Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 542,200 Δ1 bp 72.1% coding (65/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273542,2000G.72.1% 377.3 / 266.0 172coding (65/1668 nt)fdrAputative NAD(P)‑binding acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (37/11);  new base . (88/36);  total (125/47)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAA  >  CP009273/542053‑542348
                                                                                                                                                   |                                                                                                                                                    
tttACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaa                                                                                                                                                    >  1:4243841/1‑150 (MQ=255)
tttACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaa                                                                                                                                                    >  1:4243870/1‑150 (MQ=255)
tttACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACaaa                                                                                                                                                    >  2:1539734/1‑150 (MQ=255)
     tCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCa                                                                                                                                              >  2:808668/1‑150 (MQ=255)
         tGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGa                                                                                                                                          >  2:151281/1‑150 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                      >  2:945489/1‑150 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                      <  2:658797/150‑1 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                      <  1:1506094/150‑1 (MQ=255)
             aTCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCa                                                                                                                                      >  2:3388393/1‑150 (MQ=255)
              tCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAg                                                                                                                                     <  1:4350181/150‑1 (MQ=255)
                  tACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGAGAATCAGaaa                                                                                                                                  >  2:2587250/1‑150 (MQ=255)
                   aCCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAt                                                                                                                                <  2:105692/150‑1 (MQ=255)
                       tAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtg                                                                                                                            >  1:4673282/1‑150 (MQ=255)
                       tAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtg                                                                                                                            >  1:4672463/1‑150 (MQ=255)
                          tCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCTGTCAGATTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTtgatg                                                                                                                         >  1:2820221/1‑150 (MQ=255)
                          tCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGACAATGTtgatg                                                                                                                         >  2:4677437/1‑150 (MQ=255)
                              cacaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAACATGTTGATGATGt                                                                                                                     >  2:656557/1‑150 (MQ=255)
                              cacaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGt                                                                                                                     <  1:1010931/150‑1 (MQ=255)
                              cacaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGt                                                                                                                     >  2:2718190/1‑150 (MQ=255)
                              cacaTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGt                                                                                                                     <  1:1010801/150‑1 (MQ=255)
                                 aTTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTc                                                                                                                  >  1:4871828/1‑150 (MQ=255)
                                  ttAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTcc                                                                                                                 <  1:640801/150‑1 (MQ=255)
                                    aaCACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCg                                                                                                                >  1:4394180/1‑150 (MQ=255)
                                    aaCACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCg                                                                                                                >  1:3568838/1‑150 (MQ=255)
                                       acTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAat                                                                                                            >  2:1937336/1‑150 (MQ=255)
                                        cTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatg                                                                                                           >  2:3986453/1‑150 (MQ=255)
                                        cTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatg                                                                                                           <  1:2047153/150‑1 (MQ=255)
                                        cTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAatg                                                                                                           >  1:2431690/1‑150 (MQ=255)
                                            tCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATTATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATgg                                                                                                       >  1:1062074/1‑150 (MQ=255)
                                              gTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGt                                                                                                     <  1:2658672/150‑1 (MQ=255)
                                                 cAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcg                                                                                                  >  1:453678/1‑150 (MQ=255)
                                                      gCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTAcgcccg                                                                                              >  1:2899609/1‑150 (MQ=255)
                                                        cATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGTTGTTTCCGTAATGATGGGTACGCCCGCCa                                                                                           >  2:3893547/1‑150 (MQ=255)
                                                        cATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCa                                                                                           <  2:439665/150‑1 (MQ=255)
                                                        cATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCa                                                                                           >  2:4301039/1‑150 (MQ=255)
                                                         aTGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCaa                                                                                          >  2:2367895/1‑150 (MQ=255)
                                                         aTGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCaa                                                                                          >  1:1859148/1‑150 (MQ=255)
                                                          tGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCaa                                                                                          <  1:256956/150‑1 (MQ=255)
                                                          tGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAAt                                                                                         >  2:2509431/1‑150 (MQ=255)
                                                           gggTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAAt                                                                                         >  2:2811109/1‑150 (MQ=255)
                                                              tGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATaaa                                                                                      >  1:3793387/1‑150 (MQ=255)
                                                              tGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAg                                                                                     <  1:1539680/150‑1 (MQ=255)
                                                              tGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAg                                                                                     <  1:1540945/150‑1 (MQ=255)
                                                               gCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAg                                                                                     >  1:656082/1‑150 (MQ=255)
                                                                  aTTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGtt                                                                                 >  1:2602455/1‑150 (MQ=255)
                                                                  aTTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGtt                                                                                 >  1:171326/1‑150 (MQ=255)
                                                                      ttAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGttatt                                                                              >  2:2926452/1‑150 (MQ=255)
                                                                          ggAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATa                                                                         >  1:531219/1‑150 (MQ=255)
                                                                          ggAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATa                                                                         <  2:2383057/150‑1 (MQ=255)
                                                                               tgttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcaca                                                                    >  2:4400705/1‑150 (MQ=255)
                                                                               tgttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACcaca                                                                    >  2:2511184/1‑150 (MQ=255)
                                                                                 ttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAgg                                                                  >  1:4096511/1‑150 (MQ=255)
                                                                                 ttATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAgg                                                                  >  1:3692743/1‑150 (MQ=255)
                                                                                  tATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGt                                                                 >  1:1265198/1‑150 (MQ=255)
                                                                                      aTCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCt                                                             >  2:1091677/1‑150 (MQ=255)
                                                                                       tCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTg                                                            <  1:308387/150‑1 (MQ=255)
                                                                                         cACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTgg                                                           >  2:4508562/1‑150 (MQ=255)
                                                                                         cACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTgg                                                           <  2:1628695/150‑1 (MQ=255)
                                                                                         cACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTgg                                                           <  2:1630656/150‑1 (MQ=255)
                                                                                           cGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCa                                                         >  2:1460342/1‑150 (MQ=255)
                                                                                                   ttaAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGAttt                                                >  2:787179/1‑150 (MQ=255)
                                                                                                    taAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGAtttt                                               <  1:2224577/150‑1 (MQ=255)
                                                                                                       aaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaa                                             >  2:2615157/1‑150 (MQ=255)
                                                                                                       aaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaa                                             <  2:3819783/150‑1 (MQ=255)
                                                                                                       aaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaat                                            >  2:207605/1‑150 (MQ=255)
                                                                                                        aaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTATATACCACAGGTTTCTGGCATGACGATTTtaata                                           >  1:2139197/1‑150 (MQ=255)
                                                                                                        aaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaata                                           >  1:3835364/1‑150 (MQ=255)
                                                                                                        aaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTtaata                                           >  1:1373473/1‑150 (MQ=255)
                                                                                                           gggTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacg                                        <  2:1944489/150‑1 (MQ=255)
                                                                                                           gggTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacg                                        <  1:2281578/150‑1 (MQ=255)
                                                                                                            ggTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacg                                        >  1:2992440/1‑150 (MQ=255)
                                                                                                                  ttCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacgcc                                 >  2:2697573/1‑150 (MQ=255)
                                                                                                                  ttCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacgcc                                 >  2:1004048/1‑150 (MQ=255)
                                                                                                                  ttCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAAGGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacgcc                                 >  2:2697529/1‑150 (MQ=255)
                                                                                                                    cAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                               >  2:2276290/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAGTGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                               >  2:2754036/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                               >  2:2753865/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                               >  1:2742792/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGa                               >  1:2744093/1‑150 (MQ=255)
                                                                                                                     aGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGaa                              >  2:1557308/1‑150 (MQ=255)
                                                                                                                       gATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACg                            <  2:907422/150‑1 (MQ=255)
                                                                                                                           cGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGatat                        <  1:926683/150‑1 (MQ=255)
                                                                                                                            ggTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGatat                        >  2:2171026/1‑150 (MQ=255)
                                                                                                                            ggTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGatat                        >  2:1213989/1‑150 (MQ=255)
                                                                                                                            ggTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGatat                        >  2:1489103/1‑150 (MQ=255)
                                                                                                                             gTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATAttt                      <  2:1257428/150‑1 (MQ=255)
                                                                                                                             gTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATAttt                      <  2:1257429/150‑1 (MQ=255)
                                                                                                                               cAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGc                    >  1:206021/1‑150 (MQ=255)
                                                                                                                                 gTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg                   >  1:1828956/1‑150 (MQ=255)
                                                                                                                                 gTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCg                   <  1:3370598/150‑1 (MQ=255)
                                                                                                                                 gTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGt                  >  2:1376444/1‑150 (MQ=255)
                                                                                                                                    tAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTGATAACGCCACGCCGAACGATATTTGCGTgg                >  2:1111001/1‑150 (MQ=255)
                                                                                                                                    tAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTgg                >  2:3250234/1‑150 (MQ=255)
                                                                                                                                    tAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTgg                >  2:3250230/1‑150 (MQ=255)
                                                                                                                                    tAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGc               >  2:717559/1‑150 (MQ=255)
                                                                                                                                    tAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGc               >  2:3150238/1‑150 (MQ=255)
                                                                                                                                      aTGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATTTTTCCTTAATGATGGGTACGCCCGCCAATAAAGCGTTAATAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGAGTGGCaa             >  1:812492/1‑150 (MQ=255)
                                                                                                                                      aTGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCaa             >  2:3039254/1‑150 (MQ=255)
                                                                                                                                            attTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTa       >  1:3140563/1‑150 (MQ=255)
                                                                                                                                                  cGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGaa  >  1:2228913/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAA  >  CP009273/542053‑542348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAA  >  CP009273/542053‑542348
                                                                                                                                                   |                                                                                                                                                    
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACAAA                                                                                                                                                    >  SRR19973081.4269760/1‑150 (MQ=60)
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACAAA                                                                                                                                                    >  SRR19973081.4269789/1‑150 (MQ=60)
        TTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCG                                                                                                                                           >  SRR19973081.3069657/1‑150 (MQ=60)
          GTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAA                                                                                                                                         >  SRR19973081.2070766/1‑150 (MQ=60)
           TCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAAT                                                                                                                                        >  SRR19973081.3001847/1‑150 (MQ=60)
             ATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCA                                                                                                                                      <  SRR19973081.1516024/150‑1 (MQ=60)
              TCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAG                                                                                                                                     <  SRR19973081.4376562/150‑1 (MQ=60)
              TCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAG                                                                                                                                     <  SRR19973081.4378944/150‑1 (MQ=60)
                       TAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTG                                                                                                                            >  SRR19973081.4701642/1‑150 (MQ=60)
                       TAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTG                                                                                                                            >  SRR19973081.4700819/1‑150 (MQ=60)
                          TCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCTGTCAGATTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATG                                                                                                                         >  SRR19973081.2837349/1‑150 (MQ=60)
                              CACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGT                                                                                                                     <  SRR19973081.1017779/150‑1 (MQ=60)
                              CACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGT                                                                                                                     <  SRR19973081.1017649/150‑1 (MQ=60)
                                 ATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTC                                                                                                                  >  SRR19973081.4901026/1‑150 (MQ=60)
                                  TTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCC                                                                                                                 <  SRR19973081.645216/150‑1 (MQ=60)
                                  TTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCC                                                                                                                 <  SRR19973081.645228/150‑1 (MQ=60)
                                    AACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCG                                                                                                                >  SRR19973081.4420697/1‑150 (MQ=60)
                                    AACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCG                                                                                                                >  SRR19973081.3590584/1‑150 (MQ=60)
                                        CTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATG                                                                                                           <  SRR19973081.2060042/150‑1 (MQ=60)
                                        CTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATG                                                                                                           >  SRR19973081.2446617/1‑150 (MQ=60)
                                         TGCTCGTGCAATTGCCAGGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATG                                                                                                           <  SRR19973081.3371811/150‑1 (MQ=60)
                                            TCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATTATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGG                                                                                                       >  SRR19973081.1069171/1‑150 (MQ=60)
                                              GTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGT                                                                                                     <  SRR19973081.2675188/150‑1 (MQ=60)
                                                 CAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACG                                                                                                  >  SRR19973081.456820/1‑150 (MQ=60)
                                                   ATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCC                                                                                                <  SRR19973081.3986136/150‑1 (MQ=60)
                                                      GCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCG                                                                                              >  SRR19973081.4601310/1‑150 (MQ=60)
                                                      GCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCG                                                                                              >  SRR19973081.2917449/1‑150 (MQ=60)
                                                         ATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCA                                                                                           <  SRR19973081.3642819/150‑1 (MQ=60)
                                                         ATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAA                                                                                          >  SRR19973081.1871171/1‑150 (MQ=60)
                                                         ATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAA                                                                                          >  SRR19973081.2742486/1‑150 (MQ=60)
                                                          TGGGTGCAATTTTTATGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAA                                                                                          <  SRR19973081.259036/150‑1 (MQ=60)
                                                          TGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAAT                                                                                         >  SRR19973081.51707/1‑150 (MQ=60)
                                                              TGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAA                                                                                      >  SRR19973081.3816693/1‑150 (MQ=60)
                                                              TGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAG                                                                                     <  SRR19973081.1550992/150‑1 (MQ=60)
                                                              TGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAG                                                                                     <  SRR19973081.1549777/150‑1 (MQ=60)
                                                              TGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAG                                                                                     <  SRR19973081.1549723/150‑1 (MQ=60)
                                                               GCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAG                                                                                     >  SRR19973081.660577/1‑150 (MQ=60)
                                                                  ATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTT                                                                                 >  SRR19973081.172876/1‑150 (MQ=60)
                                                                  ATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTT                                                                                 >  SRR19973081.2618668/1‑150 (MQ=60)
                                                                       TAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAG                                                                            >  SRR19973081.448508/1‑150 (MQ=60)
                                                                          GGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGAT                                                                          >  SRR19973081.348944/1‑150 (MQ=60)
                                                                          GGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATA                                                                         >  SRR19973081.535000/1‑150 (MQ=60)
                                                                             GTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACC                                                                       >  SRR19973081.1672647/1‑150 (MQ=60)
                                                                               TGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCAC                                                                     >  SRR19973081.126934/1‑150 (MQ=60)
                                                                                 TTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGG                                                                  >  SRR19973081.3715212/1‑150 (MQ=60)
                                                                                 TTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGG                                                                  >  SRR19973081.4121397/1‑150 (MQ=60)
                                                                                  TATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGT                                                                 >  SRR19973081.1273563/1‑150 (MQ=60)
                                                                                      ATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCT                                                             <  SRR19973081.1276801/150‑1 (MQ=60)
                                                                                       TCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTG                                                            <  SRR19973081.310668/150‑1 (MQ=60)
                                                                                        CCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTAATCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGG                                                           >  SRR19973081.1855002/1‑150 (MQ=60)
                                                                                          ACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCA                                                         >  SRR19973081.1946773/1‑150 (MQ=60)
                                                                                              CTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGAC                                                     <  SRR19973081.2899925/150‑1 (MQ=60)
                                                                                              CTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGAC                                                     >  SRR19973081.382771/1‑150 (MQ=60)
                                                                                                    TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                               <  SRR19973081.2238693/150‑1 (MQ=60)
                                                                                                    TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                               <  SRR19973081.2238695/150‑1 (MQ=60)
                                                                                                    TAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTT                                               >  SRR19973081.1053724/1‑150 (MQ=60)
                                                                                                      AAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAA                                             >  SRR19973081.2587792/1‑150 (MQ=60)
                                                                                                      AAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACAACAGGTTTCTGGCATGACGATTTTAA                                             >  SRR19973081.2655856/1‑150 (MQ=60)
                                                                                                       AAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAA                                             >  SRR19973081.2273130/1‑150 (MQ=60)
                                                                                                       AAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAAT                                            >  SRR19973081.78286/1‑150 (MQ=60)
                                                                                                        AAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTATATACCACAGGTTTCTGGCATGACGATTTTAATA                                           >  SRR19973081.2152838/1‑150 (MQ=60)
                                                                                                        AAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATA                                           >  SRR19973081.1382697/1‑150 (MQ=60)
                                                                                                        AAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATA                                           >  SRR19973081.3858944/1‑150 (MQ=60)
                                                                                                        AAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATA                                           >  SRR19973081.2152807/1‑150 (MQ=60)
                                                                                                         AAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAA                                          <  SRR19973081.2773571/150‑1 (MQ=60)
                                                                                                           GGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACG                                        <  SRR19973081.2295947/150‑1 (MQ=60)
                                                                                                           GGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACG                                        <  SRR19973081.2298483/150‑1 (MQ=60)
                                                                                                            GGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACG                                        >  SRR19973081.3010881/1‑150 (MQ=60)
                                                                                                                     AGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGA                               >  SRR19973081.2759675/1‑150 (MQ=60)
                                                                                                                     AGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGA                               >  SRR19973081.2760981/1‑150 (MQ=60)
                                                                                                                           CGGTCAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATAT                        <  SRR19973081.932920/150‑1 (MQ=60)
                                                                                                                               CAGTTTAATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGC                    >  SRR19973081.207796/1‑150 (MQ=60)
                                                                                                                                AGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGC                    >  SRR19973081.1531897/1‑150 (MQ=60)
                                                                                                                                 GTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCG                   <  SRR19973081.935359/150‑1 (MQ=60)
                                                                                                                                 GTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCG                   <  SRR19973081.3391318/150‑1 (MQ=60)
                                                                                                                                 GTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCG                   >  SRR19973081.1840866/1‑150 (MQ=60)
                                                                                                                                      ATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATTTTTCCTTAATGATGGGTACGCCCGCCAATAAAGCGTTAATAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGAGTGGCAA             >  SRR19973081.817557/1‑150 (MQ=60)
                                                                                                                                      ATGATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAA             >  SRR19973081.862272/1‑150 (MQ=60)
                                                                                                                                        GATTATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATT           >  SRR19973081.426935/1‑150 (MQ=60)
                                                                                                                                            ATTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTA       >  SRR19973081.3159750/1‑150 (MQ=60)
                                                                                                                                             TTTCAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAG      >  SRR19973081.4365943/1‑150 (MQ=60)
                                                                                                                                               TCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGC     >  SRR19973081.490621/1‑150 (MQ=60)
                                                                                                                                                CAC‑AAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGA   >  SRR19973081.4364776/1‑150 (MQ=60)
                                                                                                                                                CAC‑AAAACTCAGCGAATCAGAAAAGGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTCGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTCGCGA   >  SRR19973081.4364767/1‑150 (MQ=60)
                                                                                                                                                  CGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAA  >  SRR19973081.2243040/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
TTTACTCGTTGTCATCGATACCGTAATCGCCACATTAACACTGCTCGTGCAATTGCCATGGGTGCAATTTTTAAGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAA  >  CP009273/542053‑542348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑