Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 607,931 G→A 58.1% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273607,9310GA58.1% 141.4 / inf 260S7F (TCC→TTC) fepAiron‑enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (56/53);  new base A (85/66);  total (141/119)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 270 total aligned reads displayed.

CTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607785‑608077
                                                                                                                                                  |                                                                                                                                                  
 tGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaat                                                                                                                                                 >  2:3345397/1‑149 (MQ=255)
       gATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCAGAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAAtct                                                                                                                                           <  2:716424/149‑1 (MQ=255)
            tCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTg                                                                                                                                      >  1:779517/1‑149 (MQ=255)
            tCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTg                                                                                                                                      <  1:2060547/149‑1 (MQ=255)
            tCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTg                                                                                                                                      >  2:1735314/1‑149 (MQ=255)
               aaaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTc                                                                                                                                   >  1:627956/1‑149 (MQ=255)
               aaaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTc                                                                                                                                   >  1:1280002/1‑149 (MQ=255)
                 aCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAt                                                                                                                                 >  1:4952444/1‑149 (MQ=255)
                     cAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGACATGAATCTTCTTGTTCATTGtt                                                                                                                             >  2:209696/1‑149 (MQ=255)
                     cAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtt                                                                                                                             <  1:5168654/149‑1 (MQ=255)
                      aGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGttt                                                                                                                            <  2:2017034/149‑1 (MQ=255)
                       ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  2:2762014/1‑149 (MQ=255)
                       ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           <  2:4399444/149‑1 (MQ=255)
                       ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  1:1960604/1‑149 (MQ=255)
                       ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  1:5000449/1‑149 (MQ=255)
                       ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  2:2216300/1‑149 (MQ=255)
                          gcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTAtt                                                                                                                        <  1:1497320/149‑1 (MQ=255)
                          gcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTAtt                                                                                                                        >  1:3011070/1‑149 (MQ=255)
                           ccTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                       <  1:1051253/149‑1 (MQ=255)
                            cTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTcc                                                                                                                      <  2:1599511/149‑1 (MQ=255)
                            cTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTcc                                                                                                                      <  2:3552949/149‑1 (MQ=255)
                             tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCt                                                                                                                     <  2:4943456/149‑1 (MQ=255)
                             tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCt                                                                                                                     <  1:4349763/149‑1 (MQ=255)
                                  gTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAtt                                                                                                                <  2:2573181/149‑1 (MQ=255)
                                   ttCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAttt                                                                                                               >  1:3421242/1‑149 (MQ=255)
                                   ttCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAttt                                                                                                               <  1:1996773/149‑1 (MQ=255)
                                   ttCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAgtt                                                                                                               >  2:2801213/1‑149 (MQ=255)
                                     cTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTTCaaagaa                                                                                                             >  2:2654704/1‑144 (MQ=255)
                                       gCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGACACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGc                                                                                                           <  2:3307577/149‑1 (MQ=255)
                                         tCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                         >  1:3105699/1‑149 (MQ=255)
                                           ggcggcggTAACGAGAATAGTATCGTGCTTGGTATCAGGTGTATGGGTCGGCTCTTGTGCCTGCGCTACCGGATAAATCCCCTGATTGTGCAAGTTGGCGAGGATATGTTTCTTCTTGTTAATTGTTTTATTACTGAATTTTTGTCagg                                                                                                       <  2:687115/149‑3 (MQ=255)
                                              ggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAt                                                                                                    >  1:3883522/1‑149 (MQ=255)
                                              ggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAt                                                                                                    >  1:3770732/1‑149 (MQ=255)
                                               gcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAtt                                                                                                   <  2:3864666/149‑1 (MQ=255)
                                                  gTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCa                                                                                                >  1:101430/1‑149 (MQ=255)
                                                     aCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACt                                                                                             >  2:1696180/1‑149 (MQ=255)
                                                     aCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACt                                                                                             >  1:5113054/1‑149 (MQ=255)
                                                       gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGACTTGCAACTGt                                                                                           >  1:3831640/1‑149 (MQ=255)
                                                       gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGt                                                                                           >  1:733949/1‑149 (MQ=255)
                                                        aCAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTc                                                                                          <  2:2712745/149‑1 (MQ=255)
                                                           aTAGTATCGTCATGTGAAACAGGAGTAACGGTCGGCTCTTGTGCCTGCGCTACCCCAAAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTTTCggg                                                                                       >  2:4651333/1‑149 (MQ=255)
                                                            tAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGc                                                                                      >  1:2656421/1‑149 (MQ=255)
                                                             aGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCa                                                                                     >  2:3435840/1‑149 (MQ=255)
                                                                 tCGACATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgt                                                                                 <  1:4168862/149‑1 (MQ=255)
                                                                  cGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtc                                                                                <  1:3640222/149‑1 (MQ=255)
                                                                   gTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcg                                                                               >  2:1517582/1‑149 (MQ=255)
                                                                   gTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcg                                                                               >  1:2914138/1‑149 (MQ=255)
                                                                    tCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgt                                                                              >  1:1145961/1‑149 (MQ=255)
                                                                    tCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgt                                                                              >  1:1734990/1‑149 (MQ=255)
                                                                      aTGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtca                                                                            <  1:2202912/149‑1 (MQ=255)
                                                                      aTGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtca                                                                            >  2:612158/1‑149 (MQ=255)
                                                                          gAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCaa                                                                        <  2:5150844/149‑1 (MQ=255)
                                                                             aCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAacac                                                                     <  1:1244663/149‑1 (MQ=255)
                                                                              cAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCCTGGTCGTCATCAACacg                                                                    >  2:2497778/1‑149 (MQ=255)
                                                                              cAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacg                                                                    >  2:1612267/1‑149 (MQ=255)
                                                                               aGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacga                                                                   <  2:4982771/149‑1 (MQ=255)
                                                                               aGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacga                                                                   <  1:2558474/149‑1 (MQ=255)
                                                                                ggAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgac                                                                  <  1:4810574/149‑1 (MQ=255)
                                                                                ggAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgac                                                                  >  2:1678348/1‑149 (MQ=255)
                                                                                     aTCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATc                                                             <  1:3198969/149‑1 (MQ=255)
                                                                                     aTCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATc                                                             >  2:4677162/1‑149 (MQ=255)
                                                                                      tcggtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATcc                                                            >  2:2617865/1‑149 (MQ=255)
                                                                                      tcggtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATcc                                                            <  1:4098779/149‑1 (MQ=255)
                                                                                         gtcggCTCTTGTGCCTGCGCTACCCCATAACTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGc                                                         <  2:1017971/149‑1 (MQ=255)
                                                                                           cggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTa                                                       >  2:2917340/1‑149 (MQ=255)
                                                                                            ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                      >  2:2374845/1‑149 (MQ=255)
                                                                                            ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                      >  2:1208842/1‑149 (MQ=255)
                                                                                            ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                      <  2:2879772/149‑1 (MQ=255)
                                                                                             gCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACACGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGACACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                     >  1:558625/1‑149 (MQ=255)
                                                                                               tctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACcgc                                                   >  2:4773921/1‑149 (MQ=255)
                                                                                               tctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACcgc                                                   >  2:399245/1‑149 (MQ=255)
                                                                                                 ttGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGa                                                 >  1:2454535/1‑149 (MQ=255)
                                                                                                   gtgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCAGGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaa                                               >  2:80186/1‑149 (MQ=255)
                                                                                                    tgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaaa                                              >  2:2046139/1‑149 (MQ=255)
                                                                                                    tgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaaa                                              <  2:5123855/149‑1 (MQ=255)
                                                                                                         gcgcTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCttt                                         <  1:1587522/149‑1 (MQ=255)
                                                                                                           gcTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGa                                       <  2:3011070/149‑1 (MQ=255)
                                                                                                              aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATcc                                    >  2:3239512/1‑149 (MQ=255)
                                                                                                              aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATcc                                    >  1:1668021/1‑149 (MQ=255)
                                                                                                              aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATcc                                    <  1:509531/149‑1 (MQ=255)
                                                                                                              aCCACATAAATGCCAAGATTGACCAAATAGGACTGGAAATTAATCTTCTTGTTATTTGTTTTATTCCTGCTTTTTTGCCACGAATTGCAACTGTAGGTCATGGTCGTCATCAAGACGACGCATCCCTCTACCGCGATAACATTTTAtaa                                    <  2:754716/149‑3 (MQ=255)
                                                                                                                   aTAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGaaa                               <  2:2238919/149‑1 (MQ=255)
                                                                                                                   aTAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGaaa                               <  1:4270965/149‑1 (MQ=255)
                                                                                                                     aaaTCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  2:4732676/149‑1 (MQ=255)
                                                                                                                        tCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          >  1:3539027/1‑149 (MQ=255)
                                                                                                                        tCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          <  1:2762014/149‑1 (MQ=255)
                                                                                                                        tCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          >  1:3372532/1‑149 (MQ=255)
                                                                                                                        tCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          <  2:4262449/149‑1 (MQ=255)
                                                                                                                          cccAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgc                        <  2:2137465/149‑1 (MQ=255)
                                                                                                                            cAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcag                      <  1:874527/149‑1 (MQ=255)
                                                                                                                             agagTGACCAACAAGGCCAGGAACTGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagt                     <  2:3897576/149‑1 (MQ=255)
                                                                                                                              gATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagtg                    >  1:535168/1‑149 (MQ=255)
                                                                                                                               aTTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagtgc                   <  1:853359/149‑1 (MQ=255)
                                                                                                                                  gACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagtgcagt                <  2:506130/149‑1 (MQ=255)
                                                                                                                                     caacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTgg             >  1:1851053/1‑149 (MQ=255)
                                                                                                                                      aacaaGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGt            >  2:4191833/1‑149 (MQ=255)
                                                                                                                                      aacaaGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGt            >  1:2601593/1‑149 (MQ=255)
                                                                                                                                        caaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtta          >  2:3087142/1‑149 (MQ=255)
                                                                                                                                        caaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGtta          >  2:3086199/1‑149 (MQ=255)
                                                                                                                                         aaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaa         >  1:3123595/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607785‑608077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCC  >  CP009273/607782‑608079
                                                                                                                                                     |                                                                                                                                                    
CATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGG                                                                                                                                                      <  SRR19973057.697354/150‑1 (MQ=60)
       GGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAT                                                                                                                                               <  SRR19973057.685162/150‑1 (MQ=60)
         TGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCT                                                                                                                                             >  SRR19973057.3835114/1‑150 (MQ=60)
            TGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCT                                                                                                                                          <  SRR19973057.4858185/150‑1 (MQ=60)
            TGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCT                                                                                                                                          <  SRR19973057.2493748/150‑1 (MQ=60)
              GTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTG                                                                                                                                        >  SRR19973057.782488/1‑150 (MQ=60)
              GTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTG                                                                                                                                        >  SRR19973057.782592/1‑150 (MQ=60)
               TCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGT                                                                                                                                       <  SRR19973057.2068894/150‑1 (MQ=60)
               TCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGT                                                                                                                                       <  SRR19973057.2554772/150‑1 (MQ=60)
               TCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGT                                                                                                                                       <  SRR19973057.5166274/150‑1 (MQ=60)
                 GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGTGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTC                                                                                                                                     >  SRR19973057.2677631/1‑150 (MQ=60)
                 GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCTGCGGTAACGAAAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTTTGCCTGCGCTACCCCATAAATACCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTC                                                                                                                                     >  SRR19973057.63155/1‑150 (MQ=60)
                 GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTC                                                                                                                                     >  SRR19973057.630570/1‑150 (MQ=60)
                 GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTC                                                                                                                                     >  SRR19973057.1284903/1‑150 (MQ=60)
                  AAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCA                                                                                                                                    >  SRR19973057.4166009/1‑150 (MQ=60)
                  AAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCA                                                                                                                                    >  SRR19973057.4166514/1‑150 (MQ=60)
                   AACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCAT                                                                                                                                   <  SRR19973057.339432/150‑1 (MQ=60)
                   AACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCAT                                                                                                                                   <  SRR19973057.2020594/150‑1 (MQ=60)
                   AACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAT                                                                                                                                   >  SRR19973057.4972043/1‑150 (MQ=60)
                   AACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAT                                                                                                                                   >  SRR19973057.1639662/1‑150 (MQ=60)
                   AACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAT                                                                                                                                   >  SRR19973057.2084939/1‑150 (MQ=60)
                     CGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTG                                                                                                                                 <  SRR19973057.2668607/150‑1 (MQ=60)
                       CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTT                                                                                                                               <  SRR19973057.4054259/150‑1 (MQ=60)
                        CAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTT                                                                                                                              <  SRR19973057.5188920/150‑1 (MQ=60)
                        CAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGATCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTT                                                                                                                              <  SRR19973057.5189146/150‑1 (MQ=60)
                         AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                             >  SRR19973057.3875566/1‑150 (MQ=60)
                         AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                             >  SRR19973057.5020250/1‑150 (MQ=60)
                         AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                             >  SRR19973057.5020100/1‑150 (MQ=60)
                         AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                             >  SRR19973057.1968826/1‑150 (MQ=60)
                         AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                             >  SRR19973057.1968629/1‑150 (MQ=60)
                          GGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTA                                                                                                                            <  SRR19973057.1571606/150‑1 (MQ=60)
                            CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                          <  SRR19973057.1089865/150‑1 (MQ=60)
                            CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                          >  SRR19973057.3022977/1‑150 (MQ=60)
                            CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                          <  SRR19973057.1871379/150‑1 (MQ=60)
                             GCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTC                                                                                                                         <  SRR19973057.1503410/150‑1 (MQ=60)
                              CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCATATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                        <  SRR19973057.3583642/150‑1 (MQ=60)
                              CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                        <  SRR19973057.1751343/150‑1 (MQ=60)
                              CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                        >  SRR19973057.3723318/1‑150 (MQ=60)
                              CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                        <  SRR19973057.1055523/150‑1 (MQ=60)
                                TGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTG                                                                                                                      <  SRR19973057.4366801/150‑1 (MQ=60)
                                TGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTG                                                                                                                      >  SRR19973057.1924268/1‑150 (MQ=60)
                                  TAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCA                                                                                                                    <  SRR19973057.817869/150‑1 (MQ=60)
                                  TAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCA                                                                                                                    <  SRR19973057.3428177/150‑1 (MQ=60)
                                   AAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAT                                                                                                                   >  SRR19973057.1348280/1‑150 (MQ=60)
                                   AAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAT                                                                                                                   >  SRR19973057.3684310/1‑150 (MQ=60)
                                    AGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATT                                                                                                                  <  SRR19973057.1729266/150‑1 (MQ=60)
                                     GTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTT                                                                                                                 >  SRR19973057.3434713/1‑150 (MQ=60)
                                      TTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTT                                                                                                                <  SRR19973057.1276906/150‑1 (MQ=60)
                                      TTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTT                                                                                                                <  SRR19973057.2004940/150‑1 (MQ=60)
                                           CTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCA                                                                                                           >  SRR19973057.3118140/1‑150 (MQ=60)
                                           CTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCA                                                                                                           >  SRR19973057.3118150/1‑150 (MQ=60)
                                                CGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAT                                                                                                      >  SRR19973057.3785678/1‑150 (MQ=60)
                                                CGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAT                                                                                                      >  SRR19973057.3898725/1‑150 (MQ=60)
                                                 GGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATT                                                                                                     >  SRR19973057.3320710/1‑150 (MQ=60)
                                                    GGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCA                                                                                                  >  SRR19973057.3870891/1‑150 (MQ=60)
                                                    GGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCA                                                                                                  >  SRR19973057.101984/1‑150 (MQ=60)
                                                      TAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAAC                                                                                                >  SRR19973057.2078321/1‑150 (MQ=60)
                                                       AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                               >  SRR19973057.5133188/1‑150 (MQ=60)
                                                       AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                               >  SRR19973057.4214366/1‑150 (MQ=60)
                                                       AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                               >  SRR19973057.5149802/1‑150 (MQ=60)
                                                         CGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGACTTGCAACTGT                                                                                             >  SRR19973057.3846728/1‑150 (MQ=60)
                                                         CGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGT                                                                                             >  SRR19973057.3846684/1‑150 (MQ=60)
                                                         CGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGT                                                                                             >  SRR19973057.736920/1‑150 (MQ=60)
                                                             AATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGG                                                                                         <  SRR19973057.1428603/150‑1 (MQ=60)
                                                              ATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGC                                                                                        >  SRR19973057.508164/1‑150 (MQ=60)
                                                              ATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGC                                                                                        >  SRR19973057.2667166/1‑150 (MQ=60)
                                                                AGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCAT                                                                                      >  SRR19973057.541601/1‑150 (MQ=60)
                                                                 GTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATG                                                                                     <  SRR19973057.4592933/150‑1 (MQ=60)
                                                                    TCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTC                                                                                  <  SRR19973057.3090381/150‑1 (MQ=60)
                                                                    TCGACATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTC                                                                                  <  SRR19973057.4185397/150‑1 (MQ=60)
                                                                     CGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCG                                                                                 <  SRR19973057.3654751/150‑1 (MQ=60)
                                                                     CGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCG                                                                                 >  SRR19973057.2925823/1‑150 (MQ=60)
                                                                      GTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGT                                                                                >  SRR19973057.1741789/1‑150 (MQ=60)
                                                                      GTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGT                                                                                >  SRR19973057.1150560/1‑150 (MQ=60)
                                                                       TCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTC                                                                               >  SRR19973057.430590/1‑150 (MQ=60)
                                                                        CATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCA                                                                              >  SRR19973057.1248539/1‑150 (MQ=60)
                                                                         ATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCAT                                                                             <  SRR19973057.2211745/150‑1 (MQ=60)
                                                                         ATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCAT                                                                             >  SRR19973057.2630389/1‑150 (MQ=60)
                                                                                ACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACG                                                                      <  SRR19973057.1249496/150‑1 (MQ=60)
                                                                                  AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGAC                                                                    <  SRR19973057.2569304/150‑1 (MQ=60)
                                                                                  AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGAC                                                                    <  SRR19973057.2568818/150‑1 (MQ=60)
                                                                                   GGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACG                                                                   <  SRR19973057.4829507/150‑1 (MQ=60)
                                                                                   GGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACG                                                                   <  SRR19973057.4829565/150‑1 (MQ=60)
                                                                                      GTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATACATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCAT                                                                <  SRR19973057.1033784/150‑1 (MQ=60)
                                                                                      GTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCAT                                                                >  SRR19973057.1238859/1‑150 (MQ=60)
                                                                                        ATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCC                                                              <  SRR19973057.3211823/150‑1 (MQ=60)
                                                                                         TCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCC                                                             <  SRR19973057.4115103/150‑1 (MQ=60)
                                                                                               GGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACACGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGACACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACC                                                       >  SRR19973057.560938/1‑150 (MQ=60)
                                                                                                  TCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCG                                                    >  SRR19973057.786265/1‑150 (MQ=60)
                                                                                                   CTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGA                                                   >  SRR19973057.2464587/1‑150 (MQ=60)
                                                                                                          CTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTT                                            >  SRR19973057.5125649/1‑150 (MQ=60)
                                                                                                            GCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTG                                          <  SRR19973057.1593254/150‑1 (MQ=60)
                                                                                                            GCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTG                                          <  SRR19973057.1593918/150‑1 (MQ=60)
                                                                                                                TACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCC                                      >  SRR19973057.584735/1‑150 (MQ=60)
                                                                                                                TACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCC                                      <  SRR19973057.2042840/150‑1 (MQ=60)
                                                                                                                TACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCC                                      >  SRR19973057.1674656/1‑150 (MQ=60)
                                                                                                                 ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                     <  SRR19973057.511579/150‑1 (MQ=60)
                                                                                                                 ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                     <  SRR19973057.511641/150‑1 (MQ=60)
                                                                                                                  CCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTG                                    >  SRR19973057.782152/1‑150 (MQ=60)
                                                                                                                      ATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAG                                <  SRR19973057.4287771/150‑1 (MQ=60)
                                                                                                                        AAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGAC                              <  SRR19973057.2869189/150‑1 (MQ=60)
                                                                                                                         AATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACA                             >  SRR19973057.1079527/1‑150 (MQ=60)
                                                                                                                          ATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                            >  SRR19973057.3553098/1‑150 (MQ=60)
                                                                                                                          ATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                            <  SRR19973057.3449352/150‑1 (MQ=60)
                                                                                                                          ATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                            <  SRR19973057.3449361/150‑1 (MQ=60)
                                                                                                                          ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                            >  SRR19973057.3385921/1‑150 (MQ=60)
                                                                                                                           TCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACG                           <  SRR19973057.2773211/150‑1 (MQ=60)
                                                                                                                               CAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGT                       >  SRR19973057.5060618/1‑150 (MQ=60)
                                                                                                                               CAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGT                       <  SRR19973057.877811/150‑1 (MQ=60)
                                                                                                                                AGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTG                      >  SRR19973057.537360/1‑150 (MQ=60)
                                                                                                                                AGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTG                      >  SRR19973057.537454/1‑150 (MQ=60)
                                                                                                                                 GATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGC                     >  SRR19973057.2596621/1‑150 (MQ=60)
                                                                                                                                 GATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGC                     >  SRR19973057.2966533/1‑150 (MQ=60)
                                                                                                                                 GATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGC                     >  SRR19973057.2966397/1‑150 (MQ=60)
                                                                                                                                  ATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCA                    <  SRR19973057.856630/150‑1 (MQ=60)
                                                                                                                                  ATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCA                    <  SRR19973057.856579/150‑1 (MQ=60)
                                                                                                                                  ATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCA                    >  SRR19973057.180741/1‑150 (MQ=60)
                                                                                                                                       CCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGG               <  SRR19973057.4652974/150‑1 (MQ=60)
                                                                                                                                       CCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGG               >  SRR19973057.1858571/1‑150 (MQ=60)
                                                                                                                                        CAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGT              >  SRR19973057.2612024/1‑150 (MQ=60)
                                                                                                                                         AACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTT             <  SRR19973057.2853717/150‑1 (MQ=60)
                                                                                                                                           CAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAA           <  SRR19973057.169154/150‑1 (MQ=60)
                                                                                                                                           CAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAA           >  SRR19973057.3136037/1‑150 (MQ=60)
                                                                                                                                           CAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAA           >  SRR19973057.3136117/1‑150 (MQ=60)
                                                                                                                                           CAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAA           >  SRR19973057.3136290/1‑150 (MQ=60)
                                                                                                                                             AGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATT         <  SRR19973057.3142591/150‑1 (MQ=60)
                                                                                                                                             AGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATT         >  SRR19973057.905919/1‑150 (MQ=60)
                                                                                                                                                 CAGGAAATGAATCGTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATG     <  SRR19973057.2225219/150‑1 (MQ=60)
                                                                                                                                                  AGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT    <  SRR19973057.4219679/150‑1 (MQ=60)
                                                                                                                                                  AGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT    >  SRR19973057.1952748/1‑150 (MQ=60)
                                                                                                                                                  AGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT    >  SRR19973057.1573874/1‑150 (MQ=60)
                                                                                                                                                    GAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCC  <  SRR19973057.3706210/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCC  >  CP009273/607782‑608079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑