Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,336 G→T 100% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,3360GT99.5% 650.9 / ‑4.1 199Q100K (CAG→AAG) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (112/86);  total (112/87)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

CAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA  >  CP009273/435191‑435480
                                                                                                                                                 |                                                                                                                                                
caGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTAc                                                                                                                                                >  1:246651/1‑148 (MQ=255)
caGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGGTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTAc                                                                                                                                                >  2:1012738/1‑148 (MQ=255)
 aGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACg                                                                                                                                               >  2:511010/1‑148 (MQ=255)
  gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACgg                                                                                                                                              <  2:1329916/148‑1 (MQ=255)
  gACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACgg                                                                                                                                              <  2:1453229/148‑1 (MQ=255)
   aCGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGa                                                                                                                                             <  1:452301/148‑1 (MQ=255)
      gTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATgg                                                                                                                                          <  1:2992964/148‑1 (MQ=255)
      gTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATgg                                                                                                                                          <  2:935527/148‑1 (MQ=255)
            cgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAAGTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGa                                                                                                                                    <  2:2384597/148‑1 (MQ=255)
              aTTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtt                                                                                                                                  <  2:291437/148‑1 (MQ=255)
                ttttGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGAtttt                                                                                                                                >  2:1306263/1‑148 (MQ=255)
                    gCCTTCTTTGTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcg                                                                                                                            <  2:660555/148‑1 (MQ=255)
                     ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                           >  2:393489/1‑148 (MQ=255)
                     ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                           >  2:3283683/1‑148 (MQ=255)
                     ccTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTcgc                                                                                                                           >  2:3035794/1‑148 (MQ=255)
                       ttcttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcgg                                                                                                                         <  1:2519545/148‑1 (MQ=255)
                         cttTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCgcggcg                                                                                                                       >  2:3023169/1‑148 (MQ=255)
                           ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTc                                                                                                                     >  1:3446511/1‑148 (MQ=255)
                                    gcaACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCaaaa                                                                                                            >  2:231130/1‑148 (MQ=255)
                                     caACCGCAATACCAAGTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAt                                                                                                           <  1:2811222/148‑1 (MQ=255)
                                      aaCCGCAATAACAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACTCTTAATAAGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCTCGGCGTCCGGTCAAAAtt                                                                                                          <  1:2468882/148‑1 (MQ=255)
                                       aCCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAttt                                                                                                         >  1:3179944/1‑148 (MQ=255)
                                        ccGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAtttt                                                                                                        >  2:3248177/1‑148 (MQ=255)
                                          gCAATACCACTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTAt                                                                                                      <  2:2896082/148‑1 (MQ=255)
                                           cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                     <  2:4483/148‑1 (MQ=255)
                                           cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                     >  1:2026094/1‑148 (MQ=255)
                                           cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                     >  2:1791350/1‑148 (MQ=255)
                                           cAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATg                                                                                                     <  2:707164/148‑1 (MQ=255)
                                             aTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCg                                                                                                   >  2:2732525/1‑148 (MQ=255)
                                             aTACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCg                                                                                                   >  2:863742/1‑148 (MQ=255)
                                               aCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGTGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGAGGa                                                                                                 >  2:803267/1‑148 (MQ=255)
                                                  aaTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATaa                                                                                              <  1:2674039/148‑1 (MQ=255)
                                                   agtCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             <  2:1518623/146‑1 (MQ=255)
                                                   aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             <  2:1003289/148‑1 (MQ=255)
                                                   aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             <  2:1003158/148‑1 (MQ=255)
                                                   aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             >  2:2546466/1‑148 (MQ=255)
                                                   aTTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAg                                                                                             <  2:429682/148‑1 (MQ=255)
                                                    ttCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGc                                                                                            >  2:52456/1‑148 (MQ=255)
                                                     tCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGcc                                                                                           >  2:124577/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:807569/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:1001776/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:1942659/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:1001660/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      >  1:807086/1‑148 (MQ=255)
                                                          cACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATAGTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATg                                                                                      <  2:1953169/148‑1 (MQ=255)
                                                            cTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGcc                                                                                    <  2:786558/148‑1 (MQ=255)
                                                                 ggAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCcaca                                                                               <  1:858047/148‑1 (MQ=255)
                                                                  gAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                              <  1:2150479/148‑1 (MQ=255)
                                                                  gAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAt                                                                              >  1:1840119/1‑148 (MQ=255)
                                                                     gTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATccc                                                                           >  2:307843/1‑148 (MQ=255)
                                                                     gTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATccc                                                                           >  2:308294/1‑148 (MQ=255)
                                                                      ttGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCa                                                                          >  1:526567/1‑148 (MQ=255)
                                                                        gatgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTGTCTTACGGATGTTGCCGATTTTTTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCaaa                                                                        >  1:3246141/1‑148 (MQ=255)
                                                                         atgaATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAAt                                                                       >  2:1340415/1‑148 (MQ=255)
                                                                             aTGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAt                                                                   >  1:1917411/1‑148 (MQ=255)
                                                                                cccGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTgg                                                                >  2:3493527/1‑148 (MQ=255)
                                                                                  cGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTc                                                              >  2:2677491/1‑148 (MQ=255)
                                                                                   gACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCa                                                             >  2:2594382/1‑148 (MQ=255)
                                                                                     cGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTTAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCaaa                                                           <  2:1151285/148‑1 (MQ=255)
                                                                                       cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                         >  1:1103826/1‑148 (MQ=255)
                                                                                       cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACg                                                         <  2:836565/148‑1 (MQ=255)
                                                                                       cTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTACGGTCAAAATTTTATGCGGATAAGCCTTATGCCCCACATCCCAAATCAATTGGTCAAACg                                                         <  1:1306263/148‑1 (MQ=255)
                                                                                         tAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACggg                                                       <  2:1664636/148‑1 (MQ=255)
                                                                                           aTACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgt                                                     >  1:866723/1‑148 (MQ=255)
                                                                                            tACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtg                                                    >  2:2491380/1‑148 (MQ=255)
                                                                                             aCGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGgtgt                                                   <  1:2392591/148‑1 (MQ=255)
                                                                                                 cATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTa                                               >  2:864894/1‑148 (MQ=255)
                                                                                                 cATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTa                                               >  2:27605/1‑148 (MQ=255)
                                                                                                  atatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAg                                              <  1:1658774/148‑1 (MQ=255)
                                                                                                   tatTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGa                                             >  1:662481/1‑148 (MQ=255)
                                                                                                    atTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAc                                            >  1:3040146/1‑148 (MQ=255)
                                                                                                       cGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATa                                         <  2:1093210/148‑1 (MQ=255)
                                                                                                       cGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATa                                         <  2:2872025/148‑1 (MQ=255)
                                                                                                        gCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAg                                        <  1:1042060/148‑1 (MQ=255)
                                                                                                          tttCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTg                                      >  1:992162/1‑148 (MQ=255)
                                                                                                            tCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCa                                    >  2:2124291/1‑148 (MQ=255)
                                                                                                             cgccgcGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAg                                   <  1:1011696/148‑1 (MQ=255)
                                                                                                             cgccgcGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAg                                   <  1:2790055/148‑1 (MQ=255)
                                                                                                              gccgcGCCACGGGAACGGGTGCAGACAGCGTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAGTTTATGCGGATAAGACGGATGCCCCACAACCCAAATCAATTGGTCAAACGGGGTGTTGTTGACATAGTGCAgc                                  >  1:1400938/1‑148 (MQ=255)
                                                                                                                cgcgCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAgcgc                                <  1:124577/148‑1 (MQ=255)
                                                                                                                  cgcCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCa                              <  1:1359077/148‑1 (MQ=255)
                                                                                                                       cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                         <  1:3489108/148‑1 (MQ=255)
                                                                                                                       cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                         <  1:1587108/148‑1 (MQ=255)
                                                                                                                       cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                         >  2:3090139/1‑148 (MQ=255)
                                                                                                                       cGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTc                         >  1:2275876/1‑148 (MQ=255)
                                                                                                                         ggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                       >  1:1993629/1‑148 (MQ=255)
                                                                                                                         ggAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAg                       >  1:1345488/1‑148 (MQ=255)
                                                                                                                          gAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGt                      <  2:2844459/148‑1 (MQ=255)
                                                                                                                           aaCGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGtt                     <  2:1840119/148‑1 (MQ=255)
                                                                                                                                  gCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTTGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGt              >  2:604555/1‑148 (MQ=255)
                                                                                                                                      aCCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGccc          >  1:31089/1‑148 (MQ=255)
                                                                                                                                          ccTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGcc      >  1:3053719/1‑148 (MQ=255)
                                                                                                                                          ccTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGcc      >  2:2127383/1‑148 (MQ=255)
                                                                                                                                          ccTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGcc      >  2:371299/1‑148 (MQ=255)
                                                                                                                                          ccTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGGTGTAGACATAGAGCAGCGCCACGGTCAGTTCGACCGTGCCCAGcc      >  2:370635/1‑148 (MQ=255)
                                                                                                                                            tttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCg    >  2:49841/1‑148 (MQ=255)
                                                                                                                                             ttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg   >  2:1846822/1‑148 (MQ=255)
                                                                                                                                             ttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg   >  1:2330596/1‑148 (MQ=255)
                                                                                                                                             ttCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCgg   <  1:863742/148‑1 (MQ=255)
                                                                                                                                              tCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGa  >  1:3208617/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA  >  CP009273/435191‑435480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGGC  >  CP009273/435187‑435483
                                                                                                                                                     |                                                                                                                                                   
GACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTT                                                                                                                                                     <  SRR19973066.1586585/150‑1 (MQ=60)
  CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTAC                                                                                                                                                   >  SRR19973066.248561/1‑150 (MQ=60)
  CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTAC                                                                                                                                                   >  SRR19973066.248620/1‑150 (MQ=60)
  CACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTAC                                                                                                                                                   >  SRR19973066.249126/1‑150 (MQ=60)
   ACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACG                                                                                                                                                  <  SRR19973066.1049269/150‑1 (MQ=60)
      GACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGAT                                                                                                                                               <  SRR19973066.3162277/150‑1 (MQ=60)
      GACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGAT                                                                                                                                               <  SRR19973066.1328316/150‑1 (MQ=60)
       ACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATG                                                                                                                                              <  SRR19973066.455778/150‑1 (MQ=60)
          GTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTG                                                                                                                                           <  SRR19973066.3012800/150‑1 (MQ=60)
          GTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTG                                                                                                                                           <  SRR19973066.3012810/150‑1 (MQ=60)
                           TTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCG                                                                                                                          <  SRR19973066.2536679/150‑1 (MQ=60)
                             CTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTC                                                                                                                        >  SRR19973066.3469561/1‑150 (MQ=60)
                                TTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGG                                                                                                                     <  SRR19973066.996170/150‑1 (MQ=60)
                                       AGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAT                                                                                                              >  SRR19973066.3210021/1‑150 (MQ=60)
                                       AGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAT                                                                                                              >  SRR19973066.809988/1‑150 (MQ=60)
                                         CAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTT                                                                                                            >  SRR19973066.3201103/1‑150 (MQ=60)
                                         CAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTT                                                                                                            <  SRR19973066.2473315/150‑1 (MQ=60)
                                         CAACCGCAATACCAAGTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTT                                                                                                            <  SRR19973066.2830131/150‑1 (MQ=60)
                                          AACCGCAATAACAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACTCTTAATAAGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCTCGGCGTCCGGTCAAAATTTT                                                                                                           <  SRR19973066.2485718/150‑1 (MQ=60)
                                            CCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTAT                                                                                                         <  SRR19973066.210793/150‑1 (MQ=60)
                                             CGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATG                                                                                                        >  SRR19973066.2040252/1‑150 (MQ=60)
                                                 ATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGA                                                                                                    >  SRR19973066.3094293/1‑150 (MQ=60)
                                                      AATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGC                                                                                               <  SRR19973066.2691905/150‑1 (MQ=60)
                                                        TTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCT                                                                                             <  SRR19973066.126844/150‑1 (MQ=60)
                                                        TTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCT                                                                                             <  SRR19973066.125756/150‑1 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.813014/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.813020/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.813498/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.1009161/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.1956430/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.1009165/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.1009045/1‑150 (MQ=60)
                                                            GGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATG                                                                                         >  SRR19973066.1956242/1‑150 (MQ=60)
                                                              CACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCC                                                                                       <  SRR19973066.2199371/150‑1 (MQ=60)
                                                                    TGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACAT                                                                                 >  SRR19973066.1853269/1‑150 (MQ=60)
                                                                     GGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATC                                                                                <  SRR19973066.864231/150‑1 (MQ=60)
                                                                      GAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCC                                                                               <  SRR19973066.2165408/150‑1 (MQ=60)
                                                                       AGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCC                                                                              <  SRR19973066.2875569/150‑1 (MQ=60)
                                                                        GGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCA                                                                             >  SRR19973066.530426/1‑150 (MQ=60)
                                                                          TTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTGTCTTACGGATGTTGCCGATTTTTTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAA                                                                           >  SRR19973066.3267541/1‑150 (MQ=60)
                                                                              TGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGAGGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAA                                                                       >  SRR19973066.1776630/1‑150 (MQ=60)
                                                                               GNATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAT                                                                      >  SRR19973066.1930979/1‑150 (MQ=60)
                                                                               GAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAAT                                                                      >  SRR19973066.1931055/1‑150 (MQ=60)
                                                                                       GACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAA                                                              >  SRR19973066.3397141/1‑150 (MQ=60)
                                                                                       GACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAA                                                              >  SRR19973066.1619479/1‑150 (MQ=60)
                                                                                        ACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCTCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAAC                                                             >  SRR19973066.2815715/1‑150 (MQ=60)
                                                                                         CGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACG                                                            >  SRR19973066.1111831/1‑150 (MQ=60)
                                                                                           CTTAATACGTCATATTCGCTTTCGCCGCGCCACTGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTACGGTCAAAATTTTATGCGGATAAGCCTTATGCCCCACATCCCAAATCAATTGGTCAAACGGG                                                          <  SRR19973066.1315735/150‑1 (MQ=60)
                                                                                             TAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGT                                                        >  SRR19973066.2676060/1‑150 (MQ=60)
                                                                                             TAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGT                                                        >  SRR19973066.452372/1‑150 (MQ=60)
                                                                                             TAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGT                                                        >  SRR19973066.2363145/1‑150 (MQ=60)
                                                                                             TAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGT                                                        >  SRR19973066.872941/1‑150 (MQ=60)
                                                                                             TAATACGTCATAGTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGT                                                        <  SRR19973066.87539/150‑1 (MQ=60)
                                                                                               ATACGTCATATTCGATTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGT                                                      >  SRR19973066.2034600/1‑150 (MQ=60)
                                                                                                 ACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTG                                                    <  SRR19973066.2408699/150‑1 (MQ=60)
                                                                                                 ACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTG                                                    >  SRR19973066.518794/1‑150 (MQ=60)
                                                                                                     CATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGA                                                >  SRR19973066.2725682/1‑150 (MQ=60)
                                                                                                     CATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGA                                                >  SRR19973066.667319/1‑150 (MQ=60)
                                                                                                      ATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAC                                               <  SRR19973066.1670861/150‑1 (MQ=60)
                                                                                                      ATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAC                                               >  SRR19973066.3060463/1‑150 (MQ=60)
                                                                                                      ATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGAC                                               <  SRR19973066.1020196/150‑1 (MQ=60)
                                                                                                            GCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTG                                         >  SRR19973066.999869/1‑150 (MQ=60)
                                                                                                            GCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTG                                         <  SRR19973066.1049728/150‑1 (MQ=60)
                                                                                                            GCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTG                                         >  SRR19973066.999472/1‑150 (MQ=60)
                                                                                                             CTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGC                                        >  SRR19973066.15955/1‑150 (MQ=60)
                                                                                                                TCGCCGCGCCACGGGAACGGGTGCAGACAGCGTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAAGTTTATGCGGATAAGACGGATGCCCCACAACCCAAATCAATTGGTCAAACGGGGTGTTGTTGACATAGTGCAGC                                     >  SRR19973066.1411031/1‑150 (MQ=60)
                                                                                                                 CGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCG                                    <  SRR19973066.1019150/150‑1 (MQ=60)
                                                                                                                 CGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCG                                    <  SRR19973066.1069934/150‑1 (MQ=60)
                                                                                                                 CGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCG                                    <  SRR19973066.2808831/150‑1 (MQ=60)
                                                                                                                 CGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGAGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCATTTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCG                                    <  SRR19973066.3478472/150‑1 (MQ=60)
                                                                                                                    CGCGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCA                                 <  SRR19973066.125821/150‑1 (MQ=60)
                                                                                                                      CGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACG                               <  SRR19973066.1368909/150‑1 (MQ=60)
                                                                                                                      CGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACG                               <  SRR19973066.1368936/150‑1 (MQ=60)
                                                                                                                      CGCCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACG                               <  SRR19973066.1368912/150‑1 (MQ=60)
                                                                                                                        CCACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGT                             >  SRR19973066.555104/1‑150 (MQ=60)
                                                                                                                         CACGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTC                            >  SRR19973066.2291513/1‑150 (MQ=60)
                                                                                                                           CGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAG                          <  SRR19973066.3512385/150‑1 (MQ=60)
                                                                                                                           CGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAG                          >  SRR19973066.2007656/1‑150 (MQ=60)
                                                                                                                           CGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAG                          >  SRR19973066.1355244/1‑150 (MQ=60)
                                                                                                                           CGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAG                          <  SRR19973066.1598591/150‑1 (MQ=60)
                                                                                                                           CGGGAACGGGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACAAAGTGCAGCGCCACGGTCAG                          >  SRR19973066.1973928/1‑150 (MQ=60)
                                                                                                                                   GGTGCAGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATGGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCG                  <  SRR19973066.232964/150‑1 (MQ=60)
                                                                                                                                        AGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCCAAAATTTAAGCGGATAAGCCTGATGCCCCAAATACCAAAACAAGTGGTCAAACGTGGTGTTGTAGACATAGTGCAGCGCCACGGACAGTTCGACCGTGCaa             >  SRR19973066.851619/1‑148 (MQ=60)
                                                                                                                                        AGACCGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCC             >  SRR19973066.31521/1‑150 (MQ=60)
                                                                                                                                            CGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCC         >  SRR19973066.3074138/1‑150 (MQ=60)
                                                                                                                                            CGCCTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCC         >  SRR19973066.3074144/1‑150 (MQ=60)
                                                                                                                                               CTTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGG      >  SRR19973066.2346503/1‑150 (MQ=60)
                                                                                                                                                TTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA     >  SRR19973066.3229892/1‑150 (MQ=60)
                                                                                                                                                TTTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGA     >  SRR19973066.3229894/1‑150 (MQ=60)
                                                                                                                                                 TTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAG    <  SRR19973066.869943/150‑1 (MQ=60)
                                                                                                                                                 TTCTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAG    <  SRR19973066.869949/150‑1 (MQ=60)
                                                                                                                                                   CTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGGC  >  SRR19973066.3091156/1‑150 (MQ=60)
                                                                                                                                                   CTTACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGGC  >  SRR19973066.876466/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATACGTCATATTCGCTTTCGCCGCGCCACGGGAACGGGTGCAGACCGCCTTTCTGACGGATGGTGCCGATTTTGTCGCGGCGTCCGGTCAAAATTTTATGCGGATAAGCCTGATGCCCCACATCCCAAATCAATTGGTCAAACGGGGTGTTGTAGACATAGTGCAGCGCCACGGTCAGTTCGACCGTGCCCAGCCCGGAGGC  >  CP009273/435187‑435483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑