Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 607,931 G→A 100% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273607,9310GA100.0% 798.3 / ‑6.7 238S7F (TCC→TTC) fepAiron‑enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (127/109);  minor base C (0/1);  total (127/111)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

TCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607784‑608077
                                                                                                                                                   |                                                                                                                                                  
tCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaa                                                                                                                                                  >  2:1112685/1‑150 (MQ=255)
  tGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaatg                                                                                                                                                <  2:2419998/150‑1 (MQ=255)
   gCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTATTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaatga                                                                                                                                               <  2:2597242/150‑1 (MQ=255)
        gATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtctt                                                                                                                                          >  1:3509717/1‑150 (MQ=255)
        gATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtctt                                                                                                                                          <  2:1146999/150‑1 (MQ=255)
          tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGTCCAGGAAATGAAtcttct                                                                                                                                        >  1:575996/1‑150 (MQ=255)
          tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttct                                                                                                                                        <  1:645202/150‑1 (MQ=255)
             tCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGt                                                                                                                                     >  1:4327903/1‑150 (MQ=255)
               gAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTc                                                                                                                                   >  2:3329603/1‑150 (MQ=255)
               gAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTc                                                                                                                                   >  2:2046570/1‑150 (MQ=255)
               gAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTc                                                                                                                                   >  1:761778/1‑150 (MQ=255)
               gAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAACTTCTTGTTc                                                                                                                                   <  1:1987313/150‑1 (MQ=255)
               gAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCAGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTc                                                                                                                                   >  2:744964/1‑150 (MQ=255)
                aaaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCa                                                                                                                                  >  2:4247115/1‑150 (MQ=255)
                   cGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTg                                                                                                                               >  2:683846/1‑150 (MQ=255)
                    gCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATACATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGt                                                                                                                              <  2:516211/150‑1 (MQ=255)
                    gCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGt                                                                                                                              <  2:3550552/150‑1 (MQ=255)
                     ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtt                                                                                                                             <  1:4712877/150‑1 (MQ=255)
                       aGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                           >  2:3187818/1‑150 (MQ=255)
                        ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTa                                                                                                                          <  2:1119690/150‑1 (MQ=255)
                          cgcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTAtt                                                                                                                        <  1:4519916/150‑1 (MQ=255)
                           gcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                       <  1:2151413/150‑1 (MQ=255)
                           gcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                       <  2:1594517/150‑1 (MQ=255)
                               gTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGc                                                                                                                   >  1:2542511/1‑150 (MQ=255)
                                  aGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAtt                                                                                                                <  1:1614077/150‑1 (MQ=255)
                                    ttCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAtttt                                                                                                              <  2:2899411/150‑1 (MQ=255)
                                      cTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTg                                                                                                            >  1:596308/1‑150 (MQ=255)
                                        gCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGcc                                                                                                          <  2:2229781/150‑1 (MQ=255)
                                         cTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                         <  1:2264669/150‑1 (MQ=255)
                                           cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACg                                                                                                       >  2:296572/1‑150 (MQ=255)
                                           cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACg                                                                                                       >  2:5140057/1‑150 (MQ=255)
                                           cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACg                                                                                                       <  2:161149/150‑1 (MQ=255)
                                             gcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGaa                                                                                                     >  1:1089372/1‑150 (MQ=255)
                                             gcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGaa                                                                                                     >  1:1617211/1‑150 (MQ=255)
                                              cggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAt                                                                                                    <  2:4443848/150‑1 (MQ=255)
                                               ggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAtt                                                                                                   <  1:940993/150‑1 (MQ=255)
                                                  ggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCa                                                                                                <  2:3839608/150‑1 (MQ=255)
                                                   gTAACGACAATAGTATCGTCATGTGAAACAGGAGTANCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                                                               >  1:2679699/1‑150 (MQ=255)
                                                    tAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAAc                                                                                              <  2:3416570/150‑1 (MQ=255)
                                                     aaCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGACTCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACt                                                                                             >  2:4398901/1‑150 (MQ=255)
                                                     aaCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACt                                                                                             >  2:2095056/1‑150 (MQ=255)
                                                     aaCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTATTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTTTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACt                                                                                             <  1:809574/150‑1 (MQ=255)
                                                        gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTc                                                                                          <  2:2800114/150‑1 (MQ=255)
                                                        gACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTc                                                                                          <  2:2273257/150‑1 (MQ=255)
                                                            aTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGc                                                                                      >  2:4929718/1‑150 (MQ=255)
                                                             tAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCa                                                                                     >  1:3652490/1‑150 (MQ=255)
                                                                 aTCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgt                                                                                 <  2:498118/150‑1 (MQ=255)
                                                                 aTCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgt                                                                                 >  2:4111547/1‑150 (MQ=255)
                                                                         gtgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtcatca                                                                         >  2:3281726/1‑150 (MQ=255)
                                                                          tgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCaa                                                                        >  2:477539/1‑150 (MQ=255)
                                                                          tgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCaa                                                                        >  1:3952218/1‑150 (MQ=255)
                                                                             aaCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAacac                                                                     <  1:1504949/150‑1 (MQ=255)
                                                                             aaCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAacac                                                                     >  1:1352955/1‑150 (MQ=255)
                                                                              aCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacg                                                                    >  2:3518798/1‑150 (MQ=255)
                                                                               cAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacga                                                                   >  1:3322221/1‑150 (MQ=255)
                                                                                aGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgac                                                                  >  1:1879353/1‑150 (MQ=255)
                                                                                 ggAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgacg                                                                 >  1:2485770/1‑150 (MQ=255)
                                                                                 ggAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgacg                                                                 <  2:1823921/150‑1 (MQ=255)
                                                                                          gtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCt                                                        <  2:3405112/150‑1 (MQ=255)
                                                                                          gtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCt                                                        >  2:207185/1‑150 (MQ=255)
                                                                                           tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTa                                                       <  1:1634912/150‑1 (MQ=255)
                                                                                           tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTa                                                       <  1:1458169/150‑1 (MQ=255)
                                                                                           tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTa                                                       <  1:3001301/150‑1 (MQ=255)
                                                                                            cggCTCTTGTGCCTGCGCTACCCCATAACTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                      <  2:36483/150‑1 (MQ=255)
                                                                                            cggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                      <  1:2983679/150‑1 (MQ=255)
                                                                                            cggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTAATGCATTTTTTCAACGAATTGCAAATGTCGGGCATGGTCGTCATCAACACGAAGCATCCCTCTAc                                                      >  2:1439952/1‑150 (MQ=255)
                                                                                             ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                     <  2:629025/150‑1 (MQ=255)
                                                                                             ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                     >  2:1912754/1‑150 (MQ=255)
                                                                                             ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                     >  2:4874567/1‑150 (MQ=255)
                                                                                             ggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTAGTTCATTGTTTTATTACTGAATTTTTGCCACGAATTGCAACAGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                     >  2:3932990/1‑150 (MQ=255)
                                                                                              gCTCTTGGGCCTGCGCGACCCCAGACCTCCCCAGATGGACCCACAAGGCCAGGAAATGAATCTTCTGGTTCATTGTTTTAGTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACcg                                                    <  2:875982/150‑1 (MQ=255)
                                                                                                 ctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGa                                                 >  1:3234305/1‑150 (MQ=255)
                                                                                                  ttGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaa                                                >  1:1385335/1‑150 (MQ=255)
                                                                                                   tgtgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaa                                               <  2:102919/150‑1 (MQ=255)
                                                                                                     tgCCTGCGCTACCCCATACATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAc                                             <  1:4757722/150‑1 (MQ=255)
                                                                                                     tgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAc                                             <  1:1878758/150‑1 (MQ=255)
                                                                                                     tgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAc                                             >  2:2528205/1‑150 (MQ=255)
                                                                                                        cTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGTAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCtt                                          <  2:4283086/150‑1 (MQ=255)
                                                                                                        cTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCtt                                          <  2:1801834/150‑1 (MQ=255)
                                                                                                         tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCttt                                         <  1:3539428/150‑1 (MQ=255)
                                                                                                             cTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATc                                     <  1:5045495/150‑1 (MQ=255)
                                                                                                               aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCt                                   <  1:4513841/150‑1 (MQ=255)
                                                                                                               aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCt                                   >  1:1972284/1‑150 (MQ=255)
                                                                                                               aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCt                                   >  2:1293997/1‑150 (MQ=255)
                                                                                                               aCCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCt                                   >  1:4846454/1‑150 (MQ=255)
                                                                                                                  ccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGaa                                <  1:3829714/150‑1 (MQ=255)
                                                                                                                     tAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGa                             <  2:2648432/150‑1 (MQ=255)
                                                                                                                       aaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGaca                           >  1:3232783/1‑150 (MQ=255)
                                                                                                                        aTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          <  2:301912/150‑1 (MQ=255)
                                                                                                                        aTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                          >  2:1992511/1‑150 (MQ=255)
                                                                                                                              aGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagtg                    >  1:1645478/1‑150 (MQ=255)
                                                                                                                               gATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgcagtgc                   >  1:3289523/1‑150 (MQ=255)
                                                                                                                                    aCCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTg              >  2:1360110/1‑150 (MQ=255)
                                                                                                                                            ggCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaatta      <  2:2327193/150‑1 (MQ=255)
                                                                                                                                            ggCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaatta      >  1:2749172/1‑150 (MQ=255)
                                                                                                                                             gCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaattaa     >  2:3075493/1‑150 (MQ=255)
                                                                                                                                              ccAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTtaattaat    >  2:3219936/1‑150 (MQ=255)
                                                                                                                                               cAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATg   <  1:4155701/150‑1 (MQ=255)
                                                                                                                                                aGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGt  <  1:3776060/150‑1 (MQ=255)
                                                                                                                                                aGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGt  <  1:4247115/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT  >  CP009273/607784‑608077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTC  >  CP009273/607786‑608078
                                                                                                                                                 |                                                                                                                                                   
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATG                                                                                                                                                 >  SRR19973063.912441/1‑150 (MQ=60)
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATG                                                                                                                                                 <  SRR19973063.2887954/150‑1 (MQ=60)
      GATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTT                                                                                                                                           >  SRR19973063.3520940/1‑150 (MQ=60)
        TGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGTCCAGGAAATGAATCTTCT                                                                                                                                         >  SRR19973063.577691/1‑150 (MQ=60)
        TGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCT                                                                                                                                         <  SRR19973063.647094/150‑1 (MQ=60)
        TGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCT                                                                                                                                         <  SRR19973063.579805/150‑1 (MQ=60)
         GGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTT                                                                                                                                        >  SRR19973063.3892022/1‑150 (MQ=60)
           TCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGT                                                                                                                                      >  SRR19973063.4341693/1‑150 (MQ=60)
             GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTC                                                                                                                                    <  SRR19973063.2538255/150‑1 (MQ=60)
             GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTC                                                                                                                                    >  SRR19973063.3246391/1‑150 (MQ=60)
             GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTC                                                                                                                                    >  SRR19973063.763999/1‑150 (MQ=60)
             GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAACTTCTTGTTC                                                                                                                                    <  SRR19973063.1993525/150‑1 (MQ=60)
                 CGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTG                                                                                                                                >  SRR19973063.2334644/1‑150 (MQ=60)
                  GCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGT                                                                                                                               >  SRR19973063.2749698/1‑150 (MQ=60)
                   CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTT                                                                                                                              <  SRR19973063.3103234/150‑1 (MQ=60)
                   CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTT                                                                                                                              <  SRR19973063.4727910/150‑1 (MQ=60)
                    CAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTT                                                                                                                             >  SRR19973063.1488888/1‑150 (MQ=60)
                     AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                            >  SRR19973063.15642/1‑150 (MQ=60)
                       GCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTAT                                                                                                                          <  SRR19973063.3839173/150‑1 (MQ=60)
                        CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                         <  SRR19973063.3270654/150‑1 (MQ=60)
                        CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                         <  SRR19973063.4494517/150‑1 (MQ=60)
                        CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                         <  SRR19973063.4534419/150‑1 (MQ=60)
                         GCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTC                                                                                                                        <  SRR19973063.2158151/150‑1 (MQ=60)
                           CTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATACATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCT                                                                                                                      <  SRR19973063.4242191/150‑1 (MQ=60)
                            TGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTG                                                                                                                     <  SRR19973063.1590843/150‑1 (MQ=60)
                             GTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGC                                                                                                                    >  SRR19973063.2550616/1‑150 (MQ=60)
                              TAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCA                                                                                                                   >  SRR19973063.144801/1‑150 (MQ=60)
                              TAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCA                                                                                                                   >  SRR19973063.284445/1‑150 (MQ=60)
                                AGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATT                                                                                                                 <  SRR19973063.4205345/150‑1 (MQ=60)
                                AGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATT                                                                                                                 <  SRR19973063.1619015/150‑1 (MQ=60)
                                    CTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTG                                                                                                             >  SRR19973063.598063/1‑150 (MQ=60)
                                     TGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGC                                                                                                            >  SRR19973063.3044409/1‑150 (MQ=60)
                                       CTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCA                                                                                                          <  SRR19973063.5081504/150‑1 (MQ=60)
                                       CTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCA                                                                                                          <  SRR19973063.2271884/150‑1 (MQ=60)
                                        TCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCAC                                                                                                         >  SRR19973063.3420903/1‑150 (MQ=60)
                                        TCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCAC                                                                                                         >  SRR19973063.3420907/1‑150 (MQ=60)
                                           GCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAA                                                                                                      >  SRR19973063.1622157/1‑150 (MQ=60)
                                           GCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAA                                                                                                      >  SRR19973063.1622111/1‑150 (MQ=60)
                                           GCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAA                                                                                                      >  SRR19973063.1092683/1‑150 (MQ=60)
                                             GGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATT                                                                                                    <  SRR19973063.943800/150‑1 (MQ=60)
                                             GGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAACTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATT                                                                                                    <  SRR19973063.4038395/150‑1 (MQ=60)
                                              GCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTG                                                                                                   <  SRR19973063.2591977/150‑1 (MQ=60)
                                               CGGTAACGACAATAGTATCGTCATGTGAACCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGCTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGC                                                                                                  <  SRR19973063.2678979/150‑1 (MQ=60)
                                                 GTAACGACAATAGTATCGTCATGTGAAACAGGAGTANCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                >  SRR19973063.2688327/1‑150 (MQ=60)
                                                  TAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAAC                                                                                               >  SRR19973063.197899/1‑150 (MQ=60)
                                                   AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                              <  SRR19973063.798018/150‑1 (MQ=60)
                                                   AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTATTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTTTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                              <  SRR19973063.811904/150‑1 (MQ=60)
                                                      GACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTC                                                                                           <  SRR19973063.1649075/150‑1 (MQ=60)
                                                          ATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGC                                                                                       >  SRR19973063.2343927/1‑150 (MQ=60)
                                                           TAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCA                                                                                      >  SRR19973063.3664202/1‑150 (MQ=60)
                                                             GTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATG                                                                                    <  SRR19973063.4793234/150‑1 (MQ=60)
                                                               ATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGT                                                                                  <  SRR19973063.2069738/150‑1 (MQ=60)
                                                               ATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGT                                                                                  <  SRR19973063.2069652/150‑1 (MQ=60)
                                                                TCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTC                                                                                 <  SRR19973063.1635266/150‑1 (MQ=60)
                                                                 CGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCG                                                                                >  SRR19973063.47842/1‑150 (MQ=60)
                                                                   TCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTC                                                                              >  SRR19973063.3325115/1‑150 (MQ=60)
                                                                    CATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGACTTGCAACTGTCGGGCATGGTCGTCA                                                                             >  SRR19973063.3673935/1‑150 (MQ=60)
                                                                    CATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCA                                                                             >  SRR19973063.3673811/1‑150 (MQ=60)
                                                                        TGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAA                                                                         >  SRR19973063.3964857/1‑150 (MQ=60)
                                                                        TGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAA                                                                         >  SRR19973063.3964732/1‑150 (MQ=60)
                                                                          AAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACA                                                                       >  SRR19973063.2232223/1‑150 (MQ=60)
                                                                           AACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACAC                                                                      <  SRR19973063.1509558/150‑1 (MQ=60)
                                                                           AACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACAC                                                                      >  SRR19973063.1357017/1‑150 (MQ=60)
                                                                             CAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGA                                                                    >  SRR19973063.3332831/1‑150 (MQ=60)
                                                                              AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGAC                                                                   >  SRR19973063.1885225/1‑150 (MQ=60)
                                                                              AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGAC                                                                   >  SRR19973063.1885226/1‑150 (MQ=60)
                                                                              AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGAC                                                                   >  SRR19973063.1885234/1‑150 (MQ=60)
                                                                               GGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACG                                                                  >  SRR19973063.2493714/1‑150 (MQ=60)
                                                                                     TCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCC                                                            <  SRR19973063.3600919/150‑1 (MQ=60)
                                                                                         TCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTA                                                        <  SRR19973063.1462615/150‑1 (MQ=60)
                                                                                         TCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTA                                                        >  SRR19973063.4594587/1‑150 (MQ=60)
                                                                                         TCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTA                                                        <  SRR19973063.1639910/150‑1 (MQ=60)
                                                                                         TCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTA                                                        <  SRR19973063.3010874/150‑1 (MQ=60)
                                                                                          CGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAC                                                       <  SRR19973063.2993241/150‑1 (MQ=60)
                                                                                          CGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAC                                                       <  SRR19973063.2993208/150‑1 (MQ=60)
                                                                                           GGCTCTTGTGCCTGCGCTACCCCATAACTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACC                                                      <  SRR19973063.454250/150‑1 (MQ=60)
                                                                                           GGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACC                                                      <  SRR19973063.1888297/150‑1 (MQ=60)
                                                                                            GCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCG                                                     <  SRR19973063.772211/150‑1 (MQ=60)
                                                                                               CTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGA                                                  >  SRR19973063.3244697/1‑150 (MQ=60)
                                                                                               CTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGA                                                  >  SRR19973063.3244646/1‑150 (MQ=60)
                                                                                                TTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAA                                                 >  SRR19973063.1389620/1‑150 (MQ=60)
                                                                                                TTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAA                                                 >  SRR19973063.1389524/1‑150 (MQ=60)
                                                                                                TTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAA                                                 >  SRR19973063.1827512/1‑150 (MQ=60)
                                                                                                   TGCCTGCGCTACCCCATACATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAC                                              <  SRR19973063.4772894/150‑1 (MQ=60)
                                                                                                   TGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAAC                                              <  SRR19973063.1884628/150‑1 (MQ=60)
                                                                                                       TGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTT                                          <  SRR19973063.3550768/150‑1 (MQ=60)
                                                                                                         CGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGA                                        <  SRR19973063.3530056/150‑1 (MQ=60)
                                                                                                          GCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAT                                       >  SRR19973063.1329211/1‑150 (MQ=60)
                                                                                                          GCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAT                                       <  SRR19973063.4519480/150‑1 (MQ=60)
                                                                                                           CTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATC                                      <  SRR19973063.5061749/150‑1 (MQ=60)
                                                                                                             ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                    <  SRR19973063.4528333/150‑1 (MQ=60)
                                                                                                             ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                    >  SRR19973063.1978452/1‑150 (MQ=60)
                                                                                                             ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                    <  SRR19973063.4528342/150‑1 (MQ=60)
                                                                                                             ACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCT                                    >  SRR19973063.4862050/1‑150 (MQ=60)
                                                                                                              CCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTG                                   >  SRR19973063.169607/1‑150 (MQ=60)
                                                                                                                CCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAA                                 <  SRR19973063.3841897/150‑1 (MQ=60)
                                                                                                                 CATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAA                                <  SRR19973063.53661/150‑1 (MQ=60)
                                                                                                                     AATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACA                            >  SRR19973063.3243171/1‑150 (MQ=60)
                                                                                                                        CCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGC                         >  SRR19973063.756851/1‑150 (MQ=60)
                                                                                                                           CAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGT                      >  SRR19973063.613117/1‑150 (MQ=60)
                                                                                                                            AGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTG                     >  SRR19973063.4949908/1‑150 (MQ=60)
                                                                                                                            AGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTG                     >  SRR19973063.1650506/1‑150 (MQ=60)
                                                                                                                             GATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGC                    >  SRR19973063.1629761/1‑150 (MQ=60)
                                                                                                                             GATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGC                    >  SRR19973063.3300063/1‑150 (MQ=60)
                                                                                                                              ATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCA                   >  SRR19973063.4300550/1‑150 (MQ=60)
                                                                                                                              ATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCA                   >  SRR19973063.4739377/1‑150 (MQ=60)
                                                                                                                                  ACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTG               >  SRR19973063.1386538/1‑150 (MQ=60)
                                                                                                                                         AGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATACTGAAAGACACGCAGTGCAGTTGGTTAATT        >  SRR19973063.62619/1‑150 (MQ=60)
                                                                                                                                          GGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTA       >  SRR19973063.2758040/1‑150 (MQ=60)
                                                                                                                                             CAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATG    <  SRR19973063.4168991/150‑1 (MQ=60)
                                                                                                                                              AGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT   <  SRR19973063.3788118/150‑1 (MQ=60)
                                                                                                                                              AGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGT   <  SRR19973063.4260649/150‑1 (MQ=60)
                                                                                                                                               GGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTC  >  SRR19973063.2805198/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTC  >  CP009273/607786‑608078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑