Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,336 G→T 28.3% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,3360GT28.3% 755.9 / 302.5 351Q100K (CAG→AAG) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (129/122);  new base T (43/57);  total (172/179)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA  >  CP009273/435189‑435480
                                                                                                                                                   |                                                                                                                                                
    gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGTCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGa                                                                                                                                             <  2:1317450/149‑1 (MQ=255)
    gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGa                                                                                                                                             >  2:2369621/1‑149 (MQ=255)
          gcggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAAATCCGGCACTGATGGAGGTAGATGAATGCCCGACGCTAAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGc                                                                                                                                       >  2:3035225/1‑149 (MQ=255)
           cggcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGcc                                                                                                                                      <  1:388432/149‑1 (MQ=255)
                aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAttt                                                                                                                                 <  1:5033745/149‑1 (MQ=255)
                       ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTcgcg                                                                                                                          >  1:1505601/1‑149 (MQ=255)
                       ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTcgcg                                                                                                                          >  1:381609/1‑149 (MQ=255)
                        cttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCgcgg                                                                                                                         >  2:5181282/1‑149 (MQ=255)
                         ttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCgcggc                                                                                                                        >  1:4147854/1‑149 (MQ=255)
                             ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTGTGTCGCGGCGTcc                                                                                                                    >  1:4795355/1‑149 (MQ=255)
                              tttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCg                                                                                                                   <  1:2210972/149‑1 (MQ=255)
                              tttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCg                                                                                                                   >  2:5014740/1‑149 (MQ=255)
                                 cGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTc                                                                                                                <  2:4534549/149‑1 (MQ=255)
                                  ggCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCa                                                                                                               >  2:4963448/1‑149 (MQ=255)
                                  ggCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCa                                                                                                               >  1:4054814/1‑149 (MQ=255)
                                   gcagcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaa                                                                                                              >  1:3996874/1‑149 (MQ=255)
                                   gcagcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaa                                                                                                              >  1:2524778/1‑149 (MQ=255)
                                      gcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAt                                                                                                           <  2:588929/149‑1 (MQ=255)
                                        aaCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAttt                                                                                                         <  1:82601/149‑1 (MQ=255)
                                        aaCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAttt                                                                                                         >  1:4411898/1‑149 (MQ=255)
                                        aaCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAttt                                                                                                         <  2:780753/149‑1 (MQ=255)
                                         aCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAtttt                                                                                                        >  2:1382909/1‑149 (MQ=255)
                                          ccGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTa                                                                                                       <  1:1731619/149‑1 (MQ=255)
                                          ccGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTa                                                                                                       <  2:3394726/149‑1 (MQ=255)
                                              aaTACCAATTCCGGCACGGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCg                                                                                                   <  2:3672345/149‑1 (MQ=255)
                                                 aCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGAt                                                                                                >  1:473242/1‑149 (MQ=255)
                                                    aaTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             >  2:4934390/1‑149 (MQ=255)
                                                      ttCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGcc                                                                                           >  1:2694232/1‑149 (MQ=255)
                                                       tCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCt                                                                                          <  2:493340/149‑1 (MQ=255)
                                                         cGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGa                                                                                        <  2:4692383/149‑1 (MQ=255)
                                                           gCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGTAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:3029781/1‑149 (MQ=255)
                                                           gCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:772274/1‑149 (MQ=255)
                                                           gCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:2603839/1‑149 (MQ=255)
                                                             aCTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                    >  2:1099743/1‑149 (MQ=255)
                                                             aCTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                    >  2:4397502/1‑149 (MQ=255)
                                                                  tGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                               >  1:3502239/1‑149 (MQ=255)
                                                                   ggAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                              >  2:167950/1‑149 (MQ=255)
                                                                     aGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATcc                                                                            <  2:1284634/149‑1 (MQ=255)
                                                                        ttGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaa                                                                         >  2:1501762/1‑149 (MQ=255)
                                                                         tgatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaaa                                                                        <  2:4839796/149‑1 (MQ=255)
                                                                         tgatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaaa                                                                        >  2:1912328/1‑149 (MQ=255)
                                                                          gatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAAt                                                                       >  2:1745579/1‑149 (MQ=255)
                                                                           atgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATc                                                                      <  1:3247928/149‑1 (MQ=255)
                                                                           atgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATc                                                                      <  1:711021/149‑1 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                    >  1:428122/1‑149 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                    <  2:2684380/149‑1 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                    <  2:2352330/149‑1 (MQ=255)
                                                                             gaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCaa                                                                    <  1:4239878/149‑1 (MQ=255)
                                                                              aaTGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAt                                                                   >  2:3271933/1‑149 (MQ=255)
                                                                              aaTGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAt                                                                   >  2:2850243/1‑149 (MQ=255)
                                                                                tGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTg                                                                 >  1:1059774/1‑149 (MQ=255)
                                                                                   ccGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTc                                                              >  1:3279600/1‑149 (MQ=255)
                                                                                   ccGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTc                                                              <  1:3989052/149‑1 (MQ=255)
                                                                                     gACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaa                                                            >  2:4153129/1‑149 (MQ=255)
                                                                                      aCGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaaa                                                           <  1:1590488/149‑1 (MQ=255)
                                                                                       cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAAc                                                          <  2:153183/149‑1 (MQ=255)
                                                                                        gCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                         >  2:2561041/1‑149 (MQ=255)
                                                                                         cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgg                                                        <  1:3083799/149‑1 (MQ=255)
                                                                                           tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgggg                                                      >  1:840203/1‑149 (MQ=255)
                                                                                           tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACgggg                                                      <  2:4806702/149‑1 (MQ=255)
                                                                                            aaTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgt                                                     <  1:938397/149‑1 (MQ=255)
                                                                                             aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                    <  2:5074939/149‑1 (MQ=255)
                                                                                             aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                    >  1:3625875/1‑149 (MQ=255)
                                                                                              tACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGTGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtgt                                                   <  2:3873707/149‑1 (MQ=255)
                                                                                               aCGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgtt                                                  >  2:1006090/1‑149 (MQ=255)
                                                                                                 gTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgttgt                                                <  2:4191885/149‑1 (MQ=255)
                                                                                                 gTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGtgttgt                                                >  2:3513769/1‑149 (MQ=255)
                                                                                                     tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                            >  1:3403692/1‑149 (MQ=255)
                                                                                                      atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                           <  1:2166786/149‑1 (MQ=255)
                                                                                                      atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                           <  1:2326027/149‑1 (MQ=255)
                                                                                                      atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                           >  2:5038741/1‑149 (MQ=255)
                                                                                                      atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACa                                           >  1:2884261/1‑149 (MQ=255)
                                                                                                       ttCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCCAATCAATTGGTCAAACGGGGTGTTGTAGACAt                                          >  2:2221337/1‑149 (MQ=255)
                                                                                                       ttCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACAt                                          >  1:4435254/1‑149 (MQ=255)
                                                                                                       ttCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACAt                                          <  1:521599/149‑1 (MQ=255)
                                                                                                        tCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATa                                         <  2:1784969/149‑1 (MQ=255)
                                                                                                          gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGt                                       <  1:1826884/149‑1 (MQ=255)
                                                                                                           cTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                      >  1:1023992/1‑149 (MQ=255)
                                                                                                               cgccgcGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAgc                                  <  1:1426367/149‑1 (MQ=255)
                                                                                                                   gcgcCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                              <  1:3556527/149‑1 (MQ=255)
                                                                                                                   gcgcCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                              >  2:5141484/1‑149 (MQ=255)
                                                                                                                    cgcCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCAc                             <  1:4943236/149‑1 (MQ=255)
                                                                                                                      ccACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACgg                           <  1:3602204/149‑1 (MQ=255)
                                                                                                                        aCGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                         <  2:3996874/149‑1 (MQ=255)
                                                                                                                         cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCa                        >  2:2065290/1‑149 (MQ=255)
                                                                                                                            gAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGtt                     <  1:1804202/149‑1 (MQ=255)
                                                                                                                             aaCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTc                    >  1:1860664/1‑149 (MQ=255)
                                                                                                                             aaCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTc                    <  1:3314744/149‑1 (MQ=255)
                                                                                                                                gggTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGAc                 <  1:4676747/149‑1 (MQ=255)
                                                                                                                                gggTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGAc                 <  1:2422620/149‑1 (MQ=255)
                                                                                                                                  gTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCg               <  2:910879/149‑1 (MQ=255)
                                                                                                                                       gACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGccc          >  2:2298270/1‑149 (MQ=255)
                                                                                                                                       gACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAACTTTTATGCGGATAAGCCTGATGCCCCACCTCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGccc          <  2:1280288/149‑1 (MQ=255)
                                                                                                                                           gCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGcc      >  2:16992/1‑149 (MQ=255)
                                                                                                                                            ccTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGccc     >  2:2440743/1‑149 (MQ=255)
                                                                                                                                            ccTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGccc     >  2:3446853/1‑149 (MQ=255)
                                                                                                                                             cTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCg    >  2:2411416/1‑149 (MQ=255)
                                                                                                                                             cTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCg    >  2:3242939/1‑149 (MQ=255)
                                                                                                                                              tttCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg   >  1:1372819/1‑149 (MQ=255)
                                                                                                                                               ttCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGa  <  2:4679206/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA  >  CP009273/435189‑435480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A