Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 624,669 G→T 93.5% T221T (ACG→ACT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273624,6690GT93.5% 480.4 / 24.7 168T221T (ACG→ACT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (8/3);  new base T (80/77);  total (88/80)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 168 total aligned reads displayed.

CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAA  >  CP009273/624524‑624813
                                                                                                                                                 |                                                                                                                                                
 gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtt                                                                                                                                               <  2:2666606/148‑1 (MQ=255)
  ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                              <  1:125804/148‑1 (MQ=255)
  ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                              <  1:2123090/148‑1 (MQ=255)
  ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                              <  1:1799142/148‑1 (MQ=255)
    tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                            <  2:3008694/148‑1 (MQ=255)
    tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                            <  1:450356/148‑1 (MQ=255)
     ggCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGt                                                                                                                                           >  1:772376/1‑148 (MQ=255)
       cTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTc                                                                                                                                         <  2:2310501/148‑1 (MQ=255)
         ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCt                                                                                                                                       >  2:1626547/1‑148 (MQ=255)
           accgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                     >  1:907171/1‑148 (MQ=255)
           accgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                     >  1:907188/1‑148 (MQ=255)
            ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGc                                                                                                                                    >  2:1487699/1‑148 (MQ=255)
            ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGc                                                                                                                                    >  2:1488080/1‑148 (MQ=255)
            ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGc                                                                                                                                    <  2:1335862/148‑1 (MQ=255)
              gacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCct                                                                                                                                  <  1:2478586/148‑1 (MQ=255)
               acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctc                                                                                                                                 >  1:1773187/1‑148 (MQ=255)
               acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctc                                                                                                                                 <  1:1917370/148‑1 (MQ=255)
               acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCAGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTCTTTTGTTCCTCGCctc                                                                                                                                 >  2:3062184/1‑148 (MQ=255)
                 accgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTg                                                                                                                               >  1:2733133/1‑148 (MQ=255)
                       atGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCg                                                                                                                         >  1:2138246/1‑148 (MQ=255)
                        tGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgc                                                                                                                        <  2:702942/148‑1 (MQ=255)
                        tGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCgc                                                                                                                        <  2:702776/148‑1 (MQ=255)
                            acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagc                                                                                                                    >  2:500233/1‑148 (MQ=255)
                            acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagc                                                                                                                    >  2:524414/1‑148 (MQ=255)
                               cacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                 <  2:2039679/148‑1 (MQ=255)
                               cacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                 >  1:394355/1‑148 (MQ=255)
                               cacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                 >  1:394251/1‑148 (MQ=255)
                                  gcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATAtt                                                                                                              <  1:2457146/148‑1 (MQ=255)
                                  gcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATAtt                                                                                                              <  1:2457150/148‑1 (MQ=255)
                                   cTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTa                                                                                                             <  2:2853559/148‑1 (MQ=255)
                                   cTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTa                                                                                                             >  1:1882764/1‑148 (MQ=255)
                                    tgtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAc                                                                                                            <  1:132993/148‑1 (MQ=255)
                                          tGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACa                                                                                                      <  1:1748915/148‑1 (MQ=255)
                                            aGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAgg                                                                                                    >  2:1761362/1‑148 (MQ=255)
                                             gCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGa                                                                                                   <  1:1465428/148‑1 (MQ=255)
                                              cGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                  <  2:3235795/148‑1 (MQ=255)
                                               gATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGata                                                                                                 <  2:165187/148‑1 (MQ=255)
                                                aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGatat                                                                                                <  1:1274570/148‑1 (MQ=255)
                                                 tGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                               >  1:2896794/1‑148 (MQ=255)
                                                 tGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                               <  1:2278733/148‑1 (MQ=255)
                                                  gACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATGTtg                                                                                              <  1:1387572/148‑1 (MQ=255)
                                                   aCGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATtgt                                                                                             <  2:1772757/148‑1 (MQ=255)
                                                   aCGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATtgt                                                                                             <  2:518814/148‑1 (MQ=255)
                                                        gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCg                                                                                        <  1:637852/148‑1 (MQ=255)
                                                        gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCg                                                                                        >  1:2290492/1‑148 (MQ=255)
                                                         aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                       >  1:264741/1‑148 (MQ=255)
                                                         aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                       >  1:570301/1‑148 (MQ=255)
                                                         aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                       <  2:1001521/148‑1 (MQ=255)
                                                           gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTCCAGGATATTGTGGTCGATg                                                                                     >  2:2554452/1‑148 (MQ=255)
                                                           gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATg                                                                                     >  2:1432351/1‑148 (MQ=255)
                                                            aaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAATCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                    >  2:2432588/1‑148 (MQ=255)
                                                            aaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATCTTGTGGTCGATgg                                                                                    >  2:732550/1‑148 (MQ=255)
                                                                 cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCt                                                                               <  2:2853393/148‑1 (MQ=255)
                                                                 cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACATCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCt                                                                               >  2:1893566/1‑148 (MQ=255)
                                                                   tATTCGCTGCTTTGGCGAGCAGTTTAAACTCTGCATTCCGCTGGGGAAAATCGCCCTTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCa                                                                             <  2:40470/148‑1 (MQ=255)
                                                                      tCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                          <  2:1046850/148‑1 (MQ=255)
                                                                       cgcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGc                                                                         <  1:290566/148‑1 (MQ=255)
                                                                          ggCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgg                                                                      <  2:2113909/148‑1 (MQ=255)
                                                                              ttGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGc                                                                  <  1:620494/148‑1 (MQ=255)
                                                                                 gCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATa                                                               <  1:2717222/148‑1 (MQ=255)
                                                                                    aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                            <  2:626089/148‑1 (MQ=255)
                                                                                     gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                           >  2:1116794/1‑148 (MQ=255)
                                                                                     gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                           <  1:1626547/148‑1 (MQ=255)
                                                                                     gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                           <  2:359042/148‑1 (MQ=255)
                                                                                      cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                          >  1:2333314/1‑148 (MQ=255)
                                                                                      cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGAGATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                          >  1:1040719/1‑148 (MQ=255)
                                                                                         tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAt                                                       >  1:3275300/1‑148 (MQ=255)
                                                                                               cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                 >  1:2999921/1‑148 (MQ=255)
                                                                                                tCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                >  1:1070297/1‑148 (MQ=255)
                                                                                                tCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                <  1:2163136/148‑1 (MQ=255)
                                                                                                   gCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGc                                             <  2:1658400/148‑1 (MQ=255)
                                                                                                   gCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGc                                             >  2:3190131/1‑148 (MQ=255)
                                                                                                    ctttCGGCTGGGTAAAATCGCCCGTCCACAAGAGATCGCAAACACTATTTTGTTCCTCGCCTCTTACCTCTCCAGCCATATTACCCTACAGTATATTGTGGTCGTTGGCGGATCAACGCTGGGGGCATAAGCATGATCTGGAAACGcc                                            <  1:2787171/146‑1 (MQ=255)
                                                                                                     aTTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCa                                           <  1:1645236/148‑1 (MQ=255)
                                                                                                       tCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAtt                                         >  1:2666665/1‑148 (MQ=255)
                                                                                                              gggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                  >  2:1861363/1‑148 (MQ=255)
                                                                                                              gggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                  <  1:795731/148‑1 (MQ=255)
                                                                                                                gAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcg                                >  2:1292449/1‑148 (MQ=255)
                                                                                                                    aTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATCTTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgacga                            >  2:1728657/1‑148 (MQ=255)
                                                                                                                        cccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                        <  1:2751555/148‑1 (MQ=255)
                                                                                                                        cccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                        >  1:2495475/1‑148 (MQ=255)
                                                                                                                        cccGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                        >  1:1018937/1‑148 (MQ=255)
                                                                                                                         ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGa                       >  2:2947267/1‑148 (MQ=255)
                                                                                                                          cGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGaa                      >  2:838463/1‑148 (MQ=255)
                                                                                                                             ccACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGc                   >  2:1071593/1‑148 (MQ=255)
                                                                                                                                caAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCCGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac                >  1:1766335/1‑148 (MQ=255)
                                                                                                                                 aaGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGAATAAGCATTATCTGTAAACGCCATTTAACGCTCGACGAACTGAACGccacc               <  1:732550/148‑1 (MQ=255)
                                                                                                                                    agaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGc            >  1:965492/1‑148 (MQ=255)
                                                                                                                                     gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg           >  2:1320614/1‑148 (MQ=255)
                                                                                                                                     gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg           >  2:547733/1‑148 (MQ=255)
                                                                                                                                      aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGa          >  1:1497589/1‑148 (MQ=255)
                                                                                                                                      aTCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGa          >  1:3199774/1‑148 (MQ=255)
                                                                                                                                      aTCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGa          >  1:3199818/1‑148 (MQ=255)
                                                                                                                                          ccAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAac      <  1:2048697/148‑1 (MQ=255)
                                                                                                                                             acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca   <  2:3182077/148‑1 (MQ=255)
                                                                                                                                             acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca   <  1:500305/148‑1 (MQ=255)
                                                                                                                                              cacTATTTTGTTCCTCGCCTCTGACCTCGTCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa  <  2:3303044/148‑1 (MQ=255)
                                                                                                                                              cacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa  >  2:1817845/1‑148 (MQ=255)
                                                                                                                                              cacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa  <  2:394251/148‑1 (MQ=255)
                                                                                                                                              cacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa  >  2:1328764/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAA  >  CP009273/624524‑624813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  >  CP009273/624520‑624818
                                                                                                                                                     |                                                                                                                                                     
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACT                                                                                                                                                       >  SRR19973070.3239318/1‑150 (MQ=60)
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACT                                                                                                                                                       <  SRR19973070.2027111/150‑1 (MQ=60)
  TTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAT                                                                                                                                                     <  SRR19973070.227148/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                    <  SRR19973070.2397817/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                    <  SRR19973070.386309/150‑1 (MQ=60)
    CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTT                                                                                                                                                   <  SRR19973070.973190/150‑1 (MQ=60)
    CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTT                                                                                                                                                   <  SRR19973070.2371515/150‑1 (MQ=60)
    CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTT                                                                                                                                                   <  SRR19973070.2246289/150‑1 (MQ=60)
      CCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTG                                                                                                                                                 <  SRR19973070.126910/150‑1 (MQ=60)
      CCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTG                                                                                                                                                 <  SRR19973070.2137300/150‑1 (MQ=60)
      CCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTG                                                                                                                                                 <  SRR19973070.1811564/150‑1 (MQ=60)
       CTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGT                                                                                                                                                >  SRR19973070.777790/1‑150 (MQ=60)
        TGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTT                                                                                                                                               <  SRR19973070.3238560/150‑1 (MQ=60)
        TGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTT                                                                                                                                               >  SRR19973070.630515/1‑150 (MQ=60)
        TGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTT                                                                                                                                               <  SRR19973070.453601/150‑1 (MQ=60)
         GGCTCCACCGACACCGATATGTAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTC                                                                                                                                              >  SRR19973070.3324196/1‑150 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                          >  SRR19973070.913629/1‑150 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                          >  SRR19973070.913612/1‑150 (MQ=60)
                 CGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTC                                                                                                                                      >  SRR19973070.2089763/1‑150 (MQ=60)
                 CGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTC                                                                                                                                      >  SRR19973070.1785462/1‑150 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCT                                                                                                                                     <  SRR19973070.2494726/150‑1 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCT                                                                                                                                     >  SRR19973070.794567/1‑150 (MQ=60)
                   ACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTG                                                                                                                                    >  SRR19973070.2750749/1‑150 (MQ=60)
                   ACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTG                                                                                                                                    <  SRR19973070.1930304/150‑1 (MQ=60)
                         ATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCG                                                                                                                              >  SRR19973070.2152512/1‑150 (MQ=60)
                                 CGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCAT                                                                                                                      >  SRR19973070.397255/1‑150 (MQ=60)
                                 CGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCAT                                                                                                                      >  SRR19973070.397147/1‑150 (MQ=60)
                                     CGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTA                                                                                                                  >  SRR19973070.1895560/1‑150 (MQ=60)
                                      GCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAC                                                                                                                 <  SRR19973070.2473206/150‑1 (MQ=60)
                                      GCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAC                                                                                                                 <  SRR19973070.2473202/150‑1 (MQ=60)
                                      GCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTACTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAC                                                                                                                 <  SRR19973070.2473203/150‑1 (MQ=60)
                                       CTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACC                                                                                                                >  SRR19973070.920209/1‑150 (MQ=60)
                                        TGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCC                                                                                                               <  SRR19973070.134151/150‑1 (MQ=60)
                                              TGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGG                                                                                                         <  SRR19973070.1761036/150‑1 (MQ=60)
                                              TGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGG                                                                                                         <  SRR19973070.1761018/150‑1 (MQ=60)
                                                 GCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATA                                                                                                      <  SRR19973070.1475666/150‑1 (MQ=60)
                                                   GATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATT                                                                                                    >  SRR19973070.2915532/1‑150 (MQ=60)
                                                    ATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTG                                                                                                   <  SRR19973070.1283483/150‑1 (MQ=60)
                                                    ATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTG                                                                                                   <  SRR19973070.1479406/150‑1 (MQ=60)
                                                     TGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGT                                                                                                  <  SRR19973070.2293871/150‑1 (MQ=60)
                                                      GACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATGTTGTG                                                                                                 <  SRR19973070.1396952/150‑1 (MQ=60)
                                                      GACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTG                                                                                                 <  SRR19973070.1396951/150‑1 (MQ=60)
                                                          CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCG                                                                                             >  SRR19973070.2305719/1‑150 (MQ=60)
                                                          CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCG                                                                                             >  SRR19973070.2305745/1‑150 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGA                                                                                            >  SRR19973070.574166/1‑150 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGA                                                                                            >  SRR19973070.266498/1‑150 (MQ=60)
                                                            GAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAT                                                                                           <  SRR19973070.642393/150‑1 (MQ=60)
                                                              AGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGG                                                                                         >  SRR19973070.1791709/1‑150 (MQ=60)
                                                               GAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGC                                                                                        >  SRR19973070.1329662/1‑150 (MQ=60)
                                                                           CGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTG                                                                            >  SRR19973070.43594/1‑150 (MQ=60)
                                                                           CGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTG                                                                            <  SRR19973070.292544/150‑1 (MQ=60)
                                                                            GCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGG                                                                           <  SRR19973070.856895/150‑1 (MQ=60)
                                                                                CTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGC                                                                       >  SRR19973070.3202667/1‑150 (MQ=60)
                                                                                  TTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCAT                                                                     <  SRR19973070.624861/150‑1 (MQ=60)
                                                                                     GCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAG                                                                  <  SRR19973070.2734767/150‑1 (MQ=60)
                                                                                        AGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAT                                                               >  SRR19973070.2348808/1‑150 (MQ=60)
                                                                                        AGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGAGATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAT                                                               >  SRR19973070.1048067/1‑150 (MQ=60)
                                                                                         GCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATG                                                              <  SRR19973070.2966157/150‑1 (MQ=60)
                                                                                         GCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATG                                                              <  SRR19973070.1637841/150‑1 (MQ=60)
                                                                                          CAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGA                                                             >  SRR19973070.285410/1‑150 (MQ=60)
                                                                                          CAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGA                                                             <  SRR19973070.1120191/150‑1 (MQ=60)
                                                                                           AGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAT                                                            >  SRR19973070.3296350/1‑150 (MQ=60)
                                                                                           AGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAA                                                            >  SRR19973070.2504968/1‑150 (MQ=60)
                                                                                                 AACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAA                                                      >  SRR19973070.3019115/1‑150 (MQ=60)
                                                                                                 AACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAA                                                      >  SRR19973070.2577265/1‑150 (MQ=60)
                                                                                                  ACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAA                                                     >  SRR19973070.1077783/1‑150 (MQ=60)
                                                                                                    TCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACG                                                   >  SRR19973070.60935/1‑150 (MQ=60)
                                                                                                    TCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACG                                                   <  SRR19973070.2177483/150‑1 (MQ=60)
                                                                                                    TCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACG                                                   <  SRR19973070.2177504/150‑1 (MQ=60)
                                                                                                      GGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCC                                                 >  SRR19973070.1777219/1‑150 (MQ=60)
                                                                                                        CTTTCGGCTGGGTAAAATCGCCCGTCCACAAGAGATCGCAAACACTATTTTGTTCCTCGCCTCTTACCTCTCCAGCCATATTACCCTACAGTATATTGTGGTCGTTGGCGGATCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAT                                               <  SRR19973070.2805112/150‑1 (MQ=60)
                                                                                                        CATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAT                                               <  SRR19973070.2802847/150‑1 (MQ=60)
                                                                                                         ATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATT                                              >  SRR19973070.2683986/1‑150 (MQ=60)
                                                                                                         ATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATT                                              >  SRR19973070.2684026/1‑150 (MQ=60)
                                                                                                         ATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATT                                              <  SRR19973070.1656605/150‑1 (MQ=60)
                                                                                                                TGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                       >  SRR19973070.1074221/1‑150 (MQ=60)
                                                                                                                  GGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCG                                     <  SRR19973070.801227/150‑1 (MQ=60)
                                                                                                                         TCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACT                              <  SRR19973070.738029/150‑1 (MQ=60)
                                                                                                                          CGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTG                             >  SRR19973070.2511659/1‑150 (MQ=60)
                                                                                                                          CGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTG                             >  SRR19973070.2023033/1‑150 (MQ=60)
                                                                                                                          CGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTG                             >  SRR19973070.1026175/1‑150 (MQ=60)
                                                                                                                            CCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAA                           <  SRR19973070.2769230/150‑1 (MQ=60)
                                                                                                                            CCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAA                           >  SRR19973070.1812007/1‑150 (MQ=60)
                                                                                                                                 CCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCA                      <  SRR19973070.2740520/150‑1 (MQ=60)
                                                                                                                                  CACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCCGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCAC                     >  SRR19973070.1778558/1‑150 (MQ=60)
                                                                                                                                     AAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGAATAAGCATTATCTGTAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAG                  <  SRR19973070.737739/150‑1 (MQ=60)
                                                                                                                                      AGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGC                 >  SRR19973070.972386/1‑150 (MQ=60)
                                                                                                                                        AGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA               >  SRR19973070.1508072/1‑150 (MQ=60)
                                                                                                                                        AGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA               >  SRR19973070.3220522/1‑150 (MQ=60)
                                                                                                                                        AGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA               >  SRR19973070.3220478/1‑150 (MQ=60)
                                                                                                                                        AGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA               >  SRR19973070.1571409/1‑150 (MQ=60)
                                                                                                                                              CCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACAC         <  SRR19973070.2062556/150‑1 (MQ=60)
                                                                                                                                                 ACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAT      <  SRR19973070.503711/150‑1 (MQ=60)
                                                                                                                                                 ACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAT      <  SRR19973070.503783/150‑1 (MQ=60)
                                                                                                                                                    CTATTTTGTTCCTCTCCTCTGACCTCGCCAGCAATTTGACCCTACTGGATATTGTTGTCGATGGCGGCTCAACGCTGGGGGAATAAGAATGATCTGGAAACGCCATTTAACGCTCGACTAACTGAACGCCACCAGCGATAACACAATGGT   <  SRR19973070.3081792/150‑1 (MQ=60)
                                                                                                                                                     TATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  <  SRR19973070.72203/150‑1 (MQ=60)
                                                                                                                                                     TATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  <  SRR19973070.2974489/150‑1 (MQ=60)
                                                                                                                                                     TATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  >  SRR19973070.2036864/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  >  CP009273/624520‑624818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑