Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,252,258	C→A	100%	D251Y (GAT→TAT)	tdcG ←	L‑serine dehydratase 3, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,252,258	0	C	A	99.6%	848.0 / ‑3.9	237	D251Y (GAT→TAT)	tdcG	L‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (132/104); total (133/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT  >  CP009273/3252111‑3252404

aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATATGATAGTTAGAGATGTTATAGc                                                                                                                                                  >  1:3676648/1‑150 (MQ=255)

aataaGACAGTACTGCCGGAATAATGCCGCACGCACCGTTAGACGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTATCTTCACAAACCGCCAGCGCGAACATGTTGATCCAGTCGATGACATACATCGGATCGTTAGAGAGGTTATAGc                                                                                                                                                  >  2:77444/1‑150 (MQ=255)

  taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTg                                                                                                                                                >  1:2501410/1‑150 (MQ=255)

   aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTgg                                                                                                                                               <  2:1980311/150‑1 (MQ=255)

       cAGTACTGCCGGAATAATGCCGCACGCTCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           <  1:3815937/150‑1 (MQ=255)

       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           >  2:2817071/1‑150 (MQ=255)

       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                           <  1:1902469/150‑1 (MQ=255)

        aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAcc                                                                                                                                          <  2:2129896/150‑1 (MQ=255)

          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                        <  1:636140/150‑1 (MQ=255)

          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                        <  1:403838/150‑1 (MQ=255)

           aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                       >  1:1003071/1‑150 (MQ=255)

             tGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTg                                                                                                                                     <  1:2459061/150‑1 (MQ=255)

              gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTga                                                                                                                                    >  1:2656209/1‑150 (MQ=255)

                cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacg                                                                                                                                  <  1:2042083/150‑1 (MQ=255)

                  gAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacgac                                                                                                                                <  1:3110484/150‑1 (MQ=255)

                   aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                               >  2:2976467/1‑150 (MQ=255)

                   aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                               >  1:235567/1‑150 (MQ=255)

                        tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcg                                                                                                                          >  1:3349289/1‑150 (MQ=255)

                        tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcg                                                                                                                          >  1:3110572/1‑150 (MQ=255)

                          ccGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCt                                                                                                                        <  2:3149181/150‑1 (MQ=255)

                          ccGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCt                                                                                                                        >  2:3025872/1‑150 (MQ=255)

                             cacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACAGGTAGATCCAGACGATGACATTCAACGGATCGTTAGAGAAGTTATAGCTGGAAACCAGCAGACGACGCAGCGCTacg                                                                                                                     >  1:1874767/1‑150 (MQ=255)

                                gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggca                                                                                                                  >  1:3889949/1‑150 (MQ=255)

                                gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggca                                                                                                                  >  1:2501685/1‑150 (MQ=255)

                                 cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcac                                                                                                                 >  1:1081134/1‑150 (MQ=255)

                                 cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcac                                                                                                                 >  1:3392442/1‑150 (MQ=255)

                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcg                                                                                                             >  1:4083736/1‑150 (MQ=255)

                                      ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcgc                                                                                                            >  1:2989069/1‑150 (MQ=255)

                                       tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGcgcg                                                                                                           <  2:1014266/150‑1 (MQ=255)

                                        aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCgg                                                                                                          >  2:392161/1‑150 (MQ=255)

                                             gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAt                                                                                                     <  2:2064082/150‑1 (MQ=255)

                                                 cgatACCACGCGCCCGCCAGCTGCGTTTTATTCACTAACCGCCAGCGCGTACATGTTGAACCAGTCGATGACATTCATCGGATCGTTAGATAAGTTATAGCTGGAAACCAGCTGACGACGCATCCCTACGGCACGGCGCGGCACATTGAg                                                                                                 >  1:3110702/4‑150 (MQ=37)

                                                   ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCg                                                                                               <  1:2876106/150‑1 (MQ=255)

                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAc                                                                                            <  1:2746357/150‑1 (MQ=255)

                                                        aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCa                                                                                          <  1:1617769/150‑1 (MQ=255)

                                                         cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAg                                                                                         <  2:3409619/150‑1 (MQ=255)

                                                          gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAgg                                                                                        <  2:2577734/150‑1 (MQ=255)

                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:2011906/150‑1 (MQ=255)

                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:368962/150‑1 (MQ=255)

                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  1:619519/150‑1 (MQ=255)

                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgca                                                                                      <  2:3210056/150‑1 (MQ=255)

                                                                   aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCCGGCAGCACGcc                                                                               >  1:700367/1‑150 (MQ=255)

                                                                    gCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCt                                                                              >  2:1346775/1‑150 (MQ=255)

                                                                         gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgt                                                                         >  2:2680846/1‑150 (MQ=255)

                                                                            ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGtt                                                                      <  1:1525268/150‑1 (MQ=255)

                                                                             tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                     >  1:3864015/1‑150 (MQ=255)

                                                                             tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                     >  1:40454/1‑150 (MQ=255)

                                                                               ttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAt                                                                   <  1:2527866/150‑1 (MQ=255)

                                                                                tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATc                                                                  >  2:1806299/1‑150 (MQ=255)

                                                                                 cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                                 >  2:2333688/1‑150 (MQ=255)

                                                                                 cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                                 <  2:897569/150‑1 (MQ=255)

                                                                                  aCTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                                >  2:385323/1‑150 (MQ=255)

                                                                                  aCTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                                >  2:1614796/1‑150 (MQ=255)

                                                                                   cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                               <  1:1434062/150‑1 (MQ=255)

                                                                                        cGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                          >  1:1548941/1‑150 (MQ=255)

                                                                                        cGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                          >  2:3535729/1‑150 (MQ=255)

                                                                                           cAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                       <  2:2813321/150‑1 (MQ=255)

                                                                                           cAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                       >  2:4040693/1‑150 (MQ=255)

                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCCGTGTTCATCCCACGTTCAATAcc                                                    <  2:2902781/150‑1 (MQ=255)

                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:2053569/150‑1 (MQ=255)

                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:1259167/150‑1 (MQ=255)

                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATAcc                                                    <  2:1042663/150‑1 (MQ=255)

                                                                                              cgcgTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGAAGACGCAGAGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGATCATCCCACGTTCAATAcc                                                    >  2:1553517/1‑150 (MQ=255)

                                                                                                 gTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                                 >  2:1412915/1‑150 (MQ=255)

                                                                                                 gTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                                 >  2:3703230/1‑150 (MQ=255)

                                                                                                  tACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCa                                                <  1:779629/150‑1 (MQ=255)

                                                                                                    cATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                              >  1:1797169/1‑150 (MQ=255)

                                                                                                    cATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                              >  1:938451/1‑150 (MQ=255)

                                                                                                     aTGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGc                                             >  1:328623/1‑150 (MQ=255)

                                                                                                        ttGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATc                                          <  2:1712313/150‑1 (MQ=255)

                                                                                                        ttGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATc                                          <  2:594204/150‑1 (MQ=255)

                                                                                                          gATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAc                                        >  1:2270515/1‑150 (MQ=255)

                                                                                                           aTCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACt                                       >  2:3228543/1‑150 (MQ=255)

                                                                                                             ccAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                     >  1:4169461/1‑150 (MQ=255)

                                                                                                               aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                   >  1:1213122/1‑150 (MQ=255)

                                                                                                                   gATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                               >  2:376252/1‑150 (MQ=255)

                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            <  1:455744/150‑1 (MQ=255)

                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            >  1:2135711/1‑150 (MQ=255)

                                                                                                                      gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                            >  1:2935202/1‑150 (MQ=255)

                                                                                                                        cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACggg                          >  2:3666129/1‑150 (MQ=255)

                                                                                                                         aTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                         >  1:830816/1‑150 (MQ=255)

                                                                                                                                ggATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCg                  <  1:2510960/150‑1 (MQ=255)

                                                                                                                                 gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg                 <  2:230851/150‑1 (MQ=255)

                                                                                                                                 gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg                 >  2:1116655/1‑150 (MQ=255)

                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  2:874759/1‑150 (MQ=255)

                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  2:362446/1‑150 (MQ=255)

                                                                                                                                  aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc                >  1:2615430/1‑150 (MQ=255)

                                                                                                                                    cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt              <  1:1683952/150‑1 (MQ=255)

                                                                                                                                    cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt              >  1:914689/1‑150 (MQ=255)

                                                                                                                                     gTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc             <  1:3784948/150‑1 (MQ=255)

                                                                                                                                     gTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc             >  1:1168115/1‑150 (MQ=255)

                                                                                                                                       tAGAGATGTTATAGCTGGAAACCATCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  1:1041070/1‑150 (MQ=255)

                                                                                                                                       tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  2:3336528/1‑150 (MQ=255)

                                                                                                                                       tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa           >  1:3476177/1‑150 (MQ=255)

                                                                                                                                        agagATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAt          >  1:2468327/1‑150 (MQ=255)

                                                                                                                                        agagATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAt          <  2:2424324/150‑1 (MQ=255)

                                                                                                                                         gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt         >  2:1867654/1‑150 (MQ=255)

                                                                                                                                          agaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtct        <  1:2641265/150‑3 (MQ=255)

                                                                                                                                           gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTc       <  1:2805243/150‑1 (MQ=255)

                                                                                                                                                tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCtt  >  1:3837979/1‑150 (MQ=255)

AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT  >  CP009273/3252111‑3252404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGC  >  CP009273/3252110‑3252407

TAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAG                                                                                                                                                      <  SRR19973074.132156/150‑1 (MQ=60)

 AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGC                                                                                                                                                     <  SRR19973074.3191805/150‑1 (MQ=60)

 AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATATGATAGTTAGAGATGTTATAGC                                                                                                                                                     >  SRR19973074.3683444/1‑150 (MQ=60)

  ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCT                                                                                                                                                    <  SRR19973074.73301/150‑1 (MQ=60)

   TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                   >  SRR19973074.2506063/1‑150 (MQ=60)

   TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                   >  SRR19973074.2505971/1‑150 (MQ=60)

   TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                   >  SRR19973074.2817232/1‑150 (MQ=60)

        CAGTACTGCCGGAATAATGCCGCACGCTCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAC                                                                                                                                              <  SRR19973074.3822903/150‑1 (MQ=60)

        CAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAC                                                                                                                                              <  SRR19973074.1906115/150‑1 (MQ=60)

         AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACC                                                                                                                                             <  SRR19973074.2633862/150‑1 (MQ=60)

         AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACC                                                                                                                                             <  SRR19973074.2633867/150‑1 (MQ=60)

          GTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCA                                                                                                                                            <  SRR19973074.1101758/150‑1 (MQ=60)

           TACTGCCGGAATAATGCCGCTCGCACGGTTAGTAGGTGCCGTTTCCTCGCGCCCGGCAGGTGCGTTTTCTTAACTAACCGCCTGCGCGTACTTGTTGATCCTGTCGATGACATTATTCGGTTCGTTAGATATGTTATTTCTGGTAACCAG                                                                                                                                           <  SRR19973074.3790836/150‑1 (MQ=56)

           TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAG                                                                                                                                           <  SRR19973074.637329/150‑1 (MQ=60)

           TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAG                                                                                                                                           <  SRR19973074.404633/150‑1 (MQ=60)

           TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAG                                                                                                                                           <  SRR19973074.404612/150‑1 (MQ=60)

            ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                          >  SRR19973074.1004933/1‑150 (MQ=60)

            ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                          >  SRR19973074.3843365/1‑150 (MQ=60)

            ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                          >  SRR19973074.926599/1‑150 (MQ=60)

              TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTG                                                                                                                                        <  SRR19973074.4088425/150‑1 (MQ=60)

              TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTG                                                                                                                                        <  SRR19973074.2463617/150‑1 (MQ=60)

              TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTG                                                                                                                                        <  SRR19973074.2463610/150‑1 (MQ=60)

              TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTG                                                                                                                                        <  SRR19973074.3424996/150‑1 (MQ=60)

              TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTG                                                                                                                                        <  SRR19973074.2756145/150‑1 (MQ=60)

               GCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGA                                                                                                                                       >  SRR19973074.2661159/1‑150 (MQ=60)

                 CGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACG                                                                                                                                     <  SRR19973074.2045840/150‑1 (MQ=60)

                 CGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACG                                                                                                                                     <  SRR19973074.2045930/150‑1 (MQ=60)

                   GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                   >  SRR19973074.868350/1‑150 (MQ=60)

                   GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                   <  SRR19973074.3116191/150‑1 (MQ=60)

                   GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                   >  SRR19973074.868203/1‑150 (MQ=60)

                    AATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACG                                                                                                                                  >  SRR19973074.236049/1‑150 (MQ=60)

                       AANGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAG                                                                                                                               >  SRR19973074.65134/1‑150 (MQ=60)

                         TGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCG                                                                                                                             <  SRR19973074.1538365/150‑1 (MQ=60)

                         TGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCG                                                                                                                             >  SRR19973074.3116270/1‑150 (MQ=60)

                         TGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCG                                                                                                                             >  SRR19973074.3355506/1‑150 (MQ=60)

                         TGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCG                                                                                                                             >  SRR19973074.3116279/1‑150 (MQ=60)

                          GCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGC                                                                                                                            >  SRR19973074.3812394/1‑150 (MQ=60)

                              CACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACG                                                                                                                        >  SRR19973074.1878208/1‑150 (MQ=60)

                              CACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACG                                                                                                                        <  SRR19973074.1230781/150‑1 (MQ=60)

                              CACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACAGGTAGATCCAGACGATGACATTCAACGGATCGTTAGAGAAGTTATAGCTGGAAACCAGCAGACGACGCAGCGCTACG                                                                                                                        >  SRR19973074.1878355/1‑150 (MQ=60)

                                 GCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCA                                                                                                                     >  SRR19973074.3897074/1‑150 (MQ=60)

                                 GCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCA                                                                                                                     >  SRR19973074.2506338/1‑150 (MQ=60)

                                 GCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCA                                                                                                                     >  SRR19973074.2506334/1‑150 (MQ=60)

                                  CACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCAC                                                                                                                    >  SRR19973074.3398723/1‑150 (MQ=60)

                                  CACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCAC                                                                                                                    >  SRR19973074.1083153/1‑150 (MQ=60)

                                  CACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCAC                                                                                                                    >  SRR19973074.1083070/1‑150 (MQ=60)

                                      GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                                >  SRR19973074.4091292/1‑150 (MQ=60)

                                      GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                                >  SRR19973074.267002/1‑150 (MQ=60)

                                       TTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGC                                                                                                               >  SRR19973074.2994626/1‑150 (MQ=60)

                                       TTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGC                                                                                                               >  SRR19973074.2994811/1‑150 (MQ=60)

                                            CGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCAC                                                                                                          >  SRR19973074.1014743/1‑150 (MQ=60)

                                                  CGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAG                                                                                                    >  SRR19973074.3116397/1‑150 (MQ=60)

                                                  CGATACCACGCGCCCGCCAGCTGCGTTTTATTCACTAACCGCCAGCGCGTACATGTTGAACCAGTCGATGACATTCATCGGATCGTTAGATAAGTTATAGCTGGAAACCAGCTGACGACGCATCCCTACGGCACGGCGCGGCACATTGAG                                                                                                    >  SRR19973074.3116409/1‑150 (MQ=60)

                                                   GTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGC                                                                                                   >  SRR19973074.2208296/1‑150 (MQ=60)

                                                   GTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGC                                                                                                   >  SRR19973074.1899092/1‑150 (MQ=60)

                                                   GTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGC                                                                                                   <  SRR19973074.3347318/150‑1 (MQ=60)

                                                    TTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCG                                                                                                  <  SRR19973074.3702222/150‑1 (MQ=60)

                                                    TTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCG                                                                                                  <  SRR19973074.2881444/150‑1 (MQ=60)

                                                       CCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGAC                                                                                               <  SRR19973074.2751418/150‑1 (MQ=60)

                                                         ACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCA                                                                                             <  SRR19973074.1580680/150‑1 (MQ=60)

                                                         ACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCA                                                                                             <  SRR19973074.1620841/150‑1 (MQ=60)

                                                             GCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCA                                                                                         <  SRR19973074.620690/150‑1 (MQ=60)

                                                             GCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCA                                                                                         <  SRR19973074.561862/150‑1 (MQ=60)

                                                                    AGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCCGGCAGCACGCC                                                                                  >  SRR19973074.701662/1‑150 (MQ=60)

                                                                      CTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                                <  SRR19973074.622085/150‑1 (MQ=60)

                                                                        GCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCA                                                                              <  SRR19973074.2933996/150‑1 (MQ=60)

                                                                          GTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGT                                                                            <  SRR19973074.2305500/150‑1 (MQ=60)

                                                                             TTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTT                                                                         <  SRR19973074.1528087/150‑1 (MQ=60)

                                                                             TTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTT                                                                         <  SRR19973074.1528149/150‑1 (MQ=60)

                                                                              TCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTC                                                                        >  SRR19973074.3871071/1‑150 (MQ=60)

                                                                              TCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTC                                                                        >  SRR19973074.3871106/1‑150 (MQ=60)

                                                                              TCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTC                                                                        <  SRR19973074.3912045/150‑1 (MQ=60)

                                                                              TCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTC                                                                        >  SRR19973074.40573/1‑150 (MQ=60)

                                                                                TTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAT                                                                      <  SRR19973074.3899512/150‑1 (MQ=60)

                                                                                TTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAT                                                                      <  SRR19973074.2532578/150‑1 (MQ=60)

                                                                                    CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                  <  SRR19973074.1436724/150‑1 (MQ=60)

                                                                                     TAACCGCCAGCGCGTACATGGTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCAC                                                                 <  SRR19973074.2449702/150‑1 (MQ=60)

                                                                                         CGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTC                                                             >  SRR19973074.1551884/1‑150 (MQ=60)

                                                                                             AGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATA                                                         <  SRR19973074.2737440/150‑1 (MQ=60)

                                                                                             AGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATA                                                         <  SRR19973074.4173443/150‑1 (MQ=60)

                                                                                                   TACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCA                                                   >  SRR19973074.756644/1‑150 (MQ=60)

                                                                                                   TACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCA                                                   >  SRR19973074.2318658/1‑150 (MQ=60)

                                                                                                   TACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCA                                                   <  SRR19973074.781129/150‑1 (MQ=60)

                                                                                                     CATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATG                                                 >  SRR19973074.940241/1‑150 (MQ=60)

                                                                                                     CATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATG                                                 >  SRR19973074.1800584/1‑150 (MQ=60)

                                                                                                      ATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGC                                                >  SRR19973074.329219/1‑150 (MQ=60)

                                                                                                      ATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGC                                                >  SRR19973074.3366316/1‑150 (MQ=60)

                                                                                                      ATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGC                                                >  SRR19973074.3366401/1‑150 (MQ=60)

                                                                                                        GTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCAT                                              <  SRR19973074.171353/150‑1 (MQ=60)

                                                                                                          TGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCA                                            <  SRR19973074.1686793/150‑1 (MQ=60)

                                                                                                           GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                           >  SRR19973074.2274780/1‑150 (MQ=60)

                                                                                                           GATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAC                                           >  SRR19973074.3868975/1‑150 (MQ=60)

                                                                                                              CCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTG                                        >  SRR19973074.4178385/1‑150 (MQ=60)

                                                                                                              CCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTG                                        >  SRR19973074.4177124/1‑150 (MQ=60)

                                                                                                                AGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCC                                      >  SRR19973074.252177/1‑150 (MQ=60)

                                                                                                                AGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCC                                      >  SRR19973074.1215450/1‑150 (MQ=60)

                                                                                                                AGTCGATGACATTCATCGGATCGTTAGAGATGTAATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCC                                      >  SRR19973074.2569153/1‑150 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               <  SRR19973074.456634/150‑1 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               >  SRR19973074.2940661/1‑150 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               >  SRR19973074.2941125/1‑150 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               >  SRR19973074.2139720/1‑150 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               >  SRR19973074.83190/1‑150 (MQ=60)

                                                                                                                       GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                               >  SRR19973074.2139723/1‑150 (MQ=60)

                                                                                                                          ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                            >  SRR19973074.591533/1‑150 (MQ=60)

                                                                                                                          ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                            >  SRR19973074.1456612/1‑150 (MQ=60)

                                                                                                                          ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                            <  SRR19973074.1619136/150‑1 (MQ=60)

                                                                                                                          ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                            >  SRR19973074.832413/1‑150 (MQ=60)

                                                                                                                           TTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCA                           <  SRR19973074.181464/150‑1 (MQ=60)

                                                                                                                             CATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAA                         >  SRR19973074.3539330/1‑150 (MQ=60)

                                                                                                                                 GGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCG                     <  SRR19973074.2515626/150‑1 (MQ=60)

                                                                                                                                 GGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCG                     <  SRR19973074.2515544/150‑1 (MQ=60)

                                                                                                                                 GGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCG                     <  SRR19973074.2515636/150‑1 (MQ=60)

                                                                                                                                   ATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGC                   >  SRR19973074.3084133/1‑150 (MQ=60)

                                                                                                                                   ATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGC                   >  SRR19973074.2620324/1‑150 (MQ=60)

                                                                                                                                     CGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGT                 <  SRR19973074.1687105/150‑1 (MQ=60)

                                                                                                                                     CGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGT                 >  SRR19973074.916433/1‑150 (MQ=60)

                                                                                                                                     CGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGT                 >  SRR19973074.4101511/1‑150 (MQ=60)

                                                                                                                                      GTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTC                >  SRR19973074.1170334/1‑150 (MQ=60)

                                                                                                                                      GTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTC                <  SRR19973074.1208616/150‑1 (MQ=60)

                                                                                                                                      GTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTC                >  SRR19973074.3265786/1‑150 (MQ=60)

                                                                                                                                      GTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTC                <  SRR19973074.3791888/150‑1 (MQ=60)

                                                                                                                                      GTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTC                <  SRR19973074.3791856/150‑1 (MQ=60)

                                                                                                                                        TAGAGATGTTATAGCTGGAAACCATCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAA              >  SRR19973074.1042983/1‑150 (MQ=60)

                                                                                                                                        TAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAA              >  SRR19973074.1159466/1‑150 (MQ=60)

                                                                                                                                        TAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAA              >  SRR19973074.3482562/1‑150 (MQ=60)

                                                                                                                                         AGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACTTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAT             >  SRR19973074.2894557/1‑150 (MQ=60)

                                                                                                                                         AGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAT             >  SRR19973074.2472904/1‑150 (MQ=60)

                                                                                                                                         AGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAT             >  SRR19973074.2472773/1‑150 (MQ=60)

                                                                                                                                           AGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTT           <  SRR19973074.2646324/150‑1 (MQ=60)

                                                                                                                                           AGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATCT           <  SRR19973074.2646196/150‑1 (MQ=60)

                                                                                                                                            GATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTC          <  SRR19973074.2810420/150‑1 (MQ=60)

                                                                                                                                                TTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCT      <  SRR19973074.2000300/150‑1 (MQ=60)

                                                                                                                                                 TATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT     >  SRR19973074.3844976/1‑150 (MQ=60)

                                                                                                                                                 TATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTT     >  SRR19973074.3844972/1‑150 (MQ=60)

                                                                                                                                                  ATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT    >  SRR19973074.2755173/1‑150 (MQ=60)

                                                                                                                                                    AGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGC  >  SRR19973074.2755921/1‑150 (MQ=60)

TAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGC  >  CP009273/3252110‑3252407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`