCGATAACCATCATTTTGCCCACCCCGACTGGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCG                                                   <  SRR19973080.104933/131‑1 (MQ=60)
                                                                                                    TGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGC                                                  >  SRR19973080.4476218/1‑150 (MQ=60)
                                                                                                     ccccatctttttaccccCCCCCGTTCCCGGTGTAACGGTGCGGGCAACGCAAACTGCTGTTGTTGTGTGGTTTACCTATAACCATCAGTTTCCCCGCCCCGAGTTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                                 <  SRR19973080.1531120/133‑1 (MQ=60)
                                                                                                     GCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                                 >  SRR19973080.537793/1‑150 (MQ=60)
                                                                                                     GCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                                 >  SRR19973080.2810759/1‑150 (MQ=60)
                                                                                                       CCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTA                                               >  SRR19973080.3419029/1‑150 (MQ=60)
                                                                                                           CTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCTATAACCATCAGTTTGCCCCCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGT                                           <  SRR19973080.3787303/150‑1 (MQ=60)
                                                                                                           CTTTATTAACGCCCCCGTTCCAGCTGTACCCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGT                                           <  SRR19973080.3787322/150‑1 (MQ=60)
                                                                                                            TTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTT                                          >  SRR19973080.1210186/1‑150 (MQ=60)
                                                                                                             TTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAACATGCTGTTGTAGTGTGGGTTACCGATAACCATCCGTTTGCCCTCACCGACTTGCCCCCGCGAAATAAACGGCAGGTGAAAGGTGGCGGTATCGCGCGGAACGTTT                                         >  SRR19973080.1997304/1‑150 (MQ=60)
                                                                                                             TTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTT                                         >  SRR19973080.4285505/1‑150 (MQ=60)
                                                                                                             TTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTT                                         >  SRR19973080.4285507/1‑150 (MQ=60)
                                                                                                                TTACCGCCCCCGTTCCAGTTGTAACGGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTTTTGGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCC                                      <  SRR19973080.968792/150‑1 (MQ=60)
                                                                                                                 TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                     >  SRR19973080.841452/1‑150 (MQ=60)
                                                                                                                 TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                     >  SRR19973080.1976882/1‑150 (MQ=60)
                                                                                                                 TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                     >  SRR19973080.1409825/1‑150 (MQ=60)
                                                                                                                 TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGACCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAAGTTTTCCG                                     >  SRR19973080.841468/1‑150 (MQ=60)
                                                                                                                  AACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGG                                    >  SRR19973080.3738631/1‑150 (MQ=60)
                                                                                                                  AACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGG                                    >  SRR19973080.3469769/1‑150 (MQ=60)
                                                                                                                    CCCCCCCGTTCCAGGTGTACCCGTTCGGGCAAAGCAAATTGGTGTTGTTGGGGGGGTTACCGATAACCATCATTTTGCCCACACCGAATTGCCCCCGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCT                                  <  SRR19973080.48419/150‑1 (MQ=60)
                                                                                                                      CCCCCGTTCCAGCTTAAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACAATCAGTTTGCCCACACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCA                                <  SRR19973080.969355/150‑1 (MQ=60)
                                                                                                                      CCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCA                                <  SRR19973080.4167842/150‑1 (MQ=60)
                                                                                                                           GTTCCTGCGGTAACGGTTCGGGAAACGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTGGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAG                           <  SRR19973080.981354/150‑1 (MQ=60)
                                                                                                                           GTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAG                           <  SRR19973080.4093914/150‑1 (MQ=60)
                                                                                                                             TCCTGCTGTAACCGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCTGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAG                         <  SRR19973080.3978414/150‑1 (MQ=60)
                                                                                                                             TCCAGCTGTAACCGTTCGGGAAAAGAAAAATGCTGTTTTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAG                         <  SRR19973080.3978407/150‑1 (MQ=60)
                                                                                                                              CCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCTTCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGA                        <  SRR19973080.1095400/150‑1 (MQ=60)
                                                                                                                              CCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGA                        >  SRR19973080.2341367/1‑150 (MQ=60)
                                                                                                                               CAGCTGTACCGGTTCGGGCAAAGAAAAATGCTGTTTTTGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAA                       <  SRR19973080.1670466/150‑1 (MQ=60)
                                                                                                                               CAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAA                       >  SRR19973080.416306/1‑150 (MQ=60)
                                                                                                                                AGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCTGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAG                      <  SRR19973080.1083598/150‑1 (MQ=60)
                                                                                                                                           TTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTA           >  SRR19973080.4112484/1‑150 (MQ=60)
                                                                                                                                           TTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTA           >  SRR19973080.4112018/1‑150 (MQ=60)
                                                                                                                                           TTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTA           >  SRR19973080.1291998/1‑150 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAACTGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         <  SRR19973080.4174270/150‑1 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTCCCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         >  SRR19973080.2620883/1‑150 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         >  SRR19973080.1067933/1‑150 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         >  SRR19973080.3640246/1‑150 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         >  SRR19973080.1067344/1‑150 (MQ=60)
                                                                                                                                             CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT         >  SRR19973080.1067538/1‑150 (MQ=60)
                                                                                                                                              GGGCAAAGAAAAATGGTTTTGTTGTGTGGGTTACCTATAACCATAAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATG        <  SRR19973080.3949820/150‑1 (MQ=60)
                                                                                                                                              GGGAAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATG        <  SRR19973080.4556388/150‑1 (MQ=60)
                                                                                                                                               GGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT       >  SRR19973080.1730534/1‑150 (MQ=60)
                                                                                                                                                   ATGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATAAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGC   <  SRR19973080.3728100/150‑1 (MQ=60)
                                                                                                                                                    CGCAAAGTGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTGGCCCCAGCGAAATCCACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCC  <  SRR19973080.3700597/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCC  >  CP009273/3110603‑3110900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑