Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 607,931 G→A 100% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273607,9310GA100.0% 691.2 / NA 206S7F (TCC→TTC) fepAiron‑enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (117/89);  total (117/89)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAA  >  CP009273/607786‑608074
                                                                                                                                                 |                                                                                                                                               
    gTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGaaatgaa                                                                                                                                           <  2:654172/148‑1 (MQ=255)
     tGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAGATCCCCAGATTGACCAACAAGGCCAGGaaatgaat                                                                                                                                          <  1:2931177/148‑1 (MQ=255)
      gATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtc                                                                                                                                         >  1:933710/1‑148 (MQ=255)
        tGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtctt                                                                                                                                       <  2:3067488/148‑1 (MQ=255)
          gTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAAtcttct                                                                                                                                     <  1:1422707/148‑1 (MQ=255)
            cGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTg                                                                                                                                   >  2:831868/1‑148 (MQ=255)
               aaCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTc                                                                                                                                <  2:2667216/148‑1 (MQ=255)
                aCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCa                                                                                                                               <  2:729030/148‑1 (MQ=255)
                aCGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCa                                                                                                                               <  2:1371149/148‑1 (MQ=255)
                 cGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAt                                                                                                                              <  2:689308/148‑1 (MQ=255)
                  gCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCAtt                                                                                                                             <  1:815287/148‑1 (MQ=255)
                   ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTg                                                                                                                            >  2:1548696/1‑148 (MQ=255)
                   ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTg                                                                                                                            >  2:113423/1‑148 (MQ=255)
                   ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTg                                                                                                                            <  2:122894/148‑1 (MQ=255)
                   ccAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAACCTTCTTGTTCATTg                                                                                                                            <  1:2204024/148‑1 (MQ=255)
                    cAGGCGCCTGTAAGGTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGt                                                                                                                           <  2:1634636/148‑1 (MQ=255)
                     aGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtt                                                                                                                          >  2:2641177/1‑148 (MQ=255)
                      ggCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGttt                                                                                                                         >  1:3229071/1‑148 (MQ=255)
                       gcgcCTGTAAGTTCTGCTCGGCTGAGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTTTCTGTCGTGTCTTGTGCCTGAGCTACGCCATAAATCCCCAGTTTGACCTACTATGCCAGGATATGAATATTCTTGTTCATTGtttt                                                                                                                        <  2:2129203/148‑1 (MQ=255)
                       gcgcCTGTAAGTTCTGCTCGGCGGCGGTAACGACTATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                        <  2:33599/148‑1 (MQ=255)
                       gcgcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                        <  2:3262476/148‑1 (MQ=255)
                       gcgcCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGACTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGtttt                                                                                                                        >  1:2313854/1‑148 (MQ=255)
                          ccTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTAtt                                                                                                                     >  1:238482/1‑148 (MQ=255)
                          ccTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTAtt                                                                                                                     >  1:3241983/1‑148 (MQ=255)
                           cTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                    <  2:388013/148‑1 (MQ=255)
                           cTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                    <  2:388187/148‑1 (MQ=255)
                           cTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTc                                                                                                                    <  2:2492249/148‑1 (MQ=255)
                            tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTcc                                                                                                                   >  1:3138957/1‑148 (MQ=255)
                            tGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTcc                                                                                                                   >  1:468985/1‑148 (MQ=255)
                               aaGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTga                                                                                                                >  1:670392/1‑147 (MQ=255)
                               aaGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGc                                                                                                                >  1:87832/1‑148 (MQ=255)
                                aGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTGCATTGTTTTATTCCTGCa                                                                                                               >  2:3549708/1‑148 (MQ=255)
                                 gTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAt                                                                                                              <  2:1964856/148‑1 (MQ=255)
                                 gTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAt                                                                                                              >  2:3008192/1‑148 (MQ=255)
                                   tcttctCGGCGGCGGTAAAGACAATAGTATCGTCATGTGAACCTGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCACCACGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCtttt                                                                                                            <  2:2690207/148‑1 (MQ=255)
                                   tCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCAttt                                                                                                            >  1:164042/1‑148 (MQ=255)
                                         cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                      >  1:862539/1‑148 (MQ=255)
                                         cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                      >  2:1351762/1‑148 (MQ=255)
                                         cggcggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCa                                                                                                      >  2:2563072/1‑148 (MQ=255)
                                            cggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGa                                                                                                   >  2:2502937/1‑148 (MQ=255)
                                             ggcggTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGaa                                                                                                  >  1:2943372/1‑148 (MQ=255)
                                                   aaCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                                                            >  1:669998/1‑148 (MQ=255)
                                                   aaCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCaa                                                                                            >  1:3414555/1‑148 (MQ=255)
                                                    aCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAAc                                                                                           >  2:3248807/1‑148 (MQ=255)
                                                    aCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAAc                                                                                           >  2:3511817/1‑148 (MQ=255)
                                                    aCGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCGTGTGCCTGCGCTACCCCATAAAACCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGGTCATTGTTTTATTCCTGCATTTTTGCCCCGAAATGCAAc                                                                                           >  2:1750108/1‑148 (MQ=255)
                                                       aCAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGt                                                                                        <  1:1276734/148‑1 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                      >  1:3376364/1‑148 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                      >  2:1991639/1‑148 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                      >  1:1734050/1‑148 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                      >  1:956689/1‑148 (MQ=255)
                                                         aaTAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCg                                                                                      <  1:400343/148‑1 (MQ=255)
                                                            aGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGc                                                                                   >  2:379846/1‑148 (MQ=255)
                                                             gTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCa                                                                                  >  2:1086196/1‑148 (MQ=255)
                                                              tATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCAGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATAGTTTTATTCCTGCAATTTTGCCACGACTTGCAACTGTCGGGCAt                                                                                 >  2:206437/1‑148 (MQ=255)
                                                               aTCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATg                                                                                >  1:2100702/1‑148 (MQ=255)
                                                                 cGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgt                                                                              >  2:1288286/1‑148 (MQ=255)
                                                                     aTGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGgtcgtc                                                                          <  2:810209/148‑1 (MQ=255)
                                                                      tgtgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtca                                                                         >  2:3460589/1‑148 (MQ=255)
                                                                      tgtgAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTAATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGtca                                                                         >  2:1516467/1‑148 (MQ=255)
                                                                           aaCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAac                                                                    >  1:1024905/1‑148 (MQ=255)
                                                                            aCAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAaca                                                                   <  2:1917977/148‑1 (MQ=255)
                                                                                gAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACacgac                                                               >  2:3406742/1‑148 (MQ=255)
                                                                                  gTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGc                                                             >  1:1798690/1‑148 (MQ=255)
                                                                                     tcggtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATc                                                          <  2:455679/148‑1 (MQ=255)
                                                                                        gtcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCg                                                       >  1:2610921/1‑148 (MQ=255)
                                                                                         tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGc                                                      >  2:1827660/1‑148 (MQ=255)
                                                                                         tcggCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGc                                                      >  2:2158014/1‑148 (MQ=255)
                                                                                            gCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAc                                                   >  1:2033442/1‑148 (MQ=255)
                                                                                             ctctTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAcc                                                  >  1:1024389/1‑148 (MQ=255)
                                                                                                   tgCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaa                                            >  2:173998/1‑148 (MQ=255)
                                                                                                    gCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGaaaa                                           >  1:2539857/1‑148 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCt                                        <  2:87832/148‑1 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCt                                        >  1:65079/1‑148 (MQ=255)
                                                                                                       tGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCt                                        <  2:3229071/148‑1 (MQ=255)
                                                                                                         cgcTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCttt                                      <  2:933710/148‑1 (MQ=255)
                                                                                                          gcTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTg                                     <  1:113423/148‑1 (MQ=255)
                                                                                                          gatACCCCATAAATCCCCAGATTGACCAACAAGGCCAGTAAATGAATCTTCTTGTTCATTGTTTTATTCCTTCATTTTTGCCACGAATTGCAAATGTAGGGCATGGTCGTAATCAACACGACGCATCCCGCTACCGCGAAAACCTTTg                                     <  1:1548606/146‑1 (MQ=255)
                                                                                                           cTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGa                                    >  1:107063/1‑148 (MQ=255)
                                                                                                           cTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGa                                    >  1:1910286/1‑148 (MQ=255)
                                                                                                            tACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAt                                   <  1:1932441/148‑1 (MQ=255)
                                                                                                              ccccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTGTGATcc                                 >  2:2938688/1‑148 (MQ=255)
                                                                                                               cccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCt                                <  2:1777411/148‑1 (MQ=255)
                                                                                                                ccATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTg                               >  1:3136378/1‑148 (MQ=255)
                                                                                                                 cATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGa                              >  1:3066474/1‑148 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAg                           >  1:3255633/1‑148 (MQ=255)
                                                                                                                    aaaTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAg                           >  2:313120/1‑148 (MQ=255)
                                                                                                                      aTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGac                         <  1:3289289/148‑1 (MQ=255)
                                                                                                                      aTCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGac                         <  1:2661589/148‑1 (MQ=255)
                                                                                                                        ccccAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCCTCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGacac                       >  2:649645/1‑148 (MQ=255)
                                                                                                                           cAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACgca                    >  2:298846/1‑148 (MQ=255)
                                                                                                                                    caacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTg           >  2:1624446/1‑148 (MQ=255)
                                                                                                                                     aacaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTgg          >  2:166510/1‑148 (MQ=255)
                                                                                                                                      acaaGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGt         >  1:1819638/1‑148 (MQ=255)
                                                                                                                                          ggCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaat     >  1:3187762/1‑148 (MQ=255)
                                                                                                                                          ggCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaat     <  2:341352/148‑1 (MQ=255)
                                                                                                                                           gCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaatt    <  1:359159/148‑1 (MQ=255)
                                                                                                                                           gCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaatt    >  2:2347632/1‑148 (MQ=255)
                                                                                                                                             cAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaattaa  >  1:185708/1‑148 (MQ=255)
                                                                                                                                             cAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGttaattaa  <  2:1701141/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAA  >  CP009273/607786‑608074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCG  >  CP009273/607785‑608080
                                                                                                                                                  |                                                                                                                                                     
CTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAAT                                                                                                                                                    >  SRR19973066.937220/1‑150 (MQ=60)
     GTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATC                                                                                                                                               >  SRR19973066.940492/1‑150 (MQ=60)
      TGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAGATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCT                                                                                                                                              <  SRR19973066.2950686/150‑1 (MQ=60)
           GTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTG                                                                                                                                         <  SRR19973066.1432883/150‑1 (MQ=60)
             CGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTT                                                                                                                                       >  SRR19973066.654629/1‑150 (MQ=60)
              GAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTC                                                                                                                                      >  SRR19973066.1512491/1‑150 (MQ=60)
                   GCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGT                                                                                                                                 <  SRR19973066.821261/150‑1 (MQ=60)
                    CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTT                                                                                                                                <  SRR19973066.2219334/150‑1 (MQ=60)
                    CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTT                                                                                                                                <  SRR19973066.2957780/150‑1 (MQ=60)
                    CCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAACCTTCTTGTTCATTGTT                                                                                                                                <  SRR19973066.2219300/150‑1 (MQ=60)
                     CAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTT                                                                                                                               >  SRR19973066.3250397/1‑150 (MQ=60)
                     CAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTT                                                                                                                               >  SRR19973066.3250407/1‑150 (MQ=60)
                      AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                              >  SRR19973066.1231009/1‑150 (MQ=60)
                      AGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGACTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTT                                                                                                                              >  SRR19973066.2329687/1‑150 (MQ=60)
                         CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                           >  SRR19973066.3263370/1‑150 (MQ=60)
                         CGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATT                                                                                                                           >  SRR19973066.240355/1‑150 (MQ=60)
                           CNTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                         >  SRR19973066.3159901/1‑150 (MQ=60)
                           CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                         >  SRR19973066.3160012/1‑150 (MQ=60)
                           CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                         >  SRR19973066.472551/1‑150 (MQ=60)
                           CCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCC                                                                                                                         >  SRR19973066.472552/1‑150 (MQ=60)
                              GTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGC                                                                                                                      >  SRR19973066.88787/1‑150 (MQ=60)
                              GTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGC                                                                                                                      >  SRR19973066.387064/1‑150 (MQ=60)
                              GTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGC                                                                                                                      >  SRR19973066.88785/1‑150 (MQ=60)
                              GTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGA                                                                                                                      >  SRR19973066.675290/1‑150 (MQ=60)
                                  GTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTT                                                                                                                  >  SRR19973066.165525/1‑150 (MQ=60)
                                        CTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCA                                                                                                            >  SRR19973066.868745/1‑150 (MQ=60)
                                            GCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAA                                                                                                        >  SRR19973066.2962926/1‑150 (MQ=60)
                                                 GGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCA                                                                                                   <  SRR19973066.1311836/150‑1 (MQ=60)
                                                  GTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                  >  SRR19973066.674296/1‑150 (MQ=60)
                                                  GTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                  >  SRR19973066.3437386/1‑150 (MQ=60)
                                                  GTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                  >  SRR19973066.3437319/1‑150 (MQ=60)
                                                  GTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                  >  SRR19973066.674887/1‑150 (MQ=60)
                                                  GTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAA                                                                                                  >  SRR19973066.674899/1‑150 (MQ=60)
                                                    AACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACT                                                                                                >  SRR19973066.1109711/1‑150 (MQ=60)
                                                      CGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGT                                                                                              >  SRR19973066.2622706/1‑150 (MQ=60)
                                                        ACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCG                                                                                            >  SRR19973066.3398879/1‑150 (MQ=60)
                                                        ACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCG                                                                                            >  SRR19973066.963688/1‑150 (MQ=60)
                                                        ACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCG                                                                                            <  SRR19973066.1285950/150‑1 (MQ=60)
                                                        ACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCG                                                                                            >  SRR19973066.1746570/1‑150 (MQ=60)
                                                         CAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGG                                                                                           >  SRR19973066.2358430/1‑150 (MQ=60)
                                                          AATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGG                                                                                          <  SRR19973066.2211610/150‑1 (MQ=60)
                                                          AATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGG                                                                                          <  SRR19973066.403494/150‑1 (MQ=60)
                                                             AGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCAT                                                                                       >  SRR19973066.1979620/1‑150 (MQ=60)
                                                              GTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATG                                                                                      >  SRR19973066.2115164/1‑150 (MQ=60)
                                                                      ATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCAT                                                                              >  SRR19973066.2506305/1‑150 (MQ=60)
                                                                      ATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCAT                                                                              <  SRR19973066.797093/150‑1 (MQ=60)
                                                                       TGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATC                                                                             >  SRR19973066.761449/1‑150 (MQ=60)
                                                                          GAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAAC                                                                          >  SRR19973066.1032451/1‑150 (MQ=60)
                                                                          GAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAAC                                                                          >  SRR19973066.1032457/1‑150 (MQ=60)
                                                                               AGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCACCTGTCGGGCATGGTCGTCATCAACACGAC                                                                     >  SRR19973066.959117/1‑150 (MQ=60)
                                                                                GGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCGTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACG                                                                    <  SRR19973066.1936544/150‑1 (MQ=60)
                                                                                 GAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGC                                                                   >  SRR19973066.1811477/1‑150 (MQ=60)
                                                                                       CGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCG                                                             >  SRR19973066.2628569/1‑150 (MQ=60)
                                                                                       CGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCACAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCG                                                             >  SRR19973066.2627970/1‑150 (MQ=60)
                                                                                           CGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAC                                                         >  SRR19973066.3462690/1‑150 (MQ=60)
                                                                                           CGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAC                                                         >  SRR19973066.286569/1‑150 (MQ=60)
                                                                                           CGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTAC                                                         >  SRR19973066.2047630/1‑150 (MQ=60)
                                                                                            GGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACC                                                        >  SRR19973066.1032025/1‑150 (MQ=60)
                                                                                            GGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACC                                                        >  SRR19973066.1031929/1‑150 (MQ=60)
                                                                                                   GTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAA                                                 >  SRR19973066.2557119/1‑150 (MQ=60)
                                                                                                      CCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCT                                              >  SRR19973066.65875/1‑150 (MQ=60)
                                                                                                          CGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGA                                          >  SRR19973066.108170/1‑150 (MQ=60)
                                                                                                          CGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGA                                          >  SRR19973066.1923890/1‑150 (MQ=60)
                                                                                                          CGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGA                                          >  SRR19973066.1982747/1‑150 (MQ=60)
                                                                                                           GCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGATTTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAT                                         <  SRR19973066.1559787/150‑1 (MQ=60)
                                                                                                           GCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAT                                         <  SRR19973066.114582/150‑1 (MQ=60)
                                                                                                           GATACCCCATAAATCCCCAGATTGACCATCAAGGCCAGTAAATGAATCTTCTTGTTCATTGTTTTATTCCTTCATTTTTGCCACGAATTGCAAATGTAGGGCATGGTCGTAATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGAT                                         <  SRR19973066.1559697/150‑1 (MQ=60)
                                                                                                             TACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCC                                       <  SRR19973066.3184768/150‑1 (MQ=60)
                                                                                                             TACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCC                                       <  SRR19973066.1946156/150‑1 (MQ=60)
                                                                                                               CCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTG                                     >  SRR19973066.3157505/1‑150 (MQ=60)
                                                                                                               CCCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTG                                     >  SRR19973066.3157313/1‑150 (MQ=60)
                                                                                                                CCCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGA                                    >  SRR19973066.3086980/1‑150 (MQ=60)
                                                                                                                 CCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAA                                   >  SRR19973066.3480297/1‑150 (MQ=60)
                                                                                                                 CCATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAA                                   >  SRR19973066.3380187/1‑150 (MQ=60)
                                                                                                                  CATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAA                                  >  SRR19973066.1629523/1‑150 (MQ=60)
                                                                                                                   ATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAG                                 >  SRR19973066.3277052/1‑150 (MQ=60)
                                                                                                                   ATAAATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAG                                 >  SRR19973066.3277061/1‑150 (MQ=60)
                                                                                                                      AATCCCCAGATTGACCAACCAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACA                              <  SRR19973066.2641549/150‑1 (MQ=60)
                                                                                                                      AATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACA                              <  SRR19973066.837935/150‑1 (MQ=60)
                                                                                                                       ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                             <  SRR19973066.3310957/150‑1 (MQ=60)
                                                                                                                       ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                             <  SRR19973066.1901151/150‑1 (MQ=60)
                                                                                                                       ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                             >  SRR19973066.3086029/1‑150 (MQ=60)
                                                                                                                       ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                             <  SRR19973066.3310976/150‑1 (MQ=60)
                                                                                                                       ATCCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACAC                             <  SRR19973066.2679423/150‑1 (MQ=60)
                                                                                                                         CCCCAGATTGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGC                           >  SRR19973066.1685522/1‑150 (MQ=60)
                                                                                                                          CCCAGATTGACCAACAAGGCCAGGAAATGAACCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCA                          <  SRR19973066.175543/150‑1 (MQ=60)
                                                                                                                                 TGACCAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGNCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGT                   <  SRR19973066.1824969/150‑1 (MQ=60)
                                                                                                                                     CAACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGT               >  SRR19973066.1832606/1‑150 (MQ=60)
                                                                                                                                      AACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTT              >  SRR19973066.2088781/1‑150 (MQ=60)
                                                                                                                                       ACAAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTA             >  SRR19973066.3254118/1‑150 (MQ=60)
                                                                                                                                         AAGGCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAAT           >  SRR19973066.3208957/1‑150 (MQ=60)
                                                                                                                                            GCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAA        >  SRR19973066.187325/1‑150 (MQ=60)
                                                                                                                                            GCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAA        <  SRR19973066.361960/150‑1 (MQ=60)
                                                                                                                                            GCCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAAGTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTCATTAA        >  SRR19973066.1854213/1‑150 (MQ=60)
                                                                                                                                             CCAGGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAAT       <  SRR19973066.3156975/150‑1 (MQ=60)
                                                                                                                                                GGAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTC    >  SRR19973066.2473162/1‑150 (MQ=60)
                                                                                                                                                 GAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACACATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCC   <  SRR19973066.2818867/150‑1 (MQ=60)
                                                                                                                                                 GAAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACACATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCC   <  SRR19973066.2818818/150‑1 (MQ=60)
                                                                                                                                                  AAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCG  >  SRR19973066.3445084/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGGCTCTTGTGCCTGCGCTACCCCATAAATCCCCAGATTGACCAACAAGGCCAGGGAATGAATCTTCTTGTTCATTGTTTTATTCCTGCATTTTTGCCACGAATTGCAACTGTCGGGCATGGTCGTCATCAACACGACGCATCCCGCTACCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCG  >  CP009273/607785‑608080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑