Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 624,669 G→T T221T (ACG→ACT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273624,6690GT100.0% 691.3 / NA 213T221T (ACG→ACT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (85/128);  total (85/128)

Only 100 of 213 total aligned reads displayed.

TTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATG  >  CP009273/624522‑624815
                                                                                                                                                   |                                                                                                                                                  
  cGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                >  1:1112680/1‑150 (MQ=255)
  cGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                <  2:1250257/150‑1 (MQ=255)
  cGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                                <  2:380247/150‑1 (MQ=255)
   gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtttt                                                                                                                                               <  1:919276/150‑1 (MQ=255)
   gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtttt                                                                                                                                               <  1:883418/150‑1 (MQ=255)
   gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtttt                                                                                                                                               <  2:3136580/150‑1 (MQ=255)
   gCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtttt                                                                                                                                               <  1:2560537/150‑1 (MQ=255)
    ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                              <  2:1441526/150‑1 (MQ=255)
      tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                            <  2:413309/150‑1 (MQ=255)
      tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                            <  1:1337427/150‑1 (MQ=255)
         cTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCt                                                                                                                                         <  1:2331895/150‑1 (MQ=255)
          tCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTc                                                                                                                                        >  2:1332721/1‑150 (MQ=255)
          tCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTc                                                                                                                                        <  1:441375/150‑1 (MQ=255)
          tCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTc                                                                                                                                        <  1:321881/150‑1 (MQ=255)
           ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                       >  2:1891972/1‑150 (MQ=255)
           ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCg                                                                                                                                       >  1:2186849/1‑150 (MQ=255)
                gacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctct                                                                                                                                  <  2:164722/150‑1 (MQ=255)
                      gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTc                                                                                                                            <  1:3408434/150‑1 (MQ=255)
                         atGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgcc                                                                                                                         <  2:2027140/150‑1 (MQ=255)
                          tGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgcca                                                                                                                        <  1:3284157/150‑1 (MQ=255)
                             aaCGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                     <  1:3672088/150‑1 (MQ=255)
                                 cacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCatat                                                                                                                 <  2:2506926/150‑1 (MQ=255)
                                   cgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTa                                                                                                               >  1:2180633/1‑150 (MQ=255)
                                   cgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTa                                                                                                               <  2:1295935/150‑1 (MQ=255)
                                       gtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCt                                                                                                           <  1:524962/150‑1 (MQ=255)
                                       gtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCt                                                                                                           <  1:3296422/150‑1 (MQ=255)
                                          ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACa                                                                                                        <  2:2571233/150‑1 (MQ=255)
                                          ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACa                                                                                                        <  1:3134807/150‑1 (MQ=255)
                                              aGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                    >  1:3030770/1‑150 (MQ=255)
                                                cGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGatat                                                                                                  >  1:2090654/1‑150 (MQ=255)
                                                 gATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAtt                                                                                                 >  2:1746205/1‑150 (MQ=255)
                                                      cGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGCTATTGTGGt                                                                                            >  2:2659625/1‑150 (MQ=255)
                                                       gCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTc                                                                                           <  2:2871064/150‑1 (MQ=255)
                                                       gCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTc                                                                                           <  2:1492737/150‑1 (MQ=255)
                                                        ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCg                                                                                          <  2:427868/150‑1 (MQ=255)
                                                          gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                        >  1:2737138/1‑150 (MQ=255)
                                                          gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                        >  1:863846/1‑150 (MQ=255)
                                                          gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                        <  1:3762785/150‑1 (MQ=255)
                                                          gaagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCATATGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                        >  1:2696016/1‑150 (MQ=255)
                                                           aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATg                                                                                       >  1:2914900/1‑150 (MQ=255)
                                                           aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATg                                                                                       >  2:2311725/1‑150 (MQ=255)
                                                                cAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATggcggc                                                                                  >  1:3177915/1‑150 (MQ=255)
                                                                 aGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCt                                                                                 >  1:1784599/1‑150 (MQ=255)
                                                                 aGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCt                                                                                 >  1:651103/1‑150 (MQ=255)
                                                                  gCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                <  1:356756/150‑1 (MQ=255)
                                                                  gCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTc                                                                                <  2:2186849/150‑1 (MQ=255)
                                                                  gCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCNc                                                                                <  1:2499540/150‑1 (MQ=255)
                                                                     tATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAAc                                                                             <  1:3455809/150‑1 (MQ=255)
                                                                      aTTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                            <  2:3679005/150‑1 (MQ=255)
                                                                      aTTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                            <  2:2960905/150‑1 (MQ=255)
                                                                         cgcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTg                                                                         <  1:754577/150‑1 (MQ=255)
                                                                          gcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgg                                                                        <  2:1562197/150‑1 (MQ=255)
                                                                          gcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgg                                                                        <  2:11939/150‑1 (MQ=255)
                                                                             gCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggggg                                                                     >  1:1242558/1‑150 (MQ=255)
                                                                                 tGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGATCCTCGCCTCAGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATaa                                                                >  2:134228/1‑150 (MQ=255)
                                                                                  ggCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATaa                                                                >  2:1012482/1‑150 (MQ=255)
                                                                                   gCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCGGGGGGCATAAg                                                               >  1:3064825/1‑150 (MQ=255)
                                                                                     gAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                             >  1:1509732/1‑150 (MQ=255)
                                                                                      aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                            <  1:2831355/150‑1 (MQ=255)
                                                                                      aGCAGTTTAAACGCGGCAGTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                            <  1:2124344/150‑1 (MQ=255)
                                                                                       gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATg                                                           <  2:2732054/150‑1 (MQ=255)
                                                                                            ttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTg                                                      <  1:1170105/150‑1 (MQ=255)
                                                                                              aaaCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGa                                                    >  2:1054052/1‑150 (MQ=255)
                                                                                                 cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAATAGATCTCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAc                                                 >  1:2224476/1‑150 (MQ=255)
                                                                                                 cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAc                                                 <  2:3703887/150‑1 (MQ=255)
                                                                                                 cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAc                                                 >  2:686521/1‑150 (MQ=255)
                                                                                                     gCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCa                                             >  1:2410322/1‑150 (MQ=255)
                                                                                                       aTTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAtt                                           <  1:448725/150‑1 (MQ=255)
                                                                                                        ttCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAttt                                          >  1:1357923/1‑150 (MQ=255)
                                                                                                        ttCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAttt                                          <  2:3052117/150‑1 (MQ=255)
                                                                                                         tCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTa                                         <  1:2771750/150‑1 (MQ=255)
                                                                                                             cTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGc                                     <  2:2366959/150‑1 (MQ=255)
                                                                                                              tGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                    >  2:535206/1‑150 (MQ=255)
                                                                                                              tGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                    <  1:1891843/150‑1 (MQ=255)
                                                                                                                gggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcg                                  <  1:497249/150‑1 (MQ=255)
                                                                                                                 ggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                                 <  1:1012482/150‑1 (MQ=255)
                                                                                                                 ggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                                 <  1:1636145/150‑1 (MQ=255)
                                                                                                                     aaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGaa                             >  1:1066158/1‑150 (MQ=255)
                                                                                                                        cGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTg                          >  2:3078356/1‑150 (MQ=255)
                                                                                                                           ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGACCTGAAc                       >  1:798222/1‑150 (MQ=255)
                                                                                                                           ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                       <  2:2914900/150‑1 (MQ=255)
                                                                                                                           ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAAc                       <  2:2696016/150‑1 (MQ=255)
                                                                                                                              tCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGcc                    <  2:1142204/150‑1 (MQ=255)
                                                                                                                                cacaAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac                  >  2:345570/1‑150 (MQ=255)
                                                                                                                                   aaGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg               >  2:654173/1‑150 (MQ=255)
                                                                                                                                     gagaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg             <  2:2090654/150‑1 (MQ=255)
                                                                                                                                      agaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGa            >  1:3330687/1‑150 (MQ=255)
                                                                                                                                       gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt           <  2:3650189/150‑1 (MQ=255)
                                                                                                                                       gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt           >  2:1831296/1‑150 (MQ=255)
                                                                                                                                       gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAt           >  1:1539451/1‑150 (MQ=255)
                                                                                                                                       gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATAACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAGGATCTGGACACGCCATTAAACGCTCGACGAACAGAACGCCACCAGCGAt           >  2:3138973/1‑150 (MQ=255)
                                                                                                                                        aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATa          <  2:3712587/150‑1 (MQ=255)
                                                                                                                                        aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATa          >  2:429367/1‑150 (MQ=255)
                                                                                                                                         tCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATaa         <  2:3260824/150‑1 (MQ=255)
                                                                                                                                          cGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAac        <  2:3667859/150‑1 (MQ=255)
                                                                                                                                          cGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAac        <  2:3539265/150‑1 (MQ=255)
                                                                                                                                             cAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca     >  2:1969650/1‑150 (MQ=255)
                                                                                                                                             cAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca     >  1:1906809/1‑150 (MQ=255)
                                                                                                                                              aaCACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATGTTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACaa    >  1:1313599/1‑150 (MQ=255)
                                                                                                                                                cacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAAtg  <  1:2311725/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATG  >  CP009273/624522‑624815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624520‑624817
                                                                                                                                                      |                                                                                                                                                    
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGTCAACACT                                                                                                                                                      <  SRR19973061.1929340/150‑1 (MQ=60)
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACT                                                                                                                                                      >  SRR19973061.969204/1‑150 (MQ=60)
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACT                                                                                                                                                      <  SRR19973061.12372/150‑1 (MQ=60)
   TCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATT                                                                                                                                                   <  SRR19973061.462083/150‑1 (MQ=60)
    CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTT                                                                                                                                                  >  SRR19973061.1115527/1‑150 (MQ=60)
    CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTT                                                                                                                                                  <  SRR19973061.2318588/150‑1 (MQ=60)
     GCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTT                                                                                                                                                 <  SRR19973061.921629/150‑1 (MQ=60)
     GCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTT                                                                                                                                                 <  SRR19973061.921341/150‑1 (MQ=60)
     GCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTT                                                                                                                                                 <  SRR19973061.885707/150‑1 (MQ=60)
     GCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTT                                                                                                                                                 <  SRR19973061.2567075/150‑1 (MQ=60)
     GCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTT                                                                                                                                                 <  SRR19973061.2567087/150‑1 (MQ=60)
        TGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTT                                                                                                                                              <  SRR19973061.1340793/150‑1 (MQ=60)
         GGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTC                                                                                                                                             >  SRR19973061.3060001/1‑150 (MQ=60)
           CTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCT                                                                                                                                           <  SRR19973061.2878492/150‑1 (MQ=60)
           CTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCT                                                                                                                                           <  SRR19973061.2337813/150‑1 (MQ=60)
            TCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTC                                                                                                                                          <  SRR19973061.443982/150‑1 (MQ=60)
            TCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTC                                                                                                                                          <  SRR19973061.442574/150‑1 (MQ=60)
            TCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTC                                                                                                                                          <  SRR19973061.322720/150‑1 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                         >  SRR19973061.1324597/1‑150 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                         >  SRR19973061.1145108/1‑150 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                         >  SRR19973061.2192348/1‑150 (MQ=60)
             CCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCG                                                                                                                                         >  SRR19973061.2191502/1‑150 (MQ=60)
              CACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGC                                                                                                                                        >  SRR19973061.3548333/1‑150 (MQ=60)
               ACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCC                                                                                                                                       >  SRR19973061.500112/1‑150 (MQ=60)
                CCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCNT                                                                                                                                      <  SRR19973061.3805003/150‑1 (MQ=60)
                 CGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTC                                                                                                                                     <  SRR19973061.983461/150‑1 (MQ=60)
                  GACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCT                                                                                                                                    <  SRR19973061.2688900/150‑1 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                  <  SRR19973061.2524827/150‑1 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                  <  SRR19973061.879595/150‑1 (MQ=60)
                    CACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCAGTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGA                                                                                                                                  <  SRR19973061.1497886/150‑1 (MQ=60)
                        GATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTC                                                                                                                              <  SRR19973061.3417188/150‑1 (MQ=60)
                        GATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTC                                                                                                                              <  SRR19973061.754325/150‑1 (MQ=60)
                         ATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCG                                                                                                                             <  SRR19973061.2155693/150‑1 (MQ=60)
                         ATATGCAACGCACGCTGTGGGTGAGCGATGACGCAGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTATAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCTCCTCTGACCTCG                                                                                                                             >  SRR19973061.69230/1‑150 (MQ=60)
                           ATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCC                                                                                                                           <  SRR19973061.1567272/150‑1 (MQ=60)
                            TGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCA                                                                                                                          <  SRR19973061.3311156/150‑1 (MQ=60)
                            TGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCA                                                                                                                          <  SRR19973061.3292616/150‑1 (MQ=60)
                               AACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGACAATCGCCCGTCCCCAAGAGATCGCCACCACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCC                                                                                                                       >  SRR19973061.3226341/1‑150 (MQ=60)
                               AACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCC                                                                                                                       <  SRR19973061.2116004/150‑1 (MQ=60)
                               AACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCC                                                                                                                       <  SRR19973061.3681476/150‑1 (MQ=60)
                                     CGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTA                                                                                                                 >  SRR19973061.2186114/1‑150 (MQ=60)
                                        TGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCC                                                                                                              <  SRR19973061.1728704/150‑1 (MQ=60)
                                         GTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCT                                                                                                             <  SRR19973061.3304908/150‑1 (MQ=60)
                                         GTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCT                                                                                                             <  SRR19973061.526349/150‑1 (MQ=60)
                                            GGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACA                                                                                                          <  SRR19973061.3142880/150‑1 (MQ=60)
                                                AGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGAT                                                                                                      >  SRR19973061.3038603/1‑150 (MQ=60)
                                                  CGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAT                                                                                                    >  SRR19973061.2095907/1‑150 (MQ=60)
                                                            GAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAT                                                                                          <  SRR19973061.3772404/150‑1 (MQ=60)
                                                            GAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAT                                                                                          >  SRR19973061.866085/1‑150 (MQ=60)
                                                            GAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAT                                                                                          >  SRR19973061.2744172/1‑150 (MQ=60)
                                                            GAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCATAT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAT                                                                                          >  SRR19973061.2702943/1‑150 (MQ=60)
                                                             AAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCCTATTACCCTACAGGATATTGTGGTCGATG                                                                                         >  SRR19973061.2922382/1‑150 (MQ=60)
                                                              AGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGG                                                                                        <  SRR19973061.811130/150‑1 (MQ=60)
                                                               GAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGC                                                                                       <  SRR19973061.154083/150‑1 (MQ=60)
                                                                 ACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGG                                                                                     >  SRR19973061.1250475/1‑150 (MQ=60)
                                                                  CAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGC                                                                                    >  SRR19973061.3186065/1‑150 (MQ=60)
                                                                   AGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCT                                                                                   >  SRR19973061.1789069/1‑150 (MQ=60)
                                                                   AGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCT                                                                                   >  SRR19973061.652772/1‑150 (MQ=60)
                                                                    GCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTC                                                                                  <  SRR19973061.357719/150‑1 (MQ=60)
                                                                    GCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCNC                                                                                  <  SRR19973061.2505904/150‑1 (MQ=60)
                                                                     CGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTC                                                                                  <  SRR19973061.358121/150‑1 (MQ=60)
                                                                       TATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAAC                                                                               <  SRR19973061.3463707/150‑1 (MQ=60)
                                                                       TATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAAC                                                                               <  SRR19973061.3464674/150‑1 (MQ=60)
                                                                        ATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACG                                                                              <  SRR19973061.2735654/150‑1 (MQ=60)
                                                                           CGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTG                                                                           <  SRR19973061.756560/150‑1 (MQ=60)
                                                                              GGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGG                                                                        >  SRR19973061.3301029/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                       >  SRR19973061.1245519/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                       >  SRR19973061.1245639/1‑150 (MQ=60)
                                                                               GCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGG                                                                       <  SRR19973061.1902107/150‑1 (MQ=60)
                                                                                 TTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCA                                                                     <  SRR19973061.3570046/150‑1 (MQ=60)
                                                                                     GCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAG                                                                 >  SRR19973061.3072834/1‑150 (MQ=60)
                                                                                     GCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCGGGGGGCATAAG                                                                 >  SRR19973061.3072739/1‑150 (MQ=60)
                                                                                     GCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAG                                                                 <  SRR19973061.151405/150‑1 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                               <  SRR19973061.1343531/150‑1 (MQ=60)
                                                                                       GAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCA                                                               >  SRR19973061.1513511/1‑150 (MQ=60)
                                                                                        AGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAT                                                              <  SRR19973061.2836139/150‑1 (MQ=60)
                                                                                        AGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAT                                                              <  SRR19973061.2838605/150‑1 (MQ=60)
                                                                                        AGCAGTTTAAACGCGGCAGTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAT                                                              <  SRR19973061.2129676/150‑1 (MQ=60)
                                                                                              TTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTG                                                        <  SRR19973061.3279622/150‑1 (MQ=60)
                                                                                              TTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTG                                                        <  SRR19973061.1173067/150‑1 (MQ=60)
                                                                                                AAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGA                                                      >  SRR19973061.2961022/1‑150 (MQ=60)
                                                                                                AAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGA                                                      <  SRR19973061.3109523/150‑1 (MQ=60)
                                                                                                   CTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAATAGATCTCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAAC                                                   >  SRR19973061.2230055/1‑150 (MQ=60)
                                                                                                     CGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGC                                                 <  SRR19973061.2433607/150‑1 (MQ=60)
                                                                                                       GCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCA                                               >  SRR19973061.2416480/1‑150 (MQ=60)
                                                                                                       GCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCA                                               <  SRR19973061.5572/150‑1 (MQ=60)
                                                                                                         ATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATT                                             <  SRR19973061.449938/150‑1 (MQ=60)
                                                                                                          TTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTT                                            >  SRR19973061.1361346/1‑150 (MQ=60)
                                                                                                           TCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTA                                           <  SRR19973061.2778884/150‑1 (MQ=60)
                                                                                                            CCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAA                                          <  SRR19973061.335593/150‑1 (MQ=60)
                                                                                                             CGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAC                                         <  SRR19973061.2158882/150‑1 (MQ=60)
                                                                                                             CGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAAC                                         <  SRR19973061.2209269/150‑1 (MQ=60)
                                                                                                              GCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACG                                        >  SRR19973061.2747440/1‑150 (MQ=60)
                                                                                                                T‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                      <  SRR19973061.778413/150‑1 (MQ=60)
                                                                                                                T‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                      <  SRR19973061.2293097/150‑1 (MQ=60)
                                                                                                                T‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                      >  SRR19973061.3143060/1‑150 (MQ=60)
                                                                                                                T‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGGTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCT                                      <  SRR19973061.1896541/150‑1 (MQ=60)
                                                                                                                   GGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCG                                    <  SRR19973061.498563/150‑1 (MQ=60)
                                                                                                                    GGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGA                                   <  SRR19973061.1640243/150‑1 (MQ=60)
                                                                                                                    GGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGA                                   <  SRR19973061.1015063/150‑1 (MQ=60)
                                                                                                                        AATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAA                               >  SRR19973061.1068896/1‑150 (MQ=60)
                                                                                                                        AATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAA                               >  SRR19973061.1069407/1‑150 (MQ=60)
                                                                                                                           CGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTG                            <  SRR19973061.1820120/150‑1 (MQ=60)
                                                                                                                              CCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGACCTGAAC                         >  SRR19973061.800313/1‑150 (MQ=60)
                                                                                                                               CGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACG                        <  SRR19973061.1042819/150‑1 (MQ=60)
                                                                                                                                     CAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCA                  <  SRR19973061.3716252/150‑1 (MQ=60)
                                                                                                                                       AGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGACGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCCTTTAACGCTCGACGAACTCACCGCCACCAGC                >  SRR19973061.2415090/1‑150 (MQ=60)
                                                                                                                                         AGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGA              >  SRR19973061.3339268/1‑150 (MQ=60)
                                                                                                                                          GATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGAT             >  SRR19973061.1543285/1‑150 (MQ=60)
                                                                                                                                                CAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA       >  SRR19973061.1911586/1‑150 (MQ=60)
                                                                                                                                                CAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA       >  SRR19973061.1911531/1‑150 (MQ=60)
                                                                                                                                                 AACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATGTTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAA      >  SRR19973061.1316894/1‑150 (MQ=60)
                                                                                                                                                   CACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATG    <  SRR19973061.2317587/150‑1 (MQ=60)
                                                                                                                                                     CTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  SRR19973061.2414162/1‑150 (MQ=60)
                                                                                                                                                     CTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  SRR19973061.2414167/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
GTTTCGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCT‑GGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACAATGGT  >  CP009273/624520‑624817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑