Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,252,258 C→A 100% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,252,2580CA99.7% 723.7 / ‑6.0 221D251Y (GAT→TAT) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (118/102);  minor base T (0/1);  total (118/103)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGC  >  CP009273/3252113‑3252402
                                                                                                                                                 |                                                                                                                                                
taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGc                                                                                                                                                >  1:1493336/1‑148 (MQ=255)
taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGc                                                                                                                                                <  1:3419995/148‑1 (MQ=255)
taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGc                                                                                                                                                <  1:2705869/148‑1 (MQ=255)
taaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCAATGACATTCATCGGATCGTTAGAGATGTTATAGc                                                                                                                                                >  2:868550/1‑148 (MQ=255)
 aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCt                                                                                                                                               >  1:2097320/1‑148 (MQ=255)
  aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGTCATTCATCGGATCGTTAGAGATGTTATAGCTg                                                                                                                                              >  1:3336615/1‑148 (MQ=255)
   gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTgg                                                                                                                                             <  1:1263303/148‑1 (MQ=255)
    ccAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGa                                                                                                                                            >  1:3371679/1‑148 (MQ=255)
     cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGaa                                                                                                                                           <  2:142323/148‑1 (MQ=255)
       gTACTGCCGGAATAATGCCGCACGGAGCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAGCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATTGCTGGAAAc                                                                                                                                         <  2:2853726/148‑1 (MQ=255)
         aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCa                                                                                                                                       <  1:1927229/148‑1 (MQ=255)
            gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCt                                                                                                                                    <  2:3507234/148‑1 (MQ=255)
             ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGATGTTATAGCTGGAAACCCGcgg                                                                                                                                   >  1:3262069/1‑146 (MQ=255)
             ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACAGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTg                                                                                                                                   <  2:33135/148‑1 (MQ=255)
              cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTga                                                                                                                                  >  2:2321915/1‑148 (MQ=255)
                 aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacga                                                                                                                               <  1:667425/148‑1 (MQ=255)
                  ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgactac                                                                                                                              >  2:2251692/1‑148 (MQ=255)
                  ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacgac                                                                                                                              >  1:2023432/1‑148 (MQ=255)
                   taatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                             <  2:2722239/148‑1 (MQ=255)
                    aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGc                                                                                                                            >  1:2258391/1‑148 (MQ=255)
                        ccGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcg                                                                                                                        <  2:1519116/148‑1 (MQ=255)
                         cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcgc                                                                                                                       <  2:478733/148‑1 (MQ=255)
                         cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAgcgc                                                                                                                       <  2:630521/148‑1 (MQ=255)
                              gcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacgg                                                                                                                  >  2:1944232/1‑148 (MQ=255)
                                     tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcg                                                                                                           >  1:453982/1‑148 (MQ=255)
                                     tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGgcg                                                                                                           >  1:2220848/1‑148 (MQ=255)
                                            tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGcaca                                                                                                    >  2:446699/1‑148 (MQ=255)
                                                gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGa                                                                                                <  2:1875697/148‑1 (MQ=255)
                                                 ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACCTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAg                                                                                               >  2:2680885/1‑148 (MQ=255)
                                                     cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGAGGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGa                                                                                           >  2:150924/1‑148 (MQ=255)
                                                     cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGGAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGa                                                                                           >  2:2205641/1‑148 (MQ=255)
                                                        gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCAATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCa                                                                                        <  1:868550/148‑1 (MQ=255)
                                                         cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAg                                                                                       <  2:897962/148‑1 (MQ=255)
                                                           cccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGGTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgc                                                                                     >  1:83960/1‑148 (MQ=255)
                                                               ccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgcagca                                                                                 >  2:2513218/1‑148 (MQ=255)
                                                                  gCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTAAACGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACCGCACGGCGCGGCACAATGAGCGGACCAGGCAGCACGc                                                                              >  1:647124/1‑148 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             >  2:1441149/1‑148 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             >  2:1765770/1‑148 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             >  2:2545168/1‑148 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             <  1:355314/148‑1 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             <  1:354852/148‑1 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             >  2:3390322/1‑148 (MQ=255)
                                                                   cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             >  2:372395/1‑148 (MQ=255)
                                                                      cGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTc                                                                          <  2:806642/148‑1 (MQ=255)
                                                                        ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAg                                                                        <  2:2613473/148‑1 (MQ=255)
                                                                         tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgt                                                                       >  2:3230765/1‑148 (MQ=255)
                                                                           tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgtgt                                                                     >  2:1398748/1‑148 (MQ=255)
                                                                           tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgtgt                                                                     <  1:2367642/148‑1 (MQ=255)
                                                                            cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGtt                                                                    >  2:1322128/1‑148 (MQ=255)
                                                                             ttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                   >  2:1985089/1‑148 (MQ=255)
                                                                              tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCa                                                                  <  2:1733434/148‑1 (MQ=255)
                                                                                 cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                               <  1:3144559/148‑1 (MQ=255)
                                                                                  tAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                              >  1:1776191/1‑148 (MQ=255)
                                                                                   aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             >  1:3032731/1‑148 (MQ=255)
                                                                                   aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             <  2:2166351/148‑1 (MQ=255)
                                                                                   aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             >  1:2192152/1‑148 (MQ=255)
                                                                                       gCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGtt                                                         >  2:1402660/1‑148 (MQ=255)
                                                                                        ccAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTc                                                        >  2:783290/1‑148 (MQ=255)
                                                                                           gcgcGTACATGTTGATCCAGTCGATGACATTAATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                     >  2:397745/1‑148 (MQ=255)
                                                                                                tACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTTGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGt                                                >  1:2703296/1‑148 (MQ=255)
                                                                                                tACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGt                                                >  1:3170043/1‑148 (MQ=255)
                                                                                                 aCATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                               >  2:536127/1‑148 (MQ=255)
                                                                                                   aTGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGACACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAt                                             >  1:941039/1‑148 (MQ=255)
                                                                                                    tGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                            <  2:2355432/148‑1 (MQ=255)
                                                                                                    tGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                            >  1:555554/1‑148 (MQ=255)
                                                                                                     gTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGc                                           >  1:3059783/1‑148 (MQ=255)
                                                                                                     gTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGc                                           >  2:2059217/1‑148 (MQ=255)
                                                                                                           ccAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCCTCACt                                     >  2:3418092/1‑148 (MQ=255)
                                                                                                           ccAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACt                                     <  2:2492293/148‑1 (MQ=255)
                                                                                                             aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                   <  2:3253416/148‑1 (MQ=255)
                                                                                                             aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                   <  2:9007/148‑1 (MQ=255)
                                                                                                             aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                   >  2:1994992/1‑148 (MQ=255)
                                                                                                              gTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGc                                  <  1:291434/148‑1 (MQ=255)
                                                                                                                  aTGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                              >  1:1013359/1‑148 (MQ=255)
                                                                                                                  aTGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                              <  2:3183672/148‑1 (MQ=255)
                                                                                                                  aTGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGa                              <  2:176452/148‑1 (MQ=255)
                                                                                                                   tGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                             >  1:1541834/1‑148 (MQ=255)
                                                                                                                    gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATa                            <  1:3323346/148‑1 (MQ=255)
                                                                                                                      cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                          >  2:1263885/1‑148 (MQ=255)
                                                                                                                      cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                          <  1:1615199/148‑1 (MQ=255)
                                                                                                                      cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                          <  2:2558605/148‑1 (MQ=255)
                                                                                                                       aTTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACgg                         <  1:1941123/148‑1 (MQ=255)
                                                                                                                        ttCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACggg                        <  2:1641791/148‑1 (MQ=255)
                                                                                                                         tCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                       >  1:1217778/1‑148 (MQ=255)
                                                                                                                           aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaa                     >  1:2154642/1‑148 (MQ=255)
                                                                                                                           aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaa                     >  1:2442530/1‑148 (MQ=255)
                                                                                                                              ggATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAAc                  >  1:2419444/1‑148 (MQ=255)
                                                                                                                               gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAAcc                 <  2:648155/148‑1 (MQ=255)
                                                                                                                                aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCg                >  2:2578783/1‑148 (MQ=255)
                                                                                                                                 tCGTTAGAGATGTTATAGCTGGAAATCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg               <  2:1166079/148‑1 (MQ=255)
                                                                                                                                 tCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTTATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg               >  2:2743129/1‑148 (MQ=255)
                                                                                                                                 tCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCgg               >  1:3080525/1‑148 (MQ=255)
                                                                                                                                  cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc              >  2:190768/1‑148 (MQ=255)
                                                                                                                                  cGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGc              >  1:1149743/1‑148 (MQ=255)
                                                                                                                                    ttAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGt            <  2:607118/148‑1 (MQ=255)
                                                                                                                                       gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa         >  1:679617/1‑148 (MQ=255)
                                                                                                                                         gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt       <  1:107163/148‑1 (MQ=255)
                                                                                                                                         gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt       >  2:2098559/1‑148 (MQ=255)
                                                                                                                                             ttATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAGTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGGCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCg   <  2:1067448/148‑1 (MQ=255)
                                                                                                                                              tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGc  >  1:1858915/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGC  >  CP009273/3252113‑3252402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT  >  CP009273/3252109‑3252405
                                                                                                                                                     |                                                                                                                                                   
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACCTTCATCGGATCGGTAGCGATGTTATA                                                                                                                                                     >  SRR19973065.354468/1‑150 (MQ=60)
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATA                                                                                                                                                     >  SRR19973065.2137320/1‑150 (MQ=60)
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTGAGAGATGTTATA                                                                                                                                                     >  SRR19973065.631723/1‑150 (MQ=60)
  AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGC                                                                                                                                                   >  SRR19973065.1502924/1‑150 (MQ=60)
   ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCT                                                                                                                                                  <  SRR19973065.1016915/150‑1 (MQ=60)
   ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCT                                                                                                                                                  >  SRR19973065.2110810/1‑150 (MQ=60)
   ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCT                                                                                                                                                  <  SRR19973065.2671226/150‑1 (MQ=60)
    TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGTCATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                 >  SRR19973065.3356986/1‑150 (MQ=60)
    TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                 <  SRR19973065.3440890/150‑1 (MQ=60)
    TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                 <  SRR19973065.3440878/150‑1 (MQ=60)
    TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                 <  SRR19973065.2722691/150‑1 (MQ=60)
    TAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTG                                                                                                                                                 <  SRR19973065.2722640/150‑1 (MQ=60)
     AAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGG                                                                                                                                                <  SRR19973065.903370/150‑1 (MQ=60)
     AAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGG                                                                                                                                                <  SRR19973065.2672654/150‑1 (MQ=60)
      AGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATATCTGGA                                                                                                                                               >  SRR19973065.3392294/1‑150 (MQ=60)
      AGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGA                                                                                                                                               <  SRR19973065.2550138/150‑1 (MQ=60)
      AGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGA                                                                                                                                               >  SRR19973065.3392297/1‑150 (MQ=60)
       GCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAA                                                                                                                                              <  SRR19973065.1271626/150‑1 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGTCATTCATCGGATCGTTAGGGATGTTATAGCTGGAAACC                                                                                                                                           >  SRR19973065.652418/1‑150 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGTCATTCATCGGATCGTTAGAGATGTTATAGCTGGAAATC                                                                                                                                           >  SRR19973065.636957/1‑150 (MQ=60)
          AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGTGATGTTATATCTGGAAATC                                                                                                                                           >  SRR19973065.1173524/1‑150 (MQ=60)
            TACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAG                                                                                                                                         <  SRR19973065.156698/150‑1 (MQ=60)
             ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                        <  SRR19973065.1939856/150‑1 (MQ=60)
             ACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGC                                                                                                                                        <  SRR19973065.1941174/150‑1 (MQ=60)
              CTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCT                                                                                                                                       <  SRR19973065.1128912/150‑1 (MQ=60)
               TGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGATGTTATAGCTGGAAACCcgcgg                                                                                                                                      >  SRR19973065.3281827/1‑145 (MQ=60)
                 CCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGAC                                                                                                                                    <  SRR19973065.2140006/150‑1 (MQ=60)
                    GAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGAC                                                                                                                                 >  SRR19973065.2036443/1‑150 (MQ=60)
                     AATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACG                                                                                                                                <  SRR19973065.671868/150‑1 (MQ=60)
                      ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGC                                                                                                                               >  SRR19973065.2272679/1‑150 (MQ=60)
                      ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGC                                                                                                                               >  SRR19973065.2271623/1‑150 (MQ=60)
                               CACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTGTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTGAGGGATGGTATAGCTGGCAACCAGCGGACGACGCAGCGCGACG                                                                                                                      >  SRR19973065.3028420/1‑150 (MQ=60)
                                   CACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCAC                                                                                                                  >  SRR19973065.3493788/1‑150 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                              >  SRR19973065.457124/1‑150 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                              >  SRR19973065.2235020/1‑150 (MQ=60)
                                       GTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCG                                                                                                              >  SRR19973065.2235143/1‑150 (MQ=60)
                                               GTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAT                                                                                                      >  SRR19973065.785019/1‑150 (MQ=60)
                                               GTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACAGACAGCGCGTACATGTTTATCAAGTCGATTACATTCATCGGATCGTTAGAGAATTTATATCTGGAAACCACATGACGTCGCAGCCCAACGGCACGGCGCGCCCCAT                                                                                                      >  SRR19973065.3365436/1‑150 (MQ=60)
                                                TGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATT                                                                                                     <  SRR19973065.2074532/150‑1 (MQ=60)
                                                TGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATT                                                                                                     <  SRR19973065.1935682/150‑1 (MQ=60)
                                                    GTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGC                                                                                                 <  SRR19973065.677432/150‑1 (MQ=60)
                                                     TTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCG                                                                                                <  SRR19973065.223503/150‑1 (MQ=60)
                                                            GCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCAATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGG                                                                                         <  SRR19973065.874118/150‑1 (MQ=60)
                                                            GCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCAATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGG                                                                                         <  SRR19973065.871983/150‑1 (MQ=60)
                                                             CGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGC                                                                                        >  SRR19973065.84862/1‑150 (MQ=60)
                                                             CGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGGTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGC                                                                                        >  SRR19973065.84781/1‑150 (MQ=60)
                                                              GCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCA                                                                                       <  SRR19973065.1547640/150‑1 (MQ=60)
                                                              GCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCA                                                                                       <  SRR19973065.3298478/150‑1 (MQ=60)
                                                                    CAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTAAACGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACCGCACGGCGCGGCACAATGAGCGGACCAGGCAGCACGC                                                                                 >  SRR19973065.651380/1‑150 (MQ=60)
                                                                    CAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAATAATCGGATCGGTAGAGATGTTATATCTGGAAAACAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAATGAGCGGACCAGGCAGCACGC                                                                                 >  SRR19973065.651379/1‑150 (MQ=60)
                                                                       CTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                              <  SRR19973065.357815/150‑1 (MQ=60)
                                                                       CTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                              <  SRR19973065.357384/150‑1 (MQ=60)
                                                                       CTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTT                                                                              <  SRR19973065.357349/150‑1 (MQ=60)
                                                                        TGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACCTTGAGCGGCCCAGGCAGCACGCCTTC                                                                             >  SRR19973065.2425889/1‑150 (MQ=60)
                                                                               TCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTC                                                                      <  SRR19973065.2382397/150‑1 (MQ=60)
                                                                                 TTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAT                                                                    <  SRR19973065.103653/150‑1 (MQ=60)
                                                                                   CACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCC                                                                  >  SRR19973065.2296226/1‑150 (MQ=60)
                                                                                    ACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCC                                                                 <  SRR19973065.1997705/150‑1 (MQ=60)
                                                                                    ACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCC                                                                 >  SRR19973065.1787804/1‑150 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                >  SRR19973065.2206208/1‑150 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                <  SRR19973065.3119439/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                <  SRR19973065.3163660/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                >  SRR19973065.3051307/1‑150 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                <  SRR19973065.1407218/150‑1 (MQ=60)
                                                                                     CTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCA                                                                >  SRR19973065.3053483/1‑150 (MQ=60)
                                                                                      TAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCANCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCAC                                                               >  SRR19973065.1796287/1‑150 (MQ=60)
                                                                                      TAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCAC                                                               >  SRR19973065.498339/1‑150 (MQ=60)
                                                                                       AACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACG                                                              <  SRR19973065.2742318/150‑1 (MQ=60)
                                                                                                  CGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTTGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGT                                                   >  SRR19973065.2720100/1‑150 (MQ=60)
                                                                                                  CGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGT                                                   >  SRR19973065.3189228/1‑150 (MQ=60)
                                                                                                   GTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTC                                                  >  SRR19973065.1229037/1‑150 (MQ=60)
                                                                                                     ACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGACACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCAT                                                >  SRR19973065.947253/1‑150 (MQ=60)
                                                                                                      CATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATG                                               <  SRR19973065.1728975/150‑1 (MQ=60)
                                                                                                      CATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATG                                               >  SRR19973065.559106/1‑150 (MQ=60)
                                                                                                       ATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGC                                              >  SRR19973065.3078491/1‑150 (MQ=60)
                                                                                                       ATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGC                                              >  SRR19973065.3078364/1‑150 (MQ=60)
                                                                                                             ATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACT                                        <  SRR19973065.3532661/150‑1 (MQ=60)
                                                                                                                  GTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCA                                   <  SRR19973065.293326/150‑1 (MQ=60)
                                                                                                                    CGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGA                                 >  SRR19973065.1020074/1‑150 (MQ=60)
                                                                                                                     GATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAT                                >  SRR19973065.1552029/1‑150 (MQ=60)
                                                                                                                        GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                             <  SRR19973065.3343624/150‑1 (MQ=60)
                                                                                                                        GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                             <  SRR19973065.3343602/150‑1 (MQ=60)
                                                                                                                        GACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACG                             <  SRR19973065.3343977/150‑1 (MQ=60)
                                                                                                                          CATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGG                           <  SRR19973065.37006/150‑1 (MQ=60)
                                                                                                                          CATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGG                           <  SRR19973065.1626038/150‑1 (MQ=60)
                                                                                                                           ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                          >  SRR19973065.1225685/1‑150 (MQ=60)
                                                                                                                           ATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGC                          <  SRR19973065.1953820/150‑1 (MQ=60)
                                                                                                                            TTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCA                         >  SRR19973065.20598/1‑150 (MQ=60)
                                                                                                                             TCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAA                        >  SRR19973065.2168499/1‑150 (MQ=60)
                                                                                                                             TCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAA                        >  SRR19973065.2457926/1‑150 (MQ=60)
                                                                                                                               ATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAA                      >  SRR19973065.3185690/1‑150 (MQ=60)
                                                                                                                                TCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAAC                     >  SRR19973065.2434698/1‑150 (MQ=60)
                                                                                                                                   GATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGG                  >  SRR19973065.3099324/1‑150 (MQ=60)
                                                                                                                                   GATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGG                  >  SRR19973065.3099337/1‑150 (MQ=60)
                                                                                                                                    ATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGC                 >  SRR19973065.1157151/1‑150 (MQ=60)
                                                                                                                                         TAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAA            >  SRR19973065.2456052/1‑150 (MQ=60)
                                                                                                                                         TAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAA            >  SRR19973065.684142/1‑150 (MQ=60)
                                                                                                                                            AGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTT         >  SRR19973065.956317/1‑150 (MQ=60)
                                                                                                                                             GATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTC        <  SRR19973065.2200129/150‑1 (MQ=60)
                                                                                                                                             GATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTC        <  SRR19973065.108139/150‑1 (MQ=60)
                                                                                                                                              ATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGNCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCC       >  SRR19973065.1791836/1‑150 (MQ=60)
                                                                                                                                                GTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGC     >  SRR19973065.1871145/1‑150 (MQ=60)
                                                                                                                                                GTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATCTCCGC     >  SRR19973065.1273948/1‑150 (MQ=60)
                                                                                                                                                   ATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT  <  SRR19973065.993983/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTT  >  CP009273/3252109‑3252405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑