Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 591,864 C→A A270E (GCG→GAG)  cusC → copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273591,8640CA99.6% 815.8 / NA 245A270E (GCG→GAG) cusCcopper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (119/125);  total (119/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA  >  CP009273/591717‑592010
                                                                                                                                                   |                                                                                                                                                  
tAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACcggagg                                                                                                                                                  <  1:1709767/150‑1 (MQ=255)
tAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACcggagg                                                                                                                                                  <  1:1678151/150‑1 (MQ=255)
  gAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGa                                                                                                                                                <  2:2163723/150‑1 (MQ=255)
   aaaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               <  1:2290435/150‑1 (MQ=255)
   aaaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                               >  2:2362214/1‑150 (MQ=255)
    aaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAtt                                                                                                                                              <  2:2998330/150‑1 (MQ=255)
     aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                             <  2:524506/150‑1 (MQ=255)
     aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                             <  1:1286270/150‑1 (MQ=255)
     aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                             <  1:2720830/150‑1 (MQ=255)
       tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTgtc                                                                                                                                           <  2:1882051/150‑1 (MQ=255)
           cGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                       <  2:2021092/150‑1 (MQ=255)
           cGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                       <  2:3351/150‑1 (MQ=255)
           cGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                       <  2:2804677/150‑1 (MQ=255)
           cGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGAATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                       <  2:3170979/150‑1 (MQ=255)
             cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCACAGCGTTAAATTACCGGAGGGATTGTCGTCGCa                                                                                                                                     >  1:1803913/1‑150 (MQ=255)
               gCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCaaa                                                                                                                                   <  1:110315/150‑1 (MQ=255)
                  aCATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCt                                                                                                                                <  1:2125945/150‑1 (MQ=255)
                    aTCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTa                                                                                                                              >  1:3287468/1‑150 (MQ=255)
                     tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAt                                                                                                                             <  2:2524915/150‑1 (MQ=255)
                      cGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAtt                                                                                                                            <  1:2351701/150‑1 (MQ=255)
                      cGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAtt                                                                                                                            <  1:509860/150‑1 (MQ=255)
                       gCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTg                                                                                                                           <  1:1004666/150‑1 (MQ=255)
                       gCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTg                                                                                                                           <  2:3219851/150‑1 (MQ=255)
                         tAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                         <  1:2960465/150‑1 (MQ=255)
                         tAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                         >  1:3723383/1‑150 (MQ=255)
                          aaaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAg                                                                                                                        <  1:2811036/150‑1 (MQ=255)
                               tCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGccc                                                                                                                   <  2:1365785/150‑1 (MQ=255)
                                 aGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCgg                                                                                                                 >  2:1600224/1‑150 (MQ=255)
                                 aGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCgg                                                                                                                 >  1:1531047/1‑150 (MQ=255)
                                 aGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCgg                                                                                                                 >  1:1275242/1‑150 (MQ=255)
                                    gggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGata                                                                                                              >  1:2605077/1‑150 (MQ=255)
                                    gggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGata                                                                                                              <  1:2873529/150‑1 (MQ=255)
                                    gggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGata                                                                                                              >  2:1205259/1‑150 (MQ=255)
                                      gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatt                                                                                                            >  2:2047120/1‑150 (MQ=255)
                                      gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatt                                                                                                            >  2:1094215/1‑150 (MQ=255)
                                           ggCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGa                                                                                                       <  2:3460013/150‑1 (MQ=255)
                                              gcAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGc                                                                                                    <  1:3504921/150‑1 (MQ=255)
                                                aGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTg                                                                                                  >  1:3408535/1‑150 (MQ=255)
                                                   cGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAac                                                                                               >  1:751071/1‑150 (MQ=255)
                                                     aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAacac                                                                                             <  1:1606297/150‑1 (MQ=255)
                                                     aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAacac                                                                                             <  1:1134028/150‑1 (MQ=255)
                                                     aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAacac                                                                                             <  1:1607253/150‑1 (MQ=255)
                                                     aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAacac                                                                                             >  2:172173/1‑150 (MQ=255)
                                                      ataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcg                                                                                            >  2:2300657/1‑150 (MQ=255)
                                                          tGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGtt                                                                                        <  1:2067277/150‑1 (MQ=255)
                                                             aTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                     <  2:1227116/150‑1 (MQ=255)
                                                              ttGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATg                                                                                    <  2:2062435/150‑1 (MQ=255)
                                                                 cAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCCAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcg                                                                                 >  2:1905848/1‑150 (MQ=255)
                                                                 cAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcg                                                                                 <  2:1667285/150‑1 (MQ=255)
                                                                   aCTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATggcggc                                                                               <  1:2496020/150‑1 (MQ=255)
                                                                    cTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCt                                                                              <  1:3341534/150‑1 (MQ=255)
                                                                    cTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCt                                                                              <  2:3762247/150‑1 (MQ=255)
                                                                     tGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTa                                                                             >  2:3598284/1‑150 (MQ=255)
                                                                      gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTaa                                                                            >  2:2013128/1‑150 (MQ=255)
                                                                      gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTaa                                                                            >  2:100543/1‑150 (MQ=255)
                                                                      gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTaa                                                                            <  1:803620/150‑1 (MQ=255)
                                                                       ttGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGATTGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAt                                                                           <  1:708228/150‑1 (MQ=255)
                                                                        tGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATg                                                                          <  1:3713246/150‑1 (MQ=255)
                                                                        tGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATg                                                                          >  2:56236/1‑150 (MQ=255)
                                                                          cTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGcc                                                                        <  1:1211957/150‑1 (MQ=255)
                                                                           tGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCa                                                                       >  1:309539/1‑150 (MQ=255)
                                                                            gggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTGATGGCCGCGCCCGGATAGTATGGTAGCTGAACACGCGGTAATGGCGGCTAATGCCaa                                                                      >  2:3741117/1‑150 (MQ=255)
                                                                             ggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                     >  1:2206532/1‑150 (MQ=255)
                                                                              gAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAata                                                                    >  2:1017486/1‑150 (MQ=255)
                                                                               aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAatat                                                                   >  2:2879919/1‑150 (MQ=255)
                                                                                aGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAtt                                                                  <  1:221716/150‑1 (MQ=255)
                                                                                aGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAtt                                                                  >  1:1834869/1‑150 (MQ=255)
                                                                                 gCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACCGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTg                                                                 <  2:3664346/150‑1 (MQ=255)
                                                                                   tACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgc                                                               <  1:365671/150‑1 (MQ=255)
                                                                                    aCGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcg                                                              >  1:470207/1‑150 (MQ=255)
                                                                                     cGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcgc                                                             >  1:1894637/1‑150 (MQ=255)
                                                                                     cGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcgc                                                             >  2:2723790/1‑150 (MQ=255)
                                                                                      ggCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCt                                                            >  1:1155565/1‑150 (MQ=255)
                                                                                         aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                         >  2:1732041/1‑150 (MQ=255)
                                                                                         aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                         >  1:3635057/1‑150 (MQ=255)
                                                                                         aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                         >  1:3634844/1‑150 (MQ=255)
                                                                                           gCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCAcg                                                       >  2:1643446/1‑150 (MQ=255)
                                                                                             tGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCAcgcg                                                     <  2:937646/150‑1 (MQ=255)
                                                                                                 gCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGc                                                 <  2:3503688/150‑1 (MQ=255)
                                                                                                         aGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTc                                         <  2:1421294/150‑1 (MQ=255)
                                                                                                         aGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTc                                         <  1:619990/150‑1 (MQ=255)
                                                                                                           aCAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCg                                       >  1:1423616/1‑150 (MQ=255)
                                                                                                                aaaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                                  >  1:3440625/1‑150 (MQ=255)
                                                                                                                aaaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                                  >  2:1673041/1‑150 (MQ=255)
                                                                                                                 aaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtata                                 <  2:2605077/150‑1 (MQ=255)
                                                                                                                 aaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtata                                 <  1:3571341/150‑1 (MQ=255)
                                                                                                                   cAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAg                               <  1:3200556/150‑1 (MQ=255)
                                                                                                                   cAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAg                               >  2:268236/1‑150 (MQ=255)
                                                                                                                              tGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCg                    >  2:469248/1‑150 (MQ=255)
                                                                                                                                cAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa                  >  1:3531628/1‑150 (MQ=255)
                                                                                                                                 aaaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGaa                 <  1:3328492/150‑1 (MQ=255)
                                                                                                                                  aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAat                >  2:1756499/1‑150 (MQ=255)
                                                                                                                                   aGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAata               >  2:3690902/1‑150 (MQ=255)
                                                                                                                                     cGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTGTTTCCGTCTATAAGCCTGACCAGCGGAATATc             <  2:1803913/150‑1 (MQ=255)
                                                                                                                                        tAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAc          <  1:2441268/150‑1 (MQ=255)
                                                                                                                                          aaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCg        <  2:673623/150‑1 (MQ=255)
                                                                                                                                           aTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGc       >  2:71180/1‑150 (MQ=255)
                                                                                                                                            ttACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGcc      >  2:3075298/1‑150 (MQ=255)
                                                                                                                                            ttACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGcc      >  2:417372/1‑150 (MQ=255)
                                                                                                                                                cggaggGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTa  <  1:1017486/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA  >  CP009273/591717‑592010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  >  CP009273/591717‑592013
                                                                                                                                                   |                                                                                                                                                     
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973061.1682364/150‑1 (MQ=60)
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973061.1871627/150‑1 (MQ=60)
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     <  SRR19973061.1714062/150‑1 (MQ=60)
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACGGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGG                                                                                                                                                     >  SRR19973061.3606956/1‑150 (MQ=60)
   AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAT                                                                                                                                                  <  SRR19973061.2296398/150‑1 (MQ=60)
   AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAT                                                                                                                                                  >  SRR19973061.3746905/1‑150 (MQ=60)
   AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAT                                                                                                                                                  <  SRR19973061.2296227/150‑1 (MQ=60)
    AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                                 <  SRR19973061.207210/150‑1 (MQ=60)
     AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                                <  SRR19973061.2727832/150‑1 (MQ=60)
     AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                                <  SRR19973061.1289438/150‑1 (MQ=60)
     AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                                <  SRR19973061.1289459/150‑1 (MQ=60)
        ACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCG                                                                                                                                             >  SRR19973061.3309295/1‑150 (MQ=60)
        ACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCG                                                                                                                                             >  SRR19973061.3309299/1‑150 (MQ=60)
         CCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGT                                                                                                                                            >  SRR19973061.1820270/1‑150 (MQ=60)
          CCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTC                                                                                                                                           <  SRR19973061.2346002/150‑1 (MQ=60)
             CAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCACAGCGTTAAATTACCGGAGGGATTGTCGTCGCA                                                                                                                                        >  SRR19973061.1808415/1‑150 (MQ=60)
               GCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAA                                                                                                                                      <  SRR19973061.110682/150‑1 (MQ=60)
                CGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAAT                                                                                                                                     >  SRR19973061.553081/1‑150 (MQ=60)
                  ACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCT                                                                                                                                   <  SRR19973061.2131283/150‑1 (MQ=60)
                    ATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTA                                                                                                                                 >  SRR19973061.3295936/1‑150 (MQ=60)
                      CGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCTTCGCAAATCTTATT                                                                                                                               >  SRR19973061.964204/1‑150 (MQ=60)
                      CGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATT                                                                                                                               <  SRR19973061.2357679/150‑1 (MQ=60)
                      CGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATT                                                                                                                               <  SRR19973061.511205/150‑1 (MQ=60)
                       GCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTG                                                                                                                              <  SRR19973061.1007225/150‑1 (MQ=60)
                         TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            >  SRR19973061.3732901/1‑150 (MQ=60)
                         TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            <  SRR19973061.2968105/150‑1 (MQ=60)
                         TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            <  SRR19973061.2967063/150‑1 (MQ=60)
                         TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            >  SRR19973061.1342406/1‑150 (MQ=60)
                         TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGAATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGAAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                            >  SRR19973061.1536838/1‑150 (MQ=60)
                          AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                           <  SRR19973061.2818250/150‑1 (MQ=60)
                          AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                           <  SRR19973061.2818160/150‑1 (MQ=60)
                          AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                           >  SRR19973061.1737929/1‑150 (MQ=60)
                              GTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCC                                                                                                                       <  SRR19973061.79269/150‑1 (MQ=60)
                              GTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCC                                                                                                                       >  SRR19973061.599604/1‑150 (MQ=60)
                                CAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCG                                                                                                                     >  SRR19973061.131309/1‑150 (MQ=60)
                                 AGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGG                                                                                                                    >  SRR19973061.1534864/1‑150 (MQ=60)
                                 AGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGG                                                                                                                    >  SRR19973061.1278384/1‑150 (MQ=60)
                                 AGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGG                                                                                                                    <  SRR19973061.2273516/150‑1 (MQ=60)
                                    GGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATC                                                                                                                 >  SRR19973061.2611772/1‑150 (MQ=60)
                                    GGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATA                                                                                                                 <  SRR19973061.2880895/150‑1 (MQ=60)
                                     GGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATAT                                                                                                                >  SRR19973061.718011/1‑150 (MQ=60)
                                     GGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATAT                                                                                                                <  SRR19973061.3257768/150‑1 (MQ=60)
                                      GAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATT                                                                                                               >  SRR19973061.1224514/1‑150 (MQ=60)
                                       AACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTA                                                                                                              >  SRR19973061.675369/1‑150 (MQ=60)
                                         CTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGTGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATG                                                                                                            <  SRR19973061.3565611/150‑1 (MQ=60)
                                         CTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATG                                                                                                            <  SRR19973061.3565608/150‑1 (MQ=60)
                                           GGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGA                                                                                                          >  SRR19973061.2585379/1‑150 (MQ=60)
                                            GCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAA                                                                                                         <  SRR19973061.2886006/150‑1 (MQ=60)
                                             CGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAG                                                                                                        >  SRR19973061.1610448/1‑150 (MQ=60)
                                              GCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGC                                                                                                       <  SRR19973061.3513888/150‑1 (MQ=60)
                                                AGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTG                                                                                                     >  SRR19973061.3417289/1‑150 (MQ=60)
                                                   CGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAAC                                                                                                  >  SRR19973061.3073595/1‑150 (MQ=60)
                                                   CGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAAC                                                                                                  >  SRR19973061.753046/1‑150 (MQ=60)
                                                   CGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAAC                                                                                                  >  SRR19973061.3181042/1‑150 (MQ=60)
                                                    GAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACA                                                                                                 <  SRR19973061.2798800/150‑1 (MQ=60)
                                                    GAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACA                                                                                                 >  SRR19973061.78524/1‑150 (MQ=60)
                                                     AATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAC                                                                                                <  SRR19973061.1611252/150‑1 (MQ=60)
                                                     AATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAC                                                                                                <  SRR19973061.1610296/150‑1 (MQ=60)
                                                     AATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAC                                                                                                <  SRR19973061.1136920/150‑1 (MQ=60)
                                                     AATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAC                                                                                                <  SRR19973061.1136768/150‑1 (MQ=60)
                                                          TGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTT                                                                                           <  SRR19973061.2072477/150‑1 (MQ=60)
                                                          TGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTAAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTT                                                                                           <  SRR19973061.3664443/150‑1 (MQ=60)
                                                           GCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTA                                                                                          <  SRR19973061.1853784/150‑1 (MQ=60)
                                                            CATTGCAACTGTTGCTGGGAAGCTACTGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAA                                                                                         >  SRR19973061.2374115/1‑150 (MQ=60)
                                                              TTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATG                                                                                       <  SRR19973061.1717312/150‑1 (MQ=60)
                                                               TGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGG                                                                                      >  SRR19973061.1263871/1‑150 (MQ=60)
                                                                 CAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCG                                                                                    >  SRR19973061.2078044/1‑150 (MQ=60)
                                                                  AACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGG                                                                                   >  SRR19973061.1032847/1‑150 (MQ=60)
                                                                   ACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGC                                                                                  <  SRR19973061.2502376/150‑1 (MQ=60)
                                                                    CTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCT                                                                                 <  SRR19973061.3350136/150‑1 (MQ=60)
                                                                     TGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTA                                                                                <  SRR19973061.473100/150‑1 (MQ=60)
                                                                      GTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAA                                                                               <  SRR19973061.805728/150‑1 (MQ=60)
                                                                       GTGCTGGGAAGCTACGGAAAGCTGCCGCTAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGATTGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAT                                                                              <  SRR19973061.710075/150‑1 (MQ=60)
                                                                        TGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATG                                                                             >  SRR19973061.2460090/1‑150 (MQ=60)
                                                                        TGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATG                                                                             <  SRR19973061.3722738/150‑1 (MQ=60)
                                                                        TGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATG                                                                             <  SRR19973061.3722737/150‑1 (MQ=60)
                                                                          CTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCC                                                                           <  SRR19973061.1214995/150‑1 (MQ=60)
                                                                           TGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCA                                                                          >  SRR19973061.310352/1‑150 (MQ=60)
                                                                           TGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCA                                                                          >  SRR19973061.347407/1‑150 (MQ=60)
                                                                             GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                        <  SRR19973061.1619466/150‑1 (MQ=60)
                                                                             GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                        <  SRR19973061.1620189/150‑1 (MQ=60)
                                                                             GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                        >  SRR19973061.2212077/1‑150 (MQ=60)
                                                                                AGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATT                                                                     <  SRR19973061.222366/150‑1 (MQ=60)
                                                                                AGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATT                                                                     >  SRR19973061.1839466/1‑150 (MQ=60)
                                                                                   TACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGC                                                                  <  SRR19973061.366663/150‑1 (MQ=60)
                                                                                    ACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCG                                                                 >  SRR19973061.471477/1‑150 (MQ=60)
                                                                                    ACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCG                                                                 <  SRR19973061.3428515/150‑1 (MQ=60)
                                                                                     CGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGC                                                                >  SRR19973061.1899338/1‑150 (MQ=60)
                                                                                      GGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCT                                                               >  SRR19973061.2619386/1‑150 (MQ=60)
                                                                                      GGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCT                                                               >  SRR19973061.1158468/1‑150 (MQ=60)
                                                                                      GGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCT                                                               >  SRR19973061.1158497/1‑150 (MQ=60)
                                                                                        CAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGC                                                             >  SRR19973061.3794090/1‑150 (MQ=60)
                                                                                        CAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGC                                                             >  SRR19973061.3708437/1‑150 (MQ=60)
                                                                                         AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973061.3644349/1‑150 (MQ=60)
                                                                                         AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973061.3644342/1‑150 (MQ=60)
                                                                                         AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973061.3644136/1‑150 (MQ=60)
                                                                                         AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                            >  SRR19973061.3644212/1‑150 (MQ=60)
                                                                                            CTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGC                                                         >  SRR19973061.1158446/1‑150 (MQ=60)
                                                                                              GCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGC                                                       <  SRR19973061.1604199/150‑1 (MQ=60)
                                                                                              GCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGC                                                       >  SRR19973061.2218558/1‑150 (MQ=60)
                                                                                               CCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCG                                                      <  SRR19973061.2872253/150‑1 (MQ=60)
                                                                                                  CAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCA                                                   >  SRR19973061.3533461/1‑150 (MQ=60)
                                                                                                      CGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTT                                               >  SRR19973061.3471813/1‑150 (MQ=60)
                                                                                                         AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                            <  SRR19973061.621574/150‑1 (MQ=60)
                                                                                                         AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                            >  SRR19973061.2159961/1‑150 (MQ=60)
                                                                                                         AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                            <  SRR19973061.3352157/150‑1 (MQ=60)
                                                                                                           ACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCG                                          >  SRR19973061.1427203/1‑150 (MQ=60)
                                                                                                                AAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTAT                                     >  SRR19973061.3449454/1‑150 (MQ=60)
                                                                                                                 AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    <  SRR19973061.1687823/150‑1 (MQ=60)
                                                                                                                 AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    <  SRR19973061.3580492/150‑1 (MQ=60)
                                                                                                                 AACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATA                                    <  SRR19973061.1208284/150‑1 (MQ=60)
                                                                                                                  ACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAA                                   <  SRR19973061.210288/150‑1 (MQ=60)
                                                                                                                   CAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAG                                  <  SRR19973061.1472020/150‑1 (MQ=60)
                                                                                                                   CAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAG                                  <  SRR19973061.2219435/150‑1 (MQ=60)
                                                                                                                   CAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAG                                  <  SRR19973061.3208778/150‑1 (MQ=60)
                                                                                                                            CCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAG                         <  SRR19973061.3398218/150‑1 (MQ=60)
                                                                                                                                CAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGA                     >  SRR19973061.3706704/1‑150 (MQ=60)
                                                                                                                                CAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGA                     >  SRR19973061.3540671/1‑150 (MQ=60)
                                                                                                                                 AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                    <  SRR19973061.3337070/150‑1 (MQ=60)
                                                                                                                                 AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                    <  SRR19973061.3337536/150‑1 (MQ=60)
                                                                                                                                    GCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATAT                 <  SRR19973061.740193/150‑1 (MQ=60)
                                                                                                                                      GTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCG               <  SRR19973061.2561351/150‑1 (MQ=60)
                                                                                                                                      GTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCG               <  SRR19973061.1039105/150‑1 (MQ=60)
                                                                                                                                        TAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAC             <  SRR19973061.2447492/150‑1 (MQ=60)
                                                                                                                                        TAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAC             <  SRR19973061.2447531/150‑1 (MQ=60)
                                                                                                                                        TAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAC             >  SRR19973061.3115780/1‑150 (MQ=60)
                                                                                                                                                CGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA     <  SRR19973061.1018635/150‑1 (MQ=60)
                                                                                                                                                CGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTA     <  SRR19973061.1020078/150‑1 (MQ=60)
                                                                                                                                                 GGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAG    >  SRR19973061.1732151/1‑150 (MQ=60)
                                                                                                                                                  GAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGC   <  SRR19973061.1351941/150‑1 (MQ=60)
                                                                                                                                                   AGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGTGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  <  SRR19973061.56458/150‑1 (MQ=60)
                                                                                                                                                   AGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  <  SRR19973061.56433/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAGCG  >  CP009273/591717‑592013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑