Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 66.5% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA66.5% 296.5 / 308.2 286R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (51/45);  new base A (87/103);  total (138/148)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  CP009273/3110604‑3110898
                                                                                                                                                   |                                                                                                                                                   
 tGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCa                                                                                                                                                  >  2:3642804/1‑150 (MQ=255)
 tGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCa                                                                                                                                                  >  2:3545661/1‑150 (MQ=255)
     tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAAt                                                                                                                                              >  2:1610421/1‑150 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTGACCGTTCGGGCAACGCAAAATGCtgt                                                                                                                                         >  2:4488920/1‑150 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTATACATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                         <  1:88130/150‑1 (MQ=255)
           gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCtgtt                                                                                                                                        >  2:2009971/1‑150 (MQ=255)
            tCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgttg                                                                                                                                       <  1:4261261/150‑1 (MQ=255)
            tCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgttg                                                                                                                                       >  1:1734027/1‑150 (MQ=255)
              cGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTa                                                                                                                                     <  1:2805552/150‑1 (MQ=255)
              cGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTa                                                                                                                                     >  2:599509/1‑150 (MQ=255)
               ggCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAg                                                                                                                                    >  2:1761341/1‑150 (MQ=255)
                  ttCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtgt                                                                                                                                 <  2:937070/150‑1 (MQ=255)
                  ttCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtgt                                                                                                                                 <  2:782395/150‑1 (MQ=255)
                  gtCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAgtgt                                                                                                                                 <  2:3755186/149‑1 (MQ=255)
                      aTTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACAGCCCATCTTTAATAACGCCCCCGTTCCAGCAGTAACAGTACGGGCAACGCAAAAATCTGTTGTAGTGTGGGt                                                                                                                             >  1:1430528/1‑150 (MQ=255)
                       tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTAAACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTAGGGCAACGCAAAATGCTGTTGTAGTGTGGGtt                                                                                                                            >  1:2033468/1‑150 (MQ=255)
                           tcgtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCg                                                                                                                        <  1:4688625/150‑1 (MQ=255)
                            cgtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGa                                                                                                                       >  2:1338464/1‑150 (MQ=255)
                              tcgGACATATAGCTCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACAATTCCCATCTTTATTAACGCCCCCGATCCAGCTGTAAACGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGTGTTACAGaaa                                                                                                                     >  2:4000764/1‑148 (MQ=255)
                              tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                     <  1:2336429/150‑1 (MQ=255)
                               cgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATaa                                                                                                                    <  2:4533574/150‑1 (MQ=255)
                                 gACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAAcc                                                                                                                  >  2:3161920/1‑150 (MQ=255)
                                 gACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGGGTGGGTTACCGATAAcc                                                                                                                  >  2:1270446/1‑150 (MQ=255)
                                     gATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCa                                                                                                              >  1:3632028/1‑150 (MQ=255)
                                       tAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGt                                                                                                            <  2:3564054/150‑1 (MQ=255)
                                        aGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAACATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                           <  1:4796616/150‑1 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                         <  1:3899536/150‑1 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                         <  1:4270943/150‑1 (MQ=255)
                                              gcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCt                                                                                                     <  2:3887856/150‑1 (MQ=255)
                                                aCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCa                                                                                                   <  2:4692532/150‑1 (MQ=255)
                                                 cGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAc                                                                                                  <  2:1121929/150‑1 (MQ=255)
                                                 cGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAc                                                                                                  <  2:1745729/150‑1 (MQ=255)
                                                 cGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGGTGTAGTGTGGGTTACCGATAACCATCAGTTTGACCTCAc                                                                                                  >  2:4614533/1‑150 (MQ=255)
                                                  gTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAcc                                                                                                 <  2:3024161/150‑1 (MQ=255)
                                                   ttCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAAAGCTGTTGTAGTGTGGGTTACCGATAACCAACAGTTTGCCCACACCg                                                                                                >  1:3371813/1‑150 (MQ=255)
                                                    tctcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGa                                                                                               >  1:1173286/1‑150 (MQ=255)
                                                    tctcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTAGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGa                                                                                               >  1:136628/1‑150 (MQ=255)
                                                     ctcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGAc                                                                                              >  2:1880488/1‑150 (MQ=255)
                                                     ctcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAGACCCATCAGTTTGCCCTCACCGAc                                                                                              >  2:1484686/1‑150 (MQ=255)
                                                         aTGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGc                                                                                          >  1:3573545/1‑150 (MQ=255)
                                                            aaGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGcccc                                                                                       <  2:1520938/150‑1 (MQ=255)
                                                             aGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCa                                                                                      <  2:290880/150‑1 (MQ=255)
                                                               ttcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGc                                                                                    <  1:2128433/150‑1 (MQ=255)
                                                               ttcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGCCCCAGc                                                                                    >  1:4171894/1‑150 (MQ=255)
                                                               ttcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCACACCCACATGCCCCAGc                                                                                    >  2:4143687/1‑150 (MQ=255)
                                                                tcttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCg                                                                                   <  2:4257872/150‑1 (MQ=255)
                                                                 cttcATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGa                                                                                  >  1:2577086/1‑150 (MQ=255)
                                                                    cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAt                                                                               >  2:3385379/1‑150 (MQ=255)
                                                                     aTGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATa                                                                              <  1:2970897/150‑1 (MQ=255)
                                                                         cATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACg                                                                          <  1:509281/150‑1 (MQ=255)
                                                                          aTCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACgg                                                                         >  2:56411/1‑150 (MQ=255)
                                                                           tCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGc                                                                        <  1:955820/150‑1 (MQ=255)
                                                                             cGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAg                                                                      <  1:3922121/150‑1 (MQ=255)
                                                                              gggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAgg                                                                     <  2:3223232/150‑1 (MQ=255)
                                                                                   gCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaaa                                                                >  2:35936/1‑150 (MQ=255)
                                                                                     tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                              <  2:4757096/150‑1 (MQ=255)
                                                                                        tGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGGTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTgg                                                           <  2:233894/150‑1 (MQ=255)
                                                                                        tGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTgg                                                           <  1:2381974/150‑1 (MQ=255)
                                                                                          aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                         <  2:2663493/150‑1 (MQ=255)
                                                                                            cGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGt                                                       >  1:88829/1‑150 (MQ=255)
                                                                                              tACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTAt                                                     >  2:882521/1‑150 (MQ=255)
                                                                                               acacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATc                                                    >  1:3150152/1‑150 (MQ=255)
                                                                                                cacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcg                                                   >  2:1692139/1‑150 (MQ=255)
                                                                                                  cTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                                 <  2:2403113/150‑1 (MQ=255)
                                                                                                  cTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                                 >  1:1471342/1‑150 (MQ=255)
                                                                                                    gCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcgcg                                               >  1:4777084/1‑150 (MQ=255)
                                                                                                      ccATCTTTATTAACGCCCCCGTTCCAGCTTTAACCTTTCGGGAAACGCAAAATGCTTTTTTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAATTAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTa                                             <  2:609055/150‑1 (MQ=255)
                                                                                                      ccATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGAAAATTGCTGTTGTCGTGTGGGTTACCGATAACCATCAGTTTGCCCTCCCCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTa                                             <  1:4862505/150‑1 (MQ=255)
                                                                                                       cATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTaa                                            >  2:4067858/1‑150 (MQ=255)
                                                                                                       aaTCTTTATTAACGCCCCCGTTCCCGCTGGAACCGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCATTTTGCCCTCACCGACTTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTaa                                            <  2:831623/149‑1 (MQ=255)
                                                                                                          cTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGt                                         >  2:2724854/1‑150 (MQ=255)
                                                                                                          cTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGt                                         >  2:1051273/1‑150 (MQ=255)
                                                                                                             tattaACGCCCCCGTTCCCGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCCGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                      <  2:4524731/150‑1 (MQ=255)
                                                                                                             tattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                      <  2:4460687/150‑1 (MQ=255)
                                                                                                             tataaccGCCCCGGTTCCCGCTGTAACCGTTCGGGCAAAGCAAACTGCTGTTGTTGTGTGGGTTACCTATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                      <  1:1007317/144‑1 (MQ=255)
                                                                                                             tataaccGCCCCCGTTCCCGCTGTAACCGTTGGGCAAACGAAAAATGGTGTTGTTGTTTGGTTTACCTATAACCATCTGTTGGCCCACACCGACTGGCCCCCGCTAAAACCACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                      <  1:1610421/144‑1 (MQ=255)
                                                                                                               ttaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTcc                                    <  2:659905/150‑1 (MQ=255)
                                                                                                                taccGCCCCCTTTCACGATGTAACCGTTGGGTAAACTATAAATGCTTTTTTTGTGTGGGTTACCGATAAACATCATTTTGCCCTCACCGACTTGCCCCCGCGAATTAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                   <  1:4603789/147‑1 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                   >  1:71205/1‑150 (MQ=255)
                                                                                                                   cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGAAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCATTTTGCCCTCACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                                <  2:4483698/150‑1 (MQ=255)
                                                                                                                   cGCCCCCGTACCAGCTGTAACCGTTCGGGCAACGCAAATTGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                                <  2:2029057/150‑1 (MQ=255)
                                                                                                                    gCCCCCTTCCCAGCTGTAACGTTGCGGGAAAAGAAAAATGCTGTTGTTTTGTGGTTTACCGATAACCATCTTTTTGCCCCCACCGACTTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                               <  1:3691960/150‑1 (MQ=255)
                                                                                                                    gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                               <  2:681630/150‑1 (MQ=255)
                                                                                                                    gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                               <  2:1221214/150‑1 (MQ=255)
                                                                                                                       cccGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaac                            >  2:1959410/1‑150 (MQ=255)
                                                                                                                           ttCCTGCTGTAACGGTTGGGGAAAAGAAAAATGCTGTTGTTGTTTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                        <  1:631349/150‑1 (MQ=255)
                                                                                                                           ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                        >  1:4723784/1‑150 (MQ=255)
                                                                                                                           ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                        >  1:4839070/1‑150 (MQ=255)
                                                                                                                           ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                        >  2:3325396/1‑150 (MQ=255)
                                                                                                                           ttCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAaga                        >  1:2662406/1‑150 (MQ=255)
                                                                                                                            tCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                       >  1:875704/1‑150 (MQ=255)
                                                                                                                             ccAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                      >  1:2530216/1‑150 (MQ=255)
                                                                                                                              cAGCTTTAACGGTTCGGTCAAAGAAAAATGCTTTTGTTGTGTGGTTTACCTATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGaa                     <  1:1299801/150‑1 (MQ=255)
                                                                                                                               aGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                    >  1:1949858/1‑150 (MQ=255)
                                                                                                                               aGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                    >  2:4847662/1‑150 (MQ=255)
                                                                                                                               aGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAg                    >  2:4422587/1‑150 (MQ=255)
                                                                                                                                     caCCGGTCGGGCAACGAAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt              <  2:3267970/149‑1 (MQ=255)
                                                                                                                                     aaCCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCtt              >  1:327154/1‑150 (MQ=255)
                                                                                                                                                cAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCAGTAATGTAc   >  2:3003647/1‑150 (MQ=255)
                                                                                                                                                 aaCGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACg  >  1:1768061/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  CP009273/3110604‑3110898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  CP009273/3110602‑3110898
                                                                                                                                                     |                                                                                                                                                   
TGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAA                                                                                                                                                     >  SRR19973081.4660178/1‑150 (MQ=60)
TGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTGTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAA                                                                                                                                                     >  SRR19973081.4660151/1‑150 (MQ=60)
 GCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAG                                                                                                                                                    <  SRR19973081.4744342/150‑1 (MQ=60)
  CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGC                                                                                                                                                   <  SRR19973081.4077761/150‑1 (MQ=60)
    GGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAA                                                                                                                                                 >  SRR19973081.664418/1‑150 (MQ=60)
     GCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAA                                                                                                                                                >  SRR19973081.2229281/1‑150 (MQ=60)
        TTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATG                                                                                                                                             <  SRR19973081.3684291/150‑1 (MQ=60)
            CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGT                                                                                                                                         >  SRR19973081.498405/1‑150 (MQ=60)
            CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGT                                                                                                                                         <  SRR19973081.89119/150‑1 (MQ=60)
            CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGT                                                                                                                                         <  SRR19973081.88887/150‑1 (MQ=60)
            CGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTATACATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGT                                                                                                                                         <  SRR19973081.89084/150‑1 (MQ=60)
             GTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTT                                                                                                                                        >  SRR19973081.4903329/1‑150 (MQ=60)
              TCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTG                                                                                                                                       <  SRR19973081.4287269/150‑1 (MQ=60)
              TCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTG                                                                                                                                       >  SRR19973081.1745409/1‑150 (MQ=60)
              TCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTCGGGCCAAGCAAAATGCTGTTG                                                                                                                                       >  SRR19973081.1744308/1‑150 (MQ=60)
                CGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTA                                                                                                                                     <  SRR19973081.2822624/150‑1 (MQ=60)
                  GCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGT                                                                                                                                   <  SRR19973081.2529904/150‑1 (MQ=60)
                        ATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACAGCCCATCTTTAATAACGCCCCCGTTCCAGCAGTAACAGTACGGGCAACGCAAAAATCTGTTGTAGTGTGGGT                                                                                                                             >  SRR19973081.1440097/1‑150 (MQ=60)
                         TTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGATCGTACACTGCCCATATTTATTAACGCCCCCGTTCCAGCTGTAACCGGTCGGGCAACGCAAAATTCTGTTGTAGTGTGGGTT                                                                                                                            >  SRR19973081.2046188/1‑150 (MQ=60)
                         TTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTAAACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTAGGGCAACGCAAAATGCTGTTGTAGTGTGGGTT                                                                                                                            >  SRR19973081.2046209/1‑150 (MQ=60)
                             TCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCG                                                                                                                        <  SRR19973081.4717054/150‑1 (MQ=60)
                             TCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTCCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCG                                                                                                                        <  SRR19973081.4716896/150‑1 (MQ=60)
                                TCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATA                                                                                                                     <  SRR19973081.2351012/150‑1 (MQ=60)
                                 CGGACAGATAGCGCAGCAAGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAA                                                                                                                    <  SRR19973081.1217084/150‑1 (MQ=60)
                                   GACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACC                                                                                                                  <  SRR19973081.1423597/150‑1 (MQ=60)
                                   GACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACC                                                                                                                  <  SRR19973081.1473930/150‑1 (MQ=60)
                                       GATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCA                                                                                                              <  SRR19973081.1328831/150‑1 (MQ=60)
                                       GATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCA                                                                                                              <  SRR19973081.1328737/150‑1 (MQ=60)
                                       GATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCA                                                                                                              >  SRR19973081.3653965/1‑150 (MQ=60)
                                       GATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCA                                                                                                              <  SRR19973081.2588325/150‑1 (MQ=60)
                                        ATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAG                                                                                                             <  SRR19973081.3357474/150‑1 (MQ=60)
                                         TAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGT                                                                                                            <  SRR19973081.4459108/150‑1 (MQ=60)
                                          AGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAACATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTT                                                                                                           <  SRR19973081.4825527/150‑1 (MQ=60)
                                           GCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTT                                                                                                          <  SRR19973081.3713917/150‑1 (MQ=60)
                                           GCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTT                                                                                                          <  SRR19973081.3125287/150‑1 (MQ=60)
                                            CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTTTGGGTTACCGATAACCATCTGTTTG                                                                                                         <  SRR19973081.3923493/150‑1 (MQ=60)
                                            CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTG                                                                                                         <  SRR19973081.4296989/150‑1 (MQ=60)
                                            CGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTG                                                                                                         <  SRR19973081.3923489/150‑1 (MQ=60)
                                               AGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCC                                                                                                      >  SRR19973081.4040730/1‑150 (MQ=60)
                                               AGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCC                                                                                                      >  SRR19973081.4321675/1‑150 (MQ=60)
                                                   CGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCAC                                                                                                  >  SRR19973081.3175377/1‑150 (MQ=60)
                                                     TTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAAAGCTGTTGTAGTGTGGGTTACCGATAACCAACAGTTTGCCCACACCG                                                                                                >  SRR19973081.3392545/1‑150 (MQ=60)
                                                      TCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGA                                                                                               >  SRR19973081.1180801/1‑150 (MQ=60)
                                                      TCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCCTTCCAGCTGTAACCGTTAGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGA                                                                                               >  SRR19973081.137922/1‑150 (MQ=60)
                                                           ATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGC                                                                                          >  SRR19973081.3595307/1‑150 (MQ=60)
                                                           ATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGC                                                                                          >  SRR19973081.3595580/1‑150 (MQ=60)
                                                            TGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTTCCGATAACCATCAGTTTTCCCTCACCGACTTGCC                                                                                         >  SRR19973081.480893/1‑150 (MQ=60)
                                                               AGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGCCCCA                                                                                      >  SRR19973081.239153/1‑150 (MQ=60)
                                                                 TTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGC                                                                                    <  SRR19973081.2141996/150‑1 (MQ=60)
                                                                 TTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACATGCCCCAGC                                                                                    >  SRR19973081.4197399/1‑150 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                  >  SRR19973081.2593133/1‑150 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                  <  SRR19973081.1468535/150‑1 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGA                                                                                  >  SRR19973081.149029/1‑150 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGGTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGC                                                                                  >  SRR19973081.3457217/1‑150 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACAGCCCCTCGTTATTAACGCCCCCGTTACAGCTGTAAACGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGGTACCGATAACCATCAGTTTTCCCTCACCGACATGCCCCAGCGA                                                                                  >  SRR19973081.424789/1‑150 (MQ=60)
                                                                   CTTCATGTCATCCGGGTCGCTGTTGAGCGTACACAGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTAACGATAACCATCAGTTTGCCCACACCGACTTGCCCCAGCGA                                                                                  >  SRR19973081.2593169/1‑150 (MQ=60)
                                                                      CATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAT                                                                               <  SRR19973081.1269785/150‑1 (MQ=60)
                                                                       ATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATA                                                                              <  SRR19973081.2989200/150‑1 (MQ=60)
                                                                       ATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATA                                                                              <  SRR19973081.463614/150‑1 (MQ=60)
                                                                       ATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGGTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATA                                                                              <  SRR19973081.2912341/150‑1 (MQ=60)
                                                                           CATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCGGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACG                                                                          >  SRR19973081.700543/1‑150 (MQ=60)
                                                                           CATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACG                                                                          <  SRR19973081.512952/150‑1 (MQ=60)
                                                                           CATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACG                                                                          <  SRR19973081.512914/150‑1 (MQ=60)
                                                                             TCCGTGTCTCTGTTGAGCGTACACTGACCATCTTTATTAACGCCCCCTTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAATTAAACGGC                                                                        <  SRR19973081.962224/150‑1 (MQ=60)
                                                                             TCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGC                                                                        <  SRR19973081.962299/150‑1 (MQ=60)
                                                                               CGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAG                                                                      <  SRR19973081.3946179/150‑1 (MQ=60)
                                                                               CGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAG                                                                      <  SRR19973081.1056666/150‑1 (MQ=60)
                                                                                  GTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTT                                                                   >  SRR19973081.4294727/1‑150 (MQ=60)
                                                                                      CTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAG                                                               >  SRR19973081.3650963/1‑150 (MQ=60)
                                                                                      CTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAG                                                               >  SRR19973081.2342930/1‑150 (MQ=60)
                                                                                       TGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGG                                                              >  SRR19973081.1876810/1‑150 (MQ=60)
                                                                                         TTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTG                                                            >  SRR19973081.885126/1‑150 (MQ=60)
                                                                                          TGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTTGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGG                                                           <  SRR19973081.2396713/150‑1 (MQ=60)
                                                                                            agcgtacccgccccatatttataacCGCCCCCGTTCCAGCTGTAACCTTTCGGGAAACCCAAAATGCTTTTTTAGTGTGGGTTACCGATAACAATCTGTTTGCCCACACCGACTTCCCCCAGCCAAAAACACGGCAGGTTAAAGGTGGCG                                                         <  SRR19973081.2109522/125‑1 (MQ=60)
                                                                                            AGCGTACACTGCCCATATTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGAAAAGAAAATTGCTGTTTTTGTGTGGGTTACCTATAACAATCATTTTCCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCG                                                         <  SRR19973081.2109571/150‑1 (MQ=60)
                                                                                             GCGTACACGGCCAATATTTATTAACGCCCCCGTTCCAGCTTTAACCGTTCGGCAAAAGAAAAATGCTTTTGTTGTGTGGGTTACCTATAACAATCAGTTTGCCCACACCGACTTGCCCCAGCGAAATACACGGCAGGTTAAAGGTGGCGG                                                        <  SRR19973081.4816946/150‑1 (MQ=60)
                                                                                              CGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGT                                                       >  SRR19973081.89790/1‑150 (MQ=60)
                                                                                                 ACACTGCCTATCTTTATTAACGCCCCGGTTCAAGCTGTAACGTTTGGGGAAATGAAAATTGCTGTTGTTGTGTGGGTTACCTATAACAATCTGTTTCCCCTCACCTAGTTCCCCCCGCGAAAAACACGGCAGGTTAAAGGTGGCGGTATC                                                    <  SRR19973081.1764125/150‑1 (MQ=31)
                                                                                                 ACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATC                                                    >  SRR19973081.3169378/1‑150 (MQ=60)
                                                                                                 ACACTGCCCATATTTATTAACGCCCCCGTTCCCGCTGTACCCGTTCGGGCAAAGCAAACTGCTGTTGTAGTGTGGGTTACCTATAACCATCTTTTTGCCCCCCCCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATC                                                    <  SRR19973081.3941124/150‑1 (MQ=60)
                                                                                                    CTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCG                                                 >  SRR19973081.1481099/1‑150 (MQ=60)
                                                                                                      GCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                               >  SRR19973081.4806563/1‑150 (MQ=60)
                                                                                                      GCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                               >  SRR19973081.4805931/1‑150 (MQ=60)
                                                                                                      GCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCG                                               >  SRR19973081.4805910/1‑150 (MQ=60)
                                                                                                        CCATCTTTATTACCGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCTATAACCAACAGTTTGCCCTCCCCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTA                                             <  SRR19973081.2389301/150‑1 (MQ=60)
                                                                                                        CCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGAAAATTGCTGTTGTCGTGTGGGTTACCGATAACCATCAGTTTGCCCTCCCCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTA                                             <  SRR19973081.4891669/150‑1 (MQ=60)
                                                                                                               TATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTT                                      >  SRR19973081.3244206/1‑150 (MQ=60)
                                                                                                               TATAACCGCCCCGGTTCCCGCTGTAACCGTTCGGGCAAAGCAAACTGCTGTTGTTGTGTGGGTTACCTATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTT                                      <  SRR19973081.1014148/150‑1 (MQ=60)
                                                                                                               TATAACCGCCCCCGTTCCCGCTGTAACCGTTGGGCAAACGAAAAATGGTGTTGTTGTTTGGTTTACCTATAACCATCTGTTGGCCCACACCGACTGGCCCCCGCTAAAACCACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTT                                      <  SRR19973081.1620789/150‑1 (MQ=36)
                                                                                                                ATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTC                                     >  SRR19973081.2761561/1‑150 (MQ=60)
                                                                                                                ATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTC                                     >  SRR19973081.2761525/1‑150 (MQ=60)
                                                                                                                 TTAACGCCCCCGTTCCTGCTGTAACCGTTCGGGCAAAGCAAATTGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCC                                    <  SRR19973081.2401350/150‑1 (MQ=60)
                                                                                                                  TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                   >  SRR19973081.72014/1‑150 (MQ=60)
                                                                                                                  TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                   >  SRR19973081.4857830/1‑150 (MQ=60)
                                                                                                                  TAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCG                                   >  SRR19973081.4857541/1‑150 (MQ=60)
                                                                                                                        CCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAA                             >  SRR19973081.3390506/1‑150 (MQ=60)
                                                                                                                            GTTCCAGATGTAACCGTTCGGGAAAATAAAATTGTTGTTTTAGTGTGGTTTACCTAAAACCATCATTTTGCCCACACCGACTTGCCCAAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAG                         <  SRR19973081.4200240/150‑1 (MQ=60)
                                                                                                                             TTCCTGCTGTAACGGTTGGGGAAAAGAAAAATGCTGTTGTTGTTTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        <  SRR19973081.635732/150‑1 (MQ=60)
                                                                                                                             TTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        >  SRR19973081.2678894/1‑150 (MQ=60)
                                                                                                                             TTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        >  SRR19973081.4868147/1‑150 (MQ=60)
                                                                                                                             TTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        >  SRR19973081.4870091/1‑150 (MQ=60)
                                                                                                                             TTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        >  SRR19973081.2678935/1‑150 (MQ=60)
                                                                                                                             TTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGA                        >  SRR19973081.4752383/1‑150 (MQ=60)
                                                                                                                              TCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAG                       >  SRR19973081.881382/1‑150 (MQ=60)
                                                                                                                              TCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAG                       >  SRR19973081.881501/1‑150 (MQ=60)
                                                                                                                               CCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGA                      >  SRR19973081.2545997/1‑150 (MQ=60)
                                                                                                                               CCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGA                      >  SRR19973081.609021/1‑150 (MQ=60)
                                                                                                                                CAGCTTTAACGGTTCGGTCAAAGAAAAATGCTTTTGTTGTGTGGTTTACCTATAACAATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATACACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAA                     <  SRR19973081.1308484/150‑1 (MQ=60)
                                                                                                                                CAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAC                     >  SRR19973081.4737655/1‑150 (MQ=60)
                                                                                                                                 AGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAG                    >  SRR19973081.1962047/1‑150 (MQ=60)
                                                                                                                                 AGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAG                    >  SRR19973081.1962195/1‑150 (MQ=60)
                                                                                                                                    TGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCG                 >  SRR19973081.3885469/1‑150 (MQ=60)
                                                                                                                                    TGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCG                 >  SRR19973081.1409816/1‑150 (MQ=60)
                                                                                                                                     GTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGC                >  SRR19973081.4733364/1‑150 (MQ=60)
                                                                                                                                     GTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGC                >  SRR19973081.1554439/1‑150 (MQ=60)
                                                                                                                                       AACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTT              >  SRR19973081.329502/1‑150 (MQ=60)
                                                                                                                                                  CAACGCAAAATGCTTTTGTTGTGTGGTTTACCTATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAC   <  SRR19973081.2962263/150‑1 (MQ=60)
                                                                                                                                                  CAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAC   <  SRR19973081.2007567/150‑1 (MQ=60)
                                                                                                                                                  CAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCTGTTTGCCCACACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAC   <  SRR19973081.792919/150‑1 (MQ=60)
                                                                                                                                                   AACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  SRR19973081.1779321/1‑150 (MQ=60)
                                                                                                                                                   AACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  SRR19973081.1779630/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACG  >  CP009273/3110602‑3110898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑