Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 100% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA99.5% 772.8 / ‑4.3 241R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (103/135);  total (104/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

TGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTA  >  CP009273/3110605‑3110896
                                                                                                                                                  |                                                                                                                                                 
tGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGGAACCGTTCGGGCAAAGc                                                                                                                                                 <  2:3197867/149‑1 (MQ=255)
    tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaaaa                                                                                                                                             <  1:2753202/149‑1 (MQ=255)
    tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaaaa                                                                                                                                             <  1:4178448/149‑1 (MQ=255)
    tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCaaaa                                                                                                                                             >  1:3388469/1‑149 (MQ=255)
         cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtg                                                                                                                                        <  1:3897482/149‑1 (MQ=255)
          gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                       >  2:501874/1‑149 (MQ=255)
          gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTAATCCGGGTCGCTGTTTAGCGTAAACAGACCAAAAATATTAAAGCCCCCCTTCCAGCTGTAACCCTTCGGGCAAAGCAAAAATCtgt                                                                                                                                       >  1:2859512/1‑149 (MQ=255)
           tCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                      >  2:1805760/1‑149 (MQ=255)
           tCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                      <  2:1376126/149‑1 (MQ=255)
              ggCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTa                                                                                                                                   >  2:2945486/1‑149 (MQ=255)
              ggCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTa                                                                                                                                   >  2:288753/1‑149 (MQ=255)
               gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAg                                                                                                                                  <  1:212804/149‑1 (MQ=255)
               gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAg                                                                                                                                  <  2:3036713/149‑1 (MQ=255)
                cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                 <  1:3499751/149‑1 (MQ=255)
                cTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgt                                                                                                                                 >  2:753318/1‑149 (MQ=255)
                 ttCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtg                                                                                                                                <  1:4671926/149‑1 (MQ=255)
                  tCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtgt                                                                                                                               >  1:2529144/1‑149 (MQ=255)
                   ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGtgtg                                                                                                                              >  2:4713684/1‑149 (MQ=255)
                    cATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                             >  1:12042/1‑149 (MQ=255)
                      tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                           <  1:2397357/149‑1 (MQ=255)
                      tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGGTCCAGCTGTAACCGGTCGGGCACAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                           >  2:356865/1‑149 (MQ=255)
                        tATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTa                                                                                                                         >  1:4505398/1‑149 (MQ=255)
                         aTCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAc                                                                                                                        <  2:2012427/149‑1 (MQ=255)
                          tcgtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                       <  2:4743364/149‑1 (MQ=255)
                              cgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                   <  1:4198242/149‑1 (MQ=255)
                                gNCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAc                                                                                                                 >  1:4018324/1‑149 (MQ=255)
                                gACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAc                                                                                                                 >  2:2094540/1‑149 (MQ=255)
                                 aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAcc                                                                                                                >  1:444117/1‑149 (MQ=255)
                                 aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAcc                                                                                                                <  1:262838/149‑1 (MQ=255)
                                    gATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATc                                                                                                             >  2:2535277/1‑149 (MQ=255)
                                     aTAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCa                                                                                                            <  1:1335572/149‑1 (MQ=255)
                                      tAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAg                                                                                                           >  2:220693/1‑149 (MQ=255)
                                        gcgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                         <  1:73747/149‑1 (MQ=255)
                                          gcagcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                       >  2:4468440/1‑149 (MQ=255)
                                             gcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAACATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGccc                                                                                                    >  2:3291600/1‑149 (MQ=255)
                                             gcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGccc                                                                                                    <  2:2869267/149‑1 (MQ=255)
                                               aCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTc                                                                                                  <  1:2249670/149‑1 (MQ=255)
                                               aCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTc                                                                                                  <  1:2249697/149‑1 (MQ=255)
                                                 gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAc                                                                                                <  1:1049304/149‑1 (MQ=255)
                                                 gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAc                                                                                                <  2:3157916/149‑1 (MQ=255)
                                                  ttCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAcc                                                                                               >  1:2199245/1‑149 (MQ=255)
                                                  ttCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGGTCCAGCTGTAACCGTTCGGGCAAAGCAAAATTCTGTTGTAGTGTGGGGTACCGATAACCATCAGTTTGCCCTCAcc                                                                                               >  1:4071849/1‑149 (MQ=255)
                                                     tcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGAc                                                                                            <  2:2401262/149‑1 (MQ=255)
                                                      ccATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACt                                                                                           <  2:3057551/149‑1 (MQ=255)
                                                             gTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGCCCCa                                                                                    >  1:500714/1‑149 (MQ=255)
                                                                cttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCg                                                                                 <  1:1089900/149‑1 (MQ=255)
                                                                   cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGaaa                                                                              <  2:1651035/149‑1 (MQ=255)
                                                                      gTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATaa                                                                           <  1:3642873/149‑1 (MQ=255)
                                                                      gTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATaa                                                                           <  1:4279851/149‑1 (MQ=255)
                                                                         aTCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACg                                                                        <  1:1574375/149‑1 (MQ=255)
                                                                          tCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACgg                                                                       <  1:1723171/149‑1 (MQ=255)
                                                                            cGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCa                                                                     <  2:4018110/149‑1 (MQ=255)
                                                                                tCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGtt                                                                 >  1:2288688/1‑149 (MQ=255)
                                                                                   cTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaaa                                                              >  2:1300289/1‑149 (MQ=255)
                                                                                    tGTTGAGCGTACACTGCCCATCTTTATTACCGCCCCCTTTCCTGCGGTAACCGTTCGGGCAAAGCAAAAGGCTGTTGTAGGGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAg                                                             <  2:2951762/149‑1 (MQ=255)
                                                                                     gTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                            <  2:2965944/149‑1 (MQ=255)
                                                                                      ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGt                                                           <  2:3334561/149‑1 (MQ=255)
                                                                                      ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGt                                                           <  2:1927289/149‑1 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGc                                                        <  2:3066488/149‑1 (MQ=255)
                                                                                         aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGc                                                        <  1:194479/149‑1 (MQ=255)
                                                                                            gTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGt                                                     <  2:1724866/149‑1 (MQ=255)
                                                                                             tACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTa                                                    >  1:1576202/1‑149 (MQ=255)
                                                                                              acacGGCCCATCTTTATTACCCCCCCCGTTCCCGCTGTAACCGTTCGGCCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTTTTTGCCCTCACCGACTGGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTAt                                                   <  1:1303122/149‑1 (MQ=255)
                                                                                               cacGGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCAGCATTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATc                                                  <  1:248940/149‑1 (MQ=255)
                                                                                                acTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcg                                                 >  1:4328154/1‑149 (MQ=255)
                                                                                                     ccATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGt                                            >  2:1727000/1‑149 (MQ=255)
                                                                                                     ccATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGt                                            >  2:4409475/1‑149 (MQ=255)
                                                                                                        tCTTTATTCACGCCCCCGTTCCAGCTGTACCCGTGGGGGCAAAGAAAATTGCTTTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCCCCGATTTGCCCCCGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAc                                         <  1:815565/149‑1 (MQ=255)
                                                                                                        tCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAc                                         >  1:2282103/1‑149 (MQ=255)
                                                                                                          tttATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGt                                       >  1:4450250/1‑149 (MQ=255)
                                                                                                          tttATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGt                                       >  2:2999518/1‑149 (MQ=255)
                                                                                                            tattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                     <  2:1576202/149‑1 (MQ=255)
                                                                                                             attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                    <  2:3450231/149‑1 (MQ=255)
                                                                                                             attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                    <  2:1061806/149‑1 (MQ=255)
                                                                                                              ttaCCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTc                                   <  2:4447026/149‑1 (MQ=255)
                                                                                                                  cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGc                               >  2:181266/1‑149 (MQ=255)
                                                                                                                  cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTGGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGc                               <  2:2417880/149‑1 (MQ=255)
                                                                                                                   gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTGTCCGGCt                              >  2:1161189/1‑149 (MQ=255)
                                                                                                                    cccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                             >  1:3280653/1‑149 (MQ=255)
                                                                                                                       ccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaac                          >  2:265378/1‑149 (MQ=255)
                                                                                                                         gTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaacaa                        >  2:2254233/1‑149 (MQ=255)
                                                                                                                          ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                       >  2:3853976/1‑149 (MQ=255)
                                                                                                                           tCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                      <  2:3702116/149‑1 (MQ=255)
                                                                                                                            ccAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGGTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                     <  2:559055/149‑1 (MQ=255)
                                                                                                                            ccAGCTGTAACCGTTCGGGCAAAGCAAAAGGCGGTTGTAGGGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                     <  2:740219/149‑1 (MQ=255)
                                                                                                                             cAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                    >  2:1091515/1‑149 (MQ=255)
                                                                                                                               gCTGTAACCGTTCGGGCAAAGCAAAATGCTGTAGTAGTGTGGGTAACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAATGTGGAGGTATCGCGCGTAACGTTTACCTGCTCAACAAGAGAAg                  >  1:1154906/1‑149 (MQ=255)
                                                                                                                                cTGTAACCGTTCGGGAAAAGCAAAATGCTGTTTTTGTGTGGTTTACCGATAACCATAATTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAgg                 <  1:1060499/149‑1 (MQ=255)
                                                                                                                                 tGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc                >  2:4387398/1‑149 (MQ=255)
                                                                                                                                  gTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcg               >  2:4021778/1‑149 (MQ=255)
                                                                                                                                   tAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcgc              <  2:2555579/149‑1 (MQ=255)
                                                                                                                                     aCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            >  1:3806930/1‑149 (MQ=255)
                                                                                                                                     aCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt            >  1:3352392/1‑149 (MQ=255)
                                                                                                                                      ccGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTc           <  1:4312792/149‑1 (MQ=255)
                                                                                                                                      ccGTTCGGGCAAAGAAAATTGCTGTTGTTGTGTGGGTTACCTATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTc           <  1:2911848/149‑1 (MQ=255)
                                                                                                                                         ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGt        >  1:1610244/1‑149 (MQ=255)
                                                                                                                                         ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGt        >  2:3515108/1‑149 (MQ=255)
                                                                                                                                           cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTaa      <  1:4293528/149‑1 (MQ=255)
                                                                                                                                           cGGGAAAAGAAAAATGCTTTTGTTGTGTGGGTTACCTATAACCATCTTTTTGCCCTCACCGAATTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTaa      <  1:4422642/149‑1 (MQ=255)
                                                                                                                                               cAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTa  <  2:4247384/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTA  >  CP009273/3110605‑3110896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGC  >  CP009273/3110608‑3110899
                                                                                                                                               |                                                                                                                                                    
CTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAA                                                                                                                                                >  SRR19973071.3400149/1‑150 (MQ=60)
CTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAA                                                                                                                                                >  SRR19973071.3400489/1‑150 (MQ=60)
 TTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAT                                                                                                                                               <  SRR19973071.4193326/150‑1 (MQ=60)
 TTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAT                                                                                                                                               <  SRR19973071.2762939/150‑1 (MQ=60)
    CGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCT                                                                                                                                            <  SRR19973071.4209074/150‑1 (MQ=60)
    CGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCT                                                                                                                                            <  SRR19973071.4209063/150‑1 (MQ=60)
     GCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTG                                                                                                                                           <  SRR19973071.3504191/150‑1 (MQ=60)
      CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGT                                                                                                                                          <  SRR19973071.3911244/150‑1 (MQ=60)
      CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTAATCCGGGTCGCTGTTTAGCGTAAACAGACCAAAAATATTAAAGCCCCCCTTCCAGCTGTAACCCTTCGGGCAAAGCAAAAATCTGT                                                                                                                                          >  SRR19973071.2869688/1‑150 (MQ=60)
       GTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTT                                                                                                                                         >  SRR19973071.1178657/1‑150 (MQ=60)
           GGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAG                                                                                                                                     >  SRR19973071.2143043/1‑150 (MQ=60)
            GCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGT                                                                                                                                    <  SRR19973071.213657/150‑1 (MQ=60)
             CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                   <  SRR19973071.3512043/150‑1 (MQ=60)
             CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                   <  SRR19973071.1115775/150‑1 (MQ=60)
             CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                   >  SRR19973071.2669384/1‑150 (MQ=60)
             CTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTG                                                                                                                                   <  SRR19973071.3616412/150‑1 (MQ=60)
              TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGT                                                                                                                                  >  SRR19973071.2538344/1‑150 (MQ=60)
              TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGT                                                                                                                                  <  SRR19973071.4688559/150‑1 (MQ=60)
              TTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGT                                                                                                                                  >  SRR19973071.136954/1‑150 (MQ=60)
               TCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTG                                                                                                                                 <  SRR19973071.3716688/150‑1 (MQ=60)
                CCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGG                                                                                                                                >  SRR19973071.12128/1‑150 (MQ=60)
                   TTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTT                                                                                                                             <  SRR19973071.4682751/150‑1 (MQ=60)
                   TTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTT                                                                                                                             <  SRR19973071.2405981/150‑1 (MQ=60)
                    TTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTA                                                                                                                            >  SRR19973071.4521411/1‑150 (MQ=60)
                      ATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCACCTGTAACCGTTAGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACC                                                                                                                          >  SRR19973071.4104420/1‑150 (MQ=60)
                       TCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCG                                                                                                                         <  SRR19973071.875465/150‑1 (MQ=60)
                          TCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATA                                                                                                                      <  SRR19973071.3427983/150‑1 (MQ=60)
                           CGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAA                                                                                                                     <  SRR19973071.4213187/150‑1 (MQ=60)
                            GGNCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCCTCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAC                                                                                                                    >  SRR19973071.4032441/1‑150 (MQ=60)
                            GGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAC                                                                                                                    >  SRR19973071.2920680/1‑150 (MQ=60)
                            GGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATTCTGTTGTAGTGTGGGTTACCGATAAC                                                                                                                    >  SRR19973071.4032225/1‑150 (MQ=60)
                             GACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACC                                                                                                                   >  SRR19973071.445705/1‑150 (MQ=60)
                              ACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCA                                                                                                                  >  SRR19973071.4455545/1‑150 (MQ=60)
                              ACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCA                                                                                                                  <  SRR19973071.263817/150‑1 (MQ=60)
                              ACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCA                                                                                                                  >  SRR19973071.4455561/1‑150 (MQ=60)
                                AGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATC                                                                                                                >  SRR19973071.666558/1‑150 (MQ=60)
                                  ATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAG                                                                                                              <  SRR19973071.1340368/150‑1 (MQ=60)
                                  ATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAG                                                                                                              <  SRR19973071.1340480/150‑1 (MQ=60)
                                  ATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAG                                                                                                              <  SRR19973071.1340522/150‑1 (MQ=60)
                                  ATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAG                                                                                                              <  SRR19973071.221567/150‑1 (MQ=60)
                                    AGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTT                                                                                                            >  SRR19973071.1612755/1‑150 (MQ=60)
                                     GCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTT                                                                                                           <  SRR19973071.74137/150‑1 (MQ=60)
                                         AGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACTATCAGTTTGCCC                                                                                                       <  SRR19973071.2626067/150‑1 (MQ=60)
                                            ACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCA                                                                                                    <  SRR19973071.2257989/150‑1 (MQ=60)
                                            ACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCA                                                                                                    <  SRR19973071.2257986/150‑1 (MQ=60)
                                            ACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCA                                                                                                    <  SRR19973071.3750645/150‑1 (MQ=60)
                                            ACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCA                                                                                                    <  SRR19973071.2257962/150‑1 (MQ=60)
                                              GTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACC                                                                                                  <  SRR19973071.1053074/150‑1 (MQ=60)
                                              GTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACC                                                                                                  >  SRR19973071.2207377/1‑150 (MQ=60)
                                              GTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGGTCCAGCTGTAACCGTTCGGGCAAAGCAAAATTCTGTTGTAGTGTGGGGTACCGATAACCATCAGTTTGCCCTCACC                                                                                                  >  SRR19973071.4086274/1‑150 (MQ=60)
                                               TTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCA                                                                                                 <  SRR19973071.72145/150‑1 (MQ=60)
                                                    CATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAATCAAAATGCTGTTGTAGTGTGGGTTAACGATAACCATCAGTTTGCCCTCACCGACTTG                                                                                            >  SRR19973071.2019028/1‑150 (MQ=60)
                                                      TGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCC                                                                                          <  SRR19973071.2510039/150‑1 (MQ=60)
                                                         AGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCA                                                                                       <  SRR19973071.2822705/150‑1 (MQ=60)
                                                         AGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGCCCCA                                                                                       >  SRR19973071.502569/1‑150 (MQ=60)
                                                             CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                   <  SRR19973071.1093755/150‑1 (MQ=60)
                                                             CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                   <  SRR19973071.1093758/150‑1 (MQ=60)
                                                                CATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAT                                                                                <  SRR19973071.2276832/150‑1 (MQ=60)
                                                                   GTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAA                                                                             <  SRR19973071.4295008/150‑1 (MQ=60)
                                                                   GTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAA                                                                             <  SRR19973071.3655690/150‑1 (MQ=60)
                                                                   GTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAA                                                                             <  SRR19973071.1258216/150‑1 (MQ=60)
                                                                     CATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACG                                                                           >  SRR19973071.2359037/1‑150 (MQ=60)
                                                                     CATCCGGGTCGCTGTTGAGCGTACACAGCCCCTCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTCGGGCAAAGCCAAATTCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTTCCCTCACCGACATGCCCCAGCGAAATAAACG                                                                           >  SRR19973071.492226/1‑150 (MQ=60)
                                                                      ATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGG                                                                          <  SRR19973071.1579956/150‑1 (MQ=60)
                                                                      ATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGG                                                                          <  SRR19973071.3977108/150‑1 (MQ=60)
                                                                       TCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGC                                                                         <  SRR19973071.1729585/150‑1 (MQ=60)
                                                                            GTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTT                                                                    >  SRR19973071.2297078/1‑150 (MQ=60)
                                                                             TCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTA                                                                   >  SRR19973071.1135066/1‑150 (MQ=60)
                                                                                   TTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTG                                                             >  SRR19973071.277216/1‑150 (MQ=60)
                                                                                      AGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCG                                                          <  SRR19973071.195283/150‑1 (MQ=60)
                                                                                      AGCGTACACTGCCCATATTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTAGTGTGGTTTACCGATAACCATAAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCG                                                          <  SRR19973071.2290478/150‑1 (MQ=60)
                                                                                         GTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTA                                                       >  SRR19973071.1581504/1‑150 (MQ=60)
                                                                                         GTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTA                                                       >  SRR19973071.1581785/1‑150 (MQ=60)
                                                                                           ACACTGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTACCCGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCCCCGACTTGCCCCAGCGAAAAACACGGCAGGTTAAAGGTGGCGGTATC                                                     <  SRR19973071.1307626/150‑1 (MQ=60)
                                                                                           ACACGGCCCATCTTTATTACCCCCCCCGTTCCCGCTGTAACCGTTCGGCCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTTTTTGCCCTCACCGACTGGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATC                                                     <  SRR19973071.1307876/150‑1 (MQ=60)
                                                                                            CACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCG                                                    >  SRR19973071.4343357/1‑150 (MQ=60)
                                                                                            CACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCG                                                    >  SRR19973071.4343387/1‑150 (MQ=60)
                                                                                            CACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCG                                                    >  SRR19973071.4343432/1‑150 (MQ=60)
                                                                                            CACGGCCCATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCAGCATTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCG                                                    <  SRR19973071.249884/150‑1 (MQ=60)
                                                                                                    ATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAC                                            >  SRR19973071.4489301/1‑150 (MQ=60)
                                                                                                    ATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAC                                            >  SRR19973071.2290444/1‑150 (MQ=60)
                                                                                                    ATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAC                                            >  SRR19973071.2290474/1‑150 (MQ=60)
                                                                                                     TCTTTATTCACGCCCCCGTTCCAGCTGTACCCGTGGGGGCAAAGAAAATTGCTTTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCCCCGATTTGCCCCCGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACG                                           <  SRR19973071.818345/150‑1 (MQ=60)
                                                                                                      CTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGT                                          >  SRR19973071.4465198/1‑150 (MQ=60)
                                                                                                      CTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGT                                          >  SRR19973071.2947413/1‑150 (MQ=60)
                                                                                                      CTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGT                                          >  SRR19973071.4465994/1‑150 (MQ=60)
                                                                                                          ATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTC                                      >  SRR19973071.810242/1‑150 (MQ=60)
                                                                                                           TTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCC                                     >  SRR19973071.2433594/1‑150 (MQ=60)
                                                                                                           TTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCC                                     >  SRR19973071.1632297/1‑150 (MQ=60)
                                                                                                           TTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCC                                     >  SRR19973071.1632294/1‑150 (MQ=60)
                                                                                                               CGCCCCCGTTCCAGCTGTACCCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGTTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCT                                 <  SRR19973071.4245979/150‑1 (MQ=60)
                                                                                                               CGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCT                                 <  SRR19973071.2592885/150‑1 (MQ=60)
                                                                                                                GCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTC                                >  SRR19973071.3292273/1‑150 (MQ=60)
                                                                                                                    CCTTTCCTGCTGTAACCGTTCGGGAAACGAAAATTGCTGTTTTATTGTGGGTTACCGATAACCATAATTTTGCCCTCACGGAATTGCCCCTGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACA                            <  SRR19973071.3041733/150‑1 (MQ=60)
                                                                                                                         CCAGCTGTAACCGTTCGGGAAAAGAAAAATGCTTTTTTAGTTTGGTTTACCTAAAACAATCTGTTTGCCAACACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGA                       <  SRR19973071.2037860/150‑1 (MQ=60)
                                                                                                                           AGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTAGTAGTGTGGGTAACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAATGTGGAGGTATCGCGCGTAACGTTTACCTGCTCAACAAGAGAAG                     >  SRR19973071.1158917/1‑150 (MQ=60)
                                                                                                                           AGCTGTAACCGTTCGGGCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCGATAACAATCTGTTTGCCCTCACCGACTTGCCCCTGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAG                     <  SRR19973071.174849/150‑1 (MQ=60)
                                                                                                                            GCTGTAACCGTTCGGGAAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGG                    <  SRR19973071.4389935/150‑1 (MQ=60)
                                                                                                                             CTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGC                   <  SRR19973071.1064192/150‑1 (MQ=60)
                                                                                                                             CTGTAACCGTTCGGGAAAAGCAAAATGCTGTTTTTGTGTGGTTTACCGATAACCATAATTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGC                   <  SRR19973071.1064292/150‑1 (MQ=60)
                                                                                                                                TAACCGTTCGGCCAAAGAAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCTGTTTGCCCACACCGACTGGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCT                <  SRR19973071.3332061/150‑1 (MQ=60)
                                                                                                                                 AACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTT               <  SRR19973071.4630390/150‑1 (MQ=60)
                                                                                                                                 AACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTT               >  SRR19973071.3364285/1‑150 (MQ=60)
                                                                                                                                 AACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTT               >  SRR19973071.3820445/1‑150 (MQ=60)
                                                                                                                                   CCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCC             >  SRR19973071.931378/1‑150 (MQ=60)
                                                                                                                                   CCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCC             <  SRR19973071.4328037/150‑1 (MQ=60)
                                                                                                                                   CCGTTCGGGCAAAGAAAATTGCTGTTGTTGTGTGGGTTACCTATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCC             <  SRR19973071.2922282/150‑1 (MQ=60)
                                                                                                                                     GTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGT           >  SRR19973071.1615943/1‑150 (MQ=60)
                                                                                                                                        CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT        <  SRR19973071.4308725/150‑1 (MQ=60)
                                                                                                                                        CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT        >  SRR19973071.2493124/1‑150 (MQ=60)
                                                                                                                                        CGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAT        <  SRR19973071.4308941/150‑1 (MQ=60)
                                                                                                                                         GGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATG       >  SRR19973071.4305285/1‑150 (MQ=60)
                                                                                                                                          GGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT      <  SRR19973071.4301479/150‑1 (MQ=60)
                                                                                                                                          GGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT      <  SRR19973071.3831353/150‑1 (MQ=60)
                                                                                                                                          GGCAAAGAAAATTGCTGTTGTTGTGTGGGTTACCTATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGT      <  SRR19973071.2243681/150‑1 (MQ=60)
                                                                                                                                             AAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG   >  SRR19973071.3479724/1‑150 (MQ=60)
                                                                                                                                             AAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG   >  SRR19973071.2267073/1‑150 (MQ=60)
                                                                                                                                             AAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG   >  SRR19973071.2266990/1‑150 (MQ=60)
                                                                                                                                             AAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG   >  SRR19973071.1872687/1‑150 (MQ=60)
                                                                                                                                              AAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGC  >  SRR19973071.3835947/1‑150 (MQ=60)
                                                                                                                                               |                                                                                                                                                    
CTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGC  >  CP009273/3110608‑3110899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑