Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 542,200 Δ1 bp 88.7% coding (65/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273542,2000G.88.7% 742.2 / 94.8 177coding (65/1668 nt)fdrAputative NAD(P)‑binding acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (9/10);  new base . (101/56);  total (111/66)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 183 total aligned reads displayed.

TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAG  >  CP009273/542053‑542344
                                                                                                                                                   |                                                                                                                                                
tttACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaa                                                                                                                                                <  2:1106378/150‑1 (MQ=255)
  tACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaaac                                                                                                                                              <  1:3190620/150‑2 (MQ=255)
  tACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaaac                                                                                                                                              <  1:1955200/150‑2 (MQ=255)
      cGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATTATTATTTCAC‑AAAACTCAg                                                                                                                                         <  2:4595696/150‑1 (MQ=255)
       ggtGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGc                                                                                                                                        <  1:2921988/148‑1 (MQ=255)
       gTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGc                                                                                                                                        >  1:3733991/1‑150 (MQ=255)
        ttGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCg                                                                                                                                       <  2:1176152/150‑1 (MQ=255)
          gTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGaa                                                                                                                                     >  1:1956125/1‑150 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                  >  2:1411556/1‑150 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                  <  1:1802295/150‑1 (MQ=255)
               cGATACCGTAATCGCCACATTAACAATGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCTGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGa                                                                                                                                <  2:2521681/150‑1 (MQ=255)
                gATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGa                                                                                                                                >  2:354087/1‑150 (MQ=255)
                gATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGaa                                                                                                                               >  1:3024854/1‑150 (MQ=255)
                   aCCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAt                                                                                                                            <  1:188756/150‑1 (MQ=255)
                   aCCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAt                                                                                                                            >  1:5071231/1‑150 (MQ=255)
                    ccGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATg                                                                                                                           >  2:4266736/1‑150 (MQ=255)
                     cGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGt                                                                                                                          >  1:2691914/1‑150 (MQ=255)
                       tAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtg                                                                                                                        >  1:374960/1‑150 (MQ=255)
                       tAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtg                                                                                                                        >  1:370984/1‑150 (MQ=255)
                       tAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGCTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtg                                                                                                                        >  1:4999282/1‑150 (MQ=255)
                           cGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtgatga                                                                                                                    >  2:782410/1‑150 (MQ=255)
                            gCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTgatgat                                                                                                                   <  2:1480948/150‑1 (MQ=255)
                              cacaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGt                                                                                                                 >  2:2753826/1‑150 (MQ=255)
                               acaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGt                                                                                                                 >  1:2455527/1‑150 (MQ=255)
                               acaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGtt                                                                                                                >  1:315884/1‑150 (MQ=255)
                                caTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGttt                                                                                                               <  2:3075857/150‑1 (MQ=255)
                                caTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGttt                                                                                                               <  1:2121316/150‑1 (MQ=255)
                                caTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGttt                                                                                                               <  2:3076353/150‑1 (MQ=255)
                                caTTAACACTGCTCGTGCAATGGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGttt                                                                                                               <  2:2866081/150‑1 (MQ=255)
                                 aTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGttt                                                                                                               >  1:1177343/1‑150 (MQ=255)
                                 aTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTc                                                                                                              <  2:2447854/150‑1 (MQ=255)
                                 aTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTc                                                                                                              <  2:2302529/150‑1 (MQ=255)
                                 aTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTc                                                                                                              >  1:1677859/1‑150 (MQ=255)
                                         tGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATTATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatga                                                                                                      >  2:3577264/1‑150 (MQ=255)
                                         tGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatga                                                                                                      >  1:4179553/1‑150 (MQ=255)
                                           cTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatgatg                                                                                                    <  2:4927554/150‑1 (MQ=255)
                                               tGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTa                                                                                                <  2:717978/150‑1 (MQ=255)
                                               tGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTa                                                                                                >  1:3812373/1‑150 (MQ=255)
                                                gCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTAAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAc                                                                                               <  1:4428557/150‑1 (MQ=255)
                                                 cAATTGCCAGGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcg                                                                                              <  1:1098416/150‑1 (MQ=255)
                                                  aaTTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcgc                                                                                             >  1:4220962/1‑150 (MQ=255)
                                                    ttGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcgccc                                                                                           <  2:850423/150‑1 (MQ=255)
                                                       ccATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcgcccgcc                                                                                        >  1:1003552/1‑150 (MQ=255)
                                                         aTGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCaa                                                                                      >  2:4193873/1‑150 (MQ=255)
                                                          tGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAAt                                                                                     <  2:2086912/150‑1 (MQ=255)
                                                           gggTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATa                                                                                    >  2:3491931/1‑150 (MQ=255)
                                                            ggTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATaa                                                                                   >  2:2055150/1‑150 (MQ=255)
                                                            ggTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATaa                                                                                   >  1:3178420/1‑150 (MQ=255)
                                                                 aaTTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGt                                                                              >  2:3351029/1‑150 (MQ=255)
                                                                     tttAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGttat                                                                           <  1:1796877/150‑1 (MQ=255)
                                                                      ttAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGttatt                                                                          <  1:3135109/150‑1 (MQ=255)
                                                                       tAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAg                                                                        >  1:4378939/1‑150 (MQ=255)
                                                                        aaGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGa                                                                       >  2:1503937/1‑150 (MQ=255)
                                                                              ttgttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcac                                                                 <  2:3123525/150‑1 (MQ=255)
                                                                               tgttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcac                                                                 <  1:879165/150‑1 (MQ=255)
                                                                               tgttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcac                                                                 <  1:879136/150‑1 (MQ=255)
                                                                                  tATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGt                                                             >  2:1102862/1‑150 (MQ=255)
                                                                                   aTGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGtt                                                            <  2:1308341/150‑1 (MQ=255)
                                                                                    tGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGttt                                                           >  1:872116/1‑150 (MQ=255)
                                                                                    tGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGttt                                                           >  1:769626/1‑150 (MQ=255)
                                                                                    tGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGttt                                                           >  1:3475496/1‑150 (MQ=255)
                                                                                       tCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTg                                                        >  1:1387215/1‑150 (MQ=255)
                                                                                           cGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCAt                                                    <  2:378831/150‑1 (MQ=255)
                                                                                           cGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCAt                                                    >  2:1858640/1‑150 (MQ=255)
                                                                                            gCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATg                                                   >  1:3789697/1‑150 (MQ=255)
                                                                                              cTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGAc                                                 <  2:3970176/150‑1 (MQ=255)
                                                                                                ttattaAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGa                                               >  1:277508/1‑150 (MQ=255)
                                                                                                 tattaAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAATCTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGa                                               <  2:2236604/150‑1 (MQ=255)
                                                                                                 tattaAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGAt                                              >  2:4957548/1‑150 (MQ=255)
                                                                                                   ttaAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGAttt                                            <  2:357253/150‑1 (MQ=255)
                                                                                                    taAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGAtttt                                           >  1:5087842/1‑150 (MQ=255)
                                                                                                      aaaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaa                                         >  2:5082517/1‑150 (MQ=255)
                                                                                                       aaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaat                                        >  2:558208/1‑150 (MQ=255)
                                                                                                        aaaGGGAGTTTTCAGGATTCGGTCATTTTAATTATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaata                                       <  2:898628/150‑1 (MQ=255)
                                                                                                                  ttCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacgcc                             >  1:1635178/1‑150 (MQ=255)
                                                                                                                  ttCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacgcc                             >  1:1574646/1‑150 (MQ=255)
                                                                                                                   tCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCg                            >  2:4525536/1‑150 (MQ=255)
                                                                                                                    cAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                           <  1:1202003/150‑1 (MQ=255)
                                                                                                                    cAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                           >  2:632018/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                           >  1:2512935/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGaa                          >  2:1715075/1‑150 (MQ=255)
                                                                                                                      ggATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAAc                         >  1:385792/1‑150 (MQ=255)
                                                                                                                        aTTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGa                       >  2:1466152/1‑150 (MQ=255)
                                                                                                                          tCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGata                     <  2:2902543/150‑1 (MQ=255)
                                                                                                                          tCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATATTAATAACGCCACGCCGAACGata                     <  1:1840021/150‑1 (MQ=255)
                                                                                                                               cAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGc                >  2:2392393/1‑150 (MQ=255)
                                                                                                                                aGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg               >  1:3960633/1‑150 (MQ=255)
                                                                                                                                aGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg               >  2:845851/1‑150 (MQ=255)
                                                                                                                                aGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg               >  1:2804355/1‑150 (MQ=255)
                                                                                                                                aGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg               <  2:3733991/150‑1 (MQ=255)
                                                                                                                                  tttAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAGGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTg             >  2:705065/1‑150 (MQ=255)
                                                                                                                                       tGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAAt        <  2:1047335/150‑1 (MQ=255)
                                                                                                                                        gATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAAtt       >  2:5033875/1‑150 (MQ=255)
                                                                                                                                          ttattTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTc      >  1:1867597/1‑150 (MQ=255)
                                                                                                                                          ttattTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCg     >  2:4848451/1‑150 (MQ=255)
                                                                                                                                          ttattTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCg     >  1:4946427/1‑150 (MQ=255)
                                                                                                                                           tattTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATGCGt    >  1:4709679/1‑150 (MQ=255)
                                                                                                                                            attTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCACTTCGTa   >  2:5058517/1‑150 (MQ=255)
                                                                                                                                             tttCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTa   >  2:1832307/1‑150 (MQ=255)
                                                                                                                                             tttCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAg  >  2:386042/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAG  >  CP009273/542053‑542344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGT  >  CP009273/542055‑542342
                                                                                                                                                 |                                                                                                                                              
TACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAAC                                                                                                                                           <  SRR19973083.1965197/150‑1 (MQ=60)
TACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAAC                                                                                                                                           <  SRR19973083.4117134/150‑1 (MQ=60)
TACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAAC                                                                                                                                           <  SRR19973083.3205799/150‑1 (MQ=60)
TACTCGGTGTCATCGAGACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAAC                                                                                                                                           <  SRR19973083.1965209/150‑1 (MQ=60)
     GTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGC                                                                                                                                      >  SRR19973083.3750939/1‑150 (MQ=60)
     GTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGC                                                                                                                                      >  SRR19973083.1284480/1‑150 (MQ=60)
     GGTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGC                                                                                                                                      <  SRR19973083.2936283/150‑1 (MQ=60)
        GTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAA                                                                                                                                   >  SRR19973083.1966123/1‑150 (MQ=60)
           ATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCA                                                                                                                                >  SRR19973083.4399296/1‑150 (MQ=60)
           ATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCA                                                                                                                                <  SRR19973083.3904695/150‑1 (MQ=60)
           ATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCA                                                                                                                                <  SRR19973083.1810853/150‑1 (MQ=60)
           ATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCA                                                                                                                                <  SRR19973083.1811329/150‑1 (MQ=60)
            TCGATACCGTAATCGCCACATTAACACTGCTCGTGCAAGTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAG                                                                                                                               <  SRR19973083.3201248/150‑1 (MQ=60)
              GATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAA                                                                                                                             >  SRR19973083.3039410/1‑150 (MQ=60)
               ATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAA                                                                                                                            >  SRR19973083.1662905/1‑150 (MQ=60)
                 ACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAT                                                                                                                          >  SRR19973083.2503872/1‑150 (MQ=60)
                 ACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAT                                                                                                                          <  SRR19973083.189822/150‑1 (MQ=60)
                 ACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAT                                                                                                                          >  SRR19973083.5092927/1‑150 (MQ=60)
                   CGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGT                                                                                                                        >  SRR19973083.2705405/1‑150 (MQ=60)
                     TAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTG                                                                                                                      >  SRR19973083.376709/1‑150 (MQ=60)
                     TAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTG                                                                                                                      >  SRR19973083.372719/1‑150 (MQ=60)
                     TAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGCTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTG                                                                                                                      >  SRR19973083.5020745/1‑150 (MQ=60)
                        TCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATG                                                                                                                   <  SRR19973083.3136806/150‑1 (MQ=60)
                             ACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGT                                                                                                               >  SRR19973083.2468107/1‑150 (MQ=60)
                             ACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTT                                                                                                              >  SRR19973083.2943398/1‑150 (MQ=60)
                             ACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTT                                                                                                              >  SRR19973083.317531/1‑150 (MQ=60)
                             ACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTT                                                                                                              >  SRR19973083.317482/1‑150 (MQ=60)
                              CATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTT                                                                                                             <  SRR19973083.2132015/150‑1 (MQ=60)
                              CATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTT                                                                                                             <  SRR19973083.2815150/150‑1 (MQ=60)
                               ATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTT                                                                                                             >  SRR19973083.1183192/1‑150 (MQ=60)
                               ATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTC                                                                                                            >  SRR19973083.1686157/1‑150 (MQ=60)
                                     ACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCTTAAT                                                                                                      >  SRR19973083.2924979/1‑150 (MQ=60)
                                      CTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATG                                                                                                     <  SRR19973083.1919363/150‑1 (MQ=60)
                                       TGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGA                                                                                                    >  SRR19973083.4197959/1‑150 (MQ=60)
                                         CTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATG                                                                                                  >  SRR19973083.4985726/1‑150 (MQ=60)
                                            GTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGT                                                                                               <  SRR19973083.2336423/150‑1 (MQ=60)
                                             TGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTA                                                                                              >  SRR19973083.3829517/1‑150 (MQ=60)
                                             TGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTA                                                                                              >  SRR19973083.3829457/1‑150 (MQ=60)
                                              GCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTAAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAC                                                                                             <  SRR19973083.1846259/150‑1 (MQ=60)
                                              GCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTAAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAC                                                                                             <  SRR19973083.4447822/150‑1 (MQ=60)
                                               CAATTGCCAGGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACG                                                                                            <  SRR19973083.1103945/150‑1 (MQ=60)
                                                AATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGC                                                                                           >  SRR19973083.4239473/1‑150 (MQ=60)
                                                  TTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCC                                                                                         >  SRR19973083.3738849/1‑150 (MQ=60)
                                                   TGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGaaaataatc                                                                                        >  SRR19973083.912543/1‑141 (MQ=60)
                                                   TGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAAATCAGCGAATCAGAAAATGTTGATGATGTTACCGTAATGATGGGTACGCCCG                                                                                        >  SRR19973083.1176042/1‑150 (MQ=60)
                                                     CCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCC                                                                                      >  SRR19973083.1008579/1‑150 (MQ=60)
                                                          GGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAA                                                                                 >  SRR19973083.3193563/1‑150 (MQ=60)
                                                                   TTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTAT                                                                         <  SRR19973083.1805870/150‑1 (MQ=60)
                                                                   TTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTAT                                                                         <  SRR19973083.3509342/150‑1 (MQ=60)
                                                                    TTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATT                                                                        <  SRR19973083.3150100/150‑1 (MQ=60)
                                                                     TAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAG                                                                      >  SRR19973083.4397968/1‑150 (MQ=60)
                                                                      AAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGA                                                                     <  SRR19973083.4761817/150‑1 (MQ=60)
                                                                             TGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCAC                                                               <  SRR19973083.883283/150‑1 (MQ=60)
                                                                             TGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCAC                                                               <  SRR19973083.883203/150‑1 (MQ=60)
                                                                             TGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCAC                                                               <  SRR19973083.883262/150‑1 (MQ=60)
                                                                             TGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCAC                                                               <  SRR19973083.883253/150‑1 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.3491378/1‑150 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.774163/1‑150 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.773390/1‑150 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.773347/1‑150 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.876184/1‑150 (MQ=60)
                                                                                  TGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTT                                                         >  SRR19973083.3491186/1‑150 (MQ=60)
                                                                                     TCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTG                                                      >  SRR19973083.1394153/1‑150 (MQ=60)
                                                                                     TCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTG                                                      >  SRR19973083.4358713/1‑150 (MQ=60)
                                                                                     TCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTG                                                      >  SRR19973083.1394138/1‑150 (MQ=60)
                                                                                          GCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATG                                                 >  SRR19973083.3806783/1‑150 (MQ=60)
                                                                                           CCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGA                                                <  SRR19973083.4492581/150‑1 (MQ=60)
                                                                                              TTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGA                                             >  SRR19973083.278969/1‑150 (MQ=60)
                                                                                                  TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                         >  SRR19973083.5109568/1‑150 (MQ=60)
                                                                                                  TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                         >  SRR19973083.5109576/1‑150 (MQ=60)
                                                                                                  TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                         >  SRR19973083.1188528/1‑150 (MQ=60)
                                                                                                  TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                         >  SRR19973083.3795711/1‑150 (MQ=60)
                                                                                                          GGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGC                                 >  SRR19973083.1983011/1‑150 (MQ=60)
                                                                                                                TTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCC                           >  SRR19973083.1643325/1‑150 (MQ=60)
                                                                                                                TTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCC                           >  SRR19973083.1582549/1‑150 (MQ=60)
                                                                                                                  CAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGA                         <  SRR19973083.1207943/150‑1 (MQ=60)
                                                                                                                   AGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGA                         >  SRR19973083.2525724/1‑150 (MQ=60)
                                                                                                                    GGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAAC                       >  SRR19973083.387576/1‑150 (MQ=60)
                                                                                                                    GGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAAC                       >  SRR19973083.387228/1‑150 (MQ=60)
                                                                                                                     GATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACG                      <  SRR19973083.2778990/150‑1 (MQ=60)
                                                                                                                        TCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATATTAATAACGCCACGCCGAACGATA                   <  SRR19973083.1849296/150‑1 (MQ=60)
                                                                                                                              AGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCG             >  SRR19973083.2818267/1‑150 (MQ=60)
                                                                                                                              AGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCG             >  SRR19973083.3978271/1‑150 (MQ=60)
                                                                                                                                 TTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGG          >  SRR19973083.1852732/1‑150 (MQ=60)
                                                                                                                                       ATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTC    >  SRR19973083.1719511/1‑150 (MQ=60)
                                                                                                                                        TTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTC    >  SRR19973083.1877040/1‑150 (MQ=60)
                                                                                                                                        TTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCG   >  SRR19973083.4967689/1‑150 (MQ=60)
                                                                                                                                         TATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATGCGT  >  SRR19973083.4729839/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                              
TACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGT  >  CP009273/542055‑542342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑