Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 591,864 C→A 100% A270E (GCG→GAG)  cusC → copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273591,8640CA99.5% 685.9 / ‑4.3 212A270E (GCG→GAG) cusCcopper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (108/102);  total (108/104)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCA  >  CP009273/591720‑592007
                                                                                                                                                |                                                                                                                                               
aaaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAggg                                                                                                                                              >  1:2244385/1‑148 (MQ=255)
aaaGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAggg                                                                                                                                              <  2:2857014/148‑1 (MQ=255)
  aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAt                                                                                                                                            >  1:3637190/1‑148 (MQ=255)
   gTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAtt                                                                                                                                           >  2:3574945/1‑148 (MQ=255)
    tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTg                                                                                                                                          >  1:353733/1‑148 (MQ=255)
          cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                    <  1:2908529/148‑1 (MQ=255)
          cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                    <  1:2601157/148‑1 (MQ=255)
          cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                    >  2:3789595/1‑148 (MQ=255)
          cAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGtcgtcg                                                                                                                                    >  1:3752476/1‑148 (MQ=255)
               aCATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAAt                                                                                                                               <  1:586540/148‑1 (MQ=255)
                  tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                            <  1:806410/148‑1 (MQ=255)
                  tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                            <  1:2841189/148‑1 (MQ=255)
                  tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                            >  2:3615840/1‑148 (MQ=255)
                  tCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCtt                                                                                                                            <  2:592783/148‑1 (MQ=255)
                    gCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAt                                                                                                                          <  2:2164856/148‑1 (MQ=255)
                     cTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAtt                                                                                                                         >  1:457864/1‑148 (MQ=255)
                        aaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                      >  1:914818/1‑148 (MQ=255)
                        aaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCa                                                                                                                      >  1:446000/1‑148 (MQ=255)
                         aCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAg                                                                                                                     >  1:1913166/1‑148 (MQ=255)
                          cGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGAAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAgc                                                                                                                    >  2:1352043/1‑148 (MQ=255)
                             cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGcc                                                                                                                 >  2:3242685/1‑148 (MQ=255)
                             cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGcc                                                                                                                 >  1:2289828/1‑148 (MQ=255)
                             cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGAAGCGcc                                                                                                                 >  1:362879/1‑148 (MQ=255)
                                ggggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCgg                                                                                                              >  2:1724005/1‑148 (MQ=255)
                                  ggAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGat                                                                                                            <  1:3235408/148‑1 (MQ=255)
                                   gAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGata                                                                                                           <  1:860610/148‑1 (MQ=255)
                                     aCTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGAtatt                                                                                                         <  2:512944/148‑1 (MQ=255)
                                         gcgcAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATCTTATgg                                                                                                     >  2:625525/1‑148 (MQ=255)
                                         gcgcAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATgg                                                                                                     >  1:423807/1‑148 (MQ=255)
                                           gcAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGaa                                                                                                   <  2:3415924/148‑1 (MQ=255)
                                            cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCACGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAg                                                                                                  <  1:3183803/148‑1 (MQ=255)
                                            cAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAg                                                                                                  <  1:691396/148‑1 (MQ=255)
                                             aGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGc                                                                                                 <  1:1599835/148‑1 (MQ=255)
                                                cGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGa                                                                                              <  2:2460138/148‑1 (MQ=255)
                                                cGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGa                                                                                              <  1:3097145/148‑1 (MQ=255)
                                                 taataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGaa                                                                                             <  2:2670074/147‑1 (MQ=255)
                                                  aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAac                                                                                            >  1:3400714/1‑148 (MQ=255)
                                                  aataatGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAac                                                                                            <  2:2363767/148‑1 (MQ=255)
                                                       tGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAcgcg                                                                                       >  2:2707053/1‑148 (MQ=255)
                                                            tGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                  >  1:1078646/1‑148 (MQ=255)
                                                            tGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAt                                                                                  >  1:1306522/1‑148 (MQ=255)
                                                              cAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATgg                                                                                <  1:2951538/148‑1 (MQ=255)
                                                              cAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATgg                                                                                <  2:3694492/148‑1 (MQ=255)
                                                                      gCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGTATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAAt                                                                        <  2:1083116/148‑1 (MQ=255)
                                                                          ggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCa                                                                    >  2:2084136/1‑148 (MQ=255)
                                                                          ggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCa                                                                    <  1:178713/148‑1 (MQ=255)
                                                                           gAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCaa                                                                   >  1:2781780/1‑148 (MQ=255)
                                                                           gAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCaa                                                                   >  2:305974/1‑148 (MQ=255)
                                                                            aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                  >  1:3685466/1‑148 (MQ=255)
                                                                            aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                  >  1:1256437/1‑148 (MQ=255)
                                                                            aaGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAat                                                                  >  1:2655283/1‑148 (MQ=255)
                                                                             aGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAata                                                                 >  1:1723293/1‑148 (MQ=255)
                                                                             aGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAata                                                                 >  1:1722694/1‑148 (MQ=255)
                                                                             aGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAata                                                                 <  2:1913166/148‑1 (MQ=255)
                                                                              gCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAatat                                                                >  1:2476047/1‑148 (MQ=255)
                                                                              gCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAatat                                                                <  2:187916/148‑1 (MQ=255)
                                                                               cTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAtt                                                               <  1:766654/148‑1 (MQ=255)
                                                                                tACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTGAATGGCGGCTAATGCCAATATTg                                                              <  2:3059895/148‑1 (MQ=255)
                                                                                   ggCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGgcg                                                           <  1:744541/148‑1 (MQ=255)
                                                                                      aaGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTg                                                        >  1:3293656/1‑148 (MQ=255)
                                                                                        gCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                      <  2:2612647/148‑1 (MQ=255)
                                                                                        gCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCa                                                      >  1:532331/1‑148 (MQ=255)
                                                                                           gCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCAcgc                                                   >  1:3669346/1‑148 (MQ=255)
                                                                                            ccGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCAcgcg                                                  >  2:969220/1‑148 (MQ=255)
                                                                                                  gcgcAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAt                                            <  1:2454467/148‑1 (MQ=255)
                                                                                                     cAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCAtttt                                         <  2:3611181/148‑1 (MQ=255)
                                                                                                        aCAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTc                                      >  1:2112506/1‑148 (MQ=255)
                                                                                                        aCAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTc                                      >  1:2140879/1‑148 (MQ=255)
                                                                                                           gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGt                                   <  1:1598615/148‑1 (MQ=255)
                                                                                                           gTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGt                                   <  2:1021883/148‑1 (MQ=255)
                                                                                                            tAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTc                                  >  2:567465/1‑148 (MQ=255)
                                                                                                            tAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTc                                  >  1:936689/1‑148 (MQ=255)
                                                                                                            tAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATATTTTCCGTc                                  <  2:1258671/148‑1 (MQ=255)
                                                                                                               aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                               >  2:1498574/1‑148 (MQ=255)
                                                                                                               aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                               <  1:2366265/148‑1 (MQ=255)
                                                                                                               aCAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtat                               >  2:1077503/1‑148 (MQ=255)
                                                                                                                cAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAGTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCtata                              <  2:2274292/148‑1 (MQ=255)
                                                                                                                 aGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATaa                             <  2:812265/148‑1 (MQ=255)
                                                                                                                    gACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGcc                          <  1:1154119/148‑1 (MQ=255)
                                                                                                                      cAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTg                        <  1:269741/148‑1 (MQ=255)
                                                                                                                      cAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTg                        <  1:3789595/148‑1 (MQ=255)
                                                                                                                        gCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAc                      >  1:2626027/1‑148 (MQ=255)
                                                                                                                           tGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAg                   <  2:506448/148‑1 (MQ=255)
                                                                                                                            gCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGc                  >  2:889583/1‑148 (MQ=255)
                                                                                                                              aaaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCgg                >  1:614455/1‑148 (MQ=255)
                                                                                                                               aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTTATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa               <  1:2339476/148‑1 (MQ=255)
                                                                                                                               aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa               <  1:2985462/148‑1 (MQ=255)
                                                                                                                               aaGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGa               <  1:2001503/148‑1 (MQ=255)
                                                                                                                                 gCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAat             >  1:1033373/1‑148 (MQ=255)
                                                                                                                                  cGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAata            >  1:680918/1‑148 (MQ=255)
                                                                                                                                  cGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAata            <  1:1399476/148‑1 (MQ=255)
                                                                                                                                   gTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAatat           <  1:1282576/148‑1 (MQ=255)
                                                                                                                                   gTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAatat           <  2:3489285/148‑1 (MQ=255)
                                                                                                                                     tAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCg         >  2:2625414/1‑148 (MQ=255)
                                                                                                                                      aaaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGa        <  1:2092399/148‑1 (MQ=255)
                                                                                                                                      aaaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGa        <  1:3574945/148‑1 (MQ=255)
                                                                                                                                       aaTTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGAc       <  2:3540789/148‑1 (MQ=255)
                                                                                                                                           aCCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGcc   <  2:1824824/148‑1 (MQ=255)
                                                                                                                                           aCCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGcc   <  2:1124317/148‑1 (MQ=255)
                                                                                                                                            ccGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCa  >  2:2355748/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCA  >  CP009273/591720‑592007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAG  >  CP009273/591716‑592011
                                                                                                                                                    |                                                                                                                                                   
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                    >  SRR19973069.1029036/1‑150 (MQ=60)
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                    <  SRR19973069.3806945/150‑1 (MQ=60)
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                    >  SRR19973069.367893/1‑150 (MQ=60)
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                    >  SRR19973069.1028954/1‑150 (MQ=60)
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAG                                                                                                                                                    >  SRR19973069.2307041/1‑150 (MQ=60)
  AGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGG                                                                                                                                                  >  SRR19973069.2259502/1‑150 (MQ=60)
    AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAT                                                                                                                                                >  SRR19973069.3660984/1‑150 (MQ=60)
    AAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGAT                                                                                                                                                >  SRR19973069.3660983/1‑150 (MQ=60)
     agggACCCGAAGCGAAATCTCTAAACGTCAGGGGGAACTGGCGCTGGAGAATAATGCTTTGCAACTGTTGCTGGGAAGCTAGGGAAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                               <  SRR19973069.1425427/146‑1 (MQ=60)
     AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                               <  SRR19973069.3758634/150‑1 (MQ=60)
     AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                               >  SRR19973069.2140789/1‑150 (MQ=60)
     AAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATT                                                                                                                                               <  SRR19973069.1608373/150‑1 (MQ=60)
      AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                              >  SRR19973069.189462/1‑150 (MQ=60)
      AGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTG                                                                                                                                              >  SRR19973069.356258/1‑150 (MQ=60)
       GTACCCGCAGCTACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGTAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGT                                                                                                                                             <  SRR19973069.1689004/150‑1 (MQ=60)
        TCCCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTC                                                                                                                                            <  SRR19973069.22920/150‑1 (MQ=60)
            CGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCG                                                                                                                                        >  SRR19973069.3776896/1‑150 (MQ=60)
              CAGCTACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATACTGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCA                                                                                                                                      <  SRR19973069.2619159/150‑1 (MQ=60)
              CAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCA                                                                                                                                      <  SRR19973069.2618523/150‑1 (MQ=60)
              CAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCA                                                                                                                                      <  SRR19973069.2927763/150‑1 (MQ=60)
               AGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAA                                                                                                                                     >  SRR19973069.3348793/1‑150 (MQ=60)
                   ACATCGCTAAGCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCT                                                                                                                                 >  SRR19973069.2121277/1‑150 (MQ=60)
                   ACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCT                                                                                                                                 >  SRR19973069.989949/1‑150 (MQ=60)
                   ACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCT                                                                                                                                 <  SRR19973069.590547/150‑1 (MQ=60)
                    CATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTT                                                                                                                                >  SRR19973069.3190060/1‑150 (MQ=60)
                     ATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTA                                                                                                                               >  SRR19973069.1276905/1‑150 (MQ=60)
                      TCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAT                                                                                                                              <  SRR19973069.812124/150‑1 (MQ=60)
                      TCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAT                                                                                                                              <  SRR19973069.810869/150‑1 (MQ=60)
                      TCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTAT                                                                                                                              <  SRR19973069.2859933/150‑1 (MQ=60)
                       CGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATT                                                                                                                             >  SRR19973069.461218/1‑150 (MQ=60)
                        GCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTG                                                                                                                            >  SRR19973069.2194391/1‑150 (MQ=60)
                          TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                          >  SRR19973069.449263/1‑150 (MQ=60)
                          TAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCA                                                                                                                          >  SRR19973069.921065/1‑150 (MQ=60)
                           AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                         >  SRR19973069.1123908/1‑150 (MQ=60)
                           AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                         >  SRR19973069.3827717/1‑150 (MQ=60)
                           AAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAG                                                                                                                         >  SRR19973069.1926357/1‑150 (MQ=60)
                            AACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGC                                                                                                                        >  SRR19973069.2795117/1‑150 (MQ=60)
                            AACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGC                                                                                                                        >  SRR19973069.3319992/1‑150 (MQ=60)
                             ACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATGGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCG                                                                                                                       <  SRR19973069.3532297/150‑1 (MQ=60)
                               GTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCC                                                                                                                     >  SRR19973069.2305389/1‑150 (MQ=60)
                               GTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCC                                                                                                                     >  SRR19973069.365754/1‑150 (MQ=60)
                               GTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGAAGCGCC                                                                                                                     >  SRR19973069.365498/1‑150 (MQ=60)
                                      GGAACTGGCGCAGGCTAATAATGATTTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACATTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATAT                                                                                                              <  SRR19973069.3256485/150‑1 (MQ=60)
                                      GGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATAT                                                                                                              <  SRR19973069.3256575/150‑1 (MQ=60)
                                       GAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATT                                                                                                             <  SRR19973069.866623/150‑1 (MQ=60)
                                           TGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGG                                                                                                         >  SRR19973069.426917/1‑150 (MQ=60)
                                             GCGCAGGCGAATAATGCATTGCAACTGTTGCTGGTAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAA                                                                                                       <  SRR19973069.1966803/150‑1 (MQ=60)
                                                CAGGCTAATAATGCATTGCAACTGTTGCTGGGAAGCTACGCCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                    <  SRR19973069.696142/150‑1 (MQ=60)
                                                CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCACGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                    <  SRR19973069.3204500/150‑1 (MQ=60)
                                                CAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCT                                                                                                    <  SRR19973069.696247/150‑1 (MQ=60)
                                                 AGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTG                                                                                                   >  SRR19973069.2976883/1‑150 (MQ=60)
                                                 AGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTG                                                                                                   <  SRR19973069.1610498/150‑1 (MQ=60)
                                                 AGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTG                                                                                                   <  SRR19973069.1610622/150‑1 (MQ=60)
                                                    CGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAAC                                                                                                <  SRR19973069.3117554/150‑1 (MQ=60)
                                                    CGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAAC                                                                                                >  SRR19973069.3422898/1‑150 (MQ=60)
                                                      AATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACAC                                                                                              >  SRR19973069.458771/1‑150 (MQ=60)
                                                       ATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACG                                                                                             >  SRR19973069.2075371/1‑150 (MQ=60)
                                                              ATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAT                                                                                      >  SRR19973069.1086168/1‑150 (MQ=60)
                                                              ATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAT                                                                                      >  SRR19973069.1315432/1‑150 (MQ=60)
                                                              ATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAT                                                                                      >  SRR19973069.1316163/1‑150 (MQ=60)
                                                              ATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAAT                                                                                      >  SRR19973069.1315397/1‑150 (MQ=60)
                                                                TGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGG                                                                                    >  SRR19973069.33691/1‑150 (MQ=60)
                                                                  CAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCG                                                                                  <  SRR19973069.2971123/150‑1 (MQ=60)
                                                                   AACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGG                                                                                 >  SRR19973069.510063/1‑150 (MQ=60)
                                                                       GTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAA                                                                             >  SRR19973069.3228246/1‑150 (MQ=60)
                                                                             GGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAA                                                                       >  SRR19973069.2800302/1‑150 (MQ=60)
                                                                             GGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAA                                                                       >  SRR19973069.3812139/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.3708205/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.1264924/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      <  SRR19973069.611420/150‑1 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.2673032/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      <  SRR19973069.610043/150‑1 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.2673079/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.3709547/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      >  SRR19973069.3709570/1‑150 (MQ=60)
                                                                              GGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAAT                                                                      <  SRR19973069.180213/150‑1 (MQ=60)
                                                                               GAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATA                                                                     >  SRR19973069.1735372/1‑150 (MQ=60)
                                                                               GAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATA                                                                     >  SRR19973069.1735292/1‑150 (MQ=60)
                                                                               GAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATA                                                                     >  SRR19973069.1734767/1‑150 (MQ=60)
                                                                                AAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                    >  SRR19973069.2492640/1‑150 (MQ=60)
                                                                                AAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                    >  SRR19973069.2492642/1‑150 (MQ=60)
                                                                                AAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                    <  SRR19973069.2181204/150‑1 (MQ=60)
                                                                                AAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                    <  SRR19973069.3016153/150‑1 (MQ=60)
                                                                                AAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATAT                                                                    >  SRR19973069.2492650/1‑150 (MQ=60)
                                                                                  GCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTG                                                                  <  SRR19973069.714481/150‑1 (MQ=60)
                                                                                   CTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGG                                                                 <  SRR19973069.428888/150‑1 (MQ=60)
                                                                                   CTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGG                                                                 <  SRR19973069.772065/150‑1 (MQ=60)
                                                                                   ATACGGCAAGCTGCCGAAAGCGAAGACTTTAAACAGCGACAGCCTTCAAAGCTTTAAATTACCGGTGGGATTGTCGTCGAAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGG                                                                 <  SRR19973069.770975/150‑1 (MQ=60)
                                                                                       GGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCT                                                             <  SRR19973069.749814/150‑1 (MQ=60)
                                                                                        GCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTG                                                            >  SRR19973069.3315224/1‑150 (MQ=60)
                                                                                          AAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCA                                                          >  SRR19973069.536083/1‑150 (MQ=60)
                                                                                             CTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGC                                                       >  SRR19973069.362789/1‑150 (MQ=60)
                                                                                             CTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGC                                                       >  SRR19973069.1792131/1‑150 (MQ=60)
                                                                                             CTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGC                                                       >  SRR19973069.3693361/1‑150 (MQ=60)
                                                                                              TGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCG                                                      <  SRR19973069.921843/150‑1 (MQ=60)
                                                                                              TGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCG                                                      <  SRR19973069.2846120/150‑1 (MQ=60)
                                                                                                CCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCG                                                    <  SRR19973069.3598039/150‑1 (MQ=60)
                                                                                                 CGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGG                                                   >  SRR19973069.2689687/1‑150 (MQ=60)
                                                                                                      GCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTT                                              <  SRR19973069.2470968/150‑1 (MQ=60)
                                                                                                          AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                          >  SRR19973069.2947618/1‑150 (MQ=60)
                                                                                                          AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                          >  SRR19973069.2155583/1‑150 (MQ=60)
                                                                                                          AGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTC                                          >  SRR19973069.2127069/1‑150 (MQ=60)
                                                                                                              AGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTC                                      >  SRR19973069.943031/1‑150 (MQ=60)
                                                                                                               GTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCT                                     <  SRR19973069.1609399/150‑1 (MQ=60)
                                                                                                                   ACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAA                                 <  SRR19973069.1244726/150‑1 (MQ=60)
                                                                                                                   ACAGCGACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAA                                 <  SRR19973069.2382335/150‑1 (MQ=60)
                                                                                                                        GACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTG                            <  SRR19973069.585083/150‑1 (MQ=60)
                                                                                                                        GACAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTG                            <  SRR19973069.1162166/150‑1 (MQ=60)
                                                                                                                          CAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAC                          >  SRR19973069.1698872/1‑150 (MQ=60)
                                                                                                                          CAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAC                          <  SRR19973069.3814171/150‑1 (MQ=60)
                                                                                                                          CAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAC                          >  SRR19973069.2643601/1‑150 (MQ=60)
                                                                                                                          CAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAC                          <  SRR19973069.271655/150‑1 (MQ=60)
                                                                                                                          CAGCCTGCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGAC                          >  SRR19973069.2643609/1‑150 (MQ=60)
                                                                                                                                GCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGG                    >  SRR19973069.618560/1‑150 (MQ=60)
                                                                                                                                GCAAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGG                    >  SRR19973069.619081/1‑150 (MQ=60)
                                                                                                                                  AAAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAA                  >  SRR19973069.880941/1‑150 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTTATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 <  SRR19973069.2355391/150‑1 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 >  SRR19973069.1040538/1‑150 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 >  SRR19973069.1043480/1‑150 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 <  SRR19973069.2015443/150‑1 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 <  SRR19973069.2355394/150‑1 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 <  SRR19973069.3263909/150‑1 (MQ=60)
                                                                                                                                   AAGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAAT                 <  SRR19973069.3005289/150‑1 (MQ=60)
                                                                                                                                    AGCGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATA                >  SRR19973069.685652/1‑150 (MQ=60)
                                                                                                                                      CGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATC              >  SRR19973069.2319566/1‑150 (MQ=60)
                                                                                                                                      CGTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATC              <  SRR19973069.1409242/150‑1 (MQ=60)
                                                                                                                                       GTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCG             <  SRR19973069.1291163/150‑1 (MQ=60)
                                                                                                                                       GTTAAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCG             <  SRR19973069.2871267/150‑1 (MQ=60)
                                                                                                                                          AAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACC          <  SRR19973069.2106866/150‑1 (MQ=60)
                                                                                                                                          AAATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACC          <  SRR19973069.3598246/150‑1 (MQ=60)
                                                                                                                                           AATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCG         >  SRR19973069.827460/1‑150 (MQ=60)
                                                                                                                                            ATTACCGGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGC        >  SRR19973069.2402342/1‑150 (MQ=60)
                                                                                                                                                  GGAGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAG  >  SRR19973069.2067106/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATTGCAGCGCCCGGATATTATGGAAGCTGAACACGCGTTAATGGCGGCTAATGCCAATATTGGCGCTGCACGCGCGGCATTTTTTCCGTCTATAAGCCTGACCAGCGGAATATCGACCGCCAGTAG  >  CP009273/591716‑592011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑