Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 624,669 G→T 88.1% T221T (ACG→ACT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273624,6690GT88.1% 663.1 / 83.5 262T221T (ACG→ACT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/13);  new base T (104/126);  total (123/139)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624523‑624817
                                                                                                                                                  |                                                                                                                                                    
  gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                  <  2:3065718/149‑1 (MQ=255)
  gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                  >  2:3180673/1‑149 (MQ=255)
    cTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                                <  1:1365782/149‑1 (MQ=255)
     tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGt                                                                                                                                               >  1:3828436/1‑149 (MQ=255)
     tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGt                                                                                                                                               <  2:4463658/149‑1 (MQ=255)
      ggCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                              <  2:1710612/149‑1 (MQ=255)
      ggCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                              <  2:3996376/149‑1 (MQ=255)
       gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTc                                                                                                                                             >  1:558394/1‑149 (MQ=255)
       gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTc                                                                                                                                             <  2:4491805/149‑1 (MQ=255)
        cTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTcc                                                                                                                                            >  2:4106881/1‑149 (MQ=255)
         tCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCt                                                                                                                                           <  2:2608864/149‑1 (MQ=255)
          ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTc                                                                                                                                          <  1:2250264/149‑1 (MQ=255)
           caccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGACGACCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                         >  2:529040/1‑149 (MQ=255)
             ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGcc                                                                                                                                       <  1:2180948/149‑1 (MQ=255)
             ccgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGcc                                                                                                                                       <  2:2355198/149‑1 (MQ=255)
              cgacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCct                                                                                                                                      <  1:4540756/149‑1 (MQ=255)
                acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctct                                                                                                                                    <  1:2596854/149‑1 (MQ=255)
                acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCctct                                                                                                                                    >  1:1248256/1‑149 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCt                                                                                                                               <  1:3012480/149‑1 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCt                                                                                                                               <  1:3674247/149‑1 (MQ=255)
                     gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCt                                                                                                                               >  2:31451/1‑149 (MQ=255)
                         tGCAACGCACGCTGTGTGTGAGAGATGACGCCGAAGAACAGAGTATTCGCGGCTTTGGAGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTAGTTCCTCGCCTCTGACATCgcc                                                                                                                           >  2:4364929/1‑149 (MQ=255)
                         tGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgcc                                                                                                                           >  2:1937404/1‑149 (MQ=255)
                            aaCGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagc                                                                                                                        >  1:4680068/1‑149 (MQ=255)
                             acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                       >  1:44775/1‑149 (MQ=255)
                             acgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCACCAACACGATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                       <  2:1433417/149‑1 (MQ=255)
                               gcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                     >  1:3399791/1‑149 (MQ=255)
                               gcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                     >  2:4316192/1‑149 (MQ=255)
                                     tgtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAcc                                                                                                               <  2:4179020/149‑1 (MQ=255)
                                         ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTAc                                                                                                           <  2:3725637/149‑1 (MQ=255)
                                              gCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                      <  2:1281909/149‑1 (MQ=255)
                                                gATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGatat                                                                                                    >  1:1136216/1‑149 (MQ=255)
                                                 aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                                   >  2:2266478/1‑149 (MQ=255)
                                                 aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                                   >  2:4784342/1‑149 (MQ=255)
                                                     cGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTgg                                                                                               <  1:4170894/149‑1 (MQ=255)
                                                       ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTc                                                                                             <  1:3488920/149‑1 (MQ=255)
                                                         gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                           >  2:2910642/1‑149 (MQ=255)
                                                         gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                           <  2:1586395/149‑1 (MQ=255)
                                                          aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                          >  2:1962897/1‑149 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        <  1:4087356/149‑1 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        <  2:665254/149‑1 (MQ=255)
                                                            gaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                        >  2:1338843/1‑149 (MQ=255)
                                                                  cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                  <  1:39681/149‑1 (MQ=255)
                                                                  cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                  <  1:4312352/149‑1 (MQ=255)
                                                                   gTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCa                                                                                 >  1:1451831/1‑149 (MQ=255)
                                                                    tATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCaa                                                                                >  1:410408/1‑149 (MQ=255)
                                                                      ttCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                              >  2:1392810/1‑149 (MQ=255)
                                                                           ggCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggg                                                                         <  1:3290977/149‑1 (MQ=255)
                                                                            gCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgggg                                                                        >  1:2161781/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  2:4150535/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  2:277598/1‑149 (MQ=255)
                                                                             cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                       >  1:2338274/1‑149 (MQ=255)
                                                                                 ggCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATa                                                                   <  1:3323139/149‑1 (MQ=255)
                                                                                  gCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATaa                                                                  <  1:1558373/149‑1 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                >  1:644824/1‑149 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                <  1:2726731/149‑1 (MQ=255)
                                                                                    gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                                >  2:4287147/1‑149 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               <  2:3248070/149‑1 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               <  2:4671793/149‑1 (MQ=255)
                                                                                     aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                               >  1:734513/1‑149 (MQ=255)
                                                                                       cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATg                                                             <  2:3828436/149‑1 (MQ=255)
                                                                                       cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATg                                                             <  2:3611749/149‑1 (MQ=255)
                                                                                         gTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAt                                                           <  2:4547389/149‑1 (MQ=255)
                                                                                          tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATc                                                          <  2:515094/149‑1 (MQ=255)
                                                                                              aaCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGa                                                      >  1:3790234/1‑149 (MQ=255)
                                                                                               aCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                     >  1:3219322/1‑149 (MQ=255)
                                                                                               aCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                     <  2:4680068/149‑1 (MQ=255)
                                                                                                cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                    <  2:1660957/149‑1 (MQ=255)
                                                                                                cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaaa                                                    <  1:4535/149‑1 (MQ=255)
                                                                                                    gCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGcc                                                >  1:3629954/1‑149 (MQ=255)
                                                                                                         ccGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTa                                           >  2:2689080/1‑149 (MQ=255)
                                                                                                           gCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAc                                         >  1:1827136/1‑149 (MQ=255)
                                                                                                              ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                      >  2:1361683/1‑149 (MQ=255)
                                                                                                              ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                      >  1:815865/1‑149 (MQ=255)
                                                                                                                ggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGACACGCCATTTAACGCTcg                                    >  2:1015879/1‑149 (MQ=255)
                                                                                                                 gAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                                   >  2:1387012/1‑149 (MQ=255)
                                                                                                                  aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgac                                  <  2:709015/149‑1 (MQ=255)
                                                                                                                  aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgac                                  <  2:708001/149‑1 (MQ=255)
                                                                                                                     aTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGaa                               >  2:150489/1‑149 (MQ=255)
                                                                                                                       cGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACt                             >  1:2915199/1‑149 (MQ=255)
                                                                                                                        gCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                            <  2:3540897/149‑1 (MQ=255)
                                                                                                                        gCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                            <  1:1703702/149‑1 (MQ=255)
                                                                                                                         cccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGa                           >  1:2408284/1‑149 (MQ=255)
                                                                                                                           cGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                         >  1:1244469/1‑149 (MQ=255)
                                                                                                                           cGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCCGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                         >  1:3108830/1‑149 (MQ=255)
                                                                                                                             tCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGc                       <  2:2397677/149‑1 (MQ=255)
                                                                                                                               cacaAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGcca                     >  1:2825560/1‑149 (MQ=255)
                                                                                                                                acaAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac                    >  2:303036/1‑149 (MQ=255)
                                                                                                                                   agagATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg                 >  2:2892830/1‑149 (MQ=255)
                                                                                                                                   agagATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg                 >  1:88635/1‑149 (MQ=255)
                                                                                                                                    gagaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGc                >  1:1078551/1‑149 (MQ=255)
                                                                                                                                     agaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCTCCTGCCATATTACCCTACAGGATATTGTGGTCGATGGCTGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACTCTCGACGAACTGAACGCCACCAGCg               <  2:3534608/149‑1 (MQ=255)
                                                                                                                                       aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt             <  1:3424845/149‑1 (MQ=255)
                                                                                                                                       aTCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt             <  2:1814609/149‑1 (MQ=255)
                                                                                                                                        tCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATa            >  1:2094420/1‑149 (MQ=255)
                                                                                                                                           ccAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAaca         <  2:2183816/149‑1 (MQ=255)
                                                                                                                                            cAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAacac        <  1:3523184/149‑1 (MQ=255)
                                                                                                                                            cAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAacac        <  2:2140238/149‑1 (MQ=255)
                                                                                                                                             aaCACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca       <  2:867570/149‑1 (MQ=255)
                                                                                                                                              acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATCTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa      >  2:3703719/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624523‑624817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  >  CP009273/624520‑624818
                                                                                                                                                     |                                                                                                                                                     
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACT                                                                                                                                                       <  SRR19973071.2539437/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                    <  SRR19973071.1177304/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                    <  SRR19973071.1177575/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                    <  SRR19973071.1420/150‑1 (MQ=60)
      CCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTG                                                                                                                                                 >  SRR19973071.966129/1‑150 (MQ=60)
      CCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTG                                                                                                                                                 <  SRR19973071.208307/150‑1 (MQ=60)
       CTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGT                                                                                                                                                >  SRR19973071.3842008/1‑150 (MQ=60)
       CTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGT                                                                                                                                                <  SRR19973071.1370822/150‑1 (MQ=60)
        TGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTT                                                                                                                                               >  SRR19973071.3653763/1‑150 (MQ=60)
         GGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTC                                                                                                                                              >  SRR19973071.560495/1‑150 (MQ=60)
          GCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCC                                                                                                                                             <  SRR19973071.1657829/150‑1 (MQ=60)
           CTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCT                                                                                                                                            <  SRR19973071.2177093/150‑1 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                          <  SRR19973071.2258560/150‑1 (MQ=60)
                CCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCT                                                                                                                                       <  SRR19973071.2189012/150‑1 (MQ=60)
                 CGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTC                                                                                                                                      >  SRR19973071.2602533/1‑150 (MQ=60)
                 CGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTC                                                                                                                                      <  SRR19973071.4556932/150‑1 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCT                                                                                                                                     <  SRR19973071.4123680/150‑1 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCT                                                                                                                                     <  SRR19973071.3911024/150‑1 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCT                                                                                                                                     >  SRR19973071.1252639/1‑150 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCT                                                                                                                                     >  SRR19973071.1252722/1‑150 (MQ=60)
                   ACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTG                                                                                                                                    <  SRR19973071.2606253/150‑1 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCATTTTAAACTCGGCTTTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                   <  SRR19973071.3534843/150‑1 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                   >  SRR19973071.336771/1‑150 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                   <  SRR19973071.3534859/150‑1 (MQ=60)
                        GATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTC                                                                                                                               <  SRR19973071.3023228/150‑1 (MQ=60)
                        GATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTC                                                                                                                               <  SRR19973071.3687225/150‑1 (MQ=60)
                            TGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCA                                                                                                                           <  SRR19973071.296681/150‑1 (MQ=60)
                            TGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCA                                                                                                                           <  SRR19973071.2698988/150‑1 (MQ=60)
                             GCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAG                                                                                                                          <  SRR19973071.517094/150‑1 (MQ=60)
                              CAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGC                                                                                                                         >  SRR19973071.4696713/1‑150 (MQ=60)
                               AACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCC                                                                                                                        >  SRR19973071.45048/1‑150 (MQ=60)
                                 CGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCAT                                                                                                                      >  SRR19973071.3411838/1‑150 (MQ=60)
                                    ACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATT                                                                                                                   <  SRR19973071.4493396/150‑1 (MQ=60)
                                                AGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGAT                                                                                                       >  SRR19973071.4135365/1‑150 (MQ=60)
                                                  CGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAT                                                                                                     >  SRR19973071.1140189/1‑150 (MQ=60)
                                                    ATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTG                                                                                                   <  SRR19973071.963823/150‑1 (MQ=60)
                                                        CGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGT                                                                                               <  SRR19973071.4185747/150‑1 (MQ=60)
                                                          CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCG                                                                                             <  SRR19973071.3776790/150‑1 (MQ=60)
                                                          CCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCG                                                                                             <  SRR19973071.3501189/150‑1 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGCTATTGTGGTCGA                                                                                            >  SRR19973071.3427571/1‑150 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATCTTGTGGTCGA                                                                                            >  SRR19973071.3438454/1‑150 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGA                                                                                            >  SRR19973071.606338/1‑150 (MQ=60)
                                                           CGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGA                                                                                            >  SRR19973071.1635488/1‑150 (MQ=60)
                                                              AGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGG                                                                                         <  SRR19973071.1387918/150‑1 (MQ=60)
                                                              AGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGG                                                                                         <  SRR19973071.2837304/150‑1 (MQ=60)
                                                               GAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGC                                                                                        <  SRR19973071.4101870/150‑1 (MQ=60)
                                                                 ACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGG                                                                                      >  SRR19973071.3077083/1‑150 (MQ=60)
                                                                    GCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTC                                                                                   <  SRR19973071.2340394/150‑1 (MQ=60)
                                                                    GCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTC                                                                                   <  SRR19973071.2340357/150‑1 (MQ=60)
                                                                    GCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTC                                                                                   <  SRR19973071.756689/150‑1 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  >  SRR19973071.2940246/1‑150 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  >  SRR19973071.1457145/1‑150 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  <  SRR19973071.39929/150‑1 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  >  SRR19973071.2245668/1‑150 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  >  SRR19973071.1511887/1‑150 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCA                                                                                  <  SRR19973071.4327597/150‑1 (MQ=60)
                                                                      GTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAA                                                                                 >  SRR19973071.411744/1‑150 (MQ=60)
                                                                            GCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGG                                                                           <  SRR19973071.4015477/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         <  SRR19973071.3303403/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         <  SRR19973071.3302639/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         <  SRR19973071.1259448/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         <  SRR19973071.1259507/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         >  SRR19973071.2169774/1‑150 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                         >  SRR19973071.2169817/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                        >  SRR19973071.4752269/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                        >  SRR19973071.2346769/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                        <  SRR19973071.2862807/150‑1 (MQ=60)
                                                                                CTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGC                                                                       >  SRR19973071.4510676/1‑150 (MQ=60)
                                                                                CTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGC                                                                       >  SRR19973071.2103187/1‑150 (MQ=60)
                                                                                   TGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATA                                                                    <  SRR19973071.4481328/150‑1 (MQ=60)
                                                                                    GGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAA                                                                   <  SRR19973071.3263854/150‑1 (MQ=60)
                                                                                    GGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAA                                                                   <  SRR19973071.3334919/150‑1 (MQ=60)
                                                                                     GCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAG                                                                  <  SRR19973071.1563925/150‑1 (MQ=60)
                                                                                      CGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGC                                                                 >  SRR19973071.647182/1‑150 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                                <  SRR19973071.3195511/150‑1 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                                >  SRR19973071.737056/1‑150 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                                <  SRR19973071.2737127/150‑1 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                                <  SRR19973071.2736426/150‑1 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                                >  SRR19973071.3837598/1‑150 (MQ=60)
                                                                                          CAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGA                                                             <  SRR19973071.749326/150‑1 (MQ=60)
                                                                                           AGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAT                                                            >  SRR19973071.3355249/1‑150 (MQ=60)
                                                                                                AAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGA                                                       >  SRR19973071.4055083/1‑150 (MQ=60)
                                                                                                AAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGA                                                       >  SRR19973071.3803702/1‑150 (MQ=60)
                                                                                                 AACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAA                                                      >  SRR19973071.3200709/1‑150 (MQ=60)
                                                                                                 AACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAA                                                      >  SRR19973071.3230627/1‑150 (MQ=60)
                                                                                                   CTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAC                                                    <  SRR19973071.4576/150‑1 (MQ=60)
                                                                                                      GGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCC                                                 >  SRR19973071.3642701/1‑150 (MQ=60)
                                                                                                      GGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCC                                                 >  SRR19973071.3642222/1‑150 (MQ=60)
                                                                                                         ATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGATCCTAGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGAGGCTCAACGCTGGGGGCATAAGCAAGATCTGGAAACGCCAGT                                              >  SRR19973071.1761703/1‑150 (MQ=60)
                                                                                                          TTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTT                                             <  SRR19973071.718432/150‑1 (MQ=60)
                                                                                                             CGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAC                                          <  SRR19973071.380060/150‑1 (MQ=60)
                                                                                                             CGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAC                                          >  SRR19973071.1833927/1‑150 (MQ=60)
                                                                                                             CGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAC                                          <  SRR19973071.4553335/150‑1 (MQ=60)
                                                                                                                TGGGGAAAATCGCCCGTCCCCAAGAGATCGCCAAAACTATTTTGTTACTCGCCTCTGACCTCGCCAGCCCTATTACACTACAGGATATTGTGGTCGATGGCGGCTCACCGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                       >  SRR19973071.1137771/1‑150 (MQ=60)
                                                                                                                TGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                       >  SRR19973071.818648/1‑150 (MQ=60)
                                                                                                                 GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTC                                      <  SRR19973071.1751984/150‑1 (MQ=60)
                                                                                                                       AATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAA                                >  SRR19973071.3258502/1‑150 (MQ=60)
                                                                                                                        ATCGCCCGTCCACAAGAGATCGCCAACACTTGTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCTTGATCTGGAAACGCCATTTAACGCTCGACGAAC                               <  SRR19973071.4248784/150‑1 (MQ=60)
                                                                                                                        ATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAAC                               >  SRR19973071.459489/1‑150 (MQ=60)
                                                                                                                         TCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACT                              >  SRR19973071.2925644/1‑150 (MQ=60)
                                                                                                                         TCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACT                              >  SRR19973071.3967755/1‑150 (MQ=60)
                                                                                                                           GCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGA                            >  SRR19973071.2416971/1‑150 (MQ=60)
                                                                                                                           GCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGA                            <  SRR19973071.1710011/150‑1 (MQ=60)
                                                                                                                             CCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAC                          >  SRR19973071.3646966/1‑150 (MQ=60)
                                                                                                                             CCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAC                          >  SRR19973071.1248909/1‑150 (MQ=60)
                                                                                                                             CCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCCGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAC                          >  SRR19973071.3119778/1‑150 (MQ=60)
                                                                                                                                 CCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCA                      >  SRR19973071.647553/1‑150 (MQ=60)
                                                                                                                                 CCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCA                      >  SRR19973071.2835571/1‑150 (MQ=60)
                                                                                                                                     AAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAG                  >  SRR19973071.89080/1‑150 (MQ=60)
                                                                                                                                     AAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAG                  >  SRR19973071.89098/1‑150 (MQ=60)
                                                                                                                                      AGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGC                 >  SRR19973071.1082382/1‑150 (MQ=60)
                                                                                                                                        AGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA               >  SRR19973071.2511802/1‑150 (MQ=60)
                                                                                                                                         GATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAT              <  SRR19973071.421250/150‑1 (MQ=60)
                                                                                                                                          ATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATA             >  SRR19973071.2102140/1‑150 (MQ=60)
                                                                                                                                          ATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATA             >  SRR19973071.2102152/1‑150 (MQ=60)
                                                                                                                                          ATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATA             <  SRR19973071.3436962/150‑1 (MQ=60)
                                                                                                                                          ATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATA             >  SRR19973071.4106287/1‑150 (MQ=60)
                                                                                                                                               CAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA        <  SRR19973071.3430697/150‑1 (MQ=60)
                                                                                                                                               CAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA        <  SRR19973071.2350753/150‑1 (MQ=60)
                                                                                                                                               CAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA        <  SRR19973071.3535528/150‑1 (MQ=60)
                                                                                                                                                 ACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAT      >  SRR19973071.2728813/1‑150 (MQ=60)
                                                                                                                                                   ACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGG    >  SRR19973071.4193415/1‑150 (MQ=60)
                                                                                                                                                   ACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGG    >  SRR19973071.4193328/1‑150 (MQ=60)
                                                                                                                                                    CTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT   <  SRR19973071.2000657/150‑1 (MQ=60)
                                                                                                                                                    CTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT   >  SRR19973071.268969/1‑150 (MQ=60)
                                                                                                                                                    CTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT   >  SRR19973071.269837/1‑150 (MQ=60)
                                                                                                                                                     GATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  <  SRR19973071.4626671/150‑1 (MQ=60)
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GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGTG  >  CP009273/624520‑624818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑