Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,252,258 C→A 92.5% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,252,2580CA92.5% 814.2 / 47.7 260D251Y (GAT→TAT) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base C (9/11);  new base A (126/114);  total (135/125)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCT  >  CP009273/3252111‑3252403
                                                                                                                                                   |                                                                                                                                                 
aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTCGAGATGTTATAGc                                                                                                                                                 >  2:4574059/1‑150 (MQ=255)
  taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTg                                                                                                                                               >  2:169883/1‑150 (MQ=255)
      ccAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGaaa                                                                                                                                           <  1:3205522/150‑1 (MQ=255)
       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                          >  2:459127/1‑150 (MQ=255)
       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                          >  1:221357/1‑150 (MQ=255)
        aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATAGGATCGTTAGAGATGTTATAGCTGGAAAcc                                                                                                                                         >  1:3541879/1‑150 (MQ=255)
         gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCa                                                                                                                                        <  1:590140/150‑1 (MQ=255)
          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAg                                                                                                                                       >  1:593583/1‑150 (MQ=255)
          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAACCCAg                                                                                                                                       >  1:5053186/1‑150 (MQ=255)
          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                       >  1:2810963/1‑150 (MQ=255)
           aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                      <  1:3129824/150‑1 (MQ=255)
           aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                      >  2:633715/1‑150 (MQ=255)
            cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCt                                                                                                                                     <  2:1334288/150‑1 (MQ=255)
                 ggAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacga                                                                                                                                >  2:3585536/1‑150 (MQ=255)
                   aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                              <  1:3489442/150‑1 (MQ=255)
                      aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAg                                                                                                                           <  2:2984080/150‑1 (MQ=255)
                       atGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgc                                                                                                                          >  1:3155934/1‑150 (MQ=255)
                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                      <  2:3022183/150‑1 (MQ=255)
                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                      >  2:510973/1‑150 (MQ=255)
                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                      <  1:415012/150‑1 (MQ=255)
                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                      <  2:3938792/150‑1 (MQ=255)
                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                      <  2:3531933/150‑1 (MQ=255)
                            gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTac                                                                                                                     >  2:4649690/1‑150 (MQ=255)
                            gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTac                                                                                                                     >  2:4650258/1‑150 (MQ=255)
                            gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTac                                                                                                                     <  2:2228368/150‑1 (MQ=255)
                               cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggc                                                                                                                  >  1:2155160/1‑150 (MQ=255)
                               cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCGTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggc                                                                                                                  <  2:4329642/150‑1 (MQ=255)
                                gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGATTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggca                                                                                                                 >  2:3016503/1‑150 (MQ=255)
                                 cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcac                                                                                                                <  2:2883768/150‑1 (MQ=255)
                                  acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcacg                                                                                                               >  1:802255/1‑150 (MQ=255)
                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcg                                                                                                            <  1:615973/150‑1 (MQ=255)
                                      ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcgc                                                                                                           <  1:1557432/150‑1 (MQ=255)
                                        aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCgg                                                                                                         >  2:3374160/1‑150 (MQ=255)
                                        aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCgg                                                                                                         >  2:1592330/1‑150 (MQ=255)
                                        aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCgg                                                                                                         <  2:5132284/150‑1 (MQ=255)
                                            ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGcaca                                                                                                     >  2:1956821/1‑150 (MQ=255)
                                            ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGcaca                                                                                                     >  2:4809232/1‑150 (MQ=255)
                                              tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAtt                                                                                                   >  1:3767828/1‑150 (MQ=255)
                                              tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAtt                                                                                                   <  2:2666136/150‑1 (MQ=255)
                                                  gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGc                                                                                               >  2:2818005/1‑150 (MQ=255)
                                                  gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACAGGTTGATCCAGTCGATGCCATTCATCGGATCGTTCGAGATGTTATAGCTGGAAACCAGCGGACGACGCAGCGCTACGGCACGGCGCGGCACATGGAGc                                                                                               >  2:3939426/1‑150 (MQ=255)
                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGa                                                                                            <  2:4332406/150‑1 (MQ=255)
                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                           <  1:752483/150‑1 (MQ=255)
                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                           <  1:2051073/150‑1 (MQ=255)
                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                           >  1:992615/1‑150 (MQ=255)
                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                           <  2:524738/150‑1 (MQ=255)
                                                         cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAg                                                                                        <  1:2546325/150‑1 (MQ=255)
                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                     >  1:1478401/1‑150 (MQ=255)
                                                             cccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgcag                                                                                    >  1:4400382/1‑150 (MQ=255)
                                                              ccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgcagc                                                                                   >  1:3337440/1‑150 (MQ=255)
                                                                   aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                              >  1:4675257/1‑150 (MQ=255)
                                                                     cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCtt                                                                            <  1:4613550/150‑1 (MQ=255)
                                                                     cTGCGTTTTCGTCACGAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCtt                                                                            <  1:4613585/150‑1 (MQ=255)
                                                                      tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTc                                                                           <  2:375217/150‑1 (MQ=255)
                                                                       gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCTTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCa                                                                          <  2:929419/150‑1 (MQ=255)
                                                                       gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCa                                                                          <  1:1810368/150‑1 (MQ=255)
                                                                         gTTTTCTTCACTAACCACCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgt                                                                        >  2:1084059/1‑150 (MQ=255)
                                                                            ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGtt                                                                     >  2:1979247/1‑150 (MQ=255)
                                                                            ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGtt                                                                     >  2:4611892/1‑150 (MQ=255)
                                                                              cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCa                                                                   <  1:4300778/150‑1 (MQ=255)
                                                                                   cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                              <  1:411642/150‑1 (MQ=255)
                                                                                   cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                              <  1:3055616/150‑1 (MQ=255)
                                                                                      aCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGt                                                           >  2:1237799/1‑150 (MQ=255)
                                                                                       ccGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGtt                                                          >  2:4273599/1‑150 (MQ=255)
                                                                                          ccAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCGTCAGTGTTCATCCCACGTTCaa                                                       >  1:1321435/1‑150 (MQ=255)
                                                                                           cAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                      >  1:2881727/1‑150 (MQ=255)
                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                   <  1:2092813/150‑1 (MQ=255)
                                                                                                  tACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCa                                               <  2:1272134/150‑1 (MQ=255)
                                                                                                   aCATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAt                                              <  1:954392/150‑1 (MQ=255)
                                                                                                   aCATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAt                                              >  2:305174/1‑150 (MQ=255)
                                                                                                    cATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                             <  1:4914261/150‑1 (MQ=255)
                                                                                                          gATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTTGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAAACCGTCATGCATCAc                                       >  1:2237824/1‑150 (MQ=255)
                                                                                                          gATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAc                                       >  1:2450553/1‑150 (MQ=255)
                                                                                                          gATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAc                                       >  2:685089/1‑150 (MQ=255)
                                                                                                            tCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACtt                                     <  1:5010166/150‑1 (MQ=255)
                                                                                                             ccAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                    <  1:1592380/150‑1 (MQ=255)
                                                                                                               aGTCGATGACATTCATCGGATCGTTAGATATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                  <  1:1014416/150‑1 (MQ=255)
                                                                                                               aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                  >  2:889681/1‑150 (MQ=255)
                                                                                                               aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                  <  1:169883/150‑1 (MQ=255)
                                                                                                                  cGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                               <  2:2347409/150‑1 (MQ=255)
                                                                                                                    aTGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATa                             <  1:3942967/150‑1 (MQ=255)
                                                                                                                         aTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                        >  2:991400/1‑150 (MQ=255)
                                                                                                                         aTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                        >  2:3112995/1‑150 (MQ=255)
                                                                                                                         aTTCATCGGATCGTTAGAGATGTTAGAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                        <  1:2703884/150‑1 (MQ=255)
                                                                                                                           tCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaa                      <  2:2521098/150‑1 (MQ=255)
                                                                                                                            cATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaa                     <  1:843235/150‑1 (MQ=255)
                                                                                                                             aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                    >  2:809449/1‑150 (MQ=255)
                                                                                                                             aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                    >  2:4134943/1‑150 (MQ=255)
                                                                                                                             aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                    >  2:1220962/1‑150 (MQ=255)
                                                                                                                                ggATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCg                 >  2:4078863/1‑150 (MQ=255)
                                                                                                                                 gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg                >  1:3342016/1‑150 (MQ=255)
                                                                                                                                    cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt             >  2:1454372/1‑150 (MQ=255)
                                                                                                                                         gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt        <  1:2573232/150‑1 (MQ=255)
                                                                                                                                         gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt        >  1:2293048/1‑150 (MQ=255)
                                                                                                                                          agaTGTTAGAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAttt       <  2:210089/150‑1 (MQ=255)
                                                                                                                                           gaTGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTc      <  1:3171461/150‑1 (MQ=255)
                                                                                                                                           gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTc      >  2:4891856/1‑150 (MQ=255)
                                                                                                                                            aTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTcc     >  2:835811/1‑150 (MQ=255)
                                                                                                                                              gTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCACTTTCCGc   >  2:584986/1‑150 (MQ=255)
                                                                                                                                               ttATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCt  >  1:2433032/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCT  >  CP009273/3252111‑3252403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  >  CP009273/3252109‑3252406
                                                                                                                                                     |                                                                                                                                                    
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTCCATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATA                                                                                                                                                      >  SRR19973083.1707310/1‑150 (MQ=60)
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATA                                                                                                                                                      >  SRR19973083.1808090/1‑150 (MQ=60)
  AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGC                                                                                                                                                    >  SRR19973083.376967/1‑150 (MQ=60)
  AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGC                                                                                                                                                    >  SRR19973083.5069823/1‑150 (MQ=60)
   ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCT                                                                                                                                                   <  SRR19973083.1685206/150‑1 (MQ=60)
       GCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAA                                                                                                                                               <  SRR19973083.2584236/150‑1 (MQ=60)
       GCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAA                                                                                                                                               <  SRR19973083.245611/150‑1 (MQ=60)
        CCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAA                                                                                                                                              <  SRR19973083.3220742/150‑1 (MQ=60)
         CAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAC                                                                                                                                             >  SRR19973083.222566/1‑150 (MQ=60)
         CAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAC                                                                                                                                             >  SRR19973083.222740/1‑150 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACC                                                                                                                                            >  SRR19973083.3558121/1‑150 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACC                                                                                                                                            <  SRR19973083.927754/150‑1 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATAGGATCGTTAGAGATGTTATAGCTGGAAACC                                                                                                                                            >  SRR19973083.3558141/1‑150 (MQ=60)
           GTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCA                                                                                                                                           <  SRR19973083.593364/150‑1 (MQ=60)
           GTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCA                                                                                                                                           <  SRR19973083.593109/150‑1 (MQ=60)
            TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAG                                                                                                                                          >  SRR19973083.596567/1‑150 (MQ=60)
            TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAACCCAG                                                                                                                                          >  SRR19973083.5074827/1‑150 (MQ=60)
            TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAG                                                                                                                                          >  SRR19973083.2824896/1‑150 (MQ=60)
             ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                         <  SRR19973083.3144585/150‑1 (MQ=60)
             ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                         <  SRR19973083.3144789/150‑1 (MQ=60)
             ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                         <  SRR19973083.3144834/150‑1 (MQ=60)
              CTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCT                                                                                                                                        >  SRR19973083.1688681/1‑150 (MQ=60)
                    GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                  <  SRR19973083.705294/150‑1 (MQ=60)
                    GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                  <  SRR19973083.30001/150‑1 (MQ=60)
                     AATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACG                                                                                                                                 <  SRR19973083.3505410/150‑1 (MQ=60)
                      ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGC                                                                                                                                >  SRR19973083.225920/1‑150 (MQ=60)
                      ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGC                                                                                                                                >  SRR19973083.226753/1‑150 (MQ=60)
                         ATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGC                                                                                                                             >  SRR19973083.3170975/1‑150 (MQ=60)
                         ATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGC                                                                                                                             >  SRR19973083.3170995/1‑150 (MQ=60)
                             CGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTA                                                                                                                         <  SRR19973083.416875/150‑1 (MQ=60)
                              GCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTAC                                                                                                                        <  SRR19973083.3843332/150‑1 (MQ=60)
                                 CGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGC                                                                                                                     >  SRR19973083.2166398/1‑150 (MQ=60)
                                 CGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGC                                                                                                                     >  SRR19973083.2165975/1‑150 (MQ=60)
                                    ACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACG                                                                                                                  >  SRR19973083.806009/1‑150 (MQ=60)
                                    ACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACG                                                                                                                  >  SRR19973083.806106/1‑150 (MQ=60)
                                      CGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGC                                                                                                                >  SRR19973083.3324484/1‑150 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                               <  SRR19973083.618945/150‑1 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                               <  SRR19973083.619063/150‑1 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                               >  SRR19973083.2479869/1‑150 (MQ=60)
                                        TTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGC                                                                                                              <  SRR19973083.1565671/150‑1 (MQ=60)
                                        TTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGC                                                                                                              <  SRR19973083.1565259/150‑1 (MQ=60)
                                              GGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACA                                                                                                        >  SRR19973083.816069/1‑150 (MQ=60)
                                                TGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATT                                                                                                      >  SRR19973083.4971285/1‑150 (MQ=60)
                                                TGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATT                                                                                                      >  SRR19973083.3784870/1‑150 (MQ=60)
                                                   CGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAG                                                                                                   >  SRR19973083.4586993/1‑150 (MQ=60)
                                                    GTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGC                                                                                                  <  SRR19973083.494958/150‑1 (MQ=60)
                                                     TTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCG                                                                                                 <  SRR19973083.690318/150‑1 (MQ=60)
                                                      TACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGG                                                                                                <  SRR19973083.2586545/150‑1 (MQ=60)
                                                        CCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAC                                                                                              <  SRR19973083.756169/150‑1 (MQ=60)
                                                        CCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAC                                                                                              <  SRR19973083.759451/150‑1 (MQ=60)
                                                        CCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAC                                                                                              <  SRR19973083.2061529/150‑1 (MQ=60)
                                                        CCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAC                                                                                              >  SRR19973083.997574/1‑150 (MQ=60)
                                                          ACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCA                                                                                            >  SRR19973083.5207197/1‑150 (MQ=60)
                                                          ACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCA                                                                                            <  SRR19973083.188529/150‑1 (MQ=60)
                                                           CGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAG                                                                                           <  SRR19973083.2559216/150‑1 (MQ=60)
                                                              GCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCA                                                                                        >  SRR19973083.1485851/1‑150 (MQ=60)
                                                               CCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACCTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAGTGAGCGGACCAGGCAG                                                                                       >  SRR19973083.596177/1‑150 (MQ=60)
                                                               CCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAG                                                                                       >  SRR19973083.4419527/1‑150 (MQ=60)
                                                               CCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAG                                                                                       >  SRR19973083.2798040/1‑150 (MQ=60)
                                                               CCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAG                                                                                       >  SRR19973083.4419261/1‑150 (MQ=60)
                                                                CCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGC                                                                                      >  SRR19973083.3353032/1‑150 (MQ=60)
                                                                  GCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCAC                                                                                    >  SRR19973083.2159084/1‑150 (MQ=60)
                                                                     AGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCC                                                                                 >  SRR19973083.4695348/1‑150 (MQ=60)
                                                                     AGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCC                                                                                 >  SRR19973083.4695351/1‑150 (MQ=60)
                                                                       CTGCGTTTTCTTCACTACCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                               <  SRR19973083.4633492/150‑1 (MQ=60)
                                                                       CTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                               <  SRR19973083.4633465/150‑1 (MQ=60)
                                                                       CTGCGTTTTCGTCACGAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                               <  SRR19973083.4633500/150‑1 (MQ=60)
                                                                        TGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTC                                                                              <  SRR19973083.4380416/150‑1 (MQ=60)
                                                                         GCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCA                                                                             <  SRR19973083.4589409/150‑1 (MQ=60)
                                                                         GCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCA                                                                             <  SRR19973083.1819459/150‑1 (MQ=60)
                                                                                CTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCA                                                                      <  SRR19973083.4319494/150‑1 (MQ=60)
                                                                                CTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCA                                                                      <  SRR19973083.1715449/150‑1 (MQ=60)
                                                                                CTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCA                                                                      <  SRR19973083.1407192/150‑1 (MQ=60)
                                                                                CTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCA                                                                      <  SRR19973083.2350889/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                 <  SRR19973083.413495/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                 <  SRR19973083.3191124/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                 <  SRR19973083.3344238/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                 <  SRR19973083.3070258/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                 <  SRR19973083.3191089/150‑1 (MQ=60)
                                                                                      TAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCAC                                                                <  SRR19973083.2122062/150‑1 (MQ=60)
                                                                                          CGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTC                                                            >  SRR19973083.2892187/1‑150 (MQ=60)
                                                                                           GCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCA                                                           <  SRR19973083.4006914/150‑1 (MQ=60)
                                                                                            CCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCGTCAGTGTTCATCCCACGTTCAA                                                          >  SRR19973083.1327818/1‑150 (MQ=60)
                                                                                             CAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAT                                                         >  SRR19973083.2895884/1‑150 (MQ=60)
                                                                                                CGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACC                                                      <  SRR19973083.2103423/150‑1 (MQ=60)
                                                                                                 GCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCG                                                     >  SRR19973083.118164/1‑150 (MQ=60)
                                                                                                 GCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCG                                                     >  SRR19973083.2884469/1‑150 (MQ=60)
                                                                                                   GTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTC                                                   <  SRR19973083.3180657/150‑1 (MQ=60)
                                                                                                    TACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCA                                                  <  SRR19973083.976837/150‑1 (MQ=60)
                                                                                                    TACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCA                                                  <  SRR19973083.3475061/150‑1 (MQ=60)
                                                                                                     ACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAT                                                 <  SRR19973083.4643218/150‑1 (MQ=60)
                                                                                                     ACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAT                                                 <  SRR19973083.959101/150‑1 (MQ=60)
                                                                                                      CATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATG                                                <  SRR19973083.4935382/150‑1 (MQ=60)
                                                                                                         GTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATTCAT                                             >  SRR19973083.4143063/1‑150 (MQ=60)
                                                                                                         GTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCAT                                             <  SRR19973083.219637/150‑1 (MQ=60)
                                                                                                           TGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCA                                           <  SRR19973083.2994080/150‑1 (MQ=60)
                                                                                                            GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTTGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAAACCGTCATGCATCAC                                          >  SRR19973083.2249185/1‑150 (MQ=60)
                                                                                                            GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                          >  SRR19973083.2555125/1‑150 (MQ=60)
                                                                                                            GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                          >  SRR19973083.2463108/1‑150 (MQ=60)
                                                                                                            GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                          >  SRR19973083.5051477/1‑150 (MQ=60)
                                                                                                            GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                          <  SRR19973083.2076603/150‑1 (MQ=60)
                                                                                                              TCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTT                                        <  SRR19973083.5031658/150‑1 (MQ=60)
                                                                                                               CCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTG                                       <  SRR19973083.1600306/150‑1 (MQ=60)
                                                                                                               CCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTG                                       <  SRR19973083.1600357/150‑1 (MQ=60)
                                                                                                                CAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGC                                      >  SRR19973083.4164103/1‑150 (MQ=60)
                                                                                                                 AGTCGATGACATTCATCGGATCGTTAGATATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCC                                     <  SRR19973083.1019493/150‑1 (MQ=60)
                                                                                                                 AGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCC                                     <  SRR19973083.170864/150‑1 (MQ=60)
                                                                                                                  GTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCA                                    <  SRR19973083.1454163/150‑1 (MQ=60)
                                                                                                                    CGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGA                                  >  SRR19973083.1515728/1‑150 (MQ=60)
                                                                                                                    CGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGA                                  <  SRR19973083.4182063/150‑1 (MQ=60)
                                                                                                                    CGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGA                                  >  SRR19973083.1918981/1‑150 (MQ=60)
                                                                                                                      ATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATA                                <  SRR19973083.3960519/150‑1 (MQ=60)
                                                                                                                      ATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATA                                <  SRR19973083.3408888/150‑1 (MQ=60)
                                                                                                                           ATTCATCGGATCGTTAGAGATGTTAGAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                           <  SRR19973083.2717418/150‑1 (MQ=60)
                                                                                                                              CATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAA                        <  SRR19973083.847221/150‑1 (MQ=60)
                                                                                                                                TCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAACC                      <  SRR19973083.3704365/150‑1 (MQ=60)
                                                                                                                                TCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAAC                      <  SRR19973083.3960047/150‑1 (MQ=60)
                                                                                                                                 CGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACC                     <  SRR19973083.4733193/150‑1 (MQ=60)
                                                                                                                                   GATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGG                   >  SRR19973083.3357607/1‑150 (MQ=60)
                                                                                                                                   GATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGG                   >  SRR19973083.3357615/1‑150 (MQ=60)
                                                                                                                                           GAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATT           >  SRR19973083.2304795/1‑150 (MQ=60)
                                                                                                                                           GAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATT           <  SRR19973083.2586210/150‑1 (MQ=60)
                                                                                                                                           GAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATT           <  SRR19973083.2586238/150‑1 (MQ=60)
                                                                                                                                             GATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTC         <  SRR19973083.3186578/150‑1 (MQ=60)
                                                                                                                                               TGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCG       >  SRR19973083.860188/1‑150 (MQ=60)
                                                                                                                                                 TTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCT     >  SRR19973083.2445509/1‑150 (MQ=60)
                                                                                                                                                 TTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTACATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCT     >  SRR19973083.2445579/1‑150 (MQ=60)
                                                                                                                                                   ATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT   <  SRR19973083.1103333/150‑1 (MQ=60)
                                                                                                                                                    TAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  >  SRR19973083.1601762/1‑150 (MQ=60)
                                                                                                                                                    TAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  >  SRR19973083.2768225/1‑150 (MQ=60)
                                                                                                                                                    TAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  <  SRR19973083.4593845/150‑1 (MQ=60)
                                                                                                                                                    TAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  <  SRR19973083.3220192/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTG  >  CP009273/3252109‑3252406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑