Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 591,864 C→A 90.1% A270E (GCG→GAG)  cusC → copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273591,8640CA90.1% 426.4 / 43.9 172A270E (GCG→GAG) cusCcopper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base C (11/6);  new base A (75/80);  total (86/86)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 174 total aligned reads displayed.

GAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGC  >  CP009273/591719‑592005
                                                                                                                                                 |                                                                                                                                             
gAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACcggagg                                                                                                                                             <  1:3240987/148‑1 (MQ=255)
  aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGa                                                                                                                                           <  1:1032085/148‑1 (MQ=255)
  aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGa                                                                                                                                           <  1:2315588/148‑1 (MQ=255)
    gTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAtt                                                                                                                                         >  1:166310/1‑148 (MQ=255)
     tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTg                                                                                                                                        >  2:2937607/1‑148 (MQ=255)
     tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                        >  2:669096/1‑148 (MQ=255)
     tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                        >  1:3303306/1‑148 (MQ=255)
      aCCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgt                                                                                                                                       >  2:190907/1‑148 (MQ=255)
       cccGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtc                                                                                                                                      >  2:1788333/1‑148 (MQ=255)
        ccGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtcg                                                                                                                                     <  2:214362/148‑1 (MQ=255)
        ccGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtcg                                                                                                                                     <  2:214392/148‑1 (MQ=255)
           cAGCGACATCGCTACAAGTCAGGGGGAACTGGCGCAGGAGAATTATGCTTTGCAACTGTTGCTGTGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAATCAGCGACAGCCTGCAAAGCGTTTAATTACCGGAGGGATTGtcgtcg                                                                                                                                  <  2:1572500/148‑1 (MQ=255)
           cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                  <  2:1572125/148‑1 (MQ=255)
              cGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCaa                                                                                                                               >  1:2832602/1‑148 (MQ=255)
               gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCaaa                                                                                                                              <  1:2471186/148‑1 (MQ=255)
               gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCaaa                                                                                                                              <  1:2471151/148‑1 (MQ=255)
                aCATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAAt                                                                                                                             <  2:1393819/148‑1 (MQ=255)
                 cATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATc                                                                                                                            >  2:518438/1‑148 (MQ=255)
                   tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                          >  1:2818669/1‑148 (MQ=255)
                   tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                          >  1:2818624/1‑148 (MQ=255)
                    cGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTa                                                                                                                         >  2:1000589/1‑148 (MQ=255)
                     gCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAt                                                                                                                        >  1:265149/1‑148 (MQ=255)
                      cTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAtt                                                                                                                       >  2:1527072/1‑148 (MQ=255)
                       tAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTg                                                                                                                      <  1:435597/148‑1 (MQ=255)
                         aaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                    >  1:3149003/1‑148 (MQ=255)
                             tCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAgcgc                                                                                                                >  1:646843/1‑148 (MQ=255)
                                    gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGata                                                                                                         >  1:1837677/1‑148 (MQ=255)
                                      aCTGGCGCAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTCCCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatt                                                                                                       <  1:3072785/148‑1 (MQ=255)
                                      aCTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatt                                                                                                       <  2:1199476/148‑1 (MQ=255)
                                       cTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatta                                                                                                      <  2:2387169/148‑1 (MQ=255)
                                       cTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatta                                                                                                      <  2:1303366/148‑1 (MQ=255)
                                          gcgcCGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGAAAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATgg                                                                                                   <  1:1172101/148‑1 (MQ=255)
                                          gcgcAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATgg                                                                                                   >  2:600/1‑148 (MQ=255)
                                           cgcAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGa                                                                                                  <  1:997484/148‑1 (MQ=255)
                                           cgcAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGCCAAGCTGCCGCAAGCGCATACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGa                                                                                                  <  1:2518374/148‑1 (MQ=255)
                                             cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCACAGCGTTAAATTACCGCCGGGATTGTCTTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAg                                                                                                >  1:578786/1‑148 (MQ=255)
                                             cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAg                                                                                                <  1:190907/148‑1 (MQ=255)
                                               ggCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCt                                                                                              >  1:1282662/1‑148 (MQ=255)
                                               ggCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCt                                                                                              >  1:1282648/1‑148 (MQ=255)
                                                    ataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAaca                                                                                         >  2:3141443/1‑148 (MQ=255)
                                                    ataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAaca                                                                                         <  1:1317504/148‑1 (MQ=255)
                                                    ataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAaca                                                                                         >  2:436760/1‑148 (MQ=255)
                                                    ataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAaca                                                                                         >  2:1007616/1‑148 (MQ=255)
                                                      aatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcg                                                                                       >  1:2725499/1‑148 (MQ=255)
                                                       atGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcgc                                                                                      >  2:2206311/1‑148 (MQ=255)
                                                       atGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcgc                                                                                      >  1:535251/1‑148 (MQ=255)
                                                       atGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcgc                                                                                      >  2:2943525/1‑148 (MQ=255)
                                                           aTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTa                                                                                  <  2:900740/148‑1 (MQ=255)
                                                           aTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTa                                                                                  <  2:1838370/148‑1 (MQ=255)
                                                           aTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTa                                                                                  <  2:1408014/148‑1 (MQ=255)
                                                            ttGCAACTGTTGCTGGGAAGCTACGGCATGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTaa                                                                                 <  1:838648/148‑1 (MQ=255)
                                                            ttGCAACTGGTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTaa                                                                                 <  1:2885857/148‑1 (MQ=255)
                                                             tGCAAGTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAATTTACCGGAGGGTTTGTCGTCGAAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                <  1:2799393/148‑1 (MQ=255)
                                                             tGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                >  1:3156351/1‑148 (MQ=255)
                                                             tGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCAGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                <  1:2026405/148‑1 (MQ=255)
                                                                   tGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcggc                                                                          <  2:109972/148‑1 (MQ=255)
                                                                    gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCt                                                                         >  1:2231816/1‑148 (MQ=255)
                                                                     ttGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTa                                                                        >  1:833876/1‑148 (MQ=255)
                                                                       gCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAt                                                                      <  2:824609/148‑1 (MQ=255)
                                                                          gggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGcc                                                                   >  1:2976958/1‑148 (MQ=255)
                                                                            gAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCaa                                                                 <  2:2425841/148‑1 (MQ=255)
                                                                             aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                <  1:600/148‑1 (MQ=255)
                                                                             aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                >  1:3036492/1‑148 (MQ=255)
                                                                               gCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAatat                                                              <  1:3130000/148‑1 (MQ=255)
                                                                                cTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAtt                                                             >  1:337993/1‑148 (MQ=255)
                                                                                         gCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                    >  1:514595/1‑148 (MQ=255)
                                                                                           tGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCAcg                                                  <  2:146577/148‑1 (MQ=255)
                                                                                                   gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                          <  1:1002034/148‑1 (MQ=255)
                                                                                                   gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                          <  1:1000589/148‑1 (MQ=255)
                                                                                                   gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                          <  1:3251908/148‑1 (MQ=255)
                                                                                                   gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                          <  1:2878919/148‑1 (MQ=255)
                                                                                                   gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                          <  1:2223236/148‑1 (MQ=255)
                                                                                                        gACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttttt                                     >  2:384348/1‑148 (MQ=255)
                                                                                                         aCAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTc                                    >  2:2531859/1‑148 (MQ=255)
                                                                                                          cAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTcc                                   >  1:1242354/1‑148 (MQ=255)
                                                                                                            gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGt                                 <  1:518438/148‑1 (MQ=255)
                                                                                                            gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGt                                 <  2:1591135/148‑1 (MQ=255)
                                                                                                            gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGt                                 <  1:29073/148‑1 (MQ=255)
                                                                                                             tAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTc                                <  1:2047559/148‑1 (MQ=255)
                                                                                                              aaaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCt                               <  2:514582/148‑1 (MQ=255)
                                                                                                               aaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCta                              <  1:746992/148‑1 (MQ=255)
                                                                                                               aaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCta                              <  1:2405783/148‑1 (MQ=255)
                                                                                                                aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                             >  2:2503759/1‑148 (MQ=255)
                                                                                                                aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                             >  2:240595/1‑148 (MQ=255)
                                                                                                                    cGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGc                         <  2:422839/148‑1 (MQ=255)
                                                                                                                         gCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAc                    >  2:2370478/1‑148 (MQ=255)
                                                                                                                         gCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAc                    <  1:1154052/148‑1 (MQ=255)
                                                                                                                             gCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGc                <  1:2026118/148‑1 (MQ=255)
                                                                                                                             gCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGc                >  1:2177586/1‑148 (MQ=255)
                                                                                                                              cAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCg               <  1:2757864/148‑1 (MQ=255)
                                                                                                                                aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa             <  2:2780928/148‑1 (MQ=255)
                                                                                                                                aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa             >  1:1430407/1‑148 (MQ=255)
                                                                                                                                 aGCGTTAAATTACCGGAGGGTTTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaa            <  1:489337/148‑1 (MQ=255)
                                                                                                                                 aGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaa            >  1:2892203/1‑148 (MQ=255)
                                                                                                                                  gCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAat           >  2:2737019/1‑148 (MQ=255)
                                                                                                                                     ttAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATc        >  1:1785573/1‑148 (MQ=255)
                                                                                                                                     ttAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATc        >  1:113438/1‑148 (MQ=255)
                                                                                                                                      tAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCg       <  1:2875355/148‑1 (MQ=255)
                                                                                                                                         aTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGACTATCGAcc    >  2:1820284/1‑148 (MQ=255)
                                                                                                                                           tACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGc  >  2:2595532/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGC  >  CP009273/591719‑592005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  >  CP009273/591715‑592013
                                                                                                                                                     |                                                                                                                                                     
GATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGA                                                                                                                                                       >  SRR19973070.907122/1‑150 (MQ=60)
GATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGA                                                                                                                                                       >  SRR19973070.1810409/1‑150 (MQ=60)
GATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGA                                                                                                                                                       >  SRR19973070.1417592/1‑150 (MQ=60)
 ATACAACGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAAGTGTTGCTGGGAAGCTACGGCATGCTGCCGCAAGCGCAGACCGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                      <  SRR19973070.1421988/150‑1 (MQ=60)
  TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGG                                                                                                                                                     >  SRR19973070.2917013/1‑150 (MQ=60)
  TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973070.197819/150‑1 (MQ=60)
  TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973070.1144492/150‑1 (MQ=60)
  TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973070.2803944/150‑1 (MQ=60)
    GAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGA                                                                                                                                                   <  SRR19973070.3261890/150‑1 (MQ=60)
      AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 <  SRR19973070.1039384/150‑1 (MQ=60)
      AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 >  SRR19973070.167668/1‑150 (MQ=60)
      AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 <  SRR19973070.2330975/150‑1 (MQ=60)
      AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 <  SRR19973070.2427283/150‑1 (MQ=60)
      AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 <  SRR19973070.1039388/150‑1 (MQ=60)
       AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                                >  SRR19973070.3324459/1‑150 (MQ=60)
       AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                                >  SRR19973070.1058251/1‑150 (MQ=60)
                AGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAA                                                                                                                                       >  SRR19973070.2850932/1‑150 (MQ=60)
                   GACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATC                                                                                                                                    <  SRR19973070.2487292/150‑1 (MQ=60)
                   GACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATC                                                                                                                                    <  SRR19973070.2487256/150‑1 (MQ=60)
                     CATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTT                                                                                                                                  >  SRR19973070.2836891/1‑150 (MQ=60)
                     CATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTT                                                                                                                                  >  SRR19973070.2836845/1‑150 (MQ=60)
                       TCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAT                                                                                                                                >  SRR19973070.266908/1‑150 (MQ=60)
                        CGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATT                                                                                                                               <  SRR19973070.864697/150‑1 (MQ=60)
                           TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            >  SRR19973070.3169350/1‑150 (MQ=60)
                           TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            <  SRR19973070.2178481/150‑1 (MQ=60)
                           TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            <  SRR19973070.438759/150‑1 (MQ=60)
                            AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                           >  SRR19973070.2798799/1‑150 (MQ=60)
                               CGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGC                                                                                                                        >  SRR19973070.651459/1‑150 (MQ=60)
                               CGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGC                                                                                                                        >  SRR19973070.651058/1‑150 (MQ=60)
                                   AGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGG                                                                                                                    >  SRR19973070.205886/1‑150 (MQ=60)
                                   AGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGG                                                                                                                    >  SRR19973070.2867073/1‑150 (MQ=60)
                                      GGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATA                                                                                                                 >  SRR19973070.1850296/1‑150 (MQ=60)
                                          ACTGGCGCAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTCCCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTAT                                                                                                             <  SRR19973070.3092509/150‑1 (MQ=60)
                                              GCGCCGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGAAAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAA                                                                                                         <  SRR19973070.1180310/150‑1 (MQ=60)
                                              GCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCTTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAA                                                                                                         <  SRR19973070.437141/150‑1 (MQ=60)
                                              GCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAA                                                                                                         <  SRR19973070.2841758/150‑1 (MQ=60)
                                               CGCAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        <  SRR19973070.1004600/150‑1 (MQ=60)
                                               CGCAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGCCAAGCTGCCGCAAGCGCATACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        <  SRR19973070.2534787/150‑1 (MQ=60)
                                               CGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCACAGCGTTAAATTACCGCCGGGATTGTCTTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        >  SRR19973070.582767/1‑150 (MQ=60)
                                               CGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        <  SRR19973070.1004602/150‑1 (MQ=60)
                                               CGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        <  SRR19973070.129218/150‑1 (MQ=60)
                                                 CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                      >  SRR19973070.1291605/1‑150 (MQ=60)
                                                 CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                      <  SRR19973070.192378/150‑1 (MQ=60)
                                                 CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                      >  SRR19973070.1291619/1‑150 (MQ=60)
                                                 CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                      <  SRR19973070.192150/150‑1 (MQ=60)
                                                        ATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACG                                                                                               <  SRR19973070.1326606/150‑1 (MQ=60)
                                                        ATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACG                                                                                               >  SRR19973070.2743176/1‑150 (MQ=60)
                                                        ATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACG                                                                                               >  SRR19973070.2743081/1‑150 (MQ=60)
                                                        ATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACG                                                                                               <  SRR19973070.1326626/150‑1 (MQ=60)
                                                         TAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGC                                                                                              >  SRR19973070.3264835/1‑150 (MQ=60)
                                                         TAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGC                                                                                              >  SRR19973070.538846/1‑150 (MQ=60)
                                                           ATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGT                                                                                            >  SRR19973070.2489696/1‑150 (MQ=60)
                                                            TGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTT                                                                                           >  SRR19973070.2294788/1‑150 (MQ=60)
                                                               ATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAT                                                                                        >  SRR19973070.3176753/1‑150 (MQ=60)
                                                                TTGCAACTGTTGCTGGGAAGCTACGGCATGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATG                                                                                       <  SRR19973070.844314/150‑1 (MQ=60)
                                                                TTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATG                                                                                       <  SRR19973070.3098226/150‑1 (MQ=60)
                                                                TTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATG                                                                                       <  SRR19973070.3198932/150‑1 (MQ=60)
                                                                TTGCAACTGGTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATG                                                                                       <  SRR19973070.2904539/150‑1 (MQ=60)
                                                                 TGCAAGTTTTGATGGGAAGCTACGGCAAGCTGGGGAAAGCGAAGACATTAAACAGCGACAGCGTGCAAAGCTTTAAATTACCGGAGGGATTGTCGTGGAAAATCTTATTGAAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGG                                                                                      <  SRR19973070.1520197/150‑1 (MQ=60)
                                                                 TGCAAGTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAATTTACCGGAGGGTTTGTCGTCGAAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGG                                                                                      <  SRR19973070.2817472/150‑1 (MQ=60)
                                                                 TGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCAGGATATTATGGAAGCTGAACACGCGTTAATGG                                                                                      <  SRR19973070.2040111/150‑1 (MQ=60)
                                                                  GCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGC                                                                                     <  SRR19973070.683243/150‑1 (MQ=60)
                                                                      CTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCT                                                                                 >  SRR19973070.2246520/1‑150 (MQ=60)
                                                                       TGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTA                                                                                >  SRR19973070.839521/1‑150 (MQ=60)
                                                                       TGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTA                                                                                >  SRR19973070.545502/1‑150 (MQ=60)
                                                                         TTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAT                                                                              <  SRR19973070.2956539/150‑1 (MQ=60)
                                                                            CTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCC                                                                           >  SRR19973070.182710/1‑150 (MQ=60)
                                                                            CTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCC                                                                           >  SRR19973070.2996068/1‑150 (MQ=60)
                                                                               GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                        >  SRR19973070.3055802/1‑150 (MQ=60)
                                                                                 ATGCTAGGGCAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                      <  SRR19973070.612/150‑1 (MQ=60)
                                                                                  AGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATT                                                                     >  SRR19973070.340458/1‑150 (MQ=60)
                                                                                   GCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTG                                                                    >  SRR19973070.2897355/1‑150 (MQ=60)
                                                                                   GCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTG                                                                    >  SRR19973070.1238874/1‑150 (MQ=60)
                                                                                   GCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTG                                                                    <  SRR19973070.3150219/150‑1 (MQ=60)
                                                                                           AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973070.527447/1‑150 (MQ=60)
                                                                                           AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973070.518123/1‑150 (MQ=60)
                                                                                           AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973070.518109/1‑150 (MQ=60)
                                                                                                    CAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCA                                                   >  SRR19973070.254121/1‑150 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.1007720/150‑1 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.1009174/150‑1 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.2237787/150‑1 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.3272861/150‑1 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.2897564/150‑1 (MQ=60)
                                                                                                       GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                                <  SRR19973070.2237830/150‑1 (MQ=60)
                                                                                                           AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                            >  SRR19973070.2828336/1‑150 (MQ=60)
                                                                                                            GACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCC                                           >  SRR19973070.1251062/1‑150 (MQ=60)
                                                                                                            GACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCC                                           >  SRR19973070.2909463/1‑150 (MQ=60)
                                                                                                                GTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCT                                       <  SRR19973070.521977/150‑1 (MQ=60)
                                                                                                                GTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCT                                       <  SRR19973070.328504/150‑1 (MQ=60)
                                                                                                                GTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCT                                       <  SRR19973070.29460/150‑1 (MQ=60)
                                                                                                                 TAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTA                                      <  SRR19973070.2061408/150‑1 (MQ=60)
                                                                                                                   AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    >  SRR19973070.2395601/1‑150 (MQ=60)
                                                                                                                   AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    <  SRR19973070.752268/150‑1 (MQ=60)
                                                                                                                   AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    <  SRR19973070.2421654/150‑1 (MQ=60)
                                                                                                                     CAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAG                                  <  SRR19973070.3091118/150‑1 (MQ=60)
                                                                                                                         GACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTG                              >  SRR19973070.3046271/1‑150 (MQ=60)
                                                                                                                             GCCTGCAAAGCTTTAATTTACCGGTGTGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCA                          <  SRR19973070.3161742/150‑1 (MQ=60)
                                                                                                                             GCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCA                          <  SRR19973070.1162104/150‑1 (MQ=60)
                                                                                                                             GCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCA                          <  SRR19973070.3161744/150‑1 (MQ=60)
                                                                                                                               CTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGC                        >  SRR19973070.1430395/1‑150 (MQ=60)
                                                                                                                               CTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGC                        >  SRR19973070.2192006/1‑150 (MQ=60)
                                                                                                                                 GCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGG                      <  SRR19973070.2039824/150‑1 (MQ=60)
                                                                                                                                  CAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGA                     >  SRR19973070.47924/1‑150 (MQ=60)
                                                                                                                                  CAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGA                     <  SRR19973070.2775559/150‑1 (MQ=60)
                                                                                                                                  CAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGA                     >  SRR19973070.1440284/1‑150 (MQ=60)
                                                                                                                                   AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                    >  SRR19973070.2910920/1‑150 (MQ=60)
                                                                                                                                   AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                    >  SRR19973070.1179634/1‑150 (MQ=60)
                                                                                                                                   AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                    >  SRR19973070.793645/1‑150 (MQ=60)
                                                                                                                                     AGCGTTAAATTACCGGAGGGTTTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATA                  <  SRR19973070.492768/150‑1 (MQ=60)
                                                                                                                                       CGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATC                >  SRR19973070.1797920/1‑150 (MQ=60)
                                                                                                                                       CGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATC                >  SRR19973070.114482/1‑150 (MQ=60)
                                                                                                                                        GTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCG               >  SRR19973070.2024787/1‑150 (MQ=60)
                                                                                                                                          TAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAC             <  SRR19973070.2893979/150‑1 (MQ=60)
                                                                                                                                                  CGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA     >  SRR19973070.3235056/1‑150 (MQ=60)
                                                                                                                                                  CGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA     >  SRR19973070.2645154/1‑150 (MQ=60)
                                                                                                                                                     AGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  <  SRR19973070.2266759/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  >  CP009273/591715‑592013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑